Csa4G099230 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa4G099230
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Clathrin assembly protein, putative; contains IPR008942 (ENTH/VHS), IPR011417 (AP180 N-terminal homology (ANTH) domain)
Location	Chr4 : 6489370 .. 6490733 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGACCAAAACCAACTTTTGAAAGGCCAAATATTAAAATACATCCAATTGAAAATTAATAATAAATAAAAGAAATTGTACTCATTTCCAAATCAAATCAATTTCTCCATAAGTAAAAAAAAATTATTCTCTGTTTGTTAGATGTTGATTTCTCTCAGCCTTTCAATGGTGAACACAAAAAAACTGAGTTCCCTAATTGGACTCATCAAAGACAAAGCCTCTCAAAGCAAAGCCGCCCTTCTGGCAAAGCCAAACATACTGTCCTTTCAACTCGCTCTCCTCCGAGCCACCACTCACGACCTCCACGCGCCACCCAGCGACAAGCACCTCTCTGCCCTTCTCTCTCTTGGCAAAACCTCACGCGCCACCGCCGCTCCTGCCGTTGAAGTCTTAATGGACCGCCTCCAAACCACCCATAACTCCGCCGTCGCTCTCAAGTGCCTTATCGCCGTCCATCACATCTTCAAGGATGGCGACTTTATTCTTCAAGACCAGCTCTCTGTTTTTCCCTTCACCGGTGGTAGAAACTACCTCAAACTCTCTGATTTCCGCGACAGTTCCAATCCCATCTCTTGGGACCTTTCCTCTTGGGTCCGATGGTACGCTCAGTACATCGAAACTGTTTTGTCTATTTCCCGGATTTTGGGGTTTTTTGTGGGTTCTTCAAGGTCCAACGAAGAGAAGGAGAGAAAAACAGAACAGATTTCAGGGATTTTGAACTCCGATTTGCTTAAAGAGACCGAATCTTTGGTGGGTTTAATCGAAGAAATTTCGAAAATGCCTCACTGTTTGCATCTGAATAGAAACAGATTGGTGGATAAGATCTACAGCTTTGTCGGTGATGATTATTTGTCGGCCATGAAGGAAATTTCAATCCGAGTTACCGAGTTTCACCACCGGCTCGGTTGGCTCAGTTTCGCCGAATCGGTCGAGTTGGTTTGCGCGTTGAAACGGCTCGAGGATTGCAAAGAAAAGCAATCCATGGGAATTTTTGCAAAGTACGAAGTTCTGATAGATGGACTTTGGGGTTCCATCCGTTCCATCCAAGAGACCAAGAATTTGACTGGGGAATCGAAGGAACATCGAGAGGGCGGTAAATTGTGCAAGACGAAGAGGAGGGTCAGCGACTCGGGCCGGTTTATGGAGCGGCCTAATGCTAGTTCTTACCGTGACCTTCTTAGATTCGGGTCGGAACGGTTCGTTTTAACCTACGACGGTTTCCAGTAATACCTATACCGGAATCGTAGTTACTACTTGCTACCAAAATATAATTATGGAAAAATGAGGTATGTAGCATCCATTTAAGTTTCATTTTGATATATAGTAACCTTCTGAATTTTAGCCAAATTTATGTATGTCACGTG

mRNA sequence

ATGTTGATTTCTCTCAGCCTTTCAATGGTGAACACAAAAAAACTGAGTTCCCTAATTGGACTCATCAAAGACAAAGCCTCTCAAAGCAAAGCCGCCCTTCTGGCAAAGCCAAACATACTGTCCTTTCAACTCGCTCTCCTCCGAGCCACCACTCACGACCTCCACGCGCCACCCAGCGACAAGCACCTCTCTGCCCTTCTCTCTCTTGGCAAAACCTCACGCGCCACCGCCGCTCCTGCCGTTGAAGTCTTAATGGACCGCCTCCAAACCACCCATAACTCCGCCGTCGCTCTCAAGTGCCTTATCGCCGTCCATCACATCTTCAAGGATGGCGACTTTATTCTTCAAGACCAGCTCTCTGTTTTTCCCTTCACCGGTGGTAGAAACTACCTCAAACTCTCTGATTTCCGCGACAGTTCCAATCCCATCTCTTGGGACCTTTCCTCTTGGGTCCGATGGTACGCTCAGTACATCGAAACTGTTTTGTCTATTTCCCGGATTTTGGGGTTTTTTGTGGGTTCTTCAAGGTCCAACGAAGAGAAGGAGAGAAAAACAGAACAGATTTCAGGGATTTTGAACTCCGATTTGCTTAAAGAGACCGAATCTTTGGTGGGTTTAATCGAAGAAATTTCGAAAATGCCTCACTGTTTGCATCTGAATAGAAACAGATTGGTGGATAAGATCTACAGCTTTGTCGGTGATGATTATTTGTCGGCCATGAAGGAAATTTCAATCCGAGTTACCGAGTTTCACCACCGGCTCGGTTGGCTCAGTTTCGCCGAATCGGTCGAGTTGGTTTGCGCGTTGAAACGGCTCGAGGATTGCAAAGAAAAGCAATCCATGGGAATTTTTGCAAAGTACGAAGTTCTGATAGATGGACTTTGGGGTTCCATCCGTTCCATCCAAGAGACCAAGAATTTGACTGGGGAATCGAAGGAACATCGAGAGGGCGGTAAATTGTGCAAGACGAAGAGGAGGGTCAGCGACTCGGGCCGGTTTATGGAGCGGCCTAATGCTAGTTCTTACCGTGACCTTCTTAGATTCGGGTCGGAACGGTTCGTTTTAACCTACGACGGTTTCCAGTAA

Coding sequence (CDS)

