Csa3G819870 (gene) Cucumber (Chinese Long) v2

NameCsa3G819870
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionWerner Syndrome-like exonuclease; contains IPR012337 (Ribonuclease H-like domain)
LocationChr3 : 31850063 .. 31850981 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCCTTTTACCTGCTTTAGTCTTTGAAGTTCTCAGCAATGGCGATCACCATTGTTGACCATGAAGTTCCCTCAGATTCCCACAATTACTTCGACATAACCTTCGACACTGACGAGCCAATTCTCACTCTTCTCACCACTTCACCATCCATGGTAGACGGTTGGATTTCACAAACCCTCGCCATTCAAACTCCACCTCTCCTCGTCGGCCTCGACATCGAATGGCGCCCTAACAATCGCTCCTACGACAATCCTGTTGCCACCTTACAACTCTGCATCGGCCGCCGCTGCCTGATCTTGCAATTGCTTCACATGCCAGAGATCCCTAAATCTCTGTTCGAGTTTTTGGAAAACGAATCATTCACATTCGTAGGAGTGGGAATCGACGAAGATGCGGAAAAGCTAAACTGTGACTACGGATTGAAAGTGGGGAAGAGAATGGATCTGAGGGATTTGGCTGAGAGTGTAACGGGAAGAGGAGAATTGAAAAATGCGGGGTTGAAGAGATTGGGGAAAGAAGTGTTGGGGAAAGAGATTCATAAACCGAAGAGTGTGACGATGAGTAGATGGGATCAAGAATGGCTTACGCTTAATCAGGTGAAGTATGCTTGTATTGATGCGTTTTTCTCGTTTGAGATTGGAAAGTTTTTGCAATCTGCATCCTATTGAAAGGGGAATTTGGGGATTTTTCTTTCTTCTTTTTTTAAGGTTTAAGGGTTATTTGTTTGTTTTGGCTGCTTGATGGATGTGTTCTTAGCTTGGCTATGGTGTTTTTCTTTGCAAATCAAAACTTCGTGGAAATTTGGATGGATTGGATGATAATTTGCTCTTTACTTTACCAAACTTGGATCTCAATTTTAGAATTTACTGTGGTGGAGATCAAATGGTTTGTGATTAGTATGTAAACTATATTGAACATG

mRNA sequence

ATGGCGATCACCATTGTTGACCATGAAGTTCCCTCAGATTCCCACAATTACTTCGACATAACCTTCGACACTGACGAGCCAATTCTCACTCTTCTCACCACTTCACCATCCATGGTAGACGGTTGGATTTCACAAACCCTCGCCATTCAAACTCCACCTCTCCTCGTCGGCCTCGACATCGAATGGCGCCCTAACAATCGCTCCTACGACAATCCTGTTGCCACCTTACAACTCTGCATCGGCCGCCGCTGCCTGATCTTGCAATTGCTTCACATGCCAGAGATCCCTAAATCTCTGTTCGAGTTTTTGGAAAACGAATCATTCACATTCGTAGGAGTGGGAATCGACGAAGATGCGGAAAAGCTAAACTGTGACTACGGATTGAAAGTGGGGAAGAGAATGGATCTGAGGGATTTGGCTGAGAGTGTAACGGGAAGAGGAGAATTGAAAAATGCGGGGTTGAAGAGATTGGGGAAAGAAGTGTTGGGGAAAGAGATTCATAAACCGAAGAGTGTGACGATGAGTAGATGGGATCAAGAATGGCTTACGCTTAATCAGGTGAAGTATGCTTGTATTGATGCGTTTTTCTCGTTTGAGATTGGAAAGTTTTTGCAATCTGCATCCTATTGA

Coding sequence (CDS)

