Csa3G285030 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa3G285030
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Valine--tRNA ligase; contains IPR002303 (Valine-tRNA ligase), IPR010978 (tRNA-binding arm)
Location	Chr3 : 16800588 .. 16801987 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAATAAATCTCGTAAAATTCAATCTTCTAAATCAACCCATGCAGAATTTCTGAATCTTCAAATTGTGGTAGCCCTATTTCATATCTCATTGCTGACTCTATTTTATCATACCTAGTGGCACAACTGATGTAAATCTCTTGCGGAAGTCATAAAACAATTATAACTTGTTTTTACTGCCTGCTGCAGGAAGAGAAGGAAGTTTTAGCTCTTCTTACTCGACTGGATCTACATAATGTCCATTTTGCAAATTCTCCTCCAGGCATGTCTATTACTTGAGTTATTTTTCTCATTTTTGTACCAGTATCTATTCGCATGCATATTTGTGGGTGATGGTTTCCGTGCCTTGTTAAATCATTTACCTAAGTTGGGTTAGACTGTTGAAGTATTATTTTTGCTTTATGTTTTTTTCCGCAGGAAATGTAGATCAGTCGGTGCACCTCGTTGCTGGTGAAGGGCTAGAGGCTTATCTACCTCTTGCTGATATGGTTGATATTTCTGCCGAAGTTCAACGCTTATCGAAGCGCCTTACAAAAATGAAAATAGAGTATGATGGATTTATAGCTCGGCTCAGTTCTCCAAGTGTACGTTACTTGCTCAGAAACATATTTCAGATTAGTATCTATTTACATGTTTTTTGCCTGAAATAGAATACATATGAAATAGGTAACTCTACATAATATTGAAGTTTTCAGTTAGTAAAAGATAAACTAGTACAGAATTGTTTCTTCCTTGTATCTGCCTCGTCATTTCTTAATGATTCCGGGCTGCTTTTTTTTTTTTTTTTTGGGGGGGGGGGGGGGGAGGGGTTTGGGAACTTTGACTCATTTTTCAGTCACGTCCTCATACTTATCCAACAACATCAAGTCAAATCTTACAAATTGAAAAGTGATCATTGTGAACAACGTGGCAAGGGAGGTCCAAATCAATTTGACTTTGTATAGTTGAATTTAGTATTGCATTTATATATCAGTGCTTTTCTTACAGCCACTTTTTAGTGACTCACACCCGGATGAACACCCTGTTTTTCTGTTTATTTGAAATGAGGGACTGATATAGCAAATACATTTTATTTTACATTTGATATTCATTAGTCAATGCGTTGGATATGTAAGCTTCTGATTTCAACCTAAAATGATGTTACATTTTAATCTATTCACCAGAACAAGACTTCAGCTTTTTTCCTTTAAGAAATACATAAACTGAAAGGGATTTTAATCACACCAAAACATATTATAGCTCTTGCGGTCTGGTTTGATCTTGCAGTTTGTAGAGAAAGCTCCCGAGGACATCGTCCGTGGTGTAAGAGAAAAGGCTGAAGAAGCGAAAGAGAAGATAGCTCTCACCGAGAAGCGCCTTTCTCTCCTTGGATCAACTGTTCCAGTTCCTGATTGTTGA

mRNA sequence

ATGGAAAATAAATCTCGTAAAATTCAATCTTCTAAATCAACCCATGCAGAATTTCTGAATCTTCAAATTGTGGAAGAGAAGGAAGTTTTAGCTCTTCTTACTCGACTGGATCTACATAATGTCCATTTTGCAAATTCTCCTCCAGGAAATGTAGATCAGTCGGTGCACCTCGTTGCTGGTGAAGGGCTAGAGGCTTATCTACCTCTTGCTGATATGGTTGATATTTCTGCCGAAGTTCAACGCTTATCGAAGCGCCTTACAAAAATGAAAATAGAGTATGATGGATTTATAGCTCGGCTCAGTTCTCCAAGTTTTGTAGAGAAAGCTCCCGAGGACATCGTCCGTGGTGTAAGAGAAAAGGCTGAAGAAGCGAAAGAGAAGATAGCTCTCACCGAGAAGCGCCTTTCTCTCCTTGGATCAACTGTTCCAGTTCCTGATTGTTGA

Coding sequence (CDS)

