Csa5G633130 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa5G633130
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionMatrix metalloproteinase; contains IPR021190 (Peptidase M10A), IPR024079 (Metallopeptidase, catalytic domain)
LocationChr5 : 25899476 .. 25900234 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTCAAGTCTCTCCAACTGCTCTTTCTTGTTTTCGTCTCCATTGCATCTCACGTAATCTCCAAACACAACCCAATTCTATCTCCTCAACGTCTCTATGGATGTCGTAAAGGCGACAACGTAGAAGGAATACGTAACATTAAAAAATACCTTCAACGTTATGGTTACTTATCACACAACATGAGTATTGATTCCCATATCATTGAACTCAATAGCAACAAGTTCGACGATTCCCTAGAATCTGCCATTAAATTATATCAGAAATGGACTCACCTCAACGTAAGTGGGGTAAAGCCCAACCTGACCTTCAATTTCACTAATTGATTAAAGCTATATGTTTCAGGGAGGGCTTGTAAAAGCTGGGCTTGAAGTTGCCCAAATATGGCTCGATGGGGAGGCCCGTCACTATTCACATTCTCAGAAGCTTCGGATGCCCAAAGTGCTGACATTAATATAAGCTTTCAGATAAAGGATCATGCAGATGGATTGCCTTTTGATGGACCTGGAGGAATTGTGAGTCATGCTTTTGCACCAACTGATGGGAGATTGCACTTAGATGGCGACGATAGTTGGTCGGCAGGGTTGGAAGAAAAAAAGGTTAATGTGATGAATGCGGCACTTCATGAGCTTGGACATGTTCTTGGTCTTGCCCATAGCACCCTTCCACAAGCTGTCATGTGGCCGTATATTGAATCCAATGCTTTAAAGAACTTGAATGATGATGATATTGCCGGACTTCATGCTTTGTATCCTTAA

mRNA sequence

ATGGCTTTCAAGTCTCTCCAACTGCTCTTTCTTGTTTTCGTCTCCATTGCATCTCACGTAATCTCCAAACACAACCCAATTCTATCTCCTCAACGTCTCTATGGATGTCGTAAAGGCGACAACGTAGAAGGAATACGTAACATTAAAAAATACCTTCAACGTTATGGTTACTTATCACACAACATGAGTATTGATTCCCATATCATTGAACTCAATAGCAACAAGTTCGACGATTCCCTAGAATCTGCCATTAAATTATATCAGAAATGGACTCACCTCAACCTATATGTTTCAGGGAGGGCTTGCCCGTCACTATTCACATTCTCAGAAGCTTCGGATGCCCAAAGTGCTGACATTAATATAAGCTTTCAGATAAAGGATCATGCAGATGGATTGCCTTTTGATGGACCTGGAGGAATTGTGAGTCATGCTTTTGCACCAACTGATGGGAGATTGCACTTAGATGGCGACGATAGTTGGTCGGCAGGGTTGGAAGAAAAAAAGGTTAATGTGATGAATGCGGCACTTCATGAGCTTGGACATGTTCTTGGTCTTGCCCATAGCACCCTTCCACAAGCTGTCATGTGGCCGTATATTGAATCCAATGCTTTAAAGAACTTGAATGATGATGATATTGCCGGACTTCATGCTTTGTATCCTTAA

Coding sequence (CDS)

