Csa3G187220 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa3G187220
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Methyltransferase-like protein; contains IPR025714 (Methyltransferase domain), IPR026635 (N-lysine methyltransferase See1-like)
Location	Chr3 : 13128427 .. 13132908 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAAAGTTTTAAGAGAAAATTATCTTCCTTCATTGTAGTTGAGTTTTTTCTTCTAGAAAACAGGTTATGGTTTAGGCTCTTCTTTTCTACCATTTGGTATGTTTTTCTTTCAATGATGTAGATTCTTACTGAGTGTGTGCAGCATTTGCAGGAAGTAATGTGGCAGTTAATCTAGGGGATTAAGACTTTTGTACTCCACTAGAAGTTGCTGGGCCTTAATCGTTCAGTATTGTTAAGTGATAGGCTCTAGTTTTTAATCCCATTATCATAGAGCTTGAAACCATCATGGGTTGGCCTAATGGTAAAAAGGGAGACATAGTCTCAATATCTAACTAAAAGGTCTTAGGTTCAATCCATGGTGGCCACGTATCTAGAAATTAATTTCCTACAAGTTTCCTTGACACCCAGATGTTGTAGGGTCAGGTAGGTTGTTCTGTGAGATTAGTCGAGGTGCGCGTAAACTGACTCGGACATTTACGGATATAAAAAAAATTATCACAAAGGCTGAAAATTTTTAGTTACTAAATGCAAGCTTGCAATTAGCATAGATAGTTACTGATTGTCATCTTGCACTTGCTTGCAGATTCTCTAATTTGACAGGAACTGATTACAGTGAAGGGGCAATAGATCTTGCTAGAAGCCTTGCAGAGCGAGATGGTTTTTCCAATATTAACTTTTTGGTATGACTTGGCTTGAGGGTGTTTTGATTACCGTACTTATTCTCTTTAAAAATCTTTGCAAATCTATCTCTCTATCTCTGTTTCTTTTTTTTTTTCCATAACAAGAAACAAAACTTTCTACTGCTGAAATGAAAAACATTCTGTCTCTCTATTGGATATGGTATGGAACATAGTTACTTTCTACAAAATTGATAGAGGTTTATTTTTGGCTTTGTAAGAACATATATATAGTTTTTGGCATATATGTTCCTATTTTTTGTGTTAAAAAAGAATTAAAATGTTTTCTAAACTACATTGTCACATTTCTAATGGGCTATAATATGACTTATCGCTGGTAAATGTCATTGGTTCAAGTTAGATTGAGAACTATTTTAAGAGTAAATTAGATTTTTTTACCATAAATCTTTGTTTACAGAATTAGATCTAAATAGACTAGATTCGCTGTTAGTTTTTCTTTTTTTTGTTCGAGCAAAGGACAGTAATTGACAAATTAGATTCAGGAGACAGATTGGGAATGAAATTGAGGTGGGGGATTACCTGTTTAGTCAATCTCATAAATCCAGCCAATTGTTTGTCAGATTATGTTGCATTGGAGTTTCGAGAAATAATTGTCACATCTTTATATTGTCTATCATTGATACCAAGCATGATTTGAAGTATAGAGATGATTTGTAAAACTTTTAAAAGTTGAGTAAATAAATTAACAAAATACTTATTAAACAAATGGGGATAGTTGTTAAATGGCTGTTAGACCACCAATAATCCACACTTATGTTAGGAGAGGCAGCTCGGGTAATTCACCTAACCAGGGTTAAGTAGTGGTTGGGTGACCTGGGTAATATAAATACTTTTGTAATGTTTTGGGACGACTTATGTTTTTGGATGTGAATTGTGCTGTTTGTAGAGGTTGACCGTAATTTGTTTCTTATTAAGTAAAGATCCCAACTCTCTGTAGCTGTAGAGAGCTTTTACAATGCCCTTGGGTTCATACTAGCTGATTCGATGCCTTTTGACGGTCAAGCACTGTATGGTGCAGTTTTAGCATCGTAAACCCAAATGTAAAATCATCTGTAGATCCAGAAAGAAAGTGGAATTAGAGAGTTTTACGAGCATGAAATTCACGAACTCATTTTCTATTAACCGTCTTAGAGTTATTCAACTCAACGCTTGTGCTACCCCTATAAATGGAAAGTGTCTTGTTGCAAATGTATTGGTATGGTCATGTCTGGTAATTTCTTTTCTTTTACGATTTTGGTAGTTTGTGTATTTTGTTTGGTTTCCTTGCACTTTGAGCGTTAAGTATCTTTTCGTGTATCAATGAAGAACTTTTTTTTCTAAAAAAAGAAGAAATACGATGGACGTGTCTTGGAAATTTTAAAGCTCAACACTTGAGTGATTCATGAATCTTATTGATGGTTTTTCAAAAGCTTCTTTCCTCGAGTTTGGGGTTTGTGCATAGTTTGCTCTTTGTTGGTTTATGGTTGGATTATGGGTTTCTTTGGTAGTGTTTATTAGTAGCCTTTGTTTTACCGTATTTGAAGTAGCCAGGGGGCATGTCGGTTGTAGCCATTTGTTATTCGTAACTTTTCTTTTTCTCTTCATTTTGGAGTTTTATCTTTTGATTTTTATTCCTTTTCTTGTTTCAAAAGAGAGATAGTCAGGAAATAGTAGGCCATTTATTAGATATTTAGAAACGTTTCCTTACTTAGTTTCTGCATGATATTGTTGGATCATCATAATTGTATTGTTTTACTTTTACATGACTTAGGTTGATGATGTTCTTGAGACAAAGTTGGAAGGCCAATTTCAGCTCGTTGTGGATAAAGGGACTTTAGATGCAATAGGATTGCATCCAGATGGTCCTATTAAAAGGTAACAATAAGCAAATACATGCATTCTATAATATAGTTGTATGTGGACTGTTTCACATTGGACAGGTGCTTACTCGTGCTCCCACTGAACATGGGCGGCATGGTAAATTGATAATTGCAGACTTGCAAGTTTCACTCCGATAGATATTACTGGATAGAAAATCGAAGTAATTGGTGTTTCTTTATTTCTTTTTATTATTTCCAGGATCATGTATTGGGAGTCTGTATCGAAACTGGTGGCTCCAGGTGGGGTTTTGGTAAGGCCTTTTTTTATCCCCTCTCACCTATCTGGTAGGAAATAATAATAGTAATAGTAACAATAAGAAGGAATATTTTTAAATATAGCAAAGTGAACTAAAATATTTACAAAATATAGCAAAATTTCACGACTCTATCACTGATACTGCTAGACACTTTATTAGACAAAGCACTGATAGATACTGATTGGAGTCTTGAAATTTTTGCTAATTTACTGTTTACAATAATTACCCTAATAATAATGATGTGATGATGATCTACTTCATGCTAAACATGCCCATCTGAAATTTTAAATTCTAGAGTTTTCCAACATCCAGATATTGTAGCATCTCGCAAATAGTTTAATGAGATGGCCTGAACTTTGCACTTTGTTTAAAACATACATTTATTCTTTTAAAAGGCAATATTACTCTTGATCTTCTAAAAATATTTCAAGACTACCCTTAGAATAGAAGTCCGTTAAAACGTTTGGATGAAATTTGGGACATGGAACAGTCTAGATGTGATTTGAAACGAAAATTGAGACCAACACTGTCACGCTATTCTAGGTTACCATGATAACCCATTCCAGTCCCAATTTCCATCCAAACTTCCAATGGATTTCTTCTCAAAGGTAATTTTGAAGCATTTATAATAAGGTCGTGTAATATTGCAATACCTTTTGAATTGGATCTCTTCTTCCCCCACCCTCCTCCCGTTGTAAATGTAATACATCCATTTCATTGATTTCATATATCAGATGAATTGAAACAAAGGACGAAGTTCAAGAATATTTCTTGCAATTTAGGCATTTGATTGATATCATTATGAGAAATATAAAGTAATATGAACTATTTTTCGTAAATCAACAGAGTGTTGTTTACGATTACAACTCAGTTGAAGTCAAAACTCAGAACAAATCAAATGAAGAACAAACGAGTAAAAGGAAAATCAAGAACAATTAAAAGGAACCTTACACCTCTGCCCTTCCTATGTTTCAAGGAATGTTGCAGACCCTTCACTCAACCCCACAAAATGAACTCTCCTTCCCTCTTTTCCATTTGCCCTCTTATACAACATATTTCTAACTAACTCTGGCTCCGTTATGGTCCCTTTCTCACTGCCTAAGTTTTTCTTCACACGTGTGCTTCCCCTTTCTTACCCTTTCTACTATATTGTAGAATAACAGGGGCTACCAGCATTCTTATGAGATTCTTGAAATGCTTGTGAGCTCCGCCTTCTTTTTCCCATTTACATTTTATGTTCTTTGGCAAATTCGAGATGTTTTATTCTCTTGGAGCTCTAGTGCATAGACATAGCATCCTTCGTTCAGTGAAACTGCGATGCAATTGGGACTGACCCTTTTTTATTACATCTAATGCTCATATTAAATGAGACGTAGAAAAATATGAAACAAGAGATAAGCAACTGGAACTGACTTGTCACTGGTAATTCCTGTGTAGGTGATCACTTCTTGTAACAGCACAAAAGATGAACTTGTGCAAGAAGTTGAAAACTTCAACCAAAGAAGGGTTAATACCTTTGCGGAACCAGAGTCATCTGATGAAACACAACCAGAAGAATTGTTGCCTACATTTCAGTACCTAAGCCATGTTCGTACGTACCCGACATTCACCTTTGGAGGATCTGTTGGATCCCGTGTTGCCACAGTGGCATTTCTTAGAAACTAA

