Csa5G318390 (gene) Cucumber (Chinese Long) v2

NameCsa5G318390
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
Description2-succinylbenzoate-CoA ligase; contains IPR000873 (AMP-dependent synthetase/ligase), IPR025110 (Domain of unknown function DUF4009)
LocationChr5 : 12917490 .. 12920263 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGTATAGTTGTAGCATTCTCCTCCCTCCTCATCCTTGTTCGCGGCCCGTTTCTTCATCGGTAATTTTTACATCTTTTCTTGACATTAAATATACTTTTATACCTTGTTCATTTAAAACTTAAAAGTGATTGTACAAAATATGGTTGTCCACAAAAACCACACTCAACTTAATACAAACATTGTCTTACATGAATATTCCTTAAAAGAATTATAAGAATGTAAAAAGTTTTAAATAAGTATACGAAATTAATTTTTTGTTTATTTTTTATTTTTGCAATTTTTTACCATATAATAAAATGTGTCAATTCACTCCTTACGTAAATGATGTTAAATTTGTAGAAGTAGCTAAAAATGTCAAATGTGTTATATGCTTCTTCATTCTGTTTTTTCTTTTTCCTTCCTCATTTCTCTTTGTTTATAATATTGCTGCTTTTTCCATTTCTTTCTACTTCTTTCTTCTTCTTCTATTTCTATACTCTTTTTTTTTTTTTTTCATTTCAAGGGAGCAACATTTTTTCCCCTAAATTTTGTTTTTCATAACGCAGTCCATCAACTTTTCTCTTTATTTAATTTTAATATTTTTAAGTTTACAATTTTCAGCAAAGCAAAACGAATGTTAGAATAACAATAATGGATATCTTATTTATAAAAAATTATTTGAGATTTAAGGTTCCTGCAATGTCGACTAGCTATGTCAATAGATGGAGTTTGAATGAACACTTGTTAAAATGCTTTAATTAAAGAGAAATTGTTGAAAGCATGTTGGATTAACATCGTCTAAGATGAGTTAGTTAACTTGTTAGAACCTTCATTATTTATACTTGCATGCAGAGGAAGAAAATAATAAAGCAGCAACAAAAAAGAAGAGAGCATAGAATATACTTTCTATGGAAGATCTGAAGCCAAGGCCTGCTAATTCCTTTCCCCTCACTCCAATCGGCTTCATCGAAAGAGCCGCCATCATTTATGGGGATTCTACCTCCATTATCTACAACTCCACCACTTACACCTGGTCCGAAACTCATCGTCGATGCCTTCAACTCGCCTCTTCCATCTCTTCCTTTGGCATTCAAAGAGGCCATGTTGTCTCTGTCTTCGCTCCCAACACTCCCGCCATGTACGAACTCCACTTCGCTGTTCCCATGGCTGGTGCCATTCTTAATACCATCAATCTCCGCCTTGACACTCGAACCATCTCCATTCTCCTCCGCCATAGCGAATCCAAACTCATCTTCGTCGATCAACCCTCTTGTGCTCTAATCTTTGACGCTTTGGCCTTGTTCCCGCCAGACATGAAACGACCCTTGCTTGTCCTCATTACCGACGACACTGCAGAGCAAGACTCGTCATTGCCATCCGTTGTTTCCATCCGAGACAATTTTGTGGATTCATATGAGAACATGGTTAAGAGTGGAGATTCTAAGTTCCAATGGATACGACCCTTAAGTGAGTGGGATCCGATTGTTTTGAATTATACTTCTGGAACAACATCAGCGCCAAAGGGAGTGGTAAACTGTCACAGGGGAGTTTTCACGGTGGCACTCGATTCAATTTTGGAATGGGGAGTTCCTAAGCAATCGGTTTATTTATGGACCTTACCAATGTTTCATGGCAATGGTTGGAGTTTCCCTTGGGGAATTGCCGCTGTAGGTGGCACAAATGTTTGTATTCGAAAATTTGATTCAAGATTGATTTTTTCTCTAATTCGTCGTCATCACGTGACACACTTGTGTGGAGCTCCAGTTGTACTCAATATGCTAACAAACTCCCCTGACAATCTACCACTTGATCGACCGGTCAAGATTCTCACTGCCGGAGCACCACCACCCGCATCAGTTCTGCTTCGAACTGAATCACTGGGGTTTGTCGTCACCCATGGGTACGGATTGACTGAGAGTGCAGGGACGATCATCTGCTGTTCATGGAAGTCGGAGTGGAACCAACTCCCAGCAATAGAGCAAGCACGACTGAAGGCGAGGCAGGGCGTACGGATGCTAGCAGTGGAGGCGGTGGATGTGTTGGATCCAGAGACTAGAAAAAGCGTGAAAAGAGATGGAGCTTCCATAGGAGAGATCGTTGTGAGAGGTCCATCGCTAATGCTTGGGTATTTGAAAGATACAGAAGCAACAAGTAAAGCAATGAGCGAGGATGGGTGGTTCTTCACCGGAGATGTTGGGGTGATGCACCCGGATGGGTATATAGAGATAAAGGACCGATCAAAGGATGTGATCATTAGTGGGGGAGAGAATTTGAGCAGTGTTGAGGTTGAATCAGTTTTGTACATGAATCCAATGGTGAATGAGGCGGCAGTGGTGGCACGACCAGACGAGTTCTGGGGAGAGACGCCATGTGCGTTTATAAGCTTGAAAGAAGGATTGAGTCGGAAACCAACAGAAGAAGAGATTATTGAGTATTGCAAAGGGAAGTTACCAAAGTTTATGGTGCCAAAGACAGTGATCTTCATGACGGAAATGCCAAAGACCGCCACAGGGAAATTCCAAAAGTTTGTGCTTAGACAAAAGGCCAAGTCACTAGGCCCTCCACAAAAGAGTAGATTGTAGTAAAAAATCATAAGTATCTACATCTAAATCTATCTCCAGTATCGATAATAAATATTAGGGTGACGATTTAATCGTTTCCAATCGGATGGATTAGTTTTCTTTTGTTATTTCATGATAATTTGGTTACAATGTGGAGTCGGGTTCGTATGATTTTAGAAATAAATATCTCATGTTTTATGATATGAAGGCAATTATGAAGTTTTATATATATT

