Csa5G173500 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa5G173500
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Putative calcium-binding protein; contains IPR011992 (EF-hand-like domain)
Location	Chr5 : 7134570 .. 7135149 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAAACTTTCTTGTGTGTTTACCTTTCAATTCTGAAAACCAAGAACAAAAAACCATGTCTCCAACAATTAGCTCCGAAGACTTGTACAGGATCTTCAAGAAGCTAGACAAGAATGGTGATGGCCTCATTTGCCTTCAAGAGCTCAAGTGGCTCTTCGATCGAGTTGGGGTCGAATTAACCATGGAGGAGTTGGAGTCTTTTCTAGAGAAACCGAGCCTTGACTTCGACCAGTTCTTGTTCTGTTACAAGTCCATATCCAAACAAAACAAAGGTGAGTGCGAAGAGGAGGATGTACTGGGGTGTCTGGAGGAAGATGATCATGAGGAAGATATGGAGATGATATGTATGGCTTTTAAAGTGTTTGAAATGAGTGACGATGATGGCTTCATTTCTTGCGATGGACTCGAAAACGTGTTGGCAAGGCTTAATGAGTATGACCAACGTCTTCAACAATCTGAAAGACAAAGAAAAGTTGAAGTACAAGCACTCAAGCATCAAATTATGGAATTAGAAAACCGTTTTGAGGATAGATTTAAAACTATAATGACAGAGATGCATAAGCGTCATGTTTCGAGTTGA

mRNA sequence

ATGTCTCCAACAATTAGCTCCGAAGACTTGTACAGGATCTTCAAGAAGCTAGACAAGAATGGTGATGGCCTCATTTGCCTTCAAGAGCTCAAGTGGCTCTTCGATCGAGTTGGGGTCGAATTAACCATGGAGGAGTTGGAGTCTTTTCTAGAGAAACCGAGCCTTGACTTCGACCAGTTCTTGTTCTGTTACAAGTCCATATCCAAACAAAACAAAGGTGAGTGCGAAGAGGAGGATGTACTGGGGTGTCTGGAGGAAGATGATCATGAGGAAGATATGGAGATGATATGTATGGCTTTTAAAGTGTTTGAAATGAGTGACGATGATGGCTTCATTTCTTGCGATGGACTCGAAAACGTGTTGGCAAGGCTTAATGAGTATGACCAACGTCTTCAACAATCTGAAAGACAAAGAAAAGTTGAAGTACAAGCACTCAAGCATCAAATTATGGAATTAGAAAACCGTTTTGAGGATAGATTTAAAACTATAATGACAGAGATGCATAAGCGTCATGTTTCGAGTTGA

Coding sequence (CDS)

ATGTCTCCAACAATTAGCTCCGAAGACTTGTACAGGATCTTCAAGAAGCTAGACAAGAATGGTGATGGCCTCATTTGCCTTCAAGAGCTCAAGTGGCTCTTCGATCGAGTTGGGGTCGAATTAACCATGGAGGAGTTGGAGTCTTTTCTAGAGAAACCGAGCCTTGACTTCGACCAGTTCTTGTTCTGTTACAAGTCCATATCCAAACAAAACAAAGGTGAGTGCGAAGAGGAGGATGTACTGGGGTGTCTGGAGGAAGATGATCATGAGGAAGATATGGAGATGATATGTATGGCTTTTAAAGTGTTTGAAATGAGTGACGATGATGGCTTCATTTCTTGCGATGGACTCGAAAACGTGTTGGCAAGGCTTAATGAGTATGACCAACGTCTTCAACAATCTGAAAGACAAAGAAAAGTTGAAGTACAAGCACTCAAGCATCAAATTATGGAATTAGAAAACCGTTTTGAGGATAGATTTAAAACTATAATGACAGAGATGCATAAGCGTCATGTTTCGAGTTGA

Protein sequence

MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQFLFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENVLARLNEYDQRLQQSERQRKVEVQALKHQIMELENRFEDRFKTIMTEMHKRHVSS*

Homology

[Close]

BLAST of Csa5G173500 vs. Swiss-Prot
Match: CML44_ARATH (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana GN=CML44 PE=2 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 3.1e-15
Identity = 51/121 (42.15%), Postives = 71/121 (58.68%), Query Frame = 1


