Csa3G890080 (gene) Cucumber (Chinese Long) v2

NameCsa3G890080
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionPectinesterase; contains IPR006501 (Pectinesterase inhibitor), IPR011050 (Pectin lyase fold/virulence factor)
LocationChr3 : 38011175 .. 38014522 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATCTCAGGTATACTTTCAGGCTAATCTCTTTCCATTACAACAATAACAATGAAAAGTTTCGTCCAAAAAACCTCTTGCGAATTTCTTTGAGCACACGGTTTAGCTTGAAACTCACATGGTAAGTCCATCATTGGTTTTGCATTGTGATGTTCAAGAAAAGCAAATAAATCTTGCATTTATTTTAGTCTTGTCTCTTAGCACAACATTTGAGGAAGCATGGATTACTCAATCTAGCGTAAGTTGAAATATAATATGCATTTGTGAATGTTTTATGCTACACTTAATCGAGAAAGTGGCTAGACTATTGACCAACTGTTTTGTGTTCTTGAAGTTATATTGTTGACTTTTCCTTAAAATAATGTCAATTTGCAGGTTTCATTTCACCATTCTAGAAAGGACCCAACGTTTTTCTTCATGATTCATCACAAAAATCTATGGTCTGTCTGGATTCTATATGAATGGACTTGAGATCTCTTCTATTTTGCACACAAAAGTTGTTCAAAGCCAATGGCTTGCAAGTTCTTGTTCCCTCCTTTACTAGCTTCGTTTCTTATACTCTTAACCCTTTTCACCTCTTCATTTGCTTCAGATGATTCTCTCACTAATTTCTTCCCTACCAAAGCCATTTGCAAATTGACCTCAAATCCTTCTTACTGCATCACTGTGCTTAAACAATCCAGAGATGGTAATATCTACGACTCTGGCCGGTTCTCGATTCGTCGTTCTTTATCTAAAGCCACTAGATTCCTTGACTTGATTGAGAAGCATCTTCAGAACTCTTCAACTCTACCAAATTCAATCATCGGAGCTCTTAAAGATTGTCAGTACCTTGCTCAACTGAATATGAATTTCTTGTCTAATTCGTTTCGGGCTGTTAATGGCACTGATAGAAAGCTAACTTACTCAAAAGCTGATTATATCCAAAGTCTTCTTAGTGCCATCTTAACCAACATTGATACTTGTCTTGATGGTCTAAACACTGTAGCCTCAGGTTCTAGTTTGGAGAAAGATCTATTAGCCCCACTTATTGATTGTACCAAATCATACAGCCTTTCACTTGATCTTTTTACCAAGGGTTGGGTGCCTAGGAGGAATAGAAACAGAACACTTGAGCACCCTGGGAAAAAACACCTGCAGTTTAGGAAAGGACCGTTGCCGTTGAGAATGTCGAGACACGACCGTGCTGTTTACAACTCGGTGGCAAATCGGAGAAAGCTTTCGTCTTCTTCAGATGATGGAGTGTTGGTTAATGGTGTTGTAGTTGTGAGTCAAGATGGTCAAGGAGACTTCCTTAACATAACGGATGCTATTAATGCAGCTCCAAACAACTCTCTTGCTTCTGATGGCTACTTCTTGATTTACATTACGGCTGGTGTTTATCAAGAGTATGTGTCTGTTCCTTCAAAGAAAAAGTACTTGTTGATGATTGGAGATGGTATAAATCAAACTATTATCACAGGCAACCGGAGTGTCGCTGACGGATGGACGACTTTCAACAGTGCAACCTTCGGTTAGTATTGAAATACGAATCAATATTCACCTATCTTTATGCATTGCGCTCTTATCTTATTGCACAAATGGTTAAAATTAAATATATAAAGTATAATTAGACTATCCATACAATTTACAAAGATAAATATGACCTAGGATATTTGGAGTATTTTTCTTGAAATACTTAGCTAGTTGGAAATATAGGTATGTTTTTAGTTAATGTTTAGAGATACTTGTAAATCTAATAATTGGATCCACAATATTAGAATATATATATATATTAACATTTTTAAAAAATGACAAATATAGCAAAATCTTGTTGAAGTATAGAATTCTATAATTGATAGATCATGTTGTAAATGTTGGTCTATCGATGATTTATAAATTGTTAGTGATTATAATAAACACCTTCACCATTTCTCAATTTTTTCAAACTACTTTGTTATAATTACTCTGGTTCATATAAAATCACTCAATAACCCTAAGGATCTTATAGGAAAGAAAACAATAATTTTATTTAAAGAGCATTTTTTTGTAATATTATTTAACATTCTTATATCTAGCAAATCATCTTACTATGACAGACACGTTAATTTAAAGTTTAAACAAAAATTTGACACATTTTATTTTAAAGAGAGATTTATGCACTTTTGTTTATGAACTAAAATAGACACAAATAAAATCACTTTTTCAATTAACTTGATCAGCAGTTTCTTTCTTATTAACAAAACTTCTAGATAGTTCTCATTTAATCCGGCAGGTTTCAAAATTAAAATTTATTTTACTATCTCAAACTAGGCATAAATTTGAATTTACAAATATGTATATAAATATTTATATGCTCTTCATAAAGAGTTTGCCAAACTAACATTTTATTTCAAACAATTTGTATGAAAGAATGTTCAAATGGAAATGACTTTTTCTTTGGAAGAACATTTTATGTTTAAAGATTTTCATATTTGATTTTGACATGAAAAATGCAGCTGTAGCAGCAGAAGGATTCATGGCAGTAAACATAACCATCCAAAACACAGCTGGAGCAATCAAAGGCCAAGCAGTTGCATTAAGAAGTGGAGCTGATATGTGTGTTTTTTACTCCTGCAGTTTTGAAGGCTTTCAAGACACCTTATACACTCATTCCCTCAGGCAATTCTTTAGAGAATGTGACATTTACGGCACTGTTGACTTCATTTTTGGAAATGCTGCTGTTGTCTTCCAAAACTGTAACATATATCCCCGTCTCCCCCGTCCCGGTCAGGCCAACATGATCACCGCCCAAGGCCGATCCGATCCTAACCAAAACACCGGGACTTCGATCCACAACTGCACCATCCGAGCCACCCCCGAGTTGGCTGCAAGTAGTAGCTATATGAACAAGACATATTTGGGAAGGCCATGGAAACAATACTCAAGAACAGTGTATATGCAAACATTTATTGATGGATTTGTAAATCCTAAAGGTTGGGATCCATGGACAGGAGAATATTTGAGCACATTATATTATGGAGAGTATAATAATACAGGAGGTGGATCAGATACAAAGAACAGAGTTACTTGGGCAGGTTATCATGTGATTAATAATGTAACCGATGCTGCTAATTTTACAATTTCAAACTTCTTGGTTGGGGATGCGTGGTTGCCTCCAACTTGGGTGCCTTACATGGGAGGATTCGTATAAGTGGTCAATATCTATCATAATCTAGAAGTTAAAGGTATGATCTTCCATCACACAATTGTCCAGAAAAAAATTAAAATATTGTATTTTTAGTTGAACTAAGGCTATTTTTTTTTTTCGTTTGCAAGTTAATAATATGATTGAGGGTAAATATATATTTATTTACTCCCTCATGTTTCAA