ATGGCGATCACCATTGTTGACCATGAAGTTCCCTCAGATTCCCACAATTACTTCGACATAACCTTCGACACTGACGAGCCAATTCTCACTCTTCTCACCACTTCACCATCCATGGTAGACGGTTGGATTTCACAAACCCTCGCCATTCAAACTCCACCTCTCCTCGTCGGCCTCGACATCGAATGGCGCCCTAACAATCGCTCCTACGACAATCCTGTTGCCACCTTACAACTCTGCATCGGCCGCCGCTGCCTGATCTTGCAATTGCTTCACATGCCAGAGATCCCTAAATCTCTGTTCGAGTTTTTGGAAAACGAATCATTCACATTCGTAGGAGTGGGAATCGACGAAGATGCGGAAAAGCTAAACTGTGACTACGGATTGAAAGTGGGGAAGAGAATGGATCTGAGGGATTTGGCTGAGAGTGTAACGGGAAGAGGAGAATTGAAAAATGCGGGGTTGAAGAGATTGGGGAAAGAAGTGTTGGGGAAAGAGATTCATAAACCGAAGAGTGTGACGATGAGTAGATGGGATCAAGAATGGCTTACGCTTAATCAGGTGAAGTATGCTTGTATTGATGCGTTTTTCTCGTTTGAGATTGGAAAGTTTTTGCAATCTGCATCCTATTGA

Protein sequence

MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDIEWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQSASY*
BLAST of Csa3G819870 vs. Swiss-Prot
Match: WEX_ARATH (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.6e-18
Identity = 59/153 (38.56%), Postives = 87/153 (56.86%), Query Frame = 1

Query: 56  VGLDIEWRPNNRSYDNP--VATLQLCIGRR-CLILQLLHMPEIPKSLFEFLENESFTFVG 115
           VGLDIEWRP+ R    P  VAT+Q+C+    C ++ + H   IP+SL   +E+ +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSG-IPQSLQHLIEDSTLVKVG 189

Query: 116 VGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSV 175
           +GID D+ KL  DYG+ +    DL DLA    G G+ K  GL  L + ++ KE+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIG-GD-KKWGLASLTETLVCKELLKPNRI 249

Query: 176 TMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQ 206
            +  W+   L+  Q++YA  DA+ S+ + K L+
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

BLAST of Csa3G819870 vs. Swiss-Prot
Match: WRN_HUMAN (Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 2.4e-14
Identity = 54/153 (35.29%), Postives = 79/153 (51.63%), Query Frame = 1

Query: 55  LVGLDIEWRP-NNRSYDNPVATLQLCIGR-RCLILQLLHMPEIPKSLFEFLENESFTFVG 114
           +VG D+EW P  NR     VA +QLC+   +C +  +  M   P+ L   LEN++    G
Sbjct: 78  VVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAG 137

Query: 115 VGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSV 174
           VGI+ D  KL  D+ +K+   ++L D+A       E  +  L  L K +LGK++ K KS+
Sbjct: 138 VGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWS--LNSLVKHLLGKQLLKDKSI 197

Query: 175 TMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQ 206
             S W +  LT +Q  YA  DA+  F I + L+
Sbjct: 198 RCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLE 228

BLAST of Csa3G819870 vs. Swiss-Prot
Match: WRN_MOUSE (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus GN=Wrn PE=1 SV=3)

HSP 1 Score: 77.8 bits (190), Expect = 1.6e-13
Identity = 53/146 (36.30%), Postives = 74/146 (50.68%), Query Frame = 1

Query: 55  LVGLDIEW----RPNNRSYDNPVATLQLCIGR-RCLILQLLHMPEIPKSLFEFLENESFT 114
           +VG D+EW    +P  RS    VA +QLC+   +C +  +  M   P+ L   LEN+S  
Sbjct: 72  VVGFDMEWPPIYKPGKRSR---VAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIK 131

Query: 115 FVGVGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKP 174
             GVGI+ D  KL  D+ +K+   ++L D+A       E  +  L  L K VLGK++ K 
Sbjct: 132 KAGVGIEGDQWKLLRDFDVKLESFVELTDVANEKLKCAETWS--LNGLVKHVLGKQLLKD 191

Query: 175 KSVTMSRWDQEWLTLNQVKYACIDAF 196
           KS+  S W    LT +Q  YA  DA+
Sbjct: 192 KSIRCSNWSNFPLTEDQKLYAATDAY 212

BLAST of Csa3G819870 vs. Swiss-Prot
Match: WRN_XENLA (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.0e-13
Identity = 55/155 (35.48%), Postives = 77/155 (49.68%), Query Frame = 1

