Csa4G099220 (gene) Cucumber (Chinese Long) v2

NameCsa4G099220
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
Description1-aminocyclopropane-1-carboxylate synthase; contains IPR015424 (Pyridoxal phosphate-dependent transferase)
LocationChr4 : 6483187 .. 6484884 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTTATTGTCTAGAAAAGCCACGTGTAACTTGCACGGCCAAGATTCGTCTTACTTCTCAGGATGGGAAGAATATGAGAAGAATCCTTACGATGAGATTCAAAATCCTAAAGGGATTATTCAAATGGGTCTGGCAGAAAATCAGGTCAGTAATAATAATCTATATGTGTGAGAGTATTAGAAATCAATAATTTGTTTTATTGTAAATATAAATATTTATCTTTTCATTATGGGTAACGAGAGAATGAAACTTTGATTTGCAATGGTGACATGCAGTTATGTTTTGATGTGGTGGAGAAGTGGCTGGAGGAGAACCCAAATTCGGCTGGTTTTAAGAGAGATGGAAAGTCCATTTTTAGACAACTTGCTCTTTTTCAAGACTACAATGGCTTGCCTGCATTCAAAAAGGTACAGAATAATACATTACTTATATTCTTCTTTAATTGTTTTTGTATAATATTATGTAAAGTTAAGTTTATGACATCAGTCTTTGGGAGATCACCATGCAATTTAACGATAATGAATAATAATGTTTCAGGCATTAGCAGAGTTCATGTCGGAAATTAGAGGGAACAAAGTAGCGTTTGATCCCAACCACATAGTCCTGACTGCTGGTGCCACCTCAGCTAACGAGACTCTTATGTTCTGCTTGGCTGAACCTGGCGACGCCTTTCTTCTACCAACTCCCTACTATCCAGGGTACGTTTCAATTTCATAATTTCTATTAAAGAAAAATAAACAAATATTATCTGCATTTGGCAACTGTCCCTCACCTTGGTCTTTGCTTTTATTCAATGTAATATACAGATTCGATAGAGACTTGAAATGGAGAACTGGAATTGAGATCGTACCCATCCACTGCTCCAGCTCCAATGGCTTCAAAATAACAGAATCAGCATTGTTAGAAGCCTACCAAAACGCCTTAAATTGTAACCTCAAAGTCAAAGGCGTACTTGTTACGAACCCATCGAACCCATTAGGAACCACAATCAGTAGTCACGAACTCAACCTCCTACTTCAATTCATCGAATCAAAAAACCTCCATCTAATCAGCGACGAGATTTACTCTGGCACAGCTTTCCGAAACTCGGGTTTTGTCAGTGCCATGGAAATCCTTAACCAAACATACAAACACAACCACCAAATCCAAAATCAAGTTCACATTGTTTACAGCCTCTCCAAAGATCTTGGCCTGCCCGGGTTCCGAGTCGGAGCCTTATATTCGAATGACAAAGTAGTAGTGGCTGCAGCGACAAAGATGTCGAGCTTTGGATTGGTTTCGTCACAAACGCAGTACCTTCTGTCGGCGATGTTGTCAGACAAGAAGTTTAGGAGGAAGTACATTAGAAAGAACCAAAAGAGGTTGGAGAAAAGACAGACAATGCTGGTGAATGGGCTGGAGAAGGCGGGGATTAAGTGCCACAAAGGGAATGCAGGGCTTTTTTGCTGGGTGGATATGAGGCATTTGTTGGGAGGCAATACGTTTGAGTCAGAAATGGAGCTTTGGAGGAAGATTCTATTCGAGGTGCGCTTGAACGTGTCACCCGGACAGTCATGCCATTGCTCCGAACCAGGTTGGTTTCGCATTTGTTTTGCCAACATGTCTGAACCAACTTTGGATCTTGCAATGCAGAGATTGAAGGCTTTTGTTGTCAACTACTCCACCACAATTACGGTAACTTCTCCGCAGATGTGA

mRNA sequence

ATGGGGTTATTGTCTAGAAAAGCCACGTGTAACTTGCACGGCCAAGATTCGTCTTACTTCTCAGGATGGGAAGAATATGAGAAGAATCCTTACGATGAGATTCAAAATCCTAAAGGGATTATTCAAATGGGTCTGGCAGAAAATCAGTTATGTTTTGATGTGGTGGAGAAGTGGCTGGAGGAGAACCCAAATTCGGCTGGTTTTAAGAGAGATGGAAAGTCCATTTTTAGACAACTTGCTCTTTTTCAAGACTACAATGGCTTGCCTGCATTCAAAAAGGCATTAGCAGAGTTCATGTCGGAAATTAGAGGGAACAAAGTAGCGTTTGATCCCAACCACATAGTCCTGACTGCTGGTGCCACCTCAGCTAACGAGACTCTTATGTTCTGCTTGGCTGAACCTGGCGACGCCTTTCTTCTACCAACTCCCTACTATCCAGGATTCGATAGAGACTTGAAATGGAGAACTGGAATTGAGATCGTACCCATCCACTGCTCCAGCTCCAATGGCTTCAAAATAACAGAATCAGCATTGTTAGAAGCCTACCAAAACGCCTTAAATTGTAACCTCAAAGTCAAAGGCGTACTTGTTACGAACCCATCGAACCCATTAGGAACCACAATCAGTAGTCACGAACTCAACCTCCTACTTCAATTCATCGAATCAAAAAACCTCCATCTAATCAGCGACGAGATTTACTCTGGCACAGCTTTCCGAAACTCGGGTTTTGTCAGTGCCATGGAAATCCTTAACCAAACATACAAACACAACCACCAAATCCAAAATCAAGTTCACATTGTTTACAGCCTCTCCAAAGATCTTGGCCTGCCCGGGTTCCGAGTCGGAGCCTTATATTCGAATGACAAAGTAGTAGTGGCTGCAGCGACAAAGATGTCGAGCTTTGGATTGGTTTCGTCACAAACGCAGTACCTTCTGTCGGCGATGTTGTCAGACAAGAAGTTTAGGAGGAAGTACATTAGAAAGAACCAAAAGAGGTTGGAGAAAAGACAGACAATGCTGGTGAATGGGCTGGAGAAGGCGGGGATTAAGTGCCACAAAGGGAATGCAGGGCTTTTTTGCTGGGTGGATATGAGGCATTTGTTGGGAGGCAATACGTTTGAGTCAGAAATGGAGCTTTGGAGGAAGATTCTATTCGAGGTGCGCTTGAACGTGTCACCCGGACAGTCATGCCATTGCTCCGAACCAGGTTGGTTTCGCATTTGTTTTGCCAACATGTCTGAACCAACTTTGGATCTTGCAATGCAGAGATTGAAGGCTTTTGTTGTCAACTACTCCACCACAATTACGGTAACTTCTCCGCAGATGTGA