mRNA sequence

ATGGCTTGCAAGTTCTTGTTCCCTCCTTTACTAGCTTCGTTTCTTATACTCTTAACCCTTTTCACCTCTTCATTTGCTTCAGATGATTCTCTCACTAATTTCTTCCCTACCAAAGCCATTTGCAAATTGACCTCAAATCCTTCTTACTGCATCACTGTGCTTAAACAATCCAGAGATGGTAATATCTACGACTCTGGCCGGTTCTCGATTCGTCGTTCTTTATCTAAAGCCACTAGATTCCTTGACTTGATTGAGAAGCATCTTCAGAACTCTTCAACTCTACCAAATTCAATCATCGGAGCTCTTAAAGATTGTCAGTACCTTGCTCAACTGAATATGAATTTCTTGTCTAATTCGTTTCGGGCTGTTAATGGCACTGATAGAAAGCTAACTTACTCAAAAGCTGATTATATCCAAAGTCTTCTTAGTGCCATCTTAACCAACATTGATACTTGTCTTGATGGTCTAAACACTGTAGCCTCAGGTTCTAGTTTGGAGAAAGATCTATTAGCCCCACTTATTGATTGTACCAAATCATACAGCCTTTCACTTGATCTTTTTACCAAGGGTTGGGTGCCTAGGAGGAATAGAAACAGAACACTTGAGCACCCTGGGAAAAAACACCTGCAGTTTAGGAAAGGACCGTTGCCGTTGAGAATGTCGAGACACGACCGTGCTGTTTACAACTCGGTGGCAAATCGGAGAAAGCTTTCGTCTTCTTCAGATGATGGAGTGTTGGTTAATGGTGTTGTAGTTGTGAGTCAAGATGGTCAAGGAGACTTCCTTAACATAACGGATGCTATTAATGCAGCTCCAAACAACTCTCTTGCTTCTGATGGCTACTTCTTGATTTACATTACGGCTGGTGTTTATCAAGAGTATGTGTCTGTTCCTTCAAAGAAAAAGTACTTGTTGATGATTGGAGATGGTATAAATCAAACTATTATCACAGGCAACCGGAGTGTCGCTGACGGATGGACGACTTTCAACAGTGCAACCTTCGCTGTAGCAGCAGAAGGATTCATGGCAGTAAACATAACCATCCAAAACACAGCTGGAGCAATCAAAGGCCAAGCAGTTGCATTAAGAAGTGGAGCTGATATGTGTGTTTTTTACTCCTGCAGTTTTGAAGGCTTTCAAGACACCTTATACACTCATTCCCTCAGGCAATTCTTTAGAGAATGTGACATTTACGGCACTGTTGACTTCATTTTTGGAAATGCTGCTGTTGTCTTCCAAAACTGTAACATATATCCCCGTCTCCCCCGTCCCGGTCAGGCCAACATGATCACCGCCCAAGGCCGATCCGATCCTAACCAAAACACCGGGACTTCGATCCACAACTGCACCATCCGAGCCACCCCCGAGTTGGCTGCAAGTAGTAGCTATATGAACAAGACATATTTGGGAAGGCCATGGAAACAATACTCAAGAACAGTGTATATGCAAACATTTATTGATGGATTTGTAAATCCTAAAGGTTGGGATCCATGGACAGGAGAATATTTGAGCACATTATATTATGGAGAGTATAATAATACAGGAGGTGGATCAGATACAAAGAACAGAGTTACTTGGGCAGGTTATCATGTGATTAATAATGTAACCGATGCTGCTAATTTTACAATTTCAAACTTCTTGGTTGGGGATGCGTGGTTGCCTCCAACTTGGGTGCCTTACATGGGAGGATTCGTATAA

Coding sequence (CDS)

ATGGCTTGCAAGTTCTTGTTCCCTCCTTTACTAGCTTCGTTTCTTATACTCTTAACCCTTTTCACCTCTTCATTTGCTTCAGATGATTCTCTCACTAATTTCTTCCCTACCAAAGCCATTTGCAAATTGACCTCAAATCCTTCTTACTGCATCACTGTGCTTAAACAATCCAGAGATGGTAATATCTACGACTCTGGCCGGTTCTCGATTCGTCGTTCTTTATCTAAAGCCACTAGATTCCTTGACTTGATTGAGAAGCATCTTCAGAACTCTTCAACTCTACCAAATTCAATCATCGGAGCTCTTAAAGATTGTCAGTACCTTGCTCAACTGAATATGAATTTCTTGTCTAATTCGTTTCGGGCTGTTAATGGCACTGATAGAAAGCTAACTTACTCAAAAGCTGATTATATCCAAAGTCTTCTTAGTGCCATCTTAACCAACATTGATACTTGTCTTGATGGTCTAAACACTGTAGCCTCAGGTTCTAGTTTGGAGAAAGATCTATTAGCCCCACTTATTGATTGTACCAAATCATACAGCCTTTCACTTGATCTTTTTACCAAGGGTTGGGTGCCTAGGAGGAATAGAAACAGAACACTTGAGCACCCTGGGAAAAAACACCTGCAGTTTAGGAAAGGACCGTTGCCGTTGAGAATGTCGAGACACGACCGTGCTGTTTACAACTCGGTGGCAAATCGGAGAAAGCTTTCGTCTTCTTCAGATGATGGAGTGTTGGTTAATGGTGTTGTAGTTGTGAGTCAAGATGGTCAAGGAGACTTCCTTAACATAACGGATGCTATTAATGCAGCTCCAAACAACTCTCTTGCTTCTGATGGCTACTTCTTGATTTACATTACGGCTGGTGTTTATCAAGAGTATGTGTCTGTTCCTTCAAAGAAAAAGTACTTGTTGATGATTGGAGATGGTATAAATCAAACTATTATCACAGGCAACCGGAGTGTCGCTGACGGATGGACGACTTTCAACAGTGCAACCTTCGCTGTAGCAGCAGAAGGATTCATGGCAGTAAACATAACCATCCAAAACACAGCTGGAGCAATCAAAGGCCAAGCAGTTGCATTAAGAAGTGGAGCTGATATGTGTGTTTTTTACTCCTGCAGTTTTGAAGGCTTTCAAGACACCTTATACACTCATTCCCTCAGGCAATTCTTTAGAGAATGTGACATTTACGGCACTGTTGACTTCATTTTTGGAAATGCTGCTGTTGTCTTCCAAAACTGTAACATATATCCCCGTCTCCCCCGTCCCGGTCAGGCCAACATGATCACCGCCCAAGGCCGATCCGATCCTAACCAAAACACCGGGACTTCGATCCACAACTGCACCATCCGAGCCACCCCCGAGTTGGCTGCAAGTAGTAGCTATATGAACAAGACATATTTGGGAAGGCCATGGAAACAATACTCAAGAACAGTGTATATGCAAACATTTATTGATGGATTTGTAAATCCTAAAGGTTGGGATCCATGGACAGGAGAATATTTGAGCACATTATATTATGGAGAGTATAATAATACAGGAGGTGGATCAGATACAAAGAACAGAGTTACTTGGGCAGGTTATCATGTGATTAATAATGTAACCGATGCTGCTAATTTTACAATTTCAAACTTCTTGGTTGGGGATGCGTGGTTGCCTCCAACTTGGGTGCCTTACATGGGAGGATTCGTATAA

Protein sequence

MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAANFTISNFLVGDAWLPPTWVPYMGGFV*
BLAST of Csa3G890080 vs. Swiss-Prot
Match: PME20_ARATH (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=PME20 PE=2 SV=2)

HSP 1 Score: 636.7 bits (1641), Expect = 2.4e-181
Identity = 327/570 (57.37%), Postives = 413/570 (72.46%), Query Frame = 1

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60
           M+ K +F   LA    L + F S  A   ++ N      IC+   +PSYC +VL  ++ G
Sbjct: 1   MSQKLMFLFTLACLSSLPSPFIS--AQIPAIGNATSPSNICRFAPDPSYCRSVLP-NQPG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLP-NSIIGALKDCQYLAQLNMNFLSNS 120
           +IY  GR S+RRSLS+A RF+ +I+  L     +   S +GAL+DC++LA L M++L +S
Sbjct: 61  DIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSS 120

Query: 121 FRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKS 180
            +  + T + L+ S+A+ + + LSA +TN  TCL+GL + AS + L  DL     + TK 
Sbjct: 121 SQTADST-KTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLF----NDTKL 180

Query: 181 YSLSLDLFTKGWVPRRNRNRTLEHPG---KKHLQFRKGPLPLRMSRHDRAVYNSVANRRK 240
           Y +SL LF+KGWVPRR R+R +  P    KK   FR G LPL+M+   RAVYN+V  RRK
Sbjct: 181 YGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVT-RRK 240

Query: 241 LSSSSDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVS 300
           L  S  D V V+ +V V Q+G G+F  I  AI AAPN +  S+GYFLIY+TAG+Y+EYV 
Sbjct: 241 LLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVE 300

Query: 301 VPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIK 360
           VP  K+Y++MIGDGINQT+ITGNRSV DGWTTFNSATF ++   F+ VNITI+NTAG  K
Sbjct: 301 VPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTK 360

Query: 361 GQAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCN 420
           GQAVALRSG D+ VFYSCSFE +QDTLYTHSLRQF+RECD+YGTVDFIFGNAAVV QNCN
Sbjct: 361 GQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN 420

