Csa5G632070 (gene) Cucumber (Chinese Long) v2

NameCsa5G632070
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionMatrix metalloproteinase; contains IPR021190 (Peptidase M10A), IPR024079 (Metallopeptidase, catalytic domain)
LocationChr5 : 25855284 .. 25856204 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTAAGTTATGTCTCCAACTCTTCCTACTTTTGGCCTTCGTTACACCTCATTTGACATCGGCTCGTCCTCTTCACAACCATAAATCATCTCGACTTCTATTTCCCGAGCATCTCATAGGAAGTAGTAAAAACGATAGCATCGTGGAAGGAATTCATAAAGTCAAGGCTTACCTTCAACGTTACGGTTATTTCAGCAATGAAAATGAAAACAATTTGAGCACAGATGCATTCGACGACGACTTAGAATCCGCCATTAAATCATACCAAAAATTCTCCAACCTTAAGGTAAGTGGAGTCTTAGACAGGGAGACACTACAACAAATGTCGAGGCCTCGATGTGGGGTGGCAGACAATTTCCAATCAGTAGCACAACAAGATGGTGAGAATAATAATACTACTGTTCAAATTGGTGGTTCACATTTCATGTTCTTCCCCGGCAAGGGAAAATGGCCATATAGAAAGTGGCACTTGACCTATGGATTTGTCCATAATTACCCAATGAAGCACGCCGCTGCAGTGGTTCGAGCTTTCGATAAATGGGCAGCTAATTCGAAATTTACATTCTCCCTTGCCTGGAGAATACAAACAGCAGACATTTTATTAAGTTTTGAGAGAGGGGATCATGGAGATGGGAAACCGTTTGATGGAGAAGGGGGAATATTGGGACACGCGTTTGGGCCGATCGATGGGAGAGTGCATTTTGACGCAGATGAGCAGTGGGCCGAAATAGGTTCGCTTACGAACGAAAACTTTGATTTCGAGTCTGTGGCATTGCATGAGATAGGACATGCACTTGGTCTCGGCCATAGCATTTTTCCATCTGCCGTCATGTGGGCTAATATGGAGACTGGTGTCAACAAGACTGAATTGACTATTGATGATATTGAAGGCGTTCATGCTTTAAATGACCCTTAA

mRNA sequence

ATGGCTGCTAAGTTATGTCTCCAACTCTTCCTACTTTTGGCCTTCGTTACACCTCATTTGACATCGGCTCGTCCTCTTCACAACCATAAATCATCTCGACTTCTATTTCCCGAGCATCTCATAGGAAGTAGTAAAAACGATAGCATCGTGGAAGGAATTCATAAAGTCAAGGCTTACCTTCAACGTTACGGTTATTTCAGCAATGAAAATGAAAACAATTTGAGCACAGATGCATTCGACGACGACTTAGAATCCGCCATTAAATCATACCAAAAATTCTCCAACCTTAAGGTAAGTGGAGTCTTAGACAGGGAGACACTACAACAAATGTCGAGGCCTCGATGTGGGGTGGCAGACAATTTCCAATCAGTAGCACAACAAGATGGTGAGAATAATAATACTACTGTTCAAATTGGTGGTTCACATTTCATGTTCTTCCCCGGCAAGGGAAAATGGCCATATAGAAAGTGGCACTTGACCTATGGATTTGTCCATAATTACCCAATGAAGCACGCCGCTGCAGTGGTTCGAGCTTTCGATAAATGGGCAGCTAATTCGAAATTTACATTCTCCCTTGCCTGGAGAATACAAACAGCAGACATTTTATTAAGTTTTGAGAGAGGGGATCATGGAGATGGGAAACCGTTTGATGGAGAAGGGGGAATATTGGGACACGCGTTTGGGCCGATCGATGGGAGAGTGCATTTTGACGCAGATGAGCAGTGGGCCGAAATAGGTTCGCTTACGAACGAAAACTTTGATTTCGAGTCTGTGGCATTGCATGAGATAGGACATGCACTTGGTCTCGGCCATAGCATTTTTCCATCTGCCGTCATGTGGGCTAATATGGAGACTGGTGTCAACAAGACTGAATTGACTATTGATGATATTGAAGGCGTTCATGCTTTAAATGACCCTTAA

Coding sequence (CDS)