Coding sequence (CDS)

ATGGGGTTATTGTCTAGAAAAGCCACGTGTAACTTGCACGGCCAAGATTCGTCTTACTTCTCAGGATGGGAAGAATATGAGAAGAATCCTTACGATGAGATTCAAAATCCTAAAGGGATTATTCAAATGGGTCTGGCAGAAAATCAGTTATGTTTTGATGTGGTGGAGAAGTGGCTGGAGGAGAACCCAAATTCGGCTGGTTTTAAGAGAGATGGAAAGTCCATTTTTAGACAACTTGCTCTTTTTCAAGACTACAATGGCTTGCCTGCATTCAAAAAGGCATTAGCAGAGTTCATGTCGGAAATTAGAGGGAACAAAGTAGCGTTTGATCCCAACCACATAGTCCTGACTGCTGGTGCCACCTCAGCTAACGAGACTCTTATGTTCTGCTTGGCTGAACCTGGCGACGCCTTTCTTCTACCAACTCCCTACTATCCAGGATTCGATAGAGACTTGAAATGGAGAACTGGAATTGAGATCGTACCCATCCACTGCTCCAGCTCCAATGGCTTCAAAATAACAGAATCAGCATTGTTAGAAGCCTACCAAAACGCCTTAAATTGTAACCTCAAAGTCAAAGGCGTACTTGTTACGAACCCATCGAACCCATTAGGAACCACAATCAGTAGTCACGAACTCAACCTCCTACTTCAATTCATCGAATCAAAAAACCTCCATCTAATCAGCGACGAGATTTACTCTGGCACAGCTTTCCGAAACTCGGGTTTTGTCAGTGCCATGGAAATCCTTAACCAAACATACAAACACAACCACCAAATCCAAAATCAAGTTCACATTGTTTACAGCCTCTCCAAAGATCTTGGCCTGCCCGGGTTCCGAGTCGGAGCCTTATATTCGAATGACAAAGTAGTAGTGGCTGCAGCGACAAAGATGTCGAGCTTTGGATTGGTTTCGTCACAAACGCAGTACCTTCTGTCGGCGATGTTGTCAGACAAGAAGTTTAGGAGGAAGTACATTAGAAAGAACCAAAAGAGGTTGGAGAAAAGACAGACAATGCTGGTGAATGGGCTGGAGAAGGCGGGGATTAAGTGCCACAAAGGGAATGCAGGGCTTTTTTGCTGGGTGGATATGAGGCATTTGTTGGGAGGCAATACGTTTGAGTCAGAAATGGAGCTTTGGAGGAAGATTCTATTCGAGGTGCGCTTGAACGTGTCACCCGGACAGTCATGCCATTGCTCCGAACCAGGTTGGTTTCGCATTTGTTTTGCCAACATGTCTGAACCAACTTTGGATCTTGCAATGCAGAGATTGAAGGCTTTTGTTGTCAACTACTCCACCACAATTACGGTAACTTCTCCGCAGATGTGA

Protein sequence

MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLEENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGATSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLEAYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRNSGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSSFGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFCWVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLAMQRLKAFVVNYSTTITVTSPQM*
BLAST of Csa4G099220 vs. Swiss-Prot
Match: 1A13_SOLLC (1-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum GN=ACS3 PE=1 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 2.5e-194
Identity = 325/436 (74.54%), Postives = 376/436 (86.24%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M LLS KATCN HGQDSSYF GW+EYEKNPYDEIQNPKGIIQMGLAENQL FD++E WL 
Sbjct: 1   MKLLSEKATCNSHGQDSSYFLGWQEYEKNPYDEIQNPKGIIQMGLAENQLSFDLLESWLA 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++AGFKR+G+SIFR+LALFQDY+GLPAFK A+ +FMSEIRGN+V+FD N++VLTAGA
Sbjct: 61  QNPDAAGFKRNGESIFRELALFQDYHGLPAFKNAMTKFMSEIRGNRVSFDSNNLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLA  GDAFLLPTPYYPGFDRDLKWRTG EIVPIHCSSSNGF+ITESAL E
Sbjct: 121 TSANETLMFCLANQGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFRITESALEE 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AY +A   NLKVKGVLVTNPSNPLGTT++ +EL LLL FI+ K +HLISDEIYSGT F +
Sbjct: 181 AYLDAKKRNLKVKGVLVTNPSNPLGTTLNRNELELLLTFIDEKGIHLISDEIYSGTVFNS 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
            GFVS ME+L +      ++  +VHIVYSLSKDLGLPGFR+GA+YSND++VV+AATKMSS
Sbjct: 241 PGFVSVMEVLIEKNYMKTRVWERVHIVYSLSKDLGLPGFRIGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLS MLSDKKF +KYI +NQKRL+KR  MLV GL+ AGI C + NAGLFC
Sbjct: 301 FGLVSSQTQYLLSCMLSDKKFTKKYISENQKRLKKRHAMLVKGLKSAGINCLESNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLL  N F++EM+LW+KI+++V LN+SPG SCHC+EPGWFR+CFANMSE TLDLA
Sbjct: 361 WVDMRHLLSSNNFDAEMDLWKKIVYDVGLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MQRLKAFVVNYSTTIT 437
           M+R+K FV + +   T
Sbjct: 421 MRRIKDFVESTAPNAT 436

BLAST of Csa4G099220 vs. Swiss-Prot
Match: 1A19_ARATH (1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana GN=ACS9 PE=1 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 2.1e-193
Identity = 324/428 (75.70%), Postives = 369/428 (86.21%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M  LSRK T N HGQDSSYF GWEEYEKNPYDEI+NP GIIQMGLAENQLCFD++E WL 
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++AG K+DG+SIF++LALFQDY+GLP FKKALAEFM EIRGN+V FDP+ IVL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPIHCSSSNGF+ITESAL +
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ A   +LKVKGVLVTNPSNPLGT ++  ELNLL+ FI SKN+HLISDEIYSGT F  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
             FVS M++L      N ++  +VHIVYSLSKDLGLPGFRVGA+YSND++VV+AATKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKF   Y+ +NQKRL+ RQ  LV+GLE AGI C K NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLL  NTFE+E+ELW+KI+++V+LN+SPG SCHC+EPGWFR+CFANMSE TLDLA
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MQRLKAFV 429
           M+RLK +V
Sbjct: 421 MKRLKEYV 428