Query: 55  LVGLDIEWRP-NNRSYDNPVATLQLCIG-RRCLILQLLHMPEIPKSLFEFLENESFTFVG 114
           ++G DIEW P   +     VA +Q+C+  ++C +  +  M   PK L   LE+ES   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 115 VGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGE--LKNAGLKRLGKEVLGKEIHKPK 174
           VGI+ D  KL  DY LK+   ++L ++A       E    N  +K L KE    +++K K
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKLRCKEKWTFNGLIKHLFKE----QLYKRK 191

Query: 175 SVTMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQ 206
           S   S WD   LT +Q  YA  DA+    I K L+
Sbjct: 192 SYRCSNWDIFLLTEDQKLYAATDAYAGLLIYKKLE 222

BLAST of Csa3G819870 vs. Swiss-Prot
Match: EXD2_HUMAN (Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens GN=EXD2 PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 4.3e-11
Identity = 54/160 (33.75%), Postives = 76/160 (47.50%), Query Frame = 1

Query: 55  LVGLDIEWRPNNRSYDNPVATLQLCI-GRRCLILQLLHM----PEIPKSLFEFLENESFT 114
           ++G+D EW  N     +P++ LQ+      C++++L  +      +P++L + L + +  
Sbjct: 104 VLGIDCEW-VNLEGKASPLSLLQMASPSGLCVLVRLPKLICGGKTLPRTLLDILADGTIL 163

Query: 115 FVGVGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKP 174
            VGVG  EDA KL  DYGL V   +DLR LA             LK L + VL   + K 
Sbjct: 164 KVGVGCSEDASKLLQDYGLVVRGCLDLRYLAMRQRNNLLCNGLSLKSLAETVLNFPLDKS 223

Query: 175 KSVTMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQSASY 210
             +  S WD E LT +QV YA  DA  S  +  FL    Y
Sbjct: 224 LLLRCSNWDAETLTEDQVIYAARDAQIS--VALFLHLLGY 260

BLAST of Csa3G819870 vs. TrEMBL
Match: A0A0A0LBN3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819870 PE=4 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 9.0e-117
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 1

Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSASY 210
           WLTLNQVKYACIDAFFSFEIGKFLQSASY
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQSASY 209

BLAST of Csa3G819870 vs. TrEMBL
Match: E5GBP0_CUCME (3'-5' exonuclease OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 2.3e-112
Identity = 201/208 (96.63%), Postives = 204/208 (98.08%), Query Frame = 1

Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           M+ITIVDHEVPSDSHNYFDITFD DEPILTLLTTSPSMVD WISQTL IQTPPLLVGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQLLH+PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 209
           WLTLNQVKYACIDAFFSFEIG+FLQS S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSES 208

BLAST of Csa3G819870 vs. TrEMBL
Match: A0A0A0LHC5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819830 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 4.1e-61
Identity = 122/208 (58.65%), Postives = 156/208 (75.00%), Query Frame = 1

Query: 3   ITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAI---QTPPLLVGLD 62
           +TI D ++P +SH+Y+DITFD DE ILTL+T SPSMVD WIS+ L I   +   L+VGLD
Sbjct: 4   LTISDRQIPLNSHDYYDITFDDDE-ILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLD 63

Query: 63  IEWRPN-NRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDED 122
           +EWRPN +R + NPVATLQLC+GRRCLI QL+H  E P+SL +FLE+++FTFVGVGID D
Sbjct: 64  VEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDND 123

Query: 123 AEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWD 182
             KL  DY L V   +DLR+LA       E + AGLK LG+EVLG+EI KP++V +SRWD
Sbjct: 124 VLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWD 183

Query: 183 QEWLTLNQVKYACIDAFFSFEIGKFLQS 207
           ++WL   Q+ YA +DAF SFEIG++L S
Sbjct: 184 RQWLNPAQILYATVDAFLSFEIGRYLLS 210

BLAST of Csa3G819870 vs. TrEMBL
Match: B9SNW3_RICCO (3-5 exonuclease, putative OS=Ricinus communis GN=RCOM_0582440 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.6e-60
Identity = 124/207 (59.90%), Postives = 154/207 (74.40%), Query Frame = 1

Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAI--QTPPLLVGL 60
           M + I D+E+ +D+HN +DITF TD+ I TL+T SPS+V+ W+ +T     QT P +VGL
Sbjct: 1   MTLGIRDYELINDTHNLYDITFFTDQ-IHTLVTHSPSLVEQWLIETQGQNNQTQPTIVGL 60

