Csa3G110020 (gene) Cucumber (Chinese Long) v2

BLAST of Csa3G110020 vs. Swiss-Prot
Match: MACP2_ARATH (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 1.8e-248
Identity = 431/601 (71.71%), Postives = 496/601 (82.53%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGH-EIVLPGGI 60
           MAL++  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I EG  + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  LISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
            I NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERL------- 240
           +KMGGKDVIY KQQHSS LQP +   +QK LKE+ADKRF E    +  GSER+       
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPED---LQKRLKEVADKRFVEASVVHNTGSERVQASSKVE 240

Query: 241 ----------------YNNDKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIP 300
                           Y N +D V +CKRRGG+D  +L H+EWLQTVQ+EPDVISMSFIP
Sbjct: 241 TKEQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 300

Query: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHN 360
           ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ +
Sbjct: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 360

Query: 361 LASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTE 420
            ASLQFS  G KLYVNTTPVDVG+RP+TG+RLYLEG+RSN L+IHLQHLSSLPKI+QL +
Sbjct: 361 CASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLED 420

Query: 421 DPNGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLR 480
           D N + RQ+S+DRRY EKV  K +SHVCT PVE+D+DLS+VTGAQL V + G +NVLFLR
Sbjct: 421 DLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLR 480

Query: 481 LRFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPG 540
           L FS V+GAT+V+ +EW+ + G A KSGLISTLISHHF+   KPPP+PADVNINSA+YPG
Sbjct: 481 LCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPG 540

Query: 541 GPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPD 578
           GPPVP QAPKLLKFVDT+EM RGPQE+PGYWVVSGARL+VEKG+ISL+VK+SL T IL D
Sbjct: 541 GPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGD 598

BLAST of Csa3G110020 vs. Swiss-Prot
Match: NSL1_ARATH (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana GN=NSL1 PE=2 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 1.8e-168
Identity = 318/603 (52.74%), Postives = 408/603 (67.66%), Query Frame = 1

Query: 10  AAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSIK 69
           AAE A+  IG GYD+  D+R   CK    GSRL+EID     +++ PGGI+++NV  SIK
Sbjct: 14  AAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK 73

Query: 70  CDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAF 129
           CDKGERTR RSD+LSF QMSE+FNQ+M L+GKIPSG FN MF FS CW KDA++ K LA+
Sbjct: 74  CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAY 133

Query: 130 DGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIY 189
           DG FI+LY+V + + Q+ L D V++ VPSSWD AALA FIE +GTH++VG+ MGGKDVI+
Sbjct: 134 DGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIH 193

Query: 190 MKQQHSSTLQPSEPSEVQKSLKEMADKRF-------------------SETIAQYGM--- 249
           +KQ   S     EP E+QK LK   D+RF                    E + Q+G+   
Sbjct: 194 VKQMRKSN---HEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPF 253

Query: 250 -----GSERLYNNDKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLN 309
                 +  ++  +++I+ +C RRGG D +   H  WL TV   P+VISM F+PITSLL+
Sbjct: 254 GTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITSLLS 313

Query: 310 GVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQF 369
           G+PG+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R + +  SLQF
Sbjct: 314 GLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQF 373

Query: 370 SLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNF 429
           SLMG KLYVNT+ VD G RPVTGLR +LEGK+ N L+IHLQHLS+ P    L+ D     
Sbjct: 374 SLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEP 433

Query: 430 RQDSYDRRYLEKVQSKYFSHVCTAPVE-----ADEDLSIVTGAQLQVVNQGMRNVLFLRL 489
            ++  ++ Y   V+   FSHVCT PV+     +D+  SIVT A L+V   GMR VLFLRL
Sbjct: 434 IEEPVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLFLRL 493

Query: 490 RFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPP---KP-ADVNINSAV 549
            F S+  + V R++ W+     + KSG+ S +IS   ST + P P   KP + ++INSAV
Sbjct: 494 GF-SLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDINSAV 553

Query: 550 YPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVI 577
           YP GP  PV+ PKLL  VDT E+ RGP+E PGYWVV+GA+L VE G+IS++ K+SLLTVI
Sbjct: 554 YPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLLTVI 609

BLAST of Csa3G110020 vs. Swiss-Prot
Match: CAD1_ARATH (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana GN=CAD1 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 4.1e-128
Identity = 273/577 (47.31%), Postives = 353/577 (61.18%), Query Frame = 1

Query: 14  AIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSIKCDKG 73
           AI ++GRG+D+T D+RL YCKG   GSRL+ I+EG   ++ L  G L+ NVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAFDGVF 133
                R  V SF +M+E+FN    + G IP G FN MF ++G WQ DAA+TK+LA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 ITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIYMKQQ 193
           I LY V L K  +VL + +R+AVPSSWDPA+LA FIE +GTHI+  + +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKDIVPICKRRGGSDRIS 253
            SS   P   SE++  + +M   RF E  +Q   G   L   DKDI  I +RRGG D + 
Sbjct: 204 QSS---PLPVSEIENYVNDMIKHRFHEAESQSITGP--LKYKDKDITVIFRRRGGDD-LE 263