ATGGCTGCTAAGTTATGTCTCCAACTCTTCCTACTTTTGGCCTTCGTTACACCTCATTTGACATCGGCTCGTCCTCTTCACAACCATAAATCATCTCGACTTCTATTTCCCGAGCATCTCATAGGAAGTAGTAAAAACGATAGCATCGTGGAAGGAATTCATAAAGTCAAGGCTTACCTTCAACGTTACGGTTATTTCAGCAATGAAAATGAAAACAATTTGAGCACAGATGCATTCGACGACGACTTAGAATCCGCCATTAAATCATACCAAAAATTCTCCAACCTTAAGGTAAGTGGAGTCTTAGACAGGGAGACACTACAACAAATGTCGAGGCCTCGATGTGGGGTGGCAGACAATTTCCAATCAGTAGCACAACAAGATGGTGAGAATAATAATACTACTGTTCAAATTGGTGGTTCACATTTCATGTTCTTCCCCGGCAAGGGAAAATGGCCATATAGAAAGTGGCACTTGACCTATGGATTTGTCCATAATTACCCAATGAAGCACGCCGCTGCAGTGGTTCGAGCTTTCGATAAATGGGCAGCTAATTCGAAATTTACATTCTCCCTTGCCTGGAGAATACAAACAGCAGACATTTTATTAAGTTTTGAGAGAGGGGATCATGGAGATGGGAAACCGTTTGATGGAGAAGGGGGAATATTGGGACACGCGTTTGGGCCGATCGATGGGAGAGTGCATTTTGACGCAGATGAGCAGTGGGCCGAAATAGGTTCGCTTACGAACGAAAACTTTGATTTCGAGTCTGTGGCATTGCATGAGATAGGACATGCACTTGGTCTCGGCCATAGCATTTTTCCATCTGCCGTCATGTGGGCTAATATGGAGACTGGTGTCAACAAGACTGAATTGACTATTGATGATATTGAAGGCGTTCATGCTTTAAATGACCCTTAA

Protein sequence

MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADNFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADEQWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGVHALNDP*
BLAST of Csa5G632070 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.4e-49
Identity = 108/268 (40.30%), Postives = 143/268 (53.36%), Query Frame = 1

Query: 50  VEGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQ 109
           V+G++++K Y QR+GY       N  TD FDD L++A++ YQ   NL V+G LD  T+Q 
Sbjct: 56  VDGLYRIKKYFQRFGYIPETFSGNF-TDDFDDILKAAVELYQTNFNLNVTGELDALTIQH 115

Query: 110 MSRPRCGVADNFQSVAQQDGENNNT-TVQIGGSH------FMFFPGKGKWPYRKWHLTYG 169
           +  PRCG  D     +   G    T  V    +H      +  FPG+ +WP  +  LTY 
Sbjct: 116 IVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYA 175

Query: 170 FVHNYPMKHAAAVV--RAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEG 229
           F    P+      V  RAF +W+  +   F+L+    T+DI + F  GDHGDG+PFDG  
Sbjct: 176 FDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVL 235

Query: 230 GILGHAFGPIDGRVHFDADEQWAEIGSL-----TNENFDFESVALHEIGHALGLGHSIFP 289
           G L HAF P  G+ H DADE W   G L          D ESVA+HEIGH LGLGHS   
Sbjct: 236 GTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVE 295

Query: 290 SAVMWANMETGVNKTELTIDDIEGVHAL 304
            ++M+  + TG  K +LT DD+EG+  L
Sbjct: 296 ESIMYPTITTGKRKVDLTNDDVEGIQYL 322

BLAST of Csa5G632070 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 5.8e-49
Identity = 111/263 (42.21%), Postives = 145/263 (55.13%), Query Frame = 1

Query: 50  VEGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQ 109
           + G+ K+K Y +R+GY +        TD FDD L+SAI +YQK  NLKV+G LD  TL+Q
Sbjct: 57  INGLSKLKQYFRRFGYITTTGN---CTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQ 116

Query: 110 MSRPRCGVADNFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFV--HNY 169
           + +PRCG  D    V++ +G     T +     + FFPGK +WP RK  LTY F   +N 
Sbjct: 117 IVKPRCGNPDLIDGVSEMNGGKILRTTE----KYSFFPGKPRWPKRKRDLTYAFAPQNNL 176

Query: 170 PMKHAAAVVRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAF 229
             +      RAF +WA  +   F+ +  I  ADI++ F  G+HGDG+PFDG  G L HA 
Sbjct: 177 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 236

Query: 230 GPIDGRVHFDADEQW-AEIGSLTNENF------DFESVALHEIGHALGLGHSIFPSAVMW 289
            P  G +H D DE W    G ++          D ESVA+HEIGH LGLGHS    A+M+
Sbjct: 237 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 296

Query: 290 ANMETGVNKTELTIDDIEGVHAL 304
             +  G  K EL  DDIEG+  L
Sbjct: 297 PAISGGDRKVELAKDDIEGIQHL 312

BLAST of Csa5G632070 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.9e-47
Identity = 103/269 (38.29%), Postives = 138/269 (51.30%), Query Frame = 1

Query: 51  EGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQM 110
           +G++ +K Y Q +GY +  N +   TD FDD L++A++ YQ+   L V+GVLD  TL+ +
Sbjct: 59  DGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHV 118

