Csa4G189510 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa4G189510
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Subtilisin inhibitor; contains IPR000864 (Proteinase inhibitor I13, potato inhibitor I)
Location	Chr4 : 9386270 .. 9387075 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CATAACACAATCGCAATGTAGGCTAATTTACTTGGTTAATCAGAATGCCAAATAACAAAAACACGACATTTCAACCGTTCAATTGAAGTTGAAAATCCAGGCTTACTTTGAATTTGGCAAGGAGAGGGTTGCTCTGTATTGAAATGGAATTTCCATGAGTGCGGCCAAGGCAAATGTTGCTTCCTTCTTTGACGCTTCTGTGCTCTCAGACATGATCTTTGTGAAGTTTACAAACTGAATCAATAGAGAGAAGCCGTGAAGTTACACACTGAACCAAAAACAATTGAACACCCATGTTTTCTTTTTGAAAACCAGGAGATGGAGTTTTGCTCTCAGTGGTTTATGGATAACGTACAAACAGAAATTAGAAGGCATATTCCAAAAGAAAAAAAGAAAGAAAGTAGAATGAATGCTGACTATTATGATCGTGGTTCTCCAACTATGTGGCACCATGATCAATACCCATACCTGTTAATATCCATTTTTTTAAAACCCCTATAGCAGCAACTTTCTATAGAATATGTATTATTGTACTAAAAAAATGATTGCCACACAAATATTACACATAATCTTATAAGGAGCTAATGCAATTATTTCAACCGGAGACACCAAAGAATAAGGTATAGCCATCATTAGTGAGTTTGACAGCAGAAGAGGTAGAGAAGAAGATAAAAGAAGAAATGACAGAGGTTTATGTTTCAGTGGTTCCTCCTGATTATGCCATAACTTCTGATCTTAGATTTAATCGTGTTTGGCTATTCGTTGATTCTTCTGGCAAAGTTATACAAACACCTTCTATTGGTTAA

mRNA sequence

ATGAGTGCGGCCAAGGCAAATGTTGCTTCCTTCTTTGACGCTTCTGTGCTCTCAGACATGATCTTTGAGATGGAGTTTTGCTCTCAGTGGTTTATGGATAACGTACAAACAGAAATTAGAAGGCATATTCCAAAAGAAAAAAAGAAAGAAAGTAGAATGAATGCTGACTATTATGATCGTGCAGAAGAGGTAGAGAAGAAGATAAAAGAAGAAATGACAGAGGTTTATGTTTCAGTGGTTCCTCCTGATTATGCCATAACTTCTGATCTTAGATTTAATCGTGTTTGGCTATTCGTTGATTCTTCTGGCAAAGTTATACAAACACCTTCTATTGGTTAA

Coding sequence (CDS)

ATGAGTGCGGCCAAGGCAAATGTTGCTTCCTTCTTTGACGCTTCTGTGCTCTCAGACATGATCTTTGAGATGGAGTTTTGCTCTCAGTGGTTTATGGATAACGTACAAACAGAAATTAGAAGGCATATTCCAAAAGAAAAAAAGAAAGAAAGTAGAATGAATGCTGACTATTATGATCGTGCAGAAGAGGTAGAGAAGAAGATAAAAGAAGAAATGACAGAGGTTTATGTTTCAGTGGTTCCTCCTGATTATGCCATAACTTCTGATCTTAGATTTAATCGTGTTTGGCTATTCGTTGATTCTTCTGGCAAAGTTATACAAACACCTTCTATTGGTTAA

Protein sequence

MSAAKANVASFFDASVLSDMIFEMEFCSQWFMDNVQTEIRRHIPKEKKKESRMNADYYDRAEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG*

Homology

[Close]

BLAST of Csa4G189510 vs. Swiss-Prot
Match: ICI1_PHAAN (Subtilisin inhibitor 1 OS=Phaseolus angularis PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.5e-07
Identity = 28/52 (53.85%), Postives = 33/52 (63.46%), Query Frame = 1


Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AE+ E KIKEEM +V + V P D  +T+D    RV L+VD S KV +TPSIG
Sbjct: 41  AEQAETKIKEEMVDVQIQVSPHDSFVTADYNPKRVRLYVDESNKVTRTPSIG 92

[Close]

BLAST of Csa4G189510 vs. Swiss-Prot
Match: ICI1_CANLI (Subtilisin inhibitor CLSI-I OS=Canavalia lineata PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.7e-06
Identity = 29/52 (55.77%), Postives = 34/52 (65.38%), Query Frame = 1


Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AEE E KIKEEM+ V + VVPP   +T+D +  RV L+VD S KV +TP IG
Sbjct: 15  AEEAE-KIKEEMSGVEIQVVPPGSFVTADYKPQRVRLYVDESNKVTRTPGIG 65

[Close]

BLAST of Csa4G189510 vs. Swiss-Prot
Match: ICIS_VICFA (Subtilisin inhibitor (Fragment) OS=Vicia faba PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 3.8e-06
Identity = 25/50 (50.00%), Postives = 31/50 (62.00%), Query Frame = 1


Query: 63  EVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           E  +KIKEEM E  + VVP D  +T+D +F RV L+VD S KV++   IG
Sbjct: 13  EEARKIKEEMPEAEIQVVPQDSFVTADYKFQRVRLYVDESNKVVRAAPIG 62

[Close]

BLAST of Csa4G189510 vs. TrEMBL
Match: A0A0A0KWM2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G189510 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 1.6e-56
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 1


Query: 1   MSAAKANVASFFDASVLSDMIFEMEFCSQWFMDNVQTEIRRHIPKEKKKESRMNADYYDR 60
           MSAAKANVASFFDASVLSDMIFEMEFCSQWFMDNVQTEIRRHIPKEKKKESRMNADYYDR
Sbjct: 1   MSAAKANVASFFDASVLSDMIFEMEFCSQWFMDNVQTEIRRHIPKEKKKESRMNADYYDR 60

Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG
Sbjct: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 112

[Close]

BLAST of Csa4G189510 vs. TrEMBL
Match: A0A0A0KX92_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G296240 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 4.8e-16
Identity = 45/52 (86.54%), Postives = 48/52 (92.31%), Query Frame = 1


Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AEE EKKIKEEM EVYV+VVPP++AIT DLRFNRVWLFVDSSGKV QTPSIG
Sbjct: 53  AEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVDSSGKVRQTPSIG 104

[Close]

BLAST of Csa4G189510 vs. TrEMBL
Match: V7BVE7_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_005G032000g PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 2.8e-08
Identity = 32/52 (61.54%), Postives = 40/52 (76.92%), Query Frame = 1


Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AEE E KIKEEM+EV + VVPPD  +T+D +  RV L+VD S KVI+TP+IG
Sbjct: 44  AEEAETKIKEEMSEVQIQVVPPDSFVTADFKPKRVRLYVDESNKVIRTPAIG 95

[Close]

BLAST of Csa4G189510 vs. TrEMBL
Match: G7IW97_MEDTR (Subtilisin inhibitor 1 OS=Medicago truncatula GN=MTR_3g020970 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 4.9e-08
Identity = 31/52 (59.62%), Postives = 40/52 (76.92%), Query Frame = 1


Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AEE E+KIKE+++ V + VVPPD  +T+D RF RV L+VD S KVI+TP IG
Sbjct: 47  AEEAERKIKEDISGVEIQVVPPDSFVTADFRFKRVRLYVDESNKVIRTPIIG 98

[Close]

BLAST of Csa4G189510 vs. TrEMBL
Match: A0A067EAY7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g034123mg PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.4e-07
Identity = 32/52 (61.54%), Postives = 37/52 (71.15%), Query Frame = 1


Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AEE EKKIKEE     V VVPP+  +T D R NRV L+VDSSGKV + PS+G
Sbjct: 52  AEEAEKKIKEERPGAQVQVVPPNNFVTMDFRHNRVRLYVDSSGKVERPPSVG 103

[Close]

BLAST of Csa4G189510 vs. NCBI nr
Match: gi|700198770|gb|KGN53928.1| (hypothetical protein Csa_4G189510 [Cucumis sativus])

HSP 1 Score: 226.5 bits (576), Expect = 2.4e-56
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 1


Query: 1   MSAAKANVASFFDASVLSDMIFEMEFCSQWFMDNVQTEIRRHIPKEKKKESRMNADYYDR 60
           MSAAKANVASFFDASVLSDMIFEMEFCSQWFMDNVQTEIRRHIPKEKKKESRMNADYYDR
Sbjct: 1   MSAAKANVASFFDASVLSDMIFEMEFCSQWFMDNVQTEIRRHIPKEKKKESRMNADYYDR 60

Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG
Sbjct: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 112

[Close]

BLAST of Csa4G189510 vs. NCBI nr
Match: gi|778697662|ref|XP_011654371.1| (PREDICTED: subtilisin inhibitor CLSI-I-like [Cucumis sativus])

HSP 1 Score: 92.0 bits (227), Expect = 6.9e-16
Identity = 45/52 (86.54%), Postives = 48/52 (92.31%), Query Frame = 1


Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AEE EKKIKEEM EVYV+VVPP++AIT DLRFNRVWLFVDSSGKV QTPSIG
Sbjct: 53  AEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVDSSGKVRQTPSIG 104

[Close]

BLAST of Csa4G189510 vs. NCBI nr
Match: gi|593697034|ref|XP_007148999.1| (hypothetical protein PHAVU_005G032000g [Phaseolus vulgaris])

HSP 1 Score: 66.2 bits (160), Expect = 4.1e-08
Identity = 32/52 (61.54%), Postives = 40/52 (76.92%), Query Frame = 1


Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AEE E KIKEEM+EV + VVPPD  +T+D +  RV L+VD S KVI+TP+IG
Sbjct: 44  AEEAETKIKEEMSEVQIQVVPPDSFVTADFKPKRVRLYVDESNKVIRTPAIG 95

[Close]

BLAST of Csa4G189510 vs. NCBI nr
Match: gi|357457017|ref|XP_003598789.1| (subtilisin inhibitor 1 [Medicago truncatula])

HSP 1 Score: 65.5 bits (158), Expect = 7.0e-08
Identity = 31/52 (59.62%), Postives = 40/52 (76.92%), Query Frame = 1


Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           AEE E+KIKE+++ V + VVPPD  +T+D RF RV L+VD S KVI+TP IG
Sbjct: 47  AEEAERKIKEDISGVEIQVVPPDSFVTADFRFKRVRLYVDESNKVIRTPIIG 98

[Close]

BLAST of Csa4G189510 vs. NCBI nr
Match: gi|1012207901|ref|XP_015932366.1| (PREDICTED: subtilisin inhibitor CLSI-I-like [Arachis duranensis])

HSP 1 Score: 65.1 bits (157), Expect = 9.1e-08
Identity = 30/52 (57.69%), Postives = 39/52 (75.00%), Query Frame = 1


Query: 61  AEEVEKKIKEEMTEVYVSVVPPDYAITSDLRFNRVWLFVDSSGKVIQTPSIG 113
           +EE +KKIKEEMTE  + V+PP Y +T+D R  RV ++VD S KVI+TP IG
Sbjct: 47  SEEAKKKIKEEMTEADIQVIPPGYFVTADFRSQRVRIYVDQSDKVIRTPIIG 98

The following BLAST results are available for this feature:

BLAST of Csa4G189510 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ICI1_PHAAN	3.5e-07	53.85	Subtilisin inhibitor 1 OS=Phaseolus angularis PE=1 SV=1	[more]
ICI1_CANLI	1.7e-06	55.77	Subtilisin inhibitor CLSI-I OS=Canavalia lineata PE=1 SV=1	[more]
ICIS_VICFA	3.8e-06	50.00	Subtilisin inhibitor (Fragment) OS=Vicia faba PE=1 SV=1	[more]

BLAST of Csa4G189510 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWM2_CUCSA	1.6e-56	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G189510 PE=4 SV=1	[more]
A0A0A0KX92_CUCSA	4.8e-16	86.54	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G296240 PE=4 SV=1	[more]
V7BVE7_PHAVU	2.8e-08	61.54	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_005G032000g PE=4 SV=1	[more]
G7IW97_MEDTR	4.9e-08	59.62	Subtilisin inhibitor 1 OS=Medicago truncatula GN=MTR_3g020970 PE=4 SV=1	[more]
A0A067EAY7_CITSI	2.4e-07	61.54	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g034123mg PE=4 SV=1	[more]

BLAST of Csa4G189510 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700198770\|gb\|KGN53928.1\|	2.4e-56	100.00	hypothetical protein Csa_4G189510 [Cucumis sativus]	[more]
gi\|778697662\|ref\|XP_011654371.1\|	6.9e-16	86.54	PREDICTED: subtilisin inhibitor CLSI-I-like [Cucumis sativus]	[more]
gi\|593697034\|ref\|XP_007148999.1\|	4.1e-08	61.54	hypothetical protein PHAVU_005G032000g [Phaseolus vulgaris]	[more]
gi\|357457017\|ref\|XP_003598789.1\|	7.0e-08	59.62	subtilisin inhibitor 1 [Medicago truncatula]	[more]
gi\|1012207901\|ref\|XP_015932366.1\|	9.1e-08	57.69	PREDICTED: subtilisin inhibitor CLSI-I-like [Arachis duranensis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000864	Prot_inh_pot1

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0009611	response to wounding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU171904	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa4G189510.1	Csa4G189510.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU171904	CU171904	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRODOM	PD002604		coord: 61..112 score: 8.
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 61..112 score: 1.2
IPR000864	Proteinase inhibitor I13, potato inhibitor I	unknown	SSF54654	CI-2 family of serine protease inhibitors	coord: 61..112 score: 1.7
None	No IPR available	unknown	Coil	Coil	coord: 54..74 scor
None	No IPR available	GENE3D	G3DSA:3.30.10.10		coord: 61..112 score: 8.6
None	No IPR available	PANTHER	PTHR33091	FAMILY NOT NAMED	coord: 61..112 score: 1.6
None	No IPR available	PANTHER	PTHR33091:SF11	SUBFAMILY NOT NAMED	coord: 61..112 score: 1.6

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa4G189510	Watermelon (97103) v2	cuwmbB299
Csa4G189510	Wax gourd	cuwgoB406
Csa4G189510	Cucumber (Gy14) v1	cgycuB473
Csa4G189510	Cucurbita maxima (Rimu)	cmacuB664
Csa4G189510	Cucurbita maxima (Rimu)	cmacuB860
Csa4G189510	Cucurbita moschata (Rifu)	cmocuB652
Csa4G189510	Cucurbita moschata (Rifu)	cmocuB848
Csa4G189510	Melon (DHL92) v3.5.1	cumeB324
Csa4G189510	Watermelon (Charleston Gray)	cuwcgB302
Csa4G189510	Watermelon (97103) v1	cuwmB339
Csa4G189510	Cucurbita pepo (Zucchini)	cpecuB079
Csa4G189510	Cucurbita pepo (Zucchini)	cpecuB497
Csa4G189510	Bottle gourd (USVL1VR-Ls)	culsiB258
Csa4G189510	Cucumber (Gy14) v2	cgybcuB168
Csa4G189510	Melon (DHL92) v3.6.1	cumedB311
Csa4G189510	Silver-seed gourd	carcuB0651