Query: 254 LPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEF 313
             H  W +TV   PD+I+M+F PI SLL GVPG   L+ AI LYL YKPPIE+L  FL++
Sbjct: 264 QSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFLDY 323

Query: 314 QLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGK 373
           Q+ R WAP  S L    QRK+   +SLQFSLMG KL+++   V VGR+PVTGLRL LEG 
Sbjct: 324 QIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGS 383

Query: 374 RSNCLSIHLQHLSSLPKIFQLTED---PNGNFR---QDSYDRRYLEKVQSKYFSHVCTAP 433
           + N LSIHLQHL SLPKI Q   D   P G  +    +  D R+ E ++ K FSHV T+P
Sbjct: 384 KQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSP 443

Query: 434 VEADE----DLS---IVTGAQLQVVNQGMRNVLFLRLRFSSVLGATVVRQAEWEGSPGLA 493
           +E  E    DLS   IVTGAQL V + G +NVL L+L FS V G T +R++ W+ +P   
Sbjct: 444 IEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCT-IRRSVWDHTP--V 503

Query: 494 AKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGP-------PVPVQAPKLLKFVDT 553
           A SG +                            PGGP        V  Q+ KL K VD+
Sbjct: 504 ASSGRLE---------------------------PGGPSTSSSTEEVSGQSGKLAKIVDS 559

Query: 554 TEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLL 571
           +EM +GPQ+ PG+W+V+GA+L VEKG+I LRVK+SLL
Sbjct: 564 SEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Csa3G110020 vs. Swiss-Prot
Match: MACP1_ARATH (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.3e-73
Identity = 162/364 (44.51%), Postives = 219/364 (60.16%), Query Frame = 1

Query: 234 NNDKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHA 293
           N+   I  IC +RGG  R    H EWL TV  +PD I+ +FIPITSLL  VPGSG LSHA
Sbjct: 263 NSQNGITVICAKRGGDGRAK-SHSEWLITVPDKPDAINFNFIPITSLLKDVPGSGLLSHA 322

Query: 294 INLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS----LQFSLMGSKL 353
           ++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      N+AS    L  + MG KL
Sbjct: 323 MSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFG---AAPNMASAYPALHINFMGPKL 382

Query: 354 YVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIF--QLTEDPNGNFRQDSY 413
           YVNTTPV   + PVTG+R +LEGK+ N L+IHLQHL +       ++T++          
Sbjct: 383 YVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHIWRGSDQIT 442

Query: 414 DR-RYLEKVQSKYFSHVCTAPVEADED--------------LSIVTGAQLQVVNQGMRNV 473
           D  RY E +  K FSHVCT PV+ D +                IVTGAQL+V   G ++V
Sbjct: 443 DNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLEVKKHGSKSV 502

Query: 474 LFLRLRFSSVLGATVVRQAEWEGSPGLAAKSGLISTLI------SHHFSTVMKPPPKPAD 533
           L LRLR++ V    VV+ +   G  G + KSG+ S++       S H + + K      +
Sbjct: 503 LHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHNMIQK---DKNE 562

Query: 534 VNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVK 571
           V ++S V+PGGPPVP    K++KFVD +++ RGPQ +PG+W+V+G RL ++KG++ L VK
Sbjct: 563 VVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDKGKLCLHVK 618

HSP 2 Score: 197.6 bits (501), Expect = 3.8e-49
Identity = 101/213 (47.42%), Postives = 139/213 (65.26%), Query Frame = 1

Query: 12  EIAIGSIGRGYDITIDLRLKYCK---GNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSI 71
           E A+ S+G+G+D+T D RLKYCK   G+    RL+ +D+    E+ +PG  +  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEF-SGCWQKDAANTKNL 131
            CDKGERTRFRSD+L F +MSE FNQ  ++TGKIPSG+FN  F F SG W  DAAN K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 AFDGVFITLYTVALEK-SQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKD 191
             D   +TL+ + +   +++ L D VR AVPSSWDP  LARFIE +GTH+I G+ +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFS 220
           V+ ++Q  SS L   +   ++  L ++ D+ F+
Sbjct: 190 VVVVRQDKSSDL---DNDLLRHHLYDLGDQLFT 219

BLAST of Csa3G110020 vs. TrEMBL
Match: A0A0A0L5E1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G110020 PE=4 SV=1)

HSP 1 Score: 1172.5 bits (3032), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKDIV 240
           KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKDIV
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKDIV 240

Query: 241 PICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRY 300
           PICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRY
Sbjct: 241 PICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRY 300

Query: 301 KPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGR 360
           KPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGR
Sbjct: 301 KPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGR 360

Query: 361 RPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFRQDSYDRRYLEKVQSKYF 420
           RPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFRQDSYDRRYLEKVQSKYF
Sbjct: 361 RPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFRQDSYDRRYLEKVQSKYF 420

Query: 421 SHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRFSSVLGATVVRQAEWEGSPGLA 480
           SHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRFSSVLGATVVRQAEWEGSPGLA
Sbjct: 421 SHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRFSSVLGATVVRQAEWEGSPGLA 480

Query: 481 AKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGP 540
           AKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGP
Sbjct: 481 AKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGP 540

Query: 541 QETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEINDN 584
           QETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEINDN
Sbjct: 541 QETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEINDN 583