ATGTTGATTTCTCTCAGCCTTTCAATGGTGAACACAAAAAAACTGAGTTCCCTAATTGGACTCATCAAAGACAAAGCCTCTCAAAGCAAAGCCGCCCTTCTGGCAAAGCCAAACATACTGTCCTTTCAACTCGCTCTCCTCCGAGCCACCACTCACGACCTCCACGCGCCACCCAGCGACAAGCACCTCTCTGCCCTTCTCTCTCTTGGCAAAACCTCACGCGCCACCGCCGCTCCTGCCGTTGAAGTCTTAATGGACCGCCTCCAAACCACCCATAACTCCGCCGTCGCTCTCAAGTGCCTTATCGCCGTCCATCACATCTTCAAGGATGGCGACTTTATTCTTCAAGACCAGCTCTCTGTTTTTCCCTTCACCGGTGGTAGAAACTACCTCAAACTCTCTGATTTCCGCGACAGTTCCAATCCCATCTCTTGGGACCTTTCCTCTTGGGTCCGATGGTACGCTCAGTACATCGAAACTGTTTTGTCTATTTCCCGGATTTTGGGGTTTTTTGTGGGTTCTTCAAGGTCCAACGAAGAGAAGGAGAGAAAAACAGAACAGATTTCAGGGATTTTGAACTCCGATTTGCTTAAAGAGACCGAATCTTTGGTGGGTTTAATCGAAGAAATTTCGAAAATGCCTCACTGTTTGCATCTGAATAGAAACAGATTGGTGGATAAGATCTACAGCTTTGTCGGTGATGATTATTTGTCGGCCATGAAGGAAATTTCAATCCGAGTTACCGAGTTTCACCACCGGCTCGGTTGGCTCAGTTTCGCCGAATCGGTCGAGTTGGTTTGCGCGTTGAAACGGCTCGAGGATTGCAAAGAAAAGCAATCCATGGGAATTTTTGCAAAGTACGAAGTTCTGATAGATGGACTTTGGGGTTCCATCCGTTCCATCCAAGAGACCAAGAATTTGACTGGGGAATCGAAGGAACATCGAGAGGGCGGTAAATTGTGCAAGACGAAGAGGAGGGTCAGCGACTCGGGCCGGTTTATGGAGCGGCCTAATGCTAGTTCTTACCGTGACCTTCTTAGATTCGGGTCGGAACGGTTCGTTTTAACCTACGACGGTTTCCAGTAA

Protein sequence

MLISLSLSMVNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSLGKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQISGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTEFHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQETKNLTGESKEHREGGKLCKTKRRVSDSGRFMERPNASSYRDLLRFGSERFVLTYDGFQ*

Homology

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BLAST of Csa4G099230 vs. Swiss-Prot
Match: CAP16_ARATH (Putative clathrin assembly protein At4g40080 OS=Arabidopsis thaliana GN=At4g40080 PE=2 SV=2)

HSP 1 Score: 307.4 bits (786), Expect = 2.1e-82
Identity = 173/348 (49.71%), Postives = 227/348 (65.23%), Query Frame = 1


Query: 16  SSLIGLIKDKASQSKAALLA---KPNILSFQLALLRATTHDLHAPPSDKHLSALLSLGKT 75
           + LIG IKDKASQSKAAL++   K   LSF L++LRATTHD   PP ++HL+ +LS G  
Sbjct: 8   ADLIGRIKDKASQSKAALVSSNTKSKTLSFHLSVLRATTHDPSTPPGNRHLAVILSAGTG 67

Query: 76  SRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLK 135
           SRATA+ AVE +M+RL TT ++ VALK LI +HHI K G FILQDQLSVFP +GGRNYLK
Sbjct: 68  SRATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSVFPASGGRNYLK 127

Query: 136 LSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQISGIL 195
           LS FRD  +P+ W+LSSWVRWYA Y+E +LS SRI+GFF+ S+ S   KE   E +S + 
Sbjct: 128 LSAFRDEKSPLMWELSSWVRWYALYLEHLLSTSRIMGFFISSTSSTIHKEEYEEMVSSLT 187

Query: 196 NSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTEFHH 255
           NSDLL+E ++LVGL+EE  K+P         L DKI   VG+DY+S++ E+  R  EF  
Sbjct: 188 NSDLLREIDALVGLLEEACKIPDLPFSGGKSLADKITQLVGEDYVSSINELYTRFNEFKE 247

Query: 256 RLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYE-VLIDGLWGSIRSIQETKNLTGES 315
           R   LSF +++ELVCALKRLE CKE+ S      ++   IDG WG    + E K + G  
Sbjct: 248 RSNTLSFGDTIELVCALKRLESCKERLSEICHGNWKRGWIDGFWG---LVLEVKGIIGNL 307

Query: 316 KEHREGGKLCKT------KRRVSDSGRFMERPNASSYRDLLRFGSERF 354
           +++   G++ K+      + +  +S RF +R     Y + +RF S RF
Sbjct: 308 EDNY--GQIEKSIVGFGKRDKGYESARFTDR-LIIGYSNPVRFSSGRF 349

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BLAST of Csa4G099230 vs. Swiss-Prot
Match: CAP18_ARATH (Putative clathrin assembly protein At5g65370 OS=Arabidopsis thaliana GN=At5g65370 PE=3 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 6.2e-34
Identity = 94/274 (34.31%), Postives = 154/274 (56.20%), Query Frame = 1


Query: 14  KLSSLIGLIKDKASQSK---AALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSLG 73
           KL++L G++KD+ASQ K     L +  N  +  LALL+AT+H  + PPSDK+++ L S  
Sbjct: 3   KLATLNGILKDEASQMKLNVVHLCSSVNAKTIDLALLKATSHTSNNPPSDKYVTFLQSTI 62

Query: 74  KTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFK-----DGDFILQDQLS--VFP 133
            T        V+ ++ RL+ T +  VA KCLI +H + K     +G+  L++ ++     
Sbjct: 63  DT--CYGPDTVDAILHRLRVTTDVCVAAKCLILLHKMVKSESGYNGEDSLRNNINHRTLI 122

Query: 134 FTGGRNYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKER 193
           +T G + LKL+D   +S+  + +L+ WV+WY QY++  LSI+ +LG        NE+K  
Sbjct: 123 YTQGGSNLKLNDLNVNSSRFTRELTPWVQWYKQYLDCYLSIAEVLGITPNIKEKNEDKRL 182

Query: 194 KTEQISGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEI 253
           +T+++S      +LK+ + LV L E IS  P       N++V ++   +  DY SA++ +
Sbjct: 183 ETQRVSSYPMDCILKQIDFLVELFEHISDRPKAPQSKLNKIVIEMTELMVQDYFSAIRLM 242

Query: 254 SIRVTEFHHRLGWLSFAESVELVCALKRLEDCKE 278
            IR  E + R+     A+  ELV  L++LE+CKE
Sbjct: 243 RIRFEELNVRV-----AKPNELVPVLEKLENCKE 269

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BLAST of Csa4G099230 vs. Swiss-Prot
Match: CAP17_ARATH (Putative clathrin assembly protein At5g10410 OS=Arabidopsis thaliana GN=At5g10410 PE=2 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 5.5e-30
Identity = 93/303 (30.69%), Postives = 153/303 (50.50%), Query Frame = 1


Query: 18  LIGLIKDKASQSKAALL---AKPNILSFQLALLRATTHDLHAPPSDKHLSALLSLGKTSR 77
           +IG  KDKAS  KA L+       +    LALL++TT   + PP+  ++SA++S   +  
Sbjct: 8   IIGKFKDKASIGKARLVHSFGSTAVKYIHLALLKSTTRTPNKPPNSDYVSAVISYSNSRY 67

Query: 78  ATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLKLS 137
           A AA      + RL+ T N+ VA K LI +H + K      +D+        GRN LKL+
Sbjct: 68  APAA--FSAALWRLRVTKNAIVATKSLIVIHKLIKSS----RDKFEGLGH--GRNNLKLN 127

