Csa4G098200 (gene) Cucumber (Chinese Long) v2

NameCsa4G098200
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionPentatricopeptide repeat-containing protein, putative; contains IPR002885 (Pentatricopeptide repeat), IPR004575 (Cdk-activating kinase assembly factor MAT1/Tfb3), IPR011990 (Tetratricopeptide-like helical)
LocationChr4 : 6422596 .. 6423507 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATGTTAAAGCTGGATCATTGGAAGATGCATGCTCAGTTCTTGACTTGATGGCTGAGCAGCAGGACATTGTTCCGGACATATATCTGTTACGGGACATGCTTCGTATTTACCAACGATGTGGCATGGTGCATAAGCTAGCAGATCTGTACTATAGGATATTGAAGAGTGGAGTGTCTTGGGATCAGGAAATGTATAATTGTGTCATAAATTGCTGTTCCCGTGCTCTGCCTGTTGATGAGCTTTCTAGGCTTTTTGATGAAATGCTTCAATGTGGTTTTGCCCCAAATACAGTGACCTTGAATGTCATGCTTGACGTTTATGGGAAATCCAAGCTTTTCACTAAGGCTAGAAATCTCTTTGGGCTTGCTCAGAAAAGAGGTTTGGTTGATGCAATCTCTTATAATACTATGATATCTGTCTATGGGAAGAATAAGGACTTCAAAAACATGTCATCTACGGTTCAGAAAATGAAATTTAACGGGTTTTCAGTTTCCCTTGAAGCCTACAATTGTATGTTGGATGCTTATGGCAAAGAATGCCAAATGGAGAATTTCAGAAGTGTCTTGCAGCGAATGCAGGAGACAAGTTCTGAATGTGACCATTATACGTACAACATCATGATCAACATCTATGGAGAACAAGGATGGATAGATGAAGTTGCGGAAGTTCTGACAGAATTGAAAGCATGTGGACTTGAACCCGATCTGTACAGCTACAACACATTGATCAAGGCATATGGAATAGCAGGGATGGTTGAAGAAGCTGCTCAGTTGGTGAAAGAAATGAGAGAAAAGAGGATAGAACCAGATAGGATAACTTATATTAACATGATTAGAGCACTGCAAAGAAACGATCAATTTTTAGAGGCAGTCAAGTGGTCATTGTGGATGAAGCAGATGAAATATTGA

mRNA sequence

ATGTATGTTAAAGCTGGATCATTGGAAGATGCATGCTCAGTTCTTGACTTGATGGCTGAGCAGCAGGACATTGTTCCGGACATATATCTGTTACGGGACATGCTTCGTATTTACCAACGATGTGGCATGGTGCATAAGCTAGCAGATCTGTACTATAGGATATTGAAGAGTGGAGTGTCTTGGGATCAGGAAATGTATAATTGTGTCATAAATTGCTGTTCCCGTGCTCTGCCTGTTGATGAGCTTTCTAGGCTTTTTGATGAAATGCTTCAATGTGGTTTTGCCCCAAATACAGTGACCTTGAATGTCATGCTTGACGTTTATGGGAAATCCAAGCTTTTCACTAAGGCTAGAAATCTCTTTGGGCTTGCTCAGAAAAGAGGTTTGGTTGATGCAATCTCTTATAATACTATGATATCTGTCTATGGGAAGAATAAGGACTTCAAAAACATGTCATCTACGGTTCAGAAAATGAAATTTAACGGGTTTTCAGTTTCCCTTGAAGCCTACAATTGTATGTTGGATGCTTATGGCAAAGAATGCCAAATGGAGAATTTCAGAAGTGTCTTGCAGCGAATGCAGGAGACAAGTTCTGAATGTGACCATTATACGTACAACATCATGATCAACATCTATGGAGAACAAGGATGGATAGATGAAGTTGCGGAAGTTCTGACAGAATTGAAAGCATGTGGACTTGAACCCGATCTGTACAGCTACAACACATTGATCAAGGCATATGGAATAGCAGGGATGGTTGAAGAAGCTGCTCAGTTGGTGAAAGAAATGAGAGAAAAGAGGATAGAACCAGATAGGATAACTTATATTAACATGATTAGAGCACTGCAAAGAAACGATCAATTTTTAGAGGCAGTCAAGTGGTCATTGTGGATGAAGCAGATGAAATATTGA

Coding sequence (CDS)

ATGTATGTTAAAGCTGGATCATTGGAAGATGCATGCTCAGTTCTTGACTTGATGGCTGAGCAGCAGGACATTGTTCCGGACATATATCTGTTACGGGACATGCTTCGTATTTACCAACGATGTGGCATGGTGCATAAGCTAGCAGATCTGTACTATAGGATATTGAAGAGTGGAGTGTCTTGGGATCAGGAAATGTATAATTGTGTCATAAATTGCTGTTCCCGTGCTCTGCCTGTTGATGAGCTTTCTAGGCTTTTTGATGAAATGCTTCAATGTGGTTTTGCCCCAAATACAGTGACCTTGAATGTCATGCTTGACGTTTATGGGAAATCCAAGCTTTTCACTAAGGCTAGAAATCTCTTTGGGCTTGCTCAGAAAAGAGGTTTGGTTGATGCAATCTCTTATAATACTATGATATCTGTCTATGGGAAGAATAAGGACTTCAAAAACATGTCATCTACGGTTCAGAAAATGAAATTTAACGGGTTTTCAGTTTCCCTTGAAGCCTACAATTGTATGTTGGATGCTTATGGCAAAGAATGCCAAATGGAGAATTTCAGAAGTGTCTTGCAGCGAATGCAGGAGACAAGTTCTGAATGTGACCATTATACGTACAACATCATGATCAACATCTATGGAGAACAAGGATGGATAGATGAAGTTGCGGAAGTTCTGACAGAATTGAAAGCATGTGGACTTGAACCCGATCTGTACAGCTACAACACATTGATCAAGGCATATGGAATAGCAGGGATGGTTGAAGAAGCTGCTCAGTTGGTGAAAGAAATGAGAGAAAAGAGGATAGAACCAGATAGGATAACTTATATTAACATGATTAGAGCACTGCAAAGAAACGATCAATTTTTAGAGGCAGTCAAGTGGTCATTGTGGATGAAGCAGATGAAATATTGA

Protein sequence

MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQMKY*
BLAST of Csa4G098200 vs. Swiss-Prot
Match: PP342_ARATH (Pentatricopeptide repeat-containing protein At4g30825, chloroplastic OS=Arabidopsis thaliana GN=At4g30825 PE=2 SV=2)

HSP 1 Score: 454.1 bits (1167), Expect = 1.2e-126
Identity = 215/301 (71.43%), Postives = 256/301 (85.05%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAGSLE+ACSVL++M EQ+DIVPD+YL RDMLRIYQ+C +  KL  LYYRI KSG+ 
Sbjct: 602 MYVKAGSLEEACSVLEIMDEQKDIVPDVYLFRDMLRIYQKCDLQDKLQHLYYRIRKSGIH 661

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           W+QEMYNCVINCC+RALP+DELS  F+EM++ GF PNTVT NV+LDVYGK+KLF K   L
Sbjct: 662 WNQEMYNCVINCCARALPLDELSGTFEEMIRYGFTPNTVTFNVLLDVYGKAKLFKKVNEL 721

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           F LA++ G+VD ISYNT+I+ YGKNKD+ NMSS ++ M+F+GFSVSLEAYN +LDAYGK+
Sbjct: 722 FLLAKRHGVVDVISYNTIIAAYGKNKDYTNMSSAIKNMQFDGFSVSLEAYNTLLDAYGKD 781

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
            QME FRS+L+RM++++S  DHYTYNIMINIYGEQGWIDEVA+VL ELK  GL PDL SY
Sbjct: 782 KQMEKFRSILKRMKKSTSGPDHYTYNIMINIYGEQGWIDEVADVLKELKESGLGPDLCSY 841

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGI GMVEEA  LVKEMR + I PD++TY N++ AL+RND+FLEA+KWSLWMKQ
Sbjct: 842 NTLIKAYGIGGMVEEAVGLVKEMRGRNIIPDKVTYTNLVTALRRNDEFLEAIKWSLWMKQ 901

Query: 301 M 302
           M
Sbjct: 902 M 902


HSP 2 Score: 97.4 bits (241), Expect = 2.8e-19
Identity = 59/216 (27.31%), Postives = 107/216 (49.54%), Query Frame = 1

Query: 64  EMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGL 123
           +++N VI  C++   V   S+ F  ML+ G  PN  T+ +++ +Y K+    +A   F  
Sbjct: 211 QVFNTVIYACTKKGNVKLASKWFHMMLEFGVRPNVATIGMLMGLYQKNWNVEEAEFAFSH 270

Query: 124 AQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQM 183
            +K G+V   +Y++MI++Y + + +      +  MK +   + LE +  ML+AY ++ +M
Sbjct: 271 MRKFGIVCESAYSSMITIYTRLRLYDKAEEVIDLMKQDRVRLKLENWLVMLNAYSQQGKM 330

Query: 184 ENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTL 243
           E   S+L  M+      +   YN +I  YG+   ++    +   L   GLEPD  SY ++
Sbjct: 331 ELAESILVSMEAAGFSPNIIAYNTLITGYGKIFKMEAAQGLFHRLCNIGLEPDETSYRSM 390

Query: 244 IKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMI 280
           I+ +G A   EEA    +E++    +P+      +I
Sbjct: 391 IEGWGRADNYEEAKHYYQELKRCGYKPNSFNLFTLI 426

BLAST of Csa4G098200 vs. Swiss-Prot
Match: PP362_ARATH (Pentatricopeptide repeat-containing protein At5g02860 OS=Arabidopsis thaliana GN=At5g02860 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.4e-31
Identity = 80/293 (27.30%), Postives = 143/293 (48.81%), Query Frame = 1

Query: 2   YVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMV-HKLADLYYRILKSGVS 61
           +  +G   +A +V   M E+    P +     +L ++ + G   +K+  L  ++   G++
Sbjct: 218 FANSGRYREAVNVFKKM-EEDGCKPTLITYNVILNVFGKMGTPWNKITSLVEKMKSDGIA 277

Query: 62  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 121
            D   YN +I CC R     E +++F+EM   GF+ + VT N +LDVYGKS    +A  +
Sbjct: 278 PDAYTYNTLITCCKRGSLHQEAAQVFEEMKAAGFSYDKVTYNALLDVYGKSHRPKEAMKV 337

Query: 122 FGLAQKRGLVDAI-SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 181
                  G   +I +YN++IS Y ++           +M   G    +  Y  +L  + +
Sbjct: 338 LNEMVLNGFSPSIVTYNSLISAYARDGMLDEAMELKNQMAEKGTKPDVFTYTTLLSGFER 397

Query: 182 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 241
             ++E+  S+ + M+    + +  T+N  I +YG +G   E+ ++  E+  CGL PD+ +
Sbjct: 398 AGKVESAMSIFEEMRNAGCKPNICTFNAFIKMYGNRGKFTEMMKIFDEINVCGLSPDIVT 457

Query: 242 YNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAV 293
           +NTL+  +G  GM  E + + KEM+     P+R T+  +I A  R   F +A+
Sbjct: 458 WNTLLAVFGQNGMDSEVSGVFKEMKRAGFVPERETFNTLISAYSRCGSFEQAM 509


HSP 2 Score: 119.4 bits (298), Expect = 6.8e-26
Identity = 73/254 (28.74%), Postives = 122/254 (48.03%), Query Frame = 1

Query: 42  GMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTL 101
           G V   A+++  + + G S D   Y  +I+  + +    E   +F +M + G  P  +T 
Sbjct: 187 GRVSSAANMFNGLQEDGFSLDVYSYTSLISAFANSGRYREAVNVFKKMEEDGCKPTLITY 246

Query: 102 NVMLDVYGKSKL-FTKARNLFGLAQKRGLV-DAISYNTMISVYGKNKDFKNMSSTVQKMK 161
           NV+L+V+GK    + K  +L    +  G+  DA +YNT+I+   +    +  +   ++MK
Sbjct: 247 NVILNVFGKMGTPWNKITSLVEKMKSDGIAPDAYTYNTLITCCKRGSLHQEAAQVFEEMK 306

Query: 162 FNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWID 221
             GFS     YN +LD YGK  + +    VL  M          TYN +I+ Y   G +D
Sbjct: 307 AAGFSYDKVTYNALLDVYGKSHRPKEAMKVLNEMVLNGFSPSIVTYNSLISAYARDGMLD 366

Query: 222 EVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMI 281
           E  E+  ++   G +PD+++Y TL+  +  AG VE A  + +EMR    +P+  T+   I
Sbjct: 367 EAMELKNQMAEKGTKPDVFTYTTLLSGFERAGKVESAMSIFEEMRNAGCKPNICTFNAFI 426

Query: 282 RALQRNDQFLEAVK 294
           +      +F E +K
Sbjct: 427 KMYGNRGKFTEMMK 440


HSP 3 Score: 114.8 bits (286), Expect = 1.7e-24
Identity = 63/287 (21.95%), Postives = 136/287 (47.39%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           M  K G +  A ++ + + E    + D+Y    ++  +   G   +  +++ ++ + G  
Sbjct: 182 MLGKEGRVSSAANMFNGLQEDGFSL-DVYSYTSLISAFANSGRYREAVNVFKKMEEDGCK 241

Query: 61  WDQEMYNCVINCCSR-ALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARN 120
                YN ++N   +   P ++++ L ++M   G AP+  T N ++    +  L  +A  
Sbjct: 242 PTLITYNVILNVFGKMGTPWNKITSLVEKMKSDGIAPDAYTYNTLITCCKRGSLHQEAAQ 301

Query: 121 LFGLAQKRGL-VDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYG 180
           +F   +  G   D ++YN ++ VYGK+   K     + +M  NGFS S+  YN ++ AY 
Sbjct: 302 VFEEMKAAGFSYDKVTYNALLDVYGKSHRPKEAMKVLNEMVLNGFSPSIVTYNSLISAYA 361

Query: 181 KECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLY 240
           ++  ++    +  +M E  ++ D +TY  +++ +   G ++    +  E++  G +P++ 
Sbjct: 362 RDGMLDEAMELKNQMAEKGTKPDVFTYTTLLSGFERAGKVESAMSIFEEMRNAGCKPNIC 421

Query: 241 SYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRN 286
           ++N  IK YG  G   E  ++  E+    + PD +T+  ++    +N
Sbjct: 422 TFNAFIKMYGNRGKFTEMMKIFDEINVCGLSPDIVTWNTLLAVFGQN 467


HSP 4 Score: 109.4 bits (272), Expect = 7.1e-23
Identity = 74/273 (27.11%), Postives = 123/273 (45.05%), Query Frame = 1

Query: 25  VPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVDELSR 84
           VP+      ++  Y RCG   +   +Y R+L +GV+ D   YN V+   +R    ++  +
Sbjct: 486 VPERETFNTLISAYSRCGSFEQAMTVYRRMLDAGVTPDLSTYNTVLAALARGGMWEQSEK 545