ATGGAAAATAAATCTCGTAAAATTCAATCTTCTAAATCAACCCATGCAGAATTTCTGAATCTTCAAATTGTGGAAGAGAAGGAAGTTTTAGCTCTTCTTACTCGACTGGATCTACATAATGTCCATTTTGCAAATTCTCCTCCAGGAAATGTAGATCAGTCGGTGCACCTCGTTGCTGGTGAAGGGCTAGAGGCTTATCTACCTCTTGCTGATATGGTTGATATTTCTGCCGAAGTTCAACGCTTATCGAAGCGCCTTACAAAAATGAAAATAGAGTATGATGGATTTATAGCTCGGCTCAGTTCTCCAAGTTTTGTAGAGAAAGCTCCCGAGGACATCGTCCGTGGTGTAAGAGAAAAGGCTGAAGAAGCGAAAGAGAAGATAGCTCTCACCGAGAAGCGCCTTTCTCTCCTTGGATCAACTGTTCCAGTTCCTGATTGTTGA

Protein sequence

MENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLLGSTVPVPDC*

Homology

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BLAST of Csa3G285030 vs. Swiss-Prot
Match: SYVM2_ARATH (Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana GN=EMB2247 PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 1.6e-41
Identity = 89/136 (65.44%), Postives = 108/136 (79.41%), Query Frame = 1


Query: 6   RKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEA 65
           ++I +S    AE +   I +EKEVLALL+RLDL+NVHF+N+PPG+ + SVHLVA EGLEA
Sbjct: 834 KRISASVVGSAEVIEY-ISKEKEVLALLSRLDLNNVHFSNAPPGDANLSVHLVASEGLEA 893

Query: 66  YLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAK 125
           YLPLA MVDIS+EVQR+SKRL+KM+ EYD  I RLSSP FVEKAPE++VRGV+E+ EE +
Sbjct: 894 YLPLAAMVDISSEVQRISKRLSKMQTEYDALITRLSSPKFVEKAPEEVVRGVKEQVEELE 953

Query: 126 EKIALTEKRLSLLGST 142
           EKI LT+ RL  L ST
Sbjct: 954 EKIKLTKARLDFLKST 968

[Close]

BLAST of Csa3G285030 vs. Swiss-Prot
Match: SYV_FRATT (Valine--tRNA ligase OS=Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) GN=valS PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.0e-11
Identity = 41/102 (40.20%), Postives = 57/102 (55.88%), Query Frame = 1


Query: 38  LHNVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFI 97
           ++N+ F ++PP ++ Q V     EGLE  +PLA +VDI AE  RL K L K+K E D   
Sbjct: 823 INNIEFNDNPPTSLSQIV-----EGLELNIPLAGLVDIEAEKARLDKELDKLKDEVDRVQ 882

Query: 98  ARLSSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLLG 140
            +LS+  FV  APE +V   +EK  + +E  A T ++   LG
Sbjct: 883 KKLSNERFVSNAPEAVVAAEQEKLAKYQELYAKTLEKKEALG 919

[Close]

BLAST of Csa3G285030 vs. Swiss-Prot
Match: SYV_CLOAB (Valine--tRNA ligase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) GN=valS PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.6e-10
Identity = 35/99 (35.35%), Postives = 56/99 (56.57%), Query Frame = 1


Query: 40  NVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIAR 99
           +V F  S     + +V  V  +G E ++PL D++D++ E++RLSK   K+K E      +
Sbjct: 783 SVSFLKSKDEAPENAVSSVT-KGAELFMPLLDLIDVTKEIERLSKEKDKLKAEIQRVDKK 842

Query: 100 LSSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 139
           LS+  FV+KAPE +V   R K E+ K+ +   E+R++ L
Sbjct: 843 LSNKGFVDKAPESVVEAERVKGEKYKKMLEAVEERIAAL 880

[Close]

BLAST of Csa3G285030 vs. Swiss-Prot
Match: SYV_THEMA (Valine--tRNA ligase OS=Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) GN=valS PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.8e-09
Identity = 37/112 (33.04%), Postives = 58/112 (51.79%), Query Frame = 1


Query: 24  VEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAEVQRLS 83
           V E+E L L T  ++  V F N  P    ++      E +EAY+ L  ++D   E +RL 
Sbjct: 753 VTEEEELLLKTLGNIEEVSFVNEKP---PKTATAYVEEEIEAYVDLGGLIDFEKEKERLK 812

