Csa4G105350 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa4G105350
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Alpha-amylase, putative; contains IPR015902 (Glycoside hydrolase, family 13), IPR017853 (Glycoside hydrolase, superfamily)
Location	Chr4 : 6734033 .. 6738273 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTTTTTGGGCATATAAAAAGGTGTCCTCTCCCCGCCATTCCTTCTCCTTTTTTCTTCATTACATTTTCAACTTCCACATCTGAAGAATGCAACTCCGTTTCTCCTTCTGATTTCGCCATTCAATTCAGGTTTTCAATCTTCTTATACTTGTTCTGTCTTCCCTTTTTACTTCTACACCTTCCAATTACCCTTCATTATCATCAGATTACTCACTTCTCCACTCCCAAGATCGTTTCTATTTCGTTTGTTGAATTTATTATAATCGGGGATTCAATTGTTCGCATTCAGTTGTTTTCTTTTGCGATTTCAGTAATATCATTCGTACCAGTGCCAGTGAACAGTTAGGCATAAATTATGTATCTCGTCCTTAACTTTGATTAATTTCAAATCTATAATTCAGTTTTCCTTTCTGTTGGGAGTAATGGGTCCATTCCCATTACTTGATGCGGCAATTGAAATCTCCCCGCGTTGCCCTATTATCACTTCGAGATCTTCATATGGCAGGCGGTCATCACATTGCCATCTCCGTTTAACTGCAGTTTCATCTACCAGGACCTGGTACTAGTACTTTTACTCTGCCTGAATGTCTTTTCCTCCGGAAAAAAATGGGGGTTGAGATTTTTGTTTTCTCCACTGCTTTTTCTATCCTCCTTTCTGTTCGATTACGATCAAATGGGTTGTGTTAATGCAGGAAAGTCTCATACATTGAAAACTTGCAGTCTAAACCAAAAACTGTTGCCTTTTCTAGTCGGGTAGGATCTTTAGAACTCACATTCTTTATATTTTCATTATTTAAATCTGCAAGCTTGCTGCTCTGATTAAACAATGTTTTATGAATGATCATATCAGTTCTTATTTAAATCTTATTTTTTTAAATTACTCTTGTAACATGGAAATGCCAGGCTAGAACATTTCCTGGTATGAAGATTGGAATTTAACTAATGTATTCAACTGCATCTTTACTTATGGATCTTTGTTTTGTCTTTTATGACAGGATAATTCAAATGATCATTTAACAGATTTGGTAAATGATGCTGATGGCTTTTCAACTGGAAGAAGTGAAGTATTAGAGACTGGAGAGGATGAAATTCTTGCAGTTAAAAAAGCGCTTTTGGAGTCTCAAACAAGACAAGAAGCAGTAGAGAAAGAGAGAGATCAACTACTTGAGAGGTTGGCTCGCTATGAGGCTAAACAAAAAGAATATGTTGCTACTATATTGCATGACAAGGAATTGGCAGTTTCAGAACTTGAGGGGGCCAGATCACTCTTTAATAAAAAACTCGAGGAATCAGTAGGTGAGAAGTTTGCTTTGGAGTCTAAGCTGGTTCTTGCGAAGCAGGATGCTATTGATCTTGCAGTGCAAGTTGAAAAGTTAGCCGCAATTGCCTTCCAGCAGGCCACCTCGCACATATTGGAAGATGCTCAATATAGAGTATCAGTTGCAGAAACTTCTGCTATTGAGACGTCTTATGAAATTGAAAAACAGATCAGGGATGCTACTGAAGGTTCAATGCTGTCATTTCTAGAACAATCCAAAATAGCTATTGAGAAGGCTTTGGATGTGGCTGAAAAAGCTAGTGCTCATGCAAAGAAGGCTATGGCTACATTTACTGATGAGGTATATCCTTTAGATGAGATTGCCTCTATCCAATCAGAAAATATTAAGTTGAAAGGTGTCATCAATGAATTGGAATCTCACTTATCACTTGCAAGAAGTAATGTCAATAACCTCAAATTGGAACTGGAGCAAGCCCGAGCACAAGCAACTGCATCAGAAATTCGAGCCAAAAATGCTGAGAAAGTATTGGTCGAATTTCAGGAGTTGAGCAGGGAAAAGATCAATCAACAGGAAGGAGAAATTAAATTGATGATGGAGAAAATCAAGAAAGATGTAGCAGACAAGAAGAAAGCTGCTTCCAAAGTTTTCAAGGCTGAACTTGAAGGTATTAAGTCTGCCATTCAAGCTGCCAAAGAAACTGCACACTCAAAAGACAGCGCATATATGAGAAGATGTGAAGCACTGCAAAGATTATTGAGGGCATCAGAAGCTGGAACAAAGATGTGGCAACAACGGGCTGATATGGCTGAGTCATTTTTATTGAAGGAAAGAACCATGGGTAAAGATAATGAAGATGCAGCTTATATTGTCAATGGTGGGCGGATAGACCTCCTAACAGATGATGAGTCACAAAAGTGGAAACTCTTGAGCGATGGTCCTCGAAGAGAGATACCTCAATGGATGGCTAGAAGAATCGGAACAATTCGTCCCAAGTTTCCTCCTAGAAAGATTGATGTAACTGAAATTTCAGTATCAAAGTTTAGATCATTGGACTTGCCCAAACTTGAGGAAGTATGGTCAATTGCTCAAGAAAAGCCAAAAGTAGGGGATACACTAATTGAGCATGTTATTGAGAAAGAAACAATAGAAAAGAAGAGAAAGGCTCTTGAACGTGCGCTGCAACGAAAGACCATACAATGGCAGAGGACGCCTGATCAAACAAAATTAGGTTAGCTGGCTTTATAATTTTTCCTTTTAATGTAATCAAGAAAATTTTAATGGTATTCATCTAACATTTAACTCTTGTCGACTGATGCAGAACCAGGAACTGGAACTGGACACGAGATTGTGGTTAGTTATCACTAATGATATCAGCAAACAGATGTATTTTACTCTGATTTACATAATTAAATTATTAGTTGTTCAAACTCAAAAAAATAATTTGGAAATAATAAGGTTTATTGTTTCAGAATTTGTTCAACTTGAGTTTATCAGGATTTTGTAATGTTTCATGGTTGCATCACACTTACTCATGTTTAATATAAATGACTTTGGTGATTGATTTTGTTGTAGTTGATCTCTCGTTCTTTAGTAAGGGATAGGTGTCACATCACACCGTACTAAAGTTTAGAGGGTATAACATGTCTTGGGCTGGCCTTGGACTAAGAGGCTAAACAGTGCTCTAGCGAAGGGGTTTGACAAGTTGGTAAACCATGTAAGTAGGGATGATAAGCATTCTAGGAAAAACAGAATTTCACGTGGAACACCGGGAGAGGAATAAGAGCGAGACCAGCCCTCTGTGGGACCCACTAGTTAGTTAGGATAAATAGAAGACGATGGAGAGAGAGAAAGGGGTCTGGAAGAAAAGAAGAAGAGGCTGCATTCCCTTGCGAGGGAGAAAGGCAGAGGGGTTCAGGTTTTAATTGTTGGGTCTTGATTTTAGGTTTATAGTATTGTGCTTACCTTTTACGTTTTGGTTGTCATTTTGACGTTGATTGCTGTTCTGCTGTGAACTGGAATTTTCAAGTTACTTCTAAGTTCTATATTGTCTGGTTCTGGAATTTTATATTGTATCTGTTGAGGTAAAACAATATATATATAAAGATATATTTGTAGTATCTTTACAGGAAGTAACTGTGAGGAAGTTGAGTGTGCTAGGAATGTAGTTCCAACTGGGAAACAACTACTGTTTGGATAAAAACAACGGGAACGGTGTCTTGTACAGAGATGTCATTATCATGTCTTAAGCAGTTAGAAAACCTTCTAAGAGTGTGTTAATCTTCCTCATGCCACGTCAAATATAAATGATGTTAGAATCTTGTAGGAATTTTCTTTTTTTCAGTGCTTTATCGTTAAATGTTCCCCATTGAAAACCTATTATTTTTTCAGTTATATAATATGTGCACCAACATTAACTGATGATTGTTTATGGGTTCCTCTCGAAGTTTCAAGGTTTCAACTGGGAAAGCTGGAGAAGACGCTGGTACCTGGAGTTAGCTGCTAAAGCATCTGATTTATCTCAGTCTGGAATAACAGCAGTGTGGCTCCCACCACCGACGGAGTCTGTTGCTCCACAAGGTTTGTTCCATGTCATTTTTATTTTATATAGTCTATGGGGGAAGATAATATCAATAAGCTCTTCTTCTATTCTTCAACACTTCTAGACTCTTTTTTCCTCAATTTGGTTCACGTGGGATAATTTCAAGTACTGAACACAGTGGGGAATATTTTCCTTTTCTTAAAAATCCAATTTCGTTAAAATTTACAAACAGTGGTGTATATTTTGCAGGTTATATGCCATCTGACCTATATAATCTGAACTCATCTTACGGAACAGTGGAGGAACTCAAGTACTGTATAGAAGAATTTCATTCTCAAGATCTTCTGGTATGCCGTGGTCTGAAACTTTTCCTATGTATTGCATATTTATTTAACTCCTCTATTTGTTTCAATTATCCATGA

