Csa3G171850 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa3G171850
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Rapid alkalinization factor preproprotein; contains IPR008801 (Rapid ALkalinization Factor)
Location	Chr3 : 11505686 .. 11506019 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTACAAACTTGCTTTCTTCTTCTTCTCTTTCTCAGCTTCCTTCTTCAAGATCTTTGTCTTGTTCGAGGTTCCTCGATGCATTCTTGCAATGGCTCCATAGCCGAGTGTGCTAACGAGGAGGAGATGTTGATGGAGTCGGAGATAACTCGAAGGTTTCTCGCACAACAAAAGAAGAACTACATCTCTTATCCAACTTTGAAGAAGGATCGCCCAGCTTGCGATGGCGCTAGTGGCCAACCTTACACTAAGAGCGGAAGTTGCGTTCCGTCACAGGCTAATCCTTACAATCCAGGTTGCTCTAACATATATTATTGTAGGCATTGATTAA

mRNA sequence

ATGTCTTTACAAACTTGCTTTCTTCTTCTTCTCTTTCTCAGCTTCCTTCTTCAAGATCTTTGTCTTGTTCGAGGTTCCTCGATGCATTCTTGCAATGGCTCCATAGCCGAGTGTGCTAACGAGGAGGAGATGTTGATGGAGTCGGAGATAACTCGAAGGTTTCTCGCACAACAAAAGAAGAACTACATCTCTTATCCAACTTTGAAGAAGGATCGCCCAGCTTGCGATGGCGCTAGTGGCCAACCTTACACTAAGAGCGGAAGTTGCGTTCCGTCACAGGCTAATCCTTACAATCCAGGCATTGATTAA

Coding sequence (CDS)

ATGTCTTTACAAACTTGCTTTCTTCTTCTTCTCTTTCTCAGCTTCCTTCTTCAAGATCTTTGTCTTGTTCGAGGTTCCTCGATGCATTCTTGCAATGGCTCCATAGCCGAGTGTGCTAACGAGGAGGAGATGTTGATGGAGTCGGAGATAACTCGAAGGTTTCTCGCACAACAAAAGAAGAACTACATCTCTTATCCAACTTTGAAGAAGGATCGCCCAGCTTGCGATGGCGCTAGTGGCCAACCTTACACTAAGAGCGGAAGTTGCGTTCCGTCACAGGCTAATCCTTACAATCCAGGCATTGATTAA

Protein sequence

MSLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACDGASGQPYTKSGSCVPSQANPYNPGID*

Homology

[Close]

BLAST of Csa3G171850 vs. Swiss-Prot
Match: RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.4e-07
Identity = 33/76 (43.42%), Postives = 39/76 (51.32%), Query Frame = 1


Query: 25  GSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACDGASGQPYT 84
           G +   C+GSIAEC   EE  M+SEI RR LA  K  YISY +LK++   C       Y 
Sbjct: 41  GDNGSGCHGSIAECIGAEEEEMDSEINRRILATTK--YISYQSLKRNSVPCSRRGASYYN 100

Query: 85  KSGSCVPSQANPYNPG 101
                  +QANPY+ G
Sbjct: 101 CQNG---AQANPYSRG 111

[Close]

BLAST of Csa3G171850 vs. Swiss-Prot
Match: RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 7.0e-07
Identity = 36/84 (42.86%), Postives = 45/84 (53.57%), Query Frame = 1


Query: 19  DLCLVRGSSMHS-CNGSIAEC-ANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACD 78
           D      SS+ S C GSIAEC A EEEM  +S+I+RR LAQ+K  YISY  ++++   C 
Sbjct: 32  DFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRILAQKK--YISYGAMRRNSVPCS 91

Query: 79  GASGQPYTKSGSCVPSQANPYNPG 101
                 Y        +QANPY+ G
Sbjct: 92  RRGASYYNCQRG---AQANPYSRG 110

[Close]

BLAST of Csa3G171850 vs. TrEMBL
Match: A0A0A0L624_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171850 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 2.9e-52
Identity = 102/102 (100.00%), Postives = 102/102 (100.00%), Query Frame = 1


Query: 1   MSLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKK 60
           MSLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKK
Sbjct: 1   MSLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKK 60

Query: 61  NYISYPTLKKDRPACDGASGQPYTKSGSCVPSQANPYNPGID 103
           NYISYPTLKKDRPACDGASGQPYTKSGSCVPSQANPYNPGID
Sbjct: 61  NYISYPTLKKDRPACDGASGQPYTKSGSCVPSQANPYNPGID 102

[Close]