ATGGCTTTCAAGTCTCTCCAACTGCTCTTTCTTGTTTTCGTCTCCATTGCATCTCACGTAATCTCCAAACACAACCCAATTCTATCTCCTCAACGTCTCTATGGATGTCGTAAAGGCGACAACGTAGAAGGAATACGTAACATTAAAAAATACCTTCAACGTTATGGTTACTTATCACACAACATGAGTATTGATTCCCATATCATTGAACTCAATAGCAACAAGTTCGACGATTCCCTAGAATCTGCCATTAAATTATATCAGAAATGGACTCACCTCAACCTATATGTTTCAGGGAGGGCTTGCCCGTCACTATTCACATTCTCAGAAGCTTCGGATGCCCAAAGTGCTGACATTAATATAAGCTTTCAGATAAAGGATCATGCAGATGGATTGCCTTTTGATGGACCTGGAGGAATTGTGAGTCATGCTTTTGCACCAACTGATGGGAGATTGCACTTAGATGGCGACGATAGTTGGTCGGCAGGGTTGGAAGAAAAAAAGGTTAATGTGATGAATGCGGCACTTCATGAGCTTGGACATGTTCTTGGTCTTGCCCATAGCACCCTTCCACAAGCTGTCATGTGGCCGTATATTGAATCCAATGCTTTAAAGAACTTGAATGATGATGATATTGCCGGACTTCATGCTTTGTATCCTTAA

Protein sequence

MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSHNMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP*
Homology
[Close]
BLAST of Csa5G633130 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 1.6e-24
Identity = 66/154 (42.86%), Postives = 88/154 (57.14%), Query Frame = 1 

Query: 75  KFDDSLESAI-KLYQKWTHL-NLYVSGRACPSLFTFSEASDAQSADINISFQIKDHADGL 134
           + DD+ +SAI + + KWT + N+            F E +  ++A+I I F  K+H D  
Sbjct: 159 RLDDTFKSAIARAFSKWTPVVNI-----------AFQETTSYETANIKILFASKNHGDPY 218

Query: 135 PFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNA------ALHELGHVLGLA 194
           PFDGPGGI+ HAFAPTDGR H D D+ W A  +  K  V +A      A+HE+GH+LGL 
Sbjct: 219 PFDGPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLG 278

Query: 195 HSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 220
           HS+  +A+M+P I     K NL  DDI G+  LY
Sbjct: 279 HSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]
BLAST of Csa5G633130 vs. Swiss-Prot
Match: 4MMP_ARATH (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana GN=4MMP PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-22
Identity = 53/118 (44.92%), Postives = 77/118 (65.25%), Query Frame = 1 

Query: 107 TFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEE 166
           +F E  D   ADI I F   DH DG PFDG  G+++H F+P +GRLHLD  ++W+   +E
Sbjct: 178 SFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDE 237

Query: 167 KK----VNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 220
           +K    V++ + A+HE+GHVLGL HS++  A M+P ++  + K NLN DD+ G+ +LY
Sbjct: 238 EKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

[Close]
BLAST of Csa5G633130 vs. Swiss-Prot
Match: HE_PARLI (Hatching enzyme OS=Paracentrotus lividus PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.6e-22
Identity = 58/151 (38.41%), Postives = 79/151 (52.32%), Query Frame = 1 

Query: 70  ELNSNKFDDSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADINISFQIKDHA 129
           +LN N   D +  A +++         VSG       +F E  D  S DI I F   DH 
Sbjct: 190 DLNQNDVKDEIRRAFRVWDD-------VSG------LSFREVPDTTSVDIRIKFGSYDHG 249

Query: 130 DGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGLAHST 189
           DG+ FDG GG+++HAF P +G  H D  ++W+ G      N+   A HE GH LGL HST
Sbjct: 250 DGISFDGRGGVLAHAFLPRNGDAHFDDSETWTEG-TRSGTNLFQVAAHEFGHSLGLYHST 309

Query: 190 LPQAVMWPYIESNALK-NLNDDDIAGLHALY 220
           +  A+M+PY +       L++DDIAG+ +LY
Sbjct: 310 VRSALMYPYYQGYVPNFRLDNDDIAGIRSLY 326

[Close]
BLAST of Csa5G633130 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 9.7e-22
Identity = 54/119 (45.38%), Postives = 71/119 (59.66%), Query Frame = 1 

Query: 107 TFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSA---- 166
           +F E  D  +AD+ I F   DH DGLPFDG  G ++HAFAP +GRLHLD  ++W      
Sbjct: 200 SFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDL 259