Query: 421 IYPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQY 480
           +YPR PR GQ+N +TAQGR+DPNQNTGT+IH CTIR   +L A+S+Y  KTYLGRPWK+Y
Sbjct: 421 LYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDL-ATSNYTVKTYLGRPWKEY 480

Query: 481 SRTVYMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNV 540
           SRTV MQT+IDGF+ P GW+ W+G++ LSTLYY EYNNTG GSDT NRVTW GYHVI N 
Sbjct: 481 SRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NA 540

Query: 541 TDAANFTISNFLVGDAWLPPTWVPYMGGFV 566
           TDA+NFT++NFLVG+ W+  T VP++GG +
Sbjct: 541 TDASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

BLAST of Csa3G890080 vs. Swiss-Prot
Match: PME41_ARATH (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=PME41 PE=2 SV=2)

HSP 1 Score: 622.9 bits (1605), Expect = 3.5e-177
Identity = 317/575 (55.13%), Postives = 417/575 (72.52%), Query Frame = 1

Query: 10  LLASFLILLTLFTSS---FASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSG 69
           +L+  L L+TLF S    F +  +L     +  ICK T +P +C +V  Q+  G++ + G
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYG 60

Query: 70  RFSIRRSLSKATRFLDLIEKHLQ-NSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNG 129
           RFS+R+SL+++ +F   I+++L+ N++ L  S +GAL+DC+YLA L  ++L  SF  VN 
Sbjct: 61  RFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNI 120

Query: 130 T--DRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGS-SLEKDLLAPLIDCTKSYSL 189
           T   + L++SKAD IQ+LLSA LTN  TCLDG+NT AS S ++   +  PLI+ TK +S+
Sbjct: 121 TTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSV 180

Query: 190 SLDLFTKGWVPRRNR---NRTLEHPGKKHLQ------FRKGPLPLRMSRHDRAVYNSVAN 249
           SL LFTKGWVP++ +   + +  HP   H        FR G LPL+M+ H RAVY S++ 
Sbjct: 181 SLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLS- 240

Query: 250 RRKLSSSSDD--GVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVY 309
           RRKL+   +D   VLV+ +V V+Q+G G+F  IT+A+N+APN +  + GYF+IY+T+GVY
Sbjct: 241 RRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVY 300

Query: 310 QEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNT 369
           +E V +   K+YL+MIGDGIN+T++TGNR+V DGWTTFNSATFAV +  F+AVN+T +NT
Sbjct: 301 EENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNT 360

Query: 370 AGAIKGQAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVV 429
           AG  K QAVA+RS AD+ +FYSCSFE +QDTLYTHSLRQF+RECDIYGTVDFIFGNAAVV
Sbjct: 361 AGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV 420

Query: 430 FQNCNIYPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGR 489
           FQ+CN+YPR P   Q N ITAQGR+DPNQNTG SIHNCTI+   +L  SS+Y  KTYLGR
Sbjct: 421 FQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDL-VSSNYTVKTYLGR 480

Query: 490 PWKQYSRTVYMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYH 549
           PWK+YSRTV+MQ++ID  V P GW  W G++ LSTLYY EYNNTG GS T +RV W GYH
Sbjct: 481 PWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYH 540

Query: 550 VINNVTDAANFTISNFLVGDAWLPPTWVPYMGGFV 566
           VIN+ TDA NFT+ NFL+GD W+  + VPY+ G +
Sbjct: 541 VINS-TDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Csa3G890080 vs. Swiss-Prot
Match: PME7_ARATH (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PME7 PE=2 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 3.9e-176
Identity = 325/578 (56.23%), Postives = 410/578 (70.93%), Query Frame = 1

Query: 15  LILLTLFTSS-FASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRS 74
           LI L+ F  S  AS  +L+N   +  ICK T +P YC +V   S+ GN+   G FSIR+S
Sbjct: 8   LITLSFFLQSVLASSQTLSN---SSTICKTTPDPKYCKSVFPHSQ-GNVQQYGCFSIRKS 67

Query: 75  LSKATRFLDLIEKHLQNSSTLPN-SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRK--- 134
           LS++ +F+  ++++++ ++ L   ++I AL+DC++LA L M++L  SF  VN T  K   
Sbjct: 68  LSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSF 127

Query: 135 --LTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSS---LEKDLLAPLIDCTKSYSLSL 194
             L++ KAD IQ+LLSA LTN  TCL+GL T AS S+   +   +  PL++ TK   +SL
Sbjct: 128 KPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSL 187

Query: 195 DLFTKGWVPRRN----------RNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANR 254
            LFTKGWVP++           R+ +  H  K    FR G LPL+M+   +AVY S++ R
Sbjct: 188 ALFTKGWVPKKKKRAGFAWAQPRSGSSTHT-KPFRLFRNGALPLKMTEKTKAVYESLSRR 247

Query: 255 RKLSSSS----DDG--VLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITA 314
           +     S    DDG  VL++ +V VSQDG G+F NIT A+ AAPNN+  S G+FLIY+TA
Sbjct: 248 KLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTA 307

Query: 315 GVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITI 374
           G+Y+EY+S+   K+Y++MIGDGINQT++TGNRSV DGWTTFNSATFAV A  F+AVNIT 
Sbjct: 308 GIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITF 367

Query: 375 QNTAGAIKGQAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNA 434
           +NTAG  K QAVALRSGAD  +FYSCSFE +QDTLYTHSLRQF+RECD+YGTVDFIFGNA
Sbjct: 368 RNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNA 427

Query: 435 AVVFQNCNIYPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTY 494
           AVVFQNCN+YPR P P Q N ITAQGRSDPNQNTGTSI NCTI+   +L  SS+Y  KTY
Sbjct: 428 AVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDL-VSSNYTVKTY 487

Query: 495 LGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWA 554
           LGRPWK+YSRTVYMQ++IDGFV P GW  W G++ LSTLYY EYNNTG GS+T NRVTW 
Sbjct: 488 LGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWP 547

Query: 555 GYHVINNVTDAANFTISNFLVGDAWLPPTWVPYMGGFV 566
           GYHVIN+ TDAANFT++   +   W+  T VPY  G +
Sbjct: 548 GYHVINS-TDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Csa3G890080 vs. Swiss-Prot
Match: PME25_ARATH (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=PME25 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.2e-129
Identity = 254/542 (46.86%), Postives = 341/542 (62.92%), Query Frame = 1

Query: 41  CKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSI 100
           CK T  P  C T+L   +S   + Y  G+F+I++ L +A+R   +I  + +   + P S 
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 101 ----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLD 160
               IGA+ DC  L++L++N+L      +  T + +T +  +++ SLLS ++TN  TCLD
Sbjct: 143 TAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 161 GLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGK----KHLQ 220
           GL    SG +    + +P+ + T+ YS+SL L +      RN  R     GK     +  
Sbjct: 203 GLVEAKSGFAAA--IGSPMGNLTRLYSISLGLVSHAL--NRNLKRFKASKGKILGGGNST 262

Query: 221 FRKGPLPL------RMSRHDRAVYNSVANRRKLSSSSDDGVLVNGVVVVSQDGQGDFLNI 280
           +R+ PL        +   +D+    +  N  +L  +S   +LV+  V+V      +F  I
Sbjct: 263 YRE-PLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFTTI 322

Query: 281 TDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVAD 340
           TDAI AAPNN+   DGYF+IY   GVY+EY+ VP  KK L+++GDGIN+TIITGN +V D
Sbjct: 323 TDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNVVD 382

Query: 341 GWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGFQDTLY 400
           GWTT+N ++FAV  E FMAV++T +NTAG  K QAVALR+ A+   FY CSFEG+QDTLY
Sbjct: 383 GWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLY 442

Query: 401 THSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQANMITAQGRSDPNQNTGT 460
            HSLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P   Q N ITA GR DPNQNTG 
Sbjct: 443 VHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGI 502

Query: 461 SIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGEY-L 520
           SI NCTI+A P+LAA       T+LGRPWK YSRTV+MQ++I   V P GW  W G   L
Sbjct: 503 SIINCTIKAAPDLAAEPK-SAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGL 562

Query: 521 STLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAANFTISNFLVGDAWLPPTWVPYMGG 566
            T+YYGEY+N G G++T  RV W GY+++ N+ +A NFT+ NF +GD WLP T +P+ GG
Sbjct: 563 DTIYYGEYSNFGPGANTNQRVQWLGYNLL-NLAEAMNFTVYNFTMGDTWLPQTDIPFYGG 616