mRNA sequence

ATGGGTCCATTCCCATTACTTGATGCGGCAATTGAAATCTCCCCGCGTTGCCCTATTATCACTTCGAGATCTTCATATGGCAGGCGGTCATCACATTGCCATCTCCGTTTAACTGCAGTTTCATCTACCAGGACCTGGAAAGTCTCATACATTGAAAACTTGCAGTCTAAACCAAAAACTGTTGCCTTTTCTAGTCGGGATAATTCAAATGATCATTTAACAGATTTGGTAAATGATGCTGATGGCTTTTCAACTGGAAGAAGTGAAGTATTAGAGACTGGAGAGGATGAAATTCTTGCAGTTAAAAAAGCGCTTTTGGAGTCTCAAACAAGACAAGAAGCAGTAGAGAAAGAGAGAGATCAACTACTTGAGAGGTTGGCTCGCTATGAGGCTAAACAAAAAGAATATGTTGCTACTATATTGCATGACAAGGAATTGGCAGTTTCAGAACTTGAGGGGGCCAGATCACTCTTTAATAAAAAACTCGAGGAATCAGTAGGTGAGAAGTTTGCTTTGGAGTCTAAGCTGGTTCTTGCGAAGCAGGATGCTATTGATCTTGCAGTGCAAGTTGAAAAGTTAGCCGCAATTGCCTTCCAGCAGGCCACCTCGCACATATTGGAAGATGCTCAATATAGAGTATCAGTTGCAGAAACTTCTGCTATTGAGACGTCTTATGAAATTGAAAAACAGATCAGGGATGCTACTGAAGGTTCAATGCTGTCATTTCTAGAACAATCCAAAATAGCTATTGAGAAGGCTTTGGATGTGGCTGAAAAAGCTAGTGCTCATGCAAAGAAGGCTATGGCTACATTTACTGATGAGGTATATCCTTTAGATGAGATTGCCTCTATCCAATCAGAAAATATTAAGTTGAAAGGTGTCATCAATGAATTGGAATCTCACTTATCACTTGCAAGAAGTAATGTCAATAACCTCAAATTGGAACTGGAGCAAGCCCGAGCACAAGCAACTGCATCAGAAATTCGAGCCAAAAATGCTGAGAAAGTATTGGTCGAATTTCAGGAGTTGAGCAGGGAAAAGATCAATCAACAGGAAGGAGAAATTAAATTGATGATGGAGAAAATCAAGAAAGATGTAGCAGACAAGAAGAAAGCTGCTTCCAAAGTTTTCAAGGCTGAACTTGAAGGTATTAAGTCTGCCATTCAAGCTGCCAAAGAAACTGCACACTCAAAAGACAGCGCATATATGAGAAGATGTGAAGCACTGCAAAGATTATTGAGGGCATCAGAAGCTGGAACAAAGATGTGGCAACAACGGGCTGATATGGCTGAGTCATTTTTATTGAAGGAAAGAACCATGGGTAAAGATAATGAAGATGCAGCTTATATTGTCAATGGTGGGCGGATAGACCTCCTAACAGATGATGAGTCACAAAAGTGGAAACTCTTGAGCGATGGTCCTCGAAGAGAGATACCTCAATGGATGGCTAGAAGAATCGGAACAATTCGTCCCAAGTTTCCTCCTAGAAAGATTGATGTAACTGAAATTTCAGTATCAAAGTTTAGATCATTGGACTTGCCCAAACTTGAGGAAGTATGGTCAATTGCTCAAGAAAAGCCAAAAGTAGGGGATACACTAATTGAGCATGTTATTGAGAAAGAAACAATAGAAAAGAAGAGAAAGGCTCTTGAACGTGCGCTGCAACGAAAGACCATACAATGGCAGAGGACGCCTGATCAAACAAAATTAGAACCAGGAACTGGAACTGGACACGAGATTGTGTTTCAAGGTTTCAACTGGGAAAGCTGGAGAAGACGCTGGTACCTGGAGTTAGCTGCTAAAGCATCTGATTTATCTCAGTCTGGAATAACAGCAGTGTGGCTCCCACCACCGACGGAGTCTGTTGCTCCACAAGGTTATATGCCATCTGACCTATATAATCTGAACTCATCTTACGGAACAGTGGAGGAACTCAAGTACTGTATAGAAGAATTTCATTCTCAAGATCTTCTGGTATGCCGTGGTCTGAAACTTTTCCTATGTATTGCATATTTATTTAACTCCTCTATTTGTTTCAATTATCCATGA

Coding sequence (CDS)

ATGGGTCCATTCCCATTACTTGATGCGGCAATTGAAATCTCCCCGCGTTGCCCTATTATCACTTCGAGATCTTCATATGGCAGGCGGTCATCACATTGCCATCTCCGTTTAACTGCAGTTTCATCTACCAGGACCTGGAAAGTCTCATACATTGAAAACTTGCAGTCTAAACCAAAAACTGTTGCCTTTTCTAGTCGGGATAATTCAAATGATCATTTAACAGATTTGGTAAATGATGCTGATGGCTTTTCAACTGGAAGAAGTGAAGTATTAGAGACTGGAGAGGATGAAATTCTTGCAGTTAAAAAAGCGCTTTTGGAGTCTCAAACAAGACAAGAAGCAGTAGAGAAAGAGAGAGATCAACTACTTGAGAGGTTGGCTCGCTATGAGGCTAAACAAAAAGAATATGTTGCTACTATATTGCATGACAAGGAATTGGCAGTTTCAGAACTTGAGGGGGCCAGATCACTCTTTAATAAAAAACTCGAGGAATCAGTAGGTGAGAAGTTTGCTTTGGAGTCTAAGCTGGTTCTTGCGAAGCAGGATGCTATTGATCTTGCAGTGCAAGTTGAAAAGTTAGCCGCAATTGCCTTCCAGCAGGCCACCTCGCACATATTGGAAGATGCTCAATATAGAGTATCAGTTGCAGAAACTTCTGCTATTGAGACGTCTTATGAAATTGAAAAACAGATCAGGGATGCTACTGAAGGTTCAATGCTGTCATTTCTAGAACAATCCAAAATAGCTATTGAGAAGGCTTTGGATGTGGCTGAAAAAGCTAGTGCTCATGCAAAGAAGGCTATGGCTACATTTACTGATGAGGTATATCCTTTAGATGAGATTGCCTCTATCCAATCAGAAAATATTAAGTTGAAAGGTGTCATCAATGAATTGGAATCTCACTTATCACTTGCAAGAAGTAATGTCAATAACCTCAAATTGGAACTGGAGCAAGCCCGAGCACAAGCAACTGCATCAGAAATTCGAGCCAAAAATGCTGAGAAAGTATTGGTCGAATTTCAGGAGTTGAGCAGGGAAAAGATCAATCAACAGGAAGGAGAAATTAAATTGATGATGGAGAAAATCAAGAAAGATGTAGCAGACAAGAAGAAAGCTGCTTCCAAAGTTTTCAAGGCTGAACTTGAAGGTATTAAGTCTGCCATTCAAGCTGCCAAAGAAACTGCACACTCAAAAGACAGCGCATATATGAGAAGATGTGAAGCACTGCAAAGATTATTGAGGGCATCAGAAGCTGGAACAAAGATGTGGCAACAACGGGCTGATATGGCTGAGTCATTTTTATTGAAGGAAAGAACCATGGGTAAAGATAATGAAGATGCAGCTTATATTGTCAATGGTGGGCGGATAGACCTCCTAACAGATGATGAGTCACAAAAGTGGAAACTCTTGAGCGATGGTCCTCGAAGAGAGATACCTCAATGGATGGCTAGAAGAATCGGAACAATTCGTCCCAAGTTTCCTCCTAGAAAGATTGATGTAACTGAAATTTCAGTATCAAAGTTTAGATCATTGGACTTGCCCAAACTTGAGGAAGTATGGTCAATTGCTCAAGAAAAGCCAAAAGTAGGGGATACACTAATTGAGCATGTTATTGAGAAAGAAACAATAGAAAAGAAGAGAAAGGCTCTTGAACGTGCGCTGCAACGAAAGACCATACAATGGCAGAGGACGCCTGATCAAACAAAATTAGAACCAGGAACTGGAACTGGACACGAGATTGTGTTTCAAGGTTTCAACTGGGAAAGCTGGAGAAGACGCTGGTACCTGGAGTTAGCTGCTAAAGCATCTGATTTATCTCAGTCTGGAATAACAGCAGTGTGGCTCCCACCACCGACGGAGTCTGTTGCTCCACAAGGTTATATGCCATCTGACCTATATAATCTGAACTCATCTTACGGAACAGTGGAGGAACTCAAGTACTGTATAGAAGAATTTCATTCTCAAGATCTTCTGGTATGCCGTGGTCTGAAACTTTTCCTATGTATTGCATATTTATTTAACTCCTCTATTTGTTTCAATTATCCATGA