Query: 167 -GLEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 220
            G  E  V++ + A HE+GH+LGL HS+   AVM+P +     K +L  DD+AG+  LY
Sbjct: 260 KGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318


HSP 2 Score: 47.4 bits (111), Expect = 2.4e-04
Identity = 29/76 (38.16%), Postives = 41/76 (53.95%), Query Frame = 1 

Query: 32  RLYGCRKGDNVEGIRNIKKYLQRYGYLSHNMSIDSHIIELNSNKFDDSLESAIKLYQKWT 91
           RL   + G +V G+  +K+YL R+GY++          E+ S+ FD  LESAI LYQ+  
Sbjct: 57  RLVDVQIGSHVSGVSELKRYLHRFGYVNDGS-------EIFSDVFDGPLESAISLYQE-- 116

Query: 92  HLNLYVSGRACPSLFT 108
           +L L ++GR   S  T
Sbjct: 117 NLGLPITGRLDTSTVT 123

[Close]
BLAST of Csa5G633130 vs. Swiss-Prot
Match: D2MP_DROME (Matrix metalloproteinase-2 OS=Drosophila melanogaster GN=Mmp2 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.2e-21
Identity = 58/123 (47.15%), Postives = 72/123 (58.54%), Query Frame = 1 

Query: 104 SLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGR---LHLDGDDSW 163
           S  TF E    Q ADI I F  + H DG  FDGPG +++HAF P +GR    H D D++W
Sbjct: 179 SKLTFREVYSDQ-ADIQILFARRAHGDGYKFDGPGQVLAHAFYPGEGRGGDAHFDADETW 238

Query: 164 SAGLEEKK---VNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLH 220
           +   E       N +N ALHELGH LGLAHS +P AVM+P+ ++N +  NL DDD  G+ 
Sbjct: 239 NFDGESDDSHGTNFLNVALHELGHSLGLAHSAIPDAVMFPWYQNNEVAGNLPDDDRYGIQ 298

[Close]
BLAST of Csa5G633130 vs. TrEMBL
Match: A0A0A0KS21_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633130 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 1.4e-123
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 1 

Query: 1   MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH 60
           MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH 60

Query: 61  NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADIN 120
           NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADIN
Sbjct: 61  NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADIN 120

Query: 121 ISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELG 180
           ISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELG
Sbjct: 121 ISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELG 180

Query: 181 HVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 221
           HVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP
Sbjct: 181 HVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 220

[Close]
BLAST of Csa5G633130 vs. TrEMBL
Match: A0A0A0KX71_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G631560 PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 5.3e-59
Identity = 117/155 (75.48%), Postives = 127/155 (81.94%), Query Frame = 1 

Query: 67  HIIELNSNKFD-DSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADINISFQI 126
           H+  + +N F  D + S  +   +W          A  SLFTFSEASDAQSADINISFQ+
Sbjct: 151 HLTYMFTNNFPIDFVPSMTEAMARW----------AAQSLFTFSEASDAQSADINISFQV 210

Query: 127 KDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGL 186
           KDHADG  FDGPGG+V HAFAPTDGRLHLDGDD+WSAGLEEKKVNVMNAALHELGHVLGL
Sbjct: 211 KDHADGFAFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGLEEKKVNVMNAALHELGHVLGL 270

Query: 187 AHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 221
           AHSTLPQAVMWPYIESNALKNLNDDDIAG+HALYP
Sbjct: 271 AHSTLPQAVMWPYIESNALKNLNDDDIAGIHALYP 295

[Close]
BLAST of Csa5G633130 vs. TrEMBL
Match: A0A0A0KX71_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G631560 PE=3 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.0e-38
Identity = 81/95 (85.26%), Postives = 88/95 (92.63%), Query Frame = 1 