Query: 111 SRPRCGVADNFQSVAQQDGENNNTTVQIGG--------SHFMFFPGKGKWPYRKWHLTYG 170
             PRCG  D     +          V   G         H+ FFPG+ +WP  +  LTY 
Sbjct: 119 VIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYA 178

Query: 171 FVHNYPMKHAAAVV--RAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEG 230
           F     +      V  RAF +W   +  TF+   R  T+DI + F  G+HGDG+PFDG  
Sbjct: 179 FDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPM 238

Query: 231 GILGHAFGPIDGRVHFDADEQWAEIGS------LTNENFDFESVALHEIGHALGLGHSIF 290
             L HAF P  G  H D +E W   G         +E  D ESVA+HEIGH LGLGHS  
Sbjct: 239 RTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSV 298

Query: 291 PSAVMWANMETGVNKTELTIDDIEGVHAL 304
             ++M+  + TG  K +LT DD+EGV  L
Sbjct: 299 EGSIMYPTIRTGRRKVDLTTDDVEGVQYL 327

BLAST of Csa5G632070 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 2.3e-45
Identity = 110/260 (42.31%), Postives = 132/260 (50.77%), Query Frame = 1

Query: 51  EGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQM 110
           +G+  VK Y    GY  N    +   D FDD L SAIK+YQK  NL V+G  D  TL+Q+
Sbjct: 52  KGLSNVKNYFHHLGYIPNAPHFD---DNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQI 111

Query: 111 SRPRCGVADNFQSVAQQDGENNNTTVQIGG-SHFMFFPGKGKWPYRKWHLTYGFVHNYPM 170
             PRCGV D           N N T   G  S + FF    +W      LTY F     +
Sbjct: 112 MTPRCGVPDIII--------NTNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRL 171

Query: 171 KHA--AAVVRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAF 230
                +A+ RAF KW       F      +TA+I + F   +HGD  PFDG GGILGHAF
Sbjct: 172 DDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAF 231

Query: 231 GPIDGRVHFDADEQWAEIGSLT----NENFDFESVALHEIGHALGLGHSIFPSAVMWANM 290
            P DGR HFDADE W   G +T       FD ESVA+HEIGH LGLGHS    A+M+ ++
Sbjct: 232 APTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSI 291

Query: 291 ETGVNKTELTIDDIEGVHAL 304
                K  L  DDI+G+  L
Sbjct: 292 PPRTRKVNLAQDDIDGIRKL 300

BLAST of Csa5G632070 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.5e-44
Identity = 116/318 (36.48%), Postives = 165/318 (51.89%), Query Frame = 1

Query: 10  FLLLAFVTPHLTSAR--PLHNHKSSRLLFPEHLIGSSKND----------SIVEGIHKVK 69
           F+L+ F  P+   AR  P     +++     H+  S+ +D          S V G+ ++K
Sbjct: 15  FVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSELK 74

Query: 70  AYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGV 129
            YL R+GY ++ +E  + +D FD  LESAI  YQ+   L ++G LD  T+  MS PRCGV
Sbjct: 75  RYLHRFGYVNDGSE--IFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGV 134

Query: 130 ADNFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAA--- 189
           +D   ++   + +  +TT     +H+ +F GK KW   +  LTY     + + +  +   
Sbjct: 135 SDTHMTI---NNDFLHTT-----AHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDV 194

Query: 190 ---VVRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPID 249
                RAF +W++    +F       TAD+ + F  GDHGDG PFDG  G L HAF P +
Sbjct: 195 KTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPEN 254

Query: 250 GRVHFDADEQWAEIGSLTNEN---FDFESVALHEIGHALGLGHSIFPSAVMWANMETGVN 307
           GR+H DA E W     L   +    D ESVA HEIGH LGLGHS   SAVM+ ++     
Sbjct: 255 GRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTK 314

BLAST of Csa5G632070 vs. TrEMBL
Match: A0A0A0KS35_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 3.2e-179
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 1

Query: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60
           MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL
Sbjct: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120
           QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN
Sbjct: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120

Query: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180
           FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD
Sbjct: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180

Query: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240
           KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE
Sbjct: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240

Query: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300
           QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV
Sbjct: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300

Query: 301 HALNDP 307
           HALNDP
Sbjct: 301 HALNDP 306

BLAST of Csa5G632070 vs. TrEMBL
Match: A0A0A0KVC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632060 PE=3 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.1e-118
Identity = 211/303 (69.64%), Postives = 242/303 (79.87%), Query Frame = 1

Query: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60
           MA+KLCLQL LLLAF+TPHLTSAR LHN K S  LFP+HLIGSSKNDSIVEGIHKVKAYL
Sbjct: 1   MASKLCLQLCLLLAFITPHLTSARHLHNRKPSPFLFPKHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120
           QRYGY SNENE  LSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGV D 
Sbjct: 61  QRYGYLSNENEKKLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVRDT 120