Protein sequence

MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKTVAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERDQLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAKQDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSMLSFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELESHLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMMEKIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGTKMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQWMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEKETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLLVCRGLKLFLCIAYLFNSSICFNYP*

Homology

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BLAST of Csa4G105350 vs. Swiss-Prot
Match: AMY3_ARATH (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana GN=AMY3 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.8e-26
Identity = 69/176 (39.20%), Postives = 100/176 (56.82%), Query Frame = 1


Query: 494 PPRKIDVTEISVSKFRSLDLPKLE----EVWSIAQEKPKVGDTLIEHVIEKETIEKKRKA 553
           P RK D  E+S S F    + ++     ++ S   +K  V +     + E E +  +  +
Sbjct: 403 PKRKTD-KEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYS 462

Query: 554 LERAL-----QRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRR-RWYLELAAKAS 613
           + R+      +   ++ +      K+  GTG+G EI+ QGFNWES +  RWYLEL  KA 
Sbjct: 463 IFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKAD 522

Query: 614 DLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLLV 660
           +L+  G T +WLPPPTESV+P+GYMP DLYNLNS YGT++ELK  +++FH   + V
Sbjct: 523 ELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKV 577

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BLAST of Csa4G105350 vs. Swiss-Prot
Match: AMY3A_ORYSJ (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 97.8 bits (242), Expect = 4.8e-19
Identity = 44/82 (53.66%), Postives = 59/82 (71.95%), Query Frame = 1


Query: 579 EIVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLN 638
           +I+FQGFNW+SW+++  WY  L  +  D++ +G+T VWLPPPT SV+PQGYMP  LY+LN
Sbjct: 29  QILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLN 88

Query: 639 SS-YGTVEELKYCIEEFHSQDL 658
           +S YGT  ELK  I  FH++ +
Sbjct: 89  ASKYGTKAELKSLIAAFHAKGI 110

[Close]

BLAST of Csa4G105350 vs. Swiss-Prot
Match: AMY3D_ORYSJ (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 8.2e-19
Identity = 43/80 (53.75%), Postives = 58/80 (72.50%), Query Frame = 1


Query: 579 EIVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLN 638
           +++FQGFNWESW+++  WY  L  +  D++++G+T VWLPPP+ SVAPQGYMP  LY+L+
Sbjct: 26  QVLFQGFNWESWKQQGGWYNMLKGQVDDIAKAGVTHVWLPPPSHSVAPQGYMPGRLYDLD 85

Query: 639 SS-YGTVEELKYCIEEFHSQ 656
           +S YGT  ELK  I  FH +
Sbjct: 86  ASKYGTAAELKSLIAAFHGK 105

[Close]

BLAST of Csa4G105350 vs. Swiss-Prot
Match: AMY3E_ORYSJ (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.1e-18
Identity = 44/82 (53.66%), Postives = 59/82 (71.95%), Query Frame = 1


Query: 579 EIVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLN 638
           +++FQGFNWESWR++  WY  L  K  +++ +G T VWLPPP+ SV+PQGYMP  LY+L+
Sbjct: 26  QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85

Query: 639 SS-YGTVEELKYCIEEFHSQDL 658
           +S YGT  ELK  IE FH +++
Sbjct: 86  ASKYGTEAELKSLIEAFHDKNV 107

[Close]

BLAST of Csa4G105350 vs. Swiss-Prot
Match: AMY3B_ORYSJ (Alpha-amylase isozyme 3B OS=Oryza sativa subsp. japonica GN=AMY1.6 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.1e-18
Identity = 49/106 (46.23%), Postives = 68/106 (64.15%), Query Frame = 1


Query: 579 EIVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLN 638
           +++FQGFNWESW+++  WY  L     D++ +G+T VWLPPP+ SVAPQGYMP  LY+L+
Sbjct: 27  QVLFQGFNWESWKKQGGWYNFLHGHVDDIAATGVTHVWLPPPSHSVAPQGYMPGRLYDLD 86

Query: 639 SS-YGTVEELKYCIEEFHSQDLLVCRGLKLFLCIAYLFNSSICFNY 682
           +S YGT  EL+  I  FHS      +G+K   C+A +  +  C +Y
Sbjct: 87  ASKYGTGAELRSLIAAFHS------KGIK---CVADIVINHRCADY 123

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BLAST of Csa4G105350 vs. TrEMBL
Match: B9IK70_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s12870g PE=4 SV=2)

HSP 1 Score: 849.0 bits (2192), Expect = 4.1e-243
Identity = 433/627 (69.06%), Postives = 523/627 (83.41%), Query Frame = 1


Query: 32  HCHLRLTAVSSTRTWKVSYIENLQSKPKTVAFSSRDNSNDHLTDLVNDADGFSTGRSEVL 91
           H H  +   + TR  ++    N   K +TV FS+ D+SND   D+V+D +GF    +E L
Sbjct: 29  HHHSLIFPPARTRKRRLLSNGNWNRKSRTVVFSTVDDSNDSSADMVDDDNGFMLRGTEDL 88

Query: 92  ETGEDEILAVKKALLESQTRQEAVEKERDQLLERLARYEAKQKEYVATILHDKELAVSEL 151
              E+E++A KKAL E++ RQEA+EKERDQLLE LA+ +AKQ+E+VATIL DKE+A++EL
Sbjct: 89  VIEENELVATKKALSEARARQEAIEKERDQLLEELAQSQAKQQEHVATILRDKEVAITEL 148

Query: 152 EGARSLFNKKLEESVGEKFALESKLVLAKQDAIDLAVQVEKLAAIAFQQATSHILEDAQY 211
           E A+SLF+ KL++SV EKF LESKLVLAKQDA++LAVQVEKLA IAFQQATSHILEDAQ 
Sbjct: 149 EAAKSLFHNKLQDSVEEKFTLESKLVLAKQDAVELAVQVEKLAEIAFQQATSHILEDAQR 208

Query: 212 RVSVAETSAIETSYEIEKQIRDATEGSMLSFLEQSKIAIEKALDVAEKASAHAKKAMATF 271
           RVS AETSA E ++ IE+Q+R ATEG++LS +EQSK AIEKALDVAEKA  +A +A+A F
Sbjct: 209 RVSAAETSAAEAAFHIEEQVRIATEGTILSIVEQSKDAIEKALDVAEKAGDYATRAVAVF 268

Query: 272 TDEVYPLDEIASIQSENIKLKGVINELESHLSLARSNVNNLKLELEQARAQATASEIRAK 331
           TD + P+DEIAS+QSENIKL+G++N+LES L + R+ +  LK+ELEQ  AQA  SE+ A+
Sbjct: 269 TDGINPVDEIASVQSENIKLQGIVNDLESQLLITRNEIAKLKVELEQVNAQAKTSELHAE 328

Query: 332 NAEKVLVEFQELSREKINQQEGEIKLMMEKIKKDVADKKKAASKVFKAELEGIKSAIQAA 391
           +AEK L+EFQE +RE   Q+E EI  ++EK+KKD A+KKKAASK FKA+LE IK+AI+AA
Sbjct: 329 DAEKALLEFQESNREMTIQREEEINSLLEKMKKDAAEKKKAASKAFKAQLESIKAAIKAA 388

Query: 392 KETAHSKDSAYMRRCEALQRLLRASEAGTKMWQQRADMAESFLLKERTMGKDNEDAAYIV 451
           KETAHS++ AYMRRCEALQR LRASEA +KMW+ RA++AES LLKE    +++EDA YIV
Sbjct: 389 KETAHSRNEAYMRRCEALQRSLRASEAASKMWKHRAEIAESLLLKE----EEDEDAIYIV 448

Query: 452 NGGRIDLLTDDESQKWKLLSDGPRREIPQWMARRIGTIRPKFPPRKIDVTEISVSKFRSL 511
           NGGRIDLLTDD+SQKWKLLSDGPRRE P WMARRI +IRPKFPPRKIDV+E   S FR L
Sbjct: 449 NGGRIDLLTDDDSQKWKLLSDGPRRETPHWMARRIRSIRPKFPPRKIDVSEALTSNFRPL 508