BLAST of Csa4G099220 vs. Swiss-Prot
Match: 1A12_CUCMA (1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima GN=ACS2 PE=2 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 2.3e-192
Identity = 324/436 (74.31%), Postives = 373/436 (85.55%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M +LS KATCN HGQDSSYF GWE YE NP+    NP GIIQMGLAENQL FD++E WL 
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++A FKRDGKSIFR+LALFQDY+GLPAFKKAL EFM+EIRGNKV+F+ N+IVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG+EIVPIHC+SSNGF+IT+SAL +
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITQSALEQ 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AY++A   NL+VKGVLVTNPSNPLGTT++  ELNL+  FI SK +HLISDEIYSGT F +
Sbjct: 181 AYKDAQTRNLRVKGVLVTNPSNPLGTTMNRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
            GFVSAME+L +    + ++  +VHIVYSLSKDLGLPGFRVGA+YSND +VVAAATKMSS
Sbjct: 241 PGFVSAMEVLKERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKF   YI +NQKRL++RQ MLV+GL+KAGI C   NAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTISYISENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLL  + FESE+ELW+KI++EV LN+SPG SCHC+EPGWFR+CFANMSE TL LA
Sbjct: 361 WVDMRHLLESDKFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 MQRLKAFVVNYSTTIT 437
           ++RLK+FV    +T T
Sbjct: 421 VRRLKSFVTELRSTTT 436

BLAST of Csa4G099220 vs. Swiss-Prot
Match: 1A1C_MALDO (1-aminocyclopropane-1-carboxylate synthase OS=Malus domestica GN=ACS-1 PE=1 SV=2)

HSP 1 Score: 667.2 bits (1720), Expect = 1.3e-190
Identity = 316/432 (73.15%), Postives = 371/432 (85.88%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M +LSR AT N HGQDSSYF GW+EYEKNPY E+ N  GIIQMGLAENQLCFD++E WL 
Sbjct: 1   MRMLSRNATFNSHGQDSSYFLGWQEYEKNPYHEVHNTNGIIQMGLAENQLCFDLLESWLA 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP +A FK++G+SIF +LALFQDY+GLPAFKKA+ +FM+EIRGNKV FDPNH+VLTAGA
Sbjct: 61  KNPEAAAFKKNGESIFAELALFQDYHGLPAFKKAMVDFMAEIRGNKVTFDPNHLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANET +FCLA+PG+A L+PTPYYPGFDRDLKWRTG+EIVPIHC+SSNGF+ITE+AL E
Sbjct: 121 TSANETFIFCLADPGEAVLIPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITETALEE 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ A   NL+VKGVLVTNPSNPLGTT++ +EL LLL F+E K +HLISDEIYSGTAF +
Sbjct: 181 AYQEAEKRNLRVKGVLVTNPSNPLGTTMTRNELYLLLSFVEDKGIHLISDEIYSGTAFSS 240

Query: 241 SGFVSAMEIL-NQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMS 300
             F+S ME+L ++    N ++  +VH+VYSLSKDLGLPGFRVGA+YSND +VVAAATKMS
Sbjct: 241 PSFISVMEVLKDRNCDENSEVWQRVHVVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLF 360
           SFGLVSSQTQ+LLSAMLSDKK  + YI +N KRL++RQ  LV+GL+K+GI C  GNAGLF
Sbjct: 301 SFGLVSSQTQHLLSAMLSDKKLTKNYIAENHKRLKQRQKKLVSGLQKSGISCLNGNAGLF 360

Query: 361 CWVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDL 420
           CWVDMRHLL  NTFE+EMELW+KI++EV LN+SPG SCHC+EPGWFR+CFAN+ E TLDL
Sbjct: 361 CWVDMRHLLRSNTFEAEMELWKKIVYEVHLNISPGSSCHCTEPGWFRVCFANLPERTLDL 420

Query: 421 AMQRLKAFVVNY 432
           AMQRLKAFV  Y
Sbjct: 421 AMQRLKAFVGEY 432

BLAST of Csa4G099220 vs. Swiss-Prot
Match: 1A15_ARATH (1-aminocyclopropane-1-carboxylate synthase 5 OS=Arabidopsis thaliana GN=ACS5 PE=1 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 4.9e-190
Identity = 314/428 (73.36%), Postives = 366/428 (85.51%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M  LS K T N HGQDSSYF GWEEYEKNPYDEI+NP G+IQMGLAENQLCFD++E WL 
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++A  KR+G+SIFR+LALFQDY+G+P FKKA+AEFM EIRGN+V FDP  IVL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPIHCSSSNGF+ITESAL +
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ A   +LKVKGVLVTNPSNPLGT ++  ELNLL+ FI SKN+HLISDEIYSGT F  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
             F+S M++L      + ++  +VH+VYSLSKDLGLPGFRVGA+YSND+++V+AATKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKF  +Y+ +NQKRL+ RQ  LV+GLE AGI C + NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLL  NTFE+E++LW+KI++ V+LN+SPG SCHC+EPGWFR+CFANMSE TLDLA
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MQRLKAFV 429
           ++RLK FV
Sbjct: 421 LKRLKTFV 428

BLAST of Csa4G099220 vs. TrEMBL
Match: W9RA06_9ROSA (1-aminocyclopropane-1-carboxylate synthase OS=Morus notabilis GN=L484_007775 PE=3 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 2.8e-200
Identity = 335/433 (77.37%), Postives = 391/433 (90.30%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M LLSRKA+CN HGQDSSYF GW+EYEKNPYDE++NPKGIIQMGLAENQL F ++E WL 
Sbjct: 1   MRLLSRKASCNTHGQDSSYFLGWQEYEKNPYDEVKNPKGIIQMGLAENQLSFHLLESWLA 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++AGFKRDGKSIFR+LALFQDY+GLPAFK+AL +FMSEIRGNKV FDPNHIVLTAGA
Sbjct: 61  KNPDAAGFKRDGKSIFRELALFQDYHGLPAFKQALVDFMSEIRGNKVTFDPNHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPG+AFLLPTPYYPGFDRDLKWRTG+EIVPIHC+SSNGF+ITE+AL +
Sbjct: 121 TSANETLMFCLAEPGEAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITEAALNQ 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           A++ A N NL+VKGVLVTNPSNPLGTT++S+E NLL+ FIE+K +HLISDEIYSGT F +
Sbjct: 181 AFEEAENRNLRVKGVLVTNPSNPLGTTMTSNEHNLLVSFIEAKKIHLISDEIYSGTVFTS 240

Query: 241 SGFVSAMEIL-NQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMS 300
            GFVS MEIL N+ Y    ++  +VH+VYSLSKDLGLPGFRVGA+YSND++VVAAATKMS
Sbjct: 241 PGFVSVMEILKNRNYDKFSEVWKRVHVVYSLSKDLGLPGFRVGAIYSNDEMVVAAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLF 360
           SFGLVSSQTQYLLSAMLSD+ F +KYI +NQKRL+KR  MLV+GL+KAG+ C K NAGLF
Sbjct: 301 SFGLVSSQTQYLLSAMLSDRNFTKKYISENQKRLKKRHGMLVSGLQKAGVSCLKSNAGLF 360