Query: 5   ISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVG-VELTMEELESFLEKPSLDFDQFLFC 64
           I++ DL R+FK LDKN DGL+ L EL W+ D++G  E T +ELE  + K SLD D+FL  
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 65  YKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENVLAR 124
           Y      +KG  +  DV+         ++ E I  AF VF++ + DG+IS + L +VL R
Sbjct: 66  YYDAVLDSKGSKKNIDVVA--------DNDEAIARAFNVFDV-NGDGYISAEELRDVLER 117

[Close]

BLAST of Csa5G173500 vs. Swiss-Prot
Match: CML42_ARATH (Calcium-binding protein CML42 OS=Arabidopsis thaliana GN=CML42 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 7.5e-09
Identity = 45/135 (33.33%), Postives = 65/135 (48.15%), Query Frame = 1


Query: 2   SPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLE------KPSL 61
           SP++++  L RIF   DKNGDG I ++EL     R+G+   + +L+S +E         L
Sbjct: 22  SPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGL 81

Query: 62  DFDQFLFCYKSISKQ------NKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDD 121
           +FD F   +K++           GE E++       E D  E       AFKVF+  + D
Sbjct: 82  NFDDFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAE-------AFKVFD-ENGD 141

Query: 122 GFISCDGLENVLARL 125
           GFIS   L+ VL +L
Sbjct: 142 GFISARELQTVLKKL 148

[Close]

BLAST of Csa5G173500 vs. Swiss-Prot
Match: ALLB3_BETPN (Calcium-binding allergen Bet v 3 OS=Betula pendula GN=BETVIII PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.2e-08
Identity = 44/138 (31.88%), Postives = 69/138 (50.00%), Query Frame = 1


Query: 2   SPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLE------KPSL 61
           S ++++  L RIF   DKN DG+I + EL    + +G+E  + ELES ++         L
Sbjct: 33  SESLNTLRLRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGL 92

Query: 62  DFDQFLFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMA---------FKVFEMS 121
            F+ F+  ++S++       +     G  +EDD+EEDM    ++         FKVF+  
Sbjct: 93  QFEDFISLHQSLN-------DSYFAYGGEDEDDNEEDMRKSILSQEEADSFGGFKVFD-E 152

Query: 122 DDDGFISCDGLENVLARL 125
           D DG+IS   L+ VL +L
Sbjct: 153 DGDGYISARELQMVLGKL 162

[Close]

BLAST of Csa5G173500 vs. Swiss-Prot
Match: CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.8e-08
Identity = 43/132 (32.58%), Postives = 66/132 (50.00%), Query Frame = 1


Query: 2   SPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFL------EKPSL 61
           SP++++  L+R+F   DKN DG I ++EL     R+G++    +L+S +      +K  L
Sbjct: 21  SPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGL 80

Query: 62  DFDQFLFCYKSISKQ---NKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFI 121
            FD F   +K++ +     +G C       C  +   E D+E    AF VF+  D DGFI
Sbjct: 81  RFDDFAALHKTLDESFFGGEGSC-------C--DGSPESDLE---EAFNVFD-EDGDGFI 139

Query: 122 SCDGLENVLARL 125
           S   L+ VL +L
Sbjct: 141 SAVELQKVLKKL 139

[Close]

BLAST of Csa5G173500 vs. Swiss-Prot
Match: CML7_ARATH (Calmodulin-like protein 7 OS=Arabidopsis thaliana GN=CML7 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 8.3e-08
Identity = 46/130 (35.38%), Postives = 63/130 (48.46%), Query Frame = 1


Query: 1   MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEK------PS 60
           M PT    +L R+F+  DKNGDG I  +EL      +G+ +  +EL   +EK        
Sbjct: 1   MDPT----ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGC 60

Query: 61  LDFDQFLFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISC 120
           +D D+F   YK+I                 EED+ EEDM+    AF VF+  + DGFI+ 
Sbjct: 61  VDIDEFGELYKTIMD---------------EEDEEEEDMK---EAFNVFD-QNGDGFITV 107