Query: 138 DFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQISGILNS 197
           +F D S+ ++ +LS W+RWY QY++ +  + ++LG F     + ++K  + +++S     
Sbjct: 128 EFSDKSSNLTLELSQWIRWYGQYLDRLSWVPKVLGSFPNLLVNPKDKVEEKDRVSSYQTG 187

Query: 198 DLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTEFHHRL 257
            ++++T+SLV   E I   P    + +N++VD+I   V +DY   ++ + +R+     RL
Sbjct: 188 YIIRQTDSLVSFFEHICTRPEIPPMFQNKIVDEIRELVIEDYFKIVRLVMVRLQVLFERL 247

Query: 258 --------GWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQ---E 307
                   G L   +   L   L RL +CKE  S G+F +   L D  W  +  ++   E
Sbjct: 248 IKPGVKPIGDLGLNDFSLL---LVRLVECKESLS-GLFWRCRRLADDFWCLVEMLKAETE 298

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BLAST of Csa4G099230 vs. Swiss-Prot
Match: CAP8_ARATH (Putative clathrin assembly protein At2g01600 OS=Arabidopsis thaliana GN=At2g01600 PE=2 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 9.6e-19
Identity = 61/193 (31.61%), Postives = 99/193 (51.30%), Query Frame = 1


Query: 20  GLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSLGKTSRATA-- 79
           G +KD  S     +          +A+++AT H +  PP D+HL  + +    +RA A  
Sbjct: 12  GALKD--STKVGLVRVNSEYADLDVAIVKATNH-VECPPKDRHLRKIFAATSVTRARADV 71

Query: 80  APAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLKLSDFR 139
           A  +  L  RL  T N  VALK LI +H + ++GD   +++L  F   G    L+LS+F+
Sbjct: 72  AYCIHALSRRLHKTRNWTVALKTLIVIHRLLREGDPTFREELLNFSQRG--RILQLSNFK 131

Query: 140 DSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSR---SNEEKERKTEQISGILNS 199
           D S+PI+WD S+WVR YA ++E  L   R+L +   + R   SN  +++   +   +   
Sbjct: 132 DDSSPIAWDCSAWVRTYALFLEERLECFRVLKYDTEAERLPKSNPGQDKGYSRTRDLDGE 191

Query: 200 DLLKETESLVGLI 208
           +LL++  +L  L+
Sbjct: 192 ELLEQLPALQQLL 199

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BLAST of Csa4G099230 vs. Swiss-Prot
Match: CAP7_ARATH (Putative clathrin assembly protein At5g57200 OS=Arabidopsis thaliana GN=At5g57200 PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 6.3e-18
Identity = 60/197 (30.46%), Postives = 102/197 (51.78%), Query Frame = 1


Query: 20  GLIKDKASQSKAALLAKPN--ILSFQLALLRATTHDLHAPPSDKHLSALLSLGKT--SRA 79
           G +KD  +      LAK N       +A+++AT H + +PP ++H+  + S       RA
Sbjct: 12  GALKDTTTVG----LAKVNSEFKDLDIAIVKATNH-VESPPKERHVRKIFSATSVIQPRA 71

Query: 80  TAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLKLSD 139
             A  +  L  RL  T N  VA+K LI +H   ++GD   +++L    ++  R+ L++S+
Sbjct: 72  DVAYCIHALSKRLSKTRNWVVAMKVLIVIHRTLREGDPTFREEL--LNYSHRRHILRISN 131

Query: 140 FRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSR-----SNEEKERKTEQISG 199
           F+D ++P++WD S+WVR YA ++E  L   R+L + + + R         K  +T  +SG
Sbjct: 132 FKDDTSPLAWDCSAWVRTYALFLEERLECYRVLKYDIEAERLPKASGAASKTHRTRMLSG 191

Query: 200 ILNSDLLKETESLVGLI 208
               DLL++  +L  L+
Sbjct: 192 ---EDLLEQLPALQQLL 198

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BLAST of Csa4G099230 vs. TrEMBL
Match: A0A0A0KXU4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G099230 PE=4 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 9.2e-202
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 1


Query: 1   MLISLSLSMVNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSD 60
           MLISLSLSMVNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSD
Sbjct: 1   MLISLSLSMVNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSD 60

Query: 61  KHLSALLSLGKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLS 120
           KHLSALLSLGKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLS
Sbjct: 61  KHLSALLSLGKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLS 120

Query: 121 VFPFTGGRNYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEE 180
           VFPFTGGRNYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEE
Sbjct: 121 VFPFTGGRNYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEE 180

Query: 181 KERKTEQISGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAM 240
           KERKTEQISGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAM
Sbjct: 181 KERKTEQISGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAM 240

Query: 241 KEISIRVTEFHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRS 300
           KEISIRVTEFHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRS
Sbjct: 241 KEISIRVTEFHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRS 300

Query: 301 IQETKNLTGESKEHREGGKLCKTKRRVSDSGRFMERPNASSYRDLLRFGSERFVLTYDGF 360
           IQETKNLTGESKEHREGGKLCKTKRRVSDSGRFMERPNASSYRDLLRFGSERFVLTYDGF
Sbjct: 301 IQETKNLTGESKEHREGGKLCKTKRRVSDSGRFMERPNASSYRDLLRFGSERFVLTYDGF 360

Query: 361 Q 362
           Q
Sbjct: 361 Q 361

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BLAST of Csa4G099230 vs. TrEMBL
Match: A0A061DPV2_THECC (ENTH/ANTH/VHS superfamily protein, putative OS=Theobroma cacao GN=TCM_001021 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 5.4e-101
Identity = 196/342 (57.31%), Postives = 256/342 (74.85%), Query Frame = 1


Query: 15  LSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSLGKTSR 74
           L  LIG+IKDKASQSKAALL+ P  LS  LALLRATTHD   PP  +HL+ALLS G +SR
Sbjct: 7   LRDLIGIIKDKASQSKAALLSNPKTLSLHLALLRATTHDPFTPPDPRHLAALLSFGHSSR 66

Query: 75  ATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLKLS 134
           A AA A+E LMDRLQTT +++VA+KCL  +HHI K G FILQDQLSVFP TGGRNYLKLS
Sbjct: 67  AIAATAIEALMDRLQTTRDASVAIKCLFTIHHIIKRGSFILQDQLSVFPATGGRNYLKLS 126

Query: 135 DFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQISGILNS 194
           +FRD++ P++W+LSSWVRWYA Y+E++LS SRILGFF+ S+ S+ + +++ E++S ++NS
Sbjct: 127 NFRDNTTPLTWELSSWVRWYALYLESLLSTSRILGFFLCSTSSSVDIDKEEEKVSSLINS 186