mRNA sequence

ATGACAAAATTCTCTAATTTGACAGGAACTGATTACAGTGAAGGGGCAATAGATCTTGCTAGAAGCCTTGCAGAGCGAGATGGTTTTTCCAATATTAACTTTTTGGTTGATGATGTTCTTGAGACAAAGTTGGAAGGCCAATTTCAGCTCGTTGTGGATAAAGGGACTTTAGATGCAATAGGATTGCATCCAGATGGTCCTATTAAAAGGATCATGTATTGGGAGTCTGTATCGAAACTGGTGGCTCCAGGTGGGGTTTTGGTGATCACTTCTTGTAACAGCACAAAAGATGAACTTGTGCAAGAAGTTGAAAACTTCAACCAAAGAAGGGTTAATACCTTTGCGGAACCAGAGTCATCTGATGAAACACAACCAGAAGAATTGTTGCCTACATTTCAGTACCTAAGCCATGTTCGTACGTACCCGACATTCACCTTTGGAGGATCTGTTGGATCCCGTGTTGCCACAGTGGCATTTCTTAGAAACTAA

Coding sequence (CDS)

ATGACAAAATTCTCTAATTTGACAGGAACTGATTACAGTGAAGGGGCAATAGATCTTGCTAGAAGCCTTGCAGAGCGAGATGGTTTTTCCAATATTAACTTTTTGGTTGATGATGTTCTTGAGACAAAGTTGGAAGGCCAATTTCAGCTCGTTGTGGATAAAGGGACTTTAGATGCAATAGGATTGCATCCAGATGGTCCTATTAAAAGGATCATGTATTGGGAGTCTGTATCGAAACTGGTGGCTCCAGGTGGGGTTTTGGTGATCACTTCTTGTAACAGCACAAAAGATGAACTTGTGCAAGAAGTTGAAAACTTCAACCAAAGAAGGGTTAATACCTTTGCGGAACCAGAGTCATCTGATGAAACACAACCAGAAGAATTGTTGCCTACATTTCAGTACCTAAGCCATGTTCGTACGTACCCGACATTCACCTTTGGAGGATCTGTTGGATCCCGTGTTGCCACAGTGGCATTTCTTAGAAACTAA

Protein sequence

MTKFSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLHPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN*

Homology

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BLAST of Csa3G187220 vs. Swiss-Prot
Match: MET10_MOUSE (Protein-lysine N-methyltransferase Mettl10 OS=Mus musculus GN=Mettl10 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 3.3e-19
Identity = 67/159 (42.14%), Postives = 85/159 (53.46%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVL--ETKLEGQFQLVVDKGTLDAIG 63
           FSN+TG DYS  AI L+ S+ E++G SNIN  V+D L   TKL G F + VDKGT DAI 
Sbjct: 102 FSNITGIDYSPSAIKLSASILEKEGLSNINLKVEDFLNPSTKLSG-FHVCVDKGTYDAIS 161