Query: 512 DLPKLEEVWSIAQEKPKVGDTLIEHVIEKETIEKKRKALERALQRKTIQWQRTPDQTKLE 571
           DLPK +EVWSIAQEK K  DTLIEHVIEKETIEKKRKALERALQRKTIQWQ+TP++TKLE
Sbjct: 509 DLPKPDEVWSIAQEKLKERDTLIEHVIEKETIEKKRKALERALQRKTIQWQKTPEETKLE 568

Query: 572 PGTGTGHEIVFQGFNWESWRRRWYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSD 631
           PGTGTG EIVFQGFNWESWR++WYL+LA KA+DLS+ G+TAVWLPPPTESVAPQGYMPSD
Sbjct: 569 PGTGTGREIVFQGFNWESWRKQWYLDLAPKAADLSRCGVTAVWLPPPTESVAPQGYMPSD 628

Query: 632 LYNLNSSYGTVEELKYCIEEFHSQDLL 659
           LYNLNS+YG+VEELK+C+EE HSQDLL
Sbjct: 629 LYNLNSAYGSVEELKHCVEEMHSQDLL 651

[Close]

BLAST of Csa4G105350 vs. TrEMBL
Match: B9SJF1_RICCO (Alpha-amylase, putative OS=Ricinus communis GN=RCOM_0526780 PE=4 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 1.6e-242
Identity = 427/619 (68.98%), Postives = 518/619 (83.68%), Query Frame = 1


Query: 42  STRTWK--VSYIENLQSKPKTVAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEIL 101
           S+ TWK  + Y  +   K +TV  SS + SND  T +VN  D  S+ R++VLE  E+E++
Sbjct: 43  SSYTWKRRLFYNGSWHCKSRTVVLSSMEESNDTFTGVVNSGDDLSSQRAQVLEDEENELV 102

Query: 102 AVKKALLESQTRQEAVEKERDQLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFN 161
           A KKAL + +++QE +EKERD LLE LAR EAKQKEYVATIL DKELA+SELE A+SLF+
Sbjct: 103 AAKKALSDVRSKQETLEKERDLLLEELARSEAKQKEYVATILQDKELAISELEAAKSLFH 162

Query: 162 KKLEESVGEKFALESKLVLAKQDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETS 221
           +KL+++V EKFALES+LVLAKQDA++LAVQVEKL  IAFQQATSHILEDAQ RV+ AET+
Sbjct: 163 QKLQKTVEEKFALESRLVLAKQDAVELAVQVEKLTEIAFQQATSHILEDAQMRVAAAETT 222

Query: 222 AIETSYEIEKQIRDATEGSMLSFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLD 281
           A E +++IE+QIR+ATEG++ + ++QSK AI+KALDVAEKA  HA KA+A F+D   P D
Sbjct: 223 AAEAAFQIEEQIRNATEGTIFTIVQQSKDAIDKALDVAEKAGDHAAKAVAIFSDGANPFD 282

Query: 282 EIASIQSENIKLKGVINELESHLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVE 341
           EIASI+SENI+L+GV+N+LESHL + RS ++ LK EL+Q R+QA ASE+RA NAEK L+E
Sbjct: 283 EIASIKSENIRLEGVVNDLESHLLITRSEIDKLKAELDQVRSQAKASEVRANNAEKTLLE 342

Query: 342 FQELSREKINQQEGEIKLMMEKIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKD 401
           FQ+ +REK  QQE EI  ++EK++KD +++KKAASK FK+E+E IK+AI+AAKETA S++
Sbjct: 343 FQKSNREKAMQQEEEISSLLEKMRKDASERKKAASKAFKSEVESIKAAIEAAKETARSRE 402

Query: 402 SAYMRRCEALQRLLRASEAGTKMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLL 461
           +AYMRRCE+LQR LRASE+  KMW+QRA+MAES +L      + +ED+  IVNGGRIDLL
Sbjct: 403 NAYMRRCESLQRSLRASESALKMWRQRAEMAESLILD----AEKDEDSISIVNGGRIDLL 462

Query: 462 TDDESQKWKLLSDGPRREIPQWMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEV 521
           TDD+SQKWKLLSDGPRREIPQWMARRI TIRPKFPPRK +++E     FR LDLPK +EV
Sbjct: 463 TDDDSQKWKLLSDGPRREIPQWMARRIRTIRPKFPPRKTNISEALTKNFRHLDLPKPDEV 522

Query: 522 WSIAQEKPKVGDTLIEHVIEKETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHE 581
           WSIAQEKPKVGDTLIEHV+EKETIEKKRKALER LQRKTIQWQRTP+ TKLEPGTGTG E
Sbjct: 523 WSIAQEKPKVGDTLIEHVMEKETIEKKRKALERVLQRKTIQWQRTPEHTKLEPGTGTGRE 582

Query: 582 IVFQGFNWESWRRRWYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSY 641
           IVFQGFNWESWRR+WY+ELA K +DLSQ G+TAVWLPPPTESVAPQGYMPSDLYNLNS+Y
Sbjct: 583 IVFQGFNWESWRRQWYVELATKMADLSQCGVTAVWLPPPTESVAPQGYMPSDLYNLNSAY 642

Query: 642 GTVEELKYCIEEFHSQDLL 659
           GT EELKYCIEE HS D+L
Sbjct: 643 GTEEELKYCIEEMHSHDIL 657

[Close]

BLAST of Csa4G105350 vs. TrEMBL
Match: M5WYH2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000951mg PE=4 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 2.0e-242
Identity = 443/658 (67.33%), Postives = 527/658 (80.09%), Query Frame = 1


Query: 1   MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKT 60
           MG   L D A+ I   CP   S + +    + C  R   +  T            SK K 
Sbjct: 1   MGSISLPDMAVGIIHHCPTAYSGAHWKYDKNLCLGRRPPIFRTA-----------SKRKK 60

Query: 61  VAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERD 120
                 D+S+D   D+V      ++GR+EVL   EDE++  +KAL E+Q RQEA+EKERD
Sbjct: 61  C-----DDSSDTFADVVE-----TSGRNEVLNIEEDELITARKALSEAQARQEAIEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLE+LA  EAKQ+EYVATILH+KELA++E+E A+SLF++KL+ESV EKF+LESKLVLAK
Sbjct: 121 QLLEKLACSEAKQQEYVATILHEKELAIAEVEAAKSLFHQKLQESVEEKFSLESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240
            DA++LAVQVEKLA IAFQQATSHILEDAQ RVS AET+A E +YEIEKQIRD TEGS+L
Sbjct: 181 NDAVELAVQVEKLAEIAFQQATSHILEDAQLRVSAAETAAAEAAYEIEKQIRDVTEGSIL 240

Query: 241 SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES 300
           S +EQSK AIEKALDVAEKA  HA KA++ FT+ + PLDE+ASIQS+NI L+GV+N+LES
Sbjct: 241 SIVEQSKYAIEKALDVAEKAGEHATKAVSEFTEGMNPLDELASIQSKNIMLQGVVNDLES 300

Query: 301 HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360
            L L RS+V+ LKLELE+A A A A E+RAK+AEK L+EFQE S++   Q+E EI  ++E
Sbjct: 301 QLLLTRSDVDRLKLELEKAHAHANAFELRAKDAEKALLEFQESSKKNTLQKEEEIMSLIE 360

Query: 361 KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT 420
           K+KKD +++KKA+SK FKAEL+ I+ AI AAKE AHSKD AY+RRCEALQR L+ASEA T
Sbjct: 361 KMKKDSSERKKASSKAFKAELQSIRDAIGAAKEMAHSKDDAYLRRCEALQRSLKASEATT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMW+QRA+MAES L +ER +G+ +ED+ Y+VNGGRIDLLTDD+SQKWKLLSDGPRREIPQ
Sbjct: 421 KMWRQRAEMAESILCEERPLGEGDEDSIYVVNGGRIDLLTDDDSQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMAR+I TIRP+FPPRKIDV E   SKFRSLDLPK  EVWSIAQEKPK GDTLIEHVIEK
Sbjct: 481 WMARKIRTIRPRFPPRKIDVAEALSSKFRSLDLPKPNEVWSIAQEKPKEGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALE ALQ KTIQWQ+TP+QT LE GTGTG EIVFQGFNWESWR++WYL+LA 
Sbjct: 541 ETIEKKRKALEHALQGKTIQWQKTPEQTNLESGTGTGREIVFQGFNWESWRKQWYLDLAP 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLL 659
           KA+DLS+ G+T+VW PPPTESVAPQGYMPSDLYNLNSSYG+VEELK+CI E HSQ LL
Sbjct: 601 KAADLSKIGVTSVWFPPPTESVAPQGYMPSDLYNLNSSYGSVEELKHCIGEMHSQGLL 637