mRNA sequence

ATGGAAGATCTGAAGCCAAGGCCTGCTAATTCCTTTCCCCTCACTCCAATCGGCTTCATCGAAAGAGCCGCCATCATTTATGGGGATTCTACCTCCATTATCTACAACTCCACCACTTACACCTGGTCCGAAACTCATCGTCGATGCCTTCAACTCGCCTCTTCCATCTCTTCCTTTGGCATTCAAAGAGGCCATGTTGTCTCTGTCTTCGCTCCCAACACTCCCGCCATGTACGAACTCCACTTCGCTGTTCCCATGGCTGGTGCCATTCTTAATACCATCAATCTCCGCCTTGACACTCGAACCATCTCCATTCTCCTCCGCCATAGCGAATCCAAACTCATCTTCGTCGATCAACCCTCTTGTGCTCTAATCTTTGACGCTTTGGCCTTGTTCCCGCCAGACATGAAACGACCCTTGCTTGTCCTCATTACCGACGACACTGCAGAGCAAGACTCGTCATTGCCATCCGTTGTTTCCATCCGAGACAATTTTGTGGATTCATATGAGAACATGGTTAAGAGTGGAGATTCTAAGTTCCAATGGATACGACCCTTAAGTGAGTGGGATCCGATTGTTTTGAATTATACTTCTGGAACAACATCAGCGCCAAAGGGAGTGGTAAACTGTCACAGGGGAGTTTTCACGGTGGCACTCGATTCAATTTTGGAATGGGGAGTTCCTAAGCAATCGGTTTATTTATGGACCTTACCAATGTTTCATGGCAATGGTTGGAGTTTCCCTTGGGGAATTGCCGCTGTAGGTGGCACAAATGTTTGTATTCGAAAATTTGATTCAAGATTGATTTTTTCTCTAATTCGTCGTCATCACGTGACACACTTGTGTGGAGCTCCAGTTGTACTCAATATGCTAACAAACTCCCCTGACAATCTACCACTTGATCGACCGGTCAAGATTCTCACTGCCGGAGCACCACCACCCGCATCAGTTCTGCTTCGAACTGAATCACTGGGGTTTGTCGTCACCCATGGGTACGGATTGACTGAGAGTGCAGGGACGATCATCTGCTGTTCATGGAAGTCGGAGTGGAACCAACTCCCAGCAATAGAGCAAGCACGACTGAAGGCGAGGCAGGGCGTACGGATGCTAGCAGTGGAGGCGGTGGATGTGTTGGATCCAGAGACTAGAAAAAGCGTGAAAAGAGATGGAGCTTCCATAGGAGAGATCGTTGTGAGAGGTCCATCGCTAATGCTTGGGTATTTGAAAGATACAGAAGCAACAAGTAAAGCAATGAGCGAGGATGGGTGGTTCTTCACCGGAGATGTTGGGGTGATGCACCCGGATGGGTATATAGAGATAAAGGACCGATCAAAGGATGTGATCATTAGTGGGGGAGAGAATTTGAGCAGTGTTGAGGTTGAATCAGTTTTGTACATGAATCCAATGGTGAATGAGGCGGCAGTGGTGGCACGACCAGACGAGTTCTGGGGAGAGACGCCATGTGCGTTTATAAGCTTGAAAGAAGGATTGAGTCGGAAACCAACAGAAGAAGAGATTATTGAGTATTGCAAAGGGAAGTTACCAAAGTTTATGGTGCCAAAGACAGTGATCTTCATGACGGAAATGCCAAAGACCGCCACAGGGAAATTCCAAAAGTTTGTGCTTAGACAAAAGGCCAAGTCACTAGGCCCTCCACAAAAGAGTAGATTGTAG

Coding sequence (CDS)

ATGGAAGATCTGAAGCCAAGGCCTGCTAATTCCTTTCCCCTCACTCCAATCGGCTTCATCGAAAGAGCCGCCATCATTTATGGGGATTCTACCTCCATTATCTACAACTCCACCACTTACACCTGGTCCGAAACTCATCGTCGATGCCTTCAACTCGCCTCTTCCATCTCTTCCTTTGGCATTCAAAGAGGCCATGTTGTCTCTGTCTTCGCTCCCAACACTCCCGCCATGTACGAACTCCACTTCGCTGTTCCCATGGCTGGTGCCATTCTTAATACCATCAATCTCCGCCTTGACACTCGAACCATCTCCATTCTCCTCCGCCATAGCGAATCCAAACTCATCTTCGTCGATCAACCCTCTTGTGCTCTAATCTTTGACGCTTTGGCCTTGTTCCCGCCAGACATGAAACGACCCTTGCTTGTCCTCATTACCGACGACACTGCAGAGCAAGACTCGTCATTGCCATCCGTTGTTTCCATCCGAGACAATTTTGTGGATTCATATGAGAACATGGTTAAGAGTGGAGATTCTAAGTTCCAATGGATACGACCCTTAAGTGAGTGGGATCCGATTGTTTTGAATTATACTTCTGGAACAACATCAGCGCCAAAGGGAGTGGTAAACTGTCACAGGGGAGTTTTCACGGTGGCACTCGATTCAATTTTGGAATGGGGAGTTCCTAAGCAATCGGTTTATTTATGGACCTTACCAATGTTTCATGGCAATGGTTGGAGTTTCCCTTGGGGAATTGCCGCTGTAGGTGGCACAAATGTTTGTATTCGAAAATTTGATTCAAGATTGATTTTTTCTCTAATTCGTCGTCATCACGTGACACACTTGTGTGGAGCTCCAGTTGTACTCAATATGCTAACAAACTCCCCTGACAATCTACCACTTGATCGACCGGTCAAGATTCTCACTGCCGGAGCACCACCACCCGCATCAGTTCTGCTTCGAACTGAATCACTGGGGTTTGTCGTCACCCATGGGTACGGATTGACTGAGAGTGCAGGGACGATCATCTGCTGTTCATGGAAGTCGGAGTGGAACCAACTCCCAGCAATAGAGCAAGCACGACTGAAGGCGAGGCAGGGCGTACGGATGCTAGCAGTGGAGGCGGTGGATGTGTTGGATCCAGAGACTAGAAAAAGCGTGAAAAGAGATGGAGCTTCCATAGGAGAGATCGTTGTGAGAGGTCCATCGCTAATGCTTGGGTATTTGAAAGATACAGAAGCAACAAGTAAAGCAATGAGCGAGGATGGGTGGTTCTTCACCGGAGATGTTGGGGTGATGCACCCGGATGGGTATATAGAGATAAAGGACCGATCAAAGGATGTGATCATTAGTGGGGGAGAGAATTTGAGCAGTGTTGAGGTTGAATCAGTTTTGTACATGAATCCAATGGTGAATGAGGCGGCAGTGGTGGCACGACCAGACGAGTTCTGGGGAGAGACGCCATGTGCGTTTATAAGCTTGAAAGAAGGATTGAGTCGGAAACCAACAGAAGAAGAGATTATTGAGTATTGCAAAGGGAAGTTACCAAAGTTTATGGTGCCAAAGACAGTGATCTTCATGACGGAAATGCCAAAGACCGCCACAGGGAAATTCCAAAAGTTTGTGCTTAGACAAAAGGCCAAGTCACTAGGCCCTCCACAAAAGAGTAGATTGTAG