Query: 84  KRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRL 136
           + + K++ E D    +L++  FVEKAPE++V   +EK    +E++A  E  L
Sbjct: 813 QIMEKIQKEIDRLEKKLANKDFVEKAPEEVVEETKEKLNTNRERLARLESIL 861

[Close]

BLAST of Csa3G285030 vs. Swiss-Prot
Match: SYV_LEGPA (Valine--tRNA ligase OS=Legionella pneumophila (strain Paris) GN=valS PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.8e-09
Identity = 39/123 (31.71%), Postives = 69/123 (56.10%), Query Frame = 1


Query: 19  LNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAE 78
           L  +I + +++L  L+++D  N     +P   V  S   V GE +E  +P+AD++D  AE
Sbjct: 803 LKERIAKYEKILKTLSKIDKINYL---APDEKVPVSATAVLGE-IELLIPMADLIDKEAE 862

Query: 79  VQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREK---AEEAKEKIALTEKRL 138
           + RL+K L K+  + +    +L++P F +KAPE+I+   ++K   A+ AK+K+   + R+
Sbjct: 863 LSRLNKELAKLNKDIELAQGKLNNPKFTDKAPEEIIAKEKDKLAQAQVAKDKLLQHKNRI 921

[Close]

BLAST of Csa3G285030 vs. TrEMBL
Match: A0A0A0L9W5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G285030 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 5.1e-74
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 1


Query: 1   MENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAG 60
           MENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAG
Sbjct: 1   MENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAG 60

Query: 61  EGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREK 120
           EGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREK
Sbjct: 61  EGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREK 120

Query: 121 AEEAKEKIALTEKRLSLLGSTVPVPDC 148
           AEEAKEKIALTEKRLSLLGSTVPVPDC
Sbjct: 121 AEEAKEKIALTEKRLSLLGSTVPVPDC 147

[Close]

BLAST of Csa3G285030 vs. TrEMBL
Match: A0A151SWL9_CAJCA (Valyl-tRNA synthetase (Fragment) OS=Cajanus cajan GN=KK1_014623 PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 8.5e-45
Identity = 99/140 (70.71%), Postives = 119/140 (85.00%), Query Frame = 1


Query: 5   SRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLE 64
           +++I +S   + E +   I EE+EVLALL+RLDL N+HF +S PGN DQSVHLVAGEGLE
Sbjct: 802 AKRISASVVANNEIIEY-ISEEREVLALLSRLDLQNLHFTDSSPGNADQSVHLVAGEGLE 861

Query: 65  AYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEA 124
           AYLPLADMVDISAEV+RLSKRL+KM+ EYDGFIA+L+S  FVEKAPED+VRGVREKA EA
Sbjct: 862 AYLPLADMVDISAEVERLSKRLSKMQKEYDGFIAKLNSSQFVEKAPEDVVRGVREKAAEA 921

Query: 125 KEKIALTEKRLSLLGSTVPV 145
           +EKI LT+KRL+LL S+V V
Sbjct: 922 EEKINLTKKRLALLNSSVLV 940

[Close]

BLAST of Csa3G285030 vs. TrEMBL
Match: M5X3F2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002504mg PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 9.4e-44
Identity = 92/119 (77.31%), Postives = 106/119 (89.08%), Query Frame = 1


Query: 23  IVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAEVQRL 82
           IV+EKEVLALL+RLDL N+HF +SPPGN DQSVH+VAGEGLEAYLPLADM+DI+AE+QRL
Sbjct: 542 IVKEKEVLALLSRLDLQNIHFTDSPPGNADQSVHVVAGEGLEAYLPLADMIDITAEIQRL 601

Query: 83  SKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLLGST 142
            KRL+KM+ EYDG  ARLSSP FVEKAPEDIVRGV+EKA E +EKI LT+ RL+LL ST
Sbjct: 602 YKRLSKMQTEYDGLKARLSSPKFVEKAPEDIVRGVQEKAAETEEKITLTKNRLALLKST 660

[Close]

BLAST of Csa3G285030 vs. TrEMBL
Match: A0A0R0FQB9_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_17G186800 PE=4 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.6e-43
Identity = 98/140 (70.00%), Postives = 114/140 (81.43%), Query Frame = 1