BLAST of Csa3G110020 vs. TrEMBL
Match: M5XBD0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003112mg PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 7.5e-278
Identity = 485/601 (80.70%), Postives = 528/601 (87.85%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKV   KAAEIAI SIG GYDI+IDLRLKYCKG  + SRLIEID+  G EIVLPGGI 
Sbjct: 1   MALKVPAPKAAEIAIASIGHGYDISIDLRLKYCKGESYTSRLIEIDDDGGREIVLPGGIT 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQE++LTGKIPSG FN+MFEFSGCWQKD
Sbjct: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEISLTGKIPSGLFNSMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTK LAFDGVFITLYTVALEKSQMVL D+V+KAVPSSW+PAALARFIETFGTHI+VG+
Sbjct: 121 AANTKTLAFDGVFITLYTVALEKSQMVLLDNVKKAVPSSWEPAALARFIETFGTHIVVGV 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK--- 240
           KMGGKDVIYMKQQHSSTLQ   P+++QK LK+MADKRF +   QYGM SE++Y ND+   
Sbjct: 181 KMGGKDVIYMKQQHSSTLQ---PADIQKKLKDMADKRFLDANGQYGMPSEQVYKNDRFEI 240

Query: 241 --------------------DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPI 300
                               D+V ICKRRGG D  +L H+EWLQTVQ EPDVI+MSFIPI
Sbjct: 241 REQTLRFADTSPSSSYSRKEDVVSICKRRGGIDTRNLSHNEWLQTVQFEPDVIAMSFIPI 300

Query: 301 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNL 360
           TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ + 
Sbjct: 301 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSN 360

Query: 361 ASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTED 420
           ASLQFSLMG KLYVNT+ VDVG+RPVTGLRLYLEGKRSN L+IHLQHLSSLPKIFQL +D
Sbjct: 361 ASLQFSLMGPKLYVNTSLVDVGKRPVTGLRLYLEGKRSNRLAIHLQHLSSLPKIFQLEDD 420

Query: 421 PNGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRL 480
           PNGNFR+DSYDR+Y EKVQ K FSHVCTAPVE+DEDLSIVTGAQLQV N G++N+LFLRL
Sbjct: 421 PNGNFRRDSYDRKYYEKVQWKNFSHVCTAPVESDEDLSIVTGAQLQVENYGIKNILFLRL 480

Query: 481 RFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGG 540
           RFS+V GATVV+  EW+GSPGLA KSGLISTLISHHF+TV KPPP+PADVNINSAVYPGG
Sbjct: 481 RFSTVFGATVVKHPEWDGSPGLAHKSGLISTLISHHFTTVQKPPPRPADVNINSAVYPGG 540

Query: 541 PPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDE 579
           PPVP+QAPKL KFVDTTEM RGPQETPGYWVVSGARL+VEKGRISLRVK+SLLTVILPDE
Sbjct: 541 PPVPIQAPKLRKFVDTTEMTRGPQETPGYWVVSGARLVVEKGRISLRVKYSLLTVILPDE 598

BLAST of Csa3G110020 vs. TrEMBL
Match: W9QEK5_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_001374 PE=4 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 7.7e-275
Identity = 484/607 (79.74%), Postives = 524/607 (86.33%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKG-NLHGSRLIEIDEGVGHEIVLPGGI 60
           MALKV  S AAEIAIGSIGRGYDI+ DLRLKYCKG +   SRLIEIDE  G EIVLPGGI
Sbjct: 1   MALKVPASSAAEIAIGSIGRGYDISTDLRLKYCKGGDSSDSRLIEIDEDDGREIVLPGGI 60

Query: 61  LISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
            ISNV KSIKCDKGERTRFRSDVLSFQQMSEQFNQE++LTGKIPSG FN MF+FSGCWQK
Sbjct: 61  TISNVSKSIKCDKGERTRFRSDVLSFQQMSEQFNQEISLTGKIPSGLFNAMFDFSGCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DAAN K LAFDGVFITLYTVALEKSQMVL DHV+KAVPSSW+PAALARFIETFGTHIIVG
Sbjct: 121 DAANNKTLAFDGVFITLYTVALEKSQMVLRDHVKKAVPSSWEPAALARFIETFGTHIIVG 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK-- 240
           +KMGGKD+IYMKQQHSS LQ   P++VQK LK+MADKRF +   QY   S+++Y NDK  
Sbjct: 181 VKMGGKDIIYMKQQHSSNLQ---PADVQKKLKDMADKRFLDANGQYSSSSQQVYKNDKFE 240

Query: 241 ---------------------DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIP 300
                                DIV ICKRRGGSD  +L HHEWLQTVQ EPDVISMSFIP
Sbjct: 241 IREQQLRFVDTSPSSSYSHKEDIVTICKRRGGSDVRNLSHHEWLQTVQSEPDVISMSFIP 300

Query: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHN 360
           ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ +
Sbjct: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 360

Query: 361 LASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTE 420
            ASLQFS MG KLYVNT PVDVG+RPVTGLRLYLEGKRSN L+IHLQHLSSLPK+FQL +
Sbjct: 361 NASLQFSFMGPKLYVNTIPVDVGKRPVTGLRLYLEGKRSNRLAIHLQHLSSLPKVFQLDD 420