Query: 121 DGLENVLARL 125
           D L+ VL+ L
Sbjct: 121 DELKAVLSSL 107

HSP 2 Score: 36.6 bits (83), Expect = 3.4e-01
Identity = 21/68 (30.88%), Postives = 35/68 (51.47%), Query Frame = 1


Query: 8   EDLYRIFKKLDKNGDGLICLQELKWLFDRVGVE--LTMEELESFLEKPSLDFDQFLFCYK 67
           ED+   F   D+NGDG I + ELK +   +G++   T+++ +  ++K  +D D  +  YK
Sbjct: 78  EDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRV-NYK 137

Query: 68  SISKQNKG 74
              +  KG
Sbjct: 138 EFRQMMKG 144

[Close]

BLAST of Csa5G173500 vs. TrEMBL
Match: A0A0A0KLD7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G173500 PE=4 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 3.6e-95
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 1


Query: 1   MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQF 60
           MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQF
Sbjct: 1   MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQF 60

Query: 61  LFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENV 120
           LFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENV
Sbjct: 61  LFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENV 120

Query: 121 LARLNEYDQRLQQSERQRKVEVQALKHQIMELENRFEDRFKTIMTEMHKRHVSS 175
           LARLNEYDQRLQQSERQRKVEVQALKHQIMELENRFEDRFKTIMTEMHKRHVSS
Sbjct: 121 LARLNEYDQRLQQSERQRKVEVQALKHQIMELENRFEDRFKTIMTEMHKRHVSS 174

[Close]

BLAST of Csa5G173500 vs. TrEMBL
Match: A0A0A0KFQ6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.0e-40
Identity = 86/129 (66.67%), Postives = 109/129 (84.50%), Query Frame = 1


Query: 3   PTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQFLF 62
           P ISS+DL+RIFKKLDKN DGLICLQEL WL D +G++LTMEELESFLE+PSLDFD+FLF
Sbjct: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFLF 61

Query: 63  CYKSISKQNKGECEEEDVLGCLEEDDH---EEDMEMICMAFKVFEMSDDDGFISCDGLEN 122
            Y+SISKQNKGEC +  V GC++++D+   ++DME++ +AFKVF+M + DGFISCD LEN
Sbjct: 62  FYESISKQNKGEC-KGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDM-NGDGFISCDELEN 121

Query: 123 VLARLNEYD 129
           VL +L  +D
Sbjct: 122 VLVKLELWD 128

[Close]

BLAST of Csa5G173500 vs. TrEMBL
Match: A0A067GL51_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g044803mg PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 8.0e-26
Identity = 63/130 (48.46%), Postives = 93/130 (71.54%), Query Frame = 1


Query: 5   ISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQFLFCY 64
           + + DL RIFK+LDKNGD  + L+EL WL +R+GV L++EELESF+ K SLD ++FLF +
Sbjct: 4   LHTADLQRIFKQLDKNGDDQVSLEELNWLLERIGVRLSLEELESFVGKSSLDLNEFLFFW 63

Query: 65  KSISKQNKGECEEEDVLGCLEEDDHEED----MEMICMAFKVFEMSDDDGFISCDGLENV 124
           KSISKQN  + ++ +V   +  DD+E D    +  +  AF VF++ + DGFISC+ L+NV
Sbjct: 64  KSISKQNNNKVDDHEVKDEINGDDNENDDDGEISDLLEAFNVFDL-NGDGFISCEELQNV 123

Query: 125 LARLNEYDQR 131
           L+RL  +D++
Sbjct: 124 LSRLGLWDEK 132

[Close]

BLAST of Csa5G173500 vs. TrEMBL
Match: V4TCZ3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002706mg PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 8.0e-26
Identity = 63/130 (48.46%), Postives = 93/130 (71.54%), Query Frame = 1


Query: 5   ISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQFLFCY 64
           + + DL RIFK+LDKNGD  + L+EL WL +R+GV L++EELESF+ K SLD ++FLF +
Sbjct: 4   LHTADLQRIFKQLDKNGDDQVSLEELNWLLERIGVRLSLEELESFVGKSSLDLNEFLFFW 63