BLAST of Csa3G171850 vs. TrEMBL
Match: A0A0A0L9A8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171840 PE=4 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 3.8e-36
Identity = 84/102 (82.35%), Postives = 85/102 (83.33%), Query Frame = 1


Query: 1   MSLQTCFLLLLFLS-FLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQK 60
           MSLQTCFLLLLFL  FL QDLCLVR SS HSCNGSIA CANEEEMLMESEITRRFL QQK
Sbjct: 17  MSLQTCFLLLLFLFLFLFQDLCLVRASSTHSCNGSIAACANEEEMLMESEITRRFLEQQK 76

Query: 61  KNYISYPTLKKDRPACDGAS-GQPYTKSGSCVPSQANPYNPG 101
           K YIS   LKKD PACDGAS GQPYT+SGSC P  ANPYN G
Sbjct: 77  K-YISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRG 117

[Close]

BLAST of Csa3G171850 vs. TrEMBL
Match: A0A0S3R1D3_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G214200 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.6e-18
Identity = 49/101 (48.51%), Postives = 69/101 (68.32%), Query Frame = 1


Query: 3   LQTCFLLLLFLSFLLQDLCLVRGSSMH--SCNGSIAECANEEEMLMESEITRRFLAQQKK 62
           ++ CF+ L+F SF+    C V   + H  +CNGSIAEC  E+E+LMESE +RRFL Q+K 
Sbjct: 9   VRLCFIYLIFFSFMRLSSCTVLSLTSHVSTCNGSIAECNQEDELLMESETSRRFLEQKK- 68

Query: 63  NYISYPTLKKDRPACD-GASGQPYTKSGSCVPSQANPYNPG 101
            YIS   L++D+P C+ G SG+ Y+ SG C+P  +NP+N G
Sbjct: 69  -YISNGALQRDKPVCNGGGSGEAYSNSGGCLPPPSNPHNRG 107

[Close]

BLAST of Csa3G171850 vs. TrEMBL
Match: C6T1A5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G151400 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 3.1e-17
Identity = 48/98 (48.98%), Postives = 66/98 (67.35%), Query Frame = 1


Query: 6   CFLLLLFLSFLLQDLCLVRGSSMHS--CNGSIAECANEEEMLMESEITRRFLAQQKKNYI 65
           CF   +  SF+    C V     H+  CNGSIAEC  E+E+LMESEI+RRFL +QK++YI
Sbjct: 12  CFCYFMLFSFMHFSSCTVFSLKSHASTCNGSIAECNQEDELLMESEISRRFL-EQKRSYI 71

Query: 66  SYPTLKKDRPACD-GASGQPYTKSGSCVPSQANPYNPG 101
           S   L++D+P C+ G SG+ Y+K+G C+P  +NP N G
Sbjct: 72  SNGALQRDKPVCNGGGSGEAYSKTGGCLPPPSNPQNRG 108

[Close]

BLAST of Csa3G171850 vs. TrEMBL
Match: V7C8L1_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G051400g PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.5e-16
Identity = 46/102 (45.10%), Postives = 67/102 (65.69%), Query Frame = 1


Query: 2   SLQTCFLLLLFLSFLLQDLCLVRGSSMH--SCNGSIAECANEEEMLMESEITRRFLAQQK 61
           +++ CF   +  SF+    C V   + H   CNG+IA+C  E+E+LMESEI+RRFL Q  
Sbjct: 7   TIRLCFFYFMLFSFMHFSCCTVLSFTSHVGRCNGTIADCNQEDELLMESEISRRFLEQ-- 66

Query: 62  KNYISYPTLKKDRPACD-GASGQPYTKSGSCVPSQANPYNPG 101
           K+YIS   L++D+P C+ G SG+ Y+ SG C+P  +NP+N G
Sbjct: 67  KSYISNGALQRDKPVCNGGGSGEAYSNSGGCLPPPSNPHNRG 106

[Close]

BLAST of Csa3G171850 vs. TAIR10
Match: AT1G02900.1 (AT1G02900.1 rapid alkalinization factor 1)

HSP 1 Score: 56.2 bits (134), Expect = 1.4e-08
Identity = 33/76 (43.42%), Postives = 39/76 (51.32%), Query Frame = 1


Query: 25  GSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACDGASGQPYT 84
           G +   C+GSIAEC   EE  M+SEI RR LA  K  YISY +LK++   C       Y 
Sbjct: 41  GDNGSGCHGSIAECIGAEEEEMDSEINRRILATTK--YISYQSLKRNSVPCSRRGASYYN 100

Query: 85  KSGSCVPSQANPYNPG 101
                  +QANPY+ G
Sbjct: 101 CQNG---AQANPYSRG 111

[Close]