BLAST of Csa3G890080 vs. Swiss-Prot
Match: PME47_ARATH (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=PME47 PE=2 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 7.8e-124
Identity = 246/549 (44.81%), Postives = 329/549 (59.93%), Query Frame = 1

Query: 36  PTKAICKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHLQNSST 95
           P    CK T  P  C T+L   +S   + Y  G+F++++ L +A R   +I +  Q    
Sbjct: 83  PPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEA 142

Query: 96  LPNSI----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNI 155
            P +     + A+ DC  LA+L++ +L      +   +  +T +  D + SLL  ++TN 
Sbjct: 143 DPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAEL-MTAALVDRVTSLLGGVVTNQ 202

Query: 156 DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGKKHL 215
            TCLDGL    SG +    +  PL + T+ YS+SL L +      RN  R   + G K  
Sbjct: 203 QTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHAL--NRNLKR---YKGSKGK 262

Query: 216 QFRKGPLPLRMSRH----------DRAVYNSVANRR--KLSSSSDDGVLVNGVVVVSQDG 275
            F  G  P+R              D+      ANR   +L  +S   +LV   V V    
Sbjct: 263 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 322

Query: 276 QGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIIT 335
             +F  IT+A+ AAPN++    GYF+IY  AG+Y+EYV + +KK+ +++IGDGIN+TII+
Sbjct: 323 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 382

Query: 336 GNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFE 395
           GN S  DGWTT+NS+TFAV  + F+AV++T +NTAG  K QAVA+R+ AD   FY CSFE
Sbjct: 383 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 442

Query: 396 GFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQANMITAQGRSD 455
           G+QDTLY HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P   Q N +TA GR+D
Sbjct: 443 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 502

Query: 456 PNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDP 515
           PNQ TG SI NCTI A P+LAA       T+LGRPWK YSRTVY+Q++I   V P GW  
Sbjct: 503 PNQKTGISIINCTIGAAPDLAADPK-STMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 562

Query: 516 WTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAANFTISNFLVGDAWLPPT 566
           W G   L T+ YGEY+N G G+DT  RV W+GY ++ N+  A NFT+ NF +GD WLP T
Sbjct: 563 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQT 621

BLAST of Csa3G890080 vs. TrEMBL
Match: A0A0A0LHM0_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G890080 PE=4 SV=1)

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 1

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180
           RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240
           SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300
           SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK
Sbjct: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360
           KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360

Query: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480
           LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540
           YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN
Sbjct: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540

Query: 541 FTISNFLVGDAWLPPTWVPYMGGFV 566
           FTISNFLVGDAWLPPTWVPYMGGFV
Sbjct: 541 FTISNFLVGDAWLPPTWVPYMGGFV 565

BLAST of Csa3G890080 vs. TrEMBL
Match: M5XEW4_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa003578mg PE=4 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 5.7e-198
Identity = 362/569 (63.62%), Postives = 434/569 (76.27%), Query Frame = 1

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MA K       +SFLI+  L +S   +D  L    P + ICK T +PSYCI+VL   ++ 
Sbjct: 1   MASKLFSLVTFSSFLIIFNLLSSPSLADVPLNTPLPPETICKSTPHPSYCISVLPH-KNA 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           N+YD GRFS++ +LS++ +  D IEK+LQ+SS LP   I AL+DC+ LA  N++FLS+S 
Sbjct: 61  NVYDFGRFSVQHALSQSHKLFDSIEKYLQSSSALPQPAIQALEDCKLLALSNIDFLSSSL 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTV-ASGSSLEKDLLAPLIDCTKS 180
           + VN T   L+   AD  Q+LLSAILTN  TC DG+ ++ +S  S   DL  P+ + TK 
Sbjct: 121 QTVNKTSGVLSSLDADDAQTLLSAILTNHQTCSDGIESLPSSAGSAISDLSVPISNNTKL 180

Query: 181 YSLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVAN--RRKL 240
           YS+SL LFTKGWVP+ +++   + P K+  +F KG L L+MS H RA+Y++  N  RR+L
Sbjct: 181 YSVSLALFTKGWVPK-DKSGVPKRP-KRQFRFGKGRLNLKMSSHARAIYDAAINHRRRRL 240

Query: 241 SSSSDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSV 300
               D+ VLV  +VVVSQDG G+F  I +AI  APNNS+ASDGYFLIYITAGVYQEYVS+
Sbjct: 241 LQVGDEEVLVKDIVVVSQDGSGNFTTINEAIAIAPNNSVASDGYFLIYITAGVYQEYVSI 300

Query: 301 PSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKG 360
            SKKKYLL IGDGINQTIITGN SV DG TTFNSAT AV AEGFMAVNIT++NTAG  KG
Sbjct: 301 ISKKKYLLFIGDGINQTIITGNNSVGDGSTTFNSATLAVVAEGFMAVNITVRNTAGPSKG 360

Query: 361 QAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNI 420
           QAVALRSGAD+ VFYSCSFEG+QDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNI
Sbjct: 361 QAVALRSGADLSVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNI 420

Query: 421 YPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYS 480
           YPR P  GQ N ITAQGR+DPNQNTGTSIHNCTI+   +L ASS++  KTYLGRPWK+YS
Sbjct: 421 YPRQPNKGQFNPITAQGRTDPNQNTGTSIHNCTIKPAADL-ASSNFTVKTYLGRPWKEYS 480

Query: 481 RTVYMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVT 540
           RTVYMQTF+D F++P GW  W+G++ LSTLYY EYNNTG GS T NRVTW+GYHVIN+ T
Sbjct: 481 RTVYMQTFMDSFIDPAGWHDWSGDFALSTLYYAEYNNTGPGSSTANRVTWSGYHVINS-T 540

Query: 541 DAANFTISNFLVGDAWLPPTWVPYMGGFV 566
           DAANFT+SNFL+GD WLP T VPY GG +
Sbjct: 541 DAANFTVSNFLLGDNWLPQTGVPYTGGLI 564

BLAST of Csa3G890080 vs. TrEMBL
Match: M5VIT6_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa003639mg PE=4 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 6.9e-196
Identity = 349/559 (62.43%), Postives = 435/559 (77.82%), Query Frame = 1

Query: 10  LLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG-NIYDSGRF 69
           ++ASF IL  LF+S   + D+ ++      ICK T +PS+C +VL  ++   N+Y  GRF
Sbjct: 7   IIASFFILFPLFSSLSLAADNSSSPVSAGTICKSTPDPSFCKSVLPHNQTAENVYYYGRF 66

Query: 70  SIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDR 129
           S+R+SLS++ +FL+L++K+L+  S+L  + + AL+DC+ LA LNM+FL NSF  VN T +
Sbjct: 67  SVRKSLSQSRKFLNLVDKYLKRRSSLSLTAVRALEDCRLLAGLNMDFLLNSFETVNKTSK 126

Query: 130 KLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFT 189
            L+  KAD +Q+ LSAILTN  TCLDG+ + AS  SL+  L AP  + TK YS+SL LFT
Sbjct: 127 TLSNLKADDVQTSLSAILTNQQTCLDGIQSTASAWSLKTGLSAP--NDTKFYSVSLALFT 186

Query: 190 KGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSS-DDGVLV 249
           KGWVP++ ++ T + P +K   F+ G LPL+MS   RAVY  V  R+ L + + DDGVLV
Sbjct: 187 KGWVPKKKKSATWK-PTRKQRGFKNGRLPLKMSSRTRAVYEGVTKRKLLQTETEDDGVLV 246

Query: 250 NGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSKKKYLLMI 309
           + VV VSQ+G G+F  I DAI AAPNNS +++GYFLIY+TAGVY+EYVS+   K+YL+M+
Sbjct: 247 SEVVTVSQNGTGNFSTINDAIAAAPNNSASTNGYFLIYVTAGVYEEYVSIAKNKRYLMMV 306

Query: 310 GDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGAD 369
           GDGINQTI+TGNRSV DGWTTFNSATFAV A GF+AVN+T +NTAGAIK QAVA+R+GAD
Sbjct: 307 GDGINQTILTGNRSVVDGWTTFNSATFAVTAPGFVAVNMTFRNTAGAIKHQAVAVRNGAD 366