Protein sequence

MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKFVLRQKAKSLGPPQKSRL*
BLAST of Csa5G318390 vs. Swiss-Prot
Match: AAE5_ARATH (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana GN=AAE5 PE=2 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 7.2e-215
Identity = 359/558 (64.34%), Postives = 452/558 (81.00%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTT-YTWSETHRRCLQLASSISSF 60
           ME +KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV I D   +++    + V+ R  F  +Y++++  GD  
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIAD---KEEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGWS+PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + +  P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+RPV ILTAGAPPPA+VLLR ES+GFV++HGYGLTE+AG  + C+WK +WN+LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+ +SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           D FWGETPCAF+SLK GL+++PTE E+IEYC+ K+PK+MVPKTV F+ E+PKT+TGK  K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQKAKSLGPPQKSRL 558
           FVLR+ AK +G  + SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of Csa5G318390 vs. Swiss-Prot
Match: AAE6_ARATH (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana GN=AAE6 PE=2 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 8.0e-214
Identity = 357/551 (64.79%), Postives = 447/551 (81.13%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTT-YTWSETHRRCLQLASSISSF 60
           ME++KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV+I D   E++  +  V  +   F  +Y+++++ GD  
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIADK--EEEGGVADVADL-SKFSYTYDDLIERGDPG 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGW+ PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + ++ P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+ PV ILTAG+PPPA+VLLR ES+GFV++HGYGLTE+AG I+ C+WK +WN LPA ++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+  SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           DEFWGETPCAF+SLK G S KPTEEE++EYC+ K+PK+MVPKTV FM E+PK++TGK  K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQKAKSLG 551
           FVLR  AK +G
Sbjct: 541 FVLRDIAKKMG 545

BLAST of Csa5G318390 vs. Swiss-Prot
Match: AAE8_ARATH (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana GN=AAE8 PE=2 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 4.1e-202
Identity = 342/551 (62.07%), Postives = 433/551 (78.58%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSISSF 60
           MEDLKP  ANS PLT +GF+ERAA +YGD TSI+Y NST YTW ET+ RCL +AS++SS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV + NTP MYEL F+VPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
               L  +A+ +    +  P+LVLI ++  E+  +    V+ R  F   Y +++  G+  
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGGA---EVTERSKFCYLYSDLITRGNPD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDPIV+NYTSGTTS+PKGVV+CHRG+F + LDS+ +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN-L 300
           FH NGW++PWGIAAVGGTNVC+RK  +  I+ LIR H VTH+ GAP+VL +L+ S ++  
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV  LTAG+ PPA+VLLR ESLGF+V+HGYGLTE+AG I+ C+WK  WN+LPA +Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAM 420
           A+LK+RQGVR +    +DV+DPE+ +SV+RDG ++GEIV+RG S+MLGYLK+   T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 SEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVAR 480
            ++GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNEAAVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQ 540
           PDEFWGETPCAF+SLK GL+RKPT++EIIEYCK K+P++M PKTV F+ E+PKT+TGK  
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLRQKAKSL 550
           K +L++ AK++
Sbjct: 541 KSLLKEIAKNM 544

BLAST of Csa5G318390 vs. Swiss-Prot
Match: AEE9_ARATH (Probable acyl-activating enzyme 9 OS=Arabidopsis thaliana GN=AEE9 PE=2 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 3.4e-196
Identity = 330/555 (59.46%), Postives = 426/555 (76.76%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTT-YTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++ + T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI RG VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSL-PSVVSIRDNFVDSYENMVKSG 180
           D  S +++ +A++ F    ++P LVL+ DD  ++D SL PS  S   +F+D+Y+ +++ G
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAAS---DFLDTYQGVMERG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWT 240
           DS+F+WIRP +EW P++LNYTSGTTS+PKGVV  HR +F + + S+L+W  P + VYLWT
Sbjct: 181 DSRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGW + WG AAVG TNVC R+ D+  I+ LI +HHVTH+C AP+VLNMLTN P 
Sbjct: 241 LPMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPS 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
             PL  PV+++TAGAPPPA+++ R E+LGF V HGYGLTE+ G ++ C+WK+EW+ L  +
Sbjct: 301 RKPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPL 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLK+RQGVR +    VDV DP T KSV+ DG S+GEIV++G S+MLGY KD E T+ 
Sbjct: 361 ERARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAA 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
            M EDGWF++GDVGV+H DGY+E+KDRSKDVII GGEN+SS EVE+VLY NP+V EAAVV
Sbjct: 421 CMREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRK--PTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTAT 540
           A+PD+ WGETPCAF+SLK   +     TE EI E+CK +LPK+MVP+ VIF  E+PKT+T
Sbjct: 481 AKPDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKFQKFVLRQKAKSL 550
           GK QKF+LRQ AKSL
Sbjct: 541 GKIQKFLLRQMAKSL 549

BLAST of Csa5G318390 vs. Swiss-Prot
Match: AEE10_ARATH (Probable acyl-activating enzyme 10 OS=Arabidopsis thaliana GN=AEE10 PE=2 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 5.4e-194
Identity = 329/556 (59.17%), Postives = 426/556 (76.62%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTT-YTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++ + T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI++G VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSES+L+FV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGD 180
           D  S +L+ +A++LF    K P LVL+ DD  E DSS  S      +F+D+YE +++ G+
Sbjct: 121 DHRSISLVLEAVSLFTQHEK-PHLVLLDDDQ-ENDSSSAS------DFLDTYEEIMERGN 180