Query: 1  MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH 60
          MAFKSLQLLFL+  SIASHVISKHN ILSP+RLYGCRK DNVEGI +IKKYLQRYGYLSH
Sbjct: 1  MAFKSLQLLFLILASIASHVISKHNSILSPRRLYGCRKDDNVEGIHSIKKYLQRYGYLSH 60

Query: 61 NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNL 96
          N S DS+IIELNSNKFDDSLESAI+LYQKW+HLN+
Sbjct: 61 NTSTDSNIIELNSNKFDDSLESAIRLYQKWSHLNV 95


HSP 2 Score: 233.8 bits (595), Expect = 2.0e-58
Identity = 116/155 (74.84%), Postives = 127/155 (81.94%), Query Frame = 1 

Query: 67  HIIELNSNKFD-DSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADINISFQI 126
           H+  + +N F  D + S  +   +W          A  SLFTFSEASDAQSADINISFQI
Sbjct: 151 HLTYVFTNNFPVDFVPSVTEAMARW----------AAQSLFTFSEASDAQSADINISFQI 210

Query: 127 KDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGL 186
           KDHADGLPFDGPGG+V HAFAPTDGRLHLDGDD+WSAG+E +KVNVMN ALHELGHVLGL
Sbjct: 211 KDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGMEVQKVNVMNVALHELGHVLGL 270

Query: 187 AHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 221
           AHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 271 AHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 295

[Close]
BLAST of Csa5G633130 vs. TrEMBL
Match: A0A0A0KNY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.5e-42
Identity = 87/95 (91.58%), Postives = 91/95 (95.79%), Query Frame = 1 

Query: 1  MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH 60
          MAFKSLQLLFLV V+IASHV SKHNPILSPQRLYGCRKGDNVEGI +IKKYLQRYGYLSH
Sbjct: 1  MAFKSLQLLFLVLVTIASHVSSKHNPILSPQRLYGCRKGDNVEGIHSIKKYLQRYGYLSH 60

Query: 61 NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNL 96
          N SIDSHIIELNSNKFDDSLESAIKLYQKW+HLN+
Sbjct: 61 NTSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNV 95


HSP 2 Score: 230.7 bits (587), Expect = 1.7e-57
Identity = 115/155 (74.19%), Postives = 124/155 (80.00%), Query Frame = 1 

Query: 67  HIIELNSNKFD-DSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADINISFQI 126
           H+  + +N F  D + S  +   +W          A  SLFTFSEASDAQSADINISFQ+
Sbjct: 150 HLTYMFTNNFPVDFVPSVTEAMARW----------AAQSLFTFSEASDAQSADINISFQV 209

Query: 127 KDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGL 186
           KDHADG  FDGPGG+V HAFAPTDGRLHLDGDDSWSAGLEE K NVMN ALHELGHVLGL
Sbjct: 210 KDHADGSAFDGPGGVVGHAFAPTDGRLHLDGDDSWSAGLEENKFNVMNVALHELGHVLGL 269

Query: 187 AHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 221
           AHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 270 AHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 294

[Close]
BLAST of Csa5G633130 vs. TrEMBL
Match: A0A0A0KVD8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633150 PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.1e-35
Identity = 78/92 (84.78%), Postives = 84/92 (91.30%), Query Frame = 1 

Query: 4  KSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSHNMS 63
          KSLQLLFLV  SIASHVIS +N ILSP+RL GCRKGDNVEGI NIKKYLQRYGYLSHN S
Sbjct: 3  KSLQLLFLVLASIASHVISINNQILSPRRLNGCRKGDNVEGIHNIKKYLQRYGYLSHNTS 62

Query: 64 IDSHIIELNSNKFDDSLESAIKLYQKWTHLNL 96
           DS+IIELNSNKFDDSLESAI+LYQKW+HLN+
Sbjct: 63 TDSNIIELNSNKFDDSLESAIRLYQKWSHLNV 94


HSP 2 Score: 155.6 bits (392), Expect = 7.0e-35
Identity = 98/249 (39.36%), Postives = 132/249 (53.01%), Query Frame = 1 