Query: 370 MCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQA 429
           +  FYSCSFE +QDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVFQNCN+YPRLP  GQ 
Sbjct: 367 LSTFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYPRLPMSGQF 426

Query: 430 NMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTVYMQTFID 489
           N ITAQGR+DPNQNTGTSIHNCTIRA  +LA+S+S   KTYLGRPWK+YSRTVYMQ++ID
Sbjct: 427 NAITAQGRTDPNQNTGTSIHNCTIRAADDLASSNS-STKTYLGRPWKEYSRTVYMQSYID 486

Query: 490 GFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAANFTISNF 549
             V+P GW  W GE+ LSTLYY EYNNTG GS+T +RVTW GYHVI N TDAANFT++NF
Sbjct: 487 SVVDPAGWRAWDGEFALSTLYYAEYNNTGPGSNTTSRVTWPGYHVI-NATDAANFTVTNF 546

Query: 550 LVGDAWLPPTWVPYMGGFV 566
           L+GD WLP T VPY GG +
Sbjct: 547 LLGDDWLPQTGVPYTGGLI 560

BLAST of Csa3G890080 vs. TrEMBL
Match: A0A067K8R5_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_13960 PE=4 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 5.0e-194
Identity = 338/566 (59.72%), Postives = 433/566 (76.50%), Query Frame = 1

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MA  F+F      FL++L LF+S+  +D    N      +CK T +PSYC ++L  ++  
Sbjct: 1   MASTFIF------FLLVLALFSSATLADFPPNNTLSPGTVCKSTPDPSYCKSILP-NQTS 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           N+Y  GRFS+R+SLS++ +FL L++K+L   S+L    I AL+DC+ LA LNM+FL ++F
Sbjct: 61  NVYGYGRFSVRKSLSQSLKFLKLVDKYLSRRSSLTTPAIRALEDCKLLAGLNMDFLLSAF 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180
           + VN T + L+  KAD +Q+LLSAILTN  TC+DGL   AS  S++  L  PL + TK Y
Sbjct: 121 QTVNTTSKTLSSLKADDVQTLLSAILTNQQTCIDGLQATASAWSIKNGLSVPLSNDTKLY 180

Query: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240
           S+SL LFTKGWVPR+ +  T + P  +  +F+ G LPL+MS   R++Y SV+ R+ L + 
Sbjct: 181 SVSLALFTKGWVPRKKKGLTWQ-PTSQQAKFKNGRLPLKMSSRTRSIYESVSRRKLLQTQ 240

Query: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300
            D  VLV+ +V VSQ+G GDF +I+DA+ +APNN+  S+GYF+I+I+AG+Y+EYVS+P  
Sbjct: 241 DDGAVLVSDIVTVSQNGTGDFTSISDAVASAPNNTDGSNGYFMIFISAGIYEEYVSIPKN 300

Query: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360
           KKYL+M+GDGINQTIITGNRSV DGWTTFNSATFAV A  F+AVNITI+NTAG  K QAV
Sbjct: 301 KKYLMMVGDGINQTIITGNRSVVDGWTTFNSATFAVVAPNFVAVNITIRNTAGPEKHQAV 360

Query: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           ALRSGAD+  FYSCSFEG+QDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVFQNCN+YPR
Sbjct: 361 ALRSGADLSTFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYPR 420

Query: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480
           LP  GQ N ITAQGR+DPNQNTGTSIHNC+IRA  +LA+S++ + +T+LGRPWK YSRTV
Sbjct: 421 LPMTGQFNAITAQGRTDPNQNTGTSIHNCSIRAADDLASSNAKI-QTFLGRPWKNYSRTV 480

Query: 481 YMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAA 540
           YM++F+D  +NP GW  W+G++ LSTLYY EYNN G G++T NRVTW GYHVIN+ TDAA
Sbjct: 481 YMESFMDSLINPAGWQIWSGDFALSTLYYAEYNNFGAGANTTNRVTWEGYHVINS-TDAA 540

Query: 541 NFTISNFLVGDAWLPPTWVPYMGGFV 566
           NFT+SNFL+GD W+P   VP+  G V
Sbjct: 541 NFTVSNFLLGDNWIPQAGVPFSSGLV 556

BLAST of Csa3G890080 vs. TrEMBL
Match: M5XRB3_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa003581mg PE=4 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 9.4e-193
Identity = 355/569 (62.39%), Postives = 427/569 (75.04%), Query Frame = 1

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MA K       +SFLI+    +S   +D  L    P + ICK T +PSYCI+VL   ++ 
Sbjct: 1   MASKLFSLATFSSFLIIFHSLSSPSLADVPLDTPLPPETICKSTPHPSYCISVLPH-KNA 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           N+YD GRFS++ +LS++    D IE HLQ  S LP   I AL+DC+ L  LN++FLS+S 
Sbjct: 61  NVYDFGRFSVQHALSQSHNLFDSIENHLQLGSVLPQPAIQALEDCKLLTLLNIDFLSSSL 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTV-ASGSSLEKDLLAPLIDCTKS 180
             VN T   L    AD +Q+LLSAILTN  TC DGL ++ +S  SL  DL A + + ++ 
Sbjct: 121 ETVNKTSGVLASLDADDVQTLLSAILTNQQTCSDGLESLPSSAGSLINDLSASISNNSQL 180

Query: 181 YSLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVAN--RRKL 240
           YS+SL LFTKGWVP+ ++N   + P K+HLQF KG L L+MS   RA+Y++  N  RR+L
Sbjct: 181 YSVSLALFTKGWVPK-DKNGVPKQP-KRHLQFGKGRLNLKMSSQARAIYDAAINHRRRRL 240

Query: 241 SSSSDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSV 300
               D+ VLV G+VVVSQDG G+F  I  AI AAPNNS+AS GYFLIY+TAGVYQEYVS+
Sbjct: 241 LQVGDEEVLVKGIVVVSQDGSGNFTTINAAIAAAPNNSVASGGYFLIYVTAGVYQEYVSI 300

Query: 301 PSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKG 360
            S KKYLLMIGDGINQTIITGN SV DG TTFNSAT AV   GF+AVNIT++NTAG  KG
Sbjct: 301 ASNKKYLLMIGDGINQTIITGNNSVGDGSTTFNSATLAVTGLGFVAVNITVRNTAGPSKG 360

Query: 361 QAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNI 420
           QAVALRSGAD+ VFYSCSFEG+QDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVFQNCN+
Sbjct: 361 QAVALRSGADLSVFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNL 420

Query: 421 YPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYS 480
           YPR P  GQ+N ITAQGR+DPNQNTGTSIHNCTI+ TP+L ASS++  KTYLGRPWK+YS
Sbjct: 421 YPRQPNKGQSNAITAQGRTDPNQNTGTSIHNCTIKPTPDL-ASSNFTVKTYLGRPWKEYS 480

Query: 481 RTVYMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVT 540
           RTVYMQTF+   ++P GW  W+G++ LSTLYY EYNNTG GS+T NRVTW GYHVIN+ T
Sbjct: 481 RTVYMQTFMASLIDPAGWLAWSGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINS-T 540

Query: 541 DAANFTISNFLVGDAWLPPTWVPYMGGFV 566
            AANFT++NFL+GD WL  T VPY GG +
Sbjct: 541 VAANFTVTNFLMGDNWLAATGVPYTGGLI 564

BLAST of Csa3G890080 vs. TAIR10
Match: AT2G47550.1 (AT2G47550.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 636.7 bits (1641), Expect = 1.3e-182
Identity = 327/570 (57.37%), Postives = 413/570 (72.46%), Query Frame = 1

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60
           M+ K +F   LA    L + F S  A   ++ N      IC+   +PSYC +VL  ++ G
Sbjct: 1   MSQKLMFLFTLACLSSLPSPFIS--AQIPAIGNATSPSNICRFAPDPSYCRSVLP-NQPG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLP-NSIIGALKDCQYLAQLNMNFLSNS 120
           +IY  GR S+RRSLS+A RF+ +I+  L     +   S +GAL+DC++LA L M++L +S
Sbjct: 61  DIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSS 120

Query: 121 FRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKS 180
            +  + T + L+ S+A+ + + LSA +TN  TCL+GL + AS + L  DL     + TK 
Sbjct: 121 SQTADST-KTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLF----NDTKL 180

