Csa4G658540 (gene) Cucumber (Chinese Long) v2

BLAST of Csa4G658540 vs. Swiss-Prot
Match: AAE1_ARATH (Probable acyl-activating enzyme 1, peroxisomal OS=Arabidopsis thaliana GN=AAE1 PE=2 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 2.6e-212
Identity = 352/552 (63.77%), Postives = 442/552 (80.07%), Query Frame = 1

Query: 1   MEG-IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60
           MEG I+  AN VPLTPI+FL+R+A V+ DR+S+VYG+V++TWR+T  RC + ASAL  +G
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120
           IS GDVV+VLAPNVPA  ELHF VPMAGA+LCTLN+RHD+++V+ LL HS  K+I ADHQ
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           +L I + A EI+S     +++P +V++ E    + +++    + + +EYE ++  G  DF
Sbjct: 123 FLQIAEGACEILSNK--GDKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDF 182

Query: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240
           E+ RP DE D IS+NYTSGTTS PKGV+YSHRG YLN+L+ VLLN+M S   YLWT PMF
Sbjct: 183 EVIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMF 242

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGW + WGV A GGTNIC RNV AK IF NIS H VTHMGGAPT+LNMI+NAP +EQK
Sbjct: 243 HCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQK 302

Query: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360
           PL GKV+ +TG APPP+++++KM ELGF + H YGLTETYG  T C WKPEWDSLP E+Q
Sbjct: 303 PLPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQ 362

Query: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420
           AK+K+RQG+ H+G+E + +K+P+TM ++PADG TMGEV+ RGNTVM GYLK+ +AT+EAF
Sbjct: 363 AKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAF 422

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
            GGWF SGDLGV+HPDGYIELKDRSKDIIISGGENIS+IEVES LF HP VLEAA+V RP
Sbjct: 423 KGGWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARP 482

Query: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           D++WGET CAFV LK GS A+ EE+I +CRD LPHYMAP+++VF+ DLPK+STGK+QK +
Sbjct: 483 DEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFE-DLPKTSTGKVQKFV 542

Query: 541 LKEEAKAMGTLS 552
           L+ +AKA+ +LS
Sbjct: 543 LRTKAKALVSLS 549

BLAST of Csa4G658540 vs. Swiss-Prot
Match: AAE2_ARATH (Probable acyl-activating enzyme 2 OS=Arabidopsis thaliana GN=AAE2 PE=2 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 8.3e-203
Identity = 344/553 (62.21%), Postives = 425/553 (76.85%), Query Frame = 1

Query: 4   IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRG 63
           +R  AN  PL+PITFLER+A V+ DR SLV+G+V+ TW +T QRC + ASAL ++GISRG
Sbjct: 52  LRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLGISRG 111

Query: 64  DVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHI 123
           DVVA LAPNVPA +ELHFAVPMAG ILC LN R D + +S LL HSEAKI+  DHQ L I
Sbjct: 112 DVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEI 171

Query: 124 VKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSA-----SDDLEYESLLNTGTLD 183
              A+++++K+    +  ++V++ + +    +     S      S D EYE+LL +G  +
Sbjct: 172 AHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSE 231

Query: 184 FEIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPM 243
           FEI +P  E DPIS+NYTSGTTSRPKGV+YSHRG YLN+L+TV L+ MS   VYLWTVPM
Sbjct: 232 FEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLWTVPM 291

Query: 244 FHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQ 303
           FHCNGW + WGVAAQGGTNIC R V+ K IF NI++H VTHMGGAPTVLNMIVN  +TE 
Sbjct: 292 FHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEH 351

Query: 304 KPLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEK 363
           KPL  +V +MTGG+PP   IL KM ELGF + H YGLTETYG  T C WKPEWDSL  E+
Sbjct: 352 KPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEE 411

Query: 364 QAKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEA 423
           + KLK+RQG+QH+G+EG+D+K+P+TME+VP DG TMGEVM RGNTVM GY KD++AT +A
Sbjct: 412 RTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKA 471

Query: 424 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGR 483
           F G WF SGDL V++PDGYIE+KDR KD+IISGGENIS++EVE VL +H +VLEAA+V R
Sbjct: 472 FEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVAR 531

Query: 484 PDDHWGETPCAFVNLKHGSNATE-EEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQK 543
           PD HWG+TPC FV LK G +  + EEII FCRD+LPHYMAPKT+VF  D+PK+STGK+QK
Sbjct: 532 PDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVF-GDIPKTSTGKVQK 591

Query: 544 LILKEEAKAMGTL 551
            +L+++A  MG+L
Sbjct: 592 YLLRKKADEMGSL 603

BLAST of Csa4G658540 vs. Swiss-Prot
Match: AAE11_ARATH (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana GN=AAE11 PE=1 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 9.0e-165
Identity = 277/551 (50.27%), Postives = 388/551 (70.42%), Query Frame = 1

Query: 4   IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRG 63
           + C AN+VPLTPITFL+RA+  + +R S++YG  RFTW +T  RC + A++L+ + I+R 
Sbjct: 5   VLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLNITRN 64

Query: 64  DVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHI 123
           DVV++LAPNVPA YE+HF+VPM GA+L  +N R DA  ++ +L H+E KI+  D+++  +
Sbjct: 65  DVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYEFAPL 124

Query: 124 VKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTG----TLDF 183
           ++  + ++  T  ++  PRI+++ E D  +         S +L+YE L+  G    +   
Sbjct: 125 IQEVLRLIP-TYQSQPHPRIILINEIDSTTKPF------SKELDYEGLIRKGEPTPSSSA 184

Query: 184 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 243
            + R  +E DPISLNYTSGTT+ PKGV+ SH+G YL+ LS+++  +M    VYLWT+PMF
Sbjct: 185 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMF 244

Query: 244 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 303
           HCNGW+ TW VAA+GGTN+C R+V A EI+ NI LH VTHM   PTV   ++    T+Q 
Sbjct: 245 HCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQS 304

Query: 304 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 363
           P    V V+TGG+ PP+ ++ K+ +LGF ++H YGLTE  G   FC W+ EW+ LP+ +Q
Sbjct: 305 PKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQ 364

Query: 364 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 423
            +L+ RQG++++ +  VD+KN  T+ESVP DGKTMGE++I+G+++M GYLK+ KAT EAF
Sbjct: 365 IELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAF 424

