Csa2G357850 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa2G357850
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Probable calcium-binding protein CML18; contains IPR011992 (EF-hand-like domain)
Location	Chr2 : 16888138 .. 16888383 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAACAAGTACAGTTCTTTGAGTGAAGAACAGCTCGCCTGGATTTTCAAGAAACATGACATCGACAGAGACAGGAAGCTGACCAAAGAAGAGTTGACACGAGCCTTCAACTATTTGGGTTCAAGGTGGACCGCCTTGAGAGTCAAGGAAGCCATTCGCGCTGTCGATGCAAACCACGATGGCGTCATCAGTTTGGATGAGATGAGTAAGCTCATTGTGTATGTCAAAGGTTGTCGCACCTAA

mRNA sequence

ATGGGAAACAAGTACAGTTCTTTGAGTGAAGAACAGCTCGCCTGGATTTTCAAGAAACATGACATCGACAGAGACAGGAAGCTGACCAAAGAAGAGTTGACACGAGCCTTCAACTATTTGGGTTCAAGGTGGACCGCCTTGAGAGTCAAGGAAGCCATTCGCGCTGTCGATGCAAACCACGATGGCGTCATCAGTTTGGATGAGATGAGTAAGCTCATTGTGTATGTCAAAGGTTGTCGCACCTAA

Coding sequence (CDS)

ATGGGAAACAAGTACAGTTCTTTGAGTGAAGAACAGCTCGCCTGGATTTTCAAGAAACATGACATCGACAGAGACAGGAAGCTGACCAAAGAAGAGTTGACACGAGCCTTCAACTATTTGGGTTCAAGGTGGACCGCCTTGAGAGTCAAGGAAGCCATTCGCGCTGTCGATGCAAACCACGATGGCGTCATCAGTTTGGATGAGATGAGTAAGCTCATTGTGTATGTCAAAGGTTGTCGCACCTAA

Protein sequence

MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISLDEMSKLIVYVKGCRT*

Homology

[Close]

BLAST of Csa2G357850 vs. TrEMBL
Match: A0A0A0LL93_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357850 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 2.5e-38
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 1


Query: 1  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 60
          MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH
Sbjct: 1  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 60

Query: 61 DGVISLDEMSKLIVYVKGCRT 82
          DGVISLDEMSKLIVYVKGCRT
Sbjct: 61 DGVISLDEMSKLIVYVKGCRT 81

[Close]

BLAST of Csa2G357850 vs. TrEMBL
Match: A0A0A0LRB6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357340 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 1.3e-15
Identity = 46/69 (66.67%), Postives = 52/69 (75.36%), Query Frame = 1


Query: 9  SEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISLDE 68
          SEEQLA +FK HD D D  LTKEEL +AF YLGSR+   RV+EA+RA D N DGVIS+DE
Sbjct: 17 SEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDE 76

Query: 69 MSKLIVYVK 78
          M KLI Y K
Sbjct: 77 MGKLIKYAK 85

[Close]

BLAST of Csa2G357850 vs. TrEMBL
Match: A0A0A0LP43_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357350 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.4e-12
Identity = 40/73 (54.79%), Postives = 52/73 (71.23%), Query Frame = 1


Query: 6  SSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVIS 65
          +SL+E ++A +FK HD D D +LTKEEL  AF YLG ++T  RV +A+ A D N D VIS
Sbjct: 14 ASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVIS 73

Query: 66 LDEMSKLIVYVKG 79
           +EMSKLI+Y  G
Sbjct: 74 FEEMSKLIMYTAG 86

[Close]

BLAST of Csa2G357850 vs. TrEMBL
Match: A0A0D2V374_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G019100 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.9e-10
Identity = 35/70 (50.00%), Postives = 50/70 (71.43%), Query Frame = 1


Query: 7   SLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISL 66
           SL+EEQL  +F+KHD++ D  L+K+EL +AF  LGSR  + RV+ ++   D N DG ISL
Sbjct: 38  SLNEEQLKAVFRKHDVNGDGCLSKDELMKAFRELGSRNPSWRVRRSLHHADGNGDGTISL 97

Query: 67  DEMSKLIVYV 77
           DE+ +L+ YV
Sbjct: 98  DELDELVKYV 107

[Close]

BLAST of Csa2G357850 vs. TrEMBL
Match: A0A067KTQ6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04273 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 4.9e-10
Identity = 36/71 (50.70%), Postives = 46/71 (64.79%), Query Frame = 1


Query: 9   SEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISLDE 68
           SEEQL  +FK +D D DR+L+K+EL   FN LGSR+   RV  A+   DAN DG IS +E
Sbjct: 30  SEEQLMNMFKSYDTDHDRRLSKQELKNLFNKLGSRFAGWRVWRALHHADANKDGYISEEE 89

Query: 69  MSKLIVYVKGC 80
            S L+ Y+  C
Sbjct: 90  FSDLVKYILKC 100

[Close]

BLAST of Csa2G357850 vs. NCBI nr
Match: gi|700207394|gb|KGN62513.1| (hypothetical protein Csa_2G357850 [Cucumis sativus])

HSP 1 Score: 165.6 bits (418), Expect = 3.6e-38
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 1