Query: 195 DLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTEFHHRL 254
           +LL+E  SLV L+E+ISK P+ LH N N LV++I   VG+DYLS++ E+SIRV E   RL
Sbjct: 187 ELLREINSLVNLLEQISKSPNSLHANGNILVEEIQGLVGEDYLSSINEVSIRVGEVRERL 246

Query: 255 GWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQETKNLTGESKEH 314
             LSF +SVE VCALKRLEDCKE+ S+ +  + +VLID +WG   SI E K+  G SK +
Sbjct: 247 SSLSFVDSVEWVCALKRLEDCKER-SLALSQRKKVLIDAVWG---SISEIKDQVGSSKVY 306

Query: 315 REGGKLCK--TKRRVSDSGRFMERPNASSYRDLLRFGSERFV 355
           RE G+L    ++ + S+S RF ER     + D ++F S RF+
Sbjct: 307 REEGRLLTMGSRNKASESARFGER--VLKHGDSVKFSSGRFL 342

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BLAST of Csa4G099230 vs. TrEMBL
Match: V4RXC7_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10025941mg PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 8.6e-99
Identity = 189/346 (54.62%), Postives = 256/346 (73.99%), Query Frame = 1


Query: 10  VNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSL 69
           +N  +L++L+G+IKDK SQSKAA+++KP  L+  L+LLRATTHD   PP  K L+ LLS 
Sbjct: 1   MNMGRLANLMGIIKDKVSQSKAAIISKPKTLTLHLSLLRATTHDPSTPPDPKRLTTLLSF 60

Query: 70  GKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRN 129
           G +SRATAA  +E LMDRLQTTH+++VA+K LIAVHHI K G FILQDQLSV+P  GGRN
Sbjct: 61  GHSSRATAAAVIEALMDRLQTTHDASVAIKSLIAVHHIVKHGSFILQDQLSVYPSAGGRN 120

Query: 130 YLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQIS 189
           YLKLS+FRD++ P++W+LSSWVRWYA Y+E +LS SR+LGFF+ SS S+ E +++ E++S
Sbjct: 121 YLKLSNFRDNTTPLTWELSSWVRWYALYLEHLLSTSRVLGFFLSSSSSSVEMDKEEEKVS 180

Query: 190 GILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTE 249
            ++N DLLKE +SL+ L+E++ K P CLH+  N LVD I   VG+DYLSA+ E+SIRV+E
Sbjct: 181 ALVNIDLLKEVDSLLSLLEQMCKTPDCLHVRGNPLVDDIMGLVGEDYLSAINEVSIRVSE 240

Query: 250 FHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQETKNLTG 309
           F++RLG LS  +SVEL CALKRLEDCKE+ S+ +  +  VLI+  WG I ++   K+   
Sbjct: 241 FNNRLGCLSLGDSVELACALKRLEDCKERLSV-LSHRKRVLIEAFWGLITAL---KDKVA 300

Query: 310 ESKEHREGGKLCKTKRR--VSDSGRFMERPNASSYRDLLRFGSERF 354
           + + +R+   +  T RR   S+S RF +R  +  Y D +RF S RF
Sbjct: 301 KERAYRDERMIVSTGRRDKASESARFGDR-LSRRYGDSVRFSSARF 341

[Close]

BLAST of Csa4G099230 vs. TrEMBL
Match: A0A067EZX7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g018185mg PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 8.6e-99
Identity = 189/346 (54.62%), Postives = 256/346 (73.99%), Query Frame = 1


Query: 10  VNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSL 69
           +N  +L++L+G+IKDK SQSKAA+++KP  L+  L+LLRATTHD   PP  K L+ LLS 
Sbjct: 1   MNMGRLANLMGIIKDKVSQSKAAIISKPKTLTLHLSLLRATTHDPSTPPDPKRLTTLLSF 60

Query: 70  GKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRN 129
           G +SRATAA  +E LMDRLQTTH+++VA+K LIAVHHI K G FILQDQLSV+P  GGRN
Sbjct: 61  GHSSRATAAAVIEALMDRLQTTHDASVAIKSLIAVHHIVKHGSFILQDQLSVYPSAGGRN 120

Query: 130 YLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQIS 189
           YLKLS+FRD++ P++W+LSSWVRWYA Y+E +LS SR+LGFF+ SS S+ E +++ E++S
Sbjct: 121 YLKLSNFRDNTTPLTWELSSWVRWYALYLEHLLSTSRVLGFFLSSSSSSVEMDKEEEKVS 180

Query: 190 GILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTE 249
            ++N DLLKE +SL+ L+E++ K P CLH+  N LVD I   VG+DYLSA+ E+SIRV+E
Sbjct: 181 ALVNIDLLKEVDSLLSLLEQMCKTPDCLHVRGNPLVDDIMGLVGEDYLSAINEVSIRVSE 240

Query: 250 FHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQETKNLTG 309
           F++RLG LS  +SVEL CALKRLEDCKE+ S+ +  +  VLI+  WG I ++   K+   
Sbjct: 241 FNNRLGCLSLGDSVELACALKRLEDCKERLSV-LSHRKRVLIEAFWGLITAL---KDKVA 300

Query: 310 ESKEHREGGKLCKTKRR--VSDSGRFMERPNASSYRDLLRFGSERF 354
           + + +R+   +  T RR   S+S RF +R  +  Y D +RF S RF
Sbjct: 301 KERAYRDERMIVSTGRRDKASESARFGDR-LSRRYGDSVRFSSARF 341

[Close]

BLAST of Csa4G099230 vs. TrEMBL
Match: A0A0B0NU88_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_23111 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.3e-96
Identity = 192/349 (55.01%), Postives = 252/349 (72.21%), Query Frame = 1


Query: 9   MVNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLS 68
           M   K    LIG+IKDKASQSKAAL++ P  LS  LALLRATTHD  +PP   HL+ LLS
Sbjct: 1   MGRVKVFRDLIGIIKDKASQSKAALISTPRTLSLHLALLRATTHDPFSPPDPTHLATLLS 60

Query: 69  LGKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGR 128
            G  SRATA+ AV+ +MDRLQTT +++VA+KCLI VHHI K G FILQDQLSV+P TGGR
Sbjct: 61  FGHCSRATASTAVDAIMDRLQTTRDASVAIKCLITVHHIIKRGSFILQDQLSVYPSTGGR 120

Query: 129 NYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQI 188
           NYLKLS+FRD + P++W+LSSWVRWYA Y+E +LS SRILGFF+ S+ S+ +K+R+ +++
Sbjct: 121 NYLKLSNFRDDTTPLTWELSSWVRWYALYLENLLSTSRILGFFLCSTSSSVDKDREEDRV 180

Query: 189 SGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVT 248
           S ++N++LLKE  SL  LIE+I+K P   + N N LVD +   VG+DYLS++ EISIRV+
Sbjct: 181 SSLINTELLKEINSLGNLIEQIAKKPDSSNSNGNVLVDAVLGLVGEDYLSSINEISIRVS 240