Query: 421 DPNGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLR 480
           DP+GNFR++SYDR+Y EKVQ K FSHVCTAPVE+DEDLSIVTGAQLQV N G++N+LFLR
Sbjct: 421 DPDGNFRRESYDRKYYEKVQWKNFSHVCTAPVESDEDLSIVTGAQLQVENYGIKNILFLR 480

Query: 481 LRFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPG 540
           LRFS V GA VV+  EW+GSPGLA KSGLISTLISHHF+ V KPPP+PADVNINSAVYPG
Sbjct: 481 LRFSRVSGAAVVKHPEWDGSPGLAPKSGLISTLISHHFTMVQKPPPRPADVNINSAVYPG 540

Query: 541 GPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPD 584
           GPPVPVQAPKLLKFVDTTEM RGPQ+ PGYWVVSGARL+VEKGRISLRVK+SLLTVILPD
Sbjct: 541 GPPVPVQAPKLLKFVDTTEMTRGPQDNPGYWVVSGARLVVEKGRISLRVKYSLLTVILPD 600

BLAST of Csa3G110020 vs. TrEMBL
Match: A0A067L1Y7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00244 PE=4 SV=1)

HSP 1 Score: 946.0 bits (2444), Expect = 2.1e-272
Identity = 476/601 (79.20%), Postives = 523/601 (87.02%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MAL++   +AAE AIGSIGRGYDIT DLRLKYCKG+   S LI+IDE  G EIVLPGGI 
Sbjct: 1   MALRLPAPRAAEYAIGSIGRGYDITADLRLKYCKGDAKDSPLIDIDEAGGREIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NV KSIKCDKGERTRFRSDVLSFQQMSEQFNQEM+LTGKIPSG FN+MFEFSGCWQKD
Sbjct: 61  IPNVSKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMSLTGKIPSGLFNSMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTK LAFDGVFITLYTVALEKSQMVL DHV+KAVPSSW+PAALA+FIETFGTHIIVG+
Sbjct: 121 AANTKTLAFDGVFITLYTVALEKSQMVLRDHVKKAVPSSWEPAALAKFIETFGTHIIVGV 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK--- 240
           KMGG+DVIYMKQQHSS LQ   P+++QK LKEMADKRF +T  QYG+G+E++Y N++   
Sbjct: 181 KMGGRDVIYMKQQHSSILQ---PADLQKRLKEMADKRFLDTGGQYGVGAEQVYPNNRLEI 240

Query: 241 --------------------DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPI 300
                               DIV I KRRGGSD   L H +WL TVQ EPDVISMSFIPI
Sbjct: 241 REQRLRFADASSSSSYSHKEDIVKIYKRRGGSDNKVLSHSDWLHTVQFEPDVISMSFIPI 300

Query: 301 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNL 360
           TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQ KQ + 
Sbjct: 301 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQWKQQST 360

Query: 361 ASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTED 420
           ASLQFSLMG KL+VNTTPVDVG+RPVTGLRLYLEGKRSN L+IHLQHLSSLPKIFQL +D
Sbjct: 361 ASLQFSLMGPKLFVNTTPVDVGKRPVTGLRLYLEGKRSNRLAIHLQHLSSLPKIFQLADD 420

Query: 421 PNGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRL 480
           P+GNFRQ+SYDR+Y EKVQ K FSHVCTAPVE DE+LSIVTGAQLQV N G +N+LFLRL
Sbjct: 421 PHGNFRQESYDRKYYEKVQWKNFSHVCTAPVELDEELSIVTGAQLQVENSGFKNILFLRL 480

Query: 481 RFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGG 540
           RFS+VLGAT+V+  EW+GSPG+A KSGLISTLISHHF++V KPPP+PADVNINSAVYPGG
Sbjct: 481 RFSTVLGATIVKHPEWDGSPGMAPKSGLISTLISHHFTSVQKPPPRPADVNINSAVYPGG 540

Query: 541 PPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDE 579
           PPVPVQ PKLLKFVDTTEM RGPQETPGYWVVSGARL+VEKGRISLRV++SLLTVILPDE
Sbjct: 541 PPVPVQVPKLLKFVDTTEMTRGPQETPGYWVVSGARLVVEKGRISLRVRYSLLTVILPDE 598

BLAST of Csa3G110020 vs. TrEMBL
Match: B9SKC8_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0759120 PE=4 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 7.5e-270
Identity = 472/601 (78.54%), Postives = 516/601 (85.86%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALK    +AA+IAIGSIGRGYDIT DLRLKYCKG  +  +LIEID+G G EI+LPGGI 
Sbjct: 1   MALKRPAPEAAKIAIGSIGRGYDITADLRLKYCKGEGNNCQLIEIDDGGGREIILPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I  V KSIKCDKGERTRF SDVLSFQQMSEQFNQEM+LTGKIPSG FN+MFEFSGCWQKD
Sbjct: 61  IPKVSKSIKCDKGERTRFSSDVLSFQQMSEQFNQEMSLTGKIPSGLFNSMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTK LAFDGVFITLYTVALEKSQMVL DHV+KAVPSSW+PAALA+FIETFGTHIIVG+
Sbjct: 121 AANTKTLAFDGVFITLYTVALEKSQMVLRDHVKKAVPSSWEPAALAKFIETFGTHIIVGV 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK--- 240
           KMGGKDV+YMKQQHSS LQ   P++VQK LKEMADKRF +T   YGM SE ++ N+K   
Sbjct: 181 KMGGKDVVYMKQQHSSVLQ---PADVQKRLKEMADKRFLDTGGPYGMASEPVFPNNKLEV 240

