Csa4G286360 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa4G286360
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionFatty acid desaturase; contains IPR005804 (Fatty acid desaturase, type 1), IPR021863 (Protein of unknown function DUF3474)
LocationChr4 : 11058268 .. 11058771 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAAGGAAGCCCAAATAGAAGCAAATTAGCATTGGGGGAGCGCATTACACACACCAAGCCTCCCTTCACCATCACACAAATCAAGAAATCCATTCCATCCCATTGCTTCAATCGTTCCCTCTACCGCTCCTTCTCTTACGTTATCTTCGACTCCATCCTTCTCTCTACTTTTTATTACGTAGCCACCACCTATTTCCACACCCTTTCCGCCCACCCACTACTGCTTCACTACTTAGCTTGGCCTCTTTATTGGCTCTCTCAAGGCATTGTCTTCACCGGCTTCTGGGTCATCGCTCATGAATGTGGCCATCATGCCTTCAGTGATTACCAATTCCTAGATGACTTCCTTGGCTTCCTCCTCCATTCTTCCCTCCTCATCCCTTACTTCTCTTTCAAAATCAGCCACCGCCGCCACCATGCCAACACTGCATCACTCCACCGCGACGAGGTCTTTGTCCCCAAGCCCAAATCCAAAATCCCTTGGTATGGTGTTAATTAG

mRNA sequence

ATGAGGAAAGGAAGCCCAAATAGAAGCAAATTAGCATTGGGGGAGCGCATTACACACACCAAGCCTCCCTTCACCATCACACAAATCAAGAAATCCATTCCATCCCATTGCTTCAATCGTTCCCTCTACCGCTCCTTCTCTTACGTTATCTTCGACTCCATCCTTCTCTCTACTTTTTATTACGTAGCCACCACCTATTTCCACACCCTTTCCGCCCACCCACTACTGCTTCACTACTTAGCTTGGCCTCTTTATTGGCTCTCTCAAGGCATTGTCTTCACCGGCTTCTGGGTCATCGCTCATGAATGTGGCCATCATGCCTTCAGTGATTACCAATTCCTAGATGACTTCCTTGGCTTCCTCCTCCATTCTTCCCTCCTCATCCCTTACTTCTCTTTCAAAATCAGCCACCGCCGCCACCATGCCAACACTGCATCACTCCACCGCGACGAGGTCTTTGTCCCCAAGCCCAAATCCAAAATCCCTTGGTATGGTGTTAATTAG

Coding sequence (CDS)

ATGAGGAAAGGAAGCCCAAATAGAAGCAAATTAGCATTGGGGGAGCGCATTACACACACCAAGCCTCCCTTCACCATCACACAAATCAAGAAATCCATTCCATCCCATTGCTTCAATCGTTCCCTCTACCGCTCCTTCTCTTACGTTATCTTCGACTCCATCCTTCTCTCTACTTTTTATTACGTAGCCACCACCTATTTCCACACCCTTTCCGCCCACCCACTACTGCTTCACTACTTAGCTTGGCCTCTTTATTGGCTCTCTCAAGGCATTGTCTTCACCGGCTTCTGGGTCATCGCTCATGAATGTGGCCATCATGCCTTCAGTGATTACCAATTCCTAGATGACTTCCTTGGCTTCCTCCTCCATTCTTCCCTCCTCATCCCTTACTTCTCTTTCAAAATCAGCCACCGCCGCCACCATGCCAACACTGCATCACTCCACCGCGACGAGGTCTTTGTCCCCAAGCCCAAATCCAAAATCCCTTGGTATGGTGTTAATTAG

Protein sequence

MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYGVN*
Homology
[Close]
BLAST of Csa4G286360 vs. Swiss-Prot
Match: FADX_TRIKI (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAC PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 2.8e-69
Identity = 124/164 (75.61%), Postives = 136/164 (82.93%), Query Frame = 1 