Query: 424 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 483
             GW  +GD+GV HPDGY+E+KDRSKDIIISGGENIS+IEVE VL+ +  VLEAA+V  P
Sbjct: 425 KHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMP 484

Query: 484 DDHWGETPCAFVNLKHGSN---ATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQ 543
              WGETPCAFV LK G      +E ++IK+CR+N+PH+M PK VVF  +LPK+S GKI 
Sbjct: 485 HPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKIL 544

Query: 544 KLILKEEAKAM 548
           K  L++ AKA+
Sbjct: 545 KSKLRDIAKAL 548

BLAST of Csa4G658540 vs. Swiss-Prot
Match: AAE20_ARATH (Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana GN=AAE20 PE=1 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 2.7e-161
Identity = 270/557 (48.47%), Postives = 384/557 (68.94%), Query Frame = 1

Query: 6   CSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRGDV 65
           C AN+VPLTP+TFL+RA+  + +R S++YG  RFTW +T  RC + A++L+ + IS+ DV
Sbjct: 7   CEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNISKNDV 66

Query: 66  VAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHIVK 125
           V+V+APN PA YE+HFAVPMAGA+L  +N R DA  ++ +L H++ KI+  D  +  + +
Sbjct: 67  VSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRSFEALAR 126

Query: 126 AAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTG----TLDFEI 185
            ++ ++S   +   LP ++ + E D P        ++ ++L+YE L+  G    ++   +
Sbjct: 127 ESLHLLSSEDSNLNLP-VIFIHENDFPKR------ASFEELDYECLIQRGEPTPSMVARM 186

Query: 186 RRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFHC 245
            R  DE DPISLNYTSGTT+ PKGV+ SHRG YL TLS ++  +M +  VYLWT+PMFHC
Sbjct: 187 FRIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHC 246

Query: 246 NGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKPL 305
           NGW+ TWG AA+GGT++C R+V A EI+ NI +HNVTHM   PTV N+++     +  P 
Sbjct: 247 NGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPR 306

Query: 306 LGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQAK 365
            G V V+TGG+PPP+ ++ K++ LGF ++H YG TE  G   FC W+ EW+ LP+ +Q +
Sbjct: 307 SGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQME 366

Query: 366 LKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFNG 425
           LK+RQG+  +G+  VD+KN  T +S P DGKTMGE++I+G+++M GYLK+ KAT EAF  
Sbjct: 367 LKARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKH 426

Query: 426 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPDD 485
           GW  +GD+GV HPDG++E+KDRSKDIIISGGENIS++EVE+VL+ +P VLE A+V  P  
Sbjct: 427 GWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHP 486

Query: 486 HWGETPCAFVNLKHGSNATEEE-----------IIKFCRDNLPHYMAPKTVVFKSDLPKS 545
            WGETPCAFV L+      +E+           +I++CR+NLPH+M P+ VVF  +LPK+
Sbjct: 487 TWGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKN 546

Query: 546 STGKIQKLILKEEAKAM 548
             GKI K  L++ AK +
Sbjct: 547 GNGKILKPKLRDIAKGL 556

BLAST of Csa4G658540 vs. Swiss-Prot
Match: AAE12_ARATH (Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana GN=AAE12 PE=2 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 2.3e-160
Identity = 269/556 (48.38%), Postives = 382/556 (68.71%), Query Frame = 1

Query: 6   CSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRGDV 65
           C AN+VPLTPITFL+RA+  + +R S++YG  RFTW +T  RC + A++L+ + I + DV
Sbjct: 7   CEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNIGKNDV 66

Query: 66  VAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHIVK 125
           V+V+APN PA YE+HFAVPMAGA+L  +N R DA  ++ +L H++ KI+     +  + +
Sbjct: 67  VSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRSFEPLAR 126

Query: 126 AAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGT-LDFEIRRP 185
             ++++S   +   LP ++ + E D P         +S++ +YE L+  G      + R 
Sbjct: 127 EILQLLSSEDSNLNLP-VIFIHEIDFPKR------VSSEESDYECLIQRGEPTPLLLARM 186

Query: 186 I---DERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFHC 245
               DE DPISLNYTSGTT+ PKGV+ SHRG YL+TLS ++  +M +  VYLWT+PMFHC
Sbjct: 187 FCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHC 246

Query: 246 NGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKPL 305
           NGW+ TWG AA+GGT++C R+V A EI+ NI +HNVTHM   PTV N+++     +    
Sbjct: 247 NGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHR 306

Query: 306 LGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQAK 365
            G V V+TGG+PPP+ ++ K++ LGF ++H YGLTE  G   FC W+ EW+ LP+ +Q +
Sbjct: 307 SGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQME 366

Query: 366 LKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFNG 425
           LK+RQG+  +G+  VD++N  T ESVP DGKTMGE++++G+++M GYLK+ KAT EAF  
Sbjct: 367 LKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKH 426

Query: 426 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPDD 485
           GW  SGD+GV HPDG++E+KDRSKDIIISGGENIS++EVE++++ +P VLE A+V  P  
Sbjct: 427 GWLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHP 486

Query: 486 HWGETPCAFVNLKHGSNATEE----------EIIKFCRDNLPHYMAPKTVVFKSDLPKSS 545
            WGETPCAFV L+ G    E+          ++I++CR+NLPH+M P+ VVF  +LPK+ 
Sbjct: 487 TWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNG 546

Query: 546 TGKIQKLILKEEAKAM 548
            GKI K  L++ AK +
Sbjct: 547 NGKILKPKLRDIAKGL 555

BLAST of Csa4G658540 vs. TrEMBL
Match: A0A0A0L1B6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G658530 PE=4 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 1.1e-251
Identity = 425/553 (76.85%), Postives = 485/553 (87.70%), Query Frame = 1

Query: 5   RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRGD 64
           RCSAN VPLTPI+FLER+AAV+GDRISLVYG V++TWR+TLQRCT+ ASALV  GI+RGD
Sbjct: 6   RCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTGIARGD 65

Query: 65  VVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHIV 124
           VVA L PN+PA YELHFAVPMAGA+LC+LN RHDAAMVSTLL HSEAKIIV D+Q  HIV
Sbjct: 66  VVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQLEHIV 125