Query: 8   LLFLVFVSIASHVISKHNPI-LSP----QRLYGCRKGDNVEGIRNIKKYLQRYGYLSHNM 67
           LLFL F S +  V ++ NP   SP    ++L GCRKGD V+GI  +K YL R+GYLS   
Sbjct: 14  LLFL-FPSFSHTVFARPNPENQSPFAFLKQLEGCRKGDKVKGIGELKMYLIRFGYLSSTD 73

Query: 68  SIDSHIIE-LNSNKFDDSLESAIKLYQ------------KWTHLNLYV------------ 127
                  E  N + FDD   S    +             KW      +            
Sbjct: 74  PNHGGPQENYNDDYFDDRNHSGSSSFHALPRYAFFNGRPKWPPSKTTLTYAFPPGTRPDA 133

Query: 128 --------SGRACPSLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTD 187
                   S  A  S F F    D ++AD+ ISFQ + H DG+PFDGP G++++AFAPTD
Sbjct: 134 MAPVAQAFSTWASVSKFQFRLVQDYKNADLKISFQRRIHGDGVPFDGPRGVLAYAFAPTD 193

Query: 188 GRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLND 219
           GRLH  GD++W+ G +    ++    LHE+GH+LGL HST+ QA  +P+IE   +K LND
Sbjct: 194 GRLHFAGDENWTVGAKPGAFDIGTIGLHEIGHLLGLKHSTVKQAKTFPFIEPGKIKGLND 253

[Close]
BLAST of Csa5G633130 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 108.2 bits (269), Expect = 6.5e-24
Identity = 53/118 (44.92%), Postives = 77/118 (65.25%), Query Frame = 1 

Query: 107 TFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEE 166
           +F E  D   ADI I F   DH DG PFDG  G+++H F+P +GRLHLD  ++W+   +E
Sbjct: 178 SFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDE 237

Query: 167 KK----VNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 220
           +K    V++ + A+HE+GHVLGL HS++  A M+P ++  + K NLN DD+ G+ +LY
Sbjct: 238 EKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

[Close]
BLAST of Csa5G633130 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 105.1 bits (261), Expect = 5.5e-23
Identity = 54/119 (45.38%), Postives = 71/119 (59.66%), Query Frame = 1 

Query: 107 TFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSA---- 166
           +F E  D  +AD+ I F   DH DGLPFDG  G ++HAFAP +GRLHLD  ++W      
Sbjct: 200 SFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDL 259

Query: 167 -GLEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 220
            G  E  V++ + A HE+GH+LGL HS+   AVM+P +     K +L  DD+AG+  LY
Sbjct: 260 KGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318


HSP 2 Score: 47.4 bits (111), Expect = 1.4e-05
Identity = 29/76 (38.16%), Postives = 41/76 (53.95%), Query Frame = 1 

Query: 32  RLYGCRKGDNVEGIRNIKKYLQRYGYLSHNMSIDSHIIELNSNKFDDSLESAIKLYQKWT 91
           RL   + G +V G+  +K+YL R+GY++          E+ S+ FD  LESAI LYQ+  
Sbjct: 57  RLVDVQIGSHVSGVSELKRYLHRFGYVNDGS-------EIFSDVFDGPLESAISLYQE-- 116

Query: 92  HLNLYVSGRACPSLFT 108
           +L L ++GR   S  T
Sbjct: 117 NLGLPITGRLDTSTVT 123

[Close]
BLAST of Csa5G633130 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 96.3 bits (238), Expect = 2.5e-20
Identity = 50/122 (40.98%), Postives = 75/122 (61.48%), Query Frame = 1 

Query: 106 FTFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSW--SAG 165
           FT SE+    ++DI I F   DH DG PFDG  G ++HAF+P  G+ HLD D++W  S  
Sbjct: 204 FTLSESFS--TSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGD 263

Query: 166 LEE-----KKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHA 220
           L+        V++ + A+HE+GH+LGL HS++ +++M+P I +   K +L +DD+ G+  
Sbjct: 264 LDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQY 323


