Csa4G287530 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa4G287530
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionLipoxygenase; contains IPR000907 (Lipoxygenase), IPR008976 (Lipase/lipooxygenase, PLAT/LH2), IPR027433 (Lipoxygenase, domain 3)
LocationChr4 : 11158737 .. 11161115 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGTTGAGGTTATTGCTTCTGTTACTGTGAAACCCAAAACGAAGGGACCTACTTATCCTTACGCAAACATCATTCTCAAGTTTGCTAGCATTGAATTGGATTCGGGTATGTCATTCTTTATGTTTGTTAGATCATATAATATTAAATTTATATTAGTAAAGTGTTGTATATGTTTTAAGTAAGGTTATAAGGTTTTGGGTGAATTGATGATTGAATAATAATAATGATATGTAAATGGATTTGATGTACACATAGATCAACAACAAAAGCCATTTATCAAATGTGTGGTGGAACCAATACTGCCTGATGATGAAACTGAGGAGTACAAGAAGTACGTAGGTGAAGTTGAGGTTCCAGAAGGTTATGGGGAGATTGGAGCAGTGATTGTTGAATTGGAAGGTGATACGACCAAGTATGAAAAATTTATTGACACTATTTCTATTACGGACAAAAAGTCTAGGAACTCAACTACGTTCAGTTGCAAATCATGGGTGCAATCCAAATCGGTTCTTGACCAGCGAAGGGTCTTCTTTTCCACCAAGGTTCATATATATAATTAAAATCTTCTTTCACTCATGAGCTTAACTTTTTACGTCTAGAATACCATGTGTTTAAACTCTCCATCAAACTTTTCGATCAATTTGTGATTTAAGAATAATTAAGTTATTTAAATTTATTCTGATGAGTTAATAGAATAATTAAGTTATTTAAATTTATTCTAATGAGTTAATAGTGGAAATGGTTGACAGTCGTATTTACCGGAAAGAACACCAGGCGGATTGTTGAAGCTGAGAGCAGAAGACCTTTCGAATCTAAGAGGCATAAAAGCAGATGAAACAGTGGATATGAATGAGCGTAAGGCATTCGAAAGAATTTATGATTACGACTTCTACAATGACCTTGGAGATTGTGATGGTCCCGCAGAATGGAAAAGGCCTGTTCTTGGTGGTTCCGATCACCCTTATCCTCGTCGTTGTAGAACGGGTCGTCTTCATTGTAAAACAGGTATCTTCTGTTTATGTTTTTTATGTATTTAAAATTTCTTTCTAAAATTTTTGTTCATTAAAAACAAAGGAAAATATGATAATAATATGGTTGCATTTTGTGAAATGGTAACTATTAGATCCGGTCTCAGAGAAAAGGAGTAAAGAAAGGTTTTATGTTCCAAGAGATGAAGAATTCTCAGAGGTGAAGCAACATTATTTCCCTTCAAGTGAACCAGATAACAAGGATTTATTGGGAAAAGATTCTTTCTCTGACCTCCCTCATATTGAGTCAATGTTTAGAGAAGGAATTAAAGCCCCTCATGCTCCTCACAAACTTCTCACGTTTAATTTATCAACCATAGTTACCCCAGACAAACCTGACCTTCATTCCAATTCCAATTCATCTGTGCCCAGTTCTCTCCTTCATTATCCACCACCAGAGTCCTACAGGAGTAAGTAATTATATATATATTTTTTCTTTTTATGTTAATTGGAACTTTTTAAAGTGTGCCAATTGTAGCATTTGCCTTACTAATCCTTATAAATGTTTGGTTATTTTTCTCAGGAGATAGGTACAGTTGGCTCAGTGATACCGAATTTGCAAGACAGACACTGGCTGGTCTTAATCCTTACTCCATACAGCTTGTCACAGTAAGTGGCGATCGATAATATATTGGCCTCTAATTAGGGGTACTGTATATGGGTTGAGAGAATTTTTTAGATTAATTTCACAATCCAACCATACCATTTGAGTTGGGTCGTAGTTCAAGTTGAAATATCATTATTTTTATATCACTTCTTTATTGTCCTAACAAATGCAGCGTTTGCCGTTAATGAGTGAGCTTGACCCAGATACTTATGGACCTCAAGAATCAGCTTTCAACCACACAAAGGTTCAAGAATTAATCGGATGTTCCATCGAAGTTAACGAGGTAAATTAAATTAAATCCAACTTACTAATTTAGTAAATTATTAGAAGCGAAAAAGAAAATGATGATCTTTATTATATAAAGTATACTTATTTCTGTGAGTTTGTGCAGGCAATAGAATTGAAAAGATTATTCGTATTGGATTACCATGATACTTTAATGCCATACGTAAACAAAGTAAGAAAAATTAACGGGACGACATTATATGGATCAAGGACATTGTTCTTCTTGAAGTCGGATGACACTTTGATTCCATTAGGTATTGAGCTGACTCGACCGCCAGTGGATGGTTATCCCCAGTGGAAACAAATTTTTACACCAGGCATTGAGGCCACAGATTTGTGGCTATGGAGAATTGCCAAAGCTCATGTCCTTGCTCATGATTCTTGTATTCACCAACTTGTTATCCACTGGTACTTACGTTATTTATCCATACATTCATGGAAAGAAAAAGAAGTAA

