Csa5G126710 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa5G126710
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionProline-tRNA ligase; contains IPR004499 (Proline-tRNA ligase, class IIa, archaeal-type)
LocationChr5 : 3122630 .. 3123927 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGTGTGCATTTTGTTCTGTTTAAAGCATGATAGTGTAATTTAGGTTTCATTTGCCGCATACATTTTTGGATTTTATATATGATTGGCTATTTTTTAAGAGTCAAAACTGGATTTCTTTTCTTTTTTAAGTTTGGAGATTGTTAATGAATTCTTTTAGAGGTTTTTTTTCTTTCTTTTTTTAATTTTTATCTTTTTATTTAAGTCATGTGCTTTATATTTTTGCCATGTGTTAGGTCTAATTAAATTTTTTTGCATCATGAACCATGTTCAAATGTTCATAGATGAACCAAGCGGTGAAGCTTTTATGCTTCTGTTTTCTCCTTTTTCCTTTTTGGTAGAAAACACAGTTATATAAACAATTGGAAGAATATAAAGGGGAGAGGGAAGGTTAAATCTCCATCAAATTCTTAACTAAGCTACGGGAGATTACCAAAATACCCTTCAATCTAAGTCTGAAATTTTGCAGTTCTTTTGTGTAACTGTGATCATTATCTAAAGGTCATTTTTATTTCAGGTTCTTGAGATATTGGAACTGTATAGACGCATATATGAAGAATACCTGGTGATTCCTGTTATTAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGTATAAGTTGACTAAATTGCTATGACCCATCTTCAGCATGGCAAATTGATATCCATCACTTGTGTAAGTGGTATAAAACCATTGTTCCGTTTGTGATATGCAGGCATTTATCCCAAACACTGGTCGTGGTATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAATTGTTTGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACCAGAACGGTAATATTTTGATACTTGCATTTGTCGTAGCCTTTGGTAACTTGTTAGAGTGAATTTCTGCGTCTTGTTAATCTAGCCTTCCAAACTGTTTGTAGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCTCCAAGTTGCATCAATTCAAGTCATTATAGTTCCTGTTCCCTACAAAGATGCAGACACTCAAGGAATTTTTTATGCTTGTTCTGCCACTTCGGATATGTTGTCCAAAGCAGGAATTCGTGCAGAGGTAGACATTGGGGAGAACTATTCTCCTGGATGGAAATACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCCAAGGACTTAGCAAACAATCAGGTTAGATATTTTGTCTATTAA

mRNA sequence

ATGAGGTCATTTTTATTTCAGGTTCTTGAGATATTGGAACTGTATAGACGCATATATGAAGAATACCTGGTGATTCCTGTTATTAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGCATTTATCCCAAACACTGGTCGTGGTATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAATTGTTTGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACCAGAACGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCTCCAAGTTGCATCAATTCAAGTCATTATAGTTCCTGTTCCCTACAAAGATGCAGACACTCAAGGAATTTTTTATGCTTGTTCTGCCACTTCGGATATGTTGTCCAAAGCAGGAATTCGTGCAGAGGTAGACATTGGGGAGAACTATTCTCCTGGATGGAAATACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCCAAGGACTTAGCAAACAATCAGGTTAGATATTTTGTCTATTAA

Coding sequence (CDS)

ATGAGGTCATTTTTATTTCAGGTTCTTGAGATATTGGAACTGTATAGACGCATATATGAAGAATACCTGGTGATTCCTGTTATTAAGGGCAAGAAGAGTGAGATGGAGAAGTTTGCTGGTGGTCTTTACACCACAAGCGTTGAGGCATTTATCCCAAACACTGGTCGTGGTATTCAAGGTGCGACTTCACATTGTTTGGGTCAAAATTTTGCAAAATTGTTTGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACCAGAACGATTGGTGTGATGGTTATGGTTCACGGTGATGACAAGGGATTGGTGATGCCTCTCCAAGTTGCATCAATTCAAGTCATTATAGTTCCTGTTCCCTACAAAGATGCAGACACTCAAGGAATTTTTTATGCTTGTTCTGCCACTTCGGATATGTTGTCCAAAGCAGGAATTCGTGCAGAGGTAGACATTGGGGAGAACTATTCTCCTGGATGGAAATACTCCCACTGGGAGATGAAAGGTGTTCCACTCAGGATTGAAATAGGGCCCAAGGACTTAGCAAACAATCAGGTTAGATATTTTGTCTATTAA

