Csa5G523060 (gene) Cucumber (Chinese Long) v2

BLAST of Csa5G523060 vs. Swiss-Prot
Match: GPAA1_MOUSE (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus GN=Gpaa1 PE=1 SV=3)

HSP 1 Score: 124.4 bits (311), Expect = 5.0e-27
Identity = 173/721 (23.99%), Postives = 287/721 (39.81%), Query Frame = 1

Query: 10  KARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPGSANSMLSGR 69
           + R + R+ + L +     S V   A  LAL   P+    TY+SENA+          G 
Sbjct: 9   RRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPL-TQRTYMSENAMGSTMVEEQFVGG 68

Query: 70  EVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADV---NYHRFHPQSSQFHPLH 129
           +   A +  +D      KPG+        L + +  +G +V   ++ R  P   + H  +
Sbjct: 69  D--RARSFARDFAAHRKKPGALPVAW---LERSMRSVGLEVYTQSFSRKLPFPDETHERY 128

Query: 130 FFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDTLSLGIA 189
                           M  G N  GI+RAP++   E++VL  P  P  T+   + ++G+ 
Sbjct: 129 ----------------MVSGTNVYGILRAPRSASTESLVLTVPCGPDATN---SQAVGLL 188

Query: 190 YSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHT-PVFG-QSSVIDTDACSET 249
            ++ +      + AKDI++LV D    +     AWL  YH   V G QSS +        
Sbjct: 189 LALAAHFRGQIYWAKDIIFLVTDH---DLLGTEAWLEAYHDINVTGIQSSPLQG------ 248

Query: 250 NVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLD 309
                                RAG + AA+ +++S+       SL V  E  NGQ+PNLD
Sbjct: 249 ---------------------RAGAIQAAVALELSS---DVVTSLDVTVEGLNGQLPNLD 308

Query: 310 LINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGMSASD 369
           L+N+          F+   +K   L   +            GK+      +W    S   
Sbjct: 309 LLNL----------FQTFCQKGGLLCTLQ------------GKL---QPQDWT---SLEG 368

Query: 370 YVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFILRGGR 429
            + G  TL   +  QA G P G HG F  Y ++A+T+     F    + + D  +   G+
Sbjct: 369 PLQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSFR---QYKYD--LATVGK 428

Query: 430 LIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALYSYAN 489
            +EG+ R +N+LLE+ HQSFF YL+ +  +FVS+G+YM A   L+  L   A  L+   +
Sbjct: 429 ALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLH 488

Query: 490 NLNLTSEKV-EPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQIPG 549
              +  E+  + P+   P    ++  +     +    + +    G  +  LP     +  
Sbjct: 489 QAGVNPEEAGKAPSPGTP----LLPTQGVGLASLTAPLLISQAMGLALYFLPVLGQHLAT 548

Query: 550 YSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQE--WAFLKAMTTSAAFIGLC 609
                      +L+LL++ V  + L  P ++ +    ++ +  W  LK +      + L 
Sbjct: 549 QHFPVAEAEAVVLTLLAIYVAGLAL--PHNTHRVVNSQVPDRGWMALKLVALIYLALQLG 608

Query: 610 LMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVT--- 669
            ++++NFS   L A  +V    LA P     G     A         L  +  P VT   
Sbjct: 609 CIALLNFSLGFLLAATMVPAAALAKP----HGPRTLYA--------ALLVVTSPAVTLFG 618

Query: 670 -FFLFKGALQGFDNLHIGDFWNWMETL--------WAWNSATFLYLGMVHLPCWLLCTQI 711
             FL++  L+    L + + W    T         + + +  F  L +   PCWLL   +
Sbjct: 669 SLFLWRELLE--VPLSLAEGWQLFLTALAQGVLEHYTYGALLFPILALGLYPCWLLFWNV 618

BLAST of Csa5G523060 vs. Swiss-Prot
Match: GPAA1_HUMAN (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens GN=GPAA1 PE=1 SV=3)

HSP 1 Score: 124.4 bits (311), Expect = 5.0e-27
Identity = 158/629 (25.12%), Postives = 261/629 (41.49%), Query Frame = 1

Query: 10  KARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPGSANSMLSGR 69
           + R + RL + L +     S V   A  LAL+  P+    TY+SENA+         +G 
Sbjct: 9   RRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPL-TQRTYMSENAMGSTMVEEQFAGG 68

Query: 70  EVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSSQFHPLHFFS 129
           + + A    +D      K G+       +   ++ +    V    +    S+  P     
Sbjct: 69  DRARA--FARDFAAHRKKSGA-------LPVAWLERTMRSVGLEVYTQSFSRKLPF---- 128

Query: 130 SPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDTLSLGIAYSI 189
            PD    + +   M  G N  GI+RAP+A   E++VL  P     T+   + ++G+  ++
Sbjct: 129 -PD----ETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTN---SQAVGLLLAL 188

Query: 190 FSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYH-TPVFG-QSSVIDTDACSETNVL 249
            +      + AKDIV+LV +    +     AWL  YH   V G QSS +           
Sbjct: 189 AAHFRGQIYWAKDIVFLVTEH---DLLGTEAWLEAYHDVNVTGMQSSPLQG--------- 248

Query: 250 DEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLIN 309
                             RAG + AA+ +++S+       SL V  E  NGQ+PNLDL+N
Sbjct: 249 ------------------RAGAIQAAVALELSS---DVVTSLDVAVEGLNGQLPNLDLLN 308

Query: 310 IVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGMSASDYVD 369
           +          F+   +K   L   +            GK+      +W    S    + 
Sbjct: 309 L----------FQTFCQKGGLLCTLQ------------GKL---QPEDWT---SLDGPLQ 368

Query: 370 GTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFILRGGRLIE 429
           G  TL   +  QA G P GSHG F  Y+++A+T+     F    + + D  ++  G+ +E
Sbjct: 369 GLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFR---QYKYD--LVAVGKALE 428

Query: 430 GVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYM--IAFALLVAPLPAVAAALYSYANN 489
           G+ R +N+LLE+ HQSFFLYL+    +FVS+G+YM  + F LLV  L A+   +  +   
Sbjct: 429 GMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAG 488