mRNA sequence

ATGGGTGTTGAGGTTATTGCTTCTGTTACTGTGAAACCCAAAACGAAGGGACCTACTTATCCTTACGCAAACATCATTCTCAAGTTTGCTAGCATTGAATTGGATTCGGATCAACAACAAAAGCCATTTATCAAATGTGTGGTGGAACCAATACTGCCTGATGATGAAACTGAGGAGTACAAGAAGTACGTAGGTGAAGTTGAGGTTCCAGAAGGTTATGGGGAGATTGGAGCAGTGATTGTTGAATTGGAAGGTGATACGACCAAGTATGAAAAATTTATTGACACTATTTCTATTACGGACAAAAAGTCTAGGAACTCAACTACGTTCAGTTGCAAATCATGGGTGCAATCCAAATCGGTTCTTGACCAGCGAAGGGTCTTCTTTTCCACCAAGAAAAGGAGTAAAGAAAGGTTTTATGTTCCAAGAGATGAAGAATTCTCAGAGGTGAAGCAACATTATTTCCCTTCAAGTGAACCAGATAACAAGGATTTATTGGGAAAAGATTCTTTCTCTGACCTCCCTCATATTGAGTCAATGTTTAGAGAAGGAATTAAAGCCCCTCATGCTCCTCACAAACTTCTCACGTTTAATTTATCAACCATAGTTACCCCAGACAAACCTGACCTTCATTCCAATTCCAATTCATCTGTGCCCAGTTCTCTCCTTCATTATCCACCACCAGAGTCCTACAGGAGAGATAGGTACAGTTGGCTCAGTGATACCGAATTTGCAAGACAGACACTGGCTGGTCTTAATCCTTACTCCATACAGCTTGTCACACGTTTGCCGTTAATGAGTGAGCTTGACCCAGATACTTATGGACCTCAAGAATCAGCTTTCAACCACACAAAGGTTCAAGAATTAATCGGATGTTCCATCGAAGTTAACGAGTGGATGGTTATCCCCAGTGGAAACAAATTTTTACACCAGGCATTGAGGCCACAGATTTGTGGCTATGGAGAATTGCCAAAGCTCATGTCCTTGCTCATGATTCTTGTATTCACCAACTTGTTATCCACTGGTACTTACGTTATTTATCCATACATTCATGGAAAGAAAAAGAAGTAA

Coding sequence (CDS)

ATGGGTGTTGAGGTTATTGCTTCTGTTACTGTGAAACCCAAAACGAAGGGACCTACTTATCCTTACGCAAACATCATTCTCAAGTTTGCTAGCATTGAATTGGATTCGGATCAACAACAAAAGCCATTTATCAAATGTGTGGTGGAACCAATACTGCCTGATGATGAAACTGAGGAGTACAAGAAGTACGTAGGTGAAGTTGAGGTTCCAGAAGGTTATGGGGAGATTGGAGCAGTGATTGTTGAATTGGAAGGTGATACGACCAAGTATGAAAAATTTATTGACACTATTTCTATTACGGACAAAAAGTCTAGGAACTCAACTACGTTCAGTTGCAAATCATGGGTGCAATCCAAATCGGTTCTTGACCAGCGAAGGGTCTTCTTTTCCACCAAGAAAAGGAGTAAAGAAAGGTTTTATGTTCCAAGAGATGAAGAATTCTCAGAGGTGAAGCAACATTATTTCCCTTCAAGTGAACCAGATAACAAGGATTTATTGGGAAAAGATTCTTTCTCTGACCTCCCTCATATTGAGTCAATGTTTAGAGAAGGAATTAAAGCCCCTCATGCTCCTCACAAACTTCTCACGTTTAATTTATCAACCATAGTTACCCCAGACAAACCTGACCTTCATTCCAATTCCAATTCATCTGTGCCCAGTTCTCTCCTTCATTATCCACCACCAGAGTCCTACAGGAGAGATAGGTACAGTTGGCTCAGTGATACCGAATTTGCAAGACAGACACTGGCTGGTCTTAATCCTTACTCCATACAGCTTGTCACACGTTTGCCGTTAATGAGTGAGCTTGACCCAGATACTTATGGACCTCAAGAATCAGCTTTCAACCACACAAAGGTTCAAGAATTAATCGGATGTTCCATCGAAGTTAACGAGTGGATGGTTATCCCCAGTGGAAACAAATTTTTACACCAGGCATTGAGGCCACAGATTTGTGGCTATGGAGAATTGCCAAAGCTCATGTCCTTGCTCATGATTCTTGTATTCACCAACTTGTTATCCACTGGTACTTACGTTATTTATCCATACATTCATGGAAAGAAAAAGAAGTAA

Protein sequence

MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEYKKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVLDQRRVFFSTKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEWMVIPSGNKFLHQALRPQICGYGELPKLMSLLMILVFTNLLSTGTYVIYPYIHGKKKK*
Homology
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BLAST of Csa4G287530 vs. Swiss-Prot
Match: LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 7.0e-22
Identity = 71/198 (35.86%), Postives = 98/198 (49.49%), Query Frame = 1 

Query: 140 YVPRDEEFSEVKQHYFPSSE---------PDNKDLLGKD-SFSDLPHIESMFREGIKAPH 199
           YVPRDE+FS+VK   F ++          P    LL     FS  P I++++ +GI  P 
Sbjct: 292 YVPRDEQFSDVKGRTFSATTLRSGLHAILPAVAPLLNNSHGFSHFPAIDALYSDGIPLPV 351

Query: 200 APHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTL 259
             H   +FN+   V P    +  ++   V    L +  PE   RDR+SW  D EFARQTL
Sbjct: 352 DGHGGNSFNVINDVIPRVVQMIEDTTEHV----LRFEVPEMLERDRFSWFRDEEFARQTL 411

Query: 260 AGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEWMVIPSGNKFL 319
           AGLNP  I+ +T  P++S+LDP  YGP ESA +   +++++   + V E M       FL
Sbjct: 412 AGLNPICIRRLTEFPIVSKLDPAVYGPAESALSKEILEKMMNGRMTVEEAM--EKKRLFL 471

Query: 320 ---HQALRPQICGYGELP 325
              H    P +    ELP
Sbjct: 472 LDYHDVFLPYVHRVRELP 483

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BLAST of Csa4G287530 vs. Swiss-Prot
Match: LOXC1_ORYSJ (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 97.8 bits (242), Expect = 2.5e-19
Identity = 62/177 (35.03%), Postives = 90/177 (50.85%), Query Frame = 1 