Query: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180
           + SV QQ+ EN NT ++IGGSH+ F+     WP  + HL+YGF+H++P +H   V RAF 
Sbjct: 121 YVSVGQQEHENKNTNIEIGGSHYTFYYNHVTWPAERRHLSYGFIHDFPPQHVDTVRRAFQ 180

Query: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240
            W  N+KF+F L+ R+QTADIL+SFERG+HGD +PFDGEGGIL H+ G +DGRVHFDAD+
Sbjct: 181 TWEDNTKFSFFLSPRVQTADILVSFERGEHGDHEPFDGEGGILAHSLGAVDGRVHFDADD 240

Query: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300
           +W        E +D E++ALHEIGHALGLGHS    A+MWA M  G  KT LTIDDIEG+
Sbjct: 241 RWE---GDPMERYDLETLALHEIGHALGLGHSSSSLAIMWAYMYKGFPKTRLTIDDIEGI 300

Query: 301 HAL 304
            AL
Sbjct: 301 RAL 300

BLAST of Csa5G632070 vs. TrEMBL
Match: A0A0A0KU02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.9e-70
Identity = 151/310 (48.71%), Postives = 188/310 (60.65%), Query Frame = 1

Query: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60
           MA +  LQLFLL+A + PH+ +AR  H H SS      HL GS   ++I EGIH VK YL
Sbjct: 1   MALQFSLQLFLLIASIAPHVITARVRHTHISS------HLQGSRIGNNI-EGIHNVKLYL 60

Query: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120
           +RYGY +N    N S + FD  LESAIK++QK+ +L VSGV+D ETL  MS PRCG+ D 
Sbjct: 61  KRYGYLTNVESTN-SNNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDI 120

Query: 121 FQSVAQQDGENNNTTVQIGG--SHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRA 180
             ++   D   NN  +      SHF FFP   KWP  K++L Y F+ ++P      V+ A
Sbjct: 121 MHNINVMD---NNVQMNSSSFHSHFTFFPDNLKWPVSKYNLRYTFLDDFPNDFKEPVMNA 180

Query: 181 FDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDA 240
            ++WA  S F FS A   Q ADI  +F RG+HGDG PFDG+GG+L HAFGP+DGRVHFD 
Sbjct: 181 MEQWALFSLFRFSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDW 240

Query: 241 DEQWAE--IGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDD 300
           DE WA+  +G   N       VALHE+GH LGL HS    A+MW  ME G     L  DD
Sbjct: 241 DEDWADGSVGGFINVGM----VALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDD 295

Query: 301 IEGVHALNDP 307
           IEG+  L  P
Sbjct: 301 IEGIQTLYAP 295

BLAST of Csa5G632070 vs. TrEMBL
Match: A0A0A0KS39_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-68
Identity = 149/308 (48.38%), Postives = 188/308 (61.04%), Query Frame = 1

Query: 11  LLLAFVTPHLTSARPLHNHK----SSRLLFPEHLIGSSKNDSIVEGIHKVKAYLQRYGYF 70
           LLL    P ++S++  H+H     SS+ +F   L GS K D  VEGIH++K YL ++GY 
Sbjct: 13  LLLLTFFPLISSSQSNHSHSHGKASSQFVFLNQLYGSQKGDK-VEGIHQLKKYLHQFGYL 72

Query: 71  SNE----NENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADNFQ 130
           + +    +ENN   D FD+ LE+AIK+YQK  NLKV+G LD  T+ QMS+PRCGVAD   
Sbjct: 73  NEDAQIHSENN--DDEFDELLETAIKTYQKNYNLKVTGTLDAMTIAQMSKPRCGVADIIH 132

Query: 131 SVAQQDGENNNTTVQIG-------GSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAV 190
                        ++ G        SHF FF G  KWP  K HLTYGF+   P +  + V
Sbjct: 133 GNTWMRSSKKRKQLEHGIVGHFHSVSHFAFFDGNPKWPATKSHLTYGFLPGTPSETVSPV 192

Query: 191 VRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVH 250
            RAF  WAANS FTFS A   QT+DI + FE GDHGDG PFDG GG++ HAF P DGR H
Sbjct: 193 ARAFATWAANSHFTFSQALDNQTSDITIGFETGDHGDGYPFDGVGGVVAHAFSPSDGRFH 252

Query: 251 FDADEQWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTID 304
            DADE WA    + +++FD E+VALHEIGH LGL HS    A+MW  +  G +K  L +D
Sbjct: 253 LDADESWA--NGVVSDSFDLETVALHEIGHLLGLQHSSTEGAIMWPQVPVGASK-GLHVD 312

BLAST of Csa5G632070 vs. TrEMBL
Match: A0A0A0KNY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.5e-67
Identity = 144/308 (46.75%), Postives = 192/308 (62.34%), Query Frame = 1