[Close]

BLAST of Csa4G105350 vs. TrEMBL
Match: W9QIQ2_9ROSA (Alpha-amylase isozyme 2A OS=Morus notabilis GN=L484_021018 PE=4 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 1.4e-238
Identity = 426/598 (71.24%), Postives = 505/598 (84.45%), Query Frame = 1


Query: 62  AFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGE-DEILAVKKALLESQTRQEAVEKERD 121
           A   R +SND +TDLV+D  G+ +G SEVL TGE DE++  ++AL E++ +QEA++KERD
Sbjct: 24  AIGKRGDSNDAVTDLVDD--GYLSGGSEVLGTGEEDELMKARQALSEARAKQEAIQKERD 83

Query: 122 QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK 181
           QL+E LAR EAKQKEY+ TILHDKEL VSELE A+SLF++KL+ESV EKF+LESKLVLAK
Sbjct: 84  QLIEELARSEAKQKEYIDTILHDKELVVSELEAAKSLFHQKLQESVDEKFSLESKLVLAK 143

Query: 182 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 241
           QDA++LAVQVEK A IAFQQATSHILEDAQ RVS AETSA E +Y+IEKQI+DATEG++ 
Sbjct: 144 QDAVELAVQVEKFAEIAFQQATSHILEDAQLRVSAAETSAAEAAYQIEKQIKDATEGTIS 203

Query: 242 SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES 301
           S +EQSK AI KALDVAEKA  +A KA++ F   + P++EI S+QSEN+KLK ++N+LES
Sbjct: 204 SIVEQSKDAINKALDVAEKAGDYATKAVSAFGGGINPVEEIVSVQSENMKLKRIVNDLES 263

Query: 302 HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 361
            L L RS V+ LKLE+EQ R QA ASEIRA NAEK LVEFQE +R+K  QQE EIK ++E
Sbjct: 264 QLLLIRSEVDKLKLEMEQVREQANASEIRANNAEKELVEFQEANRKKALQQEEEIKSLLE 323

Query: 362 KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT 421
           K+KKD  ++KKAA+K FKAELE IK+AI+AAKETA S+D+AY+RRCEALQR L+ASE   
Sbjct: 324 KMKKDALERKKAATKAFKAELESIKAAIEAAKETASSRDTAYLRRCEALQRSLKASEDAL 383

Query: 422 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 481
           KMW+QRA++AES L+KE  + + ++D+ Y+VNGGRIDLLTDD+SQKWKLLS+GPRREIPQ
Sbjct: 384 KMWRQRANLAESLLVKESPLVEGDKDSIYVVNGGRIDLLTDDDSQKWKLLSNGPRREIPQ 443

Query: 482 WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 541
           W ARRI TIRPKFPPRKIDV E   S FR+LDLPK ++VWSIA+EK K GDTLIE V+EK
Sbjct: 444 WRARRIRTIRPKFPPRKIDVAEALTSDFRTLDLPKPDKVWSIAEEKLKDGDTLIEQVMEK 503

Query: 542 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 601
           ETIEKKRKALERALQRKTIQWQRTP+ TKLEPGTGTG EIVFQ FNWESWRR+WYLELAA
Sbjct: 504 ETIEKKRKALERALQRKTIQWQRTPEHTKLEPGTGTGREIVFQAFNWESWRRQWYLELAA 563

Query: 602 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLL 659
           KA+DLSQSG TAVW PPPT+SVA QGYMP+DLYNLNS YGT EELKYCIEE HS  +L
Sbjct: 564 KAADLSQSGATAVWFPPPTKSVAAQGYMPTDLYNLNSEYGTEEELKYCIEEMHSHHIL 619

[Close]

BLAST of Csa4G105350 vs. TrEMBL
Match: A0A0A0R208_MORAL (Alpha-amylase OS=Morus alba var. multicaulis PE=2 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 4.0e-238
Identity = 436/663 (65.76%), Postives = 533/663 (80.39%), Query Frame = 1


Query: 1   MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCH----LRLTAVSSTRTWKVSYIENLQS 60
           MG   L   A  I P   ++ S  + G+R  + +    +  +  +S R  K+S  + + S
Sbjct: 1   MGSTLLTQMAFGILPSNAVV-SPGAIGKRVLNLYTGRIIVCSKAASKRKKKISCKDQVIS 60

Query: 61  KPKTVAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGED-EILAVKKALLESQTRQEAV 120
           + ++  FSS  +SND +T LV+D  G+ +  SEVL TGED E++  ++AL E++ +QEA+
Sbjct: 61  RSRSRIFSSVGDSNDAVTHLVDD--GYLSCGSEVLGTGEDDELMKARQALSEARAKQEAI 120

Query: 121 EKERDQLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESK 180
           +KERDQL+E LAR EAKQKEY+ TILH+KEL VSELE A+SLF++KL++SV EKF+LESK
Sbjct: 121 QKERDQLIEELARSEAKQKEYIDTILHEKELVVSELEAAKSLFHQKLQKSVDEKFSLESK 180

Query: 181 LVLAKQDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDAT 240
           LVLAKQDA++LAVQVEK A IAFQQATSHILEDAQ RVS AETSA E +Y+IEKQI+DAT
Sbjct: 181 LVLAKQDAVELAVQVEKFAEIAFQQATSHILEDAQLRVSAAETSAAEAAYQIEKQIKDAT 240

Query: 241 EGSMLSFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVI 300
           EG++ S +EQSK AI KALDVAEKA  +A KA++ F   + P++EI S+QSEN+KLK ++
Sbjct: 241 EGTISSIVEQSKDAINKALDVAEKAGDYATKAVSAFGGGINPVEEIVSVQSENMKLKRIV 300

Query: 301 NELESHLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEI 360
           N+LES L L R  V+ LKL++EQ R QA ASEIRA NAEK LVE QE SR+K  QQE EI
Sbjct: 301 NDLESQLLLIRREVDKLKLKMEQVREQANASEIRANNAEKELVELQEASRKKAIQQEEEI 360

Query: 361 KLMMEKIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRA 420
           K ++EK+KKD  ++K+AA+K FKAELE IK+AI+AAKETA S+D+AY+RRCEALQR L+A
Sbjct: 361 KSLLEKMKKDALERKEAAAKAFKAELESIKAAIEAAKETASSRDTAYLRRCEALQRSLKA 420

Query: 421 SEAGTKMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPR 480
           SE    MW+QRA++AES L+KE  + + ++D+ Y+VNGGRIDLLTDD+SQKWKLLSDGPR
Sbjct: 421 SEDALTMWRQRANLAESLLVKESPLVEGDKDSIYVVNGGRIDLLTDDDSQKWKLLSDGPR 480

Query: 481 REIPQWMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIE 540
           REIPQW ARRI TIRPKFPPRKIDV E+  S FR+LDLPK ++VWSIA+EK K GDTLIE
Sbjct: 481 REIPQWRARRIRTIRPKFPPRKIDVAEVLTSDFRTLDLPKSDKVWSIAEEKLKDGDTLIE 540

Query: 541 HVIEKETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWY 600
            V+EKETIEKKRKALERALQRKTIQWQRTP+ TKLEPGTGTG EIVFQ FNWESWRR+WY
Sbjct: 541 QVMEKETIEKKRKALERALQRKTIQWQRTPEHTKLEPGTGTGREIVFQAFNWESWRRQWY 600

Query: 601 LELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQ 659
           LELAAKA+DLSQSG TAVWLPPPT+SVA QGYMP+DLYNLNS YGT EELKYCIEE HS 
Sbjct: 601 LELAAKAADLSQSGATAVWLPPPTKSVAAQGYMPTDLYNLNSEYGTEEELKYCIEEMHSH 660

[Close]

BLAST of Csa4G105350 vs. TAIR10
Match: AT1G69830.1 (AT1G69830.1 alpha-amylase-like 3)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-27
Identity = 69/176 (39.20%), Postives = 100/176 (56.82%), Query Frame = 1


Query: 494 PPRKIDVTEISVSKFRSLDLPKLE----EVWSIAQEKPKVGDTLIEHVIEKETIEKKRKA 553
           P RK D  E+S S F    + ++     ++ S   +K  V +     + E E +  +  +
Sbjct: 403 PKRKTD-KEVSASGFTKEIITEIRNLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYS 462

Query: 554 LERAL-----QRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRR-RWYLELAAKAS 613
           + R+      +   ++ +      K+  GTG+G EI+ QGFNWES +  RWYLEL  KA 
Sbjct: 463 IFRSTTPAFSEEGVLEAEADKPDIKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKAD 522