Query: 85  LFDEMLQCGFAPNTVTLNVMLDVYGKSK----LFTKARNLF-GLAQKRGLVDAISYNTMI 144
           +  EM      PN +T   +L  Y   K    + + A  ++ G+ + R    A+   T++
Sbjct: 546 VLAEMEDGRCKPNELTYCSLLHAYANGKEIGLMHSLAEEVYSGVIEPR----AVLLKTLV 605

Query: 145 SVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSE 204
            V  K            ++K  GFS  +   N M+  YG+   +     VL  M+E    
Sbjct: 606 LVCSKCDLLPEAERAFSELKERGFSPDITTLNSMVSIYGRRQMVAKANGVLDYMKERGFT 665

Query: 205 CDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQL 264
               TYN ++ ++       +  E+L E+ A G++PD+ SYNT+I AY     + +A+++
Sbjct: 666 PSMATYNSLMYMHSRSADFGKSEEILREILAKGIKPDIISYNTVIYAYCRNTRMRDASRI 725

Query: 265 VKEMREKRIEPDRITYINMIRALQRNDQFLEAV 293
             EMR   I PD ITY   I +   +  F EA+
Sbjct: 726 FSEMRNSGIVPDVITYNTFIGSYAADSMFEEAI 754


HSP 5 Score: 107.1 bits (266), Expect = 3.5e-22
Identity = 74/292 (25.34%), Postives = 133/292 (45.55%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           +Y K+   ++A  VL+ M       P I     ++  Y R GM+ +  +L  ++ + G  
Sbjct: 323 VYGKSHRPKEAMKVLNEMV-LNGFSPSIVTYNSLISAYARDGMLDEAMELKNQMAEKGTK 382

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
            D   Y  +++   RA  V+    +F+EM   G  PN  T N  + +YG    FT+   +
Sbjct: 383 PDVFTYTTLLSGFERAGKVESAMSIFEEMRNAGCKPNICTFNAFIKMYGNRGKFTEMMKI 442

Query: 121 FGLAQKRGL-VDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 180
           F      GL  D +++NT+++V+G+N     +S   ++MK  GF    E +N ++ AY +
Sbjct: 443 FDEINVCGLSPDIVTWNTLLAVFGQNGMDSEVSGVFKEMKRAGFVPERETFNTLISAYSR 502

Query: 181 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 240
               E   +V +RM +     D  TYN ++      G  ++  +VL E++    +P+  +
Sbjct: 503 CGSFEQAMTVYRRMLDAGVTPDLSTYNTVLAALARGGMWEQSEKVLAEMEDGRCKPNELT 562

Query: 241 YNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEA 292
           Y +L+ AY     +     L +E+    IEP  +    ++    + D   EA
Sbjct: 563 YCSLLHAYANGKEIGLMHSLAEEVYSGVIEPRAVLLKTLVLVCSKCDLLPEA 613


HSP 6 Score: 96.7 bits (239), Expect = 4.8e-19
Identity = 61/255 (23.92%), Postives = 122/255 (47.84%), Query Frame = 1

Query: 17  LMAEQQD--IVPDIYLLRDMLRIY---QRCGMVHKLADLYYRILKSGVSWDQE-MYNCVI 76
           ++AE +D    P+      +L  Y   +  G++H LA+  Y    SGV   +  +   ++
Sbjct: 546 VLAEMEDGRCKPNELTYCSLLHAYANGKEIGLMHSLAEEVY----SGVIEPRAVLLKTLV 605

Query: 77  NCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLV 136
             CS+   + E  R F E+ + GF+P+  TLN M+ +YG+ ++  KA  +    ++RG  
Sbjct: 606 LVCSKCDLLPEAERAFSELKERGFSPDITTLNSMVSIYGRRQMVAKANGVLDYMKERGFT 665

Query: 137 DAI-SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSV 196
            ++ +YN+++ ++ ++ DF      ++++   G    + +YN ++ AY +  +M +   +
Sbjct: 666 PSMATYNSLMYMHSRSADFGKSEEILREILAKGIKPDIISYNTVIYAYCRNTRMRDASRI 725

Query: 197 LQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGI 256
              M+ +    D  TYN  I  Y      +E   V+  +   G  P+  +YN+++  Y  
Sbjct: 726 FSEMRNSGIVPDVITYNTFIGSYAADSMFEEAIGVVRYMIKHGCRPNQNTYNSIVDGYCK 785

Query: 257 AGMVEEAAQLVKEMR 265
               +EA   V+++R
Sbjct: 786 LNRKDEAKLFVEDLR 796


HSP 7 Score: 95.1 bits (235), Expect = 1.4e-18
Identity = 65/280 (23.21%), Postives = 125/280 (44.64%), Query Frame = 1

Query: 4   KAGSL-EDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWD 63
           K GSL ++A  V + M +      D      +L +Y +     +   +   ++ +G S  
Sbjct: 290 KRGSLHQEAAQVFEEM-KAAGFSYDKVTYNALLDVYGKSHRPKEAMKVLNEMVLNGFSPS 349

Query: 64  QEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFG 123
              YN +I+  +R   +DE   L ++M + G  P+  T   +L  + ++     A ++F 
Sbjct: 350 IVTYNSLISAYARDGMLDEAMELKNQMAEKGTKPDVFTYTTLLSGFERAGKVESAMSIFE 409

Query: 124 LAQKRGLVDAI-SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKEC 183
             +  G    I ++N  I +YG    F  M     ++   G S  +  +N +L  +G+  
Sbjct: 410 EMRNAGCKPNICTFNAFIKMYGNRGKFTEMMKIFDEINVCGLSPDIVTWNTLLAVFGQNG 469

Query: 184 QMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYN 243
                  V + M+      +  T+N +I+ Y   G  ++   V   +   G+ PDL +YN
Sbjct: 470 MDSEVSGVFKEMKRAGFVPERETFNTLISAYSRCGSFEQAMTVYRRMLDAGVTPDLSTYN 529

Query: 244 TLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRA 282
           T++ A    GM E++ +++ EM + R +P+ +TY +++ A
Sbjct: 530 TVLAALARGGMWEQSEKVLAEMEDGRCKPNELTYCSLLHA 568


HSP 8 Score: 84.0 bits (206), Expect = 3.2e-15
Identity = 60/288 (20.83%), Postives = 123/288 (42.71%), Query Frame = 1

Query: 2   YVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSW 61
           + +AG +E A S+ + M       P+I      +++Y   G   ++  ++  I   G+S 
Sbjct: 394 FERAGKVESAMSIFEEM-RNAGCKPNICTFNAFIKMYGNRGKFTEMMKIFDEINVCGLSP 453

Query: 62  DQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLF 121
           D   +N ++    +     E+S +F EM + GF P   T N ++  Y +   F +A  ++
Sbjct: 454 DIVTWNTLLAVFGQNGMDSEVSGVFKEMKRAGFVPERETFNTLISAYSRCGSFEQAMTVY 513

Query: 122 GLAQKRGLV-DAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 181
                 G+  D  +YNT+++   +   ++     + +M+      +   Y  +L AY   
Sbjct: 514 RRMLDAGVTPDLSTYNTVLAALARGGMWEQSEKVLAEMEDGRCKPNELTYCSLLHAYANG 573

Query: 182 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 241
            ++    S+ + +     E        ++ +  +   + E     +ELK  G  PD+ + 
Sbjct: 574 KEIGLMHSLAEEVYSGVIEPRAVLLKTLVLVCSKCDLLPEAERAFSELKERGFSPDITTL 633

Query: 242 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQF 289
           N+++  YG   MV +A  ++  M+E+   P   TY +++    R+  F
Sbjct: 634 NSMVSIYGRRQMVAKANGVLDYMKERGFTPSMATYNSLMYMHSRSADF 680

BLAST of Csa4G098200 vs. Swiss-Prot
Match: PP413_ARATH (Pentatricopeptide repeat-containing protein At5g42310, mitochondrial OS=Arabidopsis thaliana GN=At5g42310 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.2e-30
Identity = 84/288 (29.17%), Postives = 142/288 (49.31%), Query Frame = 1

Query: 2   YVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSW 61
           YV AG  E A  VL  M E  D+ P+ ++   +L  ++  G   K   +   +   GV  
Sbjct: 384 YVNAGRWESARIVLKEM-EAGDVQPNSFVFSRLLAGFRDRGEWQKTFQVLKEMKSIGVKP 443

Query: 62  DQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLF 121
           D++ YN VI+   +   +D     FD ML  G  P+ VT N ++D + K      A  +F
Sbjct: 444 DRQFYNVVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCHCKHGRHIVAEEMF 503

Query: 122 GLAQKRGLVD-AISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 181
              ++RG +  A +YN MI+ YG  + + +M   + KMK  G   ++  +  ++D YGK 
Sbjct: 504 EAMERRGCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNVVTHTTLVDVYGKS 563

Query: 182 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 241
            +  +    L+ M+    +     YN +IN Y ++G  ++       + + GL+P L + 
Sbjct: 564 GRFNDAIECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNAFRVMTSDGLKPSLLAL 623

Query: 242 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQF 289
           N+LI A+G      EA  +++ M+E  ++PD +TY  +++AL R D+F
Sbjct: 624 NSLINAFGEDRRDAEAFAVLQYMKENGVKPDVVTYTTLMKALIRVDKF 670


HSP 2 Score: 95.5 bits (236), Expect = 1.1e-18
Identity = 64/269 (23.79%), Postives = 122/269 (45.35%), Query Frame = 1

Query: 24  IVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVDELS 83
           + PD      ++  + +   +      + R+L  G+  D+  +N +I+C  +        
Sbjct: 440 VKPDRQFYNVVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCHCKHGRHIVAE 499

Query: 84  RLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLV-DAISYNTMISVY 143
            +F+ M + G  P   T N+M++ YG  + +   + L G  + +G++ + +++ T++ VY
Sbjct: 500 EMFEAMERRGCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNVVTHTTLVDVY 559

Query: 144 GKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSECDH 203
           GK+  F +    +++MK  G   S   YN +++AY +    E   +  + M     +   
Sbjct: 560 GKSGRFNDAIECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNAFRVMTSDGLKPSL 619

Query: 204 YTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQLVKE 263
              N +IN +GE     E   VL  +K  G++PD+ +Y TL+KA       ++   + +E
Sbjct: 620 LALNSLINAFGEDRRDAEAFAVLQYMKENGVKPDVVTYTTLMKALIRVDKFQKVPVVYEE 679

Query: 264 MREKRIEPDRITYINMIRALQRNDQFLEA 292
           M     +PDR     +  AL+   Q L A
Sbjct: 680 MIMSGCKPDRKARSMLRSALRYMKQTLRA 708


HSP 3 Score: 84.0 bits (206), Expect = 3.2e-15
Identity = 61/262 (23.28%), Postives = 126/262 (48.09%), Query Frame = 1

Query: 17  LMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRA 76
           L++++Q + P  Y    ++    R   + K  +L  ++ + G   D   Y+ VI   +R+
Sbjct: 188 LLSQKQTLTPLTY--NALIGACARNNDIEKALNLIAKMRQDGYQSDFVNYSLVIQSLTRS 247

Query: 77  LPVDE--LSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGL-VDAI 136
             +D   L RL+ E+ +     +   +N ++  + KS   +KA  L G+AQ  GL     
Sbjct: 248 NKIDSVMLLRLYKEIERDKLELDVQLVNDIIMGFAKSGDPSKALQLLGMAQATGLSAKTA 307

Query: 137 SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRM 196
           +  ++IS    +       +  ++++ +G      AYN +L  Y K   +++  S++  M
Sbjct: 308 TLVSIISALADSGRTLEAEALFEELRQSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEM 367

Query: 197 QETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMV 256
           ++     D +TY+++I+ Y   G  +    VL E++A  ++P+ + ++ L+  +   G  
Sbjct: 368 EKRGVSPDEHTYSLLIDAYVNAGRWESARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEW 427

Query: 257 EEAAQLVKEMREKRIEPDRITY 276
           ++  Q++KEM+   ++PDR  Y
Sbjct: 428 QKTFQVLKEMKSIGVKPDRQFY 447


HSP 4 Score: 83.6 bits (205), Expect = 4.2e-15
Identity = 63/273 (23.08%), Postives = 118/273 (43.22%), Query Frame = 1

Query: 21  QQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVD 80
           Q  I P       +L+ Y + G +     +   + K GVS D+  Y+ +I+    A   +
Sbjct: 332 QSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEMEKRGVSPDEHTYSLLIDAYVNAGRWE 391

Query: 81  ELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLV-DAISYNTMI 140
               +  EM      PN+   + +L  +     + K   +    +  G+  D   YN +I
Sbjct: 392 SARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEWQKTFQVLKEMKSIGVKPDRQFYNVVI 451

Query: 141 SVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSE 200
             +GK     +  +T  +M   G       +N ++D + K  +      + + M+     
Sbjct: 452 DTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCHCKHGRHIVAEEMFEAMERRGCL 511

Query: 201 CDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQL 260
               TYNIMIN YG+Q   D++  +L ++K+ G+ P++ ++ TL+  YG +G   +A + 
Sbjct: 512 PCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNVVTHTTLVDVYGKSGRFNDAIEC 571

Query: 261 VKEMREKRIEPDRITYINMIRALQRNDQFLEAV 293
           ++EM+   ++P    Y  +I A  +     +AV
Sbjct: 572 LEEMKSVGLKPSSTMYNALINAYAQRGLSEQAV 604


HSP 5 Score: 60.1 bits (144), Expect = 4.9e-08
Identity = 45/240 (18.75%), Postives = 105/240 (43.75%), Query Frame = 1

Query: 66  YNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKAR--NLFGL 125
           YN +I  C+R   +++   L  +M Q G+  + V  ++++    +S          L+  
Sbjct: 200 YNALIGACARNNDIEKALNLIAKMRQDGYQSDFVNYSLVIQSLTRSNKIDSVMLLRLYKE 259

Query: 126 AQKRGL-VDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQ 185
            ++  L +D    N +I  + K+ D       +   +  G S        ++ A     +
Sbjct: 260 IERDKLELDVQLVNDIIMGFAKSGDPSKALQLLGMAQATGLSAKTATLVSIISALADSGR 319

Query: 186 MENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNT 245
                ++ + ++++  +     YN ++  Y + G + +   +++E++  G+ PD ++Y+ 
Sbjct: 320 TLEAEALFEELRQSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEMEKRGVSPDEHTYSL 379

Query: 246 LIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQMK 303
           LI AY  AG  E A  ++KEM    ++P+   +  ++   +   ++ +  +    +K+MK
Sbjct: 380 LIDAYVNAGRWESARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEWQKTFQ---VLKEMK 436

BLAST of Csa4G098200 vs. Swiss-Prot
Match: PP408_ARATH (Pentatricopeptide repeat-containing protein At5g39980, chloroplastic OS=Arabidopsis thaliana GN=At5g39980 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 3.3e-28
Identity = 81/282 (28.72%), Postives = 142/282 (50.35%), Query Frame = 1

Query: 4   KAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQ 63
           K G  + A S L  M EQ  +  D+ L  +++ + +R     K   ++ R+ +SG++ D 
Sbjct: 202 KEGMFDSALSWLQKM-EQDRVSGDLVLYSNLIELSRRLCDYSKAISIFSRLKRSGITPDL 261

Query: 64  EMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFG- 123
             YN +IN   +A    E   L  EM + G  PNTV+ + +L VY ++  F +A ++F  
Sbjct: 262 VAYNSMINVYGKAKLFREARLLIKEMNEAGVLPNTVSYSTLLSVYVENHKFLEALSVFAE 321