Query: 1   MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60
           +R GS + +KLA GERITH KPPF+I+QIKK+IP HCF RSLYRSFSYVIFD I  STFY
Sbjct: 9   VRSGSSS-TKLAFGERITHAKPPFSISQIKKAIPPHCFQRSLYRSFSYVIFDFIFASTFY 68

Query: 61  YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF 120
           ++A T FH L  HPL  HYLAWPLYW  QG VFTG WVIAHECGH AFSDYQ +DD +GF
Sbjct: 69  HIAATNFHRLP-HPL--HYLAWPLYWFCQGSVFTGLWVIAHECGHRAFSDYQLVDDVVGF 128

Query: 121 LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
           LLH+S LIPYFSFKISHRRHH+NTASL RDEVFVPKPK+K+PWY
Sbjct: 129 LLHTSFLIPYFSFKISHRRHHSNTASLERDEVFVPKPKAKMPWY 168

[Close]
BLAST of Csa4G286360 vs. Swiss-Prot
Match: FADX_MOMCH (Delta(12) acyl-lipid conjugase (11E,13E-forming) OS=Momordica charantia GN=FADX PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 3.9e-63
Identity = 109/154 (70.78%), Postives = 126/154 (81.82%), Query Frame = 1 

Query: 11  LALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTL 70
           L  GERITH +PPF+I+QIKK+IP HCF RSL RSFSY++ D  L+S FYYVA TYFH L
Sbjct: 26  LGPGERITHARPPFSISQIKKAIPPHCFQRSLRRSFSYLLSDIALVSAFYYVADTYFHRL 85

Query: 71  SAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPY 130
             HPLL HYLAWP+YW  QG V TG W IAH+CGHHAFSDYQ +DD +GFL+HS + +PY
Sbjct: 86  P-HPLL-HYLAWPVYWFCQGAVLTGMWGIAHDCGHHAFSDYQLVDDVVGFLIHSLVFVPY 145

Query: 131 FSFKISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
           FSFKISHRRHH+NT+S+ RDEVFVPKPK+K+PWY
Sbjct: 146 FSFKISHRRHHSNTSSVDRDEVFVPKPKAKMPWY 177

[Close]
BLAST of Csa4G286360 vs. Swiss-Prot
Match: FADX_VERFO (Bifunctional desaturase/conjugase FADX OS=Vernicia fordii PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 1.4e-57
Identity = 103/165 (62.42%), Postives = 122/165 (73.94%), Query Frame = 1 

Query: 5   SPNRSKLALGE-----RITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTF 64
           +PN SK    E     R+ HTKPPFT+ Q+K++IPSHCF RSL RSFSYV++D  L   F
Sbjct: 10  APNNSKCEKKESRSVKRVPHTKPPFTLGQLKQAIPSHCFKRSLLRSFSYVVYDLSLSFIF 69

Query: 65  YYVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLG 124
           Y +ATTYFH L   P  + Y+AWP+YW  QG + T  WV+ HECGHHAFS+Y +LDD +G
Sbjct: 70  YSIATTYFHLL---PSPITYIAWPVYWAFQGCILTSVWVLGHECGHHAFSEYNWLDDTIG 129

Query: 125 FLLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
            +LHSSLL+PYFSFKISHRRHH+N ASL RDEVFVP+ KS IPWY
Sbjct: 130 LILHSSLLVPYFSFKISHRRHHSNIASLERDEVFVPRLKSAIPWY 171

[Close]
BLAST of Csa4G286360 vs. Swiss-Prot
Match: FAD12_PUNGR (Delta(12)-acyl-lipid-desaturase OS=Punica granatum GN=FAD12 PE=2 SV=2)

HSP 1 Score: 223.4 bits (568), Expect = 1.9e-57
Identity = 99/150 (66.00%), Postives = 119/150 (79.33%), Query Frame = 1 

Query: 15  ERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTLSAHP 74
           +R+   KPPFT+++IKK+IP HCF RSL +SFSYV++D  L++ FYYVATTY   L   P
Sbjct: 26  QRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFSYVLYDLTLVAIFYYVATTYIDAL---P 85