Query: 361 CWVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDL 420
           CWVDMRHLL  NTFE+EMELW+KI+++V+LN+SPG SCHC+EPGWFR+CFANMSE TL+L
Sbjct: 361 CWVDMRHLLRSNTFEAEMELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEETLEL 420

Query: 421 AMQRLKAFVVNYS 433
           AMQRLK+FV  Y+
Sbjct: 421 AMQRLKSFVTEYN 433

BLAST of Csa4G099220 vs. TrEMBL
Match: V4SQK3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027248mg PE=3 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.3e-197
Identity = 337/435 (77.47%), Postives = 382/435 (87.82%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           MGLLS KATCN HGQDSSYF GW+EYEKNPYDE+QNP GIIQMGLAENQL FD++E WL+
Sbjct: 1   MGLLSGKATCNTHGQDSSYFLGWQEYEKNPYDEVQNPTGIIQMGLAENQLSFDLLESWLD 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
            NP++AGFKRDG+SIFR+LALFQDY+GLPAFKKAL EFMSEIRGNKV FD + IVLTAGA
Sbjct: 61  RNPDAAGFKRDGQSIFRELALFQDYHGLPAFKKALVEFMSEIRGNKVTFDTSKIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPG+AFLLPTPYYPGFDRDLKWRTG+EIVPI C+S NGF+ITE AL E
Sbjct: 121 TSANETLMFCLAEPGEAFLLPTPYYPGFDRDLKWRTGVEIVPIQCTSLNGFQITEMALEE 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ A   +LKVKGVLVTNPSNPLGTT+S +ELNLL+ FI  KN+HLISDEIYSGT F +
Sbjct: 181 AYQLAQKRHLKVKGVLVTNPSNPLGTTMSRNELNLLVNFITEKNIHLISDEIYSGTVFSS 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
            GFVS MEIL      N ++ N+VHIVYSLSKDLGLPGFRVGA+YSND  VVAAATKMSS
Sbjct: 241 PGFVSIMEILKDRNCENTEVWNRVHIVYSLSKDLGLPGFRVGAIYSNDDTVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKF + YI +N+KRL++R   LV+GLEKAGI C K NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFAQNYISENKKRLKQRHRKLVSGLEKAGISCLKSNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLL  NTFE+EMELW+KI+++V+LN+SPG SCHC+EPGWFR+CFANMSE TL L+
Sbjct: 361 WVDMRHLLSSNTFEAEMELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLKLS 420

Query: 421 MQRLKAFVVNYSTTI 436
           MQRLKAFV + +TT+
Sbjct: 421 MQRLKAFVDSITTTL 435

BLAST of Csa4G099220 vs. TrEMBL
Match: A0A067GGJ6_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g043923mg PE=3 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 3.8e-197
Identity = 336/435 (77.24%), Postives = 383/435 (88.05%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           MGLLS KATCN HGQDSSYF GW+EYEKNPYDE+QNP GIIQMGLAENQL FD++E WL+
Sbjct: 1   MGLLSGKATCNTHGQDSSYFLGWQEYEKNPYDEVQNPTGIIQMGLAENQLSFDLLESWLD 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
            NP++AGFKRDG+SIFR+LALFQDY+GLPAFKKAL EFMSEIRGNKV FD + IVLTAGA
Sbjct: 61  RNPDAAGFKRDGQSIFRELALFQDYHGLPAFKKALVEFMSEIRGNKVTFDTSKIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPG+AFLLPTPYYPGFDRDLKWRTG+EIVPI C+S NGF+ITE+A+ E
Sbjct: 121 TSANETLMFCLAEPGEAFLLPTPYYPGFDRDLKWRTGVEIVPIQCTSLNGFQITETAIEE 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ A   +LKVKGVLVTNPSNPLGTT+S +ELNLL+  I  KN+HLISDEIYSGT F +
Sbjct: 181 AYQLAQKRHLKVKGVLVTNPSNPLGTTMSRNELNLLVNIITEKNIHLISDEIYSGTVFSS 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
            GFVS MEIL      N Q+ N+VHIVYSLSKDLGLPGFRVGA+YSND +VVAAATKMSS
Sbjct: 241 PGFVSIMEILKDRNCENTQVWNRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKF + YI +N+KRL++R   LV+GLEKAGI C K NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTQNYISENKKRLKQRHRKLVSGLEKAGISCLKSNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLL  NTFE+EMELW+KI+++V+LN+SPG SCHC+EPGWFR+CFANMSE TL L+
Sbjct: 361 WVDMRHLLRSNTFEAEMELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLKLS 420

Query: 421 MQRLKAFVVNYSTTI 436
           MQRLKAFV + +TT+
Sbjct: 421 MQRLKAFVDSITTTL 435

BLAST of Csa4G099220 vs. TrEMBL
Match: Q8S935_DIOKA (1-aminocyclopropane-1-carboxylate synthase OS=Diospyros kaki GN=DK-ACS1 PE=2 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 3.9e-194
Identity = 331/429 (77.16%), Postives = 373/429 (86.95%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M LLSRKA CN HGQDSSYF GW+EYEKNPYDE  NP GIIQMGLAENQL FD++E WL 
Sbjct: 1   MKLLSRKAACNTHGQDSSYFLGWQEYEKNPYDESHNPTGIIQMGLAENQLSFDLLESWLA 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
            NP++AGFKRDG+SIFR+LALFQDY+GLP FKKAL EFMSEIRGNKV+FDP  +VLTAGA
Sbjct: 61  SNPDAAGFKRDGQSIFRELALFQDYHGLPDFKKALVEFMSEIRGNKVSFDPKKLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLA+PG+AFLLPTPYYPGFDRDLKWRTG+EIVPI C+SSNGF+ITESAL E
Sbjct: 121 TSANETLMFCLADPGEAFLLPTPYYPGFDRDLKWRTGVEIVPIKCTSSNGFRITESALEE 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ A   NLKVKGVLVTNPSNPLGTT+S HELNLLL F+  K +HLISDEIYSGT F +
Sbjct: 181 AYQAAGKRNLKVKGVLVTNPSNPLGTTLSRHELNLLLSFVTEKGIHLISDEIYSGTVFSS 240

Query: 241 SGFVSAMEIL-NQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMS 300
            GF+S MEIL ++ Y  N ++  +VHIVYSLSKDLGLPGFRVGA+YSND VVV AATKMS
Sbjct: 241 PGFLSVMEILMDKKYSMNTEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDVVVEAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLF 360
           SFGLVSSQTQYLLSAMLSDKKFR+ YI +NQKRL  RQ ML++GLE AGI+C K NAGLF
Sbjct: 301 SFGLVSSQTQYLLSAMLSDKKFRKNYISENQKRLRHRQEMLISGLESAGIRCLKSNAGLF 360