Query: 124 LAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQ 183
           + +    +D  + N MI VYG+    K        ++      ++ +YN +L  YG+   
Sbjct: 322 MKEVNCALDLTTCNIMIDVYGQLDMVKEADRLFWSLRKMDIEPNVVSYNTILRVYGEAEL 381

Query: 184 MENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNT 243
                 + + MQ    E +  TYN MI IYG+    ++   ++ E+++ G+EP+  +Y+T
Sbjct: 382 FGEAIHLFRLMQRKDIEQNVVTYNTMIKIYGKTMEHEKATNLVQEMQSRGIEPNAITYST 441

Query: 244 LIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQR 285
           +I  +G AG ++ AA L +++R   +E D++ Y  MI A +R
Sbjct: 442 IISIWGKAGKLDRAATLFQKLRSSGVEIDQVLYQTMIVAYER 482


HSP 2 Score: 126.7 bits (317), Expect = 4.3e-28
Identity = 78/278 (28.06%), Postives = 140/278 (50.36%), Query Frame = 1

Query: 3   VKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWD 62
           ++A   + A  + D M  Q+ + PD Y    ++  + + GM         ++ +  VS D
Sbjct: 166 LRAKQFDIAHGLFDEM-RQRALAPDRYTYSTLITSFGKEGMFDSALSWLQKMEQDRVSGD 225

Query: 63  QEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFG 122
             +Y+ +I    R     +   +F  + + G  P+ V  N M++VYGK+KLF +AR L  
Sbjct: 226 LVLYSNLIELSRRLCDYSKAISIFSRLKRSGITPDLVAYNSMINVYGKAKLFREARLLIK 285

Query: 123 LAQKRGLV-DAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKEC 182
              + G++ + +SY+T++SVY +N  F    S   +MK    ++ L   N M+D YG+  
Sbjct: 286 EMNEAGVLPNTVSYSTLLSVYVENHKFLEALSVFAEMKEVNCALDLTTCNIMIDVYGQLD 345

Query: 183 QMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYN 242
            ++    +   +++   E +  +YN ++ +YGE     E   +   ++   +E ++ +YN
Sbjct: 346 MVKEADRLFWSLRKMDIEPNVVSYNTILRVYGEAELFGEAIHLFRLMQRKDIEQNVVTYN 405

Query: 243 TLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMI 280
           T+IK YG     E+A  LV+EM+ + IEP+ ITY  +I
Sbjct: 406 TMIKIYGKTMEHEKATNLVQEMQSRGIEPNAITYSTII 442


HSP 3 Score: 110.9 bits (276), Expect = 2.4e-23
Identity = 73/273 (26.74%), Postives = 133/273 (48.72%), Query Frame = 1

Query: 20  EQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPV 79
           ++  I PD+     M+ +Y +  +  +   L   + ++GV  +   Y+ +++        
Sbjct: 252 KRSGITPDLVAYNSMINVYGKAKLFREARLLIKEMNEAGVLPNTVSYSTLLSVYVENHKF 311

Query: 80  DELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLV-DAISYNTM 139
            E   +F EM +   A +  T N+M+DVYG+  +  +A  LF   +K  +  + +SYNT+
Sbjct: 312 LEALSVFAEMKEVNCALDLTTCNIMIDVYGQLDMVKEADRLFWSLRKMDIEPNVVSYNTI 371

Query: 140 ISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSS 199
           + VYG+ + F       + M+      ++  YN M+  YGK  + E   +++Q MQ    
Sbjct: 372 LRVYGEAELFGEAIHLFRLMQRKDIEQNVVTYNTMIKIYGKTMEHEKATNLVQEMQSRGI 431

Query: 200 ECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQ 259
           E +  TY+ +I+I+G+ G +D  A +  +L++ G+E D   Y T+I AY   G++  A +
Sbjct: 432 EPNAITYSTIISIWGKAGKLDRAATLFQKLRSSGVEIDQVLYQTMIVAYERVGLMGHAKR 491

Query: 260 LVKEMREKRIEPDRITYINMIRALQRNDQFLEA 292
           L+ E++     PD I     I  L +  +  EA
Sbjct: 492 LLHELK----LPDNIPRETAITILAKAGRTEEA 520


HSP 4 Score: 107.5 bits (267), Expect = 2.7e-22
Identity = 74/294 (25.17%), Postives = 138/294 (46.94%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           +Y KA    +A  ++  M E   ++P+      +L +Y       +   ++  + +   +
Sbjct: 269 VYGKAKLFREARLLIKEMNEA-GVLPNTVSYSTLLSVYVENHKFLEALSVFAEMKEVNCA 328

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
            D    N +I+   +   V E  RLF  + +    PN V+ N +L VYG+++LF +A +L
Sbjct: 329 LDLTTCNIMIDVYGQLDMVKEADRLFWSLRKMDIEPNVVSYNTILRVYGEAELFGEAIHL 388

Query: 121 FGLAQKRGL-VDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 180
           F L Q++ +  + ++YNTMI +YGK  + +  ++ VQ+M+  G   +   Y+ ++  +GK
Sbjct: 389 FRLMQRKDIEQNVVTYNTMIKIYGKTMEHEKATNLVQEMQSRGIEPNAITYSTIISIWGK 448

Query: 181 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 240
             +++   ++ Q+++ +  E D   Y  MI  Y   G +     +L ELK     PD   
Sbjct: 449 AGKLDRAATLFQKLRSSGVEIDQVLYQTMIVAYERVGLMGHAKRLLHELKL----PDNIP 508

Query: 241 YNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVK 294
             T I     AG  EEA  + ++  E     D   +  MI    RN +++  ++
Sbjct: 509 RETAITILAKAGRTEEATWVFRQAFESGEVKDISVFGCMINLYSRNQRYVNVIE 557


HSP 5 Score: 79.7 bits (195), Expect = 6.0e-14
Identity = 44/168 (26.19%), Postives = 82/168 (48.81%), Query Frame = 1

Query: 134 SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRM 193
           +YN ++    + K F        +M+    +     Y+ ++ ++GKE   ++  S LQ+M
Sbjct: 157 AYNVVLRNVLRAKQFDIAHGLFDEMRQRALAPDRYTYSTLITSFGKEGMFDSALSWLQKM 216

Query: 194 QETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMV 253
           ++     D   Y+ +I +        +   + + LK  G+ PDL +YN++I  YG A + 
Sbjct: 217 EQDRVSGDLVLYSNLIELSRRLCDYSKAISIFSRLKRSGITPDLVAYNSMINVYGKAKLF 276

Query: 254 EEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQM 302
            EA  L+KEM E  + P+ ++Y  ++     N +FLEA+     MK++
Sbjct: 277 REARLLIKEMNEAGVLPNTVSYSTLLSVYVENHKFLEALSVFAEMKEV 324


HSP 6 Score: 75.5 bits (184), Expect = 1.1e-12
Identity = 62/272 (22.79%), Postives = 125/272 (45.96%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           +Y K    E A +++  M + + I P+      ++ I+ + G + + A L+ ++  SGV 
Sbjct: 409 IYGKTMEHEKATNLVQEM-QSRGIEPNAITYSTIISIWGKAGKLDRAATLFQKLRSSGVE 468

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
            DQ +Y  +I    R   +    RL  E+      P+ +     + +  K+    +A  +
Sbjct: 469 IDQVLYQTMIVAYERVGLMGHAKRLLHELK----LPDNIPRETAITILAKAGRTEEATWV 528

Query: 121 FGLAQKRGLVDAIS-YNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 180
           F  A + G V  IS +  MI++Y +N+ + N+    +KM+  G+         +L+AYGK
Sbjct: 529 FRQAFESGEVKDISVFGCMINLYSRNQRYVNVIEVFEKMRTAGYFPDSNVIAMVLNAYGK 588

Query: 181 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 240
           + + E   +V + MQE         +  M+++Y  +   + V  +   L++   +P++ S
Sbjct: 589 QREFEKADTVYREMQEEGCVFPDEVHFQMLSLYSSKKDFEMVESLFQRLES---DPNVNS 648

Query: 241 ---YNTLIKAYGIAGMVEEAAQLVKEMREKRI 269
              +  +   Y  A  + +A++++  MRE+ I
Sbjct: 649 KELHLVVAALYERADKLNDASRVMNRMRERGI 672

BLAST of Csa4G098200 vs. Swiss-Prot
Match: PP241_ARATH (Pentatricopeptide repeat-containing protein At3g18110, chloroplastic OS=Arabidopsis thaliana GN=EMB1270 PE=2 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-26
Identity = 82/301 (27.24%), Postives = 148/301 (49.17%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCG-MVHKLA-DLYYRILKSG 60
           +Y ++G    A  ++D M  Q+  VPD+     ++    + G +   LA +L   +  SG
Sbjct: 234 VYSRSGKFSKAQELVDAM-RQRGCVPDLISFNTLINARLKSGGLTPNLAVELLDMVRNSG 293

Query: 61  VSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKAR 120
           +  D   YN +++ CSR   +D   ++F++M      P+  T N M+ VYG+  L  +A 
Sbjct: 294 LRPDAITYNTLLSACSRDSNLDGAVKVFEDMEAHRCQPDLWTYNAMISVYGRCGLAAEAE 353

Query: 121 NLFGLAQKRGLV-DAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAY 180
            LF   + +G   DA++YN+++  + + ++ + +    Q+M+  GF      YN ++  Y
Sbjct: 354 RLFMELELKGFFPDAVTYNSLLYAFARERNTEKVKEVYQQMQKMGFGKDEMTYNTIIHMY 413

Query: 181 GKECQMENFRSVLQRMQETSS-ECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPD 240
           GK+ Q++    + + M+  S    D  TY ++I+  G+     E A +++E+   G++P 
Sbjct: 414 GKQGQLDLALQLYKDMKGLSGRNPDAITYTVLIDSLGKANRTVEAAALMSEMLDVGIKPT 473

Query: 241 LYSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSL 298
           L +Y+ LI  Y  AG  EEA      M     +PD + Y  M+  L R ++  +A  W L
Sbjct: 474 LQTYSALICGYAKAGKREEAEDTFSCMLRSGTKPDNLAYSVMLDVLLRGNETRKA--WGL 531


HSP 2 Score: 102.8 bits (255), Expect = 6.6e-21
Identity = 70/280 (25.00%), Postives = 131/280 (46.79%), Query Frame = 1

Query: 21   QQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVD 80
            Q    PD+     ++  Y +CG   +   ++  +++ G S   E  N +++       ++
Sbjct: 780  QSGRTPDLKTWNSLMSAYAQCGCYERARAIFNTMMRDGPSPTVESINILLHALCVDGRLE 839

Query: 81   ELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLVDAIS-YNTMI 140
            EL  + +E+   GF  +  ++ +MLD + ++    + + ++   +  G +  I  Y  MI
Sbjct: 840  ELYVVVEELQDMGFKISKSSILLMLDAFARAGNIFEVKKIYSSMKAAGYLPTIRLYRMMI 899

Query: 141  SVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSE 200
             +  K K  ++    V +M+   F V L  +N ML  Y      +    V QR++ET  E
Sbjct: 900  ELLCKGKRVRDAEIMVSEMEEANFKVELAIWNSMLKMYTAIEDYKKTVQVYQRIKETGLE 959

Query: 201  CDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQL 260
             D  TYN +I +Y      +E   ++ +++  GL+P L +Y +LI A+G    +E+A QL
Sbjct: 960  PDETTYNTLIIMYCRDRRPEEGYLLMQQMRNLGLDPKLDTYKSLISAFGKQKCLEQAEQL 1019

Query: 261  VKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMK 300
             +E+  K ++ DR  Y  M++  + +    +A K    MK
Sbjct: 1020 FEELLSKGLKLDRSFYHTMMKISRDSGSDSKAEKLLQMMK 1059

BLAST of Csa4G098200 vs. TrEMBL
Match: A0A0A0LTR9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G257890 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 3.4e-173
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS
Sbjct: 592 MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 651

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL
Sbjct: 652 WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 711

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE
Sbjct: 712 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 771

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
           CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY
Sbjct: 772 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 831

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ
Sbjct: 832 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 891

Query: 301 MKY 304
           MKY
Sbjct: 892 MKY 894

BLAST of Csa4G098200 vs. TrEMBL
Match: A0A0A0LTR9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G257890 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 9.0e-17
Identity = 56/229 (24.45%), Postives = 112/229 (48.91%), Query Frame = 1

Query: 58  GVSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKA 117
           G   D +++N +I  C ++  V++ ++ F  ML+C   PN  T  +++ +Y K     ++
Sbjct: 195 GSQLDFQVFNTLIYACYKSRFVEQGTKWFRMMLECQVQPNVATFGMLMGLYQKKCDIKES 254

Query: 118 RNLFGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAY 177
              F   +  G+V   +Y +MI++Y +   +      +Q M+ +    +LE +  ML+AY
Sbjct: 255 EFAFNQMRNFGIVCETAYASMITIYIRMNLYDKAEEVIQLMQEDKVIPNLENWVVMLNAY 314

Query: 178 GKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDL 237
            ++ +ME    V   M+E     +   YN +I  YG+   +D    +   +K  G+EPD 
Sbjct: 315 CQQGKMEEAELVFASMEEAGFSSNIIAYNTLITGYGKASNMDTAQRLFLGIKNSGVEPDE 374

Query: 238 YSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRND 287
            +Y ++I+ +G AG  + A    KE++ +   P+      +I    +++
Sbjct: 375 TTYRSMIEGWGRAGNYKMAEWYYKELKRRGYMPNSSNLFTLINLQAKHE 423


HSP 2 Score: 67.4 bits (163), Expect = 3.4e-08
Identity = 64/286 (22.38%), Postives = 132/286 (46.15%), Query Frame = 1

Query: 2   YVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKS-GVS 61
           Y KA ++ D    L L  +   + PD    R M+  + R G  +K+A+ YY+ LK  G  
Sbjct: 349 YGKASNM-DTAQRLFLGIKNSGVEPDETTYRSMIEGWGRAGN-YKMAEWYYKELKRRGYM 408

Query: 62  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 121
            +      +IN  ++        +  ++ML+ G  P+++  NV L  Y K++       L
Sbjct: 409 PNSSNLFTLINLQAKHEDEAGTLKTLNDMLKIGCRPSSIVGNV-LQAYEKARRMKSVPVL 468

Query: 122 F-GLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 181
             G   ++ L    S + ++  Y K+    +    +++ ++         Y+ ++ +  +
Sbjct: 469 LTGSFYRKVLSSQTSCSILVMAYVKHCLVDDALKVLREKEWKDHHFEENLYHLLICSCKE 528

Query: 182 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 241
              +EN   +  ++ +  ++ + +    MI+IY   G   +  ++   L++ G+  DL +
Sbjct: 529 LGHLENAIKIYTQLPKRENKPNLHITCTMIDIYSIMGRFSDGEKLYLSLRSSGIPLDLIA 588

Query: 242 YNTLIKAYGIAGMVEEAAQLVKEMREKR-IEPDRITYINMIRALQR 285
           YN +++ Y  AG +E+A  ++  M E++ I PD     +M+R  QR
Sbjct: 589 YNVVVRMYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQR 631