Query: 181 SKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTL 240
           S+F+WIRP +EW P+VLNYTSGTTS+PKGVV  HR +F + + S+L+W VP + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTL 240

Query: 241 PMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN 300
           PMFH NGW + WG AAVG TN+C R+ D+  I++LI +H+VTH+C AP+VLNML N P +
Sbjct: 241 PMFHANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLS 300

Query: 301 LPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIE 360
            PL  PV+++T+GAPPPA+++ R ESLGF V+H YGLTE++G ++ C+WK +W+ L  +E
Sbjct: 301 TPLKNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLE 360

Query: 361 QARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKA 420
           +ARLK+RQGVR L    VDV D +T KSVK DG S+GEIV RG S+MLGY KD + T+  
Sbjct: 361 RARLKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAAC 420

Query: 421 MSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVA 480
           M EDGWF++GD+GV+H DGY+EIKDRSKDVII GGEN+SS E+E+VLY NP+V EAAVVA
Sbjct: 421 MREDGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVA 480

Query: 481 RPDEFWGETPCAFISLK---EGLSRKP-TEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTA 540
           +PD+ WGETPCAF+SLK    G    P TE EI E+CK KLPK+MVP+ VIF  E+PKT+
Sbjct: 481 KPDKMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTS 540

Query: 541 TGKFQKFVLRQKAKSL 550
           TGK QKF+LRQ AK+L
Sbjct: 541 TGKIQKFLLRQMAKTL 548

BLAST of Csa5G318390 vs. TrEMBL
Match: A0A0A0KT33_CUCSA (AMP dependent ligase OS=Cucumis sativus GN=Csa_5G317890 PE=4 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 6.5e-255
Identity = 432/557 (77.56%), Postives = 490/557 (87.97%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           ME LKP PANS PLTPIGF+ERA++IY DS SIIY+STT+TWS+T+RRCL+LASS+SS G
Sbjct: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I +GHVVSV APNTP MYEL FAVPMAGAIL+ INLRLD RTIS+ LRHSESKL+FVDQ 
Sbjct: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S  LI DALALFPP+ KRPLLVLI+DD A +DS+  S V+I  + VD YE+MV+ GD +F
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSA--SSVAIGGDVVDLYEDMVQKGDPEF 201

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDPIV+NYTSGTTS+PKGVV+ HRG+FTV LDS++EWGVPK SVYLWTLPMF
Sbjct: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFP+ IAAVGGTN+CIRKFD+ LIFSLI RHHVTH+CGAPVVLNMLTNSP+N PL
Sbjct: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           +RPVKILTAGAPPPA+VLLRTESLGF V+HGYGLTE+ G +I CSWK EWNQLPA E+AR
Sbjct: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR LA+   DVLD  T K V+RDG SIGEIV+RG S+MLGY KD EAT KAM+E
Sbjct: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SL+EGL+R PTEEEIIEYCKGKL  FMVPKTV+FM ++PKT+TGK QKF
Sbjct: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561

Query: 541 VLRQKAKSLGPPQKSRL 558
           +LR+ AKS+G   KSRL
Sbjct: 562 LLREMAKSMGCSPKSRL 576

BLAST of Csa5G318390 vs. TrEMBL
Match: A0A061ESR0_THECC (Acyl activating enzyme 5 OS=Theobroma cacao GN=TCM_020359 PE=4 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 2.2e-234
Identity = 383/550 (69.64%), Postives = 469/550 (85.27%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           ME+LKPRPA+S PLTP+GF+ERAA +YGD TSIIYN+T+YTWS+THRRCLQ+ASS+SS G
Sbjct: 51  MEELKPRPASSSPLTPLGFLERAATVYGDCTSIIYNNTSYTWSDTHRRCLQIASSLSSTG 110

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I+RGHVVSV APN PAMYELHFAVPM GA+LN+IN RLD R +SILLRH ESKL+FVD  
Sbjct: 111 IKRGHVVSVLAPNIPAMYELHFAVPMTGAVLNSINTRLDARIVSILLRHCESKLLFVDTL 170

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S +L  +A++LFPP+ K PLLVLI DD+A+ +SS  S  ++   F  +YE++VK GD  F
Sbjct: 171 SRSLALEAISLFPPNTKPPLLVLIGDDSADAESSDSSSSTVDSRFCCTYESLVKKGDPDF 230

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +W+RP SEW+PIVLNYTSGTTS+PKGVV+ HRG F + LDS+++WGVPKQ VYLWTLPMF
Sbjct: 231 KWVRPQSEWNPIVLNYTSGTTSSPKGVVHSHRGTFMMTLDSLIDWGVPKQPVYLWTLPMF 290

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSF WGIAAVGG NVC+RKFD+ +I+SLI++H VTH+CGAPVVL+ML+N P+  PL
Sbjct: 291 HANGWSFTWGIAAVGGINVCVRKFDAPIIYSLIKKHGVTHMCGAPVVLSMLSNFPEIKPL 350

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
             PVKILTAGAPPPA+VL RTE+LGF+V+HGYGLTE+ G ++ C+WK  WN+LPA E+AR
Sbjct: 351 QSPVKILTAGAPPPAAVLFRTEALGFIVSHGYGLTETGGLVVSCAWKRGWNKLPATERAR 410

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR L +   DV+D E+  SVKRDG+++GEIV+RG  +MLGYLKD   T+K M E
Sbjct: 411 LKARQGVRTLGMTEADVVDTESGLSVKRDGSTLGEIVLRGACIMLGYLKDPNGTNKCMKE 470

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LY +P + EAAVVARPD
Sbjct: 471 NGWFFTGDVGVMHPDGYMEIKDRSKDVIISGGENLSSVEVESILYNHPAIIEAAVVARPD 530

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           ++WGETPCAF+SLK GL++KP+E+EIIEYC+ KLP +MVPKTV+F  E+PKT+TGK QKF
Sbjct: 531 DYWGETPCAFVSLKAGLTQKPSEKEIIEYCRAKLPHYMVPKTVVFKEELPKTSTGKIQKF 590