BLAST of Csa3G171850 vs. TAIR10
Match: AT3G05490.1 (AT3G05490.1 ralf-like 22)

HSP 1 Score: 54.7 bits (130), Expect = 4.0e-08
Identity = 36/84 (42.86%), Postives = 45/84 (53.57%), Query Frame = 1


Query: 19  DLCLVRGSSMHS-CNGSIAEC-ANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACD 78
           D      SS+ S C GSIAEC A EEEM  +S+I+RR LAQ+K  YISY  ++++   C 
Sbjct: 32  DFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRILAQKK--YISYGAMRRNSVPCS 91

Query: 79  GASGQPYTKSGSCVPSQANPYNPG 101
                 Y        +QANPY+ G
Sbjct: 92  RRGASYYNCQRG---AQANPYSRG 110

[Close]

BLAST of Csa3G171850 vs. NCBI nr
Match: gi|700202095|gb|KGN57228.1| (hypothetical protein Csa_3G171850 [Cucumis sativus])

HSP 1 Score: 212.2 bits (539), Expect = 4.2e-52
Identity = 102/102 (100.00%), Postives = 102/102 (100.00%), Query Frame = 1


Query: 1   MSLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKK 60
           MSLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKK
Sbjct: 1   MSLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKK 60

Query: 61  NYISYPTLKKDRPACDGASGQPYTKSGSCVPSQANPYNPGID 103
           NYISYPTLKKDRPACDGASGQPYTKSGSCVPSQANPYNPGID
Sbjct: 61  NYISYPTLKKDRPACDGASGQPYTKSGSCVPSQANPYNPGID 102

[Close]

BLAST of Csa3G171850 vs. NCBI nr
Match: gi|778679218|ref|XP_004147646.2| (PREDICTED: protein RALF-like 32 [Cucumis sativus])

HSP 1 Score: 158.7 bits (400), Expect = 5.5e-36
Identity = 84/102 (82.35%), Postives = 85/102 (83.33%), Query Frame = 1


Query: 1   MSLQTCFLLLLFLS-FLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQK 60
           MSLQTCFLLLLFL  FL QDLCLVR SS HSCNGSIA CANEEEMLMESEITRRFL QQK
Sbjct: 17  MSLQTCFLLLLFLFLFLFQDLCLVRASSTHSCNGSIAACANEEEMLMESEITRRFLEQQK 76

Query: 61  KNYISYPTLKKDRPACDGAS-GQPYTKSGSCVPSQANPYNPG 101
           K YIS   LKKD PACDGAS GQPYT+SGSC P  ANPYN G
Sbjct: 77  K-YISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRG 117

[Close]

BLAST of Csa3G171850 vs. NCBI nr
Match: gi|659077106|ref|XP_008439036.1| (PREDICTED: protein RALF-like 32 [Cucumis melo])

HSP 1 Score: 152.9 bits (385), Expect = 3.0e-34
Identity = 78/101 (77.23%), Postives = 84/101 (83.17%), Query Frame = 1


Query: 1   MSLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKK 60
           MSLQ CFLLLL   FLLQDLCLVR SS HSCNGSIAEC +E+E+LMESEI+RRFLAQQKK
Sbjct: 10  MSLQPCFLLLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEILMESEISRRFLAQQKK 69

Query: 61  NYISYPTLKKDRPACDGAS-GQPYTKSGSCVPSQANPYNPG 101
            YIS   LKKD PACDGAS G+PYTKSGSC P  ANPY+ G
Sbjct: 70  -YISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRG 109

[Close]

BLAST of Csa3G171850 vs. NCBI nr
Match: gi|951073329|ref|XP_014492116.1| (PREDICTED: protein RALF-like 32 [Vigna radiata var. radiata])

HSP 1 Score: 99.8 bits (247), Expect = 3.0e-18
Identity = 50/101 (49.50%), Postives = 68/101 (67.33%), Query Frame = 1


Query: 3   LQTCFLLLLFLSFLLQDLCLVRGSSMH--SCNGSIAECANEEEMLMESEITRRFLAQQKK 62
           ++ CF  L+F  F+    C V   + H  +CNGSIAEC  E+E+LMESEI+RRFL Q+K 
Sbjct: 9   IRLCFFNLMFFGFMRLSSCTVLSLTPHVSTCNGSIAECNQEDELLMESEISRRFLEQKK- 68

Query: 63  NYISYPTLKKDRPAC-DGASGQPYTKSGSCVPSQANPYNPG 101
            YIS   L++D+P C DG SG+ Y+ SG C+P  +NP+N G
Sbjct: 69  -YISNGALQRDKPVCNDGGSGEAYSNSGGCLPPPSNPHNRG 107