HSP 3 Score: 57.0 bits (136), Expect = 4.6e-05
Identity = 62/300 (20.67%), Postives = 135/300 (45.00%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           +Y++    + A  V+ LM E + ++P++     ML  Y + G + +   ++  + ++G S
Sbjct: 278 IYIRMNLYDKAEEVIQLMQEDK-VIPNLENWVVMLNAYCQQGKMEEAELVFASMEEAGFS 337

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
            +   YN +I    +A  +D   RLF  +   G  P+  T   M++ +G++  +  A   
Sbjct: 338 SNIIAYNTLITGYGKASNMDTAQRLFLGIKNSGVEPDETTYRSMIEGWGRAGNYKMAEWY 397

Query: 121 FGLAQKRG-LVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 180
           +   ++RG + ++ +  T+I++  K++D      T+  M   G   S    N +L AY K
Sbjct: 398 YKELKRRGYMPNSSNLFTLINLQAKHEDEAGTLKTLNDMLKIGCRPSSIVGN-VLQAYEK 457

Query: 181 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 240
             +M++   +L             + +I++  Y +   +D+  +VL E +      +   
Sbjct: 458 ARRMKSVPVLLTGSFYRKVLSSQTSCSILVMAYVKHCLVDDALKVLREKEWKDHHFEENL 517

Query: 241 YNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMK 300
           Y+ LI +    G +E A ++  ++ ++  +P+      MI       +F +  K  L ++
Sbjct: 518 YHLLICSCKELGHLENAIKIYTQLPKRENKPNLHITCTMIDIYSIMGRFSDGEKLYLSLR 575


HSP 4 Score: 494.6 bits (1272), Expect = 8.7e-137
Identity = 237/301 (78.74%), Postives = 266/301 (88.37%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAG+LEDACSVLD M +Q+ IVPDIY+ RDMLRIYQRCG + KL DLYY++LKSGV+
Sbjct: 464 MYVKAGALEDACSVLDTMDKQEGIVPDIYMFRDMLRIYQRCGRLDKLKDLYYKLLKSGVT 523

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           WDQEMYNCVINCCSRALPVDE+S +FDEMLQCGF PNT+T NVMLDVYGK+KL  KAR L
Sbjct: 524 WDQEMYNCVINCCSRALPVDEISEIFDEMLQCGFVPNTITFNVMLDVYGKAKLLKKARKL 583

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           F +AQK GLVD ISYNT+I+ YG+NKD +NMSST  +M+F GFSVSLEAYN MLDAYGKE
Sbjct: 584 FWMAQKWGLVDMISYNTIIAAYGRNKDLRNMSSTFGEMQFKGFSVSLEAYNTMLDAYGKE 643

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
            QME FRSVLQRM+ETS   DHYTYNIMINIYGEQGWIDEVA+VLTELK CGL PDL SY
Sbjct: 644 SQMERFRSVLQRMKETSCASDHYTYNIMINIYGEQGWIDEVADVLTELKECGLGPDLCSY 703

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGIAGMVE+A  LVKEMRE  I+PD+ITYIN+I AL++ND++LEAVKWSLWMKQ
Sbjct: 704 NTLIKAYGIAGMVEDAVHLVKEMRENGIQPDKITYINLINALRKNDEYLEAVKWSLWMKQ 763

Query: 301 M 302
           M
Sbjct: 764 M 764

BLAST of Csa4G098200 vs. TrEMBL
Match: M5VU43_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa019391mg PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 4.6e-13
Identity = 53/236 (22.46%), Postives = 114/236 (48.31%), Query Frame = 1

Query: 58  GVSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKA 117
           G   + +++N +I  C +   ++   + F  ML+    PN  T  +++ +Y K     +A
Sbjct: 67  GCELNYQVFNTLIYACCKLGRLELGGKWFRMMLEHEVQPNIATFGMLMVLYQKGWNVEEA 126

Query: 118 RNLFGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAY 177
              F   +  G++   +Y++MI++Y +   F+     +  +K +   ++L+ +  M++AY
Sbjct: 127 EFTFFQMRNFGILCQSAYSSMITIYTRLNLFEKAEEIIGLLKEDRVRLNLDNWLVMINAY 186

Query: 178 GKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDL 237
            ++ ++++   VL  MQE     +   YN +I  YG+   +D    +   +K  GLEPD 
Sbjct: 187 CQQGKVDDAELVLVSMQEAGFSPNIIAYNTLITGYGKASKMDAADHLFQGIKNAGLEPDE 246

Query: 238 YSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVK 294
            +Y ++I+ +G A    EA    KE++    +P+      +I    +++    A++
Sbjct: 247 TTYRSMIEGWGRADNYMEAEWYYKELKRLGYKPNSSNLYTLINLQAKHEDEEGAIR 302


HSP 2 Score: 82.0 bits (201), Expect = 1.3e-12
Identity = 63/273 (23.08%), Postives = 120/273 (43.96%), Query Frame = 1

Query: 33  DMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVDELSRLFDEML-Q 92
           D+L+  +RC  V  L    +      +  +   +N V+    R    D   +L  E++  
Sbjct: 6   DILKRLERCSDVKTLRFFEWMRSNGKLERNVSAFNLVLRVMGRREDWDGAEKLVQEVIAD 65

Query: 93  CGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLVDAIS-YNTMISVYGKNKDFKN 152
            G   N    N ++    K          F +  +  +   I+ +  ++ +Y K  + + 
Sbjct: 66  LGCELNYQVFNTLIYACCKLGRLELGGKWFRMMLEHEVQPNIATFGMLMVLYQKGWNVEE 125

Query: 153 MSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSECDHYTYNIMIN 212
              T  +M+  G  +   AY+ M+  Y +    E    ++  ++E     +   + +MIN
Sbjct: 126 AEFTFFQMRNFGI-LCQSAYSSMITIYTRLNLFEKAEEIIGLLKEDRVRLNLDNWLVMIN 185

Query: 213 IYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEP 272
            Y +QG +D+   VL  ++  G  P++ +YNTLI  YG A  ++ A  L + ++   +EP
Sbjct: 186 AYCQQGKVDDAELVLVSMQEAGFSPNIIAYNTLITGYGKASKMDAADHLFQGIKNAGLEP 245

Query: 273 DRITYINMIRALQRNDQFLEAVKWSLWMKQMKY 304
           D  TY +MI    R D ++EA  +   +K++ Y
Sbjct: 246 DETTYRSMIEGWGRADNYMEAEWYYKELKRLGY 277


HSP 3 Score: 82.0 bits (201), Expect = 1.3e-12
Identity = 57/247 (23.08%), Postives = 120/247 (48.58%), Query Frame = 1

Query: 45  HKLADLYYRILKSGV----SWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVT 104
           H L D   ++L+  +     ++  +Y+ +I  C     ++   +++ +M +    PN   
Sbjct: 363 HCLVDDTMKVLREKLWKDPPFEDNLYHLLICSCKELGHLENAVKIYKQMPRYDDKPNMHI 422

Query: 105 LNVMLDVYGKSKLFTKARNLFGLAQKRGL-VDAISYNTMISVYGKNKDFKNMSSTVQKM- 164
           +  M+D+Y    LFT+A  ++   +  G+ +D I+Y+  + +Y K    ++  S +  M 
Sbjct: 423 MCTMIDIYIIMGLFTEAEKIYVELKSSGVALDMIAYSIAVRMYVKAGALEDACSVLDTMD 482

Query: 165 KFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWI 224
           K  G    +  +  ML  Y +  +++  + +  ++ ++    D   YN +IN       +
Sbjct: 483 KQEGIVPDIYMFRDMLRIYQRCGRLDKLKDLYYKLLKSGVTWDQEMYNCVINCCSRALPV 542

Query: 225 DEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINM 284
           DE++E+  E+  CG  P+  ++N ++  YG A ++++A +L   M +K    D I+Y  +
Sbjct: 543 DEISEIFDEMLQCGFVPNTITFNVMLDVYGKAKLLKKARKLF-WMAQKWGLVDMISYNTI 602

Query: 285 IRALQRN 286
           I A  RN
Sbjct: 603 IAAYGRN 608


HSP 4 Score: 481.5 bits (1238), Expect = 7.6e-133
Identity = 234/301 (77.74%), Postives = 268/301 (89.04%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVK+GSL+DACSVL+ M EQ++IVPDIYL  DMLRIYQ+CGM+ KL DLYYRILK+GV+
Sbjct: 598 MYVKSGSLKDACSVLETMDEQKNIVPDIYLFCDMLRIYQQCGMLDKLQDLYYRILKTGVT 657

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           WD EMYNCVINCC+RALPVDELSRLFDEML  GFAPNT+TLNVMLDVYGKS+LF KAR +
Sbjct: 658 WDSEMYNCVINCCARALPVDELSRLFDEMLLHGFAPNTITLNVMLDVYGKSRLFKKARKV 717

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
             LA+KRGLVD ISYNT+I+ YG++KD K M STV++M+FNGFSVSLE YNCMLD+YGKE
Sbjct: 718 LWLARKRGLVDVISYNTIIAAYGQSKDLKKMLSTVRQMQFNGFSVSLEVYNCMLDSYGKE 777

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
            Q+E+FRSVL+RM+E+S   DHYTYNIMINIYGEQGWI+EVA VLTELK  GL PDL SY
Sbjct: 778 GQIESFRSVLRRMKESSCASDHYTYNIMINIYGEQGWIEEVANVLTELKESGLGPDLCSY 837

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGIAGMVE+A  LVKEMRE  I+PDRITYIN+I AL++ND+FLEAVKWSLWMKQ
Sbjct: 838 NTLIKAYGIAGMVEDAVVLVKEMRENGIQPDRITYINLINALRKNDEFLEAVKWSLWMKQ 897

Query: 301 M 302
           M
Sbjct: 898 M 898

BLAST of Csa4G098200 vs. TrEMBL
Match: D7U7F9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0013g02120 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.2e-14
Identity = 52/216 (24.07%), Postives = 103/216 (47.69%), Query Frame = 1

Query: 64  EMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGL 123
           ++YN +I  C +   V+  ++ F  ML+ G  PN  T  +++ +Y K      +   F  
Sbjct: 209 QVYNTLIYACYKQGHVELGTKWFRLMLENGVRPNVATFGMVMSLYQKGWNVADSEYAFSQ 268

Query: 124 AQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQM 183
            +  G+    +Y+ MI++Y +   +      +  ++ +   ++LE +  +L+AY ++ ++
Sbjct: 269 MRSFGITCQSAYSAMITIYTRMSLYDKAEEVIDFIQEDKVILNLENWLVLLNAYSQQGKL 328

Query: 184 ENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTL 243
           +    VL  MQ      +   YN++I  YG+   +D    +   LK  GLEPD  +Y ++
Sbjct: 329 QEAERVLFSMQNAGFSPNIVAYNMLITGYGKASNMDAAQHIFRNLKNVGLEPDESTYRSM 388

Query: 244 IKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMI 280
           I+ +G A   +EA     E++    +P+      MI
Sbjct: 389 IEGWGRAENYKEAEWYYNELKRLGFKPNSSNLYTMI 424


HSP 2 Score: 478.8 bits (1231), Expect = 4.9e-132
Identity = 229/302 (75.83%), Postives = 264/302 (87.42%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAGSL+DACS L +M +Q+DIVPDIYL RDMLRIYQ+C M  KL  LYYRILKSG++
Sbjct: 609 MYVKAGSLKDACSALQMMEKQKDIVPDIYLFRDMLRIYQKCNMQEKLTTLYYRILKSGIT 668

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           WDQEMYNCVINCC+RALPVDELS++F+ ML  GFAPNT+T NVMLDVYGK+KLF K + L
Sbjct: 669 WDQEMYNCVINCCARALPVDELSKIFNRMLHHGFAPNTITFNVMLDVYGKAKLFRKVKKL 728

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           F +A+  GLVD ISYNT+IS YG+NKDFKNMSST+++M+FNGFSVSLEAYNCMLDAYGKE
Sbjct: 729 FWMAKTGGLVDVISYNTIISAYGQNKDFKNMSSTIREMQFNGFSVSLEAYNCMLDAYGKE 788

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
            +ME FRSVLQRM+E++   DHYTYNIMINIYGE+ WIDEVA VLTELK CG+ PDL SY
Sbjct: 789 GEMEKFRSVLQRMKESNCASDHYTYNIMINIYGERRWIDEVAAVLTELKECGVGPDLCSY 848

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGIAGMVE+A  L+KEMR   IEPDRITY N+I AL++ND+FLEAVKWSLWMKQ
Sbjct: 849 NTLIKAYGIAGMVEDAVGLIKEMRGNGIEPDRITYSNLITALRKNDKFLEAVKWSLWMKQ 908

Query: 301 MK 303
           M+
Sbjct: 909 ME 910

BLAST of Csa4G098200 vs. TrEMBL
Match: A0A0D2MWJ6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G107200 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 6.9e-17
Identity = 60/217 (27.65%), Postives = 109/217 (50.23%), Query Frame = 1

Query: 57  SGVSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTK 116
           SG   D +++N +I  CS+   V+  ++ F  ML+ G  PN  T  +++ +Y K      
Sbjct: 211 SGCELDFQVFNTIIYACSKRGIVEMGAKWFRMMLEHGVQPNVATYGMLMGLYQKGWNVRD 270

Query: 117 ARNLFGLAQKR--GLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCML 176
           A   F L+Q R  G+V   +Y+ MI++Y +   +      +  M+ +  +++LE +  ML
Sbjct: 271 AE--FALSQMRSSGIVCQSAYSAMITIYTRLSLYDKAEEVISFMREDKVALNLENWLVML 330

Query: 177 DAYGKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLE 236
           +AY +  +++    VL  MQE     +   YN +I  YG    +D    V   ++  GLE
Sbjct: 331 NAYSQSGKLDEAEQVLVSMQEAGFSPNIVAYNTLITGYGRASNMDAAQLVFLSIRQVGLE 390

Query: 237 PDLYSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPD 272
           PD  +Y ++I+ +G  G  +EA    + M++   +P+
Sbjct: 391 PDGTTYRSMIEGWGRTGNYKEAGWYYRAMKQLGFKPN 425


HSP 2 Score: 83.2 bits (204), Expect = 6.1e-13
Identity = 46/170 (27.06%), Postives = 86/170 (50.59%), Query Frame = 1

Query: 134 SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRM 193
           +Y  ++ +Y K  + ++    + +M+ +G  V   AY+ M+  Y +    +    V+  M
Sbjct: 254 TYGMLMGLYQKGWNVRDAEFALSQMRSSGI-VCQSAYSAMITIYTRLSLYDKAEEVISFM 313

Query: 194 QETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMV 253
           +E     +   + +M+N Y + G +DE  +VL  ++  G  P++ +YNTLI  YG A  +
Sbjct: 314 REDKVALNLENWLVMLNAYSQSGKLDEAEQVLVSMQEAGFSPNIVAYNTLITGYGRASNM 373

Query: 254 EEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQMKY 304
           + A  +   +R+  +EPD  TY +MI    R   + EA  +   MKQ+ +
Sbjct: 374 DAAQLVFLSIRQVGLEPDGTTYRSMIEGWGRTGNYKEAGWYYRAMKQLGF 422