Query: 75  LLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPYFSFK 134
             L Y AWP+YW  QG V TG WVIAHECGHHAFSDYQ++DD +G +LHS+LL+PYFS+K
Sbjct: 86  GPLRYAAWPVYWALQGCVLTGVWVIAHECGHHAFSDYQWVDDCVGLVLHSALLVPYFSWK 145

Query: 135 ISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
            SHRRHH+NT SL RDEVFVPKPKSK+PW+
Sbjct: 146 YSHRRHHSNTGSLERDEVFVPKPKSKMPWF 172

[Close]
BLAST of Csa4G286360 vs. Swiss-Prot
Match: FAD6E_BRAJU (Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 2.4e-57
Identity = 104/161 (64.60%), Postives = 122/161 (75.78%), Query Frame = 1 

Query: 5   SPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVAT 64
           SP +S+    +R+    PPFT+ ++KK+IP HCF RS+ RSFSY+I+D I+ S FYYVAT
Sbjct: 12  SPKKSETDTLKRVPCETPPFTVGELKKAIPPHCFKRSIPRSFSYLIWDIIVASCFYYVAT 71

Query: 65  TYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHS 124
           TYF  L  HPL   Y+AWPLYW  QG+V TG WVIAHECGHHAFSDYQ+LDD +G + HS
Sbjct: 72  TYFPLLP-HPL--SYVAWPLYWACQGVVLTGVWVIAHECGHHAFSDYQWLDDTVGLIFHS 131

Query: 125 SLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYG 166
            LL+PYFS+K SHRRHH+NT SL RDEVFVPK KS I WYG
Sbjct: 132 FLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSDIKWYG 169

[Close]
BLAST of Csa4G286360 vs. TrEMBL
Match: A0A0A0L1U1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G286360 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 1.6e-95
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 1 

Query: 1   MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60
           MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY
Sbjct: 1   MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60

Query: 61  YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF 120
           YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF
Sbjct: 61  YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF 120

Query: 121 LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYGVN 168
           LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYGVN
Sbjct: 121 LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYGVN 167

[Close]
BLAST of Csa4G286360 vs. TrEMBL
Match: R9W7B9_9ROSI (Fatty acid delta12-desturase FAD2-3 OS=Hiptage benghalensis PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.2e-58
Identity = 104/150 (69.33%), Postives = 123/150 (82.00%), Query Frame = 1 

Query: 15  ERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTLSAHP 74
           +R+ H KPPFT+ ++KK+IPSHCF RSL+RSFSYVI D  L S FYY+A+ YFH L   P
Sbjct: 24  KRVPHAKPPFTLGELKKAIPSHCFQRSLFRSFSYVIHDLFLSSVFYYIASNYFHLL---P 83

Query: 75  LLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPYFSFK 134
             L Y+AWP+YW+ QG V TG WVIAHECGHHAFSDYQ +DD +G +LHS+LL+PYFS+K
Sbjct: 84  SPLCYIAWPVYWVFQGCVLTGVWVIAHECGHHAFSDYQLVDDIVGLILHSALLVPYFSWK 143

Query: 135 ISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
           ISHRRHH+NT SL RDEVFVPKPKSKI WY
Sbjct: 144 ISHRRHHSNTGSLDRDEVFVPKPKSKIAWY 170

[Close]
BLAST of Csa4G286360 vs. TrEMBL
Match: F6GUE8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g01250 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 7.6e-58
Identity = 105/150 (70.00%), Postives = 121/150 (80.67%), Query Frame = 1 

Query: 15  ERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTLSAHP 74
           +R+ HTKPPFT+ QIKK+IP HCF RS+ RSFSYV +D  L   FYY+AT+YF  L   P
Sbjct: 59  QRVPHTKPPFTLGQIKKAIPPHCFQRSIPRSFSYVAYDLSLAFLFYYIATSYFSLL---P 118

Query: 75  LLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPYFSFK 134
             L YLAWPLYW  QG   TG WVIAHECGHHAFSDYQ+LDD +G +LHSSLL+PYFS+K
Sbjct: 119 SWLTYLAWPLYWTLQGCTLTGVWVIAHECGHHAFSDYQWLDDTVGLILHSSLLVPYFSWK 178