Protein sequence

MRSFLFQVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRYFVY*
Homology
[Close]
BLAST of Csa5G126710 vs. Swiss-Prot
Match: SYPC_ARATH (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana GN=At3g62120 PE=2 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 4.9e-89
Identity = 152/190 (80.00%), Postives = 172/190 (90.53%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +VL+ILELYRRIYEEYL +PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCL
Sbjct: 218 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 277

Query: 67  GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 126
           GQNFAK+FEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLV+P +VAS+QV++
Sbjct: 278 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 337

Query: 127 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 186
           +PVPYKDA+TQGI+ AC+AT+  L +AGIRAE D+ +NYSPGWKYS WEMKGVPLRIEIG
Sbjct: 338 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 397

Query: 187 PKDLANNQVR 197
           P+DL N+QVR
Sbjct: 398 PRDLENDQVR 407

[Close]
BLAST of Csa5G126710 vs. Swiss-Prot
Match: SYEP_MOUSE (Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus GN=Eprs PE=1 SV=4)

HSP 1 Score: 225.3 bits (573), Expect = 5.9e-58
Identity = 109/194 (56.19%), Postives = 144/194 (74.23%), Query Frame = 1 

Query: 7    QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
            +VL+ILELY R+YEE L IPV++G+K+E EKFAGG YTT++EAFI  +GR IQGATSH L
Sbjct: 1185 EVLQILELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTIEAFISASGRAIQGATSHHL 1244

Query: 67   GQNFAKLFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQV 126
            GQNF+K+ EI FE+ K  GEK   +Q SW  +TRTIGVMVMVHGD+ GLV+P +VAS+QV
Sbjct: 1245 GQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIGVMVMVHGDNMGLVLPPRVASVQV 1304

Query: 127  IIVPVPYKDA----DTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVP 186
            +++P    +A    D + +   C+     L  A IR  VD+ +NYSPGWK++HWE+KGVP
Sbjct: 1305 VVIPCGITNALSEEDREALMAKCNEYRRRLLGANIRVRVDLRDNYSPGWKFNHWELKGVP 1364

Query: 187  LRIEIGPKDLANNQ 195
            +R+E+GP+D+ + Q
Sbjct: 1365 VRLEVGPRDMKSCQ 1378

[Close]
BLAST of Csa5G126710 vs. Swiss-Prot
Match: SYEP_HUMAN (Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens GN=EPRS PE=1 SV=5)

HSP 1 Score: 223.4 bits (568), Expect = 2.2e-57
Identity = 106/194 (54.64%), Postives = 141/194 (72.68%), Query Frame = 1 

Query: 7    QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
            +VL+IL+LY ++YEE L IPV+KG+K+E EKFAGG YTT++EAFI  +GR IQG TSH L
Sbjct: 1185 EVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHL 1244

Query: 67   GQNFAKLFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQV 126
            GQNF+K+FEI FE+ K  GEK   +QNSW  +TRTIGVM MVHGD+ GLV+P +VA +QV
Sbjct: 1245 GQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQV 1304

Query: 127  IIVPVPYKDA----DTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVP 186
            +I+P    +A    D + +   C+     L    IR   D+ +NYSPGWK++HWE+KGVP
Sbjct: 1305 VIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVP 1364

Query: 187  LRIEIGPKDLANNQ 195
            +R+E+GP+D+ + Q
Sbjct: 1365 IRLEVGPRDMKSCQ 1378

[Close]
BLAST of Csa5G126710 vs. Swiss-Prot
Match: SYEP_DROME (Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster GN=Aats-glupro PE=1 SV=2)

HSP 1 Score: 223.0 bits (567), Expect = 2.9e-57
Identity = 111/194 (57.22%), Postives = 140/194 (72.16%), Query Frame = 1 

Query: 7    QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
            +VL+IL+LY  +Y   L IPV+KG+K+E EKFAGG YTT+VEAFI  +GR IQGATSH L
Sbjct: 1386 EVLDILDLYALVYTHLLAIPVVKGRKTEKEKFAGGDYTTTVEAFISASGRAIQGATSHHL 1445

Query: 67   GQNFAKLFEINFEN-EKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVI 126
            GQNF+K+FEI +E+ E  +K  V+QNSW  +TRTIGVM+MVH D++GLV+P  VA IQ I
Sbjct: 1446 GQNFSKMFEIVYEDPETQQKKYVYQNSWGITTRTIGVMIMVHADNQGLVLPPHVACIQAI 1505