Query: 181 YSLSLDLFTKGWVPRRNRNRTLEHPG---KKHLQFRKGPLPLRMSRHDRAVYNSVANRRK 240
           Y +SL LF+KGWVPRR R+R +  P    KK   FR G LPL+M+   RAVYN+V  RRK
Sbjct: 181 YGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVT-RRK 240

Query: 241 LSSSSDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVS 300
           L  S  D V V+ +V V Q+G G+F  I  AI AAPN +  S+GYFLIY+TAG+Y+EYV 
Sbjct: 241 LLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVE 300

Query: 301 VPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIK 360
           VP  K+Y++MIGDGINQT+ITGNRSV DGWTTFNSATF ++   F+ VNITI+NTAG  K
Sbjct: 301 VPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTK 360

Query: 361 GQAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCN 420
           GQAVALRSG D+ VFYSCSFE +QDTLYTHSLRQF+RECD+YGTVDFIFGNAAVV QNCN
Sbjct: 361 GQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN 420

Query: 421 IYPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQY 480
           +YPR PR GQ+N +TAQGR+DPNQNTGT+IH CTIR   +L A+S+Y  KTYLGRPWK+Y
Sbjct: 421 LYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDL-ATSNYTVKTYLGRPWKEY 480

Query: 481 SRTVYMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNV 540
           SRTV MQT+IDGF+ P GW+ W+G++ LSTLYY EYNNTG GSDT NRVTW GYHVI N 
Sbjct: 481 SRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NA 540

Query: 541 TDAANFTISNFLVGDAWLPPTWVPYMGGFV 566
           TDA+NFT++NFLVG+ W+  T VP++GG +
Sbjct: 541 TDASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

BLAST of Csa3G890080 vs. TAIR10
Match: AT4G02330.1 (AT4G02330.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 622.9 bits (1605), Expect = 2.0e-178
Identity = 317/575 (55.13%), Postives = 417/575 (72.52%), Query Frame = 1

Query: 10  LLASFLILLTLFTSS---FASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSG 69
           +L+  L L+TLF S    F +  +L     +  ICK T +P +C +V  Q+  G++ + G
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYG 60

Query: 70  RFSIRRSLSKATRFLDLIEKHLQ-NSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNG 129
           RFS+R+SL+++ +F   I+++L+ N++ L  S +GAL+DC+YLA L  ++L  SF  VN 
Sbjct: 61  RFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNI 120

Query: 130 T--DRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGS-SLEKDLLAPLIDCTKSYSL 189
           T   + L++SKAD IQ+LLSA LTN  TCLDG+NT AS S ++   +  PLI+ TK +S+
Sbjct: 121 TTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSV 180

Query: 190 SLDLFTKGWVPRRNR---NRTLEHPGKKHLQ------FRKGPLPLRMSRHDRAVYNSVAN 249
           SL LFTKGWVP++ +   + +  HP   H        FR G LPL+M+ H RAVY S++ 
Sbjct: 181 SLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLS- 240

Query: 250 RRKLSSSSDD--GVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVY 309
           RRKL+   +D   VLV+ +V V+Q+G G+F  IT+A+N+APN +  + GYF+IY+T+GVY
Sbjct: 241 RRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVY 300

Query: 310 QEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNT 369
           +E V +   K+YL+MIGDGIN+T++TGNR+V DGWTTFNSATFAV +  F+AVN+T +NT
Sbjct: 301 EENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNT 360

Query: 370 AGAIKGQAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVV 429
           AG  K QAVA+RS AD+ +FYSCSFE +QDTLYTHSLRQF+RECDIYGTVDFIFGNAAVV
Sbjct: 361 AGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV 420

Query: 430 FQNCNIYPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGR 489
           FQ+CN+YPR P   Q N ITAQGR+DPNQNTG SIHNCTI+   +L  SS+Y  KTYLGR
Sbjct: 421 FQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDL-VSSNYTVKTYLGR 480

Query: 490 PWKQYSRTVYMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYH 549
           PWK+YSRTV+MQ++ID  V P GW  W G++ LSTLYY EYNNTG GS T +RV W GYH
Sbjct: 481 PWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYH 540

Query: 550 VINNVTDAANFTISNFLVGDAWLPPTWVPYMGGFV 566
           VIN+ TDA NFT+ NFL+GD W+  + VPY+ G +
Sbjct: 541 VINS-TDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Csa3G890080 vs. TAIR10
Match: AT1G02810.1 (AT1G02810.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 619.4 bits (1596), Expect = 2.2e-177
Identity = 325/578 (56.23%), Postives = 410/578 (70.93%), Query Frame = 1

Query: 15  LILLTLFTSS-FASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRS 74
           LI L+ F  S  AS  +L+N   +  ICK T +P YC +V   S+ GN+   G FSIR+S
Sbjct: 8   LITLSFFLQSVLASSQTLSN---SSTICKTTPDPKYCKSVFPHSQ-GNVQQYGCFSIRKS 67

Query: 75  LSKATRFLDLIEKHLQNSSTLPN-SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRK--- 134
           LS++ +F+  ++++++ ++ L   ++I AL+DC++LA L M++L  SF  VN T  K   
Sbjct: 68  LSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSF 127

Query: 135 --LTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSS---LEKDLLAPLIDCTKSYSLSL 194
             L++ KAD IQ+LLSA LTN  TCL+GL T AS S+   +   +  PL++ TK   +SL
Sbjct: 128 KPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSL 187

Query: 195 DLFTKGWVPRRN----------RNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANR 254
            LFTKGWVP++           R+ +  H  K    FR G LPL+M+   +AVY S++ R
Sbjct: 188 ALFTKGWVPKKKKRAGFAWAQPRSGSSTHT-KPFRLFRNGALPLKMTEKTKAVYESLSRR 247

Query: 255 RKLSSSS----DDG--VLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITA 314
           +     S    DDG  VL++ +V VSQDG G+F NIT A+ AAPNN+  S G+FLIY+TA
Sbjct: 248 KLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTA 307

Query: 315 GVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITI 374
           G+Y+EY+S+   K+Y++MIGDGINQT++TGNRSV DGWTTFNSATFAV A  F+AVNIT 
Sbjct: 308 GIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITF 367

Query: 375 QNTAGAIKGQAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNA 434
           +NTAG  K QAVALRSGAD  +FYSCSFE +QDTLYTHSLRQF+RECD+YGTVDFIFGNA
Sbjct: 368 RNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNA 427

Query: 435 AVVFQNCNIYPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTY 494
           AVVFQNCN+YPR P P Q N ITAQGRSDPNQNTGTSI NCTI+   +L  SS+Y  KTY
Sbjct: 428 AVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDL-VSSNYTVKTY 487

Query: 495 LGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWA 554
           LGRPWK+YSRTVYMQ++IDGFV P GW  W G++ LSTLYY EYNNTG GS+T NRVTW 
Sbjct: 488 LGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWP 547

Query: 555 GYHVINNVTDAANFTISNFLVGDAWLPPTWVPYMGGFV 566
           GYHVIN+ TDAANFT++   +   W+  T VPY  G +
Sbjct: 548 GYHVINS-TDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Csa3G890080 vs. TAIR10
Match: AT3G10720.2 (AT3G10720.2 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 464.9 bits (1195), Expect = 7.0e-131
Identity = 254/542 (46.86%), Postives = 341/542 (62.92%), Query Frame = 1

Query: 41  CKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSI 100
           CK T  P  C T+L   +S   + Y  G+F+I++ L +A+R   +I  + +   + P S 
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 101 ----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLD 160
               IGA+ DC  L++L++N+L      +  T + +T +  +++ SLLS ++TN  TCLD
Sbjct: 143 TAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 161 GLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGK----KHLQ 220
           GL    SG +    + +P+ + T+ YS+SL L +      RN  R     GK     +  
Sbjct: 203 GLVEAKSGFAAA--IGSPMGNLTRLYSISLGLVSHAL--NRNLKRFKASKGKILGGGNST 262

Query: 221 FRKGPLPL------RMSRHDRAVYNSVANRRKLSSSSDDGVLVNGVVVVSQDGQGDFLNI 280
           +R+ PL        +   +D+    +  N  +L  +S   +LV+  V+V      +F  I
Sbjct: 263 YRE-PLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFTTI 322

Query: 281 TDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVAD 340
           TDAI AAPNN+   DGYF+IY   GVY+EY+ VP  KK L+++GDGIN+TIITGN +V D
Sbjct: 323 TDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNVVD 382