Query: 65  KSISKQNKGECEEEDVLGCLEEDDHEED----MEMICMAFKVFEMSDDDGFISCDGLENV 124
           KSISKQN  + ++ +V   +  DD+E D    +  +  AF VF++ + DGFISC+ L+NV
Sbjct: 64  KSISKQNNNKVDDHEVKDEINGDDNENDDDGEISDLLEAFNVFDL-NGDGFISCEELQNV 123

Query: 125 LARLNEYDQR 131
           L+RL  +D++
Sbjct: 124 LSRLGLWDEK 132

[Close]

BLAST of Csa5G173500 vs. TrEMBL
Match: F6H0H4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g01630 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.4e-25
Identity = 62/128 (48.44%), Postives = 96/128 (75.00%), Query Frame = 1


Query: 2   SPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQFL 61
           S ++SS DL+RIF KLD+NGDGL+ L EL WL +RVGV+ +++ELES +   +LDF++FL
Sbjct: 3   SSSLSSIDLHRIFHKLDRNGDGLVSLGELNWLLERVGVQYSLDELESLVGNTTLDFNEFL 62

Query: 62  FCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENVL 121
             Y+SIS + KGE E E+    ++E++ EED+  +  AF+VF++ + DGFI+CD L++VL
Sbjct: 63  VFYESISTEKKGESEVEEEE--VKEEEEEEDVGDLAKAFRVFDL-NGDGFITCDELQSVL 122

Query: 122 ARLNEYDQ 130
           +RL  +++
Sbjct: 123 SRLGMWEE 127

[Close]

BLAST of Csa5G173500 vs. TAIR10
Match: AT1G21550.1 (AT1G21550.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 83.2 bits (204), Expect = 1.8e-16
Identity = 51/121 (42.15%), Postives = 71/121 (58.68%), Query Frame = 1


Query: 5   ISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVG-VELTMEELESFLEKPSLDFDQFLFC 64
           I++ DL R+FK LDKN DGL+ L EL W+ D++G  E T +ELE  + K SLD D+FL  
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 65  YKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENVLAR 124
           Y      +KG  +  DV+         ++ E I  AF VF++ + DG+IS + L +VL R
Sbjct: 66  YYDAVLDSKGSKKNIDVVA--------DNDEAIARAFNVFDV-NGDGYISAEELRDVLER 117

[Close]

BLAST of Csa5G173500 vs. TAIR10
Match: AT4G20780.1 (AT4G20780.1 calmodulin like 42)

HSP 1 Score: 62.0 bits (149), Expect = 4.2e-10
Identity = 45/135 (33.33%), Postives = 65/135 (48.15%), Query Frame = 1


Query: 2   SPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLE------KPSL 61
           SP++++  L RIF   DKNGDG I ++EL     R+G+   + +L+S +E         L
Sbjct: 22  SPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGL 81

Query: 62  DFDQFLFCYKSISKQ------NKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDD 121
           +FD F   +K++           GE E++       E D  E       AFKVF+  + D
Sbjct: 82  NFDDFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAE-------AFKVFD-ENGD 141

Query: 122 GFISCDGLENVLARL 125
           GFIS   L+ VL +L
Sbjct: 142 GFISARELQTVLKKL 148

[Close]

BLAST of Csa5G173500 vs. TAIR10
Match: AT5G44460.1 (AT5G44460.1 calmodulin like 43)

HSP 1 Score: 60.1 bits (144), Expect = 1.6e-09
Identity = 43/132 (32.58%), Postives = 66/132 (50.00%), Query Frame = 1


Query: 2   SPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFL------EKPSL 61
           SP++++  L+R+F   DKN DG I ++EL     R+G++    +L+S +      +K  L
Sbjct: 21  SPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGL 80

Query: 62  DFDQFLFCYKSISKQ---NKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFI 121
            FD F   +K++ +     +G C       C  +   E D+E    AF VF+  D DGFI
Sbjct: 81  RFDDFAALHKTLDESFFGGEGSC-------C--DGSPESDLE---EAFNVFD-EDGDGFI 139

Query: 122 SCDGLENVLARL 125
           S   L+ VL +L
Sbjct: 141 SAVELQKVLKKL 139

[Close]

BLAST of Csa5G173500 vs. TAIR10
Match: AT2G43290.1 (AT2G43290.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 58.5 bits (140), Expect = 4.7e-09
Identity = 42/128 (32.81%), Postives = 60/128 (46.88%), Query Frame = 1


