Csa5G161940 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa5G161940
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	L-lactate dehydrogenase; contains IPR001557 (L-lactate/malate dehydrogenase)
Location	Chr5 : 6070574 .. 6071104 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAAAAGTTCCTCAATCTCTTCCTTAGGTCCCGGCGGCCTCGACCTTTCTCAAGCCTTCTTCAAACCAATTCGTGACGCCTCTCCTCCATCCACAGCAAATCGCAACACCAAAATCTCCGTCATCGGCGCCGGAAACGTTGGTATGGCCATCGCTCAAACCATCCTCACTCAAGATCTGGTCGACGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGTGGCGAAATGCTAGATCTCCAACATGCTGCTGCTTTTCTCCCTCGTACAAAAATTAGCGCCTCCACTGATTACTCCATTACTGCTGGCTCTGATCTGTGTATTGTTACAGGTGGTGCTCGGCAGATCGCCGGTGAATCTAGGTTGAATCTGTTACAGAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGCCGTTGGTTCAGTTCTCTCCTGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATATTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGTGTGATTTGA

mRNA sequence

ATGCAGAAAAGTTCCTCAATCTCTTCCTTAGGTCCCGGCGGCCTCGACCTTTCTCAAGCCTTCTTCAAACCAATTCGTGACGCCTCTCCTCCATCCACAGCAAATCGCAACACCAAAATCTCCGTCATCGGCGCCGGAAACGTTGGTATGGCCATCGCTCAAACCATCCTCACTCAAGATCTGGTCGACGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGTGGCGAAATGCTAGATCTCCAACATGCTGCTGCTTTTCTCCCTCGTACAAAAATTAGCGCCTCCACTGATTACTCCATTACTGCTGGCTCTGATCTGTGTATTGTTACAGGTGGTGCTCGGCAGATCGCCGGTGAATCTAGGTTGAATCTGTTACAGAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGCCGTTGGTTCAGTTCTCTCCTGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATATTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGTGTGATTTGA

Coding sequence (CDS)

ATGCAGAAAAGTTCCTCAATCTCTTCCTTAGGTCCCGGCGGCCTCGACCTTTCTCAAGCCTTCTTCAAACCAATTCGTGACGCCTCTCCTCCATCCACAGCAAATCGCAACACCAAAATCTCCGTCATCGGCGCCGGAAACGTTGGTATGGCCATCGCTCAAACCATCCTCACTCAAGATCTGGTCGACGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGTGGCGAAATGCTAGATCTCCAACATGCTGCTGCTTTTCTCCCTCGTACAAAAATTAGCGCCTCCACTGATTACTCCATTACTGCTGGCTCTGATCTGTGTATTGTTACAGGTGGTGCTCGGCAGATCGCCGGTGAATCTAGGTTGAATCTGTTACAGAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGCCGTTGGTTCAGTTCTCTCCTGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATATTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGTGTGATTTGA

Protein sequence

MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI*

Homology

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BLAST of Csa5G161940 vs. Swiss-Prot
Match: LDH_MAIZE (L-lactate dehydrogenase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.3e-61
Identity = 123/180 (68.33%), Postives = 148/180 (82.22%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIR-DASPPSTAN---RNTKISVIGAGNVGMAIAQTI 60
           M+K++S+S LG    D S  FF+P+  D+S P++ +   R TK+SVIGAGNVGMAIAQTI
Sbjct: 1   MKKATSLSELGFDAGDASSGFFRPVSGDSSTPTSQHHRRRLTKVSVIGAGNVGMAIAQTI 60

Query: 61  LTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGAR 120
           LT+DL DE+ LVDA PDKLRGEMLDLQHAAAFLPRT++ + TD S+T GSDL IVT GAR
Sbjct: 61  LTRDLADEIALVDAVPDKLRGEMLDLQHAAAFLPRTRLVSGTDMSVTRGSDLVIVTAGAR 120

Query: 121 QIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           QI GE+RL+LLQRNVALF+KIVPPL + S + +LL+VSNPVDVLTY+AWKLSGFP++RVI
Sbjct: 121 QIQGETRLDLLQRNVALFRKIVPPLAEQSHDALLLVVSNPVDVLTYVAWKLSGFPASRVI 180