Query: 341 GWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGFQDTLY 400
           GWTT+N ++FAV  E FMAV++T +NTAG  K QAVALR+ A+   FY CSFEG+QDTLY
Sbjct: 383 GWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLY 442

Query: 401 THSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQANMITAQGRSDPNQNTGT 460
            HSLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P   Q N ITA GR DPNQNTG 
Sbjct: 443 VHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGI 502

Query: 461 SIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGEY-L 520
           SI NCTI+A P+LAA       T+LGRPWK YSRTV+MQ++I   V P GW  W G   L
Sbjct: 503 SIINCTIKAAPDLAAEPK-SAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGL 562

Query: 521 STLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAANFTISNFLVGDAWLPPTWVPYMGG 566
            T+YYGEY+N G G++T  RV W GY+++ N+ +A NFT+ NF +GD WLP T +P+ GG
Sbjct: 563 DTIYYGEYSNFGPGANTNQRVQWLGYNLL-NLAEAMNFTVYNFTMGDTWLPQTDIPFYGG 616

BLAST of Csa3G890080 vs. TAIR10
Match: AT5G04970.1 (AT5G04970.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 445.7 bits (1145), Expect = 4.4e-125
Identity = 246/549 (44.81%), Postives = 329/549 (59.93%), Query Frame = 1

Query: 36  PTKAICKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHLQNSST 95
           P    CK T  P  C T+L   +S   + Y  G+F++++ L +A R   +I +  Q    
Sbjct: 83  PPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEA 142

Query: 96  LPNSI----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNI 155
            P +     + A+ DC  LA+L++ +L      +   +  +T +  D + SLL  ++TN 
Sbjct: 143 DPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAEL-MTAALVDRVTSLLGGVVTNQ 202

Query: 156 DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGKKHL 215
            TCLDGL    SG +    +  PL + T+ YS+SL L +      RN  R   + G K  
Sbjct: 203 QTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHAL--NRNLKR---YKGSKGK 262

Query: 216 QFRKGPLPLRMSRH----------DRAVYNSVANRR--KLSSSSDDGVLVNGVVVVSQDG 275
            F  G  P+R              D+      ANR   +L  +S   +LV   V V    
Sbjct: 263 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 322

Query: 276 QGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIIT 335
             +F  IT+A+ AAPN++    GYF+IY  AG+Y+EYV + +KK+ +++IGDGIN+TII+
Sbjct: 323 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 382

Query: 336 GNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFE 395
           GN S  DGWTT+NS+TFAV  + F+AV++T +NTAG  K QAVA+R+ AD   FY CSFE
Sbjct: 383 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 442

Query: 396 GFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQANMITAQGRSD 455
           G+QDTLY HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P   Q N +TA GR+D
Sbjct: 443 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 502

Query: 456 PNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDP 515
           PNQ TG SI NCTI A P+LAA       T+LGRPWK YSRTVY+Q++I   V P GW  
Sbjct: 503 PNQKTGISIINCTIGAAPDLAADPK-STMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 562

Query: 516 WTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAANFTISNFLVGDAWLPPT 566
           W G   L T+ YGEY+N G G+DT  RV W+GY ++ N+  A NFT+ NF +GD WLP T
Sbjct: 563 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQT 621

BLAST of Csa3G890080 vs. NCBI nr
Match: gi|449436956|ref|XP_004136258.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 [Cucumis sativus])

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 1

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180
           RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240
           SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300
           SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK
Sbjct: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360
           KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360

Query: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480
           LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540
           YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN
Sbjct: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540

Query: 541 FTISNFLVGDAWLPPTWVPYMGGFV 566
           FTISNFLVGDAWLPPTWVPYMGGFV
Sbjct: 541 FTISNFLVGDAWLPPTWVPYMGGFV 565

BLAST of Csa3G890080 vs. NCBI nr
Match: gi|659132384|ref|XP_008466169.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 539/565 (95.40%), Postives = 549/565 (97.17%), Query Frame = 1

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MACKFLFPPL   FLILLTLFTSSFASDDSLTN FPTKAICKLTSNPSYCITVLKQSRDG
Sbjct: 1   MACKFLFPPL---FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           NIYDSGRFSIRRSLSKA+RFLDLIEKHLQNSSTLP SIIGALKDCQYLAQLNMNFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180
           RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240
           SLSLDLFTKGWVPR NRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS
Sbjct: 181 SLSLDLFTKGWVPRWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300
           SDDGVLVNG+ VVSQDG+GDFLNITDAINAAPNNSLA++GYFLIYITAGVYQEYVSVPSK
Sbjct: 241 SDDGVLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360
           KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNIT QNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           ALRSGADMCVFYSCSFEG+QDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480
           LP P QANMITAQGRSDPNQNTGTSIHNCTI+ATP+LAASSS+MNKTYLGRPWKQYSRTV
Sbjct: 421 LPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540
           YMQTFIDGFVNPKGWDPW+GEYLSTLYYGEYNNTGGGSDTKNRV W GYHVINN TDAAN
Sbjct: 481 YMQTFIDGFVNPKGWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAAN 540

Query: 541 FTISNFLVGDAWLPPTWVPYMGGFV 566
           FTISNFLVGD WLPPT VPY GGFV
Sbjct: 541 FTISNFLVGDVWLPPTGVPYTGGFV 562

BLAST of Csa3G890080 vs. NCBI nr
Match: gi|659132386|ref|XP_008466170.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X2 [Cucumis melo])

HSP 1 Score: 1051.2 bits (2717), Expect = 6.5e-304
Identity = 514/537 (95.72%), Postives = 524/537 (97.58%), Query Frame = 1

Query: 29  DSLTNFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHL 88
           DSLTN FPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKA+RFLDLIEKHL
Sbjct: 9   DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 68

Query: 89  QNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 148
           QNSSTLP SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN
Sbjct: 69  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 128

Query: 149 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGKKH 208
           IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR NRNRTLEHPGKKH
Sbjct: 129 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 188

Query: 209 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGVVVVSQDGQGDFLNITDAI 268
           LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNG+ VVSQDG+GDFLNITDAI
Sbjct: 189 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 248

Query: 269 NAAPNNSLASDGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 328
           NAAPNNSLA++GYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT
Sbjct: 249 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 308

Query: 329 FNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGFQDTLYTHSL 388
           FNSATFAVAAEGFMAVNIT QNTAGAIKGQAVALRSGADMCVFYSCSFEG+QDTLYTHSL
Sbjct: 309 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 368

Query: 389 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQANMITAQGRSDPNQNTGTSIHN 448
           RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP P QANMITAQGRSDPNQNTGTSIHN
Sbjct: 369 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 428

Query: 449 CTIRATPELAASSSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGEYLSTLYY 508
           CTI+ATP+LAASSS+MNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPW+GEYLSTLYY
Sbjct: 429 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 488

Query: 509 GEYNNTGGGSDTKNRVTWAGYHVINNVTDAANFTISNFLVGDAWLPPTWVPYMGGFV 566
           GEYNNTGGGSDTKNRV W GYHVINN TDAANFTISNFLVGD WLPPT VPY GGFV
Sbjct: 489 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 545

BLAST of Csa3G890080 vs. NCBI nr
Match: gi|596153974|ref|XP_007222797.1| (hypothetical protein PRUPE_ppa003578mg [Prunus persica])

HSP 1 Score: 698.7 bits (1802), Expect = 8.1e-198
Identity = 362/569 (63.62%), Postives = 434/569 (76.27%), Query Frame = 1

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MA K       +SFLI+  L +S   +D  L    P + ICK T +PSYCI+VL   ++ 
Sbjct: 1   MASKLFSLVTFSSFLIIFNLLSSPSLADVPLNTPLPPETICKSTPHPSYCISVLPH-KNA 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           N+YD GRFS++ +LS++ +  D IEK+LQ+SS LP   I AL+DC+ LA  N++FLS+S 
Sbjct: 61  NVYDFGRFSVQHALSQSHKLFDSIEKYLQSSSALPQPAIQALEDCKLLALSNIDFLSSSL 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTV-ASGSSLEKDLLAPLIDCTKS 180
           + VN T   L+   AD  Q+LLSAILTN  TC DG+ ++ +S  S   DL  P+ + TK 
Sbjct: 121 QTVNKTSGVLSSLDADDAQTLLSAILTNHQTCSDGIESLPSSAGSAISDLSVPISNNTKL 180