Query: 61  DIEWRPN-NRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDE 120
           D+EWRPN +R  +NPVATLQLCIG RCLI QL+H P IP+SLF+FL+N S+ F GVGI+ 
Sbjct: 61  DVEWRPNFSRHIENPVATLQLCIGSRCLIYQLIHSPRIPQSLFDFLKNSSYVFAGVGIES 120

Query: 121 DAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRW 180
           D EKL  DYGL VG  M+LR +A    G  ELKNAGLK L K+VLGKEI KPK VTMSRW
Sbjct: 121 DVEKLVEDYGLSVGNVMELRRVAAESLGVKELKNAGLKELVKQVLGKEIQKPKRVTMSRW 180

Query: 181 DQEWLTLNQVKYACIDAFFSFEIGKFL 205
           D  WL+ +QV+YAC+DAF   EIG+ L
Sbjct: 181 DSMWLSHDQVQYACLDAFVCSEIGRIL 206

BLAST of Csa3G819870 vs. TrEMBL
Match: A0A059A3P5_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K01677 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.5e-58
Identity = 123/208 (59.13%), Postives = 143/208 (68.75%), Query Frame = 1

Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           M I IVDHE+P D+HN +D+TF  D  + TLLT+SP M   W+S      +  LLVGLD+
Sbjct: 1   MTIGIVDHELPDDTHNLYDVTF-YDLSVRTLLTSSPPMAASWLSSR---GSQALLVGLDV 60

Query: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDA 120
           EWRPN  R  DNPVA LQL +G  CLI QLLH PEIP +L +FL +    FVGVGIDED 
Sbjct: 61  EWRPNFTRDSDNPVAILQLSVGLHCLIFQLLHAPEIPPTLADFLRDPRHVFVGVGIDEDV 120

Query: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180
           EKL CDYGL VG  +DLR LA    GR EL  AGLK L KEVLG+EI KPK V+ SRWD 
Sbjct: 121 EKLLCDYGLNVGNTVDLRALAARELGREELGRAGLKTLAKEVLGREIEKPKRVSKSRWDN 180

Query: 181 EWLTLNQVKYACIDAFFSFEIGKFLQSA 208
            WLT  QV+YAC+DAF S  IG+ L +A
Sbjct: 181 PWLTSVQVQYACLDAFVSLRIGEALNAA 204

BLAST of Csa3G819870 vs. TAIR10
Match: AT3G12410.1 (AT3G12410.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 101.3 bits (251), Expect = 7.5e-22
Identity = 68/212 (32.08%), Postives = 110/212 (51.89%), Query Frame = 1

Query: 13  DSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTL---AIQTPPLLVGLDIEW------- 72
           ++H  + + F  DE I+T+ T   S++  WI   L      + PL+VG+ ++W       
Sbjct: 13  NTHLEYSVDFFGDEFIVTV-TWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYS 72

Query: 73  --RPNN--------RSY-DNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFV 132
             RPNN        R Y DNP   LQLC+G RCLI+QL +  ++P +L  FL +   TFV
Sbjct: 73  DPRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFV 132

Query: 133 GVGIDEDAEKL-NCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKE-IHKP 192
           GV   +DA KL  C + L++G+ +D+R       GR  ++ +  + + +E +G + +   
Sbjct: 133 GVWNGQDAGKLARCCHQLEIGELLDIRRYVTDSWGR-SMRRSSFEEIVEECMGYQGVMLD 192

Query: 193 KSVTMSRWDQEWLTLNQVKYACIDAFFSFEIG 202
             ++MS W    L L+Q+  A +DA+   ++G
Sbjct: 193 PEISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

BLAST of Csa3G819870 vs. TAIR10
Match: AT2G36110.1 (AT2G36110.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 97.1 bits (240), Expect = 1.4e-20
Identity = 61/182 (33.52%), Postives = 94/182 (51.65%), Query Frame = 1

Query: 26  EPILTLLTTSPSMVDGWISQTLAIQ----TPPLLVGLDIEWRPNNRSYDNPVATLQLCIG 85
           E ++  +T + S +  WI           + PL+VGLD++W P     D P   LQLC+G
Sbjct: 24  ERLIVTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTPGGS--DPPPDILQLCVG 83