Query: 361 CWVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDL 420
           CWVDMRHLL   TF++EMELW+KI+++V LN+SPG SCHC EPGWFR+CFANMS  TL+L
Sbjct: 361 CWVDMRHLLSSETFDAEMELWKKIVYDVGLNISPGSSCHCDEPGWFRVCFANMSAATLNL 420

Query: 421 AMQRLKAFV 429
           A+QR+K FV
Sbjct: 421 AIQRIKLFV 429

BLAST of Csa4G099220 vs. TrEMBL
Match: B2XCJ7_9ROSA (1-aminocyclopropane 1-carboxylate synthase 1 OS=Prunus salicina GN=ACS1 PE=2 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 6.6e-194
Identity = 327/432 (75.69%), Postives = 376/432 (87.04%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M +LS KATCN HGQDSSYF GW+EYEKNPY E+QN KGIIQMGLAENQL FD++E WL 
Sbjct: 1   MCMLSTKATCNSHGQDSSYFLGWQEYEKNPYHEVQNTKGIIQMGLAENQLSFDILESWLA 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++AGFKR G+SIF +LALFQDY+GLPAFKKAL +FM+EIRGNKV FDPNH+VLTAGA
Sbjct: 61  KNPDAAGFKRSGESIFAELALFQDYHGLPAFKKALVDFMAEIRGNKVTFDPNHLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETL+FCLA PG+A L+PTPYYPGFDRDLKWRTG+EIVPIHC+SSNGF+ITE+AL E
Sbjct: 121 TSANETLIFCLANPGEAVLIPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITETALQE 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ A   NL+VKGVLVTNPSNPLGTT++  ELNLLL F+E K +HLISDEIYSGT F +
Sbjct: 181 AYQAAQKRNLRVKGVLVTNPSNPLGTTMTRSELNLLLSFVEDKGIHLISDEIYSGTVFSS 240

Query: 241 SGFVSAMEILNQ-TYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMS 300
             F S ME+L +     N  + N+VH+VYSLSKDLGLPGFRVGA+YSND +VVAAATKMS
Sbjct: 241 PSFTSVMEVLKERKCDENSPMWNRVHVVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLF 360
           SFGL+SSQTQYLLSAMLSDKK  + YI +NQKRL++RQ  LV+GL+KAGI C KGNAGLF
Sbjct: 301 SFGLISSQTQYLLSAMLSDKKLTKNYISENQKRLKQRQKRLVSGLQKAGISCLKGNAGLF 360

Query: 361 CWVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDL 420
           CWVDMRHLL  NTFE+EMELW+KI++EVRLN+SPG SCHC+EPGWFR+CFAN+SE TLDL
Sbjct: 361 CWVDMRHLLRSNTFEAEMELWKKIVYEVRLNISPGSSCHCTEPGWFRVCFANLSEKTLDL 420

Query: 421 AMQRLKAFVVNY 432
           AM RLKAFV  Y
Sbjct: 421 AMHRLKAFVGEY 432

BLAST of Csa4G099220 vs. TAIR10
Match: AT3G49700.1 (AT3G49700.1 1-aminocyclopropane-1-carboxylate synthase 9)

HSP 1 Score: 676.4 bits (1744), Expect = 1.2e-194
Identity = 324/428 (75.70%), Postives = 369/428 (86.21%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M  LSRK T N HGQDSSYF GWEEYEKNPYDEI+NP GIIQMGLAENQLCFD++E WL 
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++AG K+DG+SIF++LALFQDY+GLP FKKALAEFM EIRGN+V FDP+ IVL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPIHCSSSNGF+ITESAL +
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ A   +LKVKGVLVTNPSNPLGT ++  ELNLL+ FI SKN+HLISDEIYSGT F  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
             FVS M++L      N ++  +VHIVYSLSKDLGLPGFRVGA+YSND++VV+AATKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKF   Y+ +NQKRL+ RQ  LV+GLE AGI C K NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLL  NTFE+E+ELW+KI+++V+LN+SPG SCHC+EPGWFR+CFANMSE TLDLA
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MQRLKAFV 429
           M+RLK +V
Sbjct: 421 MKRLKEYV 428

BLAST of Csa4G099220 vs. TAIR10
Match: AT5G65800.1 (AT5G65800.1 ACC synthase 5)

HSP 1 Score: 665.2 bits (1715), Expect = 2.7e-191
Identity = 314/428 (73.36%), Postives = 366/428 (85.51%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M  LS K T N HGQDSSYF GWEEYEKNPYDEI+NP G+IQMGLAENQLCFD++E WL 
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++A  KR+G+SIFR+LALFQDY+G+P FKKA+AEFM EIRGN+V FDP  IVL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPIHCSSSNGF+ITESAL +
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ A   +LKVKGVLVTNPSNPLGT ++  ELNLL+ FI SKN+HLISDEIYSGT F  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
             F+S M++L      + ++  +VH+VYSLSKDLGLPGFRVGA+YSND+++V+AATKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKF  +Y+ +NQKRL+ RQ  LV+GLE AGI C + NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLL  NTFE+E++LW+KI++ V+LN+SPG SCHC+EPGWFR+CFANMSE TLDLA
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MQRLKAFV 429
           ++RLK FV
Sbjct: 421 LKRLKTFV 428

BLAST of Csa4G099220 vs. TAIR10
Match: AT4G37770.1 (AT4G37770.1 1-amino-cyclopropane-1-carboxylate synthase 8)

HSP 1 Score: 657.1 bits (1694), Expect = 7.5e-189
Identity = 312/434 (71.89%), Postives = 365/434 (84.10%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           MGLLS+KA+CN HGQDSSYF GWEEYEKNPYDEI+NP GIIQMGLAENQL FD++E WL 
Sbjct: 1   MGLLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLA 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++A F+R+G+SIFR+LALFQDY+GLP+FK A+A+FMSE RGN+V+F+PN +VLTAGA
Sbjct: 61  KNPDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           T ANETLMFCLA+PGDAFLLPTPYYPGFDRDLKWRTG EIVPI C S+NGF+IT+ AL E
Sbjct: 121 TPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEE 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AY+ A   NLKVKGVL+TNPSNPLGTT +  ELN LL FI  K +HLISDEIYSGT F N
Sbjct: 181 AYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTN 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
            GF+S ME+L      N  + ++VHIVYSLSKDLGLPGFRVG +YSND  VV+AATKMSS
Sbjct: 241 PGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGL+SSQTQYLLSA+LSDK F + Y+ +NQ RL+ R   LV+GLE AGI+C K NAGLFC
Sbjct: 301 FGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLL  NTFE+E+ELW+KI++EV+LN+SPG SCHC+EPGWFR+CFAN+SE TL +A
Sbjct: 361 WVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVA 420