Query: 490 LNLTSEKVEP-PALANPDDE------LIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFI 549
           + L      P P++  P  +      L+  L   + +  A  ++V+ + G  V+   + +
Sbjct: 489 MGLEEPGGAPGPSVPLPPSQGVGLASLVAPLLISQAMGLA--LYVLPVLGQHVATQHFPV 537

Query: 550 CQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFI 609
            +              +L+LL++    + L        S +   + W  LK +      +
Sbjct: 549 AEAEAV----------VLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLAL 537

Query: 610 GLCLMSVINFSTAELGAFLVVSMCLLAHP 628
            L  +++ NFS   L A  +V    LA P
Sbjct: 609 QLGCIALTNFSLGFLLATTMVPTAALAKP 537

BLAST of Csa5G523060 vs. Swiss-Prot
Match: GAA1_SCHPO (GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=gaa1 PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.2e-17
Identity = 109/467 (23.34%), Postives = 182/467 (38.97%), Query Frame = 1

Query: 15  VRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAM--NTYISENALMPGSANSMLSGREVS 74
           +R+  FL  H  F  +     G+  + +LP   +    ++SE+AL+PG  N+    R   
Sbjct: 9   IRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRY-- 68

Query: 75  EANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSSQFHPLHFFSSPD 134
                            S    S    A   S L A V  +  +    Q      F++  
Sbjct: 69  -----------------SKTVSSSLTAANTWSHLDASVGTNTMYDDLEQI-----FTAMG 128

Query: 135 SGILKDNVSCMT-----QGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDTLSLGIAY 194
               K N S         G N +  +RAP+ D  E+++L  P+       ++   + +A 
Sbjct: 129 LPTQKQNYSINIPGSEFNGSNFITTLRAPRGDATESLLLCVPWKD-HIGQYNEAGVALAI 188

Query: 195 SIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDACSETNVL 254
           S+       +  +KDI+ ++ D                  PV+G SS + T    +T   
Sbjct: 189 SLLKYFQGWSLWSKDIILVIFDD-----------------PVYGPSSFL-TSYFDQTTPY 248

Query: 255 DEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPN-LDLI 314
             +   ++          R+G++ A L +++   +E+  D L V  +A+NGQ+   LDL 
Sbjct: 249 ISYTPLKI----------RSGSIQAGLSLELVT-TENNSDVLEVLYQATNGQL-PNLDLF 308

Query: 315 NIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGMSASDYV 374
           N ++ + +    + ++++ +    N                             S S Y 
Sbjct: 309 NTISRIFMQHFNYPLRLQGYDFHAN-----------------------------SGSSYT 368

Query: 375 DGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDF---ILRGG 434
               +L   +  QAV   T +H  F  Y+ID +T+ M  K         D F   + R G
Sbjct: 369 SRLKSLWMGMLTQAVSNVTSAHALFPQYRIDMLTLRMKVK---------DPFSFDMFRFG 382

Query: 435 RLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVA 471
           + IE   RS+NNLLE  HQSFF Y ++    F+S+G YM +  +L A
Sbjct: 429 QAIESTFRSLNNLLEHLHQSFFFYFILDHLHFISIGNYMPSILILAA 382

BLAST of Csa5G523060 vs. Swiss-Prot
Match: GAA1_YEAST (GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GAA1 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 7.5e-15
Identity = 175/747 (23.43%), Postives = 285/747 (38.15%), Query Frame = 1

Query: 1   MAETEKLKPKARPIVRLGIF--LISHSIFFSVVCFSAGVLALLLLPMFAM--NTYISENA 60
           MA  EKL    R IV +G+   +I+     S++C   G +++ +LPM      TYISENA
Sbjct: 1   MALLEKLH---RRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENA 60

Query: 61  LMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFH 120
           LMP  A S    RE SE N L      +      +      ++  ++ + G     +   
Sbjct: 61  LMPSQAYSYF--RE-SEWNILRGYRSQIKEMVNMTSMERNNLMGSWLQEFGTKTAIY--- 120

Query: 121 PQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETS 180
            ++ Q+                       G    G++ AP+ DG EA+VL          
Sbjct: 121 -ENEQY-----------------------GETLYGVMHAPRGDGTEAMVL---------- 180

Query: 181 LHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAA--VSAWLRDYHTPVFGQSS 240
                              V W   D  + +  +  G   A   S W      PV+ ++ 
Sbjct: 181 ------------------AVPWFNSDDEFNIGGAALGVSLARFFSRW------PVWSKNI 240

Query: 241 VIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAE 300
           ++         +    EA   +    LD     G++ AA+V+  S+  + FE  + +  +
Sbjct: 241 IVVFSENPRAALRSWVEAYHTS----LD--LTGGSIEAAVVLDYSSTEDFFE-YVEISYD 300

Query: 301 ASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNS 360
             NG++PNLDL+NI   +  H +G ++       L      ++    F S  K++     
Sbjct: 301 GLNGELPNLDLVNIAISITEH-EGMKV------SLHGLPSDQLTNNNFWSRLKILCLGIR 360

Query: 361 EWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVR 420
           +W                        V  P G+  AF  ++I ++T++        I   
Sbjct: 361 DWALS--------------------GVKKPHGNE-AFSGWRIQSVTLKAHGNSGHDITTF 420

Query: 421 RDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYM-IAFALLVAPLP 480
                   GR+ E + RS+NNLLEKFHQSFF YL+++  +FVS+  Y+  A AL +A   
Sbjct: 421 --------GRIPEAMFRSINNLLEKFHQSFFFYLLLAPRQFVSISSYLPSAVALSIA--- 480

Query: 481 AVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSL 540
              ++L ++ NN           A AN      +SL S ++   A  V+ V L      +
Sbjct: 481 FAISSLNAFINN-----------AYAN------ISLFS-EYNLVALLVWFVSL------V 540

Query: 541 LPYFICQIPGYSPTENSIIWGLLSLLSL--LVLSVILGSPFSSTKSYEQRIQEWAFLKAM 600
           + + + Q     P+      GLL  +S+    L +IL      ++    R++  AFL   
Sbjct: 541 ISFVVSQAFLLIPSS-----GLLMTISMASCFLPLILSRKIHISEPLSYRLKNVAFLYFS 594