Query: 541 VLRQKAKSLG 551
           +LR+ AK +G
Sbjct: 591 LLREIAKGMG 600

BLAST of Csa5G318390 vs. TrEMBL
Match: A0A0D2VMX2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G160500 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 8.2e-234
Identity = 386/550 (70.18%), Postives = 467/550 (84.91%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           ME+LK RPANS PLTP+GF++RAA +YGD TSII+N+T+YTWSETHRRCLQLASS+SS G
Sbjct: 1   MEELKSRPANSSPLTPLGFLDRAATVYGDCTSIIHNNTSYTWSETHRRCLQLASSLSSTG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I+ GHVVSV APN PAMYELHFAVPM+GA+LN+IN RLD RT+SILL HSESKL+FVD  
Sbjct: 61  IKTGHVVSVLAPNIPAMYELHFAVPMSGAVLNSINTRLDARTVSILLCHSESKLLFVDTL 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S +L  +A++LFPP+   PLLVLI DD   +D  + S +  R  F  +YE++V+ G+  F
Sbjct: 121 SQSLALEAISLFPPNENPPLLVLIKDD---EDDGVSSTIDSR--FCCTYESLVEKGNPGF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +WIRP SEW+PIVLNYTSGTTS+PKGVV+CHR VFT  +DS++ W VPKQ VYLWTLPMF
Sbjct: 181 KWIRPESEWNPIVLNYTSGTTSSPKGVVHCHRAVFTNTVDSLIHWEVPKQPVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSF WG+AAVGGTN+C+RKFD+R+I+SLIR+H VTH+CGAPVVLNML+NSP+  PL
Sbjct: 241 HANGWSFTWGMAAVGGTNICVRKFDARIIYSLIRKHGVTHMCGAPVVLNMLSNSPETKPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
             PV+ILTAGAPPPA+VL RTESLGF+V+HGYGLTE+ G ++ C+WK EWN+ P  E+AR
Sbjct: 301 QNPVQILTAGAPPPAAVLSRTESLGFIVSHGYGLTETGGLVVSCAWKREWNKFPLAERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR L V   D++DPE+  SVKRDG+S+GEIV+RGP +MLGYLKD  ATSK + E
Sbjct: 361 LKARQGVRTLTVTEADIVDPESGLSVKRDGSSLGEIVLRGPCIMLGYLKDPNATSKCLKE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWF+TGDVGV+HPDGY+EIKDRSKDVIISGGENLSSVEVES+LY +P +NEAAVVARPD
Sbjct: 421 DGWFYTGDVGVIHPDGYMEIKDRSKDVIISGGENLSSVEVESILYTHPAINEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           E+WGETPCAF+SLK  L+RKP+E EI+EYC+ KLP +MVPKTV+F  E+PKT+TGK  KF
Sbjct: 481 EYWGETPCAFVSLKAELTRKPSEIEIMEYCRAKLPHYMVPKTVVFKDELPKTSTGKILKF 540

Query: 541 VLRQKAKSLG 551
           VLR+ AK +G
Sbjct: 541 VLREIAKGMG 545

BLAST of Csa5G318390 vs. TrEMBL
Match: B9S6X4_RICCO (AMP dependent ligase, putative OS=Ricinus communis GN=RCOM_1328840 PE=4 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 1.1e-233
Identity = 380/557 (68.22%), Postives = 469/557 (84.20%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           M+ LKP+PANSFPLTP+ F+ERA+ +YGD  SI+YN+ TYTWS+THRRCLQLASS+SS G
Sbjct: 1   MDHLKPKPANSFPLTPLSFLERASTVYGDCPSIVYNTATYTWSQTHRRCLQLASSLSSGG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
             RG VVSV APNTP MYEL FAVPM+GA+LN IN RLD RT+S+LLRHS+SKL+FVD  
Sbjct: 61  FTRGDVVSVVAPNTPPMYELQFAVPMSGAVLNNINTRLDARTVSVLLRHSQSKLVFVDCL 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S  L+ +A++LFP  +KRP LVLITDD     SS  S  S  D F  +YE+MV+ GD +F
Sbjct: 121 SRDLVLEAVSLFPSSVKRPTLVLITDDDEAPASSSTSSSSTVD-FCCTYESMVEKGDPEF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +W++P SEWDP++LNYTSGTTS+PKGVV+CHRG++ + ++S+++W VP+Q VYLWTLP+F
Sbjct: 181 KWVQPQSEWDPMILNYTSGTTSSPKGVVHCHRGIYIITVESLIDWNVPRQPVYLWTLPLF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFPWG+AAVGGTN+CIRKFD+  I+ LI+RH VTH+CGAPVVLNMLTNSPDN PL
Sbjct: 241 HANGWSFPWGMAAVGGTNICIRKFDASSIYGLIKRHGVTHMCGAPVVLNMLTNSPDNEPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
            RPV ILTAGAPPPA+VL RTESLGFVV+HGYGLTE+AG ++ C+WK +WN +PA E+AR
Sbjct: 301 KRPVHILTAGAPPPAAVLFRTESLGFVVSHGYGLTETAGLVVSCAWKPKWNTVPASERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR+     +DV+DP+T  SVKRDG S+GEIV+RG  +M+GYLKD + TSK + +
Sbjct: 361 LKARQGVRIAGFTEIDVIDPKTGLSVKRDGLSLGEIVLRGGCVMMGYLKDPDGTSKCLKD 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWF+TGDVGVMHPDGY+EIKDRSKDVIISGGEN+SSVEVESVLY NP+VNEAAVVARPD
Sbjct: 421 DGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENISSVEVESVLYSNPVVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           E+WGETPCAF+SL++G   K +E++IIEYC+ K+P +MVPKTV+F  E+PKT+TGK QKF
Sbjct: 481 EYWGETPCAFVSLRDGAREKVSEKDIIEYCREKMPHYMVPKTVVFKEELPKTSTGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLR  AK++G  + SR+
Sbjct: 541 VLRDIAKAMGASKVSRM 556