Query: 241 --------------------DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPI 300
                               DIV I KRRGG+D   L H EWL TVQ EPDVISMSFIPI
Sbjct: 241 REQRLRFVDTSPSSSYSHKEDIVRIYKRRGGNDDKRLSHSEWLYTVQFEPDVISMSFIPI 300

Query: 301 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNL 360
           TSLLNG+PGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ + 
Sbjct: 301 TSLLNGIPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQST 360

Query: 361 ASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTED 420
           ASLQFSLMG KL+VNTTPVDVG+RPVTGLRLYLEGKRSN L+IHLQHLSSLPK+FQL +D
Sbjct: 361 ASLQFSLMGPKLFVNTTPVDVGKRPVTGLRLYLEGKRSNRLAIHLQHLSSLPKVFQLADD 420

Query: 421 PNGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRL 480
           PNGNF Q+SYDR+Y EKVQ K FSHVCTAPVE+DE+LSIVTG QLQV N G +N+LFLRL
Sbjct: 421 PNGNFNQESYDRKYYEKVQWKNFSHVCTAPVESDEELSIVTGGQLQVENSGFKNILFLRL 480

Query: 481 RFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGG 540
           RFS+V GA  V+  EW+GSPGLA KSGLISTLISHHF++V KPPP+PADVNINSAVYPGG
Sbjct: 481 RFSTVTGAAAVKHPEWDGSPGLAPKSGLISTLISHHFTSVQKPPPRPADVNINSAVYPGG 540

Query: 541 PPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDE 579
           PPVPVQ PKLLKFVDTTEM RGPQETPGYWVVSGARL+VEKGRISLRVK+SLLTV+LPDE
Sbjct: 541 PPVPVQVPKLLKFVDTTEMTRGPQETPGYWVVSGARLVVEKGRISLRVKYSLLTVVLPDE 598

BLAST of Csa3G110020 vs. TAIR10
Match: AT4G24290.2 (AT4G24290.2 MAC/Perforin domain-containing protein)

HSP 1 Score: 859.8 bits (2220), Expect = 1.0e-249
Identity = 431/601 (71.71%), Postives = 496/601 (82.53%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGH-EIVLPGGI 60
           MAL++  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I EG  + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  LISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
            I NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERL------- 240
           +KMGGKDVIY KQQHSS LQP +   +QK LKE+ADKRF E    +  GSER+       
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPED---LQKRLKEVADKRFVEASVVHNTGSERVQASSKVE 240

Query: 241 ----------------YNNDKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIP 300
                           Y N +D V +CKRRGG+D  +L H+EWLQTVQ+EPDVISMSFIP
Sbjct: 241 TKEQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 300

Query: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHN 360
           ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ +
Sbjct: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 360

Query: 361 LASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTE 420
            ASLQFS  G KLYVNTTPVDVG+RP+TG+RLYLEG+RSN L+IHLQHLSSLPKI+QL +
Sbjct: 361 CASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLED 420

Query: 421 DPNGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLR 480
           D N + RQ+S+DRRY EKV  K +SHVCT PVE+D+DLS+VTGAQL V + G +NVLFLR
Sbjct: 421 DLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLR 480

Query: 481 LRFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPG 540
           L FS V+GAT+V+ +EW+ + G A KSGLISTLISHHF+   KPPP+PADVNINSA+YPG
Sbjct: 481 LCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPG 540

Query: 541 GPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPD 578
           GPPVP QAPKLLKFVDT+EM RGPQE+PGYWVVSGARL+VEKG+ISL+VK+SL T IL D
Sbjct: 541 GPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGD 598

BLAST of Csa3G110020 vs. TAIR10
Match: AT1G28380.1 (AT1G28380.1 MAC/Perforin domain-containing protein)

HSP 1 Score: 594.0 bits (1530), Expect = 1.0e-169
Identity = 318/603 (52.74%), Postives = 408/603 (67.66%), Query Frame = 1

Query: 10  AAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSIK 69
           AAE A+  IG GYD+  D+R   CK    GSRL+EID     +++ PGGI+++NV  SIK
Sbjct: 14  AAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK 73

Query: 70  CDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAF 129
           CDKGERTR RSD+LSF QMSE+FNQ+M L+GKIPSG FN MF FS CW KDA++ K LA+
Sbjct: 74  CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAY 133

Query: 130 DGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIY 189
           DG FI+LY+V + + Q+ L D V++ VPSSWD AALA FIE +GTH++VG+ MGGKDVI+
Sbjct: 134 DGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIH 193

Query: 190 MKQQHSSTLQPSEPSEVQKSLKEMADKRF-------------------SETIAQYGM--- 249
           +KQ   S     EP E+QK LK   D+RF                    E + Q+G+   
Sbjct: 194 VKQMRKSN---HEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPF 253