Query: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60
           MA K    LFL+L  +  H++S       K + +L P+ L G  K D+ VEGIH +K YL
Sbjct: 1   MAFKSLQLLFLVLVTIASHVSS-------KHNPILSPQRLYGCRKGDN-VEGIHSIKKYL 60

Query: 61  QRYGYFSNENENN-----LSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRC 120
           QRYGY S+    +     L+++ FDD LESAIK YQK+S+L VSG+LD+ETL QM +PRC
Sbjct: 61  QRYGYLSHNTSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFKPRC 120

Query: 121 GVADNFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAV 180
           GV D F+  + ++ E++        SH+  FPG  KWP  K HLTY F +N+P+    +V
Sbjct: 121 GVRDVFKFNSNKNLEDDLEM----SSHYTLFPGNLKWPDYKRHLTYVFTNNFPVDFVPSV 180

Query: 181 VRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVH 240
             A  +WAA S FTFS A   Q+ADI +SF+  DH DG PFDG GG++GHAF P DGR+H
Sbjct: 181 TEAMARWAAQSLFTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTDGRLH 240

Query: 241 FDADEQWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTID 300
            D D+ W+    +  +  +  +VALHE+GH LGL HS  P AVMW  +++   K  L  D
Sbjct: 241 LDGDDTWS--AGMEVQKVNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALK-NLNDD 293

Query: 301 DIEGVHAL 304
           DI G+HAL
Sbjct: 301 DIAGLHAL 293

BLAST of Csa5G632070 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 196.8 bits (499), Expect = 1.9e-50
Identity = 108/268 (40.30%), Postives = 143/268 (53.36%), Query Frame = 1

Query: 50  VEGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQ 109
           V+G++++K Y QR+GY       N  TD FDD L++A++ YQ   NL V+G LD  T+Q 
Sbjct: 56  VDGLYRIKKYFQRFGYIPETFSGNF-TDDFDDILKAAVELYQTNFNLNVTGELDALTIQH 115

Query: 110 MSRPRCGVADNFQSVAQQDGENNNT-TVQIGGSH------FMFFPGKGKWPYRKWHLTYG 169
           +  PRCG  D     +   G    T  V    +H      +  FPG+ +WP  +  LTY 
Sbjct: 116 IVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYA 175

Query: 170 FVHNYPMKHAAAVV--RAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEG 229
           F    P+      V  RAF +W+  +   F+L+    T+DI + F  GDHGDG+PFDG  
Sbjct: 176 FDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVL 235

Query: 230 GILGHAFGPIDGRVHFDADEQWAEIGSL-----TNENFDFESVALHEIGHALGLGHSIFP 289
           G L HAF P  G+ H DADE W   G L          D ESVA+HEIGH LGLGHS   
Sbjct: 236 GTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVE 295

Query: 290 SAVMWANMETGVNKTELTIDDIEGVHAL 304
            ++M+  + TG  K +LT DD+EG+  L
Sbjct: 296 ESIMYPTITTGKRKVDLTNDDVEGIQYL 322

BLAST of Csa5G632070 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 196.1 bits (497), Expect = 3.3e-50
Identity = 111/263 (42.21%), Postives = 145/263 (55.13%), Query Frame = 1

Query: 50  VEGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQ 109
           + G+ K+K Y +R+GY +        TD FDD L+SAI +YQK  NLKV+G LD  TL+Q
Sbjct: 57  INGLSKLKQYFRRFGYITTTGN---CTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQ 116

Query: 110 MSRPRCGVADNFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFV--HNY 169
           + +PRCG  D    V++ +G     T +     + FFPGK +WP RK  LTY F   +N 
Sbjct: 117 IVKPRCGNPDLIDGVSEMNGGKILRTTE----KYSFFPGKPRWPKRKRDLTYAFAPQNNL 176

Query: 170 PMKHAAAVVRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAF 229
             +      RAF +WA  +   F+ +  I  ADI++ F  G+HGDG+PFDG  G L HA 
Sbjct: 177 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 236

Query: 230 GPIDGRVHFDADEQW-AEIGSLTNENF------DFESVALHEIGHALGLGHSIFPSAVMW 289
            P  G +H D DE W    G ++          D ESVA+HEIGH LGLGHS    A+M+
Sbjct: 237 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 296

Query: 290 ANMETGVNKTELTIDDIEGVHAL 304
             +  G  K EL  DDIEG+  L
Sbjct: 297 PAISGGDRKVELAKDDIEGIQHL 312

BLAST of Csa5G632070 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 191.0 bits (484), Expect = 1.1e-48
Identity = 103/269 (38.29%), Postives = 138/269 (51.30%), Query Frame = 1