Query: 64  LHPDGPI-KRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS 123
           L+PD  I KR  Y  S+S+++   G  +ITSCN TK EL+                   S
Sbjct: 162 LNPDNAIEKRKQYVMSLSRVLEVKGFFLITSCNWTKAELLDAF----------------S 221

Query: 124 DETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAF 160
           +  +  E LPT          P F+FGG  G+ VA + F
Sbjct: 222 EGFELFEELPT----------PKFSFGGRSGNTVAALVF 233

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BLAST of Csa3G187220 vs. Swiss-Prot
Match: MET10_HUMAN (Protein-lysine N-methyltransferase METTL10 OS=Homo sapiens GN=METTL10 PE=1 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 8.2e-18
Identity = 51/102 (50.00%), Postives = 68/102 (66.67%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDV--LETKLEGQFQLVVDKGTLDAIG 63
           FSN+TG DYS  AI L+ S+ E++G SNI   V+D   L T+L G F + +DKGT DAI 
Sbjct: 102 FSNITGIDYSPSAIQLSGSIIEKEGLSNIKLKVEDFLNLSTQLSG-FHICIDKGTFDAIS 161

Query: 64  LHPDGPI-KRIMYWESVSKLVAPGGVLVITSCNSTKDELVQE 103
           L+PD  I KR  Y +S+S+++   G  +ITSCN TK+EL+ E
Sbjct: 162 LNPDNAIEKRKQYVKSLSRVLKVKGFFLITSCNWTKEELLNE 202

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BLAST of Csa3G187220 vs. Swiss-Prot
Match: MET10_DANRE (Protein-lysine N-methyltransferase mettl10 OS=Danio rerio GN=mettl10 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.0e-15
Identity = 56/159 (35.22%), Postives = 79/159 (49.69%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVL--ETKLEGQFQLVVDKGTLDAIG 63
           FSNLTG DYS+ A++L  ++   +G  NIN  V+D L   T+L+G F + +DKGT DAI 
Sbjct: 98  FSNLTGIDYSKAALELTTNILVEEGLKNINIQVEDFLNPSTELKG-FDVCIDKGTFDAIS 157

Query: 64  LHP-DGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS 123
           L+P D    +  Y  S+  ++ P G  +ITSCN TK++L+                    
Sbjct: 158 LNPEDREEAKKHYVTSLRAVMRPNGFFIITSCNWTKEQLL-------------------- 217

Query: 124 DETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAF 160
                E   P F+ +  + T P F FGG  G+ V  + F
Sbjct: 218 -----EIFKPGFELVRELPT-PNFQFGGVTGNSVTALVF 229

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BLAST of Csa3G187220 vs. Swiss-Prot
Match: EFM4_YEAST (Protein-lysine N-methyltransferase EFM4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EFM4 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.6e-13
Identity = 53/161 (32.92%), Postives = 81/161 (50.31%), Query Frame = 1


Query: 7   LTGTDYSEGAIDLARSLAERDGFSN-INFLVDDVLETKLE-GQFQLVVDKGTLDAI---G 66
           L G DYSE ++ LA ++AE  G  N I+F   D+     + G++ +V+DKGTLDAI   G
Sbjct: 120 LVGIDYSEESVKLASNIAEATGVDNFISFQQADIFSGDWKPGKYDIVLDKGTLDAISLSG 179

Query: 67  LHPDGPIKRI-MYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS 126
           +  +G +  + +Y   V +++   G+ +ITSCN T+DELV+ +E  N +   T       
Sbjct: 180 MKINGKLDVVDVYAGVVERILKKDGIFLITSCNFTQDELVKIIETDNLKMWKTI------ 239

Query: 127 DETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLR 162
                               YP F FGG  G+ + +VAF++
Sbjct: 240 -------------------KYPVFQFGGVQGATICSVAFVK 255

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BLAST of Csa3G187220 vs. Swiss-Prot
Match: EFM4_SCHPO (Protein-lysine N-methyltransferase efm4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=see1 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.1e-09
Identity = 50/160 (31.25%), Postives = 70/160 (43.75%), Query Frame = 1


Query: 7   LTGTDYSEGAIDLARSLAERDGFSN-INFLVDDVLETK--LEGQFQLVVDKGTLDAIGLH 66
           L G DYSE AI LA+++A    FS+ + F   D+++        + L++DKGT DAI L 
Sbjct: 96  LVGVDYSEAAIVLAKNIARHRQFSDKVKFQQLDIIKDSKFCSKDWDLILDKGTFDAISLS 155