BLAST of Csa5G318390 vs. TrEMBL
Match: A0A068U0B9_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00036869001 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 7.0e-233
Identity = 379/557 (68.04%), Postives = 476/557 (85.46%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           M++LKP PANS PLTP+GF+ERAA +YGD  S++YN+ TYTWS+TH RCL++ASSI S G
Sbjct: 1   MDELKPGPANSAPLTPLGFLERAATVYGDCPSVVYNNATYTWSDTHVRCLRVASSIVSLG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I+RG VVSV APN PAMYE HFAVPMAGA+L+TINLRLD RT+SILL HSESKLI VD  
Sbjct: 61  IKRGQVVSVVAPNIPAMYEAHFAVPMAGAVLSTINLRLDARTVSILLHHSESKLILVDYQ 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S +L+ +AL+LFPP+ +RPLLVLITDD +    S+P   S   +F  +YE++V+ GD  F
Sbjct: 121 STSLVLEALSLFPPNSQRPLLVLITDDASP---SIPP--SNFPDFYCTYESLVQKGDPGF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +W+RP S++DPI+LNYTSGTTS+PKGVV+CHRG+F V +DS++EW VPK+ VYLWTLPMF
Sbjct: 181 KWVRPKSDFDPIILNYTSGTTSSPKGVVHCHRGIFIVTVDSLIEWSVPKEPVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWS+PWG+AAVGGTN+C+RKFD+ +++S IR+H+VTHLCGAPVVLNM++N P   PL
Sbjct: 241 HANGWSYPWGMAAVGGTNICLRKFDAAVVYSAIRQHNVTHLCGAPVVLNMISNFPMGEPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
             PV+I+TAGAPPPA+VLLRTE+LGF V+HGYGLTE+ G ++ C+WK++WN+LPA E+AR
Sbjct: 301 KNPVEIMTAGAPPPAAVLLRTEALGFKVSHGYGLTETGGLVVSCTWKNKWNRLPATERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR L +  +DV++PET  SVKRDG+++GE+V+RG  +MLGYLKD E TSK+  E
Sbjct: 361 LKARQGVRTLGMAEIDVIEPETGVSVKRDGSTLGELVLRGGCIMLGYLKDPEGTSKSTRE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWF+TGDV VMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYM+P VNEAAVVARPD
Sbjct: 421 NGWFYTGDVAVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMHPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SLK  + ++P E+++IE+C+GKLPK+MVPKTV+F  E+PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLKADVKQRPAEKDVIEFCRGKLPKYMVPKTVVFRQELPKTSTGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLR+ AK++G  + SRL
Sbjct: 541 VLREMAKAIGSSKVSRL 552

BLAST of Csa5G318390 vs. TAIR10
Match: AT5G16370.1 (AT5G16370.1 acyl activating enzyme 5)

HSP 1 Score: 748.0 bits (1930), Expect = 4.1e-216
Identity = 359/558 (64.34%), Postives = 452/558 (81.00%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTT-YTWSETHRRCLQLASSISSF 60
           ME +KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV I D   +++    + V+ R  F  +Y++++  GD  
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIAD---KEEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGWS+PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + +  P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+RPV ILTAGAPPPA+VLLR ES+GFV++HGYGLTE+AG  + C+WK +WN+LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+ +SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           D FWGETPCAF+SLK GL+++PTE E+IEYC+ K+PK+MVPKTV F+ E+PKT+TGK  K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQKAKSLGPPQKSRL 558
           FVLR+ AK +G  + SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of Csa5G318390 vs. TAIR10
Match: AT5G16340.1 (AT5G16340.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 744.6 bits (1921), Expect = 4.5e-215
Identity = 357/551 (64.79%), Postives = 447/551 (81.13%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTT-YTWSETHRRCLQLASSISSF 60
           ME++KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV+I D   E++  +  V  +   F  +Y+++++ GD  
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIADK--EEEGGVADVADL-SKFSYTYDDLIERGDPG 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGW+ PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + ++ P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+ PV ILTAG+PPPA+VLLR ES+GFV++HGYGLTE+AG I+ C+WK +WN LPA ++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+  SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           DEFWGETPCAF+SLK G S KPTEEE++EYC+ K+PK+MVPKTV FM E+PK++TGK  K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQKAKSLG 551
           FVLR  AK +G
Sbjct: 541 FVLRDIAKKMG 545

BLAST of Csa5G318390 vs. TAIR10
Match: AT1G75960.1 (AT1G75960.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 705.7 bits (1820), Expect = 2.3e-203
Identity = 342/551 (62.07%), Postives = 433/551 (78.58%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSISSF 60
           MEDLKP  ANS PLT +GF+ERAA +YGD TSI+Y NST YTW ET+ RCL +AS++SS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV + NTP MYEL F+VPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
               L  +A+ +    +  P+LVLI ++  E+  +    V+ R  F   Y +++  G+  
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGGA---EVTERSKFCYLYSDLITRGNPD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDPIV+NYTSGTTS+PKGVV+CHRG+F + LDS+ +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN-L 300
           FH NGW++PWGIAAVGGTNVC+RK  +  I+ LIR H VTH+ GAP+VL +L+ S ++  
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV  LTAG+ PPA+VLLR ESLGF+V+HGYGLTE+AG I+ C+WK  WN+LPA +Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAM 420
           A+LK+RQGVR +    +DV+DPE+ +SV+RDG ++GEIV+RG S+MLGYLK+   T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 SEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVAR 480
            ++GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNEAAVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQ 540
           PDEFWGETPCAF+SLK GL+RKPT++EIIEYCK K+P++M PKTV F+ E+PKT+TGK  
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLRQKAKSL 550
           K +L++ AK++
Sbjct: 541 KSLLKEIAKNM 544

BLAST of Csa5G318390 vs. TAIR10
Match: AT1G21540.1 (AT1G21540.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 686.0 bits (1769), Expect = 1.9e-197
Identity = 330/555 (59.46%), Postives = 426/555 (76.76%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTT-YTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++ + T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI RG VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSL-PSVVSIRDNFVDSYENMVKSG 180
           D  S +++ +A++ F    ++P LVL+ DD  ++D SL PS  S   +F+D+Y+ +++ G
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAAS---DFLDTYQGVMERG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWT 240
           DS+F+WIRP +EW P++LNYTSGTTS+PKGVV  HR +F + + S+L+W  P + VYLWT
Sbjct: 181 DSRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGW + WG AAVG TNVC R+ D+  I+ LI +HHVTH+C AP+VLNMLTN P 
Sbjct: 241 LPMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPS 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
             PL  PV+++TAGAPPPA+++ R E+LGF V HGYGLTE+ G ++ C+WK+EW+ L  +
Sbjct: 301 RKPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPL 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLK+RQGVR +    VDV DP T KSV+ DG S+GEIV++G S+MLGY KD E T+ 
Sbjct: 361 ERARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAA 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
            M EDGWF++GDVGV+H DGY+E+KDRSKDVII GGEN+SS EVE+VLY NP+V EAAVV
Sbjct: 421 CMREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRK--PTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTAT 540
           A+PD+ WGETPCAF+SLK   +     TE EI E+CK +LPK+MVP+ VIF  E+PKT+T
Sbjct: 481 AKPDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKFQKFVLRQKAKSL 550
           GK QKF+LRQ AKSL
Sbjct: 541 GKIQKFLLRQMAKSL 549