Query: 51  EGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQM 110
           +G++ +K Y Q +GY +  N +   TD FDD L++A++ YQ+   L V+GVLD  TL+ +
Sbjct: 59  DGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHV 118

Query: 111 SRPRCGVADNFQSVAQQDGENNNTTVQIGG--------SHFMFFPGKGKWPYRKWHLTYG 170
             PRCG  D     +          V   G         H+ FFPG+ +WP  +  LTY 
Sbjct: 119 VIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYA 178

Query: 171 FVHNYPMKHAAAVV--RAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEG 230
           F     +      V  RAF +W   +  TF+   R  T+DI + F  G+HGDG+PFDG  
Sbjct: 179 FDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPM 238

Query: 231 GILGHAFGPIDGRVHFDADEQWAEIGS------LTNENFDFESVALHEIGHALGLGHSIF 290
             L HAF P  G  H D +E W   G         +E  D ESVA+HEIGH LGLGHS  
Sbjct: 239 RTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSV 298

Query: 291 PSAVMWANMETGVNKTELTIDDIEGVHAL 304
             ++M+  + TG  K +LT DD+EGV  L
Sbjct: 299 EGSIMYPTIRTGRRKVDLTTDDVEGVQYL 327

BLAST of Csa5G632070 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 180.6 bits (457), Expect = 1.4e-45
Identity = 116/318 (36.48%), Postives = 165/318 (51.89%), Query Frame = 1

Query: 10  FLLLAFVTPHLTSAR--PLHNHKSSRLLFPEHLIGSSKND----------SIVEGIHKVK 69
           F+L+ F  P+   AR  P     +++     H+  S+ +D          S V G+ ++K
Sbjct: 15  FVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSELK 74

Query: 70  AYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGV 129
            YL R+GY ++ +E  + +D FD  LESAI  YQ+   L ++G LD  T+  MS PRCGV
Sbjct: 75  RYLHRFGYVNDGSE--IFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGV 134

Query: 130 ADNFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAA--- 189
           +D   ++   + +  +TT     +H+ +F GK KW   +  LTY     + + +  +   
Sbjct: 135 SDTHMTI---NNDFLHTT-----AHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDV 194

Query: 190 ---VVRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPID 249
                RAF +W++    +F       TAD+ + F  GDHGDG PFDG  G L HAF P +
Sbjct: 195 KTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPEN 254

Query: 250 GRVHFDADEQWAEIGSLTNEN---FDFESVALHEIGHALGLGHSIFPSAVMWANMETGVN 307
           GR+H DA E W     L   +    D ESVA HEIGH LGLGHS   SAVM+ ++     
Sbjct: 255 GRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTK 314

BLAST of Csa5G632070 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 152.9 bits (385), Expect = 3.2e-37
Identity = 102/290 (35.17%), Postives = 136/290 (46.90%), Query Frame = 1

Query: 26  LHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLES 85
           LHN +      P      + N S    I ++K +LQ+YGY     E++      D   E 
Sbjct: 26  LHNQQIIEARNPSQF---TTNPSPDVSIPEIKRHLQQYGYLPQNKESD------DVSFEQ 85

Query: 86  AIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADNFQSVAQQDGENNNTTVQIGGSHFMF 145
           A+  YQK   L ++G  D +TL Q+  PRCG  D+ +           T     G  +++
Sbjct: 86  ALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPDDVEP---------KTAPFHTGKKYVY 145

Query: 146 FPGKGKWPYR-KWHLTYGFVHNYPMKHAAAVV------RAFDKWAANSKFTFSLAWRIQT 205
           FPG+ +W       LTY F       + A         RAF KWA+    +F        
Sbjct: 146 FPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVI 205

Query: 206 ADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADEQWA-----EIGSLTNENF 265
           ADI + F  GDHGDG+PFDG  G+L H F P +GR+H D  E WA     E  S+     
Sbjct: 206 ADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVA---V 265

Query: 266 DFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGVHAL 304
           D ESVA+HEIGH LGLGHS    A M+  ++    K  L +DD+ GV +L
Sbjct: 266 DLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSL 294

BLAST of Csa5G632070 vs. NCBI nr
Match: gi|778708735|ref|XP_004142466.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 635.6 bits (1638), Expect = 4.6e-179
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 1

Query: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60
           MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL
Sbjct: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120
           QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN
Sbjct: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120

Query: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180
           FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD
Sbjct: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180

Query: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240
           KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE
Sbjct: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240

Query: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300
           QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV
Sbjct: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300

Query: 301 HALNDP 307
           HALNDP
Sbjct: 301 HALNDP 306

BLAST of Csa5G632070 vs. NCBI nr
Match: gi|659118025|ref|XP_008458911.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 491.9 bits (1265), Expect = 8.2e-136
Identity = 242/306 (79.08%), Postives = 259/306 (84.64%), Query Frame = 1