Query: 614 DLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLLV 660
           +L+  G T +WLPPPTESV+P+GYMP DLYNLNS YGT++ELK  +++FH   + V
Sbjct: 523 ELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKV 577

[Close]

BLAST of Csa4G105350 vs. TAIR10
Match: AT1G76130.1 (AT1G76130.1 alpha-amylase-like 2)

HSP 1 Score: 91.7 bits (226), Expect = 1.9e-18
Identity = 38/81 (46.91%), Postives = 54/81 (66.67%), Query Frame = 1


Query: 566 DQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAAKASDLSQSGITAVWLPPPTESVAPQ 625
           DQT +      G E++ Q +NWES +  W+  L  K  D+++SG T+ WLPPP++S+AP+
Sbjct: 13  DQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPE 72

Query: 626 GYMPSDLYNLNSSYGTVEELK 647
           GY+P DLY+LNS+YG+   LK
Sbjct: 73  GYLPQDLYSLNSAYGSEHLLK 93

[Close]

BLAST of Csa4G105350 vs. TAIR10
Match: AT4G25000.1 (AT4G25000.1 alpha-amylase-like)

HSP 1 Score: 83.2 bits (204), Expect = 6.9e-16
Identity = 38/81 (46.91%), Postives = 55/81 (67.90%), Query Frame = 1


Query: 580 IVFQGFNWESWRRR--WYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNS 639
           ++FQ FNWESW++   +Y  L     D++ +GIT +WLPPP++SVAP+GY+P  LY+LNS
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 640 S-YGTVEELKYCIEEFHSQDL 658
           S YG+  ELK  I+  + + +
Sbjct: 87  SKYGSEAELKSLIKALNQKGI 107

[Close]

BLAST of Csa4G105350 vs. TAIR10
Match: AT4G14760.1 (AT4G14760.1 kinase interacting (KIP1-like) family protein)

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-08
Identity = 77/337 (22.85%), Postives = 155/337 (45.99%), Query Frame = 1


Query: 77  VNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERDQLLERLARYEAKQKEY 136
           +N  DG S          + E+  +K+ LLE QT +EA+  +   +L +++R+E +  + 
Sbjct: 145 MNKLDGTSDSHE-----ADSEVETLKRTLLELQTEKEALNLQYQLILSKVSRFEKELNDA 204

Query: 137 VATIL-HDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAKQDAIDLAVQVEKLAA 196
              +   D+    +++E       K L+ES+ +        +L    AI+    +E  A+
Sbjct: 205 QKDVKGFDERACKADIE------IKILKESLAKLEVERDTGLLQYSQAIERIADLE--AS 264

Query: 197 IAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSMLSFLEQSKIAIEKALD 256
           I+  Q  +  L +   RVS AE  A+    E+ + ++   E  +L + +  ++     + 
Sbjct: 265 ISHGQEYAKGLTN---RVSEAEREAMSLKKELSR-LQSEKEAGLLRYNKSLEL-----IS 324

Query: 257 VAEKASAHAKKAMATFTDEVYPLD-EIASIQSENIKLKGVINELESHLSLARSNVNNLKL 316
             EK    A++++  F D+    + EI +++ E +KL  V  +L          ++ L+ 
Sbjct: 325 SLEKTIRDAEESVRVFRDQSEQAETEIKALKQELLKLNEVNEDLNVRYQQCLETISKLER 384

Query: 317 ELEQAR--AQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMMEKIKKDVADKKKA 376
           E+  A+  A+  +SE+ A  A+   VE Q    E  NQ    +K+  E +   ++ K + 
Sbjct: 385 EVSHAQDNAKRLSSEVLAGAAKIKTVEEQCALLESFNQ---TMKVEAENLAHKMSAKDQE 444

Query: 377 ASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEAL 410
            S+  + E+E +++ +Q  ++   S+  A +R  E+L
Sbjct: 445 LSQK-QNEIEKLQAVMQ-EEQLRFSELGASLRNLESL 454

[Close]

BLAST of Csa4G105350 vs. TAIR10
Match: AT2G32240.1 (AT2G32240.1 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 57.4 bits (137), Expect = 4.0e-08
Identity = 84/372 (22.58%), Postives = 156/372 (41.94%), Query Frame = 1


Query: 55   QSKPKTVAFSSRDN----SNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQT 114
            Q++ K++  SS       +N+ L   + + +G       ++ +G  E     K L E+  
Sbjct: 880  QAQEKSLQSSSESELLAETNNQLKIKIQELEG-------LIGSGSVEKETALKRLEEAIE 939

Query: 115  RQEAVEKERDQLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKF 174
            R    E E   L+E+L  +E + +EY       K+LA  E  G       +LE+++ +  
Sbjct: 940  RFNQKETESSDLVEKLKTHENQIEEY-------KKLA-HEASGVADTRKVELEDALSKLK 999

Query: 175  ALESKLVLAKQDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQ 234
             LES +         L  +   LA +  +      LE A +     E     ++ E EK+
Sbjct: 1000 NLESTIEELGAKCQGLEKESGDLAEVNLKLN----LELANHGSEANELQTKLSALEAEKE 1059

Query: 235  IRDATEGSMLSFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEI-ASIQSENI 294
             + A E      LE SK  IE   D+ ++ ++  +K  +  +      +++ A  QS   
Sbjct: 1060 -QTANE------LEASKTTIE---DLTKQLTSEGEKLQSQISSHTEENNQVNAMFQSTKE 1119

Query: 295  KLKGVINELESHLSLARSNVNNLKLELEQARAQA---TASEIRAKNAEKVLVEFQELSRE 354
            +L+ VI +LE  L++  S  + L  E+E+ RA A   +  E   +  EK L E +   +E
Sbjct: 1120 ELQSVIAKLEEQLTVESSKADTLVSEIEKLRAVAAEKSVLESHFEELEKTLSEVKAQLKE 1179

Query: 355  KI-NQQEGEIKLMMEKIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRR 414
             + N     +K+     K    +       V   ++  ++  +QAA+ +   +  A+ ++
Sbjct: 1180 NVENAATASVKVAELTSKLQEHEHIAGERDVLNEQVLQLQKELQAAQSSIDEQKQAHSQK 1222

Query: 415  CEALQRLLRASE 418
               L+  L+ S+
Sbjct: 1240 QSELESALKKSQ 1222

[Close]

BLAST of Csa4G105350 vs. NCBI nr
Match: gi|700198531|gb|KGN53689.1| (hypothetical protein Csa_4G105350 [Cucumis sativus])

HSP 1 Score: 1326.6 bits (3432), Expect = 0.0e+00
Identity = 682/682 (100.00%), Postives = 682/682 (100.00%), Query Frame = 1


Query: 1   MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKT 60
           MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKT
Sbjct: 1   MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKT 60

Query: 61  VAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERD 120
           VAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERD
Sbjct: 61  VAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240

Query: 241 SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES 300
           SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES
Sbjct: 241 SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES 300

Query: 301 HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360
           HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME
Sbjct: 301 HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT 420
           KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT
Sbjct: 361 KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ
Sbjct: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLLVC 660
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLLVC
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLLVC 660

Query: 661 RGLKLFLCIAYLFNSSICFNYP 683
           RGLKLFLCIAYLFNSSICFNYP
Sbjct: 661 RGLKLFLCIAYLFNSSICFNYP 682

[Close]

BLAST of Csa4G105350 vs. NCBI nr
Match: gi|449438801|ref|XP_004137176.1| (PREDICTED: uncharacterized protein LOC101217339 [Cucumis sativus])

HSP 1 Score: 1275.0 bits (3298), Expect = 0.0e+00
Identity = 658/658 (100.00%), Postives = 658/658 (100.00%), Query Frame = 1


Query: 1   MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKT 60
           MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKT
Sbjct: 1   MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKT 60

Query: 61  VAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERD 120
           VAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERD
Sbjct: 61  VAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240

Query: 241 SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES 300
           SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES
Sbjct: 241 SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES 300

Query: 301 HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360
           HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME
Sbjct: 301 HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT 420
           KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT
Sbjct: 361 KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ
Sbjct: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLL 659
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLL 658

[Close]

BLAST of Csa4G105350 vs. NCBI nr
Match: gi|659111267|ref|XP_008455663.1| (PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo])

HSP 1 Score: 1227.6 bits (3175), Expect = 0.0e+00
Identity = 630/658 (95.74%), Postives = 640/658 (97.26%), Query Frame = 1


Query: 1   MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAVSSTRTWKVSYIENLQSKPKT 60
           MGPFPLLD AIEI PRCPIITSRSSYGRRSSHCHL +T VS+TR WKVSYIENLQSKPKT
Sbjct: 1   MGPFPLLDTAIEIFPRCPIITSRSSYGRRSSHCHLLVTTVSATRNWKVSYIENLQSKPKT 60