Query: 601 TTSAAFIGLCLMSVINFSTAELGAFLVVSMCLL----------------AHPLK------ 660
             S + +      +INF+ A L   L   M  +                ++P+K      
Sbjct: 601 LVSTSLL------MINFAMALLIGTLAFPMTFVKTIVESSSEHEVTTQSSNPIKTEPKDE 594

Query: 661 -------LDLGAGNFKALSRAACNLVLGFIAFPPVTFFLFKGALQGFDNLHIGDFWNWME 706
                  +D      +   +   NLVL  +  P ++  LF   L   D  H  D  N + 
Sbjct: 661 IELVENHMDTTPATPQQQKQKLKNLVLLILTNPFISITLF--GLFFDDEFHGFDIINKLV 594

BLAST of Csa5G523060 vs. TrEMBL
Match: M5WSJ7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002024mg PE=4 SV=1)

HSP 1 Score: 983.4 bits (2541), Expect = 1.5e-283
Identity = 470/710 (66.20%), Postives = 584/710 (82.25%), Query Frame = 1

Query: 6   KLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPGSANSM 65
           + +PK RPIV+LGIFLISHS+ FSVV  +AGVLALLLLP+ A NTYISENALMPGS   M
Sbjct: 18  RARPKPRPIVQLGIFLISHSLLFSVVFSTAGVLALLLLPVLAKNTYISENALMPGSVAPM 77

Query: 66  LSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSSQFHPL 125
           LS +EV+EAN LVKDL  LNSKP +S  GSQ++++QY+S LGA+V++H+FHPQ +QFHPL
Sbjct: 78  LSNQEVAEANRLVKDLTSLNSKPLASAIGSQRLISQYMSNLGAEVSHHKFHPQLNQFHPL 137

Query: 126 HFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDTLSLGI 185
           HFFSSPDSG ++ N+SC   G+NTVGIIRAP+ DGKEAIVLVTP+N V+ + ++ LSL I
Sbjct: 138 HFFSSPDSGRVEQNISCALHGVNTVGIIRAPRGDGKEAIVLVTPFNSVKLNQNEALSLAI 197

Query: 186 AYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDACSETN 245
           AYS+FSLLT+V WLAKD++WLVADS++GEY+ V+AWLRDYHTPVF     +D + C E+N
Sbjct: 198 AYSVFSLLTRVPWLAKDVIWLVADSQFGEYSGVAAWLRDYHTPVFSGLGTLDAEMCPESN 257

Query: 246 VLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDL 305
            +     N  T++ I D FKRAGTMAAALVIKV++RSE +EDSLS+YAEASNGQMPNLDL
Sbjct: 258 NIYGLNENLSTKRTIYDGFKRAGTMAAALVIKVADRSEQYEDSLSIYAEASNGQMPNLDL 317

Query: 306 INIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGMSASDY 365
           INIVNYLAVHRQG R+K+EKFWPLL  KWLK LGE+FES+G + RS+N +WKFG+ A+DY
Sbjct: 318 INIVNYLAVHRQGLRVKVEKFWPLLGLKWLKTLGEIFESLGHLARSVNPQWKFGIPAADY 377

Query: 366 VDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSS-GIKVRRDDFILRGGR 425
           ++G+ATLASSLYYQA+G+PTG HGAFRDYQ+DAIT+E+ PK SS   KVR++DF+LR GR
Sbjct: 378 IEGSATLASSLYYQALGVPTGPHGAFRDYQVDAITLEIFPKVSSLDNKVRKNDFLLRSGR 437

Query: 426 LIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALYSYAN 485
           LIEGV+RSVNNLLEKFHQSFFLYL+ S GKFVSVGVYMIAFALLVAPLP VAA+LY  A+
Sbjct: 438 LIEGVIRSVNNLLEKFHQSFFLYLLTSPGKFVSVGVYMIAFALLVAPLPMVAASLYVDAS 497

Query: 486 NLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQIPGY 545
           N + + +  +P   AN  DE  ++L+SWKWL AAK+VF VHLWG++VSLLPYFICQIP  
Sbjct: 498 NSDYSLKIDKPAPFANASDEPCITLKSWKWLFAAKKVFFVHLWGSVVSLLPYFICQIPSC 557

Query: 546 SPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMS 605
           +PT + IIW  LS+LSL+V+ ++L SP S     + +  EWA LK++T SAAFIGLCLMS
Sbjct: 558 TPTTSFIIWVFLSILSLVVMYIVLASPSSHANESQPQRGEWAILKSVTLSAAFIGLCLMS 617

Query: 606 VINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFFLFKG 665
           VINF++AE+G  L+V MCL+AHPLKLD+ A + + +SR   NLVLGFI FPP+ F + KG
Sbjct: 618 VINFASAEIGGLLMVPMCLMAHPLKLDVKARSIRTVSRVVYNLVLGFIGFPPIAFIVLKG 677

Query: 666 ALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC 715
           A +GF  +++GDFW+W+E+LWAWNSAT+LY+GMV+LPCW+LC  IL H C
Sbjct: 678 AFEGFSGVNVGDFWSWVESLWAWNSATYLYIGMVYLPCWVLCIHILFHRC 727

BLAST of Csa5G523060 vs. TrEMBL
Match: A0A061EBY2_THECC (GPI transamidase component family protein / Gaa1-like family protein OS=Theobroma cacao GN=TCM_016964 PE=4 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 3.0e-273
Identity = 462/721 (64.08%), Postives = 580/721 (80.44%), Query Frame = 1

Query: 1   MAET-------EKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYIS 60
           MAET       EK KP+ RPIVRLGIFLISHS+  SVVC +AGVLAL LLP+ A NTYIS
Sbjct: 1   MAETKETSEKDEKPKPRVRPIVRLGIFLISHSVLVSVVCCTAGVLALFLLPVLAKNTYIS 60

Query: 61  ENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYH 120
           ENALMPGSA+ MLS + VS+ N LVKDL  L+ K   +   SQ+++AQY+ +LGA+V++H
Sbjct: 61  ENALMPGSASPMLSYQHVSDGNRLVKDLTNLDLKSSETGIESQRVIAQYMLELGAEVSFH 120

Query: 121 RFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPV 180
           +FH Q +QFHPLHFFSSPDSG++++N SC + GINTVGIIRAP+ DGKEAIVLVTPYN +
Sbjct: 121 KFHAQLNQFHPLHFFSSPDSGVIQENFSCSSYGINTVGIIRAPRGDGKEAIVLVTPYNAL 180