Query: 5   ISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEK------PSLDFD 64
           I   +L R+F+  DKNGDG I  +EL    + +G+ +  ++L   + K        +D D
Sbjct: 61  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 120

Query: 65  QFLFCYKSI--SKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDG 124
           +F   Y SI     N GE EEED+                  AF VF+  D DGFI+ + 
Sbjct: 121 EFESLYSSIVDEHHNDGETEEEDMKD----------------AFNVFD-QDGDGFITVEE 171

[Close]

BLAST of Csa5G173500 vs. TAIR10
Match: AT1G05990.1 (AT1G05990.1 EF hand calcium-binding protein family)

HSP 1 Score: 58.5 bits (140), Expect = 4.7e-09
Identity = 46/130 (35.38%), Postives = 63/130 (48.46%), Query Frame = 1


Query: 1   MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEK------PS 60
           M PT    +L R+F+  DKNGDG I  +EL      +G+ +  +EL   +EK        
Sbjct: 1   MDPT----ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGC 60

Query: 61  LDFDQFLFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISC 120
           +D D+F   YK+I                 EED+ EEDM+    AF VF+  + DGFI+ 
Sbjct: 61  VDIDEFGELYKTIMD---------------EEDEEEEDMK---EAFNVFD-QNGDGFITV 107

Query: 121 DGLENVLARL 125
           D L+ VL+ L
Sbjct: 121 DELKAVLSSL 107

HSP 2 Score: 36.6 bits (83), Expect = 1.9e-02
Identity = 21/68 (30.88%), Postives = 35/68 (51.47%), Query Frame = 1


Query: 8   EDLYRIFKKLDKNGDGLICLQELKWLFDRVGVE--LTMEELESFLEKPSLDFDQFLFCYK 67
           ED+   F   D+NGDG I + ELK +   +G++   T+++ +  ++K  +D D  +  YK
Sbjct: 78  EDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRV-NYK 137

Query: 68  SISKQNKG 74
              +  KG
Sbjct: 138 EFRQMMKG 144

[Close]

BLAST of Csa5G173500 vs. NCBI nr
Match: gi|778700636|ref|XP_004143706.2| (PREDICTED: probable calcium-binding protein CML44 [Cucumis sativus])

HSP 1 Score: 355.5 bits (911), Expect = 5.2e-95
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 1


Query: 1   MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQF 60
           MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQF
Sbjct: 1   MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQF 60

Query: 61  LFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENV 120
           LFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENV
Sbjct: 61  LFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENV 120

Query: 121 LARLNEYDQRLQQSERQRKVEVQALKHQIMELENRFEDRFKTIMTEMHKRHVSS 175
           LARLNEYDQRLQQSERQRKVEVQALKHQIMELENRFEDRFKTIMTEMHKRHVSS
Sbjct: 121 LARLNEYDQRLQQSERQRKVEVQALKHQIMELENRFEDRFKTIMTEMHKRHVSS 174

[Close]

BLAST of Csa5G173500 vs. NCBI nr
Match: gi|659081990|ref|XP_008441608.1| (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo])

HSP 1 Score: 183.3 bits (464), Expect = 3.5e-43
Identity = 94/128 (73.44%), Postives = 111/128 (86.72%), Query Frame = 1


Query: 1   MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQF 60
           MSP ISS+DL+RIFKKLDKN DGL+CLQELKWL DR+G++LTMEELESFLEKPSLDFD+F
Sbjct: 1   MSP-ISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENV 120
           LF Y+SISKQNKGEC +  V GC+ +DDHE+D E++ MAFKVF+M + DGFISCD LENV
Sbjct: 61  LFFYESISKQNKGEC-KGGVAGCV-QDDHEDDTEIVYMAFKVFDM-NGDGFISCDELENV 120

Query: 121 LARLNEYD 129
           L RL  +D
Sbjct: 121 LVRLELWD 124

[Close]

BLAST of Csa5G173500 vs. NCBI nr
Match: gi|449463432|ref|XP_004149438.1| (PREDICTED: probable calcium-binding protein CML44 [Cucumis sativus])