Query: 61  VAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEKERD 120
           V FSSRDNSNDHLTDLVNDADGF+TGRSEVLETGEDEILAVKKALLESQTRQ+AVEKERD
Sbjct: 61  VVFSSRDNSNDHLTDLVNDADGFTTGRSEVLETGEDEILAVKKALLESQTRQKAVEKERD 120

Query: 121 QLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLVLAK 180
           QLLERLARYEAKQKEYVATILHDKELA+SELE ARSLFNKKLEESVGEKFALESKLVLAK
Sbjct: 121 QLLERLARYEAKQKEYVATILHDKELAISELEAARSLFNKKLEESVGEKFALESKLVLAK 180

Query: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240
           QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML
Sbjct: 181 QDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEGSML 240

Query: 241 SFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINELES 300
           SFLEQSKIAIEKALDVAEKAS HAKKAMATFTDEVYPLD I SIQSENIKLKGV+NELES
Sbjct: 241 SFLEQSKIAIEKALDVAEKASVHAKKAMATFTDEVYPLDGITSIQSENIKLKGVVNELES 300

Query: 301 HLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360
           HLSLAR++V+NLKLELE ARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME
Sbjct: 301 HLSLARTDVDNLKLELENARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKLMME 360

Query: 361 KIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAGT 420
           KIKKDVADKKKAASK FK ELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEA T
Sbjct: 361 KIKKDVADKKKAASKAFKVELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASEAAT 420

Query: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRREIPQ 480
           KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLL+DGPRREIPQ
Sbjct: 421 KMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLTDGPRREIPQ 480

Query: 481 WMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHVIEK 540
           WMARRIGTIRPKFPPRKIDVTEIS SKFRSLDLPKLEEVWSIAQEKPKVGD LIEHVIEK
Sbjct: 481 WMARRIGTIRPKFPPRKIDVTEISASKFRSLDLPKLEEVWSIAQEKPKVGDALIEHVIEK 540

Query: 541 ETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600
           ETIEKKRKALERALQRKT QWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA
Sbjct: 541 ETIEKKRKALERALQRKTKQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLELAA 600

Query: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDLL 659
           KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGT EELKYCIEEFHSQDLL
Sbjct: 601 KASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTEEELKYCIEEFHSQDLL 658

[Close]

BLAST of Csa4G105350 vs. NCBI nr
Match: gi|743912428|ref|XP_011000093.1| (PREDICTED: uncharacterized protein LOC105107752 [Populus euphratica])

HSP 1 Score: 852.8 bits (2202), Expect = 4.1e-244
Identity = 435/627 (69.38%), Postives = 527/627 (84.05%), Query Frame = 1


Query: 32  HCHLRLTAVSSTRTWKVSYIENLQSKPKTVAFSSRDNSNDHLTDLVNDADGFSTGRSEVL 91
           H H  +   + TR  ++    N   K +TV FS+ D+ ND   ++VND DGF    +E L
Sbjct: 29  HHHSLIFPPARTRKRRLLSNGNWNRKSRTVVFSTADDFNDSSANMVNDDDGFMLRGTEDL 88

Query: 92  ETGEDEILAVKKALLESQTRQEAVEKERDQLLERLARYEAKQKEYVATILHDKELAVSEL 151
              E+E++A KKAL E++ RQEA+EKERDQLLE LA+ +AKQKE+VATIL DKE+A++EL
Sbjct: 89  VIEENELVATKKALSEARARQEAIEKERDQLLEELAQSQAKQKEHVATILRDKEVAITEL 148

Query: 152 EGARSLFNKKLEESVGEKFALESKLVLAKQDAIDLAVQVEKLAAIAFQQATSHILEDAQY 211
           E A+SLF+ KL+ESV EKF LESKLVLAKQDA++LAVQVEKLA IAFQQATSHILEDAQ 
Sbjct: 149 EAAKSLFHNKLQESVEEKFTLESKLVLAKQDAVELAVQVEKLAEIAFQQATSHILEDAQR 208

Query: 212 RVSVAETSAIETSYEIEKQIRDATEGSMLSFLEQSKIAIEKALDVAEKASAHAKKAMATF 271
           RVS AETSA E ++ IE+Q+R+ATEG++LS +EQSK AI+KALDVAEKA  +A +A+A F
Sbjct: 209 RVSAAETSAAEAAFHIEEQVRNATEGTILSIVEQSKDAIDKALDVAEKAGDYATRAVAVF 268

Query: 272 TDEVYPLDEIASIQSENIKLKGVINELESHLSLARSNVNNLKLELEQARAQATASEIRAK 331
           TD + P+DEIAS+QSENIKL+G++N+LES L + R+++  LK+ELEQ  AQA AS++RA+
Sbjct: 269 TDGINPVDEIASVQSENIKLQGIVNDLESQLLITRNDIVKLKVELEQVNAQAIASKLRAE 328

Query: 332 NAEKVLVEFQELSREKINQQEGEIKLMMEKIKKDVADKKKAASKVFKAELEGIKSAIQAA 391
           +AEK L+EFQE +RE   Q+E EI  ++EK+KKD A+KK+AASK FKAELE IK+AI+AA
Sbjct: 329 DAEKGLLEFQESNREMTIQREEEINSLLEKMKKDAAEKKEAASKAFKAELESIKAAIKAA 388

Query: 392 KETAHSKDSAYMRRCEALQRLLRASEAGTKMWQQRADMAESFLLKERTMGKDNEDAAYIV 451
           KETAHS++ AY+RRCEALQR LRASEA +KMW+ RA++AES L+KE    +++EDA YIV
Sbjct: 389 KETAHSRNEAYVRRCEALQRSLRASEAASKMWKHRAEIAESLLIKE----EEDEDAIYIV 448

Query: 452 NGGRIDLLTDDESQKWKLLSDGPRREIPQWMARRIGTIRPKFPPRKIDVTEISVSKFRSL 511
           NGGRIDLLTDD+SQKWKLLSDGPRRE PQWMARRI +IRPKFPPRKIDV+E   S FR L
Sbjct: 449 NGGRIDLLTDDDSQKWKLLSDGPRRETPQWMARRIRSIRPKFPPRKIDVSEALTSNFRPL 508

Query: 512 DLPKLEEVWSIAQEKPKVGDTLIEHVIEKETIEKKRKALERALQRKTIQWQRTPDQTKLE 571
           DLPK +EVWSIAQEK K  DTLIEHVIEKETIEKKRKALERALQRKTIQWQ+TP++TKLE
Sbjct: 509 DLPKPDEVWSIAQEKLKERDTLIEHVIEKETIEKKRKALERALQRKTIQWQKTPEETKLE 568

Query: 572 PGTGTGHEIVFQGFNWESWRRRWYLELAAKASDLSQSGITAVWLPPPTESVAPQGYMPSD 631
           PGTGTG EIVFQGFNWESWR++WYL+LA KA+DLS+ G+TAVWLPPPTESVAPQGYMPSD
Sbjct: 569 PGTGTGREIVFQGFNWESWRKQWYLDLAPKAADLSRCGVTAVWLPPPTESVAPQGYMPSD 628

Query: 632 LYNLNSSYGTVEELKYCIEEFHSQDLL 659
           LYNLNS+YG+VEELK+CIEE HSQDLL
Sbjct: 629 LYNLNSAYGSVEELKHCIEEMHSQDLL 651

[Close]

BLAST of Csa4G105350 vs. NCBI nr
Match: gi|645249726|ref|XP_008230873.1| (PREDICTED: uncharacterized protein LOC103330100 [Prunus mume])

HSP 1 Score: 851.3 bits (2198), Expect = 1.2e-243
Identity = 443/661 (67.02%), Postives = 529/661 (80.03%), Query Frame = 1


Query: 1   MGPFPLLDAAIEISPRCPIITSRSSYGRRSSHCHLRLTAV---SSTRTWKVSYIENLQSK 60
           MG   L D A+ I   CP   S S +    + C  R   +   +S R        +   K
Sbjct: 1   MGSISLPDMAVGIIHHCPTTYSGSHWKYDKNLCLGRRPPIFRTASKRKKNPFCKPHWLCK 60

Query: 61  PKTVAFSSRDNSNDHLTDLVNDADGFSTGRSEVLETGEDEILAVKKALLESQTRQEAVEK 120
            +T  FSS D+S D   D+V      ++GR+EVL   EDE++  +KAL E+Q RQEA+EK
Sbjct: 61  SRTRIFSSMDDSGDTFADVVE-----TSGRNEVLNIEEDELMTARKALSEAQARQEAIEK 120