Query: 125 KAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDFEIRRP 184
             AI+ MS+    E+LPR+VI+QE+D P S I+   SA + LE+ES L +G L+FEIRRP
Sbjct: 126 TGAIKAMSERK--EKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEIRRP 185

Query: 185 IDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFHCNGW 244
            DE DPI+LNYTSGTTSRPKGVI+SHRG YLN+LS VLLNDM S+ VYLWTVPMFHCNGW
Sbjct: 186 RDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGW 245

Query: 245 SMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKPLLGK 304
            +TWGVAAQ GTNICQRNV AKEIF NISLH VTHMGGAPTV NMI+NAPI+EQKPL  +
Sbjct: 246 CLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPLPRE 305

Query: 305 VTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQAKLKS 364
           VT+M+GGAPPPS++LYK++ LGF IVH YGLTETYG AT C WKPEWDSLP +KQAKL S
Sbjct: 306 VTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAKLNS 365

Query: 365 RQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFNGGWF 424
           RQG+QHIG+E  D+K+P+TMESVPADGKTMGEVM+RGNTVM GYLKD+KAT EAFNGGWF
Sbjct: 366 RQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLKDLKATREAFNGGWF 425

Query: 425 RSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPDDHWG 484
           RSGDLGV+HPDGYIELKDRSKDIIISGGENISTIEVESVLF+HPSVL+AA+VGRPDDHWG
Sbjct: 426 RSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDDHWG 485

Query: 485 ETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLILKEEA 544
           ETPCAFV LK G +ATE EIIKFCR++LPHYMAP++VVF+ DLPK+STGK QK ILK+EA
Sbjct: 486 ETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFR-DLPKTSTGKTQKFILKKEA 545

Query: 545 KAMGTLSLAANRL 558
           KAMG+L    ++L
Sbjct: 546 KAMGSLPKRVSKL 555

BLAST of Csa4G658540 vs. TrEMBL
Match: F6I6S6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0122g01440 PE=4 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 3.0e-236
Identity = 397/553 (71.79%), Postives = 467/553 (84.45%), Query Frame = 1

Query: 1   MEG-IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60
           MEG IRCSAN VPL+PI+FLER+A+V+ DR+++VYG V+FTWR+TL+RCT+ ASA+ H+G
Sbjct: 10  MEGMIRCSANHVPLSPISFLERSASVYRDRVAIVYGRVKFTWRDTLERCTRLASAITHLG 69

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120
           ISRGDVVA LAPN+PA YELHF VPMAGA+LCTLN+RHD+AMVSTLL HSEAK+I  D+Q
Sbjct: 70  ISRGDVVAALAPNIPAMYELHFGVPMAGAVLCTLNIRHDSAMVSTLLKHSEAKMIFVDYQ 129

Query: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNI-NGFDSASDDLEYESLLNTGTLD 180
            L   + A+EI+SKT    +LPR+V++ E D     I N   + + DLEYE+L+  G  D
Sbjct: 130 LLDTAQGALEILSKTRT--KLPRLVLIPECDKLQPTISNSGPTVAGDLEYETLIAMGKTD 189

Query: 181 FEIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPM 240
           F+IR P DE DPI+LNYTSGTTSRPKGVIYSHRG +LN+L+ VLLN+M SM VYLWTVPM
Sbjct: 190 FDIRWPNDEWDPIALNYTSGTTSRPKGVIYSHRGAHLNSLAAVLLNEMGSMPVYLWTVPM 249

Query: 241 FHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQ 300
           FHCNGW +TWGVAAQGGTN+C RNV AK IF +IS H VTHMGGAPTVLNMI+NAP+ +Q
Sbjct: 250 FHCNGWCLTWGVAAQGGTNVCLRNVTAKGIFDSISQHRVTHMGGAPTVLNMIINAPVGDQ 309

Query: 301 KPLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEK 360
           +PL GKV VMTGGAPPP  +L+ M+ELGF + H YGLTETYG  T C WKPEWD LP +K
Sbjct: 310 RPLPGKVAVMTGGAPPPPQVLFNMKELGFGVFHSYGLTETYGPGTVCTWKPEWDLLPPDK 369

Query: 361 QAKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEA 420
           QAK+ +RQG+ H+G+E VDIK+P+TM+SVP D KT+GEVM RGNTVM GYLKD+KATEE+
Sbjct: 370 QAKINARQGLHHLGMEEVDIKDPVTMKSVPPDAKTIGEVMFRGNTVMNGYLKDIKATEES 429

Query: 421 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGR 480
           F GGWFRSGDLGV+HPDGYIELKDRSKDIIISGGENISTIEVE+VLF+HP++LEAA+VGR
Sbjct: 430 FKGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVEAVLFSHPAILEAAVVGR 489

Query: 481 PDDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKL 540
           PDDHWGETPCAFV LK G NA   EIIKFCR+ LPHYMAPKTVVF  DLPK+STGK QK 
Sbjct: 490 PDDHWGETPCAFVKLKEGCNANANEIIKFCRNKLPHYMAPKTVVF-YDLPKTSTGKTQKY 549

Query: 541 ILKEEAKAMGTLS 552
           ILKE+AKAMG+LS
Sbjct: 550 ILKEKAKAMGSLS 559

BLAST of Csa4G658540 vs. TrEMBL
Match: M5VIW5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003716mg PE=4 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 2.3e-231
Identity = 395/560 (70.54%), Postives = 462/560 (82.50%), Query Frame = 1

Query: 1   MEG-IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60
           MEG IRCSAN +PL+PI+FLER+A V+ DR S+VYG++ +TWRETL+RCT+ ASAL  +G
Sbjct: 1   MEGSIRCSANYIPLSPISFLERSAIVYRDRPSVVYGSIIYTWRETLERCTRLASALSQLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120
           ISRGDVVA LAPN+PA YELHF VPMAGA+LCTLN+RHD+ MVS LL HS+AK I  D+Q
Sbjct: 61  ISRGDVVAALAPNIPAMYELHFGVPMAGAVLCTLNVRHDSQMVSVLLKHSDAKFIFVDYQ 120

Query: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
             H+ K A +I+SKT    +LP +V++ E D  S  +   +  S +LEYESLL  G LDF
Sbjct: 121 LFHVAKGAFDILSKTRT--KLPVLVLIPESDQSSPEL--CNPISGNLEYESLLGRGKLDF 180