Query: 135 ISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
           ISHRRHH+NT SL RDEVFVPKPKS+IPW+
Sbjct: 179 ISHRRHHSNTGSLERDEVFVPKPKSRIPWF 205

[Close]
BLAST of Csa4G286360 vs. TrEMBL
Match: A5ATS6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_002517 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 7.6e-58
Identity = 105/150 (70.00%), Postives = 121/150 (80.67%), Query Frame = 1 

Query: 15  ERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTLSAHP 74
           +R+ HTKPPFT+ QIKK+IP HCF RS+ RSFSYV +D  L   FYY+AT+YF  L   P
Sbjct: 15  QRVPHTKPPFTLGQIKKAIPPHCFQRSIPRSFSYVAYDLSLAFLFYYIATSYFSLL---P 74

Query: 75  LLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPYFSFK 134
             L YLAWPLYW  QG   TG WVIAHECGHHAFSDYQ+LDD +G +LHSSLL+PYFS+K
Sbjct: 75  SWLTYLAWPLYWTLQGCTLTGVWVIAHECGHHAFSDYQWLDDTVGLILHSSLLVPYFSWK 134

Query: 135 ISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
           ISHRRHH+NT SL RDEVFVPKPKS+IPW+
Sbjct: 135 ISHRRHHSNTGSLERDEVFVPKPKSRIPWF 161

[Close]
BLAST of Csa4G286360 vs. TrEMBL
Match: A5C4J4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_018075 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 7.6e-58
Identity = 105/150 (70.00%), Postives = 121/150 (80.67%), Query Frame = 1 

Query: 15  ERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTLSAHP 74
           +R+ HTKPPFT+ QIKK+IP HCF RS+ RSFSYV +D  L   FYY+AT+YF  L   P
Sbjct: 15  QRVPHTKPPFTLGQIKKAIPPHCFQRSIPRSFSYVAYDLSLAFLFYYIATSYFSLL---P 74

Query: 75  LLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPYFSFK 134
             L YLAWPLYW  QG   TG WVIAHECGHHAFSDYQ+LDD +G +LHSSLL+PYFS+K
Sbjct: 75  SWLTYLAWPLYWTLQGCTLTGVWVIAHECGHHAFSDYQWLDDTVGLILHSSLLVPYFSWK 134

Query: 135 ISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
           ISHRRHH+NT SL RDEVFVPKPKS+IPW+
Sbjct: 135 ISHRRHHSNTGSLERDEVFVPKPKSRIPWF 161

[Close]
BLAST of Csa4G286360 vs. TAIR10
Match: AT3G12120.1 (AT3G12120.1 fatty acid desaturase 2)

HSP 1 Score: 218.0 bits (554), Expect = 4.4e-57
Identity = 102/161 (63.35%), Postives = 117/161 (72.67%), Query Frame = 1 

Query: 5   SPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVAT 64
           S  +S+    +R+   KPPF++  +KK+IP HCF RS+ RSFSY+I D I+ S FYYVAT
Sbjct: 12  SSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDIIIASCFYYVAT 71

Query: 65  TYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHS 124
            YF  L   P  L YLAWPLYW  QG V TG WVIAHECGHHAFSDYQ+LDD +G + HS
Sbjct: 72  NYFSLL---PQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDDTVGLIFHS 131

Query: 125 SLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYG 166
            LL+PYFS+K SHRRHH+NT SL RDEVFVPK KS I WYG
Sbjct: 132 FLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYG 169

[Close]
BLAST of Csa4G286360 vs. TAIR10
Match: AT2G29980.1 (AT2G29980.1 fatty acid desaturase 3)

HSP 1 Score: 117.9 bits (294), Expect = 6.2e-27
Identity = 55/138 (39.86%), Postives = 80/138 (57.97%), Query Frame = 1 