Query: 127  IVP----VPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPL 186
            +VP    V  KD +   +  AC A    L   G+R E D  +NYSPGWK++HWE+KGVPL
Sbjct: 1506 VVPCGITVNTKDDERAQLLDACKALEKRLVGGGVRCEGDYRDNYSPGWKFNHWELKGVPL 1565

Query: 187  RIEIGPKDLANNQV 196
            R+E+GPKDL   Q+
Sbjct: 1566 RLEVGPKDLKAQQL 1579

[Close]
BLAST of Csa5G126710 vs. Swiss-Prot
Match: PRS1_SCHPO (Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=prs1 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.9e-56
Identity = 113/201 (56.22%), Postives = 136/201 (67.66%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +V +IL+LY RIY + L +PVIKG KSE EKFAGG++TT+VE +IP TGRGIQGATSHCL
Sbjct: 376 EVHQILDLYARIYTDLLAVPVIKGVKSENEKFAGGMFTTTVEGYIPTTGRGIQGATSHCL 435

Query: 67  GQNFAKLFEINFENEKGE--------KAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQ 126
           GQNF+K+F I  E+   E        K  VWQNSW  STRTIGV VMVHGDDKGL +P  
Sbjct: 436 GQNFSKMFNIVVEDPNAEIGPTGERPKLFVWQNSWGLSTRTIGVAVMVHGDDKGLKLPPA 495

Query: 127 VASIQVIIVPV----PYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHW 186
           +A +Q ++VP        D +   I   CS  +D L+ A IR E D+   Y+PG+K+SHW
Sbjct: 496 IALVQSVVVPCGITNKTTDQERNEIEGFCSKLADRLNAADIRTEADL-RAYTPGYKFSHW 555

Query: 187 EMKGVPLRIEIGPKDLANNQV 196
           EMKGVPLR+E GP D   NQV
Sbjct: 556 EMKGVPLRLEYGPNDAKKNQV 575

[Close]
BLAST of Csa5G126710 vs. TrEMBL
Match: A0A0A0KMY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G126710 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 4.2e-111
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 1 

Query: 1   MRSFLFQVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQG 60
           MRSFLFQVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQG
Sbjct: 1   MRSFLFQVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQG 60

Query: 61  ATSHCLGQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVA 120
           ATSHCLGQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVA
Sbjct: 61  ATSHCLGQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVA 120

Query: 121 SIQVIIVPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVP 180
           SIQVIIVPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVP
Sbjct: 121 SIQVIIVPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVP 180

Query: 181 LRIEIGPKDLANNQVRYFVY 201
           LRIEIGPKDLANNQVRYFVY
Sbjct: 181 LRIEIGPKDLANNQVRYFVY 200

[Close]
BLAST of Csa5G126710 vs. TrEMBL
Match: A0A0A0KTZ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G015750 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 1.7e-96
Identity = 175/190 (92.11%), Postives = 182/190 (95.79%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYL IPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL
Sbjct: 199 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 258

Query: 67  GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 126
           GQNFAK+FEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLV+P +VAS+QVII
Sbjct: 259 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVII 318

Query: 127 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADTQGIF ACSAT D L+ AGIRAEVD  +NYSPGWKYSHWEMKGVPLRIEIG
Sbjct: 319 VPVPYKDADTQGIFDACSATLDTLTAAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIG 378

Query: 187 PKDLANNQVR 197
           PKDLANNQVR
Sbjct: 379 PKDLANNQVR 388

[Close]
BLAST of Csa5G126710 vs. TrEMBL
Match: A0A067JHA4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00194 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 1.6e-94
Identity = 168/190 (88.42%), Postives = 181/190 (95.26%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +VL+ILELYRRIYEE+L IPVIKG+KSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL
Sbjct: 187 EVLQILELYRRIYEEFLAIPVIKGRKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 246

Query: 67  GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 126
           GQNFAK+FEINFENEKGEK MVWQNSWAYSTRTIGVMVMVHGDDKGLV+P ++AS+QVI+
Sbjct: 247 GQNFAKMFEINFENEKGEKGMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPRIASVQVIV 306

Query: 127 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 186
           VPVPYKDA+TQGIF AC+AT D LSKAGIRAE D  +NYSPGWKYSHWEMKGVPLRIEIG
Sbjct: 307 VPVPYKDANTQGIFDACAATVDALSKAGIRAEADFRDNYSPGWKYSHWEMKGVPLRIEIG 366