HSP 1 Score: 173.7 bits (439), Expect = 2.8e-40
Identity = 86/129 (66.67%), Postives = 109/129 (84.50%), Query Frame = 1


Query: 3   PTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQFLF 62
           P ISS+DL+RIFKKLDKN DGLICLQEL WL D +G++LTMEELESFLE+PSLDFD+FLF
Sbjct: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFLF 61

Query: 63  CYKSISKQNKGECEEEDVLGCLEEDDH---EEDMEMICMAFKVFEMSDDDGFISCDGLEN 122
            Y+SISKQNKGEC +  V GC++++D+   ++DME++ +AFKVF+M + DGFISCD LEN
Sbjct: 62  FYESISKQNKGEC-KGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDM-NGDGFISCDELEN 121

Query: 123 VLARLNEYD 129
           VL +L  +D
Sbjct: 122 VLVKLELWD 128

[Close]

BLAST of Csa5G173500 vs. NCBI nr
Match: gi|568837720|ref|XP_006472871.1| (PREDICTED: probable calcium-binding protein CML44 [Citrus sinensis])

HSP 1 Score: 127.9 bits (320), Expect = 1.8e-26
Identity = 64/130 (49.23%), Postives = 94/130 (72.31%), Query Frame = 1


Query: 5   ISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQFLFCY 64
           + + DL RIFK+LDKNGD  + L+EL WL +R+GV L++EELESF+ K SLD ++FLF +
Sbjct: 4   LHTADLQRIFKQLDKNGDDQVSLEELNWLLERIGVRLSLEELESFVGKSSLDLNEFLFFW 63

Query: 65  KSISKQNKGECEEEDVLGCLEEDDHEED----MEMICMAFKVFEMSDDDGFISCDGLENV 124
           KSISKQN  + ++ +V G +  DD+E D    +  +  AF VF++ + DGFISC+ L+NV
Sbjct: 64  KSISKQNNNKVDDHEVKGEINGDDNENDDDGEISDLLEAFNVFDL-NGDGFISCEELQNV 123

Query: 125 LARLNEYDQR 131
           L+RL  +D++
Sbjct: 124 LSRLGLWDEK 132

[Close]

BLAST of Csa5G173500 vs. NCBI nr
Match: gi|641861766|gb|KDO80453.1| (hypothetical protein CISIN_1g044803mg, partial [Citrus sinensis])

HSP 1 Score: 125.2 bits (313), Expect = 1.1e-25
Identity = 63/130 (48.46%), Postives = 93/130 (71.54%), Query Frame = 1


Query: 5   ISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQFLFCY 64
           + + DL RIFK+LDKNGD  + L+EL WL +R+GV L++EELESF+ K SLD ++FLF +
Sbjct: 4   LHTADLQRIFKQLDKNGDDQVSLEELNWLLERIGVRLSLEELESFVGKSSLDLNEFLFFW 63

Query: 65  KSISKQNKGECEEEDVLGCLEEDDHEED----MEMICMAFKVFEMSDDDGFISCDGLENV 124
           KSISKQN  + ++ +V   +  DD+E D    +  +  AF VF++ + DGFISC+ L+NV
Sbjct: 64  KSISKQNNNKVDDHEVKDEINGDDNENDDDGEISDLLEAFNVFDL-NGDGFISCEELQNV 123

Query: 125 LARLNEYDQR 131
           L+RL  +D++
Sbjct: 124 LSRLGLWDEK 132

The following BLAST results are available for this feature:

BLAST of Csa5G173500 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML44_ARATH	3.1e-15	42.15	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana GN=CML44 PE=2 SV=...	[more]
CML42_ARATH	7.5e-09	33.33	Calcium-binding protein CML42 OS=Arabidopsis thaliana GN=CML42 PE=1 SV=1	[more]
ALLB3_BETPN	2.2e-08	31.88	Calcium-binding allergen Bet v 3 OS=Betula pendula GN=BETVIII PE=1 SV=1	[more]
CML43_ARATH	2.8e-08	32.58	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=...	[more]
CML7_ARATH	8.3e-08	35.38	Calmodulin-like protein 7 OS=Arabidopsis thaliana GN=CML7 PE=2 SV=1	[more]