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BLAST of Csa5G161940 vs. Swiss-Prot
Match: LDHA_HORVU (L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 9.4e-60
Identity = 124/182 (68.13%), Postives = 144/182 (79.12%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRD---ASPPSTA---NRNTKISVIGAGNVGMAIAQ 60
           M K+SS+S LG      S  FF+P+ D   A+P S+A    R TKISVIGAGNVGMAIAQ
Sbjct: 1   MHKASSLSELGFDAGGASSGFFRPVADGCPATPTSSAVPHRRLTKISVIGAGNVGMAIAQ 60

Query: 61  TILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGG 120
           TILTQ+L DE+ LVDA PDKLRGE LDLQHAAAFLPR +IS  TD ++T  SDL IVT G
Sbjct: 61  TILTQNLADEIALVDALPDKLRGEALDLQHAAAFLPRVRISG-TDAAVTKNSDLVIVTAG 120

Query: 121 ARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNR 177
           ARQI GE+RLNLLQRNVAL++KIVPP+ + SP+ +LL+VSNPVDVLTY+AWKLSGFP++R
Sbjct: 121 ARQIPGETRLNLLQRNVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASR 180

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BLAST of Csa5G161940 vs. Swiss-Prot
Match: LDHB_HORVU (L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.7e-56
Identity = 111/167 (66.47%), Postives = 132/167 (79.04%), Query Frame = 1


Query: 16  DLSQAFFKPIRDASPPSTAN------RNTKISVIGAGNVGMAIAQTILTQDLVDELVLVD 75
           D S  FF+ + D  P +  +      R TK+SVIGAGNVGMAIAQTILTQ+L DE+ LVD
Sbjct: 3   DASSGFFRAVADGCPITHTSCSAPHRRLTKVSVIGAGNVGMAIAQTILTQNLADEIALVD 62

Query: 76  AKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAGESRLNLLQR 135
           A PDKLRGE LDLQHAAAFLPR +I + TD ++T  SDL +VT GARQI GE+RLNLLQR
Sbjct: 63  ALPDKLRGEALDLQHAAAFLPRVRIVSGTDAAVTKNSDLIVVTAGARQIPGETRLNLLQR 122

Query: 136 NVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           NVAL++KIVPP+ + SP+ +LL+VSNPVDVLTY+AWKLSGFP++RVI
Sbjct: 123 NVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASRVI 169

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BLAST of Csa5G161940 vs. Swiss-Prot
Match: LDHA_CHICK (L-lactate dehydrogenase A chain OS=Gallus gallus GN=LDHA PE=1 SV=3)

HSP 1 Score: 177.2 bits (448), Expect = 1.6e-43
Identity = 90/143 (62.94%), Postives = 109/143 (76.22%), Query Frame = 1


Query: 34  ANRNTKISVIGAGNVGMAIAQTILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTK 93
           A+ + KISV+G G VGMA A +IL +DL DEL LVD   DKL+GEMLDLQH + FL   K
Sbjct: 17  AHAHNKISVVGVGAVGMACAISILMKDLADELTLVDVVEDKLKGEMLDLQHGSLFLKTPK 76

Query: 94  ISASTDYSITAGSDLCIVTGGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIV 153
           I +  DYS+TA S L IVT GARQ  GESRLNL+QRNV +F+ I+P +V++SP+  LLIV
Sbjct: 77  IISGKDYSVTAHSKLVIVTAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPDCKLLIV 136

Query: 154 SNPVDVLTYIAWKLSGFPSNRVI 177
           SNPVD+LTY+AWK+SGFP +RVI
Sbjct: 137 SNPVDILTYVAWKISGFPKHRVI 159

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BLAST of Csa5G161940 vs. Swiss-Prot
Match: LDH_CAEEL (L-lactate dehydrogenase OS=Caenorhabditis elegans GN=ldh-1 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.6e-43
Identity = 85/141 (60.28%), Postives = 106/141 (75.18%), Query Frame = 1


Query: 35  NRNTKISVIGAGNVGMAIAQTILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKI 94
           N + K++V+G G VGMA A +IL Q+L +EL LVD   DKL+GEM+DLQH  AF     +
Sbjct: 18  NSHGKVTVVGVGQVGMACAYSILQQNLANELCLVDVVADKLKGEMMDLQHGLAFTRHCTV 77

Query: 95  SASTDYSITAGSDLCIVTGGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVS 154
            A TDYSITAGS LC+VT GARQ  GE+RL+L+QRNV +F+ I+P LV++SP+T +L+VS
Sbjct: 78  KADTDYSITAGSKLCVVTAGARQREGETRLSLVQRNVEIFKGIIPQLVKYSPDTCILVVS 137