Query: 136 KERFYVPRDEEFSEVKQHYF---------PSSEPDNKDLLGKDSFSDLPH-----IESMF 195
           K   YVPRDEEFS  K+ YF          ++ P  + LL      +LP      I+ +F
Sbjct: 312 KGNVYVPRDEEFSPEKEDYFLRKTVGSVLQAAVPAAQSLLLDKLKWNLPFPSFFVIDKLF 371

Query: 196 REGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSD 255
            +G++ P      L F  S +     P L  +   +    +L +  P + ++D+++WL D
Sbjct: 372 EDGVELPGVDK--LNFLESVV-----PRLLEHLRDTPAEKILRFETPANIQKDKFAWLRD 431

Query: 256 TEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE 299
            EFAR+TLAG+NPY+I+LV   PL S+LDP  YGP ESA     ++E +   + V E
Sbjct: 432 EEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEE 481

[Close]
BLAST of Csa4G287530 vs. Swiss-Prot
Match: LOX21_SOLTU (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.3e-19
Identity = 78/235 (33.19%), Postives = 105/235 (44.68%), Query Frame = 1 

Query: 85  GDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVLDQRRVFFSTKKRSKER------ 144
           G+   +E+  D     D    +S     K  V     L   R   + + RSK+       
Sbjct: 230 GERKVFERIYDYDVYNDLGEADSNNDDAKRPVLGGKELPYPRRCKTGRPRSKKDPLSETR 289

Query: 145 ---FYVPRDEEFSEVKQHYFPSSE---------PDNKDLLGKDS--FSDLPHIESMFREG 204
               YVPRDE FSEVK   F  +          P  + ++   +  F   P I+S+F  G
Sbjct: 290 STFVYVPRDEAFSEVKSVAFSGNTVYSVLHAVVPALESVVTDPNLGFPHFPAIDSLFNVG 349

Query: 205 IKAPHAPHKLL-TFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTE 264
           +  P    K    FN    V P      S++   V    L +  P+  +RD++SW  D E
Sbjct: 350 VDLPGLGDKKSGLFN----VVPRLIKAISDTRKDV----LLFESPQLVQRDKFSWFRDVE 409

Query: 265 FARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE 299
           FARQTLAGLNPYSI+LVT  PL S+LDP  YGP ES      +++ IG  + V +
Sbjct: 410 FARQTLAGLNPYSIRLVTEWPLRSKLDPKVYGPPESEITKELIEKEIGNYMTVEQ 456


HSP 2 Score: 44.7 bits (104), Expect = 2.5e-03
Identity = 22/70 (31.43%), Postives = 33/70 (47.14%), Query Frame = 1 

Query: 63  YVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVL 122
           Y  +  +P+ +GE+GA+++E E     Y K I    + D         +C SWV SK   
Sbjct: 137 YEADFVIPQDFGEVGAILIENEHHKEMYVKNI----VIDGFVHGKVEITCNSWVHSKFDN 196

Query: 123 DQRRVFFSTK 133
             +R+FF+ K
Sbjct: 197 PDKRIFFTNK 202

[Close]
BLAST of Csa4G287530 vs. Swiss-Prot
Match: LOX2_ARATH (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.3e-19
Identity = 67/180 (37.22%), Postives = 88/180 (48.89%), Query Frame = 1 

Query: 130 STKKRSKERFYVPRDEEFSEVKQHYF---------PSSEPDNKDLL--GKDSFSDLPHIE 189
           S+++R    FYVPRDEEFS  K   F         PS  P  + +L   ++ F     I+
Sbjct: 286 SSEQRYGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQ 345

Query: 190 SMFREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSW 249
           ++F EGI+ P     L          P  P +   +       +L +  P    RDR+SW
Sbjct: 346 NLFEEGIQLPKDAGLL----------PLLPRI-IKALGEAQDDILQFDAPVLINRDRFSW 405

Query: 250 LSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE 299
           L D EFARQTLAGLNPYSIQLV   PL+S+LDP  YG   S      V+  +  ++ V+E
Sbjct: 406 LRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDE 454


HSP 2 Score: 50.4 bits (119), Expect = 4.6e-05
Identity = 31/110 (28.18%), Postives = 54/110 (49.09%), Query Frame = 1 

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           +KY  E E+PE +G +GA+ ++      +Y + +    +  K    S TF+C+SWV  KS
Sbjct: 135 EKYECEFEMPEDFGPVGAIKIQ-----NQYHRQLFLKGVELKLPGGSITFTCESWVAPKS 194

Query: 121 VLDQRRVFFSTKKRSKERFYVPRD--EEFSEVKQHYFPSSEPDNKDLLGK 169
           V   +R+FFS K       Y+P    E   + ++    + +  N++ +G+
Sbjct: 195 VDPTKRIFFSDKS------YLPSQTPEPLKKYRKEELETLQGKNREEVGE 233

[Close]
BLAST of Csa4G287530 vs. Swiss-Prot
Match: LOXC2_ORYSJ (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.6e-18
Identity = 65/177 (36.72%), Postives = 92/177 (51.98%), Query Frame = 1 

Query: 136 KERFYVPRDEEFSEVKQHYF---------PSSEPDNKDLLGKDSFSDLPH-----IESMF 195
           K   YVPRDEEFSEVK   F          ++ P  +  L  +   +LP      I+ +F
Sbjct: 329 KGNVYVPRDEEFSEVKNAQFLLKTLQSVLHAAVPAAQSALIDNLSLNLPFPSFFVIDKLF 388

Query: 196 REGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSD 255
            +G++ P    + L F L +IV    P L      S    +L +  P + ++D+++WL D
Sbjct: 389 EDGVELPGV--EKLGF-LHSIV----PRLLELLRDSPGDKILLFDTPANVQKDKFAWLRD 448