Query: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240
           EIRRP DE DPISLNYTSGTTS PKGVIYSHRG YLN+ + VLLN+M SM VYLW VPMF
Sbjct: 181 EIRRPKDEWDPISLNYTSGTTSSPKGVIYSHRGAYLNSFAAVLLNEMPSMPVYLWCVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGW +TW VAAQGGTNICQRNV AK IF+ IS H VTHMGGAPTVLNMIVNAP  E++
Sbjct: 241 HCNGWCLTWAVAAQGGTNICQRNVTAKGIFSCISQHKVTHMGGAPTVLNMIVNAPENERR 300

Query: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360
           PL GKV VMTGGAPPP+ +L+KM ELGF + H YGLTETYG  T C WKPEWDSLP  +Q
Sbjct: 301 PLPGKVIVMTGGAPPPAQVLFKMEELGFNVTHSYGLTETYGPGTVCSWKPEWDSLPRVEQ 360

Query: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420
           AK+KSRQG+QH+G+E +D+K+P+TM+SVP+D KTMGEVM RGNTVM GYLKD +AT +AF
Sbjct: 361 AKIKSRQGLQHLGMEELDVKDPVTMKSVPSDAKTMGEVMFRGNTVMNGYLKDHQATRDAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
            GGWF SGDLGV+HPDGYIELKDRSKDIIISGGENISTIEVESVLF+HP +LEAAIVGRP
Sbjct: 421 KGGWFHSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPDILEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           D++WGETPCAFV LK G     EEIIKFC+D LPHYMAP+TVVF+ DLPK+STGK+QK +
Sbjct: 481 DEYWGETPCAFVKLKDGCKTGREEIIKFCKDRLPHYMAPRTVVFE-DLPKTSTGKVQKFV 540

Query: 541 LKEEAKAMGTLSLAANRLAK 560
           L+E+AKAMG  SL  N ++K
Sbjct: 541 LREKAKAMG--SLTKNTISK 553

BLAST of Csa4G658540 vs. TrEMBL
Match: B9R8M5_RICCO (AMP dependent ligase, putative OS=Ricinus communis GN=RCOM_1600940 PE=4 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 8.6e-231
Identity = 389/552 (70.47%), Postives = 463/552 (83.88%), Query Frame = 1

Query: 1   MEG-IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60
           MEG I+CSAN VPL+PI+FLER+A  + D IS+VYG+V++TWRET QRC K ASALVH+G
Sbjct: 1   MEGMIKCSANYVPLSPISFLERSAIAYRDSISIVYGDVKYTWRETHQRCIKLASALVHLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120
           I+RGDVVA LAPN+PA YELHF VPMAGA+LCTLN+RHD+AMVS LL HSEAK+I  DHQ
Sbjct: 61  INRGDVVAALAPNIPALYELHFGVPMAGAVLCTLNVRHDSAMVSVLLRHSEAKVIFVDHQ 120

Query: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           +L I + A+EI+SK     +LP  V+VQE   P+       S+S  LE+ESLL  G LDF
Sbjct: 121 FLQIAQGALEILSKL--GTKLPMFVLVQECGQPTPI-----SSSGHLEFESLLAMGKLDF 180

Query: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240
           E+RRP DE DPISLNYTSGTTS PKGVIYSHRG YLN+L+ VLLNDM SM +YLW VPMF
Sbjct: 181 EVRRPNDECDPISLNYTSGTTSSPKGVIYSHRGAYLNSLAAVLLNDMVSMPIYLWCVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGW +TWGVAAQGGT++C R+V+ K IF NI+ + VTHMGGAPTVLNMI+NAP ++QK
Sbjct: 241 HCNGWCLTWGVAAQGGTHVCLRSVSPKGIFENIARYKVTHMGGAPTVLNMIINAPASDQK 300

Query: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360
           PL GKV VMTG APPP  +L+ M ELGF + H YGLTETYG  T C WKPEW SLP + Q
Sbjct: 301 PLPGKVAVMTGAAPPPPRVLFAMEELGFNVTHSYGLTETYGPGTVCTWKPEWTSLPRDVQ 360

Query: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420
           AK+K+RQG+QH+G+E VDIK+P+TM+SVP D KT+GEVM RGNTVM GYLK+++ATEEAF
Sbjct: 361 AKIKARQGLQHLGIEEVDIKDPVTMKSVPPDAKTIGEVMFRGNTVMNGYLKNLRATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
            GGWFRSGDLGV+HPDGYIELKDRSKDIIISGGENISTIEVESVLF+HP+VLEAA+VG P
Sbjct: 421 KGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPAVLEAAVVGSP 480

Query: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFV LK G NA+ +E+IK+CRD+LPHYMAP+TV+F+ DLPK+STGK+QK +
Sbjct: 481 DDHWGETPCAFVKLKDGCNASAQELIKYCRDHLPHYMAPRTVLFE-DLPKTSTGKVQKYV 540

Query: 541 LKEEAKAMGTLS 552
           L+++A A G+LS
Sbjct: 541 LRKKASATGSLS 544

BLAST of Csa4G658540 vs. TrEMBL
Match: B9H5H2_POPTR (AMP-dependent synthetase and ligase family protein OS=Populus trichocarpa GN=POPTR_0005s27210g PE=4 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 1.0e-228
Identity = 384/552 (69.57%), Postives = 464/552 (84.06%), Query Frame = 1

Query: 1   MEG-IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60
           MEG ++CSAN VPLTPI+FLER+A V+ DR+S+ YG++++TW+ET +RC + ASAL H+G
Sbjct: 1   MEGMVKCSANYVPLTPISFLERSAIVYRDRVSVAYGDIKYTWKETHERCVRLASALAHLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120
           IS GDVVA LAPN+PA YELHF VPMAGA+LCTLN+RHD++MVS LL HSEAK+I  D+Q
Sbjct: 61  ISPGDVVAALAPNIPAMYELHFGVPMAGAVLCTLNVRHDSSMVSVLLKHSEAKLIFVDYQ 120

Query: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
            L I + A+EI+S+     +LP +V++ E   P++      S+   LEYESLL  G LDF
Sbjct: 121 CLPIAQGALEILSERKT--KLPLLVLIPECGQPAAI-----SSPRILEYESLLEKGKLDF 180