Query: 67  P---DGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS 126
               DG     +Y + V  +++P G+ +ITSCN T  EL                     
Sbjct: 156 GELLDGRPLNSVYVDRVRGMLSPNGIFLITSCNWTIQEL--------------------- 215

Query: 127 DETQPEELLPTFQYLSHVRT-YPTFTFGGSVGSRVATVAF 160
                EE      ++ H     P F F GS GS  + +AF
Sbjct: 216 -----EERFTKNGFIVHSTVPVPVFEFQGSTGSSTSVIAF 229

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BLAST of Csa3G187220 vs. TrEMBL
Match: A0A0A0LA09_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G187220 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 8.4e-86
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 1


Query: 1   MTKFSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAI 60
           MTKFSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAI
Sbjct: 1   MTKFSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAI 60

Query: 61  GLHPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS 120
           GLHPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS
Sbjct: 61  GLHPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS 120

Query: 121 DETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 163
           DETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN
Sbjct: 121 DETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 162

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BLAST of Csa3G187220 vs. TrEMBL
Match: A0A061EGP5_THECC (Protein-lysine N-methyltransferase TCM_019313 OS=Theobroma cacao GN=TCM_019313 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 7.4e-66
Identity = 126/159 (79.25%), Postives = 141/159 (88.68%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLH 63
           F++LTGTDYSEGAIDLARSLA+RDGF NI FLVDD+LETKLE +F+LV+DKGTLDAIGLH
Sbjct: 191 FTDLTGTDYSEGAIDLARSLADRDGFLNIKFLVDDILETKLERRFKLVMDKGTLDAIGLH 250

Query: 64  PDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDET 123
           PDGPIKR+MYW+SVSKLVAPGGVLVITSCN TKDELVQEVENFNQR +    EP + ++ 
Sbjct: 251 PDGPIKRMMYWDSVSKLVAPGGVLVITSCNHTKDELVQEVENFNQRNIEMPQEPNTMEDQ 310

Query: 124 QPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 163
           +    LP FQYL+HVRTYPTF FGGSVGSRVATVAFLRN
Sbjct: 311 ETHRDLPPFQYLNHVRTYPTFMFGGSVGSRVATVAFLRN 349

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BLAST of Csa3G187220 vs. TrEMBL
Match: A0A0B0NHF0_GOSAR (Protein-lysine N-methyltransferase F383_05250 OS=Gossypium arboreum GN=F383_05250 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.3e-65
Identity = 125/159 (78.62%), Postives = 141/159 (88.68%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLH 63
           F++LTGTDYSEGAIDLARSLA+RDGFSNI FLVDD+LETKL+ QFQLV+DKGTLDAIGLH
Sbjct: 188 FTDLTGTDYSEGAIDLARSLADRDGFSNIKFLVDDILETKLDRQFQLVMDKGTLDAIGLH 247

Query: 64  PDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDET 123
           PDGPIKRIMYW+SVSKL+APGG+LVITSCN TKDELVQEVENFNQR +    EP ++ + 
Sbjct: 248 PDGPIKRIMYWDSVSKLLAPGGILVITSCNHTKDELVQEVENFNQRNIAISQEPSATKDQ 307

Query: 124 QPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 163
           +     P F+YL+HVRTYPTF FGGSVGSRVATVAFLRN
Sbjct: 308 ETHRDHPPFRYLNHVRTYPTFMFGGSVGSRVATVAFLRN 346

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BLAST of Csa3G187220 vs. TrEMBL
Match: A0A0D2TH94_GOSRA (Protein-lysine N-methyltransferase B456_007G185600 OS=Gossypium raimondii GN=B456_007G185600 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 3.7e-65
Identity = 124/159 (77.99%), Postives = 141/159 (88.68%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLH 63
           F++LTGTDYSEGAIDLA+SLA+RDGFSNI FLVDD+LETKL+ QFQLV+DKGTLDAIGLH
Sbjct: 188 FTDLTGTDYSEGAIDLAQSLADRDGFSNIKFLVDDILETKLDRQFQLVMDKGTLDAIGLH 247

Query: 64  PDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDET 123
           PDGPIKRIMYW+SVSKL+APGG+LVITSCN TKDELVQEVENFNQR +    EP ++ + 
Sbjct: 248 PDGPIKRIMYWDSVSKLLAPGGILVITSCNHTKDELVQEVENFNQRNIAISQEPSATKDQ 307