Query: 249 EFHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQETKNLT 308
           EF  RL  L F +SVELVCAL+RLE+CKE+ S  +  + +V+I+ +WG   SI E K+  
Sbjct: 241 EFKERLDCLGFVDSVELVCALRRLEECKERLST-LSQRKKVMIESVWG---SINEVKDQI 300

Query: 309 GESKEHREG-GKLCKTKRR--VSDSGRFMERPNASSYRDLLRFGSERFV 355
           G SK ++E  G+L    RR  VS+S RF ER       + ++F S RF+
Sbjct: 301 GNSKAYKEDEGRLLMMGRRNKVSESARFGERVVMKHSGNSVKFSSGRFL 345

[Close]

BLAST of Csa4G099230 vs. TAIR10
Match: AT4G40080.1 (AT4G40080.1 ENTH/ANTH/VHS superfamily protein)

HSP 1 Score: 307.4 bits (786), Expect = 1.2e-83
Identity = 173/348 (49.71%), Postives = 227/348 (65.23%), Query Frame = 1


Query: 16  SSLIGLIKDKASQSKAALLA---KPNILSFQLALLRATTHDLHAPPSDKHLSALLSLGKT 75
           + LIG IKDKASQSKAAL++   K   LSF L++LRATTHD   PP ++HL+ +LS G  
Sbjct: 8   ADLIGRIKDKASQSKAALVSSNTKSKTLSFHLSVLRATTHDPSTPPGNRHLAVILSAGTG 67

Query: 76  SRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLK 135
           SRATA+ AVE +M+RL TT ++ VALK LI +HHI K G FILQDQLSVFP +GGRNYLK
Sbjct: 68  SRATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSVFPASGGRNYLK 127

Query: 136 LSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQISGIL 195
           LS FRD  +P+ W+LSSWVRWYA Y+E +LS SRI+GFF+ S+ S   KE   E +S + 
Sbjct: 128 LSAFRDEKSPLMWELSSWVRWYALYLEHLLSTSRIMGFFISSTSSTIHKEEYEEMVSSLT 187

Query: 196 NSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTEFHH 255
           NSDLL+E ++LVGL+EE  K+P         L DKI   VG+DY+S++ E+  R  EF  
Sbjct: 188 NSDLLREIDALVGLLEEACKIPDLPFSGGKSLADKITQLVGEDYVSSINELYTRFNEFKE 247

Query: 256 RLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYE-VLIDGLWGSIRSIQETKNLTGES 315
           R   LSF +++ELVCALKRLE CKE+ S      ++   IDG WG    + E K + G  
Sbjct: 248 RSNTLSFGDTIELVCALKRLESCKERLSEICHGNWKRGWIDGFWG---LVLEVKGIIGNL 307

Query: 316 KEHREGGKLCKT------KRRVSDSGRFMERPNASSYRDLLRFGSERF 354
           +++   G++ K+      + +  +S RF +R     Y + +RF S RF
Sbjct: 308 EDNY--GQIEKSIVGFGKRDKGYESARFTDR-LIIGYSNPVRFSSGRF 349

[Close]

BLAST of Csa4G099230 vs. TAIR10
Match: AT5G65370.1 (AT5G65370.1 ENTH/ANTH/VHS superfamily protein)

HSP 1 Score: 146.4 bits (368), Expect = 3.5e-35
Identity = 94/274 (34.31%), Postives = 154/274 (56.20%), Query Frame = 1


Query: 14  KLSSLIGLIKDKASQSK---AALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSLG 73
           KL++L G++KD+ASQ K     L +  N  +  LALL+AT+H  + PPSDK+++ L S  
Sbjct: 3   KLATLNGILKDEASQMKLNVVHLCSSVNAKTIDLALLKATSHTSNNPPSDKYVTFLQSTI 62

Query: 74  KTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFK-----DGDFILQDQLS--VFP 133
            T        V+ ++ RL+ T +  VA KCLI +H + K     +G+  L++ ++     
Sbjct: 63  DT--CYGPDTVDAILHRLRVTTDVCVAAKCLILLHKMVKSESGYNGEDSLRNNINHRTLI 122

Query: 134 FTGGRNYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKER 193
           +T G + LKL+D   +S+  + +L+ WV+WY QY++  LSI+ +LG        NE+K  
Sbjct: 123 YTQGGSNLKLNDLNVNSSRFTRELTPWVQWYKQYLDCYLSIAEVLGITPNIKEKNEDKRL 182

Query: 194 KTEQISGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEI 253
           +T+++S      +LK+ + LV L E IS  P       N++V ++   +  DY SA++ +
Sbjct: 183 ETQRVSSYPMDCILKQIDFLVELFEHISDRPKAPQSKLNKIVIEMTELMVQDYFSAIRLM 242

Query: 254 SIRVTEFHHRLGWLSFAESVELVCALKRLEDCKE 278
            IR  E + R+     A+  ELV  L++LE+CKE
Sbjct: 243 RIRFEELNVRV-----AKPNELVPVLEKLENCKE 269

[Close]

BLAST of Csa4G099230 vs. TAIR10
Match: AT5G10410.1 (AT5G10410.1 ENTH/ANTH/VHS superfamily protein)

HSP 1 Score: 133.3 bits (334), Expect = 3.1e-31
Identity = 93/303 (30.69%), Postives = 153/303 (50.50%), Query Frame = 1


Query: 18  LIGLIKDKASQSKAALL---AKPNILSFQLALLRATTHDLHAPPSDKHLSALLSLGKTSR 77
           +IG  KDKAS  KA L+       +    LALL++TT   + PP+  ++SA++S   +  
Sbjct: 8   IIGKFKDKASIGKARLVHSFGSTAVKYIHLALLKSTTRTPNKPPNSDYVSAVISYSNSRY 67

Query: 78  ATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLKLS 137
           A AA      + RL+ T N+ VA K LI +H + K      +D+        GRN LKL+
Sbjct: 68  APAA--FSAALWRLRVTKNAIVATKSLIVIHKLIKSS----RDKFEGLGH--GRNNLKLN 127

Query: 138 DFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQISGILNS 197
           +F D S+ ++ +LS W+RWY QY++ +  + ++LG F     + ++K  + +++S     
Sbjct: 128 EFSDKSSNLTLELSQWIRWYGQYLDRLSWVPKVLGSFPNLLVNPKDKVEEKDRVSSYQTG 187

Query: 198 DLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTEFHHRL 257
            ++++T+SLV   E I   P    + +N++VD+I   V +DY   ++ + +R+     RL
Sbjct: 188 YIIRQTDSLVSFFEHICTRPEIPPMFQNKIVDEIRELVIEDYFKIVRLVMVRLQVLFERL 247