Query: 256 TEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE 299
            EFAR+TLAG+NPY+I+LV   PL S+LDP  YGP ESA     ++E +   + V E
Sbjct: 449 EEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEE 498

[Close]
BLAST of Csa4G287530 vs. TrEMBL
Match: A0A0A0L1V5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G287530 PE=4 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 4.7e-206
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 1 

Query: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60
           MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY
Sbjct: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS
Sbjct: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120

Query: 121 VLDQRRVFFSTKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESM 180
           VLDQRRVFFSTKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESM
Sbjct: 121 VLDQRRVFFSTKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESM 180

Query: 181 FREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLS 240
           FREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLS
Sbjct: 181 FREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLS 240

Query: 241 DTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEWM 300
           DTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEWM
Sbjct: 241 DTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEWM 300

Query: 301 VIPSGNKFLHQALRPQICGYGELPKLMSLLMILVFTNLLSTGTYVIYPYIHGKKKK 357
           VIPSGNKFLHQALRPQICGYGELPKLMSLLMILVFTNLLSTGTYVIYPYIHGKKKK
Sbjct: 301 VIPSGNKFLHQALRPQICGYGELPKLMSLLMILVFTNLLSTGTYVIYPYIHGKKKK 356

[Close]
BLAST of Csa4G287530 vs. TrEMBL
Match: A0A0A0KX68_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G286960 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.0e-71
Identity = 163/315 (51.75%), Postives = 209/315 (66.35%), Query Frame = 1 

Query: 1   MGVEVIASVTVKPKTKGPTYPYAN-IILKFASIELDSDQQQKPFIKCVVEPILPDDETEE 60
           M   +IA V+V PK    T+P  + ++L FAS +LD D QQKP I  V E I  +   E+
Sbjct: 1   MASNIIAIVSVIPKIN-ETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEK 60

Query: 61  YKKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSK 120
           YK YV +++VP+G+GEIGAVIVEL+ D++  EKFIDT+ + +  S N+ TFSC SWVQ K
Sbjct: 61  YK-YVAKIQVPKGFGEIGAVIVELKDDSS--EKFIDTVIVANPTSHNTITFSCTSWVQPK 120

Query: 121 SVL-DQRRVFFSTK------------KRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLL 180
           S++ DQRRVFFSTK            +RS E FYVPRDE FSEVKQ  FPS+ P  KD L
Sbjct: 121 SLIPDQRRVFFSTKSNIEIDFDPKSEQRSTESFYVPRDEAFSEVKQKMFPSN-PGKKDKL 180

Query: 181 GKDSFSDLPHIESMFREGIKAPHAPHKLLTFNL-STIVTPDKPDLHSNSNSSVPSS--LL 240
           G   F+D   I+ MFR+GI  P  PH++L FN+ ST+   ++P         V  +  ++
Sbjct: 181 GAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPPVQPIPVQPNTPVI 240

Query: 241 HYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNH 299
            +PPPE+ +RD+++WLSD+EFARQT+AGLNPY IQLV   PL SELDP  YGPQESAF  
Sbjct: 241 KFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELDPVEYGPQESAFTT 300

[Close]
BLAST of Csa4G287530 vs. TrEMBL
Match: A0A0A0KZ89_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G287020 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.1e-65
Identity = 168/353 (47.59%), Postives = 210/353 (59.49%), Query Frame = 1 

Query: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60
           M  +   +VTV PK          I L+F S+ELDS   Q+  I C        D +   
Sbjct: 1   MSYKGTVTVTVNPKKNEAFLTIEVIYLEFVSVELDSKGNQQ-IIGCTAGLKCGGDVS--- 60

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           K +   ++VP  +GEIGAVIVEL+ D    E+FIDTI+I  KK     TFSCKSWVQ K+
Sbjct: 61  KLFETSIDVPGDFGEIGAVIVELDDDVK--ERFIDTITIDFKKC-TLLTFSCKSWVQPKN 120

Query: 121 VLDQRRVFFSTKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESM 180
              + R+        +  FYVPRDE FSE KQ    S   +N+ LLGK  FSDLP ++  
Sbjct: 121 KHAEERI--------EAPFYVPRDENFSEEKQG---SVLAENEALLGKAPFSDLPELD-- 180

Query: 181 FREGIKAPHAPHKL--LTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSW 240
               IK P A  K+  L F++ ++V+ ++P    NS    PSS +  P PESY+RD+Y+W
Sbjct: 181 ----IKTPFAASKINQLPFDIPSLVSSNQPP-PLNSLDDEPSSSVELPLPESYKRDKYNW 240

Query: 241 LSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE 300
           LSD EFAR TLAGLNPYSIQLV  LP MS+LD   YGP+ES F H  VQEL+GC I+V +
Sbjct: 241 LSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTHELVQELLGCRIKVCK 300

Query: 301 WMVIPSGNKFLHQALRPQICGYGELPKLMSLLMILVFTNLLSTGTYVIYPYIH 352
             ++ +G KF HQ L   ICGYG L K MS L IL F NLL TG Y I+ YIH
Sbjct: 301 -DILNNGKKFSHQVLIQPICGYGGLRKPMSFLTILAFINLLFTGMY-IHIYIH 326

[Close]
BLAST of Csa4G287530 vs. TrEMBL
Match: A0A0A0KX73_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G287550 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.3e-58
Identity = 133/269 (49.44%), Postives = 174/269 (64.68%), Query Frame = 1 

Query: 36  SDQQQKPFIKCVVEPILPDDETEEYKKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFID 95
           +D+Q K FIKC  + +   DE+ EYKKY G+++V EG+G+IGAVIVEL+      E+F+D
Sbjct: 20  ADEQPKEFIKCEAQ-LQEVDESVEYKKYKGDIKVAEGFGKIGAVIVELQDGVN--ERFVD 79