HSP 2 Score: 53.5 bits (127), Expect = 1.9e-07
Identity = 28/65 (43.08%), Postives = 39/65 (60.00%), Query Frame = 1 

Query: 35  GCRKGDNVEGIRNIKKYLQRYGYLSHNMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLN 94
           GC  G NV+G+  IKKY QR+GY+    S +       ++ FDD L++A++LYQ  T+ N
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFSGNF------TDDFDDILKAAVELYQ--TNFN 105

Query: 95  LYVSG 100
           L V+G
Sbjct: 109 LNVTG 105

[Close]
BLAST of Csa5G633130 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 94.4 bits (233), Expect = 9.7e-20
Identity = 46/122 (37.70%), Postives = 71/122 (58.20%), Query Frame = 1 

Query: 107 TFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLE- 166
           TF+      ++DI+I F   +H DG PFDGP   ++HAF+P  G  HLDG+++W    E 
Sbjct: 207 TFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEG 266

Query: 167 -------EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHA 220
                   + V++ + A+HE+GH+LGL HS++  ++M+P I +   K +L  DD+ G+  
Sbjct: 267 GDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQY 326


HSP 2 Score: 42.4 bits (98), Expect = 4.4e-04
Identity = 21/71 (29.58%), Postives = 40/71 (56.34%), Query Frame = 1 

Query: 25  NPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSHNMSIDSHIIELNSNKFDDSLESAI 84
           NP  S     GC  G   +G+  +K+Y Q +GY++     ++++    ++ FDD L++A+
Sbjct: 41  NPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYIT-----ETNLSGNFTDDFDDILKNAV 100

Query: 85  KLYQKWTHLNL 96
           ++YQ+   LN+
Sbjct: 101 EMYQRNFQLNV 106

[Close]
BLAST of Csa5G633130 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 89.0 bits (219), Expect = 4.1e-18
Identity = 50/122 (40.98%), Postives = 68/122 (55.74%), Query Frame = 1 

Query: 108 FSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSW--SAGLE 167
           F+ +     ADI I F   +H DG PFDG  G ++HA +P  G LHLDGD+ W  S G  
Sbjct: 192 FTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEI 251

Query: 168 EKK-------VNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHA 220
            ++       V++ + A+HE+GH+LGL HS++  A+M+P I     K  L  DDI G+  
Sbjct: 252 SRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQH 311


HSP 2 Score: 49.3 bits (116), Expect = 3.6e-06
Identity = 25/69 (36.23%), Postives = 41/69 (59.42%), Query Frame = 1 

Query: 32  RLYGCRKGDNVEGIRNIKKYLQRYGYLSHNMSIDSHIIELNSNKFDDSLESAIKLYQKWT 91
           +L GC  G+N+ G+  +K+Y +R+GY++   +         ++ FDD L+SAI  YQK  
Sbjct: 47  KLAGCHIGENINGLSKLKQYFRRFGYITTTGNC--------TDDFDDVLQSAINTYQK-- 105

Query: 92  HLNLYVSGR 101
           + NL V+G+
Sbjct: 107 NFNLKVTGK 105

[Close]
BLAST of Csa5G633130 vs. NCBI nr
Match: gi|700197238|gb|KGN52415.1| (hypothetical protein Csa_5G633130 [Cucumis sativus])

HSP 1 Score: 450.3 bits (1157), Expect = 2.0e-123
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 1 

Query: 1   MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH 60
           MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH 60

Query: 61  NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADIN 120
           NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADIN
Sbjct: 61  NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADIN 120

Query: 121 ISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELG 180
           ISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELG
Sbjct: 121 ISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELG 180

Query: 181 HVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 221
           HVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP
Sbjct: 181 HVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 220

[Close]
BLAST of Csa5G633130 vs. NCBI nr
Match: gi|778708748|ref|XP_004142458.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 245.4 bits (625), Expect = 9.6e-62
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 1 