Query: 21  KPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTLSAHPLLLHYL 80
           +PPF I  I+ +IP HC+ +S  RS SYV+ D I ++    +A  Y  +         + 
Sbjct: 31  QPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAALA-IAAVYVDS---------WF 90

Query: 81  AWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPYFSFKISHRRH 140
            WPLYW +QG +F   +V+ H+CGH +FSD   L+  +G +LHS +L+PY  ++ISHR H
Sbjct: 91  LWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTH 150

Query: 141 HANTASLHRDEVFVPKPK 159
           H N   +  DE +VP P+
Sbjct: 151 HQNHGHVENDESWVPLPE 158

[Close]
BLAST of Csa4G286360 vs. TAIR10
Match: AT5G05580.1 (AT5G05580.1 fatty acid desaturase 8)

HSP 1 Score: 115.2 bits (287), Expect = 4.0e-26
Identity = 55/138 (39.86%), Postives = 81/138 (58.70%), Query Frame = 1 

Query: 22  PPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTLSAHPLLLHYLA 81
           PPF +  I+ +IP HC+ ++ + S SYV+ D  ++     VA  YF+         ++L 
Sbjct: 87  PPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAA-YFN---------NWLL 146

Query: 82  WPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPYFSFKISHRRHH 141
           WPLYW +QG +F   +V+ H+CGH +FS+   L+   G LLHSS+L+PY  ++ISHR HH
Sbjct: 147 WPLYWFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHH 206

Query: 142 ANTASLHRDEVFVPKPKS 160
            N   +  DE + P P+S
Sbjct: 207 QNHGHVENDESWHPLPES 214

[Close]
BLAST of Csa4G286360 vs. TAIR10
Match: AT3G11170.1 (AT3G11170.1 fatty acid desaturase 7)

HSP 1 Score: 113.2 bits (282), Expect = 1.5e-25
Identity = 51/134 (38.06%), Postives = 79/134 (58.96%), Query Frame = 1 

Query: 22  PPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTLSAHPLLLHYLA 81
           PPF +  I+ +IP HC+ ++ ++S SYV+ D  ++          F   +    L +++ 
Sbjct: 94  PPFNLADIRAAIPKHCWVKNPWKSLSYVVRDVAIV----------FALAAGAAYLNNWIV 153

Query: 82  WPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPYFSFKISHRRHH 141
           WPLYWL+QG +F   +V+ H+CGH +FS+   L+  +G LLHSS+L+PY  ++ISHR HH
Sbjct: 154 WPLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHH 213

Query: 142 ANTASLHRDEVFVP 156
            N   +  DE + P
Sbjct: 214 QNHGHVENDESWHP 217

[Close]
BLAST of Csa4G286360 vs. NCBI nr
Match: gi|700198953|gb|KGN54111.1| (hypothetical protein Csa_4G286360 [Cucumis sativus])

HSP 1 Score: 356.7 bits (914), Expect = 2.2e-95
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 1 

Query: 1   MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60
           MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY
Sbjct: 1   MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60

Query: 61  YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF 120
           YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF
Sbjct: 61  YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF 120

Query: 121 LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYGVN 168
           LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYGVN
Sbjct: 121 LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYGVN 167

[Close]
BLAST of Csa4G286360 vs. NCBI nr
Match: gi|778693060|ref|XP_011653570.1| (PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [Cucumis sativus])

HSP 1 Score: 350.5 bits (898), Expect = 1.6e-93
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 1 

Query: 1   MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60
           MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY
Sbjct: 1   MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60

Query: 61  YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF 120
           YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF
Sbjct: 61  YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF 120

Query: 121 LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
           LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWY
Sbjct: 121 LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWY 164

[Close]
BLAST of Csa4G286360 vs. NCBI nr
Match: gi|659098044|ref|XP_008449949.1| (PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [Cucumis melo])

HSP 1 Score: 323.6 bits (828), Expect = 2.1e-85
Identity = 154/164 (93.90%), Postives = 157/164 (95.73%), Query Frame = 1 