HSP 3 Score: 478.8 bits (1231), Expect = 4.9e-132
Identity = 229/302 (75.83%), Postives = 264/302 (87.42%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAGSL+DACS L +M +Q+DIVPDIYL RDMLRIYQ+C M  KL  LYYRILKSG++
Sbjct: 562 MYVKAGSLKDACSALQMMEKQKDIVPDIYLFRDMLRIYQKCNMQEKLTTLYYRILKSGIT 621

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           WDQEMYNCVINCC+RALPVDELS++F+ ML  GFAPNT+T NVMLDVYGK+KLF K + L
Sbjct: 622 WDQEMYNCVINCCARALPVDELSKIFNRMLHHGFAPNTITFNVMLDVYGKAKLFRKVKKL 681

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           F +A+  GLVD ISYNT+IS YG+NKDFKNMSST+++M+FNGFSVSLEAYNCMLDAYGKE
Sbjct: 682 FWMAKTGGLVDVISYNTIISAYGQNKDFKNMSSTIREMQFNGFSVSLEAYNCMLDAYGKE 741

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
            +ME FRSVLQRM+E++   DHYTYNIMINIYGE+ WIDEVA VLTELK CG+ PDL SY
Sbjct: 742 GEMEKFRSVLQRMKESNCASDHYTYNIMINIYGERRWIDEVAAVLTELKECGVGPDLCSY 801

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGIAGMVE+A  L+KEMR   IEPDRITY N+I AL++ND+FLEAVKWSLWMKQ
Sbjct: 802 NTLIKAYGIAGMVEDAVGLIKEMRGNGIEPDRITYSNLITALRKNDKFLEAVKWSLWMKQ 861

Query: 301 MK 303
           M+
Sbjct: 862 ME 863

BLAST of Csa4G098200 vs. TAIR10
Match: AT4G30825.1 (AT4G30825.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 454.1 bits (1167), Expect = 6.6e-128
Identity = 215/301 (71.43%), Postives = 256/301 (85.05%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAGSLE+ACSVL++M EQ+DIVPD+YL RDMLRIYQ+C +  KL  LYYRI KSG+ 
Sbjct: 602 MYVKAGSLEEACSVLEIMDEQKDIVPDVYLFRDMLRIYQKCDLQDKLQHLYYRIRKSGIH 661

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           W+QEMYNCVINCC+RALP+DELS  F+EM++ GF PNTVT NV+LDVYGK+KLF K   L
Sbjct: 662 WNQEMYNCVINCCARALPLDELSGTFEEMIRYGFTPNTVTFNVLLDVYGKAKLFKKVNEL 721

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           F LA++ G+VD ISYNT+I+ YGKNKD+ NMSS ++ M+F+GFSVSLEAYN +LDAYGK+
Sbjct: 722 FLLAKRHGVVDVISYNTIIAAYGKNKDYTNMSSAIKNMQFDGFSVSLEAYNTLLDAYGKD 781

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
            QME FRS+L+RM++++S  DHYTYNIMINIYGEQGWIDEVA+VL ELK  GL PDL SY
Sbjct: 782 KQMEKFRSILKRMKKSTSGPDHYTYNIMINIYGEQGWIDEVADVLKELKESGLGPDLCSY 841

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGI GMVEEA  LVKEMR + I PD++TY N++ AL+RND+FLEA+KWSLWMKQ
Sbjct: 842 NTLIKAYGIGGMVEEAVGLVKEMRGRNIIPDKVTYTNLVTALRRNDEFLEAIKWSLWMKQ 901

Query: 301 M 302
           M
Sbjct: 902 M 902


HSP 2 Score: 97.4 bits (241), Expect = 1.6e-20
Identity = 59/216 (27.31%), Postives = 107/216 (49.54%), Query Frame = 1

Query: 64  EMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGL 123
           +++N VI  C++   V   S+ F  ML+ G  PN  T+ +++ +Y K+    +A   F  
Sbjct: 211 QVFNTVIYACTKKGNVKLASKWFHMMLEFGVRPNVATIGMLMGLYQKNWNVEEAEFAFSH 270

Query: 124 AQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQM 183
            +K G+V   +Y++MI++Y + + +      +  MK +   + LE +  ML+AY ++ +M
Sbjct: 271 MRKFGIVCESAYSSMITIYTRLRLYDKAEEVIDLMKQDRVRLKLENWLVMLNAYSQQGKM 330

Query: 184 ENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTL 243
           E   S+L  M+      +   YN +I  YG+   ++    +   L   GLEPD  SY ++
Sbjct: 331 ELAESILVSMEAAGFSPNIIAYNTLITGYGKIFKMEAAQGLFHRLCNIGLEPDETSYRSM 390

Query: 244 IKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMI 280
           I+ +G A   EEA    +E++    +P+      +I
Sbjct: 391 IEGWGRADNYEEAKHYYQELKRCGYKPNSFNLFTLI 426

BLAST of Csa4G098200 vs. TAIR10
Match: AT5G02860.1 (AT5G02860.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 138.3 bits (347), Expect = 8.0e-33
Identity = 80/293 (27.30%), Postives = 143/293 (48.81%), Query Frame = 1

Query: 2   YVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMV-HKLADLYYRILKSGVS 61
           +  +G   +A +V   M E+    P +     +L ++ + G   +K+  L  ++   G++
Sbjct: 218 FANSGRYREAVNVFKKM-EEDGCKPTLITYNVILNVFGKMGTPWNKITSLVEKMKSDGIA 277

Query: 62  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 121
            D   YN +I CC R     E +++F+EM   GF+ + VT N +LDVYGKS    +A  +
Sbjct: 278 PDAYTYNTLITCCKRGSLHQEAAQVFEEMKAAGFSYDKVTYNALLDVYGKSHRPKEAMKV 337

Query: 122 FGLAQKRGLVDAI-SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 181
                  G   +I +YN++IS Y ++           +M   G    +  Y  +L  + +
Sbjct: 338 LNEMVLNGFSPSIVTYNSLISAYARDGMLDEAMELKNQMAEKGTKPDVFTYTTLLSGFER 397

Query: 182 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 241
             ++E+  S+ + M+    + +  T+N  I +YG +G   E+ ++  E+  CGL PD+ +
Sbjct: 398 AGKVESAMSIFEEMRNAGCKPNICTFNAFIKMYGNRGKFTEMMKIFDEINVCGLSPDIVT 457

Query: 242 YNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAV 293
           +NTL+  +G  GM  E + + KEM+     P+R T+  +I A  R   F +A+
Sbjct: 458 WNTLLAVFGQNGMDSEVSGVFKEMKRAGFVPERETFNTLISAYSRCGSFEQAM 509


HSP 2 Score: 119.4 bits (298), Expect = 3.9e-27
Identity = 73/254 (28.74%), Postives = 122/254 (48.03%), Query Frame = 1

Query: 42  GMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTL 101
           G V   A+++  + + G S D   Y  +I+  + +    E   +F +M + G  P  +T 
Sbjct: 187 GRVSSAANMFNGLQEDGFSLDVYSYTSLISAFANSGRYREAVNVFKKMEEDGCKPTLITY 246

Query: 102 NVMLDVYGKSKL-FTKARNLFGLAQKRGLV-DAISYNTMISVYGKNKDFKNMSSTVQKMK 161
           NV+L+V+GK    + K  +L    +  G+  DA +YNT+I+   +    +  +   ++MK
Sbjct: 247 NVILNVFGKMGTPWNKITSLVEKMKSDGIAPDAYTYNTLITCCKRGSLHQEAAQVFEEMK 306

Query: 162 FNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWID 221
             GFS     YN +LD YGK  + +    VL  M          TYN +I+ Y   G +D
Sbjct: 307 AAGFSYDKVTYNALLDVYGKSHRPKEAMKVLNEMVLNGFSPSIVTYNSLISAYARDGMLD 366

Query: 222 EVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMI 281
           E  E+  ++   G +PD+++Y TL+  +  AG VE A  + +EMR    +P+  T+   I
Sbjct: 367 EAMELKNQMAEKGTKPDVFTYTTLLSGFERAGKVESAMSIFEEMRNAGCKPNICTFNAFI 426

Query: 282 RALQRNDQFLEAVK 294
           +      +F E +K
Sbjct: 427 KMYGNRGKFTEMMK 440


HSP 3 Score: 114.8 bits (286), Expect = 9.5e-26
Identity = 63/287 (21.95%), Postives = 136/287 (47.39%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           M  K G +  A ++ + + E    + D+Y    ++  +   G   +  +++ ++ + G  
Sbjct: 182 MLGKEGRVSSAANMFNGLQEDGFSL-DVYSYTSLISAFANSGRYREAVNVFKKMEEDGCK 241

Query: 61  WDQEMYNCVINCCSR-ALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARN 120
                YN ++N   +   P ++++ L ++M   G AP+  T N ++    +  L  +A  
Sbjct: 242 PTLITYNVILNVFGKMGTPWNKITSLVEKMKSDGIAPDAYTYNTLITCCKRGSLHQEAAQ 301

Query: 121 LFGLAQKRGL-VDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYG 180
           +F   +  G   D ++YN ++ VYGK+   K     + +M  NGFS S+  YN ++ AY 
Sbjct: 302 VFEEMKAAGFSYDKVTYNALLDVYGKSHRPKEAMKVLNEMVLNGFSPSIVTYNSLISAYA 361

Query: 181 KECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLY 240
           ++  ++    +  +M E  ++ D +TY  +++ +   G ++    +  E++  G +P++ 
Sbjct: 362 RDGMLDEAMELKNQMAEKGTKPDVFTYTTLLSGFERAGKVESAMSIFEEMRNAGCKPNIC 421

Query: 241 SYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRN 286
           ++N  IK YG  G   E  ++  E+    + PD +T+  ++    +N
Sbjct: 422 TFNAFIKMYGNRGKFTEMMKIFDEINVCGLSPDIVTWNTLLAVFGQN 467


HSP 4 Score: 109.4 bits (272), Expect = 4.0e-24
Identity = 74/273 (27.11%), Postives = 123/273 (45.05%), Query Frame = 1

Query: 25  VPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVDELSR 84
           VP+      ++  Y RCG   +   +Y R+L +GV+ D   YN V+   +R    ++  +
Sbjct: 486 VPERETFNTLISAYSRCGSFEQAMTVYRRMLDAGVTPDLSTYNTVLAALARGGMWEQSEK 545

Query: 85  LFDEMLQCGFAPNTVTLNVMLDVYGKSK----LFTKARNLF-GLAQKRGLVDAISYNTMI 144
           +  EM      PN +T   +L  Y   K    + + A  ++ G+ + R    A+   T++
Sbjct: 546 VLAEMEDGRCKPNELTYCSLLHAYANGKEIGLMHSLAEEVYSGVIEPR----AVLLKTLV 605

Query: 145 SVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSE 204
            V  K            ++K  GFS  +   N M+  YG+   +     VL  M+E    
Sbjct: 606 LVCSKCDLLPEAERAFSELKERGFSPDITTLNSMVSIYGRRQMVAKANGVLDYMKERGFT 665

Query: 205 CDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQL 264
               TYN ++ ++       +  E+L E+ A G++PD+ SYNT+I AY     + +A+++
Sbjct: 666 PSMATYNSLMYMHSRSADFGKSEEILREILAKGIKPDIISYNTVIYAYCRNTRMRDASRI 725

Query: 265 VKEMREKRIEPDRITYINMIRALQRNDQFLEAV 293
             EMR   I PD ITY   I +   +  F EA+
Sbjct: 726 FSEMRNSGIVPDVITYNTFIGSYAADSMFEEAI 754


HSP 5 Score: 107.1 bits (266), Expect = 2.0e-23
Identity = 74/292 (25.34%), Postives = 133/292 (45.55%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           +Y K+   ++A  VL+ M       P I     ++  Y R GM+ +  +L  ++ + G  
Sbjct: 323 VYGKSHRPKEAMKVLNEMV-LNGFSPSIVTYNSLISAYARDGMLDEAMELKNQMAEKGTK 382

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
            D   Y  +++   RA  V+    +F+EM   G  PN  T N  + +YG    FT+   +
Sbjct: 383 PDVFTYTTLLSGFERAGKVESAMSIFEEMRNAGCKPNICTFNAFIKMYGNRGKFTEMMKI 442

Query: 121 FGLAQKRGL-VDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 180
           F      GL  D +++NT+++V+G+N     +S   ++MK  GF    E +N ++ AY +
Sbjct: 443 FDEINVCGLSPDIVTWNTLLAVFGQNGMDSEVSGVFKEMKRAGFVPERETFNTLISAYSR 502

Query: 181 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 240
               E   +V +RM +     D  TYN ++      G  ++  +VL E++    +P+  +
Sbjct: 503 CGSFEQAMTVYRRMLDAGVTPDLSTYNTVLAALARGGMWEQSEKVLAEMEDGRCKPNELT 562

Query: 241 YNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEA 292
           Y +L+ AY     +     L +E+    IEP  +    ++    + D   EA
Sbjct: 563 YCSLLHAYANGKEIGLMHSLAEEVYSGVIEPRAVLLKTLVLVCSKCDLLPEA 613


HSP 6 Score: 96.7 bits (239), Expect = 2.7e-20
Identity = 61/255 (23.92%), Postives = 122/255 (47.84%), Query Frame = 1

Query: 17  LMAEQQD--IVPDIYLLRDMLRIY---QRCGMVHKLADLYYRILKSGVSWDQE-MYNCVI 76
           ++AE +D    P+      +L  Y   +  G++H LA+  Y    SGV   +  +   ++
Sbjct: 546 VLAEMEDGRCKPNELTYCSLLHAYANGKEIGLMHSLAEEVY----SGVIEPRAVLLKTLV 605

Query: 77  NCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLV 136
             CS+   + E  R F E+ + GF+P+  TLN M+ +YG+ ++  KA  +    ++RG  
Sbjct: 606 LVCSKCDLLPEAERAFSELKERGFSPDITTLNSMVSIYGRRQMVAKANGVLDYMKERGFT 665

Query: 137 DAI-SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSV 196
            ++ +YN+++ ++ ++ DF      ++++   G    + +YN ++ AY +  +M +   +
Sbjct: 666 PSMATYNSLMYMHSRSADFGKSEEILREILAKGIKPDIISYNTVIYAYCRNTRMRDASRI 725

Query: 197 LQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGI 256
              M+ +    D  TYN  I  Y      +E   V+  +   G  P+  +YN+++  Y  
Sbjct: 726 FSEMRNSGIVPDVITYNTFIGSYAADSMFEEAIGVVRYMIKHGCRPNQNTYNSIVDGYCK 785

Query: 257 AGMVEEAAQLVKEMR 265
               +EA   V+++R
Sbjct: 786 LNRKDEAKLFVEDLR 796


HSP 7 Score: 95.1 bits (235), Expect = 7.8e-20
Identity = 65/280 (23.21%), Postives = 125/280 (44.64%), Query Frame = 1

Query: 4   KAGSL-EDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWD 63
           K GSL ++A  V + M +      D      +L +Y +     +   +   ++ +G S  
Sbjct: 290 KRGSLHQEAAQVFEEM-KAAGFSYDKVTYNALLDVYGKSHRPKEAMKVLNEMVLNGFSPS 349