Query: 181 ETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQS 240
           ++ L + +S+GIAYS+FSLLTQVTWLAKD++WLVADSRYGEYAAV+AWLRDY  P F   
Sbjct: 181 KSGLGEAVSVGIAYSVFSLLTQVTWLAKDVIWLVADSRYGEYAAVAAWLRDYQIPKFSSL 240

Query: 241 SVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYA 300
           S ++++ C + N L + + N +   +  + F+RAGTMAAALV+KV ++SE +ED++S+YA
Sbjct: 241 STLNSEMCPDINNLYDLKVNSIAGSKYSNSFRRAGTMAAALVLKVGDQSEQYEDTISIYA 300

Query: 301 EASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLN 360
           EASNGQMPNLDLIN+VNYLAVHRQG R+++EK W LLN  WLK LGE+FES+GKV +SLN
Sbjct: 301 EASNGQMPNLDLINVVNYLAVHRQGLRVQVEKLWSLLNSSWLKSLGEIFESLGKVAKSLN 360

Query: 361 SEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKV 420
            EWKFG+ A++YV+GTATLASSLYYQA+G+PTG HGAFRDYQ+DAIT+E+SPK+S   K 
Sbjct: 361 PEWKFGIPATEYVEGTATLASSLYYQALGVPTGPHGAFRDYQVDAITLEISPKYSLD-KF 420

Query: 421 RRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLP 480
           RR+D +LRGGRLIEGV+RSVNNLLEKFHQSFFLYL+ S  KFVSVGVYMIA ALL+APLP
Sbjct: 421 RRNDILLRGGRLIEGVIRSVNNLLEKFHQSFFLYLLTSPSKFVSVGVYMIALALLIAPLP 480

Query: 481 AVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSL 540
            VAA+LY  AN+L+  + K  P  +A+   E  +++RSW+WL +AK VF VH+WGAIVSL
Sbjct: 481 MVAASLYVDANSLDSKTNKSTPSTIAD-TAERGITIRSWRWLYSAKLVFAVHVWGAIVSL 540

Query: 541 LPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTT 600
           LPYFICQIP  S T + +IW +LS++SLL L  +L SPFS   + +++  EWA +K++T 
Sbjct: 541 LPYFICQIPDCSSTISFMIWVVLSMISLLTLYSVLASPFSCNNASQEK--EWALVKSVTI 600

Query: 601 SAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIA 660
           S  FIGL LMSVINF+TAE+GA L+V MCLLA PLK DL AG+ ++ SR  CNLVLG IA
Sbjct: 601 SYVFIGLGLMSVINFATAEIGALLMVPMCLLARPLKFDLRAGSLRSFSRMVCNLVLGLIA 660

Query: 661 FPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHP 715
           FPP  FFL KG L+ F +++IGDFW W+E+LWAWNSAT++++GMV+LPCW+LC  ILLH 
Sbjct: 661 FPPAAFFLLKGMLEDFGSVNIGDFWMWVESLWAWNSATYIFIGMVYLPCWVLCVHILLHT 717

BLAST of Csa5G523060 vs. TrEMBL
Match: A0A0D2RYU2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_012G064300 PE=4 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 3.4e-272
Identity = 466/722 (64.54%), Postives = 572/722 (79.22%), Query Frame = 1

Query: 1   MAET-------EKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYIS 60
           MAET       EK KP+ RPIVRLGIFLISHS   SVVC +AGVLAL LLP+ A NTYIS
Sbjct: 1   MAETKEKSLSDEKPKPRVRPIVRLGIFLISHSFHVSVVCCTAGVLALFLLPVLAKNTYIS 60

Query: 61  ENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYH 120
           ENALMPGSA+ MLS + VS+ N LVKDL   NSK   +   S +I+AQY+  LGA+V++H
Sbjct: 61  ENALMPGSASPMLSNQHVSDGNRLVKDLTNPNSKSSETGIESHKIIAQYMLDLGAEVSFH 120

Query: 121 RFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPV 180
           +FHPQ +QFHPLHFFSSPDSG +++N SC + GINTVGIIRAP+ DGKEAIVLVTPYN +
Sbjct: 121 KFHPQMNQFHPLHFFSSPDSGKIQENYSCSSYGINTVGIIRAPRGDGKEAIVLVTPYNAL 180

Query: 181 ETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQS 240
           ++   + LSLGIAYS+FSLLTQVTWLAKDI+WLVADS+YGEYAAV+AWLR+Y TP F   
Sbjct: 181 KSGFGEALSLGIAYSVFSLLTQVTWLAKDIIWLVADSQYGEYAAVAAWLREYQTPKFSSL 240

Query: 241 SVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYA 300
           S  + + C + N L E + + ++  +  + F+RAGTMAAALV+KV +++E FED++S+YA
Sbjct: 241 SAPNAEMCPDVNNLFELKGHSISGSKFSNSFRRAGTMAAALVLKVRDQNEQFEDTISIYA 300

Query: 301 EASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLN 360
           EASNGQMPNLDLIN+VNYLAVHRQG R+ +EK W LLN  WLK LGE+FES+ KV +SL 
Sbjct: 301 EASNGQMPNLDLINVVNYLAVHRQGLRVNVEKLWSLLNSSWLKSLGEIFESLAKVAKSLY 360

Query: 361 SEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKV 420
            +WKFG+ A++YV+GTATLASSLYYQA+G+PTG HGAFRDYQ+DAIT+E+SPKFS   KV
Sbjct: 361 PKWKFGIPATEYVEGTATLASSLYYQALGVPTGPHGAFRDYQVDAITLEISPKFSLD-KV 420

Query: 421 RRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLP 480
            R+D +LRGGRLIEGV+RSVNNLLEKFHQSFFLYL+ S  KFVSVGVYMIAFALL+APLP
Sbjct: 421 WRNDILLRGGRLIEGVIRSVNNLLEKFHQSFFLYLLTSPSKFVSVGVYMIAFALLIAPLP 480

Query: 481 AVAAALYSYANNLNLTSE-KVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVS 540
            VAA+LY  AN+ +  S+     P+     DE  +++RSW+WLN+AK VF+VHLWGAIVS
Sbjct: 481 MVAASLYVDANSSSKRSKGDSSTPSAITDTDEHSIAIRSWRWLNSAKLVFIVHLWGAIVS 540