Query: 96  TISITDKKSRNSTTFSCKSWVQSKSVLDQRRVFFST----------------------KK 155
           TIS+  +K   S TFSCKSWVQ K ++D RR+FFS+                      +K
Sbjct: 80  TISVEAEKPPISVTFSCKSWVQPKGLIDHRRIFFSSNKCRIYINGCVWPYNWDLDPASEK 139

Query: 156 RSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHK 215
           R +E FYVPRDEEFSE+KQ    SS+P N+ LL K  FSDLP +E      IK P A  K
Sbjct: 140 RIEEWFYVPRDEEFSEIKQS---SSQPGNEKLLRKSPFSDLPELE------IKTPSAASK 199

Query: 216 L-LTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGL 275
             L FN+++I++  +P    +S++   S+L+  PPPESY+RD+Y+WLSD EFAR TL GL
Sbjct: 200 FNLQFNIASILSSHRPPALKSSDTP-SSTLVELPPPESYKRDKYNWLSDIEFARLTLVGL 259

Query: 276 NPYSIQLVTRLPLMSELDPDTYGPQESAF 282
           NPYSIQLV  LP MS+LD   Y P+ES F
Sbjct: 260 NPYSIQLVKSLPFMSKLDEGDYRPRESKF 275

[Close]
BLAST of Csa4G287530 vs. TrEMBL
Match: A0A0A0L1U9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G286940 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.2e-55
Identity = 128/264 (48.48%), Postives = 175/264 (66.29%), Query Frame = 1 

Query: 1   MGVEVIASVTVKPKTKGPTYPYAN-IILKFASIELDSDQQQKPFIKCVVEPILPDDETEE 60
           M   +IA V+V PK+ G    Y + ++L FAS +LD D QQKP I+ V E ++ ++ T+ 
Sbjct: 1   MASNIIAIVSVIPKSNGSIIHYNDSLLLFFASSDLDRDGQQKPLIRGVAE-LIKNESTKA 60

Query: 61  YKKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSK 120
             KYV +V+VP+GYGEIGAV+VELE  T   EKFIDT+ + +  S+N+ TFSC SWVQ K
Sbjct: 61  KHKYVAKVQVPKGYGEIGAVVVELE--THSSEKFIDTVIVANSTSQNTITFSCTSWVQPK 120

Query: 121 SVLDQRRVFFSTKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIES 180
           +          +++RS + FYVPRDE FSEVKQ  FPS+   NKD LG   F+D   I+ 
Sbjct: 121 NP--------KSEQRSAKSFYVPRDEAFSEVKQTMFPSNS-GNKDKLGTKPFTDFTQIDL 180

Query: 181 MFREGIKAPHAPHKLLTFN-LSTIVTPDKPDLHSNSNSSVPSS--LLHYPPPESYRRDRY 240
           MFR+GI  P  PH++L FN LST+   ++P         V  +  ++ +PPPE+ +RD++
Sbjct: 181 MFRDGIALPSNPHEILQFNILSTLNASNQPAPPPVQPIPVQPNTPVIKFPPPEALKRDKF 240

Query: 241 SWLSDTEFARQTLAGLNPYSIQLV 261
           +WLSD+EFARQT+AGLNPY IQLV
Sbjct: 241 NWLSDSEFARQTIAGLNPYCIQLV 252

[Close]
BLAST of Csa4G287530 vs. TAIR10
Match: AT3G45140.1 (AT3G45140.1 lipoxygenase 2)

HSP 1 Score: 97.1 bits (240), Expect = 2.4e-20
Identity = 67/180 (37.22%), Postives = 88/180 (48.89%), Query Frame = 1 

Query: 130 STKKRSKERFYVPRDEEFSEVKQHYF---------PSSEPDNKDLL--GKDSFSDLPHIE 189
           S+++R    FYVPRDEEFS  K   F         PS  P  + +L   ++ F     I+
Sbjct: 286 SSEQRYGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQ 345

Query: 190 SMFREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSW 249
           ++F EGI+ P     L          P  P +   +       +L +  P    RDR+SW
Sbjct: 346 NLFEEGIQLPKDAGLL----------PLLPRI-IKALGEAQDDILQFDAPVLINRDRFSW 405

Query: 250 LSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE 299
           L D EFARQTLAGLNPYSIQLV   PL+S+LDP  YG   S      V+  +  ++ V+E
Sbjct: 406 LRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDE 454


HSP 2 Score: 50.4 bits (119), Expect = 2.6e-06
Identity = 31/110 (28.18%), Postives = 54/110 (49.09%), Query Frame = 1 

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           +KY  E E+PE +G +GA+ ++      +Y + +    +  K    S TF+C+SWV  KS
Sbjct: 135 EKYECEFEMPEDFGPVGAIKIQ-----NQYHRQLFLKGVELKLPGGSITFTCESWVAPKS 194

Query: 121 VLDQRRVFFSTKKRSKERFYVPRD--EEFSEVKQHYFPSSEPDNKDLLGK 169
           V   +R+FFS K       Y+P    E   + ++    + +  N++ +G+
Sbjct: 195 VDPTKRIFFSDKS------YLPSQTPEPLKKYRKEELETLQGKNREEVGE 233

[Close]
BLAST of Csa4G287530 vs. TAIR10
Match: AT1G72520.1 (AT1G72520.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 89.4 bits (220), Expect = 5.0e-18
Identity = 55/148 (37.16%), Postives = 76/148 (51.35%), Query Frame = 1 

Query: 140 YVPRDEEFSEVKQHYFPSSE---------PDNKDLLGKDSFSDLPHIESMFREGI--KAP 199
           YVPRDE+F E KQ+ F +           P  K  +  + F++   I+S+++EG+  K  
Sbjct: 324 YVPRDEQFEESKQNTFAACRLKAVLHNLIPSLKASILAEDFANFGEIDSLYKEGLLLKLG 383