Query: 155 NPVDVLTYIAWKLSGFPSNRV 176
           NPVDVLTY+ WKLSG P  RV
Sbjct: 138 NPVDVLTYVTWKLSGLPRERV 158

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BLAST of Csa5G161940 vs. TrEMBL
Match: A0A0A0KKM5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G161940 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 1.6e-90
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 176

[Close]

BLAST of Csa5G161940 vs. TrEMBL
Match: A0A0A0KKU9_CUCSA (L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 3.6e-82
Identity = 164/176 (93.18%), Postives = 166/176 (94.32%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASP ST+N NTKISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITA SDLCIVT GARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           ESRLNLLQRNV LFQKIVP LVQFSPETILLIVSNPVDVLTY+AWKLSGF SNRVI
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVI 238

[Close]

BLAST of Csa5G161940 vs. TrEMBL
Match: A0A067KUG8_JATCU (L-lactate dehydrogenase OS=Jatropha curcas GN=JCGZ_03382 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 2.4e-78
Identity = 150/176 (85.23%), Postives = 162/176 (92.05%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S S+LGPGGLDL+QAFFKPI++ +PPS   R+TKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120
           L DEL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVT GARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           ESRLNLLQRNVALF+KI+PPLV +SPE+IL+IVSNPVDVLTYIAWKLSGFPSNRVI
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVI 176

[Close]

BLAST of Csa5G161940 vs. TrEMBL
Match: U5GU43_POPTR (L-lactate dehydrogenase OS=Populus trichocarpa GN=POPTR_0001s00440g PE=3 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 4.1e-78
Identity = 150/176 (85.23%), Postives = 161/176 (91.48%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS+S SSLGPGGLDLSQ FFKPI++ SPPS   R+TKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSASASSLGPGGLDLSQIFFKPIQNTSPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120
           L DEL LVDA PDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVT GARQIAG
Sbjct: 61  LADELALVDAIPDKLRGEMLDLQHAAAFLPRTKILASTDYSVTVGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           ESRLNLLQRNVA+FQ I+PPL Q+SP+TIL+IVSNPVDVLTY+AWKLSGFPSNRVI
Sbjct: 121 ESRLNLLQRNVAMFQGIIPPLAQYSPDTILMIVSNPVDVLTYVAWKLSGFPSNRVI 176

[Close]

BLAST of Csa5G161940 vs. TrEMBL
Match: A0A0S3T5G2_PHAAN (L-lactate dehydrogenase OS=Vigna angularis var. angularis GN=Vigan.10G205800 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.0e-76
Identity = 149/176 (84.66%), Postives = 162/176 (92.05%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           M KSSS S+LGPGGLDL+QAFFK + +A+P S   R+TKISVIGAGNVGMAIAQTILTQD
Sbjct: 3   MHKSSSGSALGPGGLDLTQAFFKSVMNAAPSSPTKRHTKISVIGAGNVGMAIAQTILTQD 62

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120
           L DELVLVDA PDKLRGEMLDLQHAAAFLPRT+I ASTDYS+TAGSDLCIVT GARQI G
Sbjct: 63  LTDELVLVDAIPDKLRGEMLDLQHAAAFLPRTRIQASTDYSVTAGSDLCIVTAGARQIHG 122

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           ESRLNLLQRNV LFQKI+PPLV++SP+TILLIVSNPVDVLTY+AWKLSGFPSNRVI
Sbjct: 123 ESRLNLLQRNVTLFQKIIPPLVRYSPDTILLIVSNPVDVLTYVAWKLSGFPSNRVI 178

[Close]

BLAST of Csa5G161940 vs. TAIR10
Match: AT4G17260.1 (AT4G17260.1 Lactate/malate dehydrogenase family protein)

HSP 1 Score: 270.8 bits (691), Expect = 6.0e-73
Identity = 132/173 (76.30%), Postives = 149/173 (86.13%), Query Frame = 1


Query: 4   SSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQDLVD 63
           +SS+  LGP GLDL+ AFFKPI ++ P   +NR TK+SV+G GNVGMAIAQTILTQDL D
Sbjct: 7   TSSLKDLGPSGLDLTSAFFKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTILTQDLAD 66