Query: 541 LLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMT 600
           LLPYFI QIP  SPT + +IW LLS+LSLL L+ +L SP +  K+ +++  EWA +K++T
Sbjct: 541 LLPYFISQIPDCSPTTSFMIWVLLSILSLLTLNSVLASPLACAKATQEK--EWALVKSVT 600

Query: 601 TSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFI 660
            S+ FIGL LMSVINF+TAE+GA L+V MCLLA PLKLD+ AG  ++  R  CNLVLG I
Sbjct: 601 ISSVFIGLGLMSVINFATAEIGALLMVPMCLLAQPLKLDVRAGTLRSFCRMICNLVLGHI 660

Query: 661 AFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLH 715
           AFPP  FFL KG L GF N ++GDFW W+E+LWAWNSAT+L++GMV LPCW+LC  IL H
Sbjct: 661 AFPPAAFFLLKGMLDGFGNANVGDFWMWVESLWAWNSATYLFIGMVQLPCWVLCISILFH 719

BLAST of Csa5G523060 vs. TrEMBL
Match: A0A0B0NDV8_GOSAR (Glycosylphosphatidylinositol anchor attachment 1 OS=Gossypium arboreum GN=F383_19336 PE=4 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 2.8e-271
Identity = 465/722 (64.40%), Postives = 569/722 (78.81%), Query Frame = 1

Query: 1   MAET-------EKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYIS 60
           MAET       EK KP+ RPIVRLGIFLISHS   SVVC +AGVLAL LLP+ A NTYIS
Sbjct: 1   MAETKEKSLSDEKPKPRVRPIVRLGIFLISHSFLVSVVCCTAGVLALFLLPVLAKNTYIS 60

Query: 61  ENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYH 120
           ENALMPGSA+ MLS + VS+ N LVKDL   NSK   +   S +I+AQY+  LGA+V++H
Sbjct: 61  ENALMPGSASPMLSNQHVSDGNRLVKDLTNPNSKSSETGIESHKIIAQYMLDLGAEVSFH 120

Query: 121 RFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPV 180
           +FHPQ +QFHPLHFFSSP SG +++N SC + GINTVGIIRAP+ DGKEAIVLVTPYN +
Sbjct: 121 KFHPQMNQFHPLHFFSSPVSGKIQENYSCSSYGINTVGIIRAPRGDGKEAIVLVTPYNAL 180

Query: 181 ETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQS 240
           ++   + LSLGIAYS+FSLLTQVTWLAKDI+WLVADS+YGEYAAV+AWLR+Y TP F   
Sbjct: 181 KSGFGEALSLGIAYSVFSLLTQVTWLAKDIIWLVADSQYGEYAAVAAWLREYQTPKFSSL 240

Query: 241 SVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYA 300
           S  + + C + N L E + + ++  +  + F+RAGTMAAALV+KV +++E FED++S+YA
Sbjct: 241 STPNAEMCPDVNNLFELKGHSISGSKFSNSFRRAGTMAAALVLKVRDQNEQFEDTISIYA 300

Query: 301 EASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLN 360
           EASNGQMPNLDLIN+VNYLAVHRQG R+ +EK WPLLN  WLK LGE+FES+ KV +SL 
Sbjct: 301 EASNGQMPNLDLINVVNYLAVHRQGLRVNVEKLWPLLNSSWLKSLGEIFESLAKVAKSLY 360

Query: 361 SEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKV 420
            +WKFG+ A++YV+GTATLASSLYYQA+G+PTG HGAFRDYQ+DAIT+E+SPKFS   KV
Sbjct: 361 PKWKFGIPATEYVEGTATLASSLYYQALGVPTGPHGAFRDYQVDAITLEISPKFSLD-KV 420

Query: 421 RRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLP 480
            R+D +LRGGRLIEGV+RSVNNLLEKFHQSFFLYL+ S  KFVSVGVYMIAFALL+A LP
Sbjct: 421 WRNDILLRGGRLIEGVIRSVNNLLEKFHQSFFLYLLTSPSKFVSVGVYMIAFALLIASLP 480

Query: 481 AVAAALYSYAN-NLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVS 540
            VAA+LY  AN + N +      P+     DE  +++RSW+WLN+AK VF+VHLWGAIVS
Sbjct: 481 MVAASLYVDANSSSNCSKGDSSTPSAITDTDEHSIAIRSWRWLNSAKLVFIVHLWGAIVS 540

Query: 541 LLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMT 600
           LLPYFI QIP  SPT + +IW LLS+LSLL L+ +L SP +  K+ +++  EWA +K++T
Sbjct: 541 LLPYFISQIPDCSPTTSFMIWVLLSILSLLTLNSVLASPLACAKATQEK--EWALVKSVT 600

Query: 601 TSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFI 660
            S  FIGL LMSVINF+TAE+GA L+V MCLLA PLKLD+ AG  ++  R  CNLVLG I
Sbjct: 601 ISLVFIGLGLMSVINFATAEIGALLMVPMCLLARPLKLDVRAGTLRSFCRLICNLVLGHI 660

Query: 661 AFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLH 715
           AFPP  FFL KG L GF N ++GDFW W+E+LWAWNSAT+L++GMV LPCW+LC  IL H
Sbjct: 661 AFPPAAFFLLKGMLDGFGNANVGDFWMWVESLWAWNSATYLFIGMVQLPCWVLCVSILFH 719

BLAST of Csa5G523060 vs. TrEMBL
Match: B9GI62_POPTR (GPI transamidase component family protein OS=Populus trichocarpa GN=POPTR_0001s35320g PE=4 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 4.1e-270
Identity = 453/713 (63.53%), Postives = 567/713 (79.52%), Query Frame = 1

Query: 3   ETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPGSA 62
           ET+  K K RPIVRLGIFLISHS+  SVVC +AGVLALLLLP+ A +TYISENALMPGSA
Sbjct: 5   ETDTPKMKPRPIVRLGIFLISHSLLVSVVCCTAGVLALLLLPLLAKSTYISENALMPGSA 64

Query: 63  NSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSSQF 122
           +SM+S R++SEAN L  DL GLN +P  +    + IL +Y+S LGA+V+YH+FH + +QF
Sbjct: 65  SSMISNRDISEANRLASDLSGLNFEPQDAA-AFRGILTKYMSDLGAEVSYHKFHHKPNQF 124