Query: 64  QEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFG 123
              YN +I+  +R   +DE   L ++M + G  P+  T   +L  + ++     A ++F 
Sbjct: 350 IVTYNSLISAYARDGMLDEAMELKNQMAEKGTKPDVFTYTTLLSGFERAGKVESAMSIFE 409

Query: 124 LAQKRGLVDAI-SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKEC 183
             +  G    I ++N  I +YG    F  M     ++   G S  +  +N +L  +G+  
Sbjct: 410 EMRNAGCKPNICTFNAFIKMYGNRGKFTEMMKIFDEINVCGLSPDIVTWNTLLAVFGQNG 469

Query: 184 QMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYN 243
                  V + M+      +  T+N +I+ Y   G  ++   V   +   G+ PDL +YN
Sbjct: 470 MDSEVSGVFKEMKRAGFVPERETFNTLISAYSRCGSFEQAMTVYRRMLDAGVTPDLSTYN 529

Query: 244 TLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRA 282
           T++ A    GM E++ +++ EM + R +P+ +TY +++ A
Sbjct: 530 TVLAALARGGMWEQSEKVLAEMEDGRCKPNELTYCSLLHA 568


HSP 8 Score: 84.0 bits (206), Expect = 1.8e-16
Identity = 60/288 (20.83%), Postives = 123/288 (42.71%), Query Frame = 1

Query: 2   YVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSW 61
           + +AG +E A S+ + M       P+I      +++Y   G   ++  ++  I   G+S 
Sbjct: 394 FERAGKVESAMSIFEEM-RNAGCKPNICTFNAFIKMYGNRGKFTEMMKIFDEINVCGLSP 453

Query: 62  DQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLF 121
           D   +N ++    +     E+S +F EM + GF P   T N ++  Y +   F +A  ++
Sbjct: 454 DIVTWNTLLAVFGQNGMDSEVSGVFKEMKRAGFVPERETFNTLISAYSRCGSFEQAMTVY 513

Query: 122 GLAQKRGLV-DAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 181
                 G+  D  +YNT+++   +   ++     + +M+      +   Y  +L AY   
Sbjct: 514 RRMLDAGVTPDLSTYNTVLAALARGGMWEQSEKVLAEMEDGRCKPNELTYCSLLHAYANG 573

Query: 182 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 241
            ++    S+ + +     E        ++ +  +   + E     +ELK  G  PD+ + 
Sbjct: 574 KEIGLMHSLAEEVYSGVIEPRAVLLKTLVLVCSKCDLLPEAERAFSELKERGFSPDITTL 633

Query: 242 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQF 289
           N+++  YG   MV +A  ++  M+E+   P   TY +++    R+  F
Sbjct: 634 NSMVSIYGRRQMVAKANGVLDYMKERGFTPSMATYNSLMYMHSRSADF 680

BLAST of Csa4G098200 vs. TAIR10
Match: AT5G42310.1 (AT5G42310.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 135.2 bits (339), Expect = 6.8e-32
Identity = 84/288 (29.17%), Postives = 142/288 (49.31%), Query Frame = 1

Query: 2   YVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSW 61
           YV AG  E A  VL  M E  D+ P+ ++   +L  ++  G   K   +   +   GV  
Sbjct: 384 YVNAGRWESARIVLKEM-EAGDVQPNSFVFSRLLAGFRDRGEWQKTFQVLKEMKSIGVKP 443

Query: 62  DQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLF 121
           D++ YN VI+   +   +D     FD ML  G  P+ VT N ++D + K      A  +F
Sbjct: 444 DRQFYNVVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCHCKHGRHIVAEEMF 503

Query: 122 GLAQKRGLVD-AISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 181
              ++RG +  A +YN MI+ YG  + + +M   + KMK  G   ++  +  ++D YGK 
Sbjct: 504 EAMERRGCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNVVTHTTLVDVYGKS 563

Query: 182 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 241
            +  +    L+ M+    +     YN +IN Y ++G  ++       + + GL+P L + 
Sbjct: 564 GRFNDAIECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNAFRVMTSDGLKPSLLAL 623

Query: 242 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQF 289
           N+LI A+G      EA  +++ M+E  ++PD +TY  +++AL R D+F
Sbjct: 624 NSLINAFGEDRRDAEAFAVLQYMKENGVKPDVVTYTTLMKALIRVDKF 670


HSP 2 Score: 95.5 bits (236), Expect = 6.0e-20
Identity = 64/269 (23.79%), Postives = 122/269 (45.35%), Query Frame = 1

Query: 24  IVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVDELS 83
           + PD      ++  + +   +      + R+L  G+  D+  +N +I+C  +        
Sbjct: 440 VKPDRQFYNVVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCHCKHGRHIVAE 499

Query: 84  RLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLV-DAISYNTMISVY 143
            +F+ M + G  P   T N+M++ YG  + +   + L G  + +G++ + +++ T++ VY
Sbjct: 500 EMFEAMERRGCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNVVTHTTLVDVY 559

Query: 144 GKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSECDH 203
           GK+  F +    +++MK  G   S   YN +++AY +    E   +  + M     +   
Sbjct: 560 GKSGRFNDAIECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNAFRVMTSDGLKPSL 619

Query: 204 YTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQLVKE 263
              N +IN +GE     E   VL  +K  G++PD+ +Y TL+KA       ++   + +E
Sbjct: 620 LALNSLINAFGEDRRDAEAFAVLQYMKENGVKPDVVTYTTLMKALIRVDKFQKVPVVYEE 679

Query: 264 MREKRIEPDRITYINMIRALQRNDQFLEA 292
           M     +PDR     +  AL+   Q L A
Sbjct: 680 MIMSGCKPDRKARSMLRSALRYMKQTLRA 708


HSP 3 Score: 84.0 bits (206), Expect = 1.8e-16
Identity = 61/262 (23.28%), Postives = 126/262 (48.09%), Query Frame = 1

Query: 17  LMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRA 76
           L++++Q + P  Y    ++    R   + K  +L  ++ + G   D   Y+ VI   +R+
Sbjct: 188 LLSQKQTLTPLTY--NALIGACARNNDIEKALNLIAKMRQDGYQSDFVNYSLVIQSLTRS 247

Query: 77  LPVDE--LSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGL-VDAI 136
             +D   L RL+ E+ +     +   +N ++  + KS   +KA  L G+AQ  GL     
Sbjct: 248 NKIDSVMLLRLYKEIERDKLELDVQLVNDIIMGFAKSGDPSKALQLLGMAQATGLSAKTA 307

Query: 137 SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRM 196
           +  ++IS    +       +  ++++ +G      AYN +L  Y K   +++  S++  M
Sbjct: 308 TLVSIISALADSGRTLEAEALFEELRQSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEM 367

Query: 197 QETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMV 256
           ++     D +TY+++I+ Y   G  +    VL E++A  ++P+ + ++ L+  +   G  
Sbjct: 368 EKRGVSPDEHTYSLLIDAYVNAGRWESARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEW 427

Query: 257 EEAAQLVKEMREKRIEPDRITY 276
           ++  Q++KEM+   ++PDR  Y
Sbjct: 428 QKTFQVLKEMKSIGVKPDRQFY 447


HSP 4 Score: 83.6 bits (205), Expect = 2.3e-16
Identity = 63/273 (23.08%), Postives = 118/273 (43.22%), Query Frame = 1

Query: 21  QQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVD 80
           Q  I P       +L+ Y + G +     +   + K GVS D+  Y+ +I+    A   +
Sbjct: 332 QSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEMEKRGVSPDEHTYSLLIDAYVNAGRWE 391

Query: 81  ELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLV-DAISYNTMI 140
               +  EM      PN+   + +L  +     + K   +    +  G+  D   YN +I
Sbjct: 392 SARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEWQKTFQVLKEMKSIGVKPDRQFYNVVI 451

Query: 141 SVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSE 200
             +GK     +  +T  +M   G       +N ++D + K  +      + + M+     
Sbjct: 452 DTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCHCKHGRHIVAEEMFEAMERRGCL 511

Query: 201 CDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQL 260
               TYNIMIN YG+Q   D++  +L ++K+ G+ P++ ++ TL+  YG +G   +A + 
Sbjct: 512 PCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNVVTHTTLVDVYGKSGRFNDAIEC 571

Query: 261 VKEMREKRIEPDRITYINMIRALQRNDQFLEAV 293
           ++EM+   ++P    Y  +I A  +     +AV
Sbjct: 572 LEEMKSVGLKPSSTMYNALINAYAQRGLSEQAV 604


HSP 5 Score: 60.1 bits (144), Expect = 2.8e-09
Identity = 45/240 (18.75%), Postives = 105/240 (43.75%), Query Frame = 1

Query: 66  YNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKAR--NLFGL 125
           YN +I  C+R   +++   L  +M Q G+  + V  ++++    +S          L+  
Sbjct: 200 YNALIGACARNNDIEKALNLIAKMRQDGYQSDFVNYSLVIQSLTRSNKIDSVMLLRLYKE 259

Query: 126 AQKRGL-VDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQ 185
            ++  L +D    N +I  + K+ D       +   +  G S        ++ A     +
Sbjct: 260 IERDKLELDVQLVNDIIMGFAKSGDPSKALQLLGMAQATGLSAKTATLVSIISALADSGR 319

Query: 186 MENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNT 245
                ++ + ++++  +     YN ++  Y + G + +   +++E++  G+ PD ++Y+ 
Sbjct: 320 TLEAEALFEELRQSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEMEKRGVSPDEHTYSL 379

Query: 246 LIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQMK 303
           LI AY  AG  E A  ++KEM    ++P+   +  ++   +   ++ +  +    +K+MK
Sbjct: 380 LIDAYVNAGRWESARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEWQKTFQ---VLKEMK 436

BLAST of Csa4G098200 vs. TAIR10
Match: AT5G39980.1 (AT5G39980.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 127.1 bits (318), Expect = 1.8e-29
Identity = 81/282 (28.72%), Postives = 142/282 (50.35%), Query Frame = 1

Query: 4   KAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQ 63
           K G  + A S L  M EQ  +  D+ L  +++ + +R     K   ++ R+ +SG++ D 
Sbjct: 202 KEGMFDSALSWLQKM-EQDRVSGDLVLYSNLIELSRRLCDYSKAISIFSRLKRSGITPDL 261

Query: 64  EMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFG- 123
             YN +IN   +A    E   L  EM + G  PNTV+ + +L VY ++  F +A ++F  
Sbjct: 262 VAYNSMINVYGKAKLFREARLLIKEMNEAGVLPNTVSYSTLLSVYVENHKFLEALSVFAE 321

Query: 124 LAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQ 183
           + +    +D  + N MI VYG+    K        ++      ++ +YN +L  YG+   
Sbjct: 322 MKEVNCALDLTTCNIMIDVYGQLDMVKEADRLFWSLRKMDIEPNVVSYNTILRVYGEAEL 381

Query: 184 MENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNT 243
                 + + MQ    E +  TYN MI IYG+    ++   ++ E+++ G+EP+  +Y+T
Sbjct: 382 FGEAIHLFRLMQRKDIEQNVVTYNTMIKIYGKTMEHEKATNLVQEMQSRGIEPNAITYST 441

Query: 244 LIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQR 285
           +I  +G AG ++ AA L +++R   +E D++ Y  MI A +R
Sbjct: 442 IISIWGKAGKLDRAATLFQKLRSSGVEIDQVLYQTMIVAYER 482


HSP 2 Score: 126.7 bits (317), Expect = 2.4e-29
Identity = 78/278 (28.06%), Postives = 140/278 (50.36%), Query Frame = 1

Query: 3   VKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWD 62
           ++A   + A  + D M  Q+ + PD Y    ++  + + GM         ++ +  VS D
Sbjct: 166 LRAKQFDIAHGLFDEM-RQRALAPDRYTYSTLITSFGKEGMFDSALSWLQKMEQDRVSGD 225

Query: 63  QEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFG 122
             +Y+ +I    R     +   +F  + + G  P+ V  N M++VYGK+KLF +AR L  
Sbjct: 226 LVLYSNLIELSRRLCDYSKAISIFSRLKRSGITPDLVAYNSMINVYGKAKLFREARLLIK 285

Query: 123 LAQKRGLV-DAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKEC 182
              + G++ + +SY+T++SVY +N  F    S   +MK    ++ L   N M+D YG+  
Sbjct: 286 EMNEAGVLPNTVSYSTLLSVYVENHKFLEALSVFAEMKEVNCALDLTTCNIMIDVYGQLD 345

Query: 183 QMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYN 242
            ++    +   +++   E +  +YN ++ +YGE     E   +   ++   +E ++ +YN
Sbjct: 346 MVKEADRLFWSLRKMDIEPNVVSYNTILRVYGEAELFGEAIHLFRLMQRKDIEQNVVTYN 405

Query: 243 TLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMI 280
           T+IK YG     E+A  LV+EM+ + IEP+ ITY  +I
Sbjct: 406 TMIKIYGKTMEHEKATNLVQEMQSRGIEPNAITYSTII 442


HSP 3 Score: 110.9 bits (276), Expect = 1.4e-24
Identity = 73/273 (26.74%), Postives = 133/273 (48.72%), Query Frame = 1

Query: 20  EQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPV 79
           ++  I PD+     M+ +Y +  +  +   L   + ++GV  +   Y+ +++        
Sbjct: 252 KRSGITPDLVAYNSMINVYGKAKLFREARLLIKEMNEAGVLPNTVSYSTLLSVYVENHKF 311

Query: 80  DELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLV-DAISYNTM 139
            E   +F EM +   A +  T N+M+DVYG+  +  +A  LF   +K  +  + +SYNT+
Sbjct: 312 LEALSVFAEMKEVNCALDLTTCNIMIDVYGQLDMVKEADRLFWSLRKMDIEPNVVSYNTI 371

Query: 140 ISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSS 199
           + VYG+ + F       + M+      ++  YN M+  YGK  + E   +++Q MQ    
Sbjct: 372 LRVYGEAELFGEAIHLFRLMQRKDIEQNVVTYNTMIKIYGKTMEHEKATNLVQEMQSRGI 431

Query: 200 ECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQ 259
           E +  TY+ +I+I+G+ G +D  A +  +L++ G+E D   Y T+I AY   G++  A +
Sbjct: 432 EPNAITYSTIISIWGKAGKLDRAATLFQKLRSSGVEIDQVLYQTMIVAYERVGLMGHAKR 491

Query: 260 LVKEMREKRIEPDRITYINMIRALQRNDQFLEA 292
           L+ E++     PD I     I  L +  +  EA
Sbjct: 492 LLHELK----LPDNIPRETAITILAKAGRTEEA 520


HSP 4 Score: 107.5 bits (267), Expect = 1.5e-23
Identity = 74/294 (25.17%), Postives = 138/294 (46.94%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           +Y KA    +A  ++  M E   ++P+      +L +Y       +   ++  + +   +
Sbjct: 269 VYGKAKLFREARLLIKEMNEA-GVLPNTVSYSTLLSVYVENHKFLEALSVFAEMKEVNCA 328

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
            D    N +I+   +   V E  RLF  + +    PN V+ N +L VYG+++LF +A +L
Sbjct: 329 LDLTTCNIMIDVYGQLDMVKEADRLFWSLRKMDIEPNVVSYNTILRVYGEAELFGEAIHL 388