Query: 200 HAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQT 259
                   F L  IVT     L  +S       LL Y  P+   +D+Y+WL D EFARQ 
Sbjct: 384 FQDDMFKKFPLPKIVTT----LQKSSEG-----LLRYDTPKIVSKDKYAWLRDDEFARQA 443

Query: 260 LAGLNPYSIQLVTRLPLMSELDPDTYGP 277
           +AG+NP +I+ VT  P +S LDP+ YGP
Sbjct: 444 IAGINPVNIERVTSYPPVSNLDPEIYGP 462

[Close]
BLAST of Csa4G287530 vs. TAIR10
Match: AT1G67560.1 (AT1G67560.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 87.0 bits (214), Expect = 2.5e-17
Identity = 60/180 (33.33%), Postives = 86/180 (47.78%), Query Frame = 1 

Query: 131 TKKRSKERFYVPRDEEFSEVKQHYF-------------PS--SEPDNKDLLGKDSFSDLP 190
           ++ + KE FYVPRDE F E+K+  F             PS  +   N D+     F+   
Sbjct: 302 SRGKEKEEFYVPRDEVFEEIKRDTFRAGRFKALFHNLVPSIAAALSNLDI----PFTCFS 361

Query: 191 HIESMFREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDR 250
            I+++++  I   H   K     L   +         N   +V  +LL Y  P   + DR
Sbjct: 362 DIDNLYKSNIVLGHTEPK--DTGLGGFIGG-----FMNGILNVTETLLKYDTPAVIKWDR 421

Query: 251 YSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIE 296
           ++WL D EF RQ LAG+NP +I+L+  LP+ S LDP  YGPQES       +E+I   +E
Sbjct: 422 FAWLRDNEFGRQALAGVNPVNIELLKELPIRSNLDPALYGPQESVL----TEEIIAREVE 466

[Close]
BLAST of Csa4G287530 vs. TAIR10
Match: AT1G17420.1 (AT1G17420.1 lipoxygenase 3)

HSP 1 Score: 84.7 bits (208), Expect = 1.2e-16
Identity = 51/150 (34.00%), Postives = 71/150 (47.33%), Query Frame = 1 

Query: 140 YVPRDEEFSEVKQHYFPSSE---------PDNKDLLGKDSFSDLPHIESMFREGIKAPHA 199
           YVPRDE+F E KQ  F +           P  K  +  + F+D   I+ +++EG+     
Sbjct: 318 YVPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKASIVAEDFADFGEIDRLYKEGLLLK-- 377

Query: 200 PHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLA 259
               L F          P +  ++       LL Y  P+   +D+ +WL D EFARQ +A
Sbjct: 378 ----LGFQDDIFKKFPLPKVVVDTLQESTKGLLKYDTPKILSKDKNAWLRDDEFARQAIA 437

Query: 260 GLNPYSIQLVTRLPLMSELDPDTYGPQESA 281
           G+NP +I+ V   P +S LDP  YGPQ SA
Sbjct: 438 GINPVNIERVKTFPPVSNLDPKIYGPQHSA 461

[Close]
BLAST of Csa4G287530 vs. TAIR10
Match: AT1G55020.1 (AT1G55020.1 lipoxygenase 1)

HSP 1 Score: 69.7 bits (169), Expect = 4.1e-12
Identity = 53/171 (30.99%), Postives = 78/171 (45.61%), Query Frame = 1 

Query: 140 YVPRDEEFSEVKQHYF-------------PSSEPDNKDLLGK-DSFSDLPHIESMFREGI 199
           YVPRDE F  +K   F             P+ E    D   + DSF D+  I   + EGI
Sbjct: 257 YVPRDERFGHLKMSDFLAYALKAIAQFIQPALEAVFDDTPKEFDSFEDVLKI---YEEGI 316

Query: 200 KAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFA 259
             P+         + +IV     ++      +     L +P P+  + D+ +W +D EFA
Sbjct: 317 DLPNQAL------IDSIVKNIPLEMLKEIFRTDGQKFLKFPVPQVIKEDKTAWRTDEEFA 376

Query: 260 RQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQ-ELIGCSIE 296
           R+ LAGLNP  IQL+   P  S+LD ++YG Q S    + ++  L G ++E
Sbjct: 377 REMLAGLNPVVIQLLKEFPPKSKLDSESYGNQNSTITKSHIEHNLDGLTVE 418

[Close]
BLAST of Csa4G287530 vs. NCBI nr
Match: gi|700198973|gb|KGN54131.1| (hypothetical protein Csa_4G287530 [Cucumis sativus])

HSP 1 Score: 724.9 bits (1870), Expect = 6.7e-206
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 1 

Query: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60
           MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY
Sbjct: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS
Sbjct: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120

Query: 121 VLDQRRVFFSTKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESM 180
           VLDQRRVFFSTKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESM
Sbjct: 121 VLDQRRVFFSTKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESM 180

Query: 181 FREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLS 240
           FREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLS
Sbjct: 181 FREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLS 240

Query: 241 DTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEWM 300
           DTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEWM
Sbjct: 241 DTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEWM 300

Query: 301 VIPSGNKFLHQALRPQICGYGELPKLMSLLMILVFTNLLSTGTYVIYPYIHGKKKK 357
           VIPSGNKFLHQALRPQICGYGELPKLMSLLMILVFTNLLSTGTYVIYPYIHGKKKK
Sbjct: 301 VIPSGNKFLHQALRPQICGYGELPKLMSLLMILVFTNLLSTGTYVIYPYIHGKKKK 356

[Close]
BLAST of Csa4G287530 vs. NCBI nr
Match: gi|449448968|ref|XP_004142237.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 345.1 bits (884), Expect = 1.4e-91
Identity = 166/168 (98.81%), Postives = 168/168 (100.00%), Query Frame = 1 

Query: 131 TKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHA 190
           ++KRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHA
Sbjct: 221 SEKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHA 280