Query: 250 -----GSERLYNNDKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLN 309
                 +  ++  +++I+ +C RRGG D +   H  WL TV   P+VISM F+PITSLL+
Sbjct: 254 GTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITSLLS 313

Query: 310 GVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQF 369
           G+PG+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R + +  SLQF
Sbjct: 314 GLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQF 373

Query: 370 SLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNF 429
           SLMG KLYVNT+ VD G RPVTGLR +LEGK+ N L+IHLQHLS+ P    L+ D     
Sbjct: 374 SLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEP 433

Query: 430 RQDSYDRRYLEKVQSKYFSHVCTAPVE-----ADEDLSIVTGAQLQVVNQGMRNVLFLRL 489
            ++  ++ Y   V+   FSHVCT PV+     +D+  SIVT A L+V   GMR VLFLRL
Sbjct: 434 IEEPVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLFLRL 493

Query: 490 RFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPP---KP-ADVNINSAV 549
            F S+  + V R++ W+     + KSG+ S +IS   ST + P P   KP + ++INSAV
Sbjct: 494 GF-SLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDINSAV 553

Query: 550 YPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVI 577
           YP GP  PV+ PKLL  VDT E+ RGP+E PGYWVV+GA+L VE G+IS++ K+SLLTVI
Sbjct: 554 YPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLLTVI 609

BLAST of Csa3G110020 vs. TAIR10
Match: AT1G29690.1 (AT1G29690.1 MAC/Perforin domain-containing protein)

HSP 1 Score: 459.9 bits (1182), Expect = 2.3e-129
Identity = 273/577 (47.31%), Postives = 353/577 (61.18%), Query Frame = 1

Query: 14  AIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSIKCDKG 73
           AI ++GRG+D+T D+RL YCKG   GSRL+ I+EG   ++ L  G L+ NVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAFDGVF 133
                R  V SF +M+E+FN    + G IP G FN MF ++G WQ DAA+TK+LA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 ITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIYMKQQ 193
           I LY V L K  +VL + +R+AVPSSWDPA+LA FIE +GTHI+  + +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKDIVPICKRRGGSDRIS 253
            SS   P   SE++  + +M   RF E  +Q   G   L   DKDI  I +RRGG D + 
Sbjct: 204 QSS---PLPVSEIENYVNDMIKHRFHEAESQSITGP--LKYKDKDITVIFRRRGGDD-LE 263

Query: 254 LPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEF 313
             H  W +TV   PD+I+M+F PI SLL GVPG   L+ AI LYL YKPPIE+L  FL++
Sbjct: 264 QSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFLDY 323

Query: 314 QLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGK 373
           Q+ R WAP  S L    QRK+   +SLQFSLMG KL+++   V VGR+PVTGLRL LEG 
Sbjct: 324 QIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGS 383

Query: 374 RSNCLSIHLQHLSSLPKIFQLTED---PNGNFR---QDSYDRRYLEKVQSKYFSHVCTAP 433
           + N LSIHLQHL SLPKI Q   D   P G  +    +  D R+ E ++ K FSHV T+P
Sbjct: 384 KQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSP 443

Query: 434 VEADE----DLS---IVTGAQLQVVNQGMRNVLFLRLRFSSVLGATVVRQAEWEGSPGLA 493
           +E  E    DLS   IVTGAQL V + G +NVL L+L FS V G T +R++ W+ +P   
Sbjct: 444 IEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCT-IRRSVWDHTP--V 503

Query: 494 AKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGP-------PVPVQAPKLLKFVDT 553
           A SG +                            PGGP        V  Q+ KL K VD+
Sbjct: 504 ASSGRLE---------------------------PGGPSTSSSTEEVSGQSGKLAKIVDS 559

Query: 554 TEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLL 571
           +EM +GPQ+ PG+W+V+GA+L VEKG+I LRVK+SLL
Sbjct: 564 SEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Csa3G110020 vs. TAIR10
Match: AT1G14780.1 (AT1G14780.1 MAC/Perforin domain-containing protein)

HSP 1 Score: 278.9 bits (712), Expect = 7.3e-75
Identity = 162/364 (44.51%), Postives = 219/364 (60.16%), Query Frame = 1

Query: 234 NNDKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHA 293
           N+   I  IC +RGG  R    H EWL TV  +PD I+ +FIPITSLL  VPGSG LSHA
Sbjct: 263 NSQNGITVICAKRGGDGRAK-SHSEWLITVPDKPDAINFNFIPITSLLKDVPGSGLLSHA 322

Query: 294 INLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS----LQFSLMGSKL 353
           ++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      N+AS    L  + MG KL
Sbjct: 323 MSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFG---AAPNMASAYPALHINFMGPKL 382

Query: 354 YVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIF--QLTEDPNGNFRQDSY 413
           YVNTTPV   + PVTG+R +LEGK+ N L+IHLQHL +       ++T++          
Sbjct: 383 YVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHIWRGSDQIT 442

Query: 414 DR-RYLEKVQSKYFSHVCTAPVEADED--------------LSIVTGAQLQVVNQGMRNV 473
           D  RY E +  K FSHVCT PV+ D +                IVTGAQL+V   G ++V
Sbjct: 443 DNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLEVKKHGSKSV 502