Query: 5   SRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLE 64
           +++I +S   + E +   I EE+EVLALL+RLDL N+HF NS PGN DQSVHLVAGEGLE
Sbjct: 267 AKRISASVVANNEVIEY-IAEEREVLALLSRLDLQNLHFTNSFPGNADQSVHLVAGEGLE 326

Query: 65  AYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEA 124
           AYLPLADMVDISAEV RLSKRL+KM+ EYDG IA+L+SP FVEKAPE +VRGVREKA EA
Sbjct: 327 AYLPLADMVDISAEVDRLSKRLSKMQKEYDGLIAKLNSPQFVEKAPEAVVRGVREKATEA 386

Query: 125 KEKIALTEKRLSLLGSTVPV 145
           +EKI LT+KRL  L S V V
Sbjct: 387 EEKINLTKKRLEFLNSNVLV 405

[Close]

BLAST of Csa3G285030 vs. TrEMBL
Match: I1MW61_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=2)

HSP 1 Score: 183.7 bits (465), Expect = 1.6e-43
Identity = 98/140 (70.00%), Postives = 114/140 (81.43%), Query Frame = 1


Query: 5   SRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLE 64
           +++I +S   + E +   I EE+EVLALL+RLDL N+HF NS PGN DQSVHLVAGEGLE
Sbjct: 284 AKRISASVVANNEVIEY-IAEEREVLALLSRLDLQNLHFTNSFPGNADQSVHLVAGEGLE 343

Query: 65  AYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEA 124
           AYLPLADMVDISAEV RLSKRL+KM+ EYDG IA+L+SP FVEKAPE +VRGVREKA EA
Sbjct: 344 AYLPLADMVDISAEVDRLSKRLSKMQKEYDGLIAKLNSPQFVEKAPEAVVRGVREKATEA 403

Query: 125 KEKIALTEKRLSLLGSTVPV 145
           +EKI LT+KRL  L S V V
Sbjct: 404 EEKINLTKKRLEFLNSNVLV 422

[Close]

BLAST of Csa3G285030 vs. TAIR10
Match: AT5G16715.1 (AT5G16715.1 ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases)

HSP 1 Score: 170.2 bits (430), Expect = 9.3e-43
Identity = 89/136 (65.44%), Postives = 108/136 (79.41%), Query Frame = 1


Query: 6   RKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEA 65
           ++I +S    AE +   I +EKEVLALL+RLDL+NVHF+N+PPG+ + SVHLVA EGLEA
Sbjct: 834 KRISASVVGSAEVIEY-ISKEKEVLALLSRLDLNNVHFSNAPPGDANLSVHLVASEGLEA 893

Query: 66  YLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAK 125
           YLPLA MVDIS+EVQR+SKRL+KM+ EYD  I RLSSP FVEKAPE++VRGV+E+ EE +
Sbjct: 894 YLPLAAMVDISSEVQRISKRLSKMQTEYDALITRLSSPKFVEKAPEEVVRGVKEQVEELE 953

Query: 126 EKIALTEKRLSLLGST 142
           EKI LT+ RL  L ST
Sbjct: 954 EKIKLTKARLDFLKST 968

[Close]

BLAST of Csa3G285030 vs. NCBI nr
Match: gi|700202636|gb|KGN57769.1| (hypothetical protein Csa_3G285030 [Cucumis sativus])

HSP 1 Score: 285.0 bits (728), Expect = 7.3e-74
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 1


Query: 1   MENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAG 60
           MENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAG
Sbjct: 1   MENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAG 60

Query: 61  EGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREK 120
           EGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREK
Sbjct: 61  EGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREK 120

Query: 121 AEEAKEKIALTEKRLSLLGSTVPVPDC 148
           AEEAKEKIALTEKRLSLLGSTVPVPDC
Sbjct: 121 AEEAKEKIALTEKRLSLLGSTVPVPDC 147

[Close]

BLAST of Csa3G285030 vs. NCBI nr
Match: gi|778680743|ref|XP_011651389.1| (PREDICTED: valine--tRNA ligase isoform X3 [Cucumis sativus])

HSP 1 Score: 244.2 bits (622), Expect = 1.4e-61
Identity = 127/138 (92.03%), Postives = 130/138 (94.20%), Query Frame = 1


Query: 10  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 69
           S+    +E +N  I EEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 756 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 815