Query: 86  RRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAEKL-NCDYGLKVGKRMDLRDLA 145
            RCLI+QL H   IP+ L  FLE+E+ TFVGV   +D  KL    + L++ + +D+R   
Sbjct: 84  NRCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLEIWRLLDIRHYL 143

Query: 146 ESVTGRGELKNAGLKRLGKEVLG-KEIHKPKSVTMSRWDQEWLTLNQVKYACIDAFFSFE 202
            +      L N+  +++ +E LG K + K K + MS W    L+ +Q+  A  D +   +
Sbjct: 144 PT-----RLLNSSFEKIVEECLGYKGVRKDKEICMSNWGARSLSHDQIVQASDDVYVCCK 198

BLAST of Csa3G819870 vs. TAIR10
Match: AT3G12470.1 (AT3G12470.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 94.7 bits (234), Expect = 7.0e-20
Identity = 62/193 (32.12%), Postives = 101/193 (52.33%), Query Frame = 1

Query: 14  SHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQ----TPPLLVGLDIEWRPNNRSY 73
           +H    + F  D+ I+T+  T+ S++  WI    +        PL+VG+ ++WRP++   
Sbjct: 13  THQKHFVDFFGDDLIVTVTPTA-SVIRRWIRSVRSYNRNHSVHPLVVGIGVQWRPDSSDP 72

Query: 74  DNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAEKL-NCDYGL 133
           D P  TLQLC+G RCLI+QL +   +PK L  FL +   TFVGV   +D +KL  C + +
Sbjct: 73  DPPPKTLQLCVGSRCLIIQLGYNYGLPKVLRTFLADPKTTFVGVWNGQDQKKLEKCRHRV 132

Query: 134 KVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGK-EIHKPKSVTMSRWDQEWLTLNQV 193
           ++GK +D+R       G   +     +++ KE LG+  +    ++ MS W    L   QV
Sbjct: 133 EIGKLLDIRMFVRDSRG-ARMCFCSFEQIVKERLGRVGVRLDPAICMSDWGVYNLNHYQV 192

Query: 194 KYACIDAFFSFEI 201
             A I+++  F++
Sbjct: 193 LQASIESYVCFKL 203

BLAST of Csa3G819870 vs. TAIR10
Match: AT4G13870.2 (AT4G13870.2 Werner syndrome-like exonuclease)

HSP 1 Score: 93.2 bits (230), Expect = 2.0e-19
Identity = 59/153 (38.56%), Postives = 87/153 (56.86%), Query Frame = 1

Query: 56  VGLDIEWRPNNRSYDNP--VATLQLCIGRR-CLILQLLHMPEIPKSLFEFLENESFTFVG 115
           VGLDIEWRP+ R    P  VAT+Q+C+    C ++ + H   IP+SL   +E+ +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSG-IPQSLQHLIEDSTLVKVG 189

Query: 116 VGIDEDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSV 175
           +GID D+ KL  DYG+ +    DL DLA    G G+ K  GL  L + ++ KE+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIG-GD-KKWGLASLTETLVCKELLKPNRI 249

Query: 176 TMSRWDQEWLTLNQVKYACIDAFFSFEIGKFLQ 206
            +  W+   L+  Q++YA  DA+ S+ + K L+
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

BLAST of Csa3G819870 vs. TAIR10
Match: AT3G12430.1 (AT3G12430.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 91.7 bits (226), Expect = 5.9e-19
Identity = 58/202 (28.71%), Postives = 94/202 (46.53%), Query Frame = 1

Query: 26  EPILTLLTTSPSMVDGWISQTLA---IQTPPLLVGLDIEWRPNN---------------- 85
           E  + ++T  PS++  WI   L+     + PL+VG+ ++W P++                
Sbjct: 24  EEFIVIVTPDPSVIGQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRYRSDSPP 83

Query: 86  -----------RSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGI 145
                      R +D P  TLQLC+G RC+I+QL H   +P+ L  FL +  +TFVG+  
Sbjct: 84  VRYRSDSPRVRRRFDPPADTLQLCVGNRCIIIQLSHCERVPQVLRNFLADRDYTFVGIWN 143

Query: 146 DEDAEKL-NCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKE-IHKPKSVT 196
            +DA KL    + L++   +DLR      +GR  +K    +++ +E LG   +   + V+
Sbjct: 144 SQDAGKLKRSKHELEIAVLLDLRKFVSDSSGR-TMKRCSFEKIVEENLGHSGVRLDRKVS 203