Query: 103 PSLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSA 162
           PSLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSA
Sbjct: 7   PSLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSA 66

Query: 163 GLEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 221
           GLEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP
Sbjct: 67  GLEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 124

[Close]
BLAST of Csa5G633130 vs. NCBI nr
Match: gi|778708729|ref|XP_004142460.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 235.7 bits (600), Expect = 7.6e-59
Identity = 117/155 (75.48%), Postives = 127/155 (81.94%), Query Frame = 1 

Query: 67  HIIELNSNKFD-DSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADINISFQI 126
           H+  + +N F  D + S  +   +W          A  SLFTFSEASDAQSADINISFQ+
Sbjct: 151 HLTYMFTNNFPIDFVPSMTEAMARW----------AAQSLFTFSEASDAQSADINISFQV 210

Query: 127 KDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGL 186
           KDHADG  FDGPGG+V HAFAPTDGRLHLDGDD+WSAGLEEKKVNVMNAALHELGHVLGL
Sbjct: 211 KDHADGFAFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGLEEKKVNVMNAALHELGHVLGL 270

Query: 187 AHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 221
           AHSTLPQAVMWPYIESNALKNLNDDDIAG+HALYP
Sbjct: 271 AHSTLPQAVMWPYIESNALKNLNDDDIAGIHALYP 295

[Close]
BLAST of Csa5G633130 vs. NCBI nr
Match: gi|778708729|ref|XP_004142460.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 168.3 bits (425), Expect = 1.5e-38
Identity = 81/95 (85.26%), Postives = 88/95 (92.63%), Query Frame = 1 

Query: 1  MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH 60
          MAFKSLQLLFL+  SIASHVISKHN ILSP+RLYGCRK DNVEGI +IKKYLQRYGYLSH
Sbjct: 1  MAFKSLQLLFLILASIASHVISKHNSILSPRRLYGCRKDDNVEGIHSIKKYLQRYGYLSH 60

Query: 61 NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNL 96
          N S DS+IIELNSNKFDDSLESAI+LYQKW+HLN+
Sbjct: 61 NTSTDSNIIELNSNKFDDSLESAIRLYQKWSHLNV 95


HSP 2 Score: 233.8 bits (595), Expect = 2.9e-58
Identity = 116/155 (74.84%), Postives = 127/155 (81.94%), Query Frame = 1 

Query: 67  HIIELNSNKFD-DSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADINISFQI 126
           H+  + +N F  D + S  +   +W          A  SLFTFSEASDAQSADINISFQI
Sbjct: 151 HLTYVFTNNFPVDFVPSVTEAMARW----------AAQSLFTFSEASDAQSADINISFQI 210

Query: 127 KDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGL 186
           KDHADGLPFDGPGG+V HAFAPTDGRLHLDGDD+WSAG+E +KVNVMN ALHELGHVLGL
Sbjct: 211 KDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGMEVQKVNVMNVALHELGHVLGL 270

Query: 187 AHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 221
           AHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 271 AHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 295

[Close]
BLAST of Csa5G633130 vs. NCBI nr
Match: gi|778708586|ref|XP_004142843.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 179.9 bits (455), Expect = 5.0e-42
Identity = 87/95 (91.58%), Postives = 91/95 (95.79%), Query Frame = 1 

Query: 1  MAFKSLQLLFLVFVSIASHVISKHNPILSPQRLYGCRKGDNVEGIRNIKKYLQRYGYLSH 60
          MAFKSLQLLFLV V+IASHV SKHNPILSPQRLYGCRKGDNVEGI +IKKYLQRYGYLSH
Sbjct: 1  MAFKSLQLLFLVLVTIASHVSSKHNPILSPQRLYGCRKGDNVEGIHSIKKYLQRYGYLSH 60

Query: 61 NMSIDSHIIELNSNKFDDSLESAIKLYQKWTHLNL 96
          N SIDSHIIELNSNKFDDSLESAIKLYQKW+HLN+
Sbjct: 61 NTSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNV 95