Query: 121 FGLAQKRGL-VDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 180
           F L Q++ +  + ++YNTMI +YGK  + +  ++ VQ+M+  G   +   Y+ ++  +GK
Sbjct: 389 FRLMQRKDIEQNVVTYNTMIKIYGKTMEHEKATNLVQEMQSRGIEPNAITYSTIISIWGK 448

Query: 181 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 240
             +++   ++ Q+++ +  E D   Y  MI  Y   G +     +L ELK     PD   
Sbjct: 449 AGKLDRAATLFQKLRSSGVEIDQVLYQTMIVAYERVGLMGHAKRLLHELKL----PDNIP 508

Query: 241 YNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVK 294
             T I     AG  EEA  + ++  E     D   +  MI    RN +++  ++
Sbjct: 509 RETAITILAKAGRTEEATWVFRQAFESGEVKDISVFGCMINLYSRNQRYVNVIE 557


HSP 5 Score: 79.7 bits (195), Expect = 3.4e-15
Identity = 44/168 (26.19%), Postives = 82/168 (48.81%), Query Frame = 1

Query: 134 SYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRM 193
           +YN ++    + K F        +M+    +     Y+ ++ ++GKE   ++  S LQ+M
Sbjct: 157 AYNVVLRNVLRAKQFDIAHGLFDEMRQRALAPDRYTYSTLITSFGKEGMFDSALSWLQKM 216

Query: 194 QETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMV 253
           ++     D   Y+ +I +        +   + + LK  G+ PDL +YN++I  YG A + 
Sbjct: 217 EQDRVSGDLVLYSNLIELSRRLCDYSKAISIFSRLKRSGITPDLVAYNSMINVYGKAKLF 276

Query: 254 EEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQM 302
            EA  L+KEM E  + P+ ++Y  ++     N +FLEA+     MK++
Sbjct: 277 REARLLIKEMNEAGVLPNTVSYSTLLSVYVENHKFLEALSVFAEMKEV 324


HSP 6 Score: 75.5 bits (184), Expect = 6.4e-14
Identity = 62/272 (22.79%), Postives = 125/272 (45.96%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           +Y K    E A +++  M + + I P+      ++ I+ + G + + A L+ ++  SGV 
Sbjct: 409 IYGKTMEHEKATNLVQEM-QSRGIEPNAITYSTIISIWGKAGKLDRAATLFQKLRSSGVE 468

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
            DQ +Y  +I    R   +    RL  E+      P+ +     + +  K+    +A  +
Sbjct: 469 IDQVLYQTMIVAYERVGLMGHAKRLLHELK----LPDNIPRETAITILAKAGRTEEATWV 528

Query: 121 FGLAQKRGLVDAIS-YNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 180
           F  A + G V  IS +  MI++Y +N+ + N+    +KM+  G+         +L+AYGK
Sbjct: 529 FRQAFESGEVKDISVFGCMINLYSRNQRYVNVIEVFEKMRTAGYFPDSNVIAMVLNAYGK 588

Query: 181 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 240
           + + E   +V + MQE         +  M+++Y  +   + V  +   L++   +P++ S
Sbjct: 589 QREFEKADTVYREMQEEGCVFPDEVHFQMLSLYSSKKDFEMVESLFQRLES---DPNVNS 648

Query: 241 ---YNTLIKAYGIAGMVEEAAQLVKEMREKRI 269
              +  +   Y  A  + +A++++  MRE+ I
Sbjct: 649 KELHLVVAALYERADKLNDASRVMNRMRERGI 672

BLAST of Csa4G098200 vs. TAIR10
Match: AT3G18110.1 (AT3G18110.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 122.1 bits (305), Expect = 5.9e-28
Identity = 82/301 (27.24%), Postives = 148/301 (49.17%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCG-MVHKLA-DLYYRILKSG 60
           +Y ++G    A  ++D M  Q+  VPD+     ++    + G +   LA +L   +  SG
Sbjct: 234 VYSRSGKFSKAQELVDAM-RQRGCVPDLISFNTLINARLKSGGLTPNLAVELLDMVRNSG 293

Query: 61  VSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKAR 120
           +  D   YN +++ CSR   +D   ++F++M      P+  T N M+ VYG+  L  +A 
Sbjct: 294 LRPDAITYNTLLSACSRDSNLDGAVKVFEDMEAHRCQPDLWTYNAMISVYGRCGLAAEAE 353

Query: 121 NLFGLAQKRGLV-DAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAY 180
            LF   + +G   DA++YN+++  + + ++ + +    Q+M+  GF      YN ++  Y
Sbjct: 354 RLFMELELKGFFPDAVTYNSLLYAFARERNTEKVKEVYQQMQKMGFGKDEMTYNTIIHMY 413

Query: 181 GKECQMENFRSVLQRMQETSS-ECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPD 240
           GK+ Q++    + + M+  S    D  TY ++I+  G+     E A +++E+   G++P 
Sbjct: 414 GKQGQLDLALQLYKDMKGLSGRNPDAITYTVLIDSLGKANRTVEAAALMSEMLDVGIKPT 473

Query: 241 LYSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSL 298
           L +Y+ LI  Y  AG  EEA      M     +PD + Y  M+  L R ++  +A  W L
Sbjct: 474 LQTYSALICGYAKAGKREEAEDTFSCMLRSGTKPDNLAYSVMLDVLLRGNETRKA--WGL 531


HSP 2 Score: 102.8 bits (255), Expect = 3.7e-22
Identity = 70/280 (25.00%), Postives = 131/280 (46.79%), Query Frame = 1

Query: 21   QQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVD 80
            Q    PD+     ++  Y +CG   +   ++  +++ G S   E  N +++       ++
Sbjct: 780  QSGRTPDLKTWNSLMSAYAQCGCYERARAIFNTMMRDGPSPTVESINILLHALCVDGRLE 839

Query: 81   ELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLVDAIS-YNTMI 140
            EL  + +E+   GF  +  ++ +MLD + ++    + + ++   +  G +  I  Y  MI
Sbjct: 840  ELYVVVEELQDMGFKISKSSILLMLDAFARAGNIFEVKKIYSSMKAAGYLPTIRLYRMMI 899

Query: 141  SVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSE 200
             +  K K  ++    V +M+   F V L  +N ML  Y      +    V QR++ET  E
Sbjct: 900  ELLCKGKRVRDAEIMVSEMEEANFKVELAIWNSMLKMYTAIEDYKKTVQVYQRIKETGLE 959

Query: 201  CDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQL 260
             D  TYN +I +Y      +E   ++ +++  GL+P L +Y +LI A+G    +E+A QL
Sbjct: 960  PDETTYNTLIIMYCRDRRPEEGYLLMQQMRNLGLDPKLDTYKSLISAFGKQKCLEQAEQL 1019

Query: 261  VKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMK 300
             +E+  K ++ DR  Y  M++  + +    +A K    MK
Sbjct: 1020 FEELLSKGLKLDRSFYHTMMKISRDSGSDSKAEKLLQMMK 1059

BLAST of Csa4G098200 vs. NCBI nr
Match: gi|449457967|ref|XP_004146719.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g30825, chloroplastic [Cucumis sativus])

HSP 1 Score: 615.5 bits (1586), Expect = 4.9e-173
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS
Sbjct: 592 MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 651

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL
Sbjct: 652 WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 711

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE
Sbjct: 712 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 771

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
           CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY
Sbjct: 772 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 831

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ
Sbjct: 832 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 891

Query: 301 MKY 304
           MKY
Sbjct: 892 MKY 894

BLAST of Csa4G098200 vs. NCBI nr
Match: gi|449457967|ref|XP_004146719.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g30825, chloroplastic [Cucumis sativus])

HSP 1 Score: 95.9 bits (237), Expect = 1.3e-16
Identity = 56/229 (24.45%), Postives = 112/229 (48.91%), Query Frame = 1

Query: 58  GVSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKA 117
           G   D +++N +I  C ++  V++ ++ F  ML+C   PN  T  +++ +Y K     ++
Sbjct: 195 GSQLDFQVFNTLIYACYKSRFVEQGTKWFRMMLECQVQPNVATFGMLMGLYQKKCDIKES 254

Query: 118 RNLFGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAY 177
              F   +  G+V   +Y +MI++Y +   +      +Q M+ +    +LE +  ML+AY
Sbjct: 255 EFAFNQMRNFGIVCETAYASMITIYIRMNLYDKAEEVIQLMQEDKVIPNLENWVVMLNAY 314

Query: 178 GKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDL 237
            ++ +ME    V   M+E     +   YN +I  YG+   +D    +   +K  G+EPD 
Sbjct: 315 CQQGKMEEAELVFASMEEAGFSSNIIAYNTLITGYGKASNMDTAQRLFLGIKNSGVEPDE 374

Query: 238 YSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRND 287
            +Y ++I+ +G AG  + A    KE++ +   P+      +I    +++
Sbjct: 375 TTYRSMIEGWGRAGNYKMAEWYYKELKRRGYMPNSSNLFTLINLQAKHE 423


HSP 2 Score: 67.4 bits (163), Expect = 4.9e-08
Identity = 64/286 (22.38%), Postives = 132/286 (46.15%), Query Frame = 1

Query: 2   YVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKS-GVS 61
           Y KA ++ D    L L  +   + PD    R M+  + R G  +K+A+ YY+ LK  G  
Sbjct: 349 YGKASNM-DTAQRLFLGIKNSGVEPDETTYRSMIEGWGRAGN-YKMAEWYYKELKRRGYM 408

Query: 62  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 121
            +      +IN  ++        +  ++ML+ G  P+++  NV L  Y K++       L
Sbjct: 409 PNSSNLFTLINLQAKHEDEAGTLKTLNDMLKIGCRPSSIVGNV-LQAYEKARRMKSVPVL 468

Query: 122 F-GLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 181
             G   ++ L    S + ++  Y K+    +    +++ ++         Y+ ++ +  +
Sbjct: 469 LTGSFYRKVLSSQTSCSILVMAYVKHCLVDDALKVLREKEWKDHHFEENLYHLLICSCKE 528

Query: 182 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 241
              +EN   +  ++ +  ++ + +    MI+IY   G   +  ++   L++ G+  DL +
Sbjct: 529 LGHLENAIKIYTQLPKRENKPNLHITCTMIDIYSIMGRFSDGEKLYLSLRSSGIPLDLIA 588

Query: 242 YNTLIKAYGIAGMVEEAAQLVKEMREKR-IEPDRITYINMIRALQR 285
           YN +++ Y  AG +E+A  ++  M E++ I PD     +M+R  QR
Sbjct: 589 YNVVVRMYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQR 631


HSP 3 Score: 57.0 bits (136), Expect = 6.7e-05
Identity = 62/300 (20.67%), Postives = 135/300 (45.00%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           +Y++    + A  V+ LM E + ++P++     ML  Y + G + +   ++  + ++G S
Sbjct: 278 IYIRMNLYDKAEEVIQLMQEDK-VIPNLENWVVMLNAYCQQGKMEEAELVFASMEEAGFS 337

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
            +   YN +I    +A  +D   RLF  +   G  P+  T   M++ +G++  +  A   
Sbjct: 338 SNIIAYNTLITGYGKASNMDTAQRLFLGIKNSGVEPDETTYRSMIEGWGRAGNYKMAEWY 397

Query: 121 FGLAQKRG-LVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 180
           +   ++RG + ++ +  T+I++  K++D      T+  M   G   S    N +L AY K
Sbjct: 398 YKELKRRGYMPNSSNLFTLINLQAKHEDEAGTLKTLNDMLKIGCRPSSIVGN-VLQAYEK 457

Query: 181 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 240
             +M++   +L             + +I++  Y +   +D+  +VL E +      +   
Sbjct: 458 ARRMKSVPVLLTGSFYRKVLSSQTSCSILVMAYVKHCLVDDALKVLREKEWKDHHFEENL 517

Query: 241 YNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMK 300
           Y+ LI +    G +E A ++  ++ ++  +P+      MI       +F +  K  L ++
Sbjct: 518 YHLLICSCKELGHLENAIKIYTQLPKRENKPNLHITCTMIDIYSIMGRFSDGEKLYLSLR 575


HSP 4 Score: 615.5 bits (1586), Expect = 4.9e-173
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS
Sbjct: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL
Sbjct: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE
Sbjct: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
           CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY
Sbjct: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ
Sbjct: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300

Query: 301 MKY 304
           MKY
Sbjct: 301 MKY 303

BLAST of Csa4G098200 vs. NCBI nr
Match: gi|659086251|ref|XP_008443835.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g30825, chloroplastic [Cucumis melo])

HSP 1 Score: 600.1 bits (1546), Expect = 2.1e-168
Identity = 292/303 (96.37%), Postives = 300/303 (99.01%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAGSLEDACSVLDLMAEQQDIVPD+YLLRDMLRIYQRCGMVHKL+DLYYRILKSGVS
Sbjct: 596 MYVKAGSLEDACSVLDLMAEQQDIVPDVYLLRDMLRIYQRCGMVHKLSDLYYRILKSGVS 655

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLF KARNL
Sbjct: 656 WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFAKARNL 715

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           FG AQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQ+MKFNGFSVSLEAYNCMLDAYGKE
Sbjct: 716 FGFAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQQMKFNGFSVSLEAYNCMLDAYGKE 775

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
           CQMENFRSVLQRMQE++SECDHYTYNIMINIYGE+GWIDEVAEVLTELKACGLEPDLYSY
Sbjct: 776 CQMENFRSVLQRMQESTSECDHYTYNIMINIYGERGWIDEVAEVLTELKACGLEPDLYSY 835

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGIAGMVEEAA+LVKEMREK IEPDRITYINMIRALQRNDQFLEA+KWSLWMKQ
Sbjct: 836 NTLIKAYGIAGMVEEAARLVKEMREKGIEPDRITYINMIRALQRNDQFLEAIKWSLWMKQ 895

Query: 301 MKY 304
           MKY
Sbjct: 896 MKY 898

BLAST of Csa4G098200 vs. NCBI nr
Match: gi|659086251|ref|XP_008443835.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g30825, chloroplastic [Cucumis melo])

HSP 1 Score: 97.4 bits (241), Expect = 4.4e-17
Identity = 60/236 (25.42%), Postives = 115/236 (48.73%), Query Frame = 1

Query: 58  GVSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKA 117
           G   D +++N +I  C ++  V+  ++ F  ML+C   PN  T  +++ +Y KS    ++
Sbjct: 199 GSQLDFQVFNTLIYACYKSGFVEWGTKWFRMMLECQVQPNVATFGMLMGLYQKSCDIEES 258

Query: 118 RNLFGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAY 177
              F   +  G+V   +Y +MI++Y +   +      +Q M+ +    +LE +  ML+AY
Sbjct: 259 EFAFNQMRNFGIVCETAYASMITIYIRMNLYDKAEEVIQLMQKDKVIPNLENWLVMLNAY 318

Query: 178 GKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDL 237
            ++ +ME    V   M+E     +   YN +I  YG+   +D    +   +K  G+EPD 
Sbjct: 319 CQQGKMEEAELVFASMEEAGFSSNIIAYNTLITGYGKASNMDTAQRLFLGIKNSGVEPDE 378