Query: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60
           M +KLCLQLFLL AF+TPHLTSAR L NHK SRLLFPEHLIGS KND+  EGIHKVKAYL
Sbjct: 1   MGSKLCLQLFLLFAFITPHLTSARHLRNHKPSRLLFPEHLIGSRKNDNF-EGIHKVKAYL 60

Query: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120
           QRYGY S+E ENNLS DAFDDDLESAIKSYQK   L VSGVLDRETLQQM+RPRCGV D+
Sbjct: 61  QRYGYLSDEKENNLSIDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDD 120

Query: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180
           F  VAQQD ENNNT +QIGGSH+MF+P K KWP  K HLTYGF+HNYP +HAAAV+RAFD
Sbjct: 121 FILVAQQDHENNNTNIQIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAVIRAFD 180

Query: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240
            W AN+KFTFS A R+QTADILLSFERGDHGDGKPFDG GG LGHAFG IDGRVHFD DE
Sbjct: 181 TWEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVHFDGDE 240

Query: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300
           QW E GS   E FD ESVALHEIGHALGLGHS  PSAVMWANM++GV KT LTIDDIEG+
Sbjct: 241 QW-ETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANMDSGVTKTHLTIDDIEGI 300

Query: 301 HALNDP 307
           HAL  P
Sbjct: 301 HALYGP 304

BLAST of Csa5G632070 vs. NCBI nr
Match: gi|659118027|ref|XP_008458912.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 452.6 bits (1163), Expect = 5.5e-124
Identity = 219/306 (71.57%), Postives = 248/306 (81.05%), Query Frame = 1

Query: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60
           MA+KLCLQLFLLLAF+TPHLTSAR LHNHK S  LFP+HLIGSSKNDSIVEGIHKVKAYL
Sbjct: 1   MASKLCLQLFLLLAFITPHLTSARHLHNHKPSPFLFPKHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120
           QRYGY SNENENNLSTDAFDDDLESAIKSYQKFS +KVSGVLDRETLQQMS PRCGV D 
Sbjct: 61  QRYGYLSNENENNLSTDAFDDDLESAIKSYQKFSKIKVSGVLDRETLQQMSTPRCGVRDT 120

Query: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180
           F SVAQQ+ EN +T ++IGGSH+ F+    KWP  K HL+YGF+HN+P +H   V+RAF 
Sbjct: 121 FVSVAQQEHENKSTNIEIGGSHYTFYYNNVKWPAEKKHLSYGFIHNFPPQHVPTVLRAFQ 180

Query: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240
            W  N+KF+F LA R+QTADIL+SFERG+HGD +PFDG GGIL H+ G +DGRVHFDADE
Sbjct: 181 TWEDNTKFSFFLAPRVQTADILVSFERGEHGDDEPFDGPGGILAHSLGAVDGRVHFDADE 240

Query: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300
           QW   G    + +D E++ALHE+GHALGLGHS    AVMWA M  G  KT LTIDDIEG+
Sbjct: 241 QWE--GGPVEKRYDLETLALHELGHALGLGHSSSSLAVMWAYMYAGFPKTRLTIDDIEGI 300

Query: 301 HALNDP 307
            AL  P
Sbjct: 301 RALYGP 304

BLAST of Csa5G632070 vs. NCBI nr
Match: gi|659118029|ref|XP_008458913.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 448.4 bits (1152), Expect = 1.0e-122
Identity = 223/303 (73.60%), Postives = 250/303 (82.51%), Query Frame = 1

Query: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60
           MA+KLCLQLFLLLAFVTPHLTSAR LHNHK SRLLFPEHLIGSSKND+IVEGIHKVKAYL
Sbjct: 1   MASKLCLQLFLLLAFVTPHLTSARHLHNHKPSRLLFPEHLIGSSKNDNIVEGIHKVKAYL 60

Query: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120
           QRYGYFSNEN N LSTD FD+DLESAIKSYQKFS LKVSGVLDRETLQQMS+PRCGV D 
Sbjct: 61  QRYGYFSNENANTLSTDVFDNDLESAIKSYQKFSKLKVSGVLDRETLQQMSKPRCGVRDT 120

Query: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180
           F SVAQQ+ EN NT ++IGGSH+ F+     WP  K HL+YGF+HNYP +    +VRAF+
Sbjct: 121 FVSVAQQEHENKNTNIEIGGSHYTFYFNNVTWPAEKKHLSYGFIHNYPPQFVDPLVRAFE 180

Query: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240
            W  N+KF+F LA R+QTADIL+SFERGDHGD + FDGEGGIL H+ G +DGRVHFDADE
Sbjct: 181 TWEDNTKFSFFLAPRVQTADILVSFERGDHGDYQAFDGEGGILAHSLGAVDGRVHFDADE 240