Query: 421 MQRLKAFVVNYSTT 435
           + RLK FV   S T
Sbjct: 421 LDRLKRFVDGPSPT 434

BLAST of Csa4G099220 vs. TAIR10
Match: AT2G22810.1 (AT2G22810.1 1-aminocyclopropane-1-carboxylate synthase 4)

HSP 1 Score: 637.1 bits (1642), Expect = 8.0e-183
Identity = 305/426 (71.60%), Postives = 359/426 (84.27%), Query Frame = 1

Query: 4   LSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLEENP 63
           LSRKATCN HGQ SSYF GWEEYEKNPYD  +NP+GIIQMGLAENQLCFD++E WL +N 
Sbjct: 4   LSRKATCNSHGQVSSYFLGWEEYEKNPYDVTKNPQGIIQMGLAENQLCFDLLESWLAQNT 63

Query: 64  NSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGATSA 123
           ++A FKRDG+S+FR+LALFQDY+GL +FK A A+FMSE RGN+V+FD N++VLTAGATSA
Sbjct: 64  DAACFKRDGQSVFRELALFQDYHGLSSFKNAFADFMSENRGNRVSFDSNNLVLTAGATSA 123

Query: 124 NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLEAYQ 183
           NETLMFCLA+PGDAFLLPTPYYPGFDRDLKWRTG+EIVPI  SS+NGF+IT+ AL EAY+
Sbjct: 124 NETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGVEIVPIQSSSTNGFRITKLALEEAYE 183

Query: 184 NALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFI-ESKNLHLISDEIYSGTAFRNSG 243
            A   +L VKG+L+TNPSNPLGTT +  ELN+L  FI ++KN+HL+SDEIYSGT F +S 
Sbjct: 184 QAKKLDLNVKGILITNPSNPLGTTTTQTELNILFDFITKNKNIHLVSDEIYSGTVFNSSE 243

Query: 244 FVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSSFG 303
           F+S MEIL      N  + N+VHIV SLSKDLGLPGFRVGA+YSNDK V++AATKMSSFG
Sbjct: 244 FISVMEILKNNQLENTDVLNRVHIVCSLSKDLGLPGFRVGAIYSNDKDVISAATKMSSFG 303

Query: 304 LVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFCWV 363
           LVSSQTQYLLS++LSDKKF + Y+R+NQKRL+ RQ  LV GLE  GIKC K NAGLFCWV
Sbjct: 304 LVSSQTQYLLSSLLSDKKFTKNYLRENQKRLKNRQRKLVLGLEAIGIKCLKSNAGLFCWV 363

Query: 364 DMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLAMQ 423
           DMR LL   TFE+EM+LW+KI++EV+LN+SPG SCHC EPGWFR+CFANM + TL LA++
Sbjct: 364 DMRPLLRSKTFEAEMDLWKKIVYEVKLNISPGSSCHCEEPGWFRVCFANMIDETLKLALK 423

Query: 424 RLKAFV 429
           RLK  V
Sbjct: 424 RLKMLV 429

BLAST of Csa4G099220 vs. TAIR10
Match: AT4G08040.1 (AT4G08040.1 1-aminocyclopropane-1-carboxylate synthase 11)

HSP 1 Score: 594.0 bits (1530), Expect = 7.8e-170
Identity = 280/428 (65.42%), Postives = 344/428 (80.37%), Query Frame = 1

Query: 3   LLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLEEN 62
           +LS K   + HGQDSSYF GW+EYEKNP+ E  N  GI+QMGLAENQL FD++EKWLEE+
Sbjct: 1   MLSSKVVGDSHGQDSSYFLGWQEYEKNPFHESFNTSGIVQMGLAENQLSFDLIEKWLEEH 60

Query: 63  PNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGATS 122
           P   G K++ +S+FRQLALFQDY+GLPAFK A+A+FM +IR NKV FD N +VLTAG+TS
Sbjct: 61  PEVLGLKKNDESVFRQLALFQDYHGLPAFKDAMAKFMGKIRENKVKFDTNKMVLTAGSTS 120

Query: 123 ANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLEAY 182
           ANETLMFCLA PGDAFL+P PYYPGFDRDLKWRTG+EIVPIHC SSNG+KITE AL +AY
Sbjct: 121 ANETLMFCLANPGDAFLIPAPYYPGFDRDLKWRTGVEIVPIHCVSSNGYKITEDALEDAY 180

Query: 183 QNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRNSG 242
           + AL  NL VKGVL+TNPSNPLGT+ +  EL+LLL F  +K +H++SDEIYSGT F +  
Sbjct: 181 ERALKHNLNVKGVLITNPSNPLGTSTTREELDLLLTFTSTKKIHMVSDEIYSGTVFDSPE 240

Query: 243 FVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSSFG 302
           F S +E+       N  +  ++H+VYSLSKDLGLPGFRVG +YSN++ VV+AATKMSSFG
Sbjct: 241 FTSVLEVAKD---KNMGLDGKIHVVYSLSKDLGLPGFRVGLIYSNNEKVVSAATKMSSFG 300

Query: 303 LVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFCWV 362
           L+SSQTQ+LL+ +LSD++F   Y+ +N+KRL +R+  LV+GL++AGI C K NAGLFCWV
Sbjct: 301 LISSQTQHLLANLLSDERFTTNYLEENKKRLRERKDRLVSGLKEAGISCLKSNAGLFCWV 360

Query: 363 DMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLAMQ 422
           D+RHLL  NTFE+E  LW KI+ EV LN+SPG SCHC EPGWFR+CFANMS+ T+++AM 
Sbjct: 361 DLRHLLKSNTFEAEHSLWTKIVCEVGLNISPGSSCHCDEPGWFRVCFANMSDQTMEVAMD 420

Query: 423 RLKAFVVN 431
           R+K FV N
Sbjct: 421 RVKGFVDN 425

BLAST of Csa4G099220 vs. NCBI nr
Match: gi|449439021|ref|XP_004137286.1| (PREDICTED: 1-aminocyclopropane-1-carboxylate synthase 3-like [Cucumis sativus])

HSP 1 Score: 899.0 bits (2322), Expect = 3.2e-258
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE
Sbjct: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA
Sbjct: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN
Sbjct: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
           SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS
Sbjct: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA
Sbjct: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420

Query: 421 MQRLKAFVVNYSTTITVTSPQM 443
           MQRLKAFVVNYSTTITVTSPQM
Sbjct: 421 MQRLKAFVVNYSTTITVTSPQM 442

BLAST of Csa4G099220 vs. NCBI nr
Match: gi|703109852|ref|XP_010099412.1| (1-aminocyclopropane-1-carboxylate synthase [Morus notabilis])