Query: 121 ERDQLLERLARYEAKQKEYVATILHDKELAVSELEGARSLFNKKLEESVGEKFALESKLV 180
           ERDQLLE+LA  EAKQ+EY+ATILH+KELA++E+E A+SLF++KL+ESV EKF+LESKLV
Sbjct: 121 ERDQLLEKLACSEAKQQEYIATILHEKELAIAEVEAAKSLFDQKLQESVEEKFSLESKLV 180

Query: 181 LAKQDAIDLAVQVEKLAAIAFQQATSHILEDAQYRVSVAETSAIETSYEIEKQIRDATEG 240
           LAK DA++LAVQVEKLA IAFQQATSHILEDAQ RVS AET+A E +YE+EKQIRD TEG
Sbjct: 181 LAKNDAVELAVQVEKLAEIAFQQATSHILEDAQLRVSAAETAAAEAAYEMEKQIRDVTEG 240

Query: 241 SMLSFLEQSKIAIEKALDVAEKASAHAKKAMATFTDEVYPLDEIASIQSENIKLKGVINE 300
           S+LS +EQSK AIEKALDVAEKA  HA KA++ FT+ + PLDE+ASIQS+NI L+GV+N+
Sbjct: 241 SILSIVEQSKYAIEKALDVAEKAGEHATKAVSEFTEGMNPLDELASIQSKNIMLQGVVND 300

Query: 301 LESHLSLARSNVNNLKLELEQARAQATASEIRAKNAEKVLVEFQELSREKINQQEGEIKL 360
           LES L L RS+V+ LKLELE+A A A A E+RAK+AEK L+EFQE S++   Q+E EI  
Sbjct: 301 LESQLLLTRSDVDRLKLELEKAHAHANAFELRAKDAEKALLEFQESSKKNTLQKEEEIMS 360

Query: 361 MMEKIKKDVADKKKAASKVFKAELEGIKSAIQAAKETAHSKDSAYMRRCEALQRLLRASE 420
           ++EK+KKD +++KK +SK FKAEL+ I+ AI AAKE AHSKD AY+RRCEALQR L+ASE
Sbjct: 361 LIEKMKKDSSERKKNSSKAFKAELQSIRDAIGAAKEMAHSKDDAYLRRCEALQRSLKASE 420

Query: 421 AGTKMWQQRADMAESFLLKERTMGKDNEDAAYIVNGGRIDLLTDDESQKWKLLSDGPRRE 480
           A TKMW+QRA+MAES L KER +G+ +ED+ Y+VNGGRIDLLTDD+SQKWKLLSDGPRRE
Sbjct: 421 ATTKMWRQRAEMAESILCKERPLGEGDEDSIYVVNGGRIDLLTDDDSQKWKLLSDGPRRE 480

Query: 481 IPQWMARRIGTIRPKFPPRKIDVTEISVSKFRSLDLPKLEEVWSIAQEKPKVGDTLIEHV 540
           IPQWMAR+I TIRP+FPPRKIDV E   SKFRSLDLPK  EVWSIAQEKPK GD LIEHV
Sbjct: 481 IPQWMARKIRTIRPRFPPRKIDVAEALSSKFRSLDLPKPNEVWSIAQEKPKEGDILIEHV 540

Query: 541 IEKETIEKKRKALERALQRKTIQWQRTPDQTKLEPGTGTGHEIVFQGFNWESWRRRWYLE 600
           IEKETIEKKRKALE  LQ KTIQWQ+TP+QT LE GTGTG EIVFQGFNWESWR++WYL+
Sbjct: 541 IEKETIEKKRKALEHVLQGKTIQWQKTPEQTNLESGTGTGREIVFQGFNWESWRKQWYLD 600

Query: 601 LAAKASDLSQSGITAVWLPPPTESVAPQGYMPSDLYNLNSSYGTVEELKYCIEEFHSQDL 659
           LA KA+DLS+ G+T+VW PPPTESVAPQGYMPSDLYNLNSSYG+VEELK+CIEE HS  L
Sbjct: 601 LAPKAADLSKIGVTSVWFPPPTESVAPQGYMPSDLYNLNSSYGSVEELKHCIEEMHSHGL 656

The following BLAST results are available for this feature:

BLAST of Csa4G105350 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AMY3_ARATH	1.8e-26	39.20	Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana GN=AMY3 PE=2 SV=1	[more]
AMY3A_ORYSJ	4.8e-19	53.66	Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2	[more]
AMY3D_ORYSJ	8.2e-19	53.75	Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica GN=AMY1.3 PE=2 SV=2	[more]
AMY3E_ORYSJ	1.1e-18	53.66	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica GN=AMY1.4 PE=2 SV=1	[more]
AMY3B_ORYSJ	1.1e-18	46.23	Alpha-amylase isozyme 3B OS=Oryza sativa subsp. japonica GN=AMY1.6 PE=2 SV=1	[more]

BLAST of Csa4G105350 vs. TrEMBL


Match Name	E-value	Identity	Description
B9IK70_POPTR	4.1e-243	69.06	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s12870g PE=4 SV=2	[more]
B9SJF1_RICCO	1.6e-242	68.98	Alpha-amylase, putative OS=Ricinus communis GN=RCOM_0526780 PE=4 SV=1	[more]
M5WYH2_PRUPE	2.0e-242	67.33	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000951mg PE=4 SV=1	[more]
W9QIQ2_9ROSA	1.4e-238	71.24	Alpha-amylase isozyme 2A OS=Morus notabilis GN=L484_021018 PE=4 SV=1	[more]
A0A0A0R208_MORAL	4.0e-238	65.76	Alpha-amylase OS=Morus alba var. multicaulis PE=2 SV=1	[more]

BLAST of Csa4G105350 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G69830.1	1.0e-27	39.20	alpha-amylase-like 3	[more]
AT1G76130.1	1.9e-18	46.91	alpha-amylase-like 2	[more]
AT4G25000.1	6.9e-16	46.91	alpha-amylase-like	[more]
AT4G14760.1	1.4e-08	22.85	kinase interacting (KIP1-like) family protein	[more]
AT2G32240.1	4.0e-08	22.58	FUNCTIONS IN: molecular_function unknown	[more]

BLAST of Csa4G105350 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700198531\|gb\|KGN53689.1\|	0.0e+00	100.00	hypothetical protein Csa_4G105350 [Cucumis sativus]	[more]
gi\|449438801\|ref\|XP_004137176.1\|	0.0e+00	100.00	PREDICTED: uncharacterized protein LOC101217339 [Cucumis sativus]	[more]
gi\|659111267\|ref\|XP_008455663.1\|	0.0e+00	95.74	PREDICTED: uncharacterized protein LOC103495777 [Cucumis melo]	[more]
gi\|743912428\|ref\|XP_011000093.1\|	4.1e-244	69.38	PREDICTED: uncharacterized protein LOC105107752 [Populus euphratica]	[more]
gi\|645249726\|ref\|XP_008230873.1\|	1.2e-243	67.02	PREDICTED: uncharacterized protein LOC103330100 [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006047	Glyco_hydro_13_cat_dom
IPR013781	Glycoside hydrolase, catalytic domain
IPR015902	Glycoside hydrolase, family 13
IPR017853	Glycoside_hydrolase_SF

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0033910	glucan 1,4-alpha-maltotetraohydrolase activity
molecular_function	GO:0003824	catalytic activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU124204	cucumber EST collection version 3.0	transcribed_cluster
CU149575	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa4G105350.1	Csa4G105350.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU149575	CU149575	transcribed_cluster
CU124204	CU124204	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 608..658 score: 1.
IPR013781	Glycoside hydrolase, catalytic domain	GENE3D	G3DSA:3.20.20.80		coord: 577..657 score: 2.0
IPR015902	Glycoside hydrolase, family 13	PANTHER	PTHR10357	ALPHA-AMYLASE	coord: 547..659 score: 1.1E-73coord: 267..439 score: 1.1
IPR017853	Glycoside hydrolase superfamily	unknown	SSF51445	(Trans)glycosidases	coord: 579..659 score: 2.78
None	No IPR available	unknown	Coil	Coil	coord: 295..336 score: -coord: 105..139 score: -coord: 533..560 scor
None	No IPR available	PANTHER	PTHR10357:SF157	SUBFAMILY NOT NAMED	coord: 267..439 score: 1.1E-73coord: 547..659 score: 1.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa4G105350	MELO3C019035	Melon (DHL92) v3.5.1	cumeB317
Csa4G105350	CSPI04G09100	Wild cucumber (PI 183967)	cpicuB182
Csa4G105350	Cucsa.397910	Cucumber (Gy14) v1	cgycuB556

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa4G105350	Cucumber (Gy14) v2	cgybcuB165
Csa4G105350	Melon (DHL92) v3.6.1	cumedB288
Csa4G105350	Melon (DHL92) v3.6.1	cumedB303
Csa4G105350	Melon (DHL92) v3.5.1	cumeB303