Query: 191 PHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLA 250
           PHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLA
Sbjct: 281 PHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLA 340

Query: 251 GLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE 299
           GLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE
Sbjct: 341 GLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE 388

[Close]
BLAST of Csa4G287530 vs. NCBI nr
Match: gi|449448968|ref|XP_004142237.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 267.7 bits (683), Expect = 2.9e-68
Identity = 134/138 (97.10%), Postives = 134/138 (97.10%), Query Frame = 1 

Query: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60
           MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY
Sbjct: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS
Sbjct: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120

Query: 121 VLDQRRVFFSTKKRSKER 139
           VLDQRRVFFSTK    ER
Sbjct: 121 VLDQRRVFFSTKSYLPER 138


HSP 2 Score: 312.4 bits (799), Expect = 1.0e-81
Identity = 155/169 (91.72%), Postives = 160/169 (94.67%), Query Frame = 1 

Query: 131 TKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHA 190
           ++KRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGK+SF DLPHIESMFREGIKAP A
Sbjct: 221 SEKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKNSFHDLPHIESMFREGIKAPDA 280

Query: 191 PHKLLTFNLSTIVTPD-KPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTL 250
           PHKLL FNLSTIVTPD KPDLHSNS  SVPSSLLHYPPPESYRRDRYSWLSDTEFARQTL
Sbjct: 281 PHKLLKFNLSTIVTPDHKPDLHSNS--SVPSSLLHYPPPESYRRDRYSWLSDTEFARQTL 340

Query: 251 AGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE 299
           AGLNPYSIQLVTRLPLMSELDP+ YGPQESAFNHTKVQEL+GC  EVNE
Sbjct: 341 AGLNPYSIQLVTRLPLMSELDPEIYGPQESAFNHTKVQELLGCFNEVNE 387

[Close]
BLAST of Csa4G287530 vs. NCBI nr
Match: gi|659097677|ref|XP_008449754.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 239.6 bits (610), Expect = 8.5e-60
Identity = 118/132 (89.39%), Postives = 123/132 (93.18%), Query Frame = 1 

Query: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60
           MGVE   SVTVKPKT GPTYPYANIILKFASIELDSDQQQK FIKCVVEPI PDD+TEEY
Sbjct: 1   MGVEATVSVTVKPKTTGPTYPYANIILKFASIELDSDQQQKQFIKCVVEPIRPDDDTEEY 60

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           K+Y GEVEVPEGYGEIGAVIVELE DT KYEKFIDTIS+TDKKSR+STTFSCKSWVQSKS
Sbjct: 61  KEYEGEVEVPEGYGEIGAVIVELECDTAKYEKFIDTISLTDKKSRHSTTFSCKSWVQSKS 120

Query: 121 VLDQRRVFFSTK 133
           VLDQRR+FFSTK
Sbjct: 121 VLDQRRIFFSTK 132


HSP 2 Score: 277.7 bits (709), Expect = 2.8e-71
Identity = 163/315 (51.75%), Postives = 209/315 (66.35%), Query Frame = 1 

Query: 1   MGVEVIASVTVKPKTKGPTYPYAN-IILKFASIELDSDQQQKPFIKCVVEPILPDDETEE 60
           M   +IA V+V PK    T+P  + ++L FAS +LD D QQKP I  V E I  +   E+
Sbjct: 1   MASNIIAIVSVIPKIN-ETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEK 60

Query: 61  YKKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSK 120
           YK YV +++VP+G+GEIGAVIVEL+ D++  EKFIDT+ + +  S N+ TFSC SWVQ K
Sbjct: 61  YK-YVAKIQVPKGFGEIGAVIVELKDDSS--EKFIDTVIVANPTSHNTITFSCTSWVQPK 120

Query: 121 SVL-DQRRVFFSTK------------KRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLL 180
           S++ DQRRVFFSTK            +RS E FYVPRDE FSEVKQ  FPS+ P  KD L
Sbjct: 121 SLIPDQRRVFFSTKSNIEIDFDPKSEQRSTESFYVPRDEAFSEVKQKMFPSN-PGKKDKL 180

Query: 181 GKDSFSDLPHIESMFREGIKAPHAPHKLLTFNL-STIVTPDKPDLHSNSNSSVPSS--LL 240
           G   F+D   I+ MFR+GI  P  PH++L FN+ ST+   ++P         V  +  ++
Sbjct: 181 GAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPPVQPIPVQPNTPVI 240

Query: 241 HYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNH 299
            +PPPE+ +RD+++WLSD+EFARQT+AGLNPY IQLV   PL SELDP  YGPQESAF  
Sbjct: 241 KFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELDPVEYGPQESAFTT 300

[Close]
BLAST of Csa4G287530 vs. NCBI nr
Match: gi|700198971|gb|KGN54129.1| (hypothetical protein Csa_4G287020 [Cucumis sativus])

HSP 1 Score: 257.7 bits (657), Expect = 3.0e-65
Identity = 168/353 (47.59%), Postives = 210/353 (59.49%), Query Frame = 1 

Query: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60
           M  +   +VTV PK          I L+F S+ELDS   Q+  I C        D +   
Sbjct: 1   MSYKGTVTVTVNPKKNEAFLTIEVIYLEFVSVELDSKGNQQ-IIGCTAGLKCGGDVS--- 60

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           K +   ++VP  +GEIGAVIVEL+ D    E+FIDTI+I  KK     TFSCKSWVQ K+
Sbjct: 61  KLFETSIDVPGDFGEIGAVIVELDDDVK--ERFIDTITIDFKKC-TLLTFSCKSWVQPKN 120

Query: 121 VLDQRRVFFSTKKRSKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESM 180
              + R+        +  FYVPRDE FSE KQ    S   +N+ LLGK  FSDLP ++  
Sbjct: 121 KHAEERI--------EAPFYVPRDENFSEEKQG---SVLAENEALLGKAPFSDLPELD-- 180