Query: 187 PKDLANNQVR 197
           PKDLANNQVR
Sbjct: 367 PKDLANNQVR 376

[Close]
BLAST of Csa5G126710 vs. TrEMBL
Match: B9GTA1_POPTR (tRNA synthetase-related family protein OS=Populus trichocarpa GN=POPTR_0002s18810g PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 2.1e-94
Identity = 169/190 (88.95%), Postives = 179/190 (94.21%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +VL+ILELYRRIYEE+L IPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL
Sbjct: 186 EVLQILELYRRIYEEFLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 245

Query: 67  GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 126
           GQNFAK+FEINFENEKGEK M WQNSWAYSTRTIGVMVMVHGDDKGLV+P +VAS+QVI+
Sbjct: 246 GQNFAKMFEINFENEKGEKGMAWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIV 305

Query: 127 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADTQGIF AC+AT D L +AGIRAE D  ENYSPGWKYSHWEMKGVPLRIEIG
Sbjct: 306 VPVPYKDADTQGIFDACAATVDTLCEAGIRAEADFRENYSPGWKYSHWEMKGVPLRIEIG 365

Query: 187 PKDLANNQVR 197
           PKDLANNQVR
Sbjct: 366 PKDLANNQVR 375

[Close]
BLAST of Csa5G126710 vs. TrEMBL
Match: A0A0L9U988_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan03g272500 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 4.6e-94
Identity = 168/190 (88.42%), Postives = 180/190 (94.74%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +VL+ILELYRRIYEEYL +PVIKGKKSE+EKFAGGLYTTSVEAFIPNTGRGIQGATSHCL
Sbjct: 235 EVLDILELYRRIYEEYLAVPVIKGKKSELEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 294

Query: 67  GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 126
           GQNFAK+FEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLV+P +VAS+QVI+
Sbjct: 295 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIV 354

Query: 127 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 186
           +PVPYKDADTQGIF ACSAT + LS AGIRAE D  +NYSPGWKYSHWEMKGVPLRIEIG
Sbjct: 355 IPVPYKDADTQGIFDACSATVNALSDAGIRAESDFRDNYSPGWKYSHWEMKGVPLRIEIG 414

Query: 187 PKDLANNQVR 197
           PKDLAN QVR
Sbjct: 415 PKDLANKQVR 424

[Close]
BLAST of Csa5G126710 vs. TAIR10
Match: AT3G62120.1 (AT3G62120.1 Class II aaRS and biotin synthetases superfamily protein)

HSP 1 Score: 328.6 bits (841), Expect = 2.8e-90
Identity = 152/190 (80.00%), Postives = 172/190 (90.53%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +VL+ILELYRRIYEEYL +PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCL
Sbjct: 218 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 277

Query: 67  GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 126
           GQNFAK+FEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLV+P +VAS+QV++
Sbjct: 278 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 337

Query: 127 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 186
           +PVPYKDA+TQGI+ AC+AT+  L +AGIRAE D+ +NYSPGWKYS WEMKGVPLRIEIG
Sbjct: 338 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 397

Query: 187 PKDLANNQVR 197
           P+DL N+QVR
Sbjct: 398 PRDLENDQVR 407

[Close]
BLAST of Csa5G126710 vs. TAIR10
Match: AT5G52520.1 (AT5G52520.1 Class II aaRS and biotin synthetases superfamily protein)

HSP 1 Score: 188.7 bits (478), Expect = 3.4e-48
Identity = 87/186 (46.77%), Postives = 127/186 (68.28%), Query Frame = 1 

Query: 10  EILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQN 69
           +++E+Y R   E   IPVI G+KS++E FAG   T ++EA + +  + +Q  TSH LGQN
Sbjct: 236 QMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEAMMGDR-KALQAGTSHNLGQN 295

Query: 70  FAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVIIVPV 129
           F++ F   F +E GE+  VWQ SWA STR +G ++M HGDD GL++P ++A IQV+IVP+
Sbjct: 296 FSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGDDTGLMLPPKIAPIQVVIVPI 355

Query: 130 PYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIGPKD 189
             KD +  G+  A S+  + L  AG+R ++D  +  +PGWK++ WEMKG+PLRIEIGP+D
Sbjct: 356 WKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGWKFNFWEMKGIPLRIEIGPRD 415

Query: 190 LANNQV 196
           +++N V
Sbjct: 416 VSSNSV 420

[Close]
BLAST of Csa5G126710 vs. TAIR10
Match: AT5G10880.1 (AT5G10880.1 tRNA synthetase-related / tRNA ligase-related)