Query: 123 HPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDTLS 182
           HPLHFFSSPDS     N SC   G+NTVGIIRAP+ DGKEAIVLVTPY   ++   +TLS
Sbjct: 125 HPLHFFSSPDSTSSVKNFSCSAHGVNTVGIIRAPRGDGKEAIVLVTPYKFGKSGAAETLS 184

Query: 183 LGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDACS 242
           L IAY++FSLL++VTWLAKDI+W VADS+YGEYA V AWLRDYHTPVF     ++ D C 
Sbjct: 185 LSIAYTVFSLLSRVTWLAKDIIWFVADSQYGEYAPVEAWLRDYHTPVFTGLGTLNADTCV 244

Query: 243 ETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPN 302
           + + L  FE   + +++I D F+RAGTMAAAL++KV++R+E  ED+L++YAEASNGQMPN
Sbjct: 245 KIDDLYVFEQKSIVQRKISDGFRRAGTMAAALILKVADRNELLEDTLNIYAEASNGQMPN 304

Query: 303 LDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGMSA 362
           LDLINIVNYLAVHRQG R+K+EK   LL+ +WL++LGE+FE +GKV RSLN  WKFG+SA
Sbjct: 305 LDLINIVNYLAVHRQGLRVKVEKVLTLLDLRWLEILGEMFELLGKVARSLNPGWKFGISA 364

Query: 363 SDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFILRG 422
           +DY++G+ATLASSLYYQA+GIPTG HGAFRD+Q+DAIT+E+SPK     K R ++F+LRG
Sbjct: 365 ADYIEGSATLASSLYYQALGIPTGPHGAFRDFQVDAITLEISPKVFPHNKARHNEFLLRG 424

Query: 423 GRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALYSY 482
           GRL+EGV+RS+NNLLEKFHQSFFLY++ S  KFVSVGVYMIAFALLVAPLP VAA+LY+ 
Sbjct: 425 GRLVEGVIRSINNLLEKFHQSFFLYMLTSPSKFVSVGVYMIAFALLVAPLPLVAASLYAD 484

Query: 483 ANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQIP 542
           AN L+L  +  +    A   DEL ++ RSWKWL AAK VF++H+WGA VSLLPYFICQIP
Sbjct: 485 ANQLDLGVKNTKSTPTATASDELDITFRSWKWLYAAKEVFLIHVWGAAVSLLPYFICQIP 544

Query: 543 GYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTK-SYEQRIQEWAFLKAMTTSAAFIGLC 602
             SPT +S+IW  LS+LSLL+L +I+GSP +    S  +   EWA LK+ T SA FIGL 
Sbjct: 545 NCSPTSSSVIWVFLSMLSLLILYLIVGSPSTHVSISQPEEKGEWAILKSATISAVFIGLL 604

Query: 603 LMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFFL 662
           +MSV+NF+ AE+GA L+V MCL+A PLKLD+ AG  ++  R  CN+VLGF+AFPP  FF+
Sbjct: 605 VMSVVNFAAAEIGALLLVPMCLMAQPLKLDMKAGKLRSFLRIICNVVLGFVAFPPAAFFV 664

Query: 663 FKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC 715
            K   +GFD++++GDFWNWME+LWAWNSAT++Y+GMVHLPCW+LC  ILLH C
Sbjct: 665 VKTIFEGFDSINMGDFWNWMESLWAWNSATYIYIGMVHLPCWVLCLHILLHSC 716

BLAST of Csa5G523060 vs. TAIR10
Match: AT5G19130.1 (AT5G19130.1 GPI transamidase component family protein / Gaa1-like family protein)

HSP 1 Score: 808.9 bits (2088), Expect = 2.5e-234
Identity = 415/711 (58.37%), Postives = 520/711 (73.14%), Query Frame = 1

Query: 8   KPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPGSANSMLS 67
           K K RPIVRLGIFLI+HS  FSVV  +AGVLALLLLP+ A NTYISENALMPGSA SMLS
Sbjct: 20  KIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSMLS 79

Query: 68  GREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSSQFHPLHF 127
            R+VS+ + LVKD+K             Q+++ +Y+S +GA+V+Y +FHP+ +QFHPLHF
Sbjct: 80  NRDVSDGSKLVKDIKNFRLNHEGQGVEVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHF 139

Query: 128 FSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDTLSLGIAY 187
           FS PDS  L +NVSC + G+N  GIIRAP+ DGKE+IVLVTPY+ +    ++ LSLGI  
Sbjct: 140 FSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIVS 199

Query: 188 SIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDACSETNVL 247
           S+FSLL++VTWL+KDI+WLVADSRYG+Y  V+AWL +YH+P F  S ++  D        
Sbjct: 200 SLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCD-------- 259

Query: 248 DEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLIN 307
                    E+   D+F+RAGTMAAALV+KV  RSE FED+LS+YAEASNGQMPNLDLIN
Sbjct: 260 ---------EQNTADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLIN 319

Query: 308 IVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGMSASDYVD 367
           +VNYLAVHRQGF +K+EK   LL+  WLK+ GE+FE++GK+   LN +W FG+ A+DY++
Sbjct: 320 VVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLE 379

Query: 368 GTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFILRGGRLIE 427
           G+ATLASSLY QA+GIPTG HGAFRDYQ+DAIT+++SP+F    K R+ DF LRG RL+E
Sbjct: 380 GSATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLLE 439

Query: 428 GVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALYSYANNLN 487
           G +RSVNNLLEKFHQSFFLY++ S  KF+SVGVYMIAFALLVAPLP VAA+LY       
Sbjct: 440 GTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLY------- 499

Query: 488 LTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQIPG-YSP 547
           +   K    +  NP +    + +SWKWL+AAK+VF +HL G IV+LLPYFICQ+PG +SP
Sbjct: 500 IDGCKSLTNSTHNPTE----NFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSP 559

Query: 548 TENSIIWGLLSLLSLLVLSVILG--SPFSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMS 607
           T  SI+WG  S   LL+  V +   SPFSS          WA LK++T SAAFIGLCLMS
Sbjct: 560 TNRSIMWGTTSSSLLLITFVTMPGCSPFSS----RLHGTNWAILKSVTISAAFIGLCLMS 619