Query: 181 FREGIKAPHAPHKL--LTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSW 240
               IK P A  K+  L F++ ++V+ ++P    NS    PSS +  P PESY+RD+Y+W
Sbjct: 181 ----IKTPFAASKINQLPFDIPSLVSSNQPP-PLNSLDDEPSSSVELPLPESYKRDKYNW 240

Query: 241 LSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNE 300
           LSD EFAR TLAGLNPYSIQLV  LP MS+LD   YGP+ES F H  VQEL+GC I+V +
Sbjct: 241 LSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGDYGPRESKFTHELVQELLGCRIKVCK 300

Query: 301 WMVIPSGNKFLHQALRPQICGYGELPKLMSLLMILVFTNLLSTGTYVIYPYIH 352
             ++ +G KF HQ L   ICGYG L K MS L IL F NLL TG Y I+ YIH
Sbjct: 301 -DILNNGKKFSHQVLIQPICGYGGLRKPMSFLTILAFINLLFTGMY-IHIYIH 326

The following BLAST results are available for this feature:
BLAST of Csa4G287530 vs. Swiss-Prot
Match NameE-valueIdentityDescription
LOX23_HORVU7.0e-2235.86Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1[more]
LOXC1_ORYSJ2.5e-1935.03Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV... [more]
LOX21_SOLTU4.3e-1933.19Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=... [more]
LOX2_ARATH4.3e-1937.22Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1[more]
LOXC2_ORYSJ1.6e-1836.72Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX... [more]
BLAST of Csa4G287530 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L1V5_CUCSA4.7e-206100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G287530 PE=4 SV=1[more]
A0A0A0KX68_CUCSA2.0e-7151.75Uncharacterized protein OS=Cucumis sativus GN=Csa_4G286960 PE=4 SV=1[more]
A0A0A0KZ89_CUCSA2.1e-6547.59Uncharacterized protein OS=Cucumis sativus GN=Csa_4G287020 PE=4 SV=1[more]
A0A0A0KX73_CUCSA3.3e-5849.44Uncharacterized protein OS=Cucumis sativus GN=Csa_4G287550 PE=4 SV=1[more]
A0A0A0L1U9_CUCSA1.2e-5548.48Uncharacterized protein OS=Cucumis sativus GN=Csa_4G286940 PE=4 SV=1[more]
BLAST of Csa4G287530 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G45140.12.4e-2037.22 lipoxygenase 2[more]
AT1G72520.15.0e-1837.16 PLAT/LH2 domain-containing lipoxygenase family protein[more]
AT1G67560.12.5e-1733.33 PLAT/LH2 domain-containing lipoxygenase family protein[more]
AT1G17420.11.2e-1634.00 lipoxygenase 3[more]
AT1G55020.14.1e-1230.99 lipoxygenase 1[more]
BLAST of Csa4G287530 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700198973|gb|KGN54131.1|6.7e-206100.00hypothetical protein Csa_4G287530 [Cucumis sativus][more]
gi|449448968|ref|XP_004142237.1|1.4e-9198.81PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus][more]
gi|449448968|ref|XP_004142237.1|2.9e-6897.10PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus][more]
gi|659097677|ref|XP_008449754.1|8.5e-6089.39PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis melo][more]
gi|700198971|gb|KGN54129.1|3.0e-6547.59hypothetical protein Csa_4G287020 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000907LipOase
IPR001024PLAT/LH2_dom
IPR013819LipOase_C
IPR027433Lipoxygenase_dom_3
Vocabulary: Molecular Function
TermDefinition
GO:0016702oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0046872metal ion binding
GO:0005515protein binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function GO:0005515 protein binding
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU116984cucumber EST collection version 3.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa4G287530.1Csa4G287530.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU116984CU116984transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000907LipoxygenasePANTHERPTHR11771LIPOXYGENASEcoord: 25..178
score: 7.0E-32coord: 220..300
score: 7.0
IPR001024PLAT/LH2 domainGENE3DG3DSA:2.60.60.20coord: 24..154
score: 2.8
IPR001024PLAT/LH2 domainSMARTSM00308LH2_4coord: 3..131
score: 0.
IPR001024PLAT/LH2 domainPROFILEPS50095PLATcoord: 5..130
score: 17
IPR001024PLAT/LH2 domainunknownSSF49723Lipase/lipooxygenase domain (PLAT/LH2 domain)coord: 24..132
score: 1.18
IPR013819Lipoxygenase, C-terminalPFAMPF00305Lipoxygenasecoord: 131..295
score: 3.4
IPR013819Lipoxygenase, C-terminalPROFILEPS51393LIPOXYGENASE_3coord: 50..356
score: 18
IPR013819Lipoxygenase, C-terminalunknownSSF48484Lipoxigenasecoord: 135..286
score: 2.35
IPR027433Lipoxygenase, domain 3GENE3DG3DSA:4.10.372.10coord: 168..254
score: 2.5
NoneNo IPR availablePANTHERPTHR11771:SF42PROTEIN C13C4.7coord: 220..300
score: 7.0E-32coord: 25..178
score: 7.0

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa4G287530Cucumber (Gy14) v2cgybcuB167
Csa4G287530Melon (DHL92) v3.6.1cumedB279
Csa4G287530Silver-seed gourdcarcuB0552
Csa4G287530Watermelon (97103) v2cuwmbB287
Csa4G287530Wax gourdcuwgoB390
Csa4G287530Cucurbita maxima (Rimu)cmacuB549
Csa4G287530Cucurbita moschata (Rifu)cmocuB538
Csa4G287530Cucurbita moschata (Rifu)cmocuB596
Csa4G287530Melon (DHL92) v3.5.1cumeB295
Csa4G287530Watermelon (Charleston Gray)cuwcgB292
Csa4G287530Watermelon (97103) v1cuwmB347
Csa4G287530Bottle gourd (USVL1VR-Ls)culsiB246