BLAST of Csa3G819870 vs. NCBI nr
Match: gi|778685161|ref|XP_011652174.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 427.6 bits (1098), Expect = 1.3e-116
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 1

Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSASY 210
           WLTLNQVKYACIDAFFSFEIGKFLQSASY
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQSASY 209

BLAST of Csa3G819870 vs. NCBI nr
Match: gi|659085101|ref|XP_008443244.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo])

HSP 1 Score: 412.9 bits (1060), Expect = 3.3e-112
Identity = 201/208 (96.63%), Postives = 204/208 (98.08%), Query Frame = 1

Query: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60
           M+ITIVDHEVPSDSHNYFDITFD DEPILTLLTTSPSMVD WISQTL IQTPPLLVGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120
           EWRPNNRSYDNPVATLQLCIGRRCLILQLLH+PEIPKSLFEFLENESFTFVGVGIDEDAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180
           KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 209
           WLTLNQVKYACIDAFFSFEIG+FLQS S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSES 208

BLAST of Csa3G819870 vs. NCBI nr
Match: gi|1021483340|ref|XP_016184712.1| (PREDICTED: Werner Syndrome-like exonuclease [Arachis ipaensis])

HSP 1 Score: 244.6 bits (623), Expect = 1.6e-61
Identity = 122/208 (58.65%), Postives = 152/208 (73.08%), Query Frame = 1

Query: 3   ITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTL-----AIQTPPLLVG 62
           I++VDH +P D +N +D+ F T   I TLLTTSPS+VD W+S TL     +  T  +LVG
Sbjct: 24  ISVVDHNLPYDDYNLYDVIFHT-HTIHTLLTTSPSLVDTWLSDTLNHHSSSTTTTTVLVG 83

Query: 63  LDIEWRPN-NRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGID 122
           LDIEWRPN +R+ +NPVATLQLCIG RCL+ Q+LH P +P+SL +FL NE  TF+GVG++
Sbjct: 84  LDIEWRPNAHRNSNNPVATLQLCIGNRCLVFQILHSPSVPQSLVDFLVNEGHTFLGVGVE 143

Query: 123 EDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSR 182
           ED EKL  DY L V   +DLRDLAE+V G  E+K AGLK L + VLG EI KPK ++ SR
Sbjct: 144 EDVEKLLEDYNLNVKNFVDLRDLAENVLGESEMKRAGLKSLAERVLGLEIEKPKRISRSR 203

Query: 183 WDQEWLTLNQVKYACIDAFFSFEIGKFL 205
           WD  WLT  QV+YACIDAF SFE+G+ L
Sbjct: 204 WDNAWLTAEQVQYACIDAFVSFEVGRML 230

BLAST of Csa3G819870 vs. NCBI nr
Match: gi|1012114599|ref|XP_015960836.1| (PREDICTED: Werner Syndrome-like exonuclease [Arachis duranensis])

HSP 1 Score: 243.8 bits (621), Expect = 2.7e-61
Identity = 124/208 (59.62%), Postives = 150/208 (72.12%), Query Frame = 1

Query: 3   ITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTL-----AIQTPPLLVG 62
           I++VDH +P D +N +D+ F T   I TLLTTSPS+VD W+S TL     +  T  +LVG
Sbjct: 24  ISVVDHNLPYDDYNLYDVIFHT-HTIHTLLTTSPSLVDTWLSDTLNHYHSSTTTTTVLVG 83

Query: 63  LDIEWRPN-NRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGID 122
           LDIEWRPN +R+ DNPVATLQLCIG  CLI Q+LH P +P+SL +FL NE  TF+GVGI+
Sbjct: 84  LDIEWRPNTHRNSDNPVATLQLCIGNSCLIFQILHSPSVPRSLVDFLVNEGHTFLGVGIE 143

Query: 123 EDAEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSR 182
           ED EKL  DY L V   +DLR LAESV G  E+K AGLKRL + VLG EI KPK V+ SR
Sbjct: 144 EDVEKLLEDYNLNVKNFVDLRGLAESVLGESEMKRAGLKRLAERVLGLEIEKPKRVSRSR 203