Query: 181 YSLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVAN--RRKL 240
           YS+SL LFTKGWVP+ +++   + P K+  +F KG L L+MS H RA+Y++  N  RR+L
Sbjct: 181 YSVSLALFTKGWVPK-DKSGVPKRP-KRQFRFGKGRLNLKMSSHARAIYDAAINHRRRRL 240

Query: 241 SSSSDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSV 300
               D+ VLV  +VVVSQDG G+F  I +AI  APNNS+ASDGYFLIYITAGVYQEYVS+
Sbjct: 241 LQVGDEEVLVKDIVVVSQDGSGNFTTINEAIAIAPNNSVASDGYFLIYITAGVYQEYVSI 300

Query: 301 PSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKG 360
            SKKKYLL IGDGINQTIITGN SV DG TTFNSAT AV AEGFMAVNIT++NTAG  KG
Sbjct: 301 ISKKKYLLFIGDGINQTIITGNNSVGDGSTTFNSATLAVVAEGFMAVNITVRNTAGPSKG 360

Query: 361 QAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNI 420
           QAVALRSGAD+ VFYSCSFEG+QDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNI
Sbjct: 361 QAVALRSGADLSVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNI 420

Query: 421 YPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYS 480
           YPR P  GQ N ITAQGR+DPNQNTGTSIHNCTI+   +L ASS++  KTYLGRPWK+YS
Sbjct: 421 YPRQPNKGQFNPITAQGRTDPNQNTGTSIHNCTIKPAADL-ASSNFTVKTYLGRPWKEYS 480

Query: 481 RTVYMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVT 540
           RTVYMQTF+D F++P GW  W+G++ LSTLYY EYNNTG GS T NRVTW+GYHVIN+ T
Sbjct: 481 RTVYMQTFMDSFIDPAGWHDWSGDFALSTLYYAEYNNTGPGSSTANRVTWSGYHVINS-T 540

Query: 541 DAANFTISNFLVGDAWLPPTWVPYMGGFV 566
           DAANFT+SNFL+GD WLP T VPY GG +
Sbjct: 541 DAANFTVSNFLLGDNWLPQTGVPYTGGLI 564

BLAST of Csa3G890080 vs. NCBI nr
Match: gi|645224820|ref|XP_008219290.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Prunus mume])

HSP 1 Score: 694.9 bits (1792), Expect = 1.2e-196
Identity = 360/569 (63.27%), Postives = 432/569 (75.92%), Query Frame = 1

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MA K       +SFLI+  L +S   +D  L    P + ICK T +PSYCI+VL   ++ 
Sbjct: 1   MASKLFSLVTFSSFLIIFNLLSSPSLADVPLNTPLPPETICKSTPHPSYCISVLPH-KNA 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           N+YD GRFS++ +LS++ +  D IE +LQ+SS LP   I AL+DC+ LA  N++FLS+S 
Sbjct: 61  NVYDFGRFSVQHALSQSHKLFDSIENYLQSSSALPQPAIQALEDCKLLALSNIDFLSSSL 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTV-ASGSSLEKDLLAPLIDCTKS 180
           + VN T   L+   AD  Q+LLSAILTN  TC DG+ ++ +S  S   DL  P+ + TK 
Sbjct: 121 QTVNKTSGVLSSLDADDAQTLLSAILTNHQTCSDGIESLPSSAGSAISDLSVPISNNTKL 180

Query: 181 YSLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVAN--RRKL 240
           YS+SL LFTKGWVP+ +++   + P K+  +F KG L L+MS H RA+Y++  N  RR+L
Sbjct: 181 YSVSLALFTKGWVPK-DKSGVPKRP-KRQFRFGKGRLNLKMSSHARAIYDAAINHRRRRL 240

Query: 241 SSSSDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSV 300
               D+ VLV  +VVVSQDG G+F  I +AI  APNNS+ASDGYFLIYITAGVYQEYVS+
Sbjct: 241 LQVGDEEVLVKDIVVVSQDGSGNFTTINEAIAIAPNNSVASDGYFLIYITAGVYQEYVSI 300

Query: 301 PSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKG 360
            SKKKYLL IGDGINQTIITGN SV DG TTFNSAT AV AEGFMAVNIT++NTAG  KG
Sbjct: 301 ISKKKYLLFIGDGINQTIITGNNSVGDGSTTFNSATLAVVAEGFMAVNITVRNTAGPSKG 360

Query: 361 QAVALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNI 420
           QAVALRSGAD+ VFYSCSFEG+QDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVFQNCNI
Sbjct: 361 QAVALRSGADLSVFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNI 420

Query: 421 YPRLPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYS 480
           YPR P  GQ N ITAQGR+DPNQNTGTSIHNCTI+   +L ASS++  KTYLGRPWK+YS
Sbjct: 421 YPRQPNQGQFNPITAQGRTDPNQNTGTSIHNCTIKPAADL-ASSNFTVKTYLGRPWKEYS 480

Query: 481 RTVYMQTFIDGFVNPKGWDPWTGEY-LSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVT 540
           RTVYMQTF+D F++P GW  W+G++ LSTLYY EYNNTG GS T NRVTW GYHVIN+ T
Sbjct: 481 RTVYMQTFMDSFIDPAGWHDWSGDFALSTLYYAEYNNTGPGSSTANRVTWPGYHVINS-T 540

Query: 541 DAANFTISNFLVGDAWLPPTWVPYMGGFV 566
           DAANFT+SNFL+GD WLP T VPY GG +
Sbjct: 541 DAANFTVSNFLLGDNWLPQTGVPYTGGLI 564

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME20_ARATH2.4e-18157.37Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=P... [more]
PME41_ARATH3.5e-17755.13Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=P... [more]
PME7_ARATH3.9e-17656.23Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PM... [more]
PME25_ARATH1.2e-12946.86Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=P... [more]
PME47_ARATH7.8e-12444.81Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=P... [more]
Match NameE-valueIdentityDescription
A0A0A0LHM0_CUCSA0.0e+00100.00Pectinesterase OS=Cucumis sativus GN=Csa_3G890080 PE=4 SV=1[more]
M5XEW4_PRUPE5.7e-19863.62Pectinesterase OS=Prunus persica GN=PRUPE_ppa003578mg PE=4 SV=1[more]
M5VIT6_PRUPE6.9e-19662.43Pectinesterase OS=Prunus persica GN=PRUPE_ppa003639mg PE=4 SV=1[more]
A0A067K8R5_JATCU5.0e-19459.72Pectinesterase OS=Jatropha curcas GN=JCGZ_13960 PE=4 SV=1[more]
M5XRB3_PRUPE9.4e-19362.39Pectinesterase OS=Prunus persica GN=PRUPE_ppa003581mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G47550.11.3e-18257.37 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G02330.12.0e-17855.13 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G02810.12.2e-17756.23 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G10720.27.0e-13146.86 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G04970.14.4e-12544.81 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|449436956|ref|XP_004136258.1|0.0e+00100.00PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 [Cucumis sativus][more]
gi|659132384|ref|XP_008466169.1|0.0e+0095.40PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucum... [more]
gi|659132386|ref|XP_008466170.1|6.5e-30495.72PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X2 [Cucum... [more]
gi|596153974|ref|XP_007222797.1|8.1e-19863.62hypothetical protein PRUPE_ppa003578mg [Prunus persica][more]
gi|645224820|ref|XP_008219290.1|1.2e-19663.27PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR006501Pectinesterase_inhib_dom
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005618 cell wall
cellular_component GO:0005575 cellular_component
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU113396cucumber EST collection version 3.0transcribed_cluster
CU142604cucumber EST collection version 3.0transcribed_cluster
CU172114cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa3G890080.1Csa3G890080.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU113396CU113396transcribed_cluster
CU172114CU172114transcribed_cluster
CU142604CU142604transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 251..550
score: 3.5E
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 38..188
score: 3.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 38..162
score: 6.9
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 30..186
score: 1.0
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 12..160
score: 1.4
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 38..189
score: 1.83
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 247..562
score: 6.28E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 248..561
score: 9.8E
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 1..565
score:
NoneNo IPR availablePANTHERPTHR31707:SF38PECTINESTERASE/PECTINESTERASE INHIBITOR 20-RELATEDcoord: 1..565
score:

The following gene(s) are paralogous to this gene:

None