BLAST of Csa5G318390 vs. TAIR10
Match: AT1G21530.2 (AT1G21530.2 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 678.7 bits (1750), Expect = 3.0e-195
Identity = 329/556 (59.17%), Postives = 426/556 (76.62%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTT-YTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++ + T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI++G VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSES+L+FV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGD 180
           D  S +L+ +A++LF    K P LVL+ DD  E DSS  S      +F+D+YE +++ G+
Sbjct: 121 DHRSISLVLEAVSLFTQHEK-PHLVLLDDDQ-ENDSSSAS------DFLDTYEEIMERGN 180

Query: 181 SKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTL 240
           S+F+WIRP +EW P+VLNYTSGTTS+PKGVV  HR +F + + S+L+W VP + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTL 240

Query: 241 PMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN 300
           PMFH NGW + WG AAVG TN+C R+ D+  I++LI +H+VTH+C AP+VLNML N P +
Sbjct: 241 PMFHANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLS 300

Query: 301 LPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIE 360
            PL  PV+++T+GAPPPA+++ R ESLGF V+H YGLTE++G ++ C+WK +W+ L  +E
Sbjct: 301 TPLKNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLE 360

Query: 361 QARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKA 420
           +ARLK+RQGVR L    VDV D +T KSVK DG S+GEIV RG S+MLGY KD + T+  
Sbjct: 361 RARLKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAAC 420

Query: 421 MSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVA 480
           M EDGWF++GD+GV+H DGY+EIKDRSKDVII GGEN+SS E+E+VLY NP+V EAAVVA
Sbjct: 421 MREDGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVA 480

Query: 481 RPDEFWGETPCAFISLK---EGLSRKP-TEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTA 540
           +PD+ WGETPCAF+SLK    G    P TE EI E+CK KLPK+MVP+ VIF  E+PKT+
Sbjct: 481 KPDKMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTS 540

Query: 541 TGKFQKFVLRQKAKSL 550
           TGK QKF+LRQ AK+L
Sbjct: 541 TGKIQKFLLRQMAKTL 548

BLAST of Csa5G318390 vs. NCBI nr
Match: gi|449462358|ref|XP_004148908.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus])

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGPPQKSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of Csa5G318390 vs. NCBI nr
Match: gi|659126420|ref|XP_008463174.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 1072.8 bits (2773), Expect = 1.7e-310
Identity = 527/557 (94.61%), Postives = 541/557 (97.13%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIF+ALALFPP+MKRPLLVLI DD AEQDSSLPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTVALDS+LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFD+RLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+ LGYLKD E T+KAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           VLRQKAKSLGP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of Csa5G318390 vs. NCBI nr
Match: gi|659126423|ref|XP_008463175.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 889.0 bits (2296), Expect = 4.2e-255
Identity = 429/557 (77.02%), Postives = 491/557 (88.15%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           M+ LKP PANS PLTPI F+ERA+++Y DS SIIYNST++TWS+T+ RCL+LASS+SS G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I +GHVVSV +PNTP MYEL F+VPMAGAIL+ INLRLD RTIS+ LRHSESKL+FVDQ 
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S ALI DAL LFPP+ KRPLLVLI DD A +DS+  S V+I  +FVD YE+M++ GD +F
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSA--SSVAIGGDFVDFYEDMIQKGDPEF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDPIV+NYTSGTTS+PKGVV+ HRG+FTV LDS++EWGVPK SVYLWTLPMF
Sbjct: 181 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFP+ IAAVGGTN+CIRKFD+ LIFSLI RHHVTHLCGAPVVLNMLTNSP+N PL
Sbjct: 241 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           +RPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +I CSWK +WNQLPA E+AR
Sbjct: 301 ERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR LA+   DVLD ET KSVKRDG SIGEIV+RG S+MLGY KD EAT KAM+E
Sbjct: 361 LKARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SL+EGLSR PTEEEIIEYC+GKL  FMVPKTV+FM ++PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 558
           +LR+ AKS+GP +KSRL
Sbjct: 541 LLREMAKSMGPSRKSRL 555

BLAST of Csa5G318390 vs. NCBI nr
Match: gi|449462360|ref|XP_004148909.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus])

HSP 1 Score: 887.9 bits (2293), Expect = 9.3e-255
Identity = 432/557 (77.56%), Postives = 490/557 (87.97%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           ME LKP PANS PLTPIGF+ERA++IY DS SIIY+STT+TWS+T+RRCL+LASS+SS G
Sbjct: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I +GHVVSV APNTP MYEL FAVPMAGAIL+ INLRLD RTIS+ LRHSESKL+FVDQ 
Sbjct: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S  LI DALALFPP+ KRPLLVLI+DD A +DS+  S V+I  + VD YE+MV+ GD +F
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSA--SSVAIGGDVVDLYEDMVQKGDPEF 201

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDPIV+NYTSGTTS+PKGVV+ HRG+FTV LDS++EWGVPK SVYLWTLPMF
Sbjct: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFP+ IAAVGGTN+CIRKFD+ LIFSLI RHHVTH+CGAPVVLNMLTNSP+N PL
Sbjct: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           +RPVKILTAGAPPPA+VLLRTESLGF V+HGYGLTE+ G +I CSWK EWNQLPA E+AR
Sbjct: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR LA+   DVLD  T K V+RDG SIGEIV+RG S+MLGY KD EAT KAM+E
Sbjct: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SL+EGL+R PTEEEIIEYCKGKL  FMVPKTV+FM ++PKT+TGK QKF
Sbjct: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561

Query: 541 VLRQKAKSLGPPQKSRL 558
           +LR+ AKS+G   KSRL
Sbjct: 562 LLREMAKSMGCSPKSRL 576

BLAST of Csa5G318390 vs. NCBI nr
Match: gi|1009151390|ref|XP_015893528.1| (PREDICTED: probable acyl-activating enzyme 6 [Ziziphus jujuba])

HSP 1 Score: 826.2 bits (2133), Expect = 3.3e-236
Identity = 393/551 (71.32%), Postives = 468/551 (84.94%), Query Frame = 1