Query: 474 LFLRLRFSSVLGATVVRQAEWEGSPGLAAKSGLISTLI------SHHFSTVMKPPPKPAD 533
           L LRLR++ V    VV+ +   G  G + KSG+ S++       S H + + K      +
Sbjct: 503 LHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHNMIQK---DKNE 562

Query: 534 VNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVK 571
           V ++S V+PGGPPVP    K++KFVD +++ RGPQ +PG+W+V+G RL ++KG++ L VK
Sbjct: 563 VVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDKGKLCLHVK 618

HSP 2 Score: 197.6 bits (501), Expect = 2.1e-50
Identity = 101/213 (47.42%), Postives = 139/213 (65.26%), Query Frame = 1

Query: 12  EIAIGSIGRGYDITIDLRLKYCK---GNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSI 71
           E A+ S+G+G+D+T D RLKYCK   G+    RL+ +D+    E+ +PG  +  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEF-SGCWQKDAANTKNL 131
            CDKGERTRFRSD+L F +MSE FNQ  ++TGKIPSG+FN  F F SG W  DAAN K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 AFDGVFITLYTVALEK-SQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKD 191
             D   +TL+ + +   +++ L D VR AVPSSWDP  LARFIE +GTH+I G+ +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFS 220
           V+ ++Q  SS L   +   ++  L ++ D+ F+
Sbjct: 190 VVVVRQDKSSDL---DNDLLRHHLYDLGDQLFT 219

BLAST of Csa3G110020 vs. NCBI nr
Match: gi|700201121|gb|KGN56254.1| (hypothetical protein Csa_3G110020 [Cucumis sativus])

HSP 1 Score: 1172.5 bits (3032), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKDIV 240
           KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKDIV
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKDIV 240

Query: 241 PICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRY 300
           PICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRY
Sbjct: 241 PICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRY 300

Query: 301 KPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGR 360
           KPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGR
Sbjct: 301 KPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGR 360

Query: 361 RPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFRQDSYDRRYLEKVQSKYF 420
           RPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFRQDSYDRRYLEKVQSKYF
Sbjct: 361 RPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFRQDSYDRRYLEKVQSKYF 420

Query: 421 SHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRFSSVLGATVVRQAEWEGSPGLA 480
           SHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRFSSVLGATVVRQAEWEGSPGLA
Sbjct: 421 SHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRFSSVLGATVVRQAEWEGSPGLA 480

Query: 481 AKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGP 540
           AKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGP
Sbjct: 481 AKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGP 540

Query: 541 QETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEINDN 584
           QETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEINDN
Sbjct: 541 QETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEINDN 583

BLAST of Csa3G110020 vs. NCBI nr
Match: gi|449431854|ref|XP_004133715.1| (PREDICTED: MACPF domain-containing protein At4g24290 [Cucumis sativus])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 583/604 (96.52%), Postives = 583/604 (96.52%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK--- 240
           KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK   
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 ------------------DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300
                             DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS
Sbjct: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420
           LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN
Sbjct: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420

Query: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480
           GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF
Sbjct: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480

Query: 481 SSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540
           SSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP
Sbjct: 481 SSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 584
           VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE
Sbjct: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 600

BLAST of Csa3G110020 vs. NCBI nr
Match: gi|659102661|ref|XP_008452249.1| (PREDICTED: MACPF domain-containing protein At4g24290 [Cucumis melo])

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 579/604 (95.86%), Postives = 581/604 (96.19%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGI+
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVP+SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK--- 240
           KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK   
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 ------------------DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300
                             DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS
Sbjct: 241 REQHLRFGDASTAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420
           LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN
Sbjct: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420

Query: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480
           GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADED SIVTGAQLQVVNQGMRNVLFLRLRF
Sbjct: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDFSIVTGAQLQVVNQGMRNVLFLRLRF 480

Query: 481 SSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540
           SSV GATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP
Sbjct: 481 SSVFGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 584
           VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE
Sbjct: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 600

BLAST of Csa3G110020 vs. NCBI nr
Match: gi|645223629|ref|XP_008218721.1| (PREDICTED: MACPF domain-containing protein At4g24290 [Prunus mume])

HSP 1 Score: 968.0 bits (2501), Expect = 7.4e-279
Identity = 488/601 (81.20%), Postives = 529/601 (88.02%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKV   KAAEIAI SIG GYDI+IDLRLKYCKG  + SRLIEID+  G EIVLPGGI 
Sbjct: 1   MALKVPAPKAAEIAIASIGHGYDISIDLRLKYCKGESYTSRLIEIDDDGGREIVLPGGIT 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQE++LTGKIPSG FN+MFEFSGCWQKD
Sbjct: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEISLTGKIPSGIFNSMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTK LAFDGVFITLYTVALEKSQMVL D+V+KAVPSSW+PAALARFIETFGTHI+VG+
Sbjct: 121 AANTKTLAFDGVFITLYTVALEKSQMVLLDNVKKAVPSSWEPAALARFIETFGTHIVVGV 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK--- 240
           KMGGKDVIYMKQQHSSTLQ   P+++QK LK+MADKRF +   QYGM SE++Y NDK   
Sbjct: 181 KMGGKDVIYMKQQHSSTLQ---PADIQKKLKDMADKRFLDANGQYGMPSEQVYKNDKFEI 240