Query: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240
           E+RRP DE DPISLNYTSGTTS PKGVIYSHRG YLN+L+  LLNDMS+M VYLW VPMF
Sbjct: 181 EVRRPRDEWDPISLNYTSGTTSSPKGVIYSHRGAYLNSLAAALLNDMSAMPVYLWCVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGW +TW VAAQGGTN+CQR V A++IF NI+ H VTHM GAPTVLNMI+NA  +E++
Sbjct: 241 HCNGWCLTWAVAAQGGTNVCQRYVTARDIFENIAQHKVTHMSGAPTVLNMIINASASEKR 300

Query: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360
           PL GKV VMTGGAPPPS++L+KM ELGF + H YGLTETYG  T C WKPEW SL  E Q
Sbjct: 301 PLPGKVAVMTGGAPPPSHVLFKMEELGFHVTHSYGLTETYGPGTVCAWKPEWASLTPEAQ 360

Query: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420
           AK+KSRQG+QH+G+E +DIK+P+TM+SVPAD KTMGEVM RGNTVM GYLK+++AT++AF
Sbjct: 361 AKIKSRQGVQHLGLEELDIKDPVTMKSVPADAKTMGEVMFRGNTVMNGYLKNLEATKDAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           +GGWFRSGDLGV+HPDGYIELKDRSKDIIISGGENIS+IEVES LF+HP+VLEAAIVGRP
Sbjct: 421 SGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESALFSHPAVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DD+WGETPCAFV LK G NA  EE+IKFCRD+LPHYM P+TVVF+ +LPK+STGK+QK +
Sbjct: 481 DDYWGETPCAFVKLKEGCNANAEELIKFCRDHLPHYMTPRTVVFQ-ELPKTSTGKVQKYV 540

Query: 541 LKEEAKAMGTLS 552
           LKE+AKAMG++S
Sbjct: 541 LKEKAKAMGSIS 544

BLAST of Csa4G658540 vs. TAIR10
Match: AT1G20560.1 (AT1G20560.1 acyl activating enzyme 1)

HSP 1 Score: 739.6 bits (1908), Expect = 1.4e-213
Identity = 352/552 (63.77%), Postives = 442/552 (80.07%), Query Frame = 1

Query: 1   MEG-IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60
           MEG I+  AN VPLTPI+FL+R+A V+ DR+S+VYG+V++TWR+T  RC + ASAL  +G
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120
           IS GDVV+VLAPNVPA  ELHF VPMAGA+LCTLN+RHD+++V+ LL HS  K+I ADHQ
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           +L I + A EI+S     +++P +V++ E    + +++    + + +EYE ++  G  DF
Sbjct: 123 FLQIAEGACEILSNK--GDKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDF 182

Query: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240
           E+ RP DE D IS+NYTSGTTS PKGV+YSHRG YLN+L+ VLLN+M S   YLWT PMF
Sbjct: 183 EVIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMF 242

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGW + WGV A GGTNIC RNV AK IF NIS H VTHMGGAPT+LNMI+NAP +EQK
Sbjct: 243 HCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQK 302

Query: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360
           PL GKV+ +TG APPP+++++KM ELGF + H YGLTETYG  T C WKPEWDSLP E+Q
Sbjct: 303 PLPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQ 362

Query: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420
           AK+K+RQG+ H+G+E + +K+P+TM ++PADG TMGEV+ RGNTVM GYLK+ +AT+EAF
Sbjct: 363 AKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAF 422

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
            GGWF SGDLGV+HPDGYIELKDRSKDIIISGGENIS+IEVES LF HP VLEAA+V RP
Sbjct: 423 KGGWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARP 482

Query: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           D++WGET CAFV LK GS A+ EE+I +CRD LPHYMAP+++VF+ DLPK+STGK+QK +
Sbjct: 483 DEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFE-DLPKTSTGKVQKFV 542

Query: 541 LKEEAKAMGTLS 552
           L+ +AKA+ +LS
Sbjct: 543 LRTKAKALVSLS 549

BLAST of Csa4G658540 vs. TAIR10
Match: AT2G17650.1 (AT2G17650.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 708.0 bits (1826), Expect = 4.7e-204
Identity = 344/553 (62.21%), Postives = 425/553 (76.85%), Query Frame = 1

Query: 4   IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRG 63
           +R  AN  PL+PITFLER+A V+ DR SLV+G+V+ TW +T QRC + ASAL ++GISRG
Sbjct: 52  LRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLGISRG 111

Query: 64  DVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHI 123
           DVVA LAPNVPA +ELHFAVPMAG ILC LN R D + +S LL HSEAKI+  DHQ L I
Sbjct: 112 DVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEI 171

Query: 124 VKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSA-----SDDLEYESLLNTGTLD 183
              A+++++K+    +  ++V++ + +    +     S      S D EYE+LL +G  +
Sbjct: 172 AHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSE 231

Query: 184 FEIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPM 243
           FEI +P  E DPIS+NYTSGTTSRPKGV+YSHRG YLN+L+TV L+ MS   VYLWTVPM
Sbjct: 232 FEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLWTVPM 291

Query: 244 FHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQ 303
           FHCNGW + WGVAAQGGTNIC R V+ K IF NI++H VTHMGGAPTVLNMIVN  +TE 
Sbjct: 292 FHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEH 351

Query: 304 KPLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEK 363
           KPL  +V +MTGG+PP   IL KM ELGF + H YGLTETYG  T C WKPEWDSL  E+
Sbjct: 352 KPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEE 411

Query: 364 QAKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEA 423
           + KLK+RQG+QH+G+EG+D+K+P+TME+VP DG TMGEVM RGNTVM GY KD++AT +A
Sbjct: 412 RTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKA 471

Query: 424 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGR 483
           F G WF SGDL V++PDGYIE+KDR KD+IISGGENIS++EVE VL +H +VLEAA+V R
Sbjct: 472 FEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVAR 531

Query: 484 PDDHWGETPCAFVNLKHGSNATE-EEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQK 543
           PD HWG+TPC FV LK G +  + EEII FCRD+LPHYMAPKT+VF  D+PK+STGK+QK
Sbjct: 532 PDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVF-GDIPKTSTGKVQK 591