Query: 124 QPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 163
           +     P F+YL+HVRTYPTF FGGSVGSRVATVAFLRN
Sbjct: 308 ETHRDHPPFRYLNHVRTYPTFMFGGSVGSRVATVAFLRN 346

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BLAST of Csa3G187220 vs. TrEMBL
Match: A0A0L9TDE4_PHAAN (Protein-lysine N-methyltransferase LR48_Vigan549s009300 OS=Phaseolus angularis GN=LR48_Vigan549s009300 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.6e-63
Identity = 125/159 (78.62%), Postives = 143/159 (89.94%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLH 63
           FS+LTGTDYSE A++LA+SLA+RDGFSNI FLVDDVLETKLE +FQLV+DKGTLDAIGLH
Sbjct: 185 FSDLTGTDYSERAVNLAQSLADRDGFSNIKFLVDDVLETKLEQEFQLVMDKGTLDAIGLH 244

Query: 64  PDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPES-SDE 123
           PDGP+KR+MYW+SVSKLVAPGG+LVITSCNSTKDEL+QEVE+FNQR+++T  E E+  DE
Sbjct: 245 PDGPVKRMMYWDSVSKLVAPGGILVITSCNSTKDELIQEVESFNQRKISTAQEIEAGKDE 304

Query: 124 TQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLR 162
               E  P FQY+SHVRTYPTF FGGSVGSRVATVAFLR
Sbjct: 305 ESCRE--PPFQYVSHVRTYPTFMFGGSVGSRVATVAFLR 341

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BLAST of Csa3G187220 vs. TAIR10
Match: AT1G66680.1 (AT1G66680.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 229.2 bits (583), Expect = 1.9e-60
Identity = 114/166 (68.67%), Postives = 131/166 (78.92%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLH 63
           FS+LTGTDYS+GA++LA+ L++RDGF NI F+VDD+L+TKLE QF+LV+DKGTLDAIGLH
Sbjct: 192 FSDLTGTDYSDGAVELAQHLSQRDGFPNIRFMVDDILDTKLEQQFKLVMDKGTLDAIGLH 251

Query: 64  PDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEP------ 123
           PDGP+KR+MYW+SVSKLVAPGG+LVITSCN TKDELV+EVENFN R+ N           
Sbjct: 252 PDGPVKRVMYWDSVSKLVAPGGILVITSCNHTKDELVEEVENFNIRKSNLCRGDGNDANN 311

Query: 124 --ESSDETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLR 162
              S  E       P F+YLSHVRTYPTF F GSVGSRVATVAFLR
Sbjct: 312 VLSSGSEAASRIDQPPFEYLSHVRTYPTFMFSGSVGSRVATVAFLR 357

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BLAST of Csa3G187220 vs. NCBI nr
Match: gi|700202314|gb|KGN57447.1| (hypothetical protein Csa_3G187220 [Cucumis sativus])

HSP 1 Score: 324.3 bits (830), Expect = 1.2e-85
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 1


Query: 1   MTKFSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAI 60
           MTKFSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAI
Sbjct: 1   MTKFSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAI 60

Query: 61  GLHPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS 120
           GLHPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS
Sbjct: 61  GLHPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS 120

Query: 121 DETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 163
           DETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN
Sbjct: 121 DETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 162

[Close]

BLAST of Csa3G187220 vs. NCBI nr
Match: gi|778679869|ref|XP_011651204.1| (PREDICTED: methyltransferase-like protein 10 isoform X1 [Cucumis sativus])

HSP 1 Score: 319.3 bits (817), Expect = 3.9e-84
Identity = 159/160 (99.38%), Postives = 160/160 (100.00%), Query Frame = 1


Query: 3   KFSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGL 62
           +FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGL
Sbjct: 186 RFSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGL 245

Query: 63  HPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDE 122
           HPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDE
Sbjct: 246 HPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDE 305

Query: 123 TQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 163
           TQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN
Sbjct: 306 TQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 345

[Close]

BLAST of Csa3G187220 vs. NCBI nr
Match: gi|449445949|ref|XP_004140734.1| (PREDICTED: methyltransferase-like protein 10 isoform X2 [Cucumis sativus])