Query: 608 VINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFFLFKG 667
           +INF+TAE+GA L+V  CL+A P+K  L +   K+L  A C++VL  I FP + F + KG
Sbjct: 620 IINFATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKG 679

Query: 668 AL-QGFDNLHI-GDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHP 714
            L +G   L + G+FW W+E+LWAW SAT+LY+GMVHLPCWLLC  IL HP
Sbjct: 680 LLGEGLVGLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 698

BLAST of Csa5G523060 vs. NCBI nr
Match: gi|778703612|ref|XP_004142789.2| (PREDICTED: glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Cucumis sativus])

HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 714/714 (100.00%), Postives = 714/714 (100.00%), Query Frame = 1

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
           SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS
Sbjct: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT
Sbjct: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240

Query: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360
           PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360

Query: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420
           SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540
           SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ
Sbjct: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540

Query: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660
           CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC 715
           LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC
Sbjct: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC 714

BLAST of Csa5G523060 vs. NCBI nr
Match: gi|659117821|ref|XP_008458804.1| (PREDICTED: glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Cucumis melo])

HSP 1 Score: 1376.3 bits (3561), Expect = 0.0e+00
Identity = 686/714 (96.08%), Postives = 701/714 (98.18%), Query Frame = 1

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKLK KARPIVRLGIFLISHSIFFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
           SAN+MLSGREVSEANNLVKDLKGLNSKPGSSIF SQQILA+YISKLGADVNYHRFH QSS
Sbjct: 61  SANTMLSGREVSEANNLVKDLKGLNSKPGSSIFESQQILARYISKLGADVNYHRFHAQSS 120

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           QFHPLHFFSSPDSGIL+DNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT
Sbjct: 121 QFHPLHFFSSPDSGILQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQS VIDTDA
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSGVIDTDA 240

Query: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           C+ETNVL+ FEAN VTEK ILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CTETNVLNGFEANHVTEKWILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360
           PNLDLINIVNYLAVHRQGFRIKIEKFWPLL+CKWLKVLGEVFESIGK+IRSLNSEWKFGM
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGKIIRSLNSEWKFGM 360

Query: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420
           SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSG+KVRRDDFIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480
           RGGRL+EGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY
Sbjct: 421 RGGRLVEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540
           S+ANNLNLTSEKVEPPA  NPDDELIVSLRSWKWLNAAKRVFVVHLWGA+VSLLPYFICQ
Sbjct: 481 SHANNLNLTSEKVEPPAPENPDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTENSIIWGLLSLL+LLVLSVILG P SSTKSYEQ IQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLALLVLSVILGCPLSSTKSYEQGIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660
           CLMSVINF+TAE+GAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAEIGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC 715
           LFK ALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC 714

BLAST of Csa5G523060 vs. NCBI nr
Match: gi|778703616|ref|XP_011655396.1| (PREDICTED: glycosylphosphatidylinositol anchor attachment 1 protein isoform X2 [Cucumis sativus])

HSP 1 Score: 1342.0 bits (3472), Expect = 0.0e+00
Identity = 670/670 (100.00%), Postives = 670/670 (100.00%), Query Frame = 1

Query: 45  MFAMNTYISENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYIS 104
           MFAMNTYISENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYIS
Sbjct: 1   MFAMNTYISENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYIS 60

Query: 105 KLGADVNYHRFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAI 164
           KLGADVNYHRFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAI
Sbjct: 61  KLGADVNYHRFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAI 120

Query: 165 VLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRD 224
           VLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRD
Sbjct: 121 VLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRD 180

Query: 225 YHTPVFGQSSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEH 284
           YHTPVFGQSSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEH
Sbjct: 181 YHTPVFGQSSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEH 240

Query: 285 FEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFES 344
           FEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFES
Sbjct: 241 FEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFES 300

Query: 345 IGKVIRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS 404
           IGKVIRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS
Sbjct: 301 IGKVIRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS 360

Query: 405 PKFSSGIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIA 464
           PKFSSGIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIA
Sbjct: 361 PKFSSGIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIA 420

Query: 465 FALLVAPLPAVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVV 524
           FALLVAPLPAVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVV
Sbjct: 421 FALLVAPLPAVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVV 480

Query: 525 HLWGAIVSLLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQE 584
           HLWGAIVSLLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQE
Sbjct: 481 HLWGAIVSLLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQE 540

Query: 585 WAFLKAMTTSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAA 644
           WAFLKAMTTSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAA
Sbjct: 541 WAFLKAMTTSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAA 600

Query: 645 CNLVLGFIAFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWL 704
           CNLVLGFIAFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWL
Sbjct: 601 CNLVLGFIAFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWL 660

Query: 705 LCTQILLHPC 715
           LCTQILLHPC
Sbjct: 661 LCTQILLHPC 670

BLAST of Csa5G523060 vs. NCBI nr
Match: gi|659117823|ref|XP_008458805.1| (PREDICTED: glycosylphosphatidylinositol anchor attachment 1 protein isoform X2 [Cucumis melo])

HSP 1 Score: 1297.0 bits (3355), Expect = 0.0e+00
Identity = 644/670 (96.12%), Postives = 658/670 (98.21%), Query Frame = 1

Query: 45  MFAMNTYISENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYIS 104
           MFAMNTYISENALMPGSAN+MLSGREVSEANNLVKDLKGLNSKPGSSIF SQQILA+YIS
Sbjct: 1   MFAMNTYISENALMPGSANTMLSGREVSEANNLVKDLKGLNSKPGSSIFESQQILARYIS 60

Query: 105 KLGADVNYHRFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAI 164
           KLGADVNYHRFH QSSQFHPLHFFSSPDSGIL+DNVSCMTQGINTVGIIRAPQADGKEAI
Sbjct: 61  KLGADVNYHRFHAQSSQFHPLHFFSSPDSGILQDNVSCMTQGINTVGIIRAPQADGKEAI 120

Query: 165 VLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRD 224
           VLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRD
Sbjct: 121 VLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRD 180

Query: 225 YHTPVFGQSSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEH 284
           YHTPVFGQS VIDTDAC+ETNVL+ FEAN VTEK ILDDFKRAGTMAAALVIKVSNRSEH
Sbjct: 181 YHTPVFGQSGVIDTDACTETNVLNGFEANHVTEKWILDDFKRAGTMAAALVIKVSNRSEH 240