Query: 544 LILKEEAKAMGTL 551
            +L+++A  MG+L
Sbjct: 592 YLLRKKADEMGSL 603

BLAST of Csa4G658540 vs. TAIR10
Match: AT1G66120.1 (AT1G66120.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 581.6 bits (1498), Expect = 5.1e-166
Identity = 277/551 (50.27%), Postives = 388/551 (70.42%), Query Frame = 1

Query: 4   IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRG 63
           + C AN+VPLTPITFL+RA+  + +R S++YG  RFTW +T  RC + A++L+ + I+R 
Sbjct: 5   VLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLNITRN 64

Query: 64  DVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHI 123
           DVV++LAPNVPA YE+HF+VPM GA+L  +N R DA  ++ +L H+E KI+  D+++  +
Sbjct: 65  DVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYEFAPL 124

Query: 124 VKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTG----TLDF 183
           ++  + ++  T  ++  PRI+++ E D  +         S +L+YE L+  G    +   
Sbjct: 125 IQEVLRLIP-TYQSQPHPRIILINEIDSTTKPF------SKELDYEGLIRKGEPTPSSSA 184

Query: 184 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 243
            + R  +E DPISLNYTSGTT+ PKGV+ SH+G YL+ LS+++  +M    VYLWT+PMF
Sbjct: 185 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMF 244

Query: 244 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 303
           HCNGW+ TW VAA+GGTN+C R+V A EI+ NI LH VTHM   PTV   ++    T+Q 
Sbjct: 245 HCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQS 304

Query: 304 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 363
           P    V V+TGG+ PP+ ++ K+ +LGF ++H YGLTE  G   FC W+ EW+ LP+ +Q
Sbjct: 305 PKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQ 364

Query: 364 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 423
            +L+ RQG++++ +  VD+KN  T+ESVP DGKTMGE++I+G+++M GYLK+ KAT EAF
Sbjct: 365 IELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAF 424

Query: 424 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 483
             GW  +GD+GV HPDGY+E+KDRSKDIIISGGENIS+IEVE VL+ +  VLEAA+V  P
Sbjct: 425 KHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMP 484

Query: 484 DDHWGETPCAFVNLKHGSN---ATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQ 543
              WGETPCAFV LK G      +E ++IK+CR+N+PH+M PK VVF  +LPK+S GKI 
Sbjct: 485 HPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKIL 544

Query: 544 KLILKEEAKAM 548
           K  L++ AKA+
Sbjct: 545 KSKLRDIAKAL 548

BLAST of Csa4G658540 vs. TAIR10
Match: AT1G65880.1 (AT1G65880.1 benzoyloxyglucosinolate 1)

HSP 1 Score: 570.1 bits (1468), Expect = 1.5e-162
Identity = 270/557 (48.47%), Postives = 384/557 (68.94%), Query Frame = 1

Query: 6   CSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRGDV 65
           C AN+VPLTP+TFL+RA+  + +R S++YG  RFTW +T  RC + A++L+ + IS+ DV
Sbjct: 7   CEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNISKNDV 66

Query: 66  VAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHIVK 125
           V+V+APN PA YE+HFAVPMAGA+L  +N R DA  ++ +L H++ KI+  D  +  + +
Sbjct: 67  VSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRSFEALAR 126

Query: 126 AAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTG----TLDFEI 185
            ++ ++S   +   LP ++ + E D P        ++ ++L+YE L+  G    ++   +
Sbjct: 127 ESLHLLSSEDSNLNLP-VIFIHENDFPKR------ASFEELDYECLIQRGEPTPSMVARM 186

Query: 186 RRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFHC 245
            R  DE DPISLNYTSGTT+ PKGV+ SHRG YL TLS ++  +M +  VYLWT+PMFHC
Sbjct: 187 FRIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHC 246

Query: 246 NGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKPL 305
           NGW+ TWG AA+GGT++C R+V A EI+ NI +HNVTHM   PTV N+++     +  P 
Sbjct: 247 NGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPR 306

Query: 306 LGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQAK 365
            G V V+TGG+PPP+ ++ K++ LGF ++H YG TE  G   FC W+ EW+ LP+ +Q +
Sbjct: 307 SGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQME 366

Query: 366 LKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFNG 425
           LK+RQG+  +G+  VD+KN  T +S P DGKTMGE++I+G+++M GYLK+ KAT EAF  
Sbjct: 367 LKARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKH 426

Query: 426 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPDD 485
           GW  +GD+GV HPDG++E+KDRSKDIIISGGENIS++EVE+VL+ +P VLE A+V  P  
Sbjct: 427 GWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHP 486

Query: 486 HWGETPCAFVNLKHGSNATEEE-----------IIKFCRDNLPHYMAPKTVVFKSDLPKS 545
            WGETPCAFV L+      +E+           +I++CR+NLPH+M P+ VVF  +LPK+
Sbjct: 487 TWGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKN 546

Query: 546 STGKIQKLILKEEAKAM 548
             GKI K  L++ AK +
Sbjct: 547 GNGKILKPKLRDIAKGL 556

BLAST of Csa4G658540 vs. TAIR10
Match: AT1G65890.1 (AT1G65890.1 acyl activating enzyme 12)

HSP 1 Score: 567.0 bits (1460), Expect = 1.3e-161
Identity = 269/556 (48.38%), Postives = 382/556 (68.71%), Query Frame = 1

Query: 6   CSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRGDV 65
           C AN+VPLTPITFL+RA+  + +R S++YG  RFTW +T  RC + A++L+ + I + DV
Sbjct: 7   CEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNIGKNDV 66

Query: 66  VAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHIVK 125
           V+V+APN PA YE+HFAVPMAGA+L  +N R DA  ++ +L H++ KI+     +  + +
Sbjct: 67  VSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRSFEPLAR 126

Query: 126 AAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGT-LDFEIRRP 185
             ++++S   +   LP ++ + E D P         +S++ +YE L+  G      + R 
Sbjct: 127 EILQLLSSEDSNLNLP-VIFIHEIDFPKR------VSSEESDYECLIQRGEPTPLLLARM 186

Query: 186 I---DERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFHC 245
               DE DPISLNYTSGTT+ PKGV+ SHRG YL+TLS ++  +M +  VYLWT+PMFHC
Sbjct: 187 FCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHC 246