HSP 1 Score: 318.5 bits (815), Expect = 6.6e-84
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLH 63
           FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLH
Sbjct: 186 FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLH 245

Query: 64  PDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDET 123
           PDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDET
Sbjct: 246 PDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDET 305

Query: 124 QPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 163
           QPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN
Sbjct: 306 QPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 344

[Close]

BLAST of Csa3G187220 vs. NCBI nr
Match: gi|659114543|ref|XP_008457104.1| (PREDICTED: methyltransferase-like protein 10 [Cucumis melo])

HSP 1 Score: 303.5 bits (776), Expect = 2.2e-79
Identity = 154/162 (95.06%), Postives = 156/162 (96.30%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINF---LVDDVLETKLEGQFQLVVDKGTLDAI 63
           FSNLTGTDYSEGAIDLARSLAERDGFSNINF   LVDDVLETKLEGQFQLVVDKGTLDAI
Sbjct: 239 FSNLTGTDYSEGAIDLARSLAERDGFSNINFWYDLVDDVLETKLEGQFQLVVDKGTLDAI 298

Query: 64  GLHPDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESS 123
           GLHPDGPIKRIMYW SVSKLVAPGG+LVITSCNSTKDELVQEVENFNQRRVN+F EPESS
Sbjct: 299 GLHPDGPIKRIMYWVSVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFVEPESS 358

Query: 124 DETQPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 163
           DETQP ELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN
Sbjct: 359 DETQPGELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 400

[Close]

BLAST of Csa3G187220 vs. NCBI nr
Match: gi|590652345|ref|XP_007033125.1| (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein isoform 1 [Theobroma cacao])

HSP 1 Score: 258.1 bits (658), Expect = 1.1e-65
Identity = 126/159 (79.25%), Postives = 141/159 (88.68%), Query Frame = 1


Query: 4   FSNLTGTDYSEGAIDLARSLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLH 63
           F++LTGTDYSEGAIDLARSLA+RDGF NI FLVDD+LETKLE +F+LV+DKGTLDAIGLH
Sbjct: 191 FTDLTGTDYSEGAIDLARSLADRDGFLNIKFLVDDILETKLERRFKLVMDKGTLDAIGLH 250

Query: 64  PDGPIKRIMYWESVSKLVAPGGVLVITSCNSTKDELVQEVENFNQRRVNTFAEPESSDET 123
           PDGPIKR+MYW+SVSKLVAPGGVLVITSCN TKDELVQEVENFNQR +    EP + ++ 
Sbjct: 251 PDGPIKRMMYWDSVSKLVAPGGVLVITSCNHTKDELVQEVENFNQRNIEMPQEPNTMEDQ 310

Query: 124 QPEELLPTFQYLSHVRTYPTFTFGGSVGSRVATVAFLRN 163
           +    LP FQYL+HVRTYPTF FGGSVGSRVATVAFLRN
Sbjct: 311 ETHRDLPPFQYLNHVRTYPTFMFGGSVGSRVATVAFLRN 349

The following BLAST results are available for this feature:

BLAST of Csa3G187220 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MET10_MOUSE	3.3e-19	42.14	Protein-lysine N-methyltransferase Mettl10 OS=Mus musculus GN=Mettl10 PE=1 SV=1	[more]
MET10_HUMAN	8.2e-18	50.00	Protein-lysine N-methyltransferase METTL10 OS=Homo sapiens GN=METTL10 PE=1 SV=2	[more]
MET10_DANRE	1.0e-15	35.22	Protein-lysine N-methyltransferase mettl10 OS=Danio rerio GN=mettl10 PE=2 SV=1	[more]
EFM4_YEAST	1.6e-13	32.92	Protein-lysine N-methyltransferase EFM4 OS=Saccharomyces cerevisiae (strain ATCC...	[more]
EFM4_SCHPO	3.1e-09	31.25	Protein-lysine N-methyltransferase efm4 OS=Schizosaccharomyces pombe (strain 972...	[more]