Query: 258 --------GWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQ---E 307
                   G L   +   L   L RL +CKE  S G+F +   L D  W  +  ++   E
Sbjct: 248 IKPGVKPIGDLGLNDFSLL---LVRLVECKESLS-GLFWRCRRLADDFWCLVEMLKAETE 298

[Close]

BLAST of Csa4G099230 vs. TAIR10
Match: AT2G01600.1 (AT2G01600.1 ENTH/ANTH/VHS superfamily protein)

HSP 1 Score: 95.9 bits (237), Expect = 5.4e-20
Identity = 61/193 (31.61%), Postives = 99/193 (51.30%), Query Frame = 1


Query: 20  GLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSLGKTSRATA-- 79
           G +KD  S     +          +A+++AT H +  PP D+HL  + +    +RA A  
Sbjct: 12  GALKD--STKVGLVRVNSEYADLDVAIVKATNH-VECPPKDRHLRKIFAATSVTRARADV 71

Query: 80  APAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLKLSDFR 139
           A  +  L  RL  T N  VALK LI +H + ++GD   +++L  F   G    L+LS+F+
Sbjct: 72  AYCIHALSRRLHKTRNWTVALKTLIVIHRLLREGDPTFREELLNFSQRG--RILQLSNFK 131

Query: 140 DSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSR---SNEEKERKTEQISGILNS 199
           D S+PI+WD S+WVR YA ++E  L   R+L +   + R   SN  +++   +   +   
Sbjct: 132 DDSSPIAWDCSAWVRTYALFLEERLECFRVLKYDTEAERLPKSNPGQDKGYSRTRDLDGE 191

Query: 200 DLLKETESLVGLI 208
           +LL++  +L  L+
Sbjct: 192 ELLEQLPALQQLL 199

[Close]

BLAST of Csa4G099230 vs. TAIR10
Match: AT5G57200.1 (AT5G57200.1 ENTH/ANTH/VHS superfamily protein)

HSP 1 Score: 93.2 bits (230), Expect = 3.5e-19
Identity = 60/197 (30.46%), Postives = 102/197 (51.78%), Query Frame = 1


Query: 20  GLIKDKASQSKAALLAKPN--ILSFQLALLRATTHDLHAPPSDKHLSALLSLGKT--SRA 79
           G +KD  +      LAK N       +A+++AT H + +PP ++H+  + S       RA
Sbjct: 12  GALKDTTTVG----LAKVNSEFKDLDIAIVKATNH-VESPPKERHVRKIFSATSVIQPRA 71

Query: 80  TAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLKLSD 139
             A  +  L  RL  T N  VA+K LI +H   ++GD   +++L    ++  R+ L++S+
Sbjct: 72  DVAYCIHALSKRLSKTRNWVVAMKVLIVIHRTLREGDPTFREEL--LNYSHRRHILRISN 131

Query: 140 FRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSR-----SNEEKERKTEQISG 199
           F+D ++P++WD S+WVR YA ++E  L   R+L + + + R         K  +T  +SG
Sbjct: 132 FKDDTSPLAWDCSAWVRTYALFLEERLECYRVLKYDIEAERLPKASGAASKTHRTRMLSG 191

Query: 200 ILNSDLLKETESLVGLI 208
               DLL++  +L  L+
Sbjct: 192 ---EDLLEQLPALQQLL 198

[Close]

BLAST of Csa4G099230 vs. NCBI nr
Match: gi|449439019|ref|XP_004137285.1| (PREDICTED: putative clathrin assembly protein At4g40080 [Cucumis sativus])

HSP 1 Score: 710.7 bits (1833), Expect = 1.3e-201
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 1


Query: 1   MLISLSLSMVNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSD 60
           MLISLSLSMVNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSD
Sbjct: 1   MLISLSLSMVNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSD 60

Query: 61  KHLSALLSLGKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLS 120
           KHLSALLSLGKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLS
Sbjct: 61  KHLSALLSLGKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLS 120

Query: 121 VFPFTGGRNYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEE 180
           VFPFTGGRNYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEE
Sbjct: 121 VFPFTGGRNYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEE 180

Query: 181 KERKTEQISGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAM 240
           KERKTEQISGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAM
Sbjct: 181 KERKTEQISGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAM 240

Query: 241 KEISIRVTEFHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRS 300
           KEISIRVTEFHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRS
Sbjct: 241 KEISIRVTEFHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRS 300

Query: 301 IQETKNLTGESKEHREGGKLCKTKRRVSDSGRFMERPNASSYRDLLRFGSERFVLTYDGF 360
           IQETKNLTGESKEHREGGKLCKTKRRVSDSGRFMERPNASSYRDLLRFGSERFVLTYDGF
Sbjct: 301 IQETKNLTGESKEHREGGKLCKTKRRVSDSGRFMERPNASSYRDLLRFGSERFVLTYDGF 360

Query: 361 Q 362
           Q
Sbjct: 361 Q 361

[Close]

BLAST of Csa4G099230 vs. NCBI nr
Match: gi|659111211|ref|XP_008455635.1| (PREDICTED: putative clathrin assembly protein At4g40080 [Cucumis melo])

HSP 1 Score: 594.0 bits (1530), Expect = 1.8e-166
Identity = 307/353 (86.97%), Postives = 325/353 (92.07%), Query Frame = 1


Query: 9   MVNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLS 68
           M+NTK+LSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHD HAPPSDKHLSALLS
Sbjct: 1   MMNTKRLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDPHAPPSDKHLSALLS 60

Query: 69  LGKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGR 128
           LGKTSRATAA AVEVLMDRLQTTHNSAVALKCLIAVHHIFK+G FILQDQLSVFPFTGGR
Sbjct: 61  LGKTSRATAAAAVEVLMDRLQTTHNSAVALKCLIAVHHIFKNGGFILQDQLSVFPFTGGR 120

Query: 129 NYLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQI 188
           NYLKLSDFRDSSNPISW+LSSWVRWYAQYIETVLSISRILGF VGSS SNEE ERKTEQI
Sbjct: 121 NYLKLSDFRDSSNPISWELSSWVRWYAQYIETVLSISRILGFIVGSSSSNEEMERKTEQI 180

Query: 189 SGILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVT 248
           SGI NS+LLK+TESLVGLIEEISKMP CLHLNRNRLVDKIY FVGDDYL+AMKEISIRVT
Sbjct: 181 SGIWNSELLKDTESLVGLIEEISKMPPCLHLNRNRLVDKIYGFVGDDYLAAMKEISIRVT 240

Query: 249 EFHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQETKNLT 308
           EFHHRLG LSF ESVELVCALKRL+D KEKQS+GIFA+YEVL+DG W SIR   ETKNL 
Sbjct: 241 EFHHRLGCLSFGESVELVCALKRLDDFKEKQSLGIFARYEVLMDGFWSSIR---ETKNLI 300