Query: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300
            W E G +  +  D ESVALHE+GH LGLGHS  PSAVM+A M+ GV KT LTIDDIEG+
Sbjct: 241 PW-ESGPI-EDRVDLESVALHELGHTLGLGHSREPSAVMYAFMDPGVYKTHLTIDDIEGI 300

Query: 301 HAL 304
            AL
Sbjct: 301 CAL 301

BLAST of Csa5G632070 vs. NCBI nr
Match: gi|659118031|ref|XP_008458914.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 438.3 bits (1126), Expect = 1.1e-119
Identity = 217/303 (71.62%), Postives = 245/303 (80.86%), Query Frame = 1

Query: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60
           MA+KLCLQLFLLLAF+TPHLTSAR LHNHK SRLLF EHLIGSSKND+IVEGIHKVKAYL
Sbjct: 1   MASKLCLQLFLLLAFITPHLTSARHLHNHKPSRLLFSEHLIGSSKNDNIVEGIHKVKAYL 60

Query: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120
           QRYGYF+NEN+NNLSTDAFDD LESAIK YQKFSNLKVSGVLDRETLQQMS+PRCGV D 
Sbjct: 61  QRYGYFNNENDNNLSTDAFDDRLESAIKLYQKFSNLKVSGVLDRETLQQMSKPRCGVRDT 120

Query: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180
           F S+AQQ+ EN NT ++IGGS + FF     WP  + HL+YGF+HNYP +    ++RAF 
Sbjct: 121 FASIAQQEHENKNTNIEIGGSRYTFFFNNVSWPAERKHLSYGFIHNYPPQFVDPLLRAFQ 180

Query: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240
            W  N+KF+F LA R+QTADIL+SFERG HGD + FDGEGGIL H+ G IDGRVHFDADE
Sbjct: 181 TWEDNTKFSFFLAPRVQTADILVSFERGAHGDYQAFDGEGGILAHSLGAIDGRVHFDADE 240

Query: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300
            W  I     +  D ESVALHE+GH LGLGHS   SAVM+A ME G+ KT LTIDDIEG+
Sbjct: 241 AWERIP--IEQRVDLESVALHELGHTLGLGHSSEISAVMFAYMEPGLYKTRLTIDDIEGI 300

Query: 301 HAL 304
            AL
Sbjct: 301 RAL 301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
2MMP_ARATH3.4e-4940.30Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
5MMP_ARATH5.8e-4942.21Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1[more]
3MMP_ARATH1.9e-4738.29Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
MEP1_SOYBN2.3e-4542.31Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
1MMP_ARATH2.5e-4436.48Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KS35_CUCSA3.2e-179100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1[more]
A0A0A0KVC8_CUCSA4.1e-11869.64Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632060 PE=3 SV=1[more]
A0A0A0KU02_CUCSA1.9e-7048.71Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1[more]
A0A0A0KS39_CUCSA1.3e-6848.38Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1[more]
A0A0A0KNY8_CUCSA2.5e-6746.75Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G70170.11.9e-5040.30 matrix metalloproteinase[more]
AT1G59970.13.3e-5042.21 Matrixin family protein[more]
AT1G24140.11.1e-4838.29 Matrixin family protein[more]
AT4G16640.11.4e-4536.48 Matrixin family protein[more]
AT2G45040.13.2e-3735.17 Matrixin family protein[more]
Match NameE-valueIdentityDescription
gi|778708735|ref|XP_004142466.2|4.6e-179100.00PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|659118025|ref|XP_008458911.1|8.2e-13679.08PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|659118027|ref|XP_008458912.1|5.5e-12471.57PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|659118029|ref|XP_008458913.1|1.0e-12273.60PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|659118031|ref|XP_008458914.1|1.1e-11971.62PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001818Pept_M10_metallopeptidase
IPR002477Peptidoglycan-bd-like
IPR006026Peptidase_Metallo
IPR016293Peptidase M10A, stromelysin-type
IPR021190Pept_M10A
IPR024079MetalloPept_cat_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
GO:0005509calcium ion binding
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa5G632070.1Csa5G632070.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 156..303
score: 1.7
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 52..111
score: 1.7
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 52..110
score: 6.8
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 51..119
score: 9.16
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 148..306
score: 3.5
IPR016293Peptidase M10A, stromelysin-typePIRPIRSF001191MMP_stromelysincoord: 21..304
score: 1.1
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 258..283
score: 3.2E-31coord: 199..227
score: 3.2E-31coord: 110..123
score: 3.2E-31coord: 175..190
score: 3.2E-31coord: 292..305
score: 3.2
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 112..303
score: 7.8
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 1..21
score: 1.2E-102coord: 37..303
score: 1.2E
NoneNo IPR availablePANTHERPTHR10201:SF152SUBFAMILY NOT NAMEDcoord: 1..21
score: 1.2E-102coord: 37..303
score: 1.2E
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 145..303
score: 6.11