Query: 246 NGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKPL 305
           NGW+ TWG AA+GGT++C R+V A EI+ NI +HNVTHM   PTV N+++     +    
Sbjct: 247 NGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHR 306

Query: 306 LGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQAK 365
            G V V+TGG+PPP+ ++ K++ LGF ++H YGLTE  G   FC W+ EW+ LP+ +Q +
Sbjct: 307 SGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQME 366

Query: 366 LKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFNG 425
           LK+RQG+  +G+  VD++N  T ESVP DGKTMGE++++G+++M GYLK+ KAT EAF  
Sbjct: 367 LKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKH 426

Query: 426 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPDD 485
           GW  SGD+GV HPDG++E+KDRSKDIIISGGENIS++EVE++++ +P VLE A+V  P  
Sbjct: 427 GWLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHP 486

Query: 486 HWGETPCAFVNLKHGSNATEE----------EIIKFCRDNLPHYMAPKTVVFKSDLPKSS 545
            WGETPCAFV L+ G    E+          ++I++CR+NLPH+M P+ VVF  +LPK+ 
Sbjct: 487 TWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNG 546

Query: 546 TGKIQKLILKEEAKAM 548
            GKI K  L++ AK +
Sbjct: 547 NGKILKPKLRDIAKGL 555

BLAST of Csa4G658540 vs. NCBI nr
Match: gi|449455718|ref|XP_004145598.1| (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus])

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 559/559 (100.00%), Postives = 559/559 (100.00%), Query Frame = 1

Query: 1   MEGIRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGI 60
           MEGIRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGI
Sbjct: 1   MEGIRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGI 60

Query: 61  SRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQY 120
           SRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQY
Sbjct: 61  SRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQY 120

Query: 121 LHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDFE 180
           LHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDFE
Sbjct: 121 LHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDFE 180

Query: 181 IRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFH 240
           IRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFH
Sbjct: 181 IRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFH 240

Query: 241 CNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKP 300
           CNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKP
Sbjct: 241 CNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKP 300

Query: 301 LLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQA 360
           LLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQA
Sbjct: 301 LLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQA 360

Query: 361 KLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFN 420
           KLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFN
Sbjct: 361 KLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFN 420

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPD 480
           GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPD
Sbjct: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPD 480

Query: 481 DHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLIL 540
           DHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLIL
Sbjct: 481 DHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLIL 540

Query: 541 KEEAKAMGTLSLAANRLAK 560
           KEEAKAMGTLSLAANRLAK
Sbjct: 541 KEEAKAMGTLSLAANRLAK 559

BLAST of Csa4G658540 vs. NCBI nr
Match: gi|659104647|ref|XP_008452948.1| (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo])

HSP 1 Score: 1062.4 bits (2746), Expect = 2.8e-307
Identity = 526/557 (94.43%), Postives = 537/557 (96.41%), Query Frame = 1

Query: 1   MEGI-RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60
           MEGI  CSANS+PLTPITFLERAAAVF DRISLVYGNVRFTW ETLQRCTKFASALVH+G
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120
           ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAM+STLLGHSEAKIIV DHQ
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           YLHIVKAAIEIMSK   AEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240
           EIRRPIDE DPISLNYTSGTTSRPKGVIYSHRGTYLNTLST+LLNDMSSM VYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLH VTHMGGAPTVLNMIVNAPITEQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360
           PL GKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTET GVATFC WKPEWDSLPD+KQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420
           AKLKSRQGMQHIGVEG+DIKNPITMESVPADGKTMGEVMIRGNTVM GYLKD+KATEEAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLK GSNAT+EEII+FCRD LPHYMAPKTVVFKSDLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMGTLSLAANR 557
           LKEEAKAMG+LS A  +
Sbjct: 541 LKEEAKAMGSLSPAKRK 557

BLAST of Csa4G658540 vs. NCBI nr
Match: gi|449455591|ref|XP_004145536.1| (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus])

HSP 1 Score: 877.1 bits (2265), Expect = 1.6e-251
Identity = 425/553 (76.85%), Postives = 485/553 (87.70%), Query Frame = 1

Query: 5   RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRGD 64
           RCSAN VPLTPI+FLER+AAV+GDRISLVYG V++TWR+TLQRCT+ ASALV  GI+RGD
Sbjct: 6   RCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTGIARGD 65

Query: 65  VVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHIV 124
           VVA L PN+PA YELHFAVPMAGA+LC+LN RHDAAMVSTLL HSEAKIIV D+Q  HIV
Sbjct: 66  VVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQLEHIV 125

Query: 125 KAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDFEIRRP 184
             AI+ MS+    E+LPR+VI+QE+D P S I+   SA + LE+ES L +G L+FEIRRP
Sbjct: 126 TGAIKAMSERK--EKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEIRRP 185

Query: 185 IDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFHCNGW 244
            DE DPI+LNYTSGTTSRPKGVI+SHRG YLN+LS VLLNDM S+ VYLWTVPMFHCNGW
Sbjct: 186 RDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGW 245

Query: 245 SMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKPLLGK 304
            +TWGVAAQ GTNICQRNV AKEIF NISLH VTHMGGAPTV NMI+NAPI+EQKPL  +
Sbjct: 246 CLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPLPRE 305

Query: 305 VTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQAKLKS 364
           VT+M+GGAPPPS++LYK++ LGF IVH YGLTETYG AT C WKPEWDSLP +KQAKL S
Sbjct: 306 VTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAKLNS 365

Query: 365 RQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFNGGWF 424
           RQG+QHIG+E  D+K+P+TMESVPADGKTMGEVM+RGNTVM GYLKD+KAT EAFNGGWF
Sbjct: 366 RQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLKDLKATREAFNGGWF 425

Query: 425 RSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPDDHWG 484
           RSGDLGV+HPDGYIELKDRSKDIIISGGENISTIEVESVLF+HPSVL+AA+VGRPDDHWG
Sbjct: 426 RSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDDHWG 485

Query: 485 ETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLILKEEA 544
           ETPCAFV LK G +ATE EIIKFCR++LPHYMAP++VVF+ DLPK+STGK QK ILK+EA
Sbjct: 486 ETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFR-DLPKTSTGKTQKFILKKEA 545