Query: 238 YSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVK 294
            +Y ++I+ +G AG  + A    KE++ K   P+      +I    +++    A+K
Sbjct: 379 TTYRSMIEGWGRAGNYKMAEWYYKELKRKGYMPNSSNLFTLINLQAKHEDEAGALK 434


HSP 2 Score: 63.9 bits (154), Expect = 5.5e-07
Identity = 63/286 (22.03%), Postives = 130/286 (45.45%), Query Frame = 1

Query: 2   YVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILK-SGVS 61
           Y KA ++ D    L L  +   + PD    R M+  + R G  +K+A+ YY+ LK  G  
Sbjct: 353 YGKASNM-DTAQRLFLGIKNSGVEPDETTYRSMIEGWGRAGN-YKMAEWYYKELKRKGYM 412

Query: 62  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 121
            +      +IN  ++        +  ++ML+ G  P+++  NV L  Y K++       L
Sbjct: 413 PNSSNLFTLINLQAKHEDEAGALKTLNDMLKIGCRPSSIVGNV-LQAYEKARRIKSVPVL 472

Query: 122 F-GLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGK 181
             G   ++ L    S + ++  Y K+    +    +++ ++         Y+ ++ +  +
Sbjct: 473 LTGSFYRKVLSSQTSCSILVMAYVKHCLVDDALKVLREKEWKDHHFEENLYHLLICSCKE 532

Query: 182 ECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYS 241
               E+   +  +  +  ++ + +    MI+IY   G   +  ++   L++ G+  DL +
Sbjct: 533 LGHFESAIKIYAQRPKRENKPNLHITCTMIDIYSIMGRFSDGEKLYLSLRSSGIPLDLIA 592

Query: 242 YNTLIKAYGIAGMVEEAAQLVKEMREKR-IEPDRITYINMIRALQR 285
           YN +++ Y  AG +E+A  ++  M E++ I PD     +M+R  QR
Sbjct: 593 YNVVVRMYVKAGSLEDACSVLDLMAEQQDIVPDVYLLRDMLRIYQR 635


HSP 3 Score: 494.6 bits (1272), Expect = 1.3e-136
Identity = 237/301 (78.74%), Postives = 266/301 (88.37%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAG+LEDACSVLD M +Q+ IVPDIY+ RDMLRIYQRCG + KL DLYY++LKSGV+
Sbjct: 464 MYVKAGALEDACSVLDTMDKQEGIVPDIYMFRDMLRIYQRCGRLDKLKDLYYKLLKSGVT 523

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           WDQEMYNCVINCCSRALPVDE+S +FDEMLQCGF PNT+T NVMLDVYGK+KL  KAR L
Sbjct: 524 WDQEMYNCVINCCSRALPVDEISEIFDEMLQCGFVPNTITFNVMLDVYGKAKLLKKARKL 583

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           F +AQK GLVD ISYNT+I+ YG+NKD +NMSST  +M+F GFSVSLEAYN MLDAYGKE
Sbjct: 584 FWMAQKWGLVDMISYNTIIAAYGRNKDLRNMSSTFGEMQFKGFSVSLEAYNTMLDAYGKE 643

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
            QME FRSVLQRM+ETS   DHYTYNIMINIYGEQGWIDEVA+VLTELK CGL PDL SY
Sbjct: 644 SQMERFRSVLQRMKETSCASDHYTYNIMINIYGEQGWIDEVADVLTELKECGLGPDLCSY 703

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGIAGMVE+A  LVKEMRE  I+PD+ITYIN+I AL++ND++LEAVKWSLWMKQ
Sbjct: 704 NTLIKAYGIAGMVEDAVHLVKEMRENGIQPDKITYINLINALRKNDEYLEAVKWSLWMKQ 763

Query: 301 M 302
           M
Sbjct: 764 M 764

BLAST of Csa4G098200 vs. NCBI nr
Match: gi|595814264|ref|XP_007203708.1| (hypothetical protein PRUPE_ppa019391mg, partial [Prunus persica])

HSP 1 Score: 83.6 bits (205), Expect = 6.6e-13
Identity = 53/236 (22.46%), Postives = 114/236 (48.31%), Query Frame = 1

Query: 58  GVSWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKA 117
           G   + +++N +I  C +   ++   + F  ML+    PN  T  +++ +Y K     +A
Sbjct: 67  GCELNYQVFNTLIYACCKLGRLELGGKWFRMMLEHEVQPNIATFGMLMVLYQKGWNVEEA 126

Query: 118 RNLFGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAY 177
              F   +  G++   +Y++MI++Y +   F+     +  +K +   ++L+ +  M++AY
Sbjct: 127 EFTFFQMRNFGILCQSAYSSMITIYTRLNLFEKAEEIIGLLKEDRVRLNLDNWLVMINAY 186

Query: 178 GKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDL 237
            ++ ++++   VL  MQE     +   YN +I  YG+   +D    +   +K  GLEPD 
Sbjct: 187 CQQGKVDDAELVLVSMQEAGFSPNIIAYNTLITGYGKASKMDAADHLFQGIKNAGLEPDE 246

Query: 238 YSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVK 294
            +Y ++I+ +G A    EA    KE++    +P+      +I    +++    A++
Sbjct: 247 TTYRSMIEGWGRADNYMEAEWYYKELKRLGYKPNSSNLYTLINLQAKHEDEEGAIR 302


HSP 2 Score: 82.0 bits (201), Expect = 1.9e-12
Identity = 63/273 (23.08%), Postives = 120/273 (43.96%), Query Frame = 1

Query: 33  DMLRIYQRCGMVHKLADLYYRILKSGVSWDQEMYNCVINCCSRALPVDELSRLFDEML-Q 92
           D+L+  +RC  V  L    +      +  +   +N V+    R    D   +L  E++  
Sbjct: 6   DILKRLERCSDVKTLRFFEWMRSNGKLERNVSAFNLVLRVMGRREDWDGAEKLVQEVIAD 65

Query: 93  CGFAPNTVTLNVMLDVYGKSKLFTKARNLFGLAQKRGLVDAIS-YNTMISVYGKNKDFKN 152
            G   N    N ++    K          F +  +  +   I+ +  ++ +Y K  + + 
Sbjct: 66  LGCELNYQVFNTLIYACCKLGRLELGGKWFRMMLEHEVQPNIATFGMLMVLYQKGWNVEE 125

Query: 153 MSSTVQKMKFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSECDHYTYNIMIN 212
              T  +M+  G  +   AY+ M+  Y +    E    ++  ++E     +   + +MIN
Sbjct: 126 AEFTFFQMRNFGI-LCQSAYSSMITIYTRLNLFEKAEEIIGLLKEDRVRLNLDNWLVMIN 185

Query: 213 IYGEQGWIDEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEP 272
            Y +QG +D+   VL  ++  G  P++ +YNTLI  YG A  ++ A  L + ++   +EP
Sbjct: 186 AYCQQGKVDDAELVLVSMQEAGFSPNIIAYNTLITGYGKASKMDAADHLFQGIKNAGLEP 245

Query: 273 DRITYINMIRALQRNDQFLEAVKWSLWMKQMKY 304
           D  TY +MI    R D ++EA  +   +K++ Y
Sbjct: 246 DETTYRSMIEGWGRADNYMEAEWYYKELKRLGY 277


HSP 3 Score: 82.0 bits (201), Expect = 1.9e-12
Identity = 57/247 (23.08%), Postives = 120/247 (48.58%), Query Frame = 1

Query: 45  HKLADLYYRILKSGV----SWDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVT 104
           H L D   ++L+  +     ++  +Y+ +I  C     ++   +++ +M +    PN   
Sbjct: 363 HCLVDDTMKVLREKLWKDPPFEDNLYHLLICSCKELGHLENAVKIYKQMPRYDDKPNMHI 422

Query: 105 LNVMLDVYGKSKLFTKARNLFGLAQKRGL-VDAISYNTMISVYGKNKDFKNMSSTVQKM- 164
           +  M+D+Y    LFT+A  ++   +  G+ +D I+Y+  + +Y K    ++  S +  M 
Sbjct: 423 MCTMIDIYIIMGLFTEAEKIYVELKSSGVALDMIAYSIAVRMYVKAGALEDACSVLDTMD 482

Query: 165 KFNGFSVSLEAYNCMLDAYGKECQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWI 224
           K  G    +  +  ML  Y +  +++  + +  ++ ++    D   YN +IN       +
Sbjct: 483 KQEGIVPDIYMFRDMLRIYQRCGRLDKLKDLYYKLLKSGVTWDQEMYNCVINCCSRALPV 542

Query: 225 DEVAEVLTELKACGLEPDLYSYNTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINM 284
           DE++E+  E+  CG  P+  ++N ++  YG A ++++A +L   M +K    D I+Y  +
Sbjct: 543 DEISEIFDEMLQCGFVPNTITFNVMLDVYGKAKLLKKARKLF-WMAQKWGLVDMISYNTI 602

Query: 285 IRALQRN 286
           I A  RN
Sbjct: 603 IAAYGRN 608


HSP 4 Score: 493.8 bits (1270), Expect = 2.1e-136
Identity = 235/301 (78.07%), Postives = 266/301 (88.37%), Query Frame = 1

Query: 1   MYVKAGSLEDACSVLDLMAEQQDIVPDIYLLRDMLRIYQRCGMVHKLADLYYRILKSGVS 60
           MYVKAGSLEDACSVL+ M EQ+ IVPDIY+ RDMLRIYQRCG + KL DLYY++LKSGV+
Sbjct: 599 MYVKAGSLEDACSVLEAMEEQEGIVPDIYMFRDMLRIYQRCGRLDKLKDLYYKLLKSGVT 658

Query: 61  WDQEMYNCVINCCSRALPVDELSRLFDEMLQCGFAPNTVTLNVMLDVYGKSKLFTKARNL 120
           WD+EMYNCVINCCS ALPVDE+S +FDEMLQCGF PNT+T NVMLDVYGK++L  KAR L
Sbjct: 659 WDREMYNCVINCCSHALPVDEISEIFDEMLQCGFVPNTITFNVMLDVYGKARLLKKAREL 718

Query: 121 FGLAQKRGLVDAISYNTMISVYGKNKDFKNMSSTVQKMKFNGFSVSLEAYNCMLDAYGKE 180
           F +AQK GLVD ISYNT+I+ YG+NKDF++MSST Q+M+F GFSVSLEAYN MLDAYGKE
Sbjct: 719 FRMAQKWGLVDMISYNTIIAAYGRNKDFRSMSSTFQEMQFKGFSVSLEAYNSMLDAYGKE 778

Query: 181 CQMENFRSVLQRMQETSSECDHYTYNIMINIYGEQGWIDEVAEVLTELKACGLEPDLYSY 240
            QME FRS+LQRM++TS   DHYTYNIMINIYGEQGWIDEVA VLTELK CGL PDL SY
Sbjct: 779 SQMERFRSILQRMKKTSCASDHYTYNIMINIYGEQGWIDEVAGVLTELKECGLGPDLCSY 838

Query: 241 NTLIKAYGIAGMVEEAAQLVKEMREKRIEPDRITYINMIRALQRNDQFLEAVKWSLWMKQ 300
           NTLIKAYGIAGMVE+A  LVKEMRE  +EPD+ITY+N+I ALQRND++LEAVKWSLWMKQ
Sbjct: 839 NTLIKAYGIAGMVEDAVHLVKEMRENGVEPDKITYVNLIAALQRNDEYLEAVKWSLWMKQ 898

Query: 301 M 302
           M
Sbjct: 899 M 899

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP342_ARATH1.2e-12671.43Pentatricopeptide repeat-containing protein At4g30825, chloroplastic OS=Arabidop... [more]
PP362_ARATH1.4e-3127.30Pentatricopeptide repeat-containing protein At5g02860 OS=Arabidopsis thaliana GN... [more]
PP413_ARATH1.2e-3029.17Pentatricopeptide repeat-containing protein At5g42310, mitochondrial OS=Arabidop... [more]
PP408_ARATH3.3e-2828.72Pentatricopeptide repeat-containing protein At5g39980, chloroplastic OS=Arabidop... [more]
PP241_ARATH1.1e-2627.24Pentatricopeptide repeat-containing protein At3g18110, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A0A0LTR9_CUCSA3.4e-173100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G257890 PE=4 SV=1[more]
A0A0A0LTR9_CUCSA9.0e-1724.45Uncharacterized protein OS=Cucumis sativus GN=Csa_1G257890 PE=4 SV=1[more]
M5VU43_PRUPE4.6e-1322.46Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa019391mg PE=4 S... [more]
D7U7F9_VITVI3.2e-1424.07Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0013g02120 PE=4 SV=... [more]
A0A0D2MWJ6_GOSRA6.9e-1727.65Uncharacterized protein OS=Gossypium raimondii GN=B456_004G107200 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G30825.16.6e-12871.43 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT5G02860.18.0e-3327.30 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT5G42310.16.8e-3229.17 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT5G39980.11.8e-2928.72 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT3G18110.15.9e-2827.24 Pentatricopeptide repeat (PPR) superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449457967|ref|XP_004146719.1|4.9e-173100.00PREDICTED: pentatricopeptide repeat-containing protein At4g30825, chloroplastic ... [more]
gi|449457967|ref|XP_004146719.1|1.3e-1624.45PREDICTED: pentatricopeptide repeat-containing protein At4g30825, chloroplastic ... [more]
gi|659086251|ref|XP_008443835.1|2.1e-16896.37PREDICTED: pentatricopeptide repeat-containing protein At4g30825, chloroplastic ... [more]
gi|659086251|ref|XP_008443835.1|4.4e-1725.42PREDICTED: pentatricopeptide repeat-containing protein At4g30825, chloroplastic ... [more]
gi|595814264|ref|XP_007203708.1|6.6e-1322.46hypothetical protein PRUPE_ppa019391mg, partial [Prunus persica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
IPR004575MAT1/Tfb3
IPR011990TPR-like_helical_dom_sf
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: Biological Process
TermDefinition
GO:0007049cell cycle
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007049 cell cycle
biological_process GO:0016556 mRNA modification
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa4G098200.1Csa4G098200.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 133..163
score: 6.0E-5coord: 2..20
score:
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 235..282
score: 3.3E-13coord: 65..108
score: 1.4E-8coord: 169..210
score: 7.
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 169..201
score: 1.0E-5coord: 203..236
score: 1.0E-7coord: 65..98
score: 6.1E-7coord: 133..166
score: 2.7E-6coord: 99..130
score: 3.5E-4coord: 238..271
score: 1.6E-10coord: 2..28
score: 4.
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 201..235
score: 11.575coord: 1..21
score: 5.722coord: 236..270
score: 13.197coord: 97..127
score: 7.541coord: 62..96
score: 10.819coord: 271..303
score: 7.333coord: 27..61
score: 6.96coord: 131..165
score: 9.734coord: 166..200
score: 8
IPR004575Cdk-activating kinase assembly factor MAT1/Tfb3PANTHERPTHR12683FAMILY NOT NAMEDcoord: 1..303
score: 6.0E
IPR011990Tetratricopeptide-like helical domainGENE3DG3DSA:1.25.40.10coord: 64..294
score: 2.
NoneNo IPR availableunknownCoilCoilcoord: 249..269
scor
NoneNo IPR availablePANTHERPTHR12683:SF10SUBFAMILY NOT NAMEDcoord: 1..303
score: 6.0E

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None