Query: 64  ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAGESR 123
           E+ LVDAKPDKLRGEMLDLQHAAAFLPRTKI+AS DY +TAGSDLCIVT GARQ  GESR
Sbjct: 67  EIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQNPGESR 126

Query: 124 LNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           LNLLQRNVALF+ I+PPL + SP++IL+IVSNPVDVLTY+AWKLSGFP NRV+
Sbjct: 127 LNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVL 179

[Close]

BLAST of Csa5G161940 vs. NCBI nr
Match: gi|778700018|ref|XP_004151663.2| (PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus])

HSP 1 Score: 340.1 bits (871), Expect = 2.3e-90
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 176

[Close]

BLAST of Csa5G161940 vs. NCBI nr
Match: gi|700195068|gb|KGN50245.1| (hypothetical protein Csa_5G161940 [Cucumis sativus])

HSP 1 Score: 340.1 bits (871), Expect = 2.3e-90
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 176

[Close]

BLAST of Csa5G161940 vs. NCBI nr
Match: gi|659073513|ref|XP_008437100.1| (PREDICTED: L-lactate dehydrogenase B [Cucumis melo])

HSP 1 Score: 328.6 bits (841), Expect = 6.9e-87
Identity = 169/176 (96.02%), Postives = 172/176 (97.73%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASPPST+NRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 83  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPPSTSNRNTKISVIGAGNVGMAIAQTILTQD 142

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS TAGS+LCIVT GARQIAG
Sbjct: 143 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAG 202

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTY+AWKLSGFPSNRVI
Sbjct: 203 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVI 258

[Close]

BLAST of Csa5G161940 vs. NCBI nr
Match: gi|778700014|ref|XP_011654796.1| (PREDICTED: L-lactate dehydrogenase B-like [Cucumis sativus])

HSP 1 Score: 312.4 bits (799), Expect = 5.1e-82
Identity = 164/176 (93.18%), Postives = 166/176 (94.32%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASP ST+N NTKISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITA SDLCIVT GARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           ESRLNLLQRNV LFQKIVP LVQFSPETILLIVSNPVDVLTY+AWKLSGF SNRVI
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVI 238

[Close]

BLAST of Csa5G161940 vs. NCBI nr
Match: gi|802581859|ref|XP_012069951.1| (PREDICTED: L-lactate dehydrogenase B [Jatropha curcas])

HSP 1 Score: 299.7 bits (766), Expect = 3.4e-78
Identity = 150/176 (85.23%), Postives = 162/176 (92.05%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S S+LGPGGLDL+QAFFKPI++ +PPS   R+TKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120
           L DEL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVT GARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 177
           ESRLNLLQRNVALF+KI+PPLV +SPE+IL+IVSNPVDVLTYIAWKLSGFPSNRVI
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVI 176

The following BLAST results are available for this feature:

BLAST of Csa5G161940 vs. Swiss-Prot


Match Name	E-value	Identity	Description
LDH_MAIZE	1.3e-61	68.33	L-lactate dehydrogenase OS=Zea mays PE=2 SV=1	[more]
LDHA_HORVU	9.4e-60	68.13	L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1	[more]
LDHB_HORVU	1.7e-56	66.47	L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1	[more]
LDHA_CHICK	1.6e-43	62.94	L-lactate dehydrogenase A chain OS=Gallus gallus GN=LDHA PE=1 SV=3	[more]
LDH_CAEEL	1.6e-43	60.28	L-lactate dehydrogenase OS=Caenorhabditis elegans GN=ldh-1 PE=2 SV=1	[more]

BLAST of Csa5G161940 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKM5_CUCSA	1.6e-90	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G161940 PE=4 SV=1	[more]
A0A0A0KKU9_CUCSA	3.6e-82	93.18	L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1	[more]
A0A067KUG8_JATCU	2.4e-78	85.23	L-lactate dehydrogenase OS=Jatropha curcas GN=JCGZ_03382 PE=3 SV=1	[more]
U5GU43_POPTR	4.1e-78	85.23	L-lactate dehydrogenase OS=Populus trichocarpa GN=POPTR_0001s00440g PE=3 SV=1	[more]
A0A0S3T5G2_PHAAN	1.0e-76	84.66	L-lactate dehydrogenase OS=Vigna angularis var. angularis GN=Vigan.10G205800 PE=...	[more]