Query: 70  ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 129
           ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA
Sbjct: 816 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 875

Query: 130 LTEKRLSLLGSTVPVPDC 148
           LTEKRLSLLGSTVPVPDC
Sbjct: 876 LTEKRLSLLGSTVPVPDC 893

[Close]

BLAST of Csa3G285030 vs. NCBI nr
Match: gi|778680746|ref|XP_011651390.1| (PREDICTED: valine--tRNA ligase, mitochondrial isoform X4 [Cucumis sativus])

HSP 1 Score: 244.2 bits (622), Expect = 1.4e-61
Identity = 127/138 (92.03%), Postives = 130/138 (94.20%), Query Frame = 1


Query: 10  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 69
           S+    +E +N  I EEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 686 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 745

Query: 70  ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 129
           ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA
Sbjct: 746 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 805

Query: 130 LTEKRLSLLGSTVPVPDC 148
           LTEKRLSLLGSTVPVPDC
Sbjct: 806 LTEKRLSLLGSTVPVPDC 823

[Close]

BLAST of Csa3G285030 vs. NCBI nr
Match: gi|778680737|ref|XP_011651387.1| (PREDICTED: valine--tRNA ligase, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 244.2 bits (622), Expect = 1.4e-61
Identity = 127/138 (92.03%), Postives = 130/138 (94.20%), Query Frame = 1


Query: 10  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 69
           S+    +E +N  I EEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 832 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 891

Query: 70  ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 129
           ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA
Sbjct: 892 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 951

Query: 130 LTEKRLSLLGSTVPVPDC 148
           LTEKRLSLLGSTVPVPDC
Sbjct: 952 LTEKRLSLLGSTVPVPDC 969

[Close]

BLAST of Csa3G285030 vs. NCBI nr
Match: gi|778680740|ref|XP_011651388.1| (PREDICTED: probable valine--tRNA ligase, cytoplasmic isoform X2 [Cucumis sativus])

HSP 1 Score: 244.2 bits (622), Expect = 1.4e-61
Identity = 127/138 (92.03%), Postives = 130/138 (94.20%), Query Frame = 1


Query: 10  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 69
           S+    +E +N  I EEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 784 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 843

Query: 70  ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 129
           ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA
Sbjct: 844 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 903

Query: 130 LTEKRLSLLGSTVPVPDC 148
           LTEKRLSLLGSTVPVPDC
Sbjct: 904 LTEKRLSLLGSTVPVPDC 921

The following BLAST results are available for this feature:

BLAST of Csa3G285030 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SYVM2_ARATH	1.6e-41	65.44	Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana GN=EM...	[more]
SYV_FRATT	3.0e-11	40.20	Valine--tRNA ligase OS=Francisella tularensis subsp. tularensis (strain SCHU S4 ...	[more]
SYV_CLOAB	2.6e-10	35.35	Valine--tRNA ligase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / J...	[more]
SYV_THEMA	2.8e-09	33.04	Valine--tRNA ligase OS=Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 ...	[more]
SYV_LEGPA	4.8e-09	31.71	Valine--tRNA ligase OS=Legionella pneumophila (strain Paris) GN=valS PE=3 SV=1	[more]

BLAST of Csa3G285030 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9W5_CUCSA	5.1e-74	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G285030 PE=4 SV=1	[more]
A0A151SWL9_CAJCA	8.5e-45	70.71	Valyl-tRNA synthetase (Fragment) OS=Cajanus cajan GN=KK1_014623 PE=3 SV=1	[more]
M5X3F2_PRUPE	9.4e-44	77.31	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002504mg PE=4 SV=1	[more]
A0A0R0FQB9_SOYBN	1.6e-43	70.00	Uncharacterized protein OS=Glycine max GN=GLYMA_17G186800 PE=4 SV=1	[more]
I1MW61_SOYBN	1.6e-43	70.00	Uncharacterized protein OS=Glycine max PE=4 SV=2	[more]

BLAST of Csa3G285030 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16715.1	9.3e-43	65.44	ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide bi...	[more]