HSP 2 Score: 230.7 bits (587), Expect = 2.4e-57
Identity = 115/155 (74.19%), Postives = 124/155 (80.00%), Query Frame = 1 

Query: 67  HIIELNSNKFD-DSLESAIKLYQKWTHLNLYVSGRACPSLFTFSEASDAQSADINISFQI 126
           H+  + +N F  D + S  +   +W          A  SLFTFSEASDAQSADINISFQ+
Sbjct: 150 HLTYMFTNNFPVDFVPSVTEAMARW----------AAQSLFTFSEASDAQSADINISFQV 209

Query: 127 KDHADGLPFDGPGGIVSHAFAPTDGRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGL 186
           KDHADG  FDGPGG+V HAFAPTDGRLHLDGDDSWSAGLEE K NVMN ALHELGHVLGL
Sbjct: 210 KDHADGSAFDGPGGVVGHAFAPTDGRLHLDGDDSWSAGLEENKFNVMNVALHELGHVLGL 269

Query: 187 AHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 221
           AHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 270 AHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 294

The following BLAST results are available for this feature:
BLAST of Csa5G633130 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MEP1_SOYBN1.6e-2442.86Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
4MMP_ARATH1.1e-2244.92Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana GN=4MMP PE=1 SV=1[more]
HE_PARLI2.6e-2238.41Hatching enzyme OS=Paracentrotus lividus PE=1 SV=1[more]
1MMP_ARATH9.7e-2245.38Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
D2MP_DROME2.2e-2147.15Matrix metalloproteinase-2 OS=Drosophila melanogaster GN=Mmp2 PE=2 SV=1[more]
BLAST of Csa5G633130 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KS21_CUCSA1.4e-123100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633130 PE=3 SV=1[more]
A0A0A0KX71_CUCSA5.3e-5975.48Uncharacterized protein OS=Cucumis sativus GN=Csa_5G631560 PE=3 SV=1[more]
A0A0A0KX71_CUCSA1.0e-3885.26Uncharacterized protein OS=Cucumis sativus GN=Csa_5G631560 PE=3 SV=1[more]
A0A0A0KNY8_CUCSA3.5e-4291.58Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1[more]
A0A0A0KVD8_CUCSA1.1e-3584.78Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633150 PE=3 SV=1[more]
BLAST of Csa5G633130 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G45040.16.5e-2444.92 Matrixin family protein[more]
AT4G16640.15.5e-2345.38 Matrixin family protein[more]
AT1G70170.12.5e-2040.98 matrix metalloproteinase[more]
AT1G24140.19.7e-2037.70 Matrixin family protein[more]
AT1G59970.14.1e-1840.98 Matrixin family protein[more]
BLAST of Csa5G633130 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700197238|gb|KGN52415.1|2.0e-123100.00hypothetical protein Csa_5G633130 [Cucumis sativus][more]
gi|778708748|ref|XP_004142458.2|9.6e-62100.00PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|778708729|ref|XP_004142460.2|7.6e-5975.48PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|778708729|ref|XP_004142460.2|1.5e-3885.26PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|778708586|ref|XP_004142843.2|5.0e-4291.58PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001818Pept_M10_metallopeptidase
IPR002477Peptidoglycan-bd-like
IPR006026Peptidase_Metallo
IPR021190Pept_M10A
IPR024079MetalloPept_cat_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa5G633130.1Csa5G633130.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 63..219
score: 4.7
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 45..82
score: 3.
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 43..94
score: 7.
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 51..220
score: 2.8
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 174..199
score: 1.2E-11coord: 207..220
score: 1.2E-11coord: 80..95
score: 1.2
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 83..219
score: 3.0
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 1..219
score: 2.1
NoneNo IPR availablePANTHERPTHR10201:SF152SUBFAMILY NOT NAMEDcoord: 1..219
score: 2.1
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 64..220
score: 2.53