HSP 1 Score: 706.1 bits (1821), Expect = 4.0e-200
Identity = 335/433 (77.37%), Postives = 391/433 (90.30%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M LLSRKA+CN HGQDSSYF GW+EYEKNPYDE++NPKGIIQMGLAENQL F ++E WL 
Sbjct: 1   MRLLSRKASCNTHGQDSSYFLGWQEYEKNPYDEVKNPKGIIQMGLAENQLSFHLLESWLA 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++AGFKRDGKSIFR+LALFQDY+GLPAFK+AL +FMSEIRGNKV FDPNHIVLTAGA
Sbjct: 61  KNPDAAGFKRDGKSIFRELALFQDYHGLPAFKQALVDFMSEIRGNKVTFDPNHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPG+AFLLPTPYYPGFDRDLKWRTG+EIVPIHC+SSNGF+ITE+AL +
Sbjct: 121 TSANETLMFCLAEPGEAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITEAALNQ 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           A++ A N NL+VKGVLVTNPSNPLGTT++S+E NLL+ FIE+K +HLISDEIYSGT F +
Sbjct: 181 AFEEAENRNLRVKGVLVTNPSNPLGTTMTSNEHNLLVSFIEAKKIHLISDEIYSGTVFTS 240

Query: 241 SGFVSAMEIL-NQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMS 300
            GFVS MEIL N+ Y    ++  +VH+VYSLSKDLGLPGFRVGA+YSND++VVAAATKMS
Sbjct: 241 PGFVSVMEILKNRNYDKFSEVWKRVHVVYSLSKDLGLPGFRVGAIYSNDEMVVAAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLF 360
           SFGLVSSQTQYLLSAMLSD+ F +KYI +NQKRL+KR  MLV+GL+KAG+ C K NAGLF
Sbjct: 301 SFGLVSSQTQYLLSAMLSDRNFTKKYISENQKRLKKRHGMLVSGLQKAGVSCLKSNAGLF 360

Query: 361 CWVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDL 420
           CWVDMRHLL  NTFE+EMELW+KI+++V+LN+SPG SCHC+EPGWFR+CFANMSE TL+L
Sbjct: 361 CWVDMRHLLRSNTFEAEMELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEETLEL 420

Query: 421 AMQRLKAFVVNYS 433
           AMQRLK+FV  Y+
Sbjct: 421 AMQRLKSFVTEYN 433

BLAST of Csa4G099220 vs. NCBI nr
Match: gi|13928421|dbj|BAB47123.1| (ACC synthase [Cucurbita maxima])

HSP 1 Score: 704.5 bits (1817), Expect = 1.2e-199
Identity = 341/386 (88.34%), Postives = 367/386 (95.08%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           MG+LSRKATCN HGQDSSYF GWEEY+KNPYDEI NPKGIIQMGLAENQLCFD++E+WL+
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLK 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           ENP++AGFKRDGKSIFR+LALFQDYNGLPAFKKALAEFMSEIRGN+V F+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGF+ITESALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ+A + +LKVKGVLVTNPSNPLGTTI++ ELNLLLQFI SKNLHLISDEIYSGT FRN
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
            GFVS M+ILNQ YKHNHQI+ QVHIVYSLSKDLGLPGFRVGA+YSND VVVAAATKMSS
Sbjct: 241 PGFVSVMDILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKF + YI++NQKRLEKRQ MLV GL+KAGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFE 387
           WVDMRHLLG NTFESEMELWRKI+FE
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFE 386

BLAST of Csa4G099220 vs. NCBI nr
Match: gi|567867057|ref|XP_006426151.1| (hypothetical protein CICLE_v10027248mg [Citrus clementina])

HSP 1 Score: 697.2 bits (1798), Expect = 1.9e-197
Identity = 337/435 (77.47%), Postives = 382/435 (87.82%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           MGLLS KATCN HGQDSSYF GW+EYEKNPYDE+QNP GIIQMGLAENQL FD++E WL+
Sbjct: 1   MGLLSGKATCNTHGQDSSYFLGWQEYEKNPYDEVQNPTGIIQMGLAENQLSFDLLESWLD 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
            NP++AGFKRDG+SIFR+LALFQDY+GLPAFKKAL EFMSEIRGNKV FD + IVLTAGA
Sbjct: 61  RNPDAAGFKRDGQSIFRELALFQDYHGLPAFKKALVEFMSEIRGNKVTFDTSKIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPG+AFLLPTPYYPGFDRDLKWRTG+EIVPI C+S NGF+ITE AL E
Sbjct: 121 TSANETLMFCLAEPGEAFLLPTPYYPGFDRDLKWRTGVEIVPIQCTSLNGFQITEMALEE 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ A   +LKVKGVLVTNPSNPLGTT+S +ELNLL+ FI  KN+HLISDEIYSGT F +
Sbjct: 181 AYQLAQKRHLKVKGVLVTNPSNPLGTTMSRNELNLLVNFITEKNIHLISDEIYSGTVFSS 240

Query: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300
            GFVS MEIL      N ++ N+VHIVYSLSKDLGLPGFRVGA+YSND  VVAAATKMSS
Sbjct: 241 PGFVSIMEILKDRNCENTEVWNRVHIVYSLSKDLGLPGFRVGAIYSNDDTVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKF + YI +N+KRL++R   LV+GLEKAGI C K NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFAQNYISENKKRLKQRHRKLVSGLEKAGISCLKSNAGLFC 360

Query: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420
           WVDMRHLL  NTFE+EMELW+KI+++V+LN+SPG SCHC+EPGWFR+CFANMSE TL L+
Sbjct: 361 WVDMRHLLSSNTFEAEMELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLKLS 420

Query: 421 MQRLKAFVVNYSTTI 436
           MQRLKAFV + +TT+
Sbjct: 421 MQRLKAFVDSITTTL 435

BLAST of Csa4G099220 vs. NCBI nr
Match: gi|1009145565|ref|XP_015890405.1| (PREDICTED: 1-aminocyclopropane-1-carboxylate synthase-like isoform X1 [Ziziphus jujuba])

HSP 1 Score: 696.0 bits (1795), Expect = 4.1e-197
Identity = 336/436 (77.06%), Postives = 389/436 (89.22%), Query Frame = 1

Query: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60
           M LLSRKATCN HGQDSSYFSGWEEYEKNP+ EI+NPKGIIQMGLAENQL FD +E WL 
Sbjct: 1   MKLLSRKATCNSHGQDSSYFSGWEEYEKNPFHEIRNPKGIIQMGLAENQLSFDFLESWLA 60