Query: 183 WDQEWLTLNQVKYACIDAFFSFEIGKFL 205
           WD  WLT  QV+YAC+DAF S E+G+ L
Sbjct: 204 WDNAWLTAEQVQYACVDAFVSLEVGRML 230

BLAST of Csa3G819870 vs. NCBI nr
Match: gi|449437986|ref|XP_004136771.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 242.7 bits (618), Expect = 5.9e-61
Identity = 122/208 (58.65%), Postives = 156/208 (75.00%), Query Frame = 1

Query: 3   ITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAI---QTPPLLVGLD 62
           +TI D ++P +SH+Y+DITFD DE ILTL+T SPSMVD WIS+ L I   +   L+VGLD
Sbjct: 4   LTISDRQIPLNSHDYYDITFDDDE-ILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLD 63

Query: 63  IEWRPN-NRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDED 122
           +EWRPN +R + NPVATLQLC+GRRCLI QL+H  E P+SL +FLE+++FTFVGVGID D
Sbjct: 64  VEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDND 123

Query: 123 AEKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWD 182
             KL  DY L V   +DLR+LA       E + AGLK LG+EVLG+EI KP++V +SRWD
Sbjct: 124 VLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWD 183

Query: 183 QEWLTLNQVKYACIDAFFSFEIGKFLQS 207
           ++WL   Q+ YA +DAF SFEIG++L S
Sbjct: 184 RQWLNPAQILYATVDAFLSFEIGRYLLS 210

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WEX_ARATH3.6e-1838.56Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1[more]
WRN_HUMAN2.4e-1435.29Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2[more]
WRN_MOUSE1.6e-1336.30Werner syndrome ATP-dependent helicase homolog OS=Mus musculus GN=Wrn PE=1 SV=3[more]
WRN_XENLA6.0e-1335.48Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=... [more]
EXD2_HUMAN4.3e-1133.75Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens GN=EXD2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LBN3_CUCSA9.0e-117100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819870 PE=4 SV=1[more]
E5GBP0_CUCME2.3e-11296.633'-5' exonuclease OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0LHC5_CUCSA4.1e-6158.65Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819830 PE=4 SV=1[more]
B9SNW3_RICCO1.6e-6059.903-5 exonuclease, putative OS=Ricinus communis GN=RCOM_0582440 PE=4 SV=1[more]
A0A059A3P5_EUCGR1.5e-5859.13Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K01677 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G12410.17.5e-2232.08 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT2G36110.11.4e-2033.52 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT3G12470.17.0e-2032.12 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT4G13870.22.0e-1938.56 Werner syndrome-like exonuclease[more]
AT3G12430.15.9e-1928.71 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778685161|ref|XP_011652174.1|1.3e-116100.00PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus][more]
gi|659085101|ref|XP_008443244.1|3.3e-11296.63PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo][more]
gi|1021483340|ref|XP_016184712.1|1.6e-6158.65PREDICTED: Werner Syndrome-like exonuclease [Arachis ipaensis][more]
gi|1012114599|ref|XP_015960836.1|2.7e-6159.62PREDICTED: Werner Syndrome-like exonuclease [Arachis duranensis][more]
gi|449437986|ref|XP_004136771.1|5.9e-6158.65PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR0025623'-5'_exonuclease_dom
IPR012337RNaseH-like_sf
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:00084083'-5' exonuclease activity
Vocabulary: Biological Process
TermDefinition
GO:0006139nucleobase-containing compound metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006139 nucleobase-containing compound metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU117067cucumber EST collection version 3.0transcribed_cluster
CU162643cucumber EST collection version 3.0transcribed_cluster
CU173024cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa3G819870.1Csa3G819870.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU162643CU162643transcribed_cluster
CU173024CU173024transcribed_cluster
CU117067CU117067transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0025623'-5' exonuclease domainPFAMPF01612DNA_pol_A_exo1coord: 42..206
score: 1.9
IPR0025623'-5' exonuclease domainSMARTSM0047435exoneu6coord: 36..209
score: 2.
IPR012337Ribonuclease H-like domainGENE3DG3DSA:3.30.420.10coord: 14..205
score: 1.9
IPR012337Ribonuclease H-like domainunknownSSF53098Ribonuclease H-likecoord: 51..205
score: 2.47
NoneNo IPR availablePANTHERPTHR136203-5 EXONUCLEASEcoord: 1..209
score: 8.1