BLAST of Csa3G285030 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202636\|gb\|KGN57769.1\|	7.3e-74	100.00	hypothetical protein Csa_3G285030 [Cucumis sativus]	[more]
gi\|778680743\|ref\|XP_011651389.1\|	1.4e-61	92.03	PREDICTED: valine--tRNA ligase isoform X3 [Cucumis sativus]	[more]
gi\|778680746\|ref\|XP_011651390.1\|	1.4e-61	92.03	PREDICTED: valine--tRNA ligase, mitochondrial isoform X4 [Cucumis sativus]	[more]
gi\|778680737\|ref\|XP_011651387.1\|	1.4e-61	92.03	PREDICTED: valine--tRNA ligase, mitochondrial isoform X1 [Cucumis sativus]	[more]
gi\|778680740\|ref\|XP_011651388.1\|	1.4e-61	92.03	PREDICTED: probable valine--tRNA ligase, cytoplasmic isoform X2 [Cucumis sativus...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR010978	tRNA-bd_arm
IPR019499	Val-tRNA_synth_tRNA-bd

Vocabulary: **Molecular Function**
Term	Definition
GO:0000166	nucleotide binding
GO:0004832	valine-tRNA ligase activity
GO:0005524	ATP binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006438	valyl-tRNA aminoacylation

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009097	isoleucine biosynthetic process
biological_process	GO:0009098	leucine biosynthetic process
biological_process	GO:0006450	regulation of translational fidelity
biological_process	GO:0009099	valine biosynthetic process
biological_process	GO:0006438	valyl-tRNA aminoacylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0002161	aminoacyl-tRNA editing activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004832	valine-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa3G285030.1	Csa3G285030.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010978	tRNA-binding arm	unknown	SSF46589	tRNA-binding arm	coord: 74..138 score: 1.41
IPR019499	Valyl-tRNA synthetase, tRNA-binding arm	GENE3D	G3DSA:1.10.287.380		coord: 67..136 score: 1.
IPR019499	Valyl-tRNA synthetase, tRNA-binding arm	PFAM	PF10458	Val_tRNA-synt_C	coord: 74..138 score: 1.1
None	No IPR available	unknown	Coil	Coil	coord: 114..134 score: -coord: 72..92 scor
None	No IPR available	PANTHER	PTHR11946	ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES	coord: 22..46 score: 3.7E-23coord: 70..132 score: 3.7
None	No IPR available	PANTHER	PTHR11946:SF76	ATP BINDING/VALINE-TRNA LIGASE/AMINOACYL-TRNA LIGASE	coord: 70..132 score: 3.7E-23coord: 22..46 score: 3.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa3G285030	Cla013221	Watermelon (97103) v1	cuwmB239
Csa3G285030	MELO3C020199	Melon (DHL92) v3.5.1	cumeB246
Csa3G285030	ClCG05G011510	Watermelon (Charleston Gray)	cuwcgB226
Csa3G285030	Lsi05G003600	Bottle gourd (USVL1VR-Ls)	culsiB206
Csa3G285030	MELO3C020199.2	Melon (DHL92) v3.6.1	cumedB239
Csa3G285030	Cla97C05G093590	Watermelon (97103) v2	cuwmbB209
Csa3G285030	CSPI03G20870	Wild cucumber (PI 183967)	cpicuB120
Csa3G285030	Cucsa.031250	Cucumber (Gy14) v1	cgycuB003
Csa3G285030	CmoCh14G008350	Cucurbita moschata (Rifu)	cmocuB229
Csa3G285030	CsGy3G020460	Cucumber (Gy14) v2	cgybcuB109

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa3G285030	Cucurbita maxima (Rimu)	cmacuB242
Csa3G285030	Cucurbita maxima (Rimu)	cmacuB455
Csa3G285030	Cucurbita moschata (Rifu)	cmocuB442
Csa3G285030	Melon (DHL92) v3.5.1	cumeB161
Csa3G285030	Watermelon (Charleston Gray)	cuwcgB233
Csa3G285030	Watermelon (97103) v1	cuwmB245
Csa3G285030	Cucurbita pepo (Zucchini)	cpecuB547
Csa3G285030	Cucurbita pepo (Zucchini)	cpecuB600
Csa3G285030	Bottle gourd (USVL1VR-Ls)	culsiB212
Csa3G285030	Silver-seed gourd	carcuB0513
Csa3G285030	Silver-seed gourd	carcuB0780
Csa3G285030	Watermelon (97103) v2	cuwmbB218
Csa3G285030	Wax gourd	cuwgoB271
Csa3G285030	Wax gourd	cuwgoB300