Query: 309 GESKEHREGGKLCKTKRRVSDSGRFMERPNASSYRDLLRFGSERFVLTYDGFQ 362
           G SKE+R+G KL + +RR+SDSGRF+ER NASSY D+L F SERF LTY GFQ
Sbjct: 301 GASKENRDGCKLSQMERRISDSGRFIERSNASSYCDVLPFRSERFGLTYKGFQ 350

[Close]

BLAST of Csa4G099230 vs. NCBI nr
Match: gi|590706824|ref|XP_007047831.1| (ENTH/ANTH/VHS superfamily protein, putative [Theobroma cacao])

HSP 1 Score: 375.9 bits (964), Expect = 7.7e-101
Identity = 196/342 (57.31%), Postives = 256/342 (74.85%), Query Frame = 1


Query: 15  LSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSLGKTSR 74
           L  LIG+IKDKASQSKAALL+ P  LS  LALLRATTHD   PP  +HL+ALLS G +SR
Sbjct: 7   LRDLIGIIKDKASQSKAALLSNPKTLSLHLALLRATTHDPFTPPDPRHLAALLSFGHSSR 66

Query: 75  ATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLKLS 134
           A AA A+E LMDRLQTT +++VA+KCL  +HHI K G FILQDQLSVFP TGGRNYLKLS
Sbjct: 67  AIAATAIEALMDRLQTTRDASVAIKCLFTIHHIIKRGSFILQDQLSVFPATGGRNYLKLS 126

Query: 135 DFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQISGILNS 194
           +FRD++ P++W+LSSWVRWYA Y+E++LS SRILGFF+ S+ S+ + +++ E++S ++NS
Sbjct: 127 NFRDNTTPLTWELSSWVRWYALYLESLLSTSRILGFFLCSTSSSVDIDKEEEKVSSLINS 186

Query: 195 DLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTEFHHRL 254
           +LL+E  SLV L+E+ISK P+ LH N N LV++I   VG+DYLS++ E+SIRV E   RL
Sbjct: 187 ELLREINSLVNLLEQISKSPNSLHANGNILVEEIQGLVGEDYLSSINEVSIRVGEVRERL 246

Query: 255 GWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQETKNLTGESKEH 314
             LSF +SVE VCALKRLEDCKE+ S+ +  + +VLID +WG   SI E K+  G SK +
Sbjct: 247 SSLSFVDSVEWVCALKRLEDCKER-SLALSQRKKVLIDAVWG---SISEIKDQVGSSKVY 306

Query: 315 REGGKLCK--TKRRVSDSGRFMERPNASSYRDLLRFGSERFV 355
           RE G+L    ++ + S+S RF ER     + D ++F S RF+
Sbjct: 307 REEGRLLTMGSRNKASESARFGER--VLKHGDSVKFSSGRFL 342

[Close]

BLAST of Csa4G099230 vs. NCBI nr
Match: gi|567867371|ref|XP_006426308.1| (hypothetical protein CICLE_v10025941mg [Citrus clementina])

HSP 1 Score: 368.6 bits (945), Expect = 1.2e-98
Identity = 189/346 (54.62%), Postives = 256/346 (73.99%), Query Frame = 1


Query: 10  VNTKKLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSL 69
           +N  +L++L+G+IKDK SQSKAA+++KP  L+  L+LLRATTHD   PP  K L+ LLS 
Sbjct: 1   MNMGRLANLMGIIKDKVSQSKAAIISKPKTLTLHLSLLRATTHDPSTPPDPKRLTTLLSF 60

Query: 70  GKTSRATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRN 129
           G +SRATAA  +E LMDRLQTTH+++VA+K LIAVHHI K G FILQDQLSV+P  GGRN
Sbjct: 61  GHSSRATAAAVIEALMDRLQTTHDASVAIKSLIAVHHIVKHGSFILQDQLSVYPSAGGRN 120

Query: 130 YLKLSDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQIS 189
           YLKLS+FRD++ P++W+LSSWVRWYA Y+E +LS SR+LGFF+ SS S+ E +++ E++S
Sbjct: 121 YLKLSNFRDNTTPLTWELSSWVRWYALYLEHLLSTSRVLGFFLSSSSSSVEMDKEEEKVS 180

Query: 190 GILNSDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTE 249
            ++N DLLKE +SL+ L+E++ K P CLH+  N LVD I   VG+DYLSA+ E+SIRV+E
Sbjct: 181 ALVNIDLLKEVDSLLSLLEQMCKTPDCLHVRGNPLVDDIMGLVGEDYLSAINEVSIRVSE 240

Query: 250 FHHRLGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQETKNLTG 309
           F++RLG LS  +SVEL CALKRLEDCKE+ S+ +  +  VLI+  WG I ++   K+   
Sbjct: 241 FNNRLGCLSLGDSVELACALKRLEDCKERLSV-LSHRKRVLIEAFWGLITAL---KDKVA 300

Query: 310 ESKEHREGGKLCKTKRR--VSDSGRFMERPNASSYRDLLRFGSERF 354
           + + +R+   +  T RR   S+S RF +R  +  Y D +RF S RF
Sbjct: 301 KERAYRDERMIVSTGRRDKASESARFGDR-LSRRYGDSVRFSSARF 341

[Close]

BLAST of Csa4G099230 vs. NCBI nr
Match: gi|568823763|ref|XP_006466278.1| (PREDICTED: putative clathrin assembly protein At4g40080 [Citrus sinensis])

HSP 1 Score: 365.5 bits (937), Expect = 1.0e-97
Identity = 188/342 (54.97%), Postives = 253/342 (73.98%), Query Frame = 1


Query: 14  KLSSLIGLIKDKASQSKAALLAKPNILSFQLALLRATTHDLHAPPSDKHLSALLSLGKTS 73
           +L++L+G+IKDK SQSKAA+++KP  L+  L+LLRATTHD   PP  K L+ LLS G +S
Sbjct: 3   RLANLMGIIKDKVSQSKAAIISKPKTLTLHLSLLRATTHDPSTPPDPKRLTTLLSFGHSS 62

Query: 74  RATAAPAVEVLMDRLQTTHNSAVALKCLIAVHHIFKDGDFILQDQLSVFPFTGGRNYLKL 133
           RATA+  +E LMDRLQTTH+++VA+K LIAVHHI K G FILQDQLSV+P  GGRNYLKL
Sbjct: 63  RATASAVIEALMDRLQTTHDASVAIKSLIAVHHIVKHGSFILQDQLSVYPSAGGRNYLKL 122

Query: 134 SDFRDSSNPISWDLSSWVRWYAQYIETVLSISRILGFFVGSSRSNEEKERKTEQISGILN 193
           S+FRD++ P++W+LSSWVRWYA Y+E +LS SR+LGFF+ SS S+ E +++ E++S ++N
Sbjct: 123 SNFRDNTTPLTWELSSWVRWYALYLEHLLSTSRVLGFFLSSSSSSVEMDKEEEKVSALVN 182