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP P NS PLTPIGF+ERAA +YGD  SI+YN+TTYTWS+TH RCL++ASSI S G
Sbjct: 1   MEDLKPTPPNSSPLTPIGFLERAATVYGDCPSIVYNTTTYTWSQTHLRCLRVASSIQSLG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I++GHVVSV APN PAMYELHFA  M+GAILN INLRLD RT+SILLRHSESKL+FVDQ 
Sbjct: 61  IKKGHVVSVLAPNIPAMYELHFAALMSGAILNNINLRLDPRTVSILLRHSESKLVFVDQL 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S +LI +AL+LFPP +  P L+LI DDT E  SS  S  S    FV SYE++V+ GD  F
Sbjct: 121 SQSLIHEALSLFPPGIPIPRLILIADDTDEASSSPSSSSSPAVEFVCSYEDLVERGDPGF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +WIRP SEWDP +LNYTSGTTS+PKGVV+C RG+F + +DS+++W VPKQ VYLWTLPMF
Sbjct: 181 EWIRPTSEWDPAILNYTSGTTSSPKGVVHCQRGLFLITVDSLIDWAVPKQPVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFPWG+AAVGGTN+C+RKFD+ +I+ LIR H+VTH+CGAPVVLNML+NSPDN PL
Sbjct: 241 HANGWSFPWGMAAVGGTNICLRKFDATIIYRLIRAHNVTHMCGAPVVLNMLSNSPDNEPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
             PV ILTAGAPPPA+VL RTESLGFVV+HGYGLTE+AG ++ C+WK EWN+LPA E+AR
Sbjct: 301 KNPVNILTAGAPPPAAVLHRTESLGFVVSHGYGLTETAGLVVSCAWKREWNRLPASERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR+  +  +DVLDP T KSVKRDG+++GEIV+RG  +MLGYLKD   T+K M +
Sbjct: 361 LKARQGVRLAGMTRIDVLDPNTGKSVKRDGSTLGEIVLRGGCVMLGYLKDPAGTAKCM-K 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDV VMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYM+P +NEAAVVARPD
Sbjct: 421 DGWFFTGDVAVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMHPAINEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGL-SRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           +FWGETPCAF+SLKE +   KP+E+EI+E+C+ KLP +MVPKTV+F  E+PKT+TGK QK
Sbjct: 481 DFWGETPCAFVSLKERIRGNKPSEKEIMEFCREKLPHYMVPKTVVFKEELPKTSTGKIQK 540

Query: 541 FVLRQKAKSLG 551
           F+LR+ AKS+G
Sbjct: 541 FILRETAKSMG 550

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AAE5_ARATH7.2e-21564.34Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana GN=AAE5 P... [more]
AAE6_ARATH8.0e-21464.79Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana GN=AAE6 PE=2 SV=1[more]
AAE8_ARATH4.1e-20262.07Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana GN=AAE8 PE=2 SV=1[more]
AEE9_ARATH3.4e-19659.46Probable acyl-activating enzyme 9 OS=Arabidopsis thaliana GN=AEE9 PE=2 SV=1[more]
AEE10_ARATH5.4e-19459.17Probable acyl-activating enzyme 10 OS=Arabidopsis thaliana GN=AEE10 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KT33_CUCSA6.5e-25577.56AMP dependent ligase OS=Cucumis sativus GN=Csa_5G317890 PE=4 SV=1[more]
A0A061ESR0_THECC2.2e-23469.64Acyl activating enzyme 5 OS=Theobroma cacao GN=TCM_020359 PE=4 SV=1[more]
A0A0D2VMX2_GOSRA8.2e-23470.18Uncharacterized protein OS=Gossypium raimondii GN=B456_011G160500 PE=4 SV=1[more]
B9S6X4_RICCO1.1e-23368.22AMP dependent ligase, putative OS=Ricinus communis GN=RCOM_1328840 PE=4 SV=1[more]
A0A068U0B9_COFCA7.0e-23368.04Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00036869001 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G16370.14.1e-21664.34 acyl activating enzyme 5[more]
AT5G16340.14.5e-21564.79 AMP-dependent synthetase and ligase family protein[more]
AT1G75960.12.3e-20362.07 AMP-dependent synthetase and ligase family protein[more]
AT1G21540.11.9e-19759.46 AMP-dependent synthetase and ligase family protein[more]
AT1G21530.23.0e-19559.17 AMP-dependent synthetase and ligase family protein[more]
Match NameE-valueIdentityDescription
gi|449462358|ref|XP_004148908.1|0.0e+00100.00PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus][more]
gi|659126420|ref|XP_008463174.1|1.7e-31094.61PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo][more]
gi|659126423|ref|XP_008463175.1|4.2e-25577.02PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo][more]
gi|449462360|ref|XP_004148909.1|9.3e-25577.56PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus][more]
gi|1009151390|ref|XP_015893528.1|3.3e-23671.32PREDICTED: probable acyl-activating enzyme 6 [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000873AMP-dep_Synth/Lig
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006744 ubiquinone biosynthetic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003987 acetate-CoA ligase activity
molecular_function GO:0008756 o-succinylbenzoate-CoA ligase activity
molecular_function GO:0003824 catalytic activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU147768cucumber EST collection version 3.0transcribed_cluster
CU156388cucumber EST collection version 3.0transcribed_cluster
CU165990cucumber EST collection version 3.0transcribed_cluster
CU168494cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa5G318390.1Csa5G318390.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU165990CU165990transcribed_cluster
CU147768CU147768transcribed_cluster
CU156388CU156388transcribed_cluster
CU168494CU168494transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 27..450
score: 3.0
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 194..205
scor
IPR025110AMP-binding enzyme C-terminal domainPFAMPF13193AMP-binding_Ccoord: 459..536
score: 3.6
NoneNo IPR availableGENE3DG3DSA:2.30.38.10coord: 375..440
score: 4.6
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 447..548
score: 5.3
NoneNo IPR availableGENE3DG3DSA:3.40.50.980coord: 204..349
score: 7.5E-29coord: 14..127
score: 1.3E-27coord: 179..203
score: 1.3
NoneNo IPR availablePANTHERPTHR24095FAMILY NOT NAMEDcoord: 1..557
score:
NoneNo IPR availablePANTHERPTHR24095:SF177ACYL-ACTIVATING ENZYME 5, PEROXISOMAL-RELATEDcoord: 1..557
score:
NoneNo IPR availableunknownSSF56801Acetyl-CoA synthetase-likecoord: 10..548
score: 4.32E

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None