Query: 1   MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60
           MRK SP+RSKLALGERITHTKPPFT TQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY
Sbjct: 1   MRKRSPSRSKLALGERITHTKPPFTFTQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60

Query: 61  YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF 120
           YVATTYFHTL  HPLLLHYLAWPLYWLSQGIVFTG WVIAHECGHHAFSDYQFLDD +GF
Sbjct: 61  YVATTYFHTLP-HPLLLHYLAWPLYWLSQGIVFTGLWVIAHECGHHAFSDYQFLDDLVGF 120

Query: 121 LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
           LLHSSLLIPYFSFKISHRRHHANTASL+RDEVFVPK KSKIPWY
Sbjct: 121 LLHSSLLIPYFSFKISHRRHHANTASLNRDEVFVPKLKSKIPWY 163

[Close]
BLAST of Csa4G286360 vs. NCBI nr
Match: gi|75328135|sp|Q84UC0.1|FADX_TRIKI (RecName: Full=Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase; Short=TkFAC; AltName: Full=Delta(12)-oleate desaturase; AltName: Full=Linoleoyl-lipid Delta(12) conjugase (11E,13Z-forming))

HSP 1 Score: 262.7 bits (670), Expect = 4.4e-67
Identity = 124/164 (75.61%), Postives = 138/164 (84.15%), Query Frame = 1 

Query: 1   MRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60
           +R GS + +KLA GERITH KPPF+I+QIKK+IP HCF RSLYRSFSYVIFD I  STFY
Sbjct: 9   VRSGSSS-TKLAFGERITHAKPPFSISQIKKAIPPHCFQRSLYRSFSYVIFDFIFASTFY 68

Query: 61  YVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGF 120
           ++A T FH L  HPL  HYLAWPLYW  QG VFTG WVIAHECGH AFSDYQ +DD +GF
Sbjct: 69  HIAATNFHRLP-HPL--HYLAWPLYWFCQGSVFTGLWVIAHECGHRAFSDYQLVDDVVGF 128

Query: 121 LLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
           LLH+S LIPYFSFKISHRRHH+NTASL RDEVFVPKPK+K+PWY
Sbjct: 129 LLHTSFLIPYFSFKISHRRHHSNTASLERDEVFVPKPKAKMPWY 168

[Close]
BLAST of Csa4G286360 vs. NCBI nr
Match: gi|75337482|sp|Q9SP61.1|FADX_MOMCH (RecName: Full=Delta(12) acyl-lipid conjugase (11E,13E-forming); Short=MomoFadX)

HSP 1 Score: 242.3 bits (617), Expect = 6.2e-61
Identity = 109/154 (70.78%), Postives = 128/154 (83.12%), Query Frame = 1 

Query: 11  LALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFYYVATTYFHTL 70
           L  GERITH +PPF+I+QIKK+IP HCF RSL RSFSY++ D  L+S FYYVA TYFH L
Sbjct: 26  LGPGERITHARPPFSISQIKKAIPPHCFQRSLRRSFSYLLSDIALVSAFYYVADTYFHRL 85

Query: 71  SAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDDFLGFLLHSSLLIPY 130
             HPLL HYLAWP+YW  QG V TG W IAH+CGHHAFSDYQ +DD +GFL+HS + +PY
Sbjct: 86  P-HPLL-HYLAWPVYWFCQGAVLTGMWGIAHDCGHHAFSDYQLVDDVVGFLIHSLVFVPY 145

Query: 131 FSFKISHRRHHANTASLHRDEVFVPKPKSKIPWY 165
           FSFKISHRRHH+NT+S+ RDEVFVPKPK+K+PWY
Sbjct: 146 FSFKISHRRHHSNTSSVDRDEVFVPKPKAKMPWY 177