BLAST of Csa3G187220 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA09_CUCSA	8.4e-86	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G187220 PE=4 SV=1	[more]
A0A061EGP5_THECC	7.4e-66	79.25	Protein-lysine N-methyltransferase TCM_019313 OS=Theobroma cacao GN=TCM_019313 P...	[more]
A0A0B0NHF0_GOSAR	1.3e-65	78.62	Protein-lysine N-methyltransferase F383_05250 OS=Gossypium arboreum GN=F383_0525...	[more]
A0A0D2TH94_GOSRA	3.7e-65	77.99	Protein-lysine N-methyltransferase B456_007G185600 OS=Gossypium raimondii GN=B45...	[more]
A0A0L9TDE4_PHAAN	2.6e-63	78.62	Protein-lysine N-methyltransferase LR48_Vigan549s009300 OS=Phaseolus angularis G...	[more]

BLAST of Csa3G187220 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G66680.1	1.9e-60	68.67	S-adenosyl-L-methionine-dependent methyltransferases superfamily pro...	[more]

BLAST of Csa3G187220 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202314\|gb\|KGN57447.1\|	1.2e-85	100.00	hypothetical protein Csa_3G187220 [Cucumis sativus]	[more]
gi\|778679869\|ref\|XP_011651204.1\|	3.9e-84	99.38	PREDICTED: methyltransferase-like protein 10 isoform X1 [Cucumis sativus]	[more]
gi\|449445949\|ref\|XP_004140734.1\|	6.6e-84	100.00	PREDICTED: methyltransferase-like protein 10 isoform X2 [Cucumis sativus]	[more]
gi\|659114543\|ref\|XP_008457104.1\|	2.2e-79	95.06	PREDICTED: methyltransferase-like protein 10 [Cucumis melo]	[more]
gi\|590652345\|ref\|XP_007033125.1\|	1.1e-65	79.25	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein isoform...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR025714	Methyltranfer_dom
IPR026635	Efm4/METTL10

Vocabulary: **Molecular Function**
Term	Definition
GO:0008168	methyltransferase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0018022	peptidyl-lysine methylation
biological_process	GO:0008150	biological_process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0016279	protein-lysine N-methyltransferase activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU155654	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa3G187220.1	Csa3G187220.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU155654	CU155654	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025714	Methyltransferase domain	PFAM	PF13847	Methyltransf_31	coord: 6..116 score: 1.0
IPR026635	Protein-lysine N-methyltransferase Efm4	PANTHER	PTHR12843	UNCHARACTERIZED	coord: 4..106 score: 2.5E-72coord: 124..161 score: 2.5
None	No IPR available	PANTHER	PTHR12843:SF5	METHYLTRANSFERASE-LIKE PROTEIN 10	coord: 4..106 score: 2.5E-72coord: 124..161 score: 2.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa3G187220	MELO3C020315	Melon (DHL92) v3.5.1	cumeB239
Csa3G187220	ClCG05G010730	Watermelon (Charleston Gray)	cuwcgB230
Csa3G187220	Lsi05G002780	Bottle gourd (USVL1VR-Ls)	culsiB211
Csa3G187220	MELO3C020314.2	Melon (DHL92) v3.6.1	cumedB237
Csa3G187220	Cla97C05G093050	Watermelon (97103) v2	cuwmbB215
Csa3G187220	Bhi01G001324	Wax gourd	cuwgoB283
Csa3G187220	CSPI03G17610	Wild cucumber (PI 183967)	cpicuB120
Csa3G187220	Cucsa.108470	Cucumber (Gy14) v1	cgycuB132
Csa3G187220	CmaCh01G003410	Cucurbita maxima (Rimu)	cmacuB458
Csa3G187220	CmaCh14G008640	Cucurbita maxima (Rimu)	cmacuB245
Csa3G187220	CmoCh14G008780	Cucurbita moschata (Rifu)	cmocuB232
Csa3G187220	CmoCh01G003580	Cucurbita moschata (Rifu)	cmocuB445
Csa3G187220	Cp4.1LG03g05730	Cucurbita pepo (Zucchini)	cpecuB603
Csa3G187220	Cp4.1LG02g16630	Cucurbita pepo (Zucchini)	cpecuB550
Csa3G187220	CsGy3G017660	Cucumber (Gy14) v2	cgybcuB109
Csa3G187220	Carg24236	Silver-seed gourd	carcuB0196
Csa3G187220	Carg07433	Silver-seed gourd	carcuB0781

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa3G187220	Melon (DHL92) v3.5.1	cumeB251
Csa3G187220	Watermelon (97103) v1	cuwmB243
Csa3G187220	Melon (DHL92) v3.6.1	cumedB238