Query: 194 SDLLKETESLVGLIEEISKMPHCLHLNRNRLVDKIYSFVGDDYLSAMKEISIRVTEFHHR 253
            DLLKE +SL+ L+E++ K P CLH+  N LVD I   VG+DYLSA+ E+SIRV+EF++R
Sbjct: 183 IDLLKEVDSLLSLLEQMCKTPDCLHVRGNPLVDDIMGLVGEDYLSAINEVSIRVSEFNNR 242

Query: 254 LGWLSFAESVELVCALKRLEDCKEKQSMGIFAKYEVLIDGLWGSIRSIQETKNLTGESKE 313
           LG LS  +SVEL CALKRLEDCKE+ S+ +  +  VLI+  WG    I E K+   + + 
Sbjct: 243 LGCLSLGDSVELACALKRLEDCKERLSV-LSHRKRVLIEAFWG---LITELKDKVAKERA 302

Query: 314 HREGGKLCKTKRR--VSDSGRFMERPNASSYRDLLRFGSERF 354
           +R+   +  T RR   S+S RF +R  +  Y D +RF S RF
Sbjct: 303 YRDERMIVGTGRRDKASESARFGDR-LSRRYGDSVRFSSARF 339

The following BLAST results are available for this feature:

BLAST of Csa4G099230 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CAP16_ARATH	2.1e-82	49.71	Putative clathrin assembly protein At4g40080 OS=Arabidopsis thaliana GN=At4g4008...	[more]
CAP18_ARATH	6.2e-34	34.31	Putative clathrin assembly protein At5g65370 OS=Arabidopsis thaliana GN=At5g6537...	[more]
CAP17_ARATH	5.5e-30	30.69	Putative clathrin assembly protein At5g10410 OS=Arabidopsis thaliana GN=At5g1041...	[more]
CAP8_ARATH	9.6e-19	31.61	Putative clathrin assembly protein At2g01600 OS=Arabidopsis thaliana GN=At2g0160...	[more]
CAP7_ARATH	6.3e-18	30.46	Putative clathrin assembly protein At5g57200 OS=Arabidopsis thaliana GN=At5g5720...	[more]

BLAST of Csa4G099230 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXU4_CUCSA	9.2e-202	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G099230 PE=4 SV=1	[more]
A0A061DPV2_THECC	5.4e-101	57.31	ENTH/ANTH/VHS superfamily protein, putative OS=Theobroma cacao GN=TCM_001021 PE=...	[more]
V4RXC7_9ROSI	8.6e-99	54.62	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10025941mg PE=4 SV=1	[more]
A0A067EZX7_CITSI	8.6e-99	54.62	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g018185mg PE=4 SV=1	[more]
A0A0B0NU88_GOSAR	2.3e-96	55.01	Uncharacterized protein OS=Gossypium arboreum GN=F383_23111 PE=4 SV=1	[more]

BLAST of Csa4G099230 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G40080.1	1.2e-83	49.71	ENTH/ANTH/VHS superfamily protein	[more]
AT5G65370.1	3.5e-35	34.31	ENTH/ANTH/VHS superfamily protein	[more]
AT5G10410.1	3.1e-31	30.69	ENTH/ANTH/VHS superfamily protein	[more]
AT2G01600.1	5.4e-20	31.61	ENTH/ANTH/VHS superfamily protein	[more]
AT5G57200.1	3.5e-19	30.46	ENTH/ANTH/VHS superfamily protein	[more]

BLAST of Csa4G099230 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449439019\|ref\|XP_004137285.1\|	1.3e-201	100.00	PREDICTED: putative clathrin assembly protein At4g40080 [Cucumis sativus]	[more]
gi\|659111211\|ref\|XP_008455635.1\|	1.8e-166	86.97	PREDICTED: putative clathrin assembly protein At4g40080 [Cucumis melo]	[more]
gi\|590706824\|ref\|XP_007047831.1\|	7.7e-101	57.31	ENTH/ANTH/VHS superfamily protein, putative [Theobroma cacao]	[more]
gi\|567867371\|ref\|XP_006426308.1\|	1.2e-98	54.62	hypothetical protein CICLE_v10025941mg [Citrus clementina]	[more]
gi\|568823763\|ref\|XP_006466278.1\|	1.0e-97	54.97	PREDICTED: putative clathrin assembly protein At4g40080 [Citrus sinensis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008942	ENTH_VHS
IPR011417	ANTH_dom
IPR013809	ENTH

Vocabulary: **Molecular Function**
Term	Definition
GO:0005543	phospholipid binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044699	single-organism process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0005543	phospholipid binding

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU108630	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa4G099230.1	Csa4G099230.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU108630	CU108630	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008942	ENTH/VHS	GENE3D	G3DSA:1.25.40.90		coord: 43..166 score: 5.6
IPR008942	ENTH/VHS	unknown	SSF48464	ENTH/VHS domain	coord: 42..170 score: 1.23
IPR011417	AP180 N-terminal homology (ANTH) domain	PFAM	PF07651	ANTH	coord: 44..212 score: 1.5
IPR013809	ENTH domain	SMART	SM00273	enth_2	coord: 40..172 score: 6.8
IPR013809	ENTH domain	PROFILE	PS50942	ENTH	coord: 34..172 score: 17
None	No IPR available	PANTHER	PTHR22951	CLATHRIN ASSEMBLY PROTEIN	coord: 12..307 score: 7.5E
None	No IPR available	PANTHER	PTHR22951:SF18	SUBFAMILY NOT NAMED	coord: 12..307 score: 7.5E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa4G099230	MELO3C019004	Melon (DHL92) v3.5.1	cumeB303
Csa4G099230	ClCG10G008340	Watermelon (Charleston Gray)	cuwcgB276
Csa4G099230	MELO3C019004.2	Melon (DHL92) v3.6.1	cumedB288
Csa4G099230	CSPI04G08880	Wild cucumber (PI 183967)	cpicuB182
Csa4G099230	Cucsa.397730	Cucumber (Gy14) v1	cgycuB556
Csa4G099230	CmaCh16G007290	Cucurbita maxima (Rimu)	cmacuB335
Csa4G099230	Cp4.1LG14g00870	Cucurbita pepo (Zucchini)	cpecuB220
Csa4G099230	CsGy4G008700	Cucumber (Gy14) v2	cgybcuB165
Csa4G099230	Carg05844	Silver-seed gourd	carcuB1032

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa4G099230	Melon (DHL92) v3.6.1	cumedB308
Csa4G099230	Cucurbita moschata (Rifu)	cmocuB323
Csa4G099230	Melon (DHL92) v3.5.1	cumeB322