The following BLAST results are available for this feature:
BLAST of Csa4G286360 vs. Swiss-Prot
Match NameE-valueIdentityDescription
FADX_TRIKI2.8e-6975.61Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes k... [more]
FADX_MOMCH3.9e-6370.78Delta(12) acyl-lipid conjugase (11E,13E-forming) OS=Momordica charantia GN=FADX ... [more]
FADX_VERFO1.4e-5762.42Bifunctional desaturase/conjugase FADX OS=Vernicia fordii PE=1 SV=1[more]
FAD12_PUNGR1.9e-5766.00Delta(12)-acyl-lipid-desaturase OS=Punica granatum GN=FAD12 PE=2 SV=2[more]
FAD6E_BRAJU2.4e-5764.60Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea PE=2 SV=... [more]
BLAST of Csa4G286360 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L1U1_CUCSA1.6e-95100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G286360 PE=4 SV=1[more]
R9W7B9_9ROSI1.2e-5869.33Fatty acid delta12-desturase FAD2-3 OS=Hiptage benghalensis PE=2 SV=1[more]
F6GUE8_VITVI7.6e-5870.00Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g01250 PE=4 SV=... [more]
A5ATS6_VITVI7.6e-5870.00Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_002517 PE=4 SV=1[more]
A5C4J4_VITVI7.6e-5870.00Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_018075 PE=4 SV=1[more]
BLAST of Csa4G286360 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G12120.14.4e-5763.35 fatty acid desaturase 2[more]
AT2G29980.16.2e-2739.86 fatty acid desaturase 3[more]
AT5G05580.14.0e-2639.86 fatty acid desaturase 8[more]
AT3G11170.11.5e-2538.06 fatty acid desaturase 7[more]
BLAST of Csa4G286360 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700198953|gb|KGN54111.1|2.2e-95100.00hypothetical protein Csa_4G286360 [Cucumis sativus][more]
gi|778693060|ref|XP_011653570.1|1.6e-93100.00PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [... [more]
gi|659098044|ref|XP_008449949.1|2.1e-8593.90PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [... [more]
gi|75328135|sp|Q84UC0.1|FADX_TRIKI4.4e-6775.61RecName: Full=Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase; Sho... [more]
gi|75337482|sp|Q9SP61.1|FADX_MOMCH6.2e-6170.78RecName: Full=Delta(12) acyl-lipid conjugase (11E,13E-forming); Short=MomoFadX[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005804FA_desaturase_dom
IPR021863FAS_N
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016717oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016720 delta12-fatty acid dehydrogenase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa4G286360.1Csa4G286360.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 81..160
score: 2.1
IPR021863Fatty acid desaturase, N-terminalPFAMPF11960DUF3474coord: 20..55
score: 3.
NoneNo IPR availablePANTHERPTHR32100FAMILY NOT NAMEDcoord: 15..162
score: 1.1
NoneNo IPR availablePANTHERPTHR32100:SF16SUBFAMILY NOT NAMEDcoord: 15..162
score: 1.1

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Csa4G286360CSPI04G13360Wild cucumber (PI 183967)cpicuB181
Csa4G286360Cp4.1LG05g05130Cucurbita pepo (Zucchini)cpecuB728
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa4G286360Cucurbita pepo (Zucchini)cpecuB278
Csa4G286360Bottle gourd (USVL1VR-Ls)culsiB246
Csa4G286360Cucumber (Gy14) v2cgybcuB167
Csa4G286360Melon (DHL92) v3.6.1cumedB279
Csa4G286360Silver-seed gourdcarcuB0552
Csa4G286360Silver-seed gourdcarcuB0935
Csa4G286360Watermelon (97103) v2cuwmbB287
Csa4G286360Wax gourdcuwgoB390
Csa4G286360Cucumber (Gy14) v1cgycuB220
Csa4G286360Cucumber (Gy14) v1cgycuB345
Csa4G286360Cucurbita maxima (Rimu)cmacuB549
Csa4G286360Cucurbita maxima (Rimu)cmacuB605
Csa4G286360Cucurbita moschata (Rifu)cmocuB538
Csa4G286360Cucurbita moschata (Rifu)cmocuB596
Csa4G286360Melon (DHL92) v3.5.1cumeB295
Csa4G286360Watermelon (Charleston Gray)cuwcgB292
Csa4G286360Watermelon (97103) v1cuwmB347