[Close]

BLAST of Csa3G171850 vs. NCBI nr
Match: gi|965658049|dbj|BAT74468.1| (hypothetical protein VIGAN_01214200 [Vigna angularis var. angularis])

HSP 1 Score: 99.0 bits (245), Expect = 5.2e-18
Identity = 49/101 (48.51%), Postives = 69/101 (68.32%), Query Frame = 1


Query: 3   LQTCFLLLLFLSFLLQDLCLVRGSSMH--SCNGSIAECANEEEMLMESEITRRFLAQQKK 62
           ++ CF+ L+F SF+    C V   + H  +CNGSIAEC  E+E+LMESE +RRFL Q+K 
Sbjct: 9   VRLCFIYLIFFSFMRLSSCTVLSLTSHVSTCNGSIAECNQEDELLMESETSRRFLEQKK- 68

Query: 63  NYISYPTLKKDRPACD-GASGQPYTKSGSCVPSQANPYNPG 101
            YIS   L++D+P C+ G SG+ Y+ SG C+P  +NP+N G
Sbjct: 69  -YISNGALQRDKPVCNGGGSGEAYSNSGGCLPPPSNPHNRG 107

The following BLAST results are available for this feature:

BLAST of Csa3G171850 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF1_ARATH	2.4e-07	43.42	Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1	[more]
RLF22_ARATH	7.0e-07	42.86	Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1	[more]

BLAST of Csa3G171850 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L624_CUCSA	2.9e-52	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171850 PE=4 SV=1	[more]
A0A0A0L9A8_CUCSA	3.8e-36	82.35	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171840 PE=4 SV=1	[more]
A0A0S3R1D3_PHAAN	3.6e-18	48.51	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G214200 PE=...	[more]
C6T1A5_SOYBN	3.1e-17	48.98	Uncharacterized protein OS=Glycine max GN=GLYMA_05G151400 PE=2 SV=1	[more]
V7C8L1_PHAVU	1.5e-16	45.10	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G051400g PE=4 SV=1	[more]

BLAST of Csa3G171850 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G02900.1	1.4e-08	43.42	rapid alkalinization factor 1	[more]
AT3G05490.1	4.0e-08	42.86	ralf-like 22	[more]

BLAST of Csa3G171850 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202095\|gb\|KGN57228.1\|	4.2e-52	100.00	hypothetical protein Csa_3G171850 [Cucumis sativus]	[more]
gi\|778679218\|ref\|XP_004147646.2\|	5.5e-36	82.35	PREDICTED: protein RALF-like 32 [Cucumis sativus]	[more]
gi\|659077106\|ref\|XP_008439036.1\|	3.0e-34	77.23	PREDICTED: protein RALF-like 32 [Cucumis melo]	[more]
gi\|951073329\|ref\|XP_014492116.1\|	3.0e-18	49.50	PREDICTED: protein RALF-like 32 [Vigna radiata var. radiata]	[more]
gi\|965658049\|dbj\|BAT74468.1\|	5.2e-18	48.51	hypothetical protein VIGAN_01214200 [Vigna angularis var. angularis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007154	cell communication
biological_process	GO:0044700	single organism signaling
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa3G171850.1	Csa3G171850.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 42..100 score: 2.1
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 3..98 score: 8.6
None	No IPR available	PANTHER	PTHR33136:SF3	PROTEIN RALF-LIKE 32	coord: 3..98 score: 8.6

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa3G171850	Silver-seed gourd	carcuB0714
Csa3G171850	Silver-seed gourd	carcuB1054
Csa3G171850	Watermelon (97103) v2	cuwmbB204
Csa3G171850	Wax gourd	cuwgoB269
Csa3G171850	Cucumber (Gy14) v1	cgycuB366
Csa3G171850	Cucurbita maxima (Rimu)	cmacuB253
Csa3G171850	Cucurbita maxima (Rimu)	cmacuB814
Csa3G171850	Cucurbita moschata (Rifu)	cmocuB239
Csa3G171850	Cucurbita moschata (Rifu)	cmocuB806
Csa3G171850	Melon (DHL92) v3.5.1	cumeB236
Csa3G171850	Watermelon (Charleston Gray)	cuwcgB222
Csa3G171850	Watermelon (97103) v1	cuwmB233
Csa3G171850	Cucurbita pepo (Zucchini)	cpecuB609
Csa3G171850	Cucurbita pepo (Zucchini)	cpecuB859
Csa3G171850	Bottle gourd (USVL1VR-Ls)	culsiB210
Csa3G171850	Cucumber (Gy14) v2	cgybcuB109
Csa3G171850	Melon (DHL92) v3.6.1	cumedB223