Query: 285 FEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFES 344
           FEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLL+CKWLKVLGEVFES
Sbjct: 241 FEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFES 300

Query: 345 IGKVIRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS 404
           IGK+IRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS
Sbjct: 301 IGKIIRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS 360

Query: 405 PKFSSGIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIA 464
           PKFSSG+KVRRDDFILRGGRL+EGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIA
Sbjct: 361 PKFSSGVKVRRDDFILRGGRLVEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIA 420

Query: 465 FALLVAPLPAVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVV 524
           FALLVAPLPAVAAALYS+ANNLNLTSEKVEPPA  NPDDELIVSLRSWKWLNAAKRVFVV
Sbjct: 421 FALLVAPLPAVAAALYSHANNLNLTSEKVEPPAPENPDDELIVSLRSWKWLNAAKRVFVV 480

Query: 525 HLWGAIVSLLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQE 584
           HLWGA+VSLLPYFICQIPGYSPTENSIIWGLLSLL+LLVLSVILG P SSTKSYEQ IQE
Sbjct: 481 HLWGAVVSLLPYFICQIPGYSPTENSIIWGLLSLLALLVLSVILGCPLSSTKSYEQGIQE 540

Query: 585 WAFLKAMTTSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAA 644
           WAFLKAMTTSAAFIGLCLMSVINF+TAE+GAFLVVSMCLLAHPLKLDLGAGNFKALSRAA
Sbjct: 541 WAFLKAMTTSAAFIGLCLMSVINFATAEIGAFLVVSMCLLAHPLKLDLGAGNFKALSRAA 600

Query: 645 CNLVLGFIAFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWL 704
           CNLVLGFIAFPPVTFFLFK ALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWL
Sbjct: 601 CNLVLGFIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWL 660

Query: 705 LCTQILLHPC 715
           LCTQILLHPC
Sbjct: 661 LCTQILLHPC 670

BLAST of Csa5G523060 vs. NCBI nr
Match: gi|645248980|ref|XP_008230539.1| (PREDICTED: glycosylphosphatidylinositol anchor attachment 1 protein [Prunus mume])

HSP 1 Score: 984.2 bits (2543), Expect = 1.2e-283
Identity = 475/715 (66.43%), Postives = 583/715 (81.54%), Query Frame = 1

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           +AE E  +PK RPIV+LGIFLISHS+ FSV+  +AGVLALLLLP+ A NTYISENALMPG
Sbjct: 2   VAEKETPRPKPRPIVQLGIFLISHSLLFSVIFSTAGVLALLLLPVLAKNTYISENALMPG 61

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
           S   MLS +EV+EAN LVKDL  LNSKP SS  GSQ++++QY+S LGA+V++H+FHPQ +
Sbjct: 62  SVAPMLSNQEVAEANRLVKDLTSLNSKPLSSAIGSQRLISQYMSNLGAEVSHHKFHPQLN 121

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           QFHPLHFFSSPDSG ++ N+SC   G+NTVGIIRAP+ DGKEAIVLVTP+N V+ + ++ 
Sbjct: 122 QFHPLHFFSSPDSGRVEQNISCALHGVNTVGIIRAPRGDGKEAIVLVTPFNSVKLNQNEA 181

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240
           LSL IAYS+FSLLT+V WLAKD++WLVADS++GEY+ V+AWLRDYHTPVF     +D + 
Sbjct: 182 LSLAIAYSVFSLLTRVPWLAKDVIWLVADSQFGEYSGVAAWLRDYHTPVFSGPGTLDAEM 241

Query: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           C E+N +     N  TE+ I D FKRAGTMAAALVIKV++RSE +EDSLS+YAEASNGQM
Sbjct: 242 CPESNNIYGLNENLFTERTIYDGFKRAGTMAAALVIKVADRSEQYEDSLSIYAEASNGQM 301

Query: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360
           PNLDLINIVNYLAVHRQG R+K+EKFWPLL  K LK LGE+FES+G + RS+N +WKFG+
Sbjct: 302 PNLDLINIVNYLAVHRQGLRVKVEKFWPLLGLKLLKTLGEIFESLGHLARSVNPQWKFGI 361

Query: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSS-GIKVRRDDFI 420
            A+DY++G+ATLASSLYYQA+G+PTG HGAFRDYQ+DAIT+E+ PK SS   KVR++DF+
Sbjct: 362 PAADYIEGSATLASSLYYQALGVPTGPHGAFRDYQVDAITLEIFPKVSSLDNKVRQNDFL 421

Query: 421 LRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAAL 480
           LR GRLIEGV+RSVNNLLEKFHQSFFLYL+ S GKFVSVGVYMIAFALLVAPLP VAA+L
Sbjct: 422 LRSGRLIEGVIRSVNNLLEKFHQSFFLYLLTSPGKFVSVGVYMIAFALLVAPLPMVAASL 481

Query: 481 YSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFIC 540
           Y  A+N + + +  +P  LAN   E  ++L+SWKWL AAK+VF VHLWG+ VSLLPYFIC
Sbjct: 482 YVDASNSDYSLKIDKPAPLANASGEPCITLKSWKWLFAAKKVFFVHLWGSAVSLLPYFIC 541

Query: 541 QIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIG 600
           QIP  +PT + IIW  LS+LSL+V+ ++L SP S     + +  EWA LK++T SAAFIG
Sbjct: 542 QIPSCTPTSSFIIWVFLSILSLVVMYIVLASPSSHANESQPQRGEWAILKSVTLSAAFIG 601

Query: 601 LCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTF 660
           LCLMSVINF+TAE+G  L+V MCL+AHPLKLD+ A + + +SR   NLVLGFI FPPV F
Sbjct: 602 LCLMSVINFATAEIGGLLMVPMCLMAHPLKLDVKARSIRTVSRVVYNLVLGFIGFPPVAF 661

Query: 661 FLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC 715
            + KGA +GF  +++GDFW+W E+LWAWNS T+LY+GMVHLPCW+LC  IL H C
Sbjct: 662 IVLKGAFEGFSGVNVGDFWSWAESLWAWNSGTYLYIGMVHLPCWVLCIHILFHRC 716