Query: 545 KAMGTLSLAANRL 558
           KAMG+L    ++L
Sbjct: 546 KAMGSLPKRVSKL 555

BLAST of Csa4G658540 vs. NCBI nr
Match: gi|659104645|ref|XP_008452947.1| (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo])

HSP 1 Score: 874.4 bits (2258), Expect = 1.1e-250
Identity = 428/558 (76.70%), Postives = 488/558 (87.46%), Query Frame = 1

Query: 1   MEGI-RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60
           M+GI RCSAN VPLTPITFLER+AAV+GDRISLVYG V++TWR+TL+RCT+ ASALV  G
Sbjct: 1   MDGIHRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRDTLERCTRLASALVRTG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120
           I+RGDVVA L PN+PA YELHFAVPMAGA+LC+LN RHDAAMVSTLL HSEAKIIV D+Q
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
             HIV  AI  MS+    E+LPR+VI+QE+D P S+I+   SA   LE+ES L +G LDF
Sbjct: 121 LEHIVTGAINTMSERK--EKLPRVVIIQEYDQPPSHIDRPGSA---LEFESFLASGELDF 180

Query: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240
           EIRRP DE DPI+LNYTSGTTSRPKGVIYSHRG YLN LS VLLNDM S+ VYLWTVPMF
Sbjct: 181 EIRRPRDEWDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGW +TWGVAAQ GTNICQRNV AKEIF NISLH VTHMGGAPTV NMI+NAPI+EQK
Sbjct: 241 HCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQK 300

Query: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360
           PL  +VT+M+GGAPPPS++LYK++ LGF IVH YGLTETYG  T C WKPEWDSLP +KQ
Sbjct: 301 PLPRQVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPGTVCSWKPEWDSLPQDKQ 360

Query: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420
           AKL SRQG+QHIG+E VDIK+ +TM+SVPADGKTMGEVM+RGNTVM GYLKD+KAT+EAF
Sbjct: 361 AKLNSRQGLQHIGLEAVDIKDSVTMKSVPADGKTMGEVMLRGNTVMSGYLKDLKATQEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGV+HPDGYIELKDRSKDIIISGGENISTIEVESVLF+HPSVL+AA+VGRP
Sbjct: 421 NGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRP 480

Query: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAF+ LK G +ATEEEIIKFCR++LPHYMAP++VVF+ +LPK+STGK QK I
Sbjct: 481 DDHWGETPCAFLKLKDGCSATEEEIIKFCREHLPHYMAPRSVVFR-ELPKTSTGKTQKFI 540

Query: 541 LKEEAKAMGTLSLAANRL 558
           LK+EAKAMG+L    ++L
Sbjct: 541 LKKEAKAMGSLPKRVSKL 552

BLAST of Csa4G658540 vs. NCBI nr
Match: gi|225457528|ref|XP_002270022.1| (PREDICTED: probable acyl-activating enzyme 1, peroxisomal isoform X1 [Vitis vinifera])

HSP 1 Score: 825.9 bits (2132), Expect = 4.4e-236
Identity = 397/553 (71.79%), Postives = 467/553 (84.45%), Query Frame = 1

Query: 1   MEG-IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60
           MEG IRCSAN VPL+PI+FLER+A+V+ DR+++VYG V+FTWR+TL+RCT+ ASA+ H+G
Sbjct: 10  MEGMIRCSANHVPLSPISFLERSASVYRDRVAIVYGRVKFTWRDTLERCTRLASAITHLG 69

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120
           ISRGDVVA LAPN+PA YELHF VPMAGA+LCTLN+RHD+AMVSTLL HSEAK+I  D+Q
Sbjct: 70  ISRGDVVAALAPNIPAMYELHFGVPMAGAVLCTLNIRHDSAMVSTLLKHSEAKMIFVDYQ 129

Query: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNI-NGFDSASDDLEYESLLNTGTLD 180
            L   + A+EI+SKT    +LPR+V++ E D     I N   + + DLEYE+L+  G  D
Sbjct: 130 LLDTAQGALEILSKTRT--KLPRLVLIPECDKLQPTISNSGPTVAGDLEYETLIAMGKTD 189

Query: 181 FEIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPM 240
           F+IR P DE DPI+LNYTSGTTSRPKGVIYSHRG +LN+L+ VLLN+M SM VYLWTVPM
Sbjct: 190 FDIRWPNDEWDPIALNYTSGTTSRPKGVIYSHRGAHLNSLAAVLLNEMGSMPVYLWTVPM 249

Query: 241 FHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQ 300
           FHCNGW +TWGVAAQGGTN+C RNV AK IF +IS H VTHMGGAPTVLNMI+NAP+ +Q
Sbjct: 250 FHCNGWCLTWGVAAQGGTNVCLRNVTAKGIFDSISQHRVTHMGGAPTVLNMIINAPVGDQ 309

Query: 301 KPLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEK 360
           +PL GKV VMTGGAPPP  +L+ M+ELGF + H YGLTETYG  T C WKPEWD LP +K
Sbjct: 310 RPLPGKVAVMTGGAPPPPQVLFNMKELGFGVFHSYGLTETYGPGTVCTWKPEWDLLPPDK 369

Query: 361 QAKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEA 420
           QAK+ +RQG+ H+G+E VDIK+P+TM+SVP D KT+GEVM RGNTVM GYLKD+KATEE+
Sbjct: 370 QAKINARQGLHHLGMEEVDIKDPVTMKSVPPDAKTIGEVMFRGNTVMNGYLKDIKATEES 429

Query: 421 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGR 480
           F GGWFRSGDLGV+HPDGYIELKDRSKDIIISGGENISTIEVE+VLF+HP++LEAA+VGR
Sbjct: 430 FKGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVEAVLFSHPAILEAAVVGR 489

Query: 481 PDDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKL 540
           PDDHWGETPCAFV LK G NA   EIIKFCR+ LPHYMAPKTVVF  DLPK+STGK QK 
Sbjct: 490 PDDHWGETPCAFVKLKEGCNANANEIIKFCRNKLPHYMAPKTVVF-YDLPKTSTGKTQKY 549

Query: 541 ILKEEAKAMGTLS 552
           ILKE+AKAMG+LS
Sbjct: 550 ILKEKAKAMGSLS 559