HSP 1 Score: 110.9 bits (276), Expect = 9.1e-25
Identity = 56/93 (60.22%), Postives = 65/93 (69.89%), Query Frame = 1 

Query: 105 MVHGDDKGLVMPLQVASIQVIIVPVPYKDA-DTQGIFYACSATSDMLSKAGIRAEVDIGE 164
           M HGDDKGLV P +VA +QV+++ VP K A D Q +  AC A    L  AGIRAE DI +
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 165 NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVR 197
           NYS GWKY+  E+ GVPLRIE GP+DLAN+QVR
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVR 181

[Close]
BLAST of Csa5G126710 vs. NCBI nr
Match: gi|700194605|gb|KGN49782.1| (hypothetical protein Csa_5G126710 [Cucumis sativus])

HSP 1 Score: 408.7 bits (1049), Expect = 6.0e-111
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 1 

Query: 1   MRSFLFQVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQG 60
           MRSFLFQVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQG
Sbjct: 1   MRSFLFQVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQG 60

Query: 61  ATSHCLGQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVA 120
           ATSHCLGQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVA
Sbjct: 61  ATSHCLGQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVA 120

Query: 121 SIQVIIVPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVP 180
           SIQVIIVPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVP
Sbjct: 121 SIQVIIVPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVP 180

Query: 181 LRIEIGPKDLANNQVRYFVY 201
           LRIEIGPKDLANNQVRYFVY
Sbjct: 181 LRIEIGPKDLANNQVRYFVY 200

[Close]
BLAST of Csa5G126710 vs. NCBI nr
Match: gi|778708193|ref|XP_011656143.1| (PREDICTED: putative proline--tRNA ligase C19C7.06 [Cucumis sativus])

HSP 1 Score: 386.7 bits (992), Expect = 2.4e-104
Identity = 189/190 (99.47%), Postives = 190/190 (100.00%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL
Sbjct: 49  EVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 108

Query: 67  GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 126
           GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII
Sbjct: 109 GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 168

Query: 127 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG
Sbjct: 169 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 228

Query: 187 PKDLANNQVR 197
           PKDLANNQVR
Sbjct: 229 PKDLANNQVR 238

[Close]
BLAST of Csa5G126710 vs. NCBI nr
Match: gi|659072589|ref|XP_008466319.1| (PREDICTED: putative proline--tRNA ligase C19C7.06 isoform X2 [Cucumis melo])

HSP 1 Score: 369.0 bits (946), Expect = 5.2e-99
Identity = 180/190 (94.74%), Postives = 185/190 (97.37%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYL IPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL
Sbjct: 199 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 258

Query: 67  GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 126
           GQNFAK+FEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMP +VAS+QVII
Sbjct: 259 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 318

Query: 127 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIF ACSATSD LSKAGIRAEVDI ENYSPGWKYSHWEMKGVPLRIEIG
Sbjct: 319 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 378

Query: 187 PKDLANNQVR 197
           PKDLANNQVR
Sbjct: 379 PKDLANNQVR 388

[Close]
BLAST of Csa5G126710 vs. NCBI nr
Match: gi|659072593|ref|XP_008466335.1| (PREDICTED: putative proline--tRNA ligase C19C7.06 isoform X3 [Cucumis melo])

HSP 1 Score: 369.0 bits (946), Expect = 5.2e-99
Identity = 180/190 (94.74%), Postives = 185/190 (97.37%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYL IPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL
Sbjct: 199 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 258

Query: 67  GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 126
           GQNFAK+FEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMP +VAS+QVII
Sbjct: 259 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 318

Query: 127 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIF ACSATSD LSKAGIRAEVDI ENYSPGWKYSHWEMKGVPLRIEIG
Sbjct: 319 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 378

Query: 187 PKDLANNQVR 197
           PKDLANNQVR
Sbjct: 379 PKDLANNQVR 388

[Close]
BLAST of Csa5G126710 vs. NCBI nr
Match: gi|659072595|ref|XP_008466343.1| (PREDICTED: putative proline--tRNA ligase C19C7.06 isoform X4 [Cucumis melo])

HSP 1 Score: 369.0 bits (946), Expect = 5.2e-99
Identity = 180/190 (94.74%), Postives = 185/190 (97.37%), Query Frame = 1 

Query: 7   QVLEILELYRRIYEEYLVIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 66
           +VLEILELYRRIYEEYL IPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL
Sbjct: 149 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 208