BLAST of Csa5G173500 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLD7_CUCSA	3.6e-95	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G173500 PE=4 SV=1	[more]
A0A0A0KFQ6_CUCSA	2.0e-40	66.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G376250 PE=4 SV=1	[more]
A0A067GL51_CITSI	8.0e-26	48.46	Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g044803mg PE=4 S...	[more]
V4TCZ3_9ROSI	8.0e-26	48.46	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002706mg PE=4 SV=1	[more]
F6H0H4_VITVI	1.4e-25	48.44	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g01630 PE=4 SV=...	[more]

BLAST of Csa5G173500 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G21550.1	1.8e-16	42.15	Calcium-binding EF-hand family protein	[more]
AT4G20780.1	4.2e-10	33.33	calmodulin like 42	[more]
AT5G44460.1	1.6e-09	32.58	calmodulin like 43	[more]
AT2G43290.1	4.7e-09	32.81	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	4.7e-09	35.38	EF hand calcium-binding protein family	[more]

BLAST of Csa5G173500 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778700636\|ref\|XP_004143706.2\|	5.2e-95	100.00	PREDICTED: probable calcium-binding protein CML44 [Cucumis sativus]	[more]
gi\|659081990\|ref\|XP_008441608.1\|	3.5e-43	73.44	PREDICTED: probable calcium-binding protein CML44 [Cucumis melo]	[more]
gi\|449463432\|ref\|XP_004149438.1\|	2.8e-40	66.67	PREDICTED: probable calcium-binding protein CML44 [Cucumis sativus]	[more]
gi\|568837720\|ref\|XP_006472871.1\|	1.8e-26	49.23	PREDICTED: probable calcium-binding protein CML44 [Citrus sinensis]	[more]
gi\|641861766\|gb\|KDO80453.1\|	1.1e-25	48.46	hypothetical protein CISIN_1g044803mg, partial [Citrus sinensis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa5G173500.1	Csa5G173500.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 9..37 score: 0
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 5..40 score: 13
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 8..126 score: 7.5
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 8..125 score: 3.06
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 18..30 scor
None	No IPR available	unknown	Coil	Coil	coord: 117..155 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 4..79 score: 2.7E-23coord: 96..124 score: 2.7
None	No IPR available	PANTHER	PTHR10891:SF530	SUBFAMILY NOT NAMED	coord: 4..79 score: 2.7E-23coord: 96..124 score: 2.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa5G173500	CSPI05G08420	Wild cucumber (PI 183967)	cpicuB240
Csa5G173500	Cucsa.166410	Cucumber (Gy14) v1	cgycuB251
Csa5G173500	CsGy5G005810	Cucumber (Gy14) v2	cgybcuB224

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa5G173500	Cucumber (Gy14) v1	cgycuB370
Csa5G173500	Cucurbita maxima (Rimu)	cmacuB291
Csa5G173500	Cucurbita maxima (Rimu)	cmacuB609
Csa5G173500	Cucurbita moschata (Rifu)	cmocuB278
Csa5G173500	Cucurbita moschata (Rifu)	cmocuB599
Csa5G173500	Melon (DHL92) v3.5.1	cumeB403
Csa5G173500	Melon (DHL92) v3.5.1	cumeB359
Csa5G173500	Watermelon (Charleston Gray)	cuwcgB364
Csa5G173500	Watermelon (97103) v1	cuwmB400
Csa5G173500	Watermelon (97103) v1	cuwmB411
Csa5G173500	Cucurbita pepo (Zucchini)	cpecuB186
Csa5G173500	Cucurbita pepo (Zucchini)	cpecuB737
Csa5G173500	Bottle gourd (USVL1VR-Ls)	culsiB322
Csa5G173500	Bottle gourd (USVL1VR-Ls)	culsiB380
Csa5G173500	Cucumber (Gy14) v2	cgybcuB127
Csa5G173500	Melon (DHL92) v3.6.1	cumedB339
Csa5G173500	Melon (DHL92) v3.6.1	cumedB387
Csa5G173500	Silver-seed gourd	carcuB0733
Csa5G173500	Silver-seed gourd	carcuB0756
Csa5G173500	Watermelon (97103) v2	cuwmbB336
Csa5G173500	Wax gourd	cuwgoB456