BLAST of Csa5G161940 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G17260.1	6.0e-73	76.30	Lactate/malate dehydrogenase family protein	[more]

BLAST of Csa5G161940 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778700018\|ref\|XP_004151663.2\|	2.3e-90	100.00	PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus]	[more]
gi\|700195068\|gb\|KGN50245.1\|	2.3e-90	100.00	hypothetical protein Csa_5G161940 [Cucumis sativus]	[more]
gi\|659073513\|ref\|XP_008437100.1\|	6.9e-87	96.02	PREDICTED: L-lactate dehydrogenase B [Cucumis melo]	[more]
gi\|778700014\|ref\|XP_011654796.1\|	5.1e-82	93.18	PREDICTED: L-lactate dehydrogenase B-like [Cucumis sativus]	[more]
gi\|802581859\|ref\|XP_012069951.1\|	3.4e-78	85.23	PREDICTED: L-lactate dehydrogenase B [Jatropha curcas]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001236	Lactate/malate_DH_N
IPR001557	L-lactate/malate_DH
IPR016040	NAD(P)-bd_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process
GO:0019752	carboxylic acid metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006534	cysteine metabolic process
biological_process	GO:0006094	gluconeogenesis
biological_process	GO:0006096	glycolytic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004459	L-lactate dehydrogenase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU105296	cucumber EST collection version 3.0	transcribed_cluster
CU132509	cucumber EST collection version 3.0	transcribed_cluster
CU165523	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa5G161940.1	Csa5G161940.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU105296	CU105296	transcribed_cluster
CU165523	CU165523	transcribed_cluster
CU132509	CU132509	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001236	Lactate/malate dehydrogenase, N-terminal	PFAM	PF00056	Ldh_1_N	coord: 38..176 score: 4.7
IPR001557	L-lactate/malate dehydrogenase	PRINTS	PR00086	LLDHDRGNASE	coord: 151..171 score: 5.6E-35coord: 64..88 score: 5.6E-35coord: 39..63 score: 5.6
IPR001557	L-lactate/malate dehydrogenase	PANTHER	PTHR11540	MALATE AND LACTATE DEHYDROGENASE	coord: 10..176 score: 1.8
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 29..176 score: 5.2
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 24..176 score: 1.86
None	No IPR available	PANTHER	PTHR11540:SF3	L-LACTATE DEHYDROGENASE	coord: 10..176 score: 1.8

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa5G161940	Cucurbita maxima (Rimu)	cmacuB298
Csa5G161940	Cucurbita maxima (Rimu)	cmacuB613
Csa5G161940	Cucurbita maxima (Rimu)	cmacuB838
Csa5G161940	Cucurbita moschata (Rifu)	cmocuB286
Csa5G161940	Cucurbita moschata (Rifu)	cmocuB605
Csa5G161940	Cucurbita moschata (Rifu)	cmocuB826
Csa5G161940	Melon (DHL92) v3.5.1	cumeB357
Csa5G161940	Melon (DHL92) v3.5.1	cumeB401
Csa5G161940	Watermelon (Charleston Gray)	cuwcgB351
Csa5G161940	Watermelon (Charleston Gray)	cuwcgB364
Csa5G161940	Watermelon (97103) v1	cuwmB375
Csa5G161940	Watermelon (97103) v1	cuwmB408
Csa5G161940	Cucurbita pepo (Zucchini)	cpecuB174
Csa5G161940	Cucurbita pepo (Zucchini)	cpecuB732
Csa5G161940	Cucurbita pepo (Zucchini)	cpecuB872
Csa5G161940	Bottle gourd (USVL1VR-Ls)	culsiB322
Csa5G161940	Bottle gourd (USVL1VR-Ls)	culsiB386
Csa5G161940	Cucumber (Gy14) v2	cgybcuB224
Csa5G161940	Melon (DHL92) v3.6.1	cumedB336
Csa5G161940	Silver-seed gourd	carcuB0573
Csa5G161940	Silver-seed gourd	carcuB0938
Csa5G161940	Watermelon (97103) v2	cuwmbB336
Csa5G161940	Watermelon (97103) v2	cuwmbB356
Csa5G161940	Wax gourd	cuwgoB438
Csa5G161940	Cucumber (Chinese Long) v2	cucuB100
Csa5G161940	Cucumber (Gy14) v1	cgycuB434
Csa5G161940	Cucumber (Gy14) v1	cgycuB461