Query: 67  GQNFAKLFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPLQVASIQVII 126
           GQNFAK+FEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMP +VAS+QVII
Sbjct: 209 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVMPPKVASVQVII 268

Query: 127 VPVPYKDADTQGIFYACSATSDMLSKAGIRAEVDIGENYSPGWKYSHWEMKGVPLRIEIG 186
           VPVPYKDADT+GIF ACSATSD LSKAGIRAEVDI ENYSPGWKYSHWEMKGVPLRIEIG
Sbjct: 269 VPVPYKDADTRGIFDACSATSDALSKAGIRAEVDIRENYSPGWKYSHWEMKGVPLRIEIG 328

Query: 187 PKDLANNQVR 197
           PKDLANNQVR
Sbjct: 329 PKDLANNQVR 338

The following BLAST results are available for this feature:
BLAST of Csa5G126710 vs. Swiss-Prot
Match NameE-valueIdentityDescription
SYPC_ARATH4.9e-8980.00Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana GN=At3g62120 PE=2 SV=1[more]
SYEP_MOUSE5.9e-5856.19Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus GN=Eprs PE=1 SV=4[more]
SYEP_HUMAN2.2e-5754.64Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens GN=EPRS PE=1 SV=5[more]
SYEP_DROME2.9e-5757.22Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster GN=Aats-g... [more]
PRS1_SCHPO1.9e-5656.22Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 ... [more]
BLAST of Csa5G126710 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KMY8_CUCSA4.2e-111100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G126710 PE=4 SV=1[more]
A0A0A0KTZ1_CUCSA1.7e-9692.11Uncharacterized protein OS=Cucumis sativus GN=Csa_4G015750 PE=3 SV=1[more]
A0A067JHA4_JATCU1.6e-9488.42Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00194 PE=3 SV=1[more]
B9GTA1_POPTR2.1e-9488.95tRNA synthetase-related family protein OS=Populus trichocarpa GN=POPTR_0002s1881... [more]
A0A0L9U988_PHAAN4.6e-9488.42Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan03g272500 PE=3 SV=1[more]
BLAST of Csa5G126710 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G62120.12.8e-9080.00 Class II aaRS and biotin synthetases superfamily protein[more]
AT5G52520.13.4e-4846.77 Class II aaRS and biotin synthetases superfamily protein[more]
AT5G10880.19.1e-2560.22 tRNA synthetase-related / tRNA ligase-related[more]
BLAST of Csa5G126710 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700194605|gb|KGN49782.1|6.0e-111100.00hypothetical protein Csa_5G126710 [Cucumis sativus][more]
gi|778708193|ref|XP_011656143.1|2.4e-10499.47PREDICTED: putative proline--tRNA ligase C19C7.06 [Cucumis sativus][more]
gi|659072589|ref|XP_008466319.1|5.2e-9994.74PREDICTED: putative proline--tRNA ligase C19C7.06 isoform X2 [Cucumis melo][more]
gi|659072593|ref|XP_008466335.1|5.2e-9994.74PREDICTED: putative proline--tRNA ligase C19C7.06 isoform X3 [Cucumis melo][more]
gi|659072595|ref|XP_008466343.1|5.2e-9994.74PREDICTED: putative proline--tRNA ligase C19C7.06 isoform X4 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004154Anticodon-bd
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006525 arginine metabolic process
biological_process GO:0006560 proline metabolic process
biological_process GO:0006433 prolyl-tRNA aminoacylation
biological_process GO:0015074 DNA integration
biological_process GO:0090304 nucleic acid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004827 proline-tRNA ligase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0003676 nucleic acid binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa5G126710.1Csa5G126710.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004154Anticodon-bindingGENE3DG3DSA:3.40.50.800coord: 118..197
score: 3.0
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 123..196
score: 3.8
IPR004154Anticodon-bindingunknownSSF52954Class II aaRS ABD-relatedcoord: 111..198
score: 3.78
NoneNo IPR availableGENE3DG3DSA:3.30.930.10coord: 7..116
score: 7.2
NoneNo IPR availablePANTHERPTHR11451TRNA SYNTHETASE-RELATEDcoord: 7..197
score: 3.1E
NoneNo IPR availablePANTHERPTHR11451:SF21BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASEcoord: 7..197
score: 3.1E
NoneNo IPR availableunknownSSF55681Class II aaRS and biotin synthetasescoord: 8..110
score: 9.32