Query: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120
           +NP++AGFKRDG+SIFR+LALFQDY+GLPAFKKA  E+M+EIRGNKV+F+P+H+VLTAGA
Sbjct: 61  KNPDAAGFKRDGESIFRELALFQDYHGLPAFKKAFVEYMAEIRGNKVSFNPSHLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180
           TSANETLMFCLAEPG+AFLLPTPYYPGFDRDLKWRTG+EIVPI C+SS+GF+ITE+AL+ 
Sbjct: 121 TSANETLMFCLAEPGEAFLLPTPYYPGFDRDLKWRTGVEIVPIQCTSSDGFQITEAALVA 180

Query: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240
           AYQ+A N NL+VKGVLVTNPSNPLGTT++ +ELNLL+ FIESK +HLISDEIY+GT F +
Sbjct: 181 AYQDAQNRNLRVKGVLVTNPSNPLGTTMTRNELNLLISFIESKGIHLISDEIYAGTVFSS 240

Query: 241 -SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMS 300
            S FVS MEIL    ++   +  +VH+V SLSKDLGLPGFRVGA+YSND +VVAAATKMS
Sbjct: 241 PSDFVSVMEILKA--RNCENVWKRVHLVSSLSKDLGLPGFRVGAIYSNDDIVVAAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLF 360
           SFGLVSSQTQYLLSAMLSDKKF  KYI +NQKRL +R  MLV+GLEKAGIKC K NAGLF
Sbjct: 301 SFGLVSSQTQYLLSAMLSDKKFTNKYISENQKRLRQRHKMLVSGLEKAGIKCLKSNAGLF 360

Query: 361 CWVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDL 420
           CWVDMRHLL  NTFESEMELW++I+++V+LN+SPG SCHC+EPGWFR+CFANMSE TL+L
Sbjct: 361 CWVDMRHLLRSNTFESEMELWKRIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEETLEL 420

Query: 421 AMQRLKAFVVNYSTTI 436
           AMQRL+AFV  YST +
Sbjct: 421 AMQRLRAFVTEYSTVL 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
1A13_SOLLC2.5e-19474.541-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum GN=ACS3 PE=... [more]
1A19_ARATH2.1e-19375.701-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana GN=ACS9 PE=... [more]
1A12_CUCMA2.3e-19274.311-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima GN=ACS2 PE... [more]
1A1C_MALDO1.3e-19073.151-aminocyclopropane-1-carboxylate synthase OS=Malus domestica GN=ACS-1 PE=1 SV=2[more]
1A15_ARATH4.9e-19073.361-aminocyclopropane-1-carboxylate synthase 5 OS=Arabidopsis thaliana GN=ACS5 PE=... [more]
Match NameE-valueIdentityDescription
W9RA06_9ROSA2.8e-20077.371-aminocyclopropane-1-carboxylate synthase OS=Morus notabilis GN=L484_007775 PE=... [more]
V4SQK3_9ROSI1.3e-19777.47Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027248mg PE=3 SV=1[more]
A0A067GGJ6_CITSI3.8e-19777.24Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g043923mg PE=3 SV=1[more]
Q8S935_DIOKA3.9e-19477.161-aminocyclopropane-1-carboxylate synthase OS=Diospyros kaki GN=DK-ACS1 PE=2 SV=... [more]
B2XCJ7_9ROSA6.6e-19475.691-aminocyclopropane 1-carboxylate synthase 1 OS=Prunus salicina GN=ACS1 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
AT3G49700.11.2e-19475.70 1-aminocyclopropane-1-carboxylate synthase 9[more]
AT5G65800.12.7e-19173.36 ACC synthase 5[more]
AT4G37770.17.5e-18971.89 1-amino-cyclopropane-1-carboxylate synthase 8[more]
AT2G22810.18.0e-18371.60 1-aminocyclopropane-1-carboxylate synthase 4[more]
AT4G08040.17.8e-17065.42 1-aminocyclopropane-1-carboxylate synthase 11[more]
Match NameE-valueIdentityDescription
gi|449439021|ref|XP_004137286.1|3.2e-258100.00PREDICTED: 1-aminocyclopropane-1-carboxylate synthase 3-like [Cucumis sativus][more]
gi|703109852|ref|XP_010099412.1|4.0e-20077.371-aminocyclopropane-1-carboxylate synthase [Morus notabilis][more]
gi|13928421|dbj|BAB47123.1|1.2e-19988.34ACC synthase [Cucurbita maxima][more]
gi|567867057|ref|XP_006426151.1|1.9e-19777.47hypothetical protein CICLE_v10027248mg [Citrus clementina][more]
gi|1009145565|ref|XP_015890405.1|4.1e-19777.06PREDICTED: 1-aminocyclopropane-1-carboxylate synthase-like isoform X1 [Ziziphus ... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004838NHTrfase_class1_PyrdxlP-BS
IPR004839Aminotransferase_I/II
IPR015421PyrdxlP-dep_Trfase_major
IPR015422PyrdxlP-dep_Trfase_dom1
IPR015424PyrdxlP-dep_Trfase
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0030170pyridoxal phosphate binding
Vocabulary: Biological Process
TermDefinition
GO:0009058biosynthetic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042218 1-aminocyclopropane-1-carboxylate biosynthetic process
biological_process GO:0006520 cellular amino acid metabolic process
biological_process GO:0009058 biosynthetic process
biological_process GO:0009693 ethylene biosynthetic process
biological_process GO:0009835 fruit ripening
cellular_component GO:0005575 cellular_component
molecular_function GO:0016847 1-aminocyclopropane-1-carboxylate synthase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa4G099220.1Csa4G099220.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004838Aminotransferases, class-I, pyridoxal-phosphate-binding sitePROSITEPS00105AA_TRANSFER_CLASS_1coord: 269..282
scor
IPR004839Aminotransferase, class I/classIIPFAMPF00155Aminotran_1_2coord: 41..424
score: 6.0
IPR015421Pyridoxal phosphate-dependent transferase, major region, subdomain 1GENE3DG3DSA:3.40.640.10coord: 82..313
score: 2.6
IPR015422Pyridoxal phosphate-dependent transferase, major region, subdomain 2GENE3DG3DSA:3.90.1150.10coord: 314..431
score: 1.4
IPR015424Pyridoxal phosphate-dependent transferaseunknownSSF53383PLP-dependent transferasescoord: 8..427
score: 4.98E
NoneNo IPR availablePRINTSPR00753ACCSYNTHASEcoord: 135..156
score: 1.9E-102coord: 113..133
score: 1.9E-102coord: 76..92
score: 1.9E-102coord: 225..248
score: 1.9E-102coord: 295..318
score: 1.9E-102coord: 189..213
score: 1.9E-102coord: 263..287
score: 1.9E-102coord: 43..69
score: 1.9E
NoneNo IPR availablePANTHERPTHR11751SUBGROUP I AMINOTRANSFERASE RELATEDcoord: 18..428
score: 3.9E
NoneNo IPR availablePANTHERPTHR11751:SF4311-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 4-RELATEDcoord: 18..428
score: 3.9E