Query: 241 --------------------DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPI 300
                               DIV ICKRRGGSD  +L H+EWLQTVQ EPDVI+MSFIPI
Sbjct: 241 REQRLHFADTSPSSSYSRKEDIVSICKRRGGSDTRNLSHNEWLQTVQFEPDVIAMSFIPI 300

Query: 301 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNL 360
           TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ + 
Sbjct: 301 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSN 360

Query: 361 ASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTED 420
           ASLQFSLMG KLYVNT+ VDVG+RPVTGLRLYLEGKRSN L+IHLQHLSSLPKIFQL +D
Sbjct: 361 ASLQFSLMGPKLYVNTSLVDVGKRPVTGLRLYLEGKRSNRLAIHLQHLSSLPKIFQLEDD 420

Query: 421 PNGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRL 480
           PNGNFR+DSYDR+Y EKVQ K FSHVCTAPVE+DEDLSIVTGAQLQV N G++N+LFLRL
Sbjct: 421 PNGNFRRDSYDRKYYEKVQWKNFSHVCTAPVESDEDLSIVTGAQLQVENYGIKNILFLRL 480

Query: 481 RFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGG 540
           RFS+V GATVV+  EW+GSPGLA KSGLISTLISHHF+TV KPPP+PADVNINSAVYPGG
Sbjct: 481 RFSTVFGATVVKHPEWDGSPGLAHKSGLISTLISHHFTTVQKPPPRPADVNINSAVYPGG 540

Query: 541 PPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDE 579
           PPVP+QAPKL KFVDTTEM RGPQETPGYWVVSGARL+VEKGRISLRVK+SLLTVILPDE
Sbjct: 541 PPVPIQAPKLRKFVDTTEMTRGPQETPGYWVVSGARLVVEKGRISLRVKYSLLTVILPDE 598

BLAST of Csa3G110020 vs. NCBI nr
Match: gi|1009118432|ref|XP_015875858.1| (PREDICTED: MACPF domain-containing protein At4g24290 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 964.9 bits (2493), Expect = 6.3e-278
Identity = 486/605 (80.33%), Postives = 530/605 (87.60%), Query Frame = 1

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKV   KAAE+AIGSIGRGYDI+ DLRLKYCKG+L GSRLIEID   G +IVLPGGI 
Sbjct: 1   MALKVPALKAAEVAIGSIGRGYDISTDLRLKYCKGDLIGSRLIEIDADDGRDIVLPGGIT 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNV KSIKCDKGERTRFRSDVLSFQQMSEQFNQE+TLTGKIPSG FN MF+FSGCWQKD
Sbjct: 61  ISNVSKSIKCDKGERTRFRSDVLSFQQMSEQFNQEITLTGKIPSGLFNAMFDFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTK LAFDGVFITLYTVALEK+QMVLCDHV+KAVPSSW+PAALARFIETFGTHIIVG+
Sbjct: 121 AANTKTLAFDGVFITLYTVALEKTQMVLCDHVKKAVPSSWEPAALARFIETFGTHIIVGV 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK--- 240
           +MGGKDVIY+KQQHSSTLQ   P++VQK LK+MADKRF +   QY   S+++Y + K   
Sbjct: 181 RMGGKDVIYVKQQHSSTLQ---PADVQKKLKDMADKRFLDANGQYSTSSQQVYKDGKFEI 240

Query: 241 --------------------DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPI 300
                               DIV ICKRRGGSD  +L H+EWL+TVQ EPDVISMSFIPI
Sbjct: 241 REQRLRFADASPSSSYSHKEDIVSICKRRGGSDIRNLSHNEWLRTVQFEPDVISMSFIPI 300

Query: 301 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNL 360
           TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ + 
Sbjct: 301 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSS 360

Query: 361 ASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTED 420
           ASLQFS MG KLYVNTT VDVG+RPVTGLRLYLEGKRSN L+IHLQHLSSLPKIFQL +D
Sbjct: 361 ASLQFSFMGPKLYVNTTSVDVGKRPVTGLRLYLEGKRSNRLAIHLQHLSSLPKIFQLEDD 420

Query: 421 PNGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRL 480
           PNGNFR++SYDR+Y EKVQ K FSHVCTAPVE+DEDLSIVTGAQLQV N G++N+LFLRL
Sbjct: 421 PNGNFRRESYDRKYYEKVQWKNFSHVCTAPVESDEDLSIVTGAQLQVENYGIKNILFLRL 480

Query: 481 RFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGG 540
           RFS+V GA VV+  EW+GSPGLA KSGLISTLISHHF+TV KPPP+PADVNINSAVYPGG
Sbjct: 481 RFSNVSGAAVVKHPEWDGSPGLAHKSGLISTLISHHFTTVQKPPPRPADVNINSAVYPGG 540

Query: 541 PPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDE 583
           PPVPVQAPKLLKFVDTTEM RGPQETPGYWVVSGARL+VEKGRISLRVK+SLLTVILPDE
Sbjct: 541 PPVPVQAPKLLKFVDTTEMTRGPQETPGYWVVSGARLVVEKGRISLRVKYSLLTVILPDE 600