Query: 1  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 60
          MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH
Sbjct: 1  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 60

Query: 61 DGVISLDEMSKLIVYVKGCRT 82
          DGVISLDEMSKLIVYVKGCRT
Sbjct: 61 DGVISLDEMSKLIVYVKGCRT 81

[Close]

BLAST of Csa2G357850 vs. NCBI nr
Match: gi|659087772|ref|XP_008444628.1| (PREDICTED: probable calcium-binding protein CML18 [Cucumis melo])

HSP 1 Score: 147.5 bits (371), Expect = 1.0e-32
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 1


Query: 1   MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 60
           MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEEL RAF+YLGSRWTA RVK A+RAVDAN 
Sbjct: 35  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELARAFDYLGSRWTARRVKGALRAVDANG 94

Query: 61  DGVISLDEMSKLIVYVKGCRT 82
           DGVIS+DEMSKLIVYVKG RT
Sbjct: 95  DGVISMDEMSKLIVYVKGLRT 115

[Close]

BLAST of Csa2G357850 vs. NCBI nr
Match: gi|700207392|gb|KGN62511.1| (hypothetical protein Csa_2G357340 [Cucumis sativus])

HSP 1 Score: 90.1 bits (222), Expect = 1.9e-15
Identity = 46/69 (66.67%), Postives = 52/69 (75.36%), Query Frame = 1


Query: 9  SEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISLDE 68
          SEEQLA +FK HD D D  LTKEEL +AF YLGSR+   RV+EA+RA D N DGVIS+DE
Sbjct: 17 SEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDE 76

Query: 69 MSKLIVYVK 78
          M KLI Y K
Sbjct: 77 MGKLIKYAK 85

[Close]

BLAST of Csa2G357850 vs. NCBI nr
Match: gi|700207393|gb|KGN62512.1| (hypothetical protein Csa_2G357350 [Cucumis sativus])

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-12
Identity = 40/73 (54.79%), Postives = 52/73 (71.23%), Query Frame = 1


Query: 6  SSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVIS 65
          +SL+E ++A +FK HD D D +LTKEEL  AF YLG ++T  RV +A+ A D N D VIS
Sbjct: 14 ASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVIS 73

Query: 66 LDEMSKLIVYVKG 79
           +EMSKLI+Y  G
Sbjct: 74 FEEMSKLIMYTAG 86

[Close]

BLAST of Csa2G357850 vs. NCBI nr
Match: gi|659087770|ref|XP_008444627.1| (PREDICTED: probable calcium-binding protein CML26 [Cucumis melo])

HSP 1 Score: 75.9 bits (185), Expect = 3.7e-11
Identity = 37/73 (50.68%), Postives = 52/73 (71.23%), Query Frame = 1


Query: 6  SSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVIS 65
          +SL+E ++A +F  +D D D +LTKEEL  AF YLG ++T +RV +A+ A D N D VIS
Sbjct: 14 ASLTEFEVAKVFNNYDRDGDGRLTKEELKSAFKYLGYKFTTIRVHQALHAADNNCDDVIS 73

Query: 66 LDEMSKLIVYVKG 79
           +E+SKLI+Y  G
Sbjct: 74 FEEISKLIIYTAG 86

The following BLAST results are available for this feature:

BLAST of Csa2G357850 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LL93_CUCSA	2.5e-38	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357850 PE=4 SV=1	[more]
A0A0A0LRB6_CUCSA	1.3e-15	66.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357340 PE=4 SV=1	[more]
A0A0A0LP43_CUCSA	1.4e-12	54.79	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357350 PE=4 SV=1	[more]
A0A0D2V374_GOSRA	2.9e-10	50.00	Uncharacterized protein OS=Gossypium raimondii GN=B456_010G019100 PE=4 SV=1	[more]
A0A067KTQ6_JATCU	4.9e-10	50.70	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04273 PE=4 SV=1	[more]

BLAST of Csa2G357850 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207394\|gb\|KGN62513.1\|	3.6e-38	100.00	hypothetical protein Csa_2G357850 [Cucumis sativus]	[more]
gi\|659087772\|ref\|XP_008444628.1\|	1.0e-32	90.12	PREDICTED: probable calcium-binding protein CML18 [Cucumis melo]	[more]
gi\|700207392\|gb\|KGN62511.1\|	1.9e-15	66.67	hypothetical protein Csa_2G357340 [Cucumis sativus]	[more]
gi\|700207393\|gb\|KGN62512.1\|	2.0e-12	54.79	hypothetical protein Csa_2G357350 [Cucumis sativus]	[more]
gi\|659087770\|ref\|XP_008444627.1\|	3.7e-11	50.68	PREDICTED: probable calcium-binding protein CML26 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa2G357850.1	Csa2G357850.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 10..72 score: 1.2
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 12..40 score: 2.0coord: 48..76 score: 0
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 44..79 score: 11.082coord: 8..43 score: 13
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 8..74 score: 1.3
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 7..74 score: 4.13
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 21..33 score: -coord: 57..69 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 4..78 score: 1.7
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 4..78 score: 1.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa2G357850	CmoCh09G002140	Cucurbita moschata (Rifu)	cmocuB002
Csa2G357850	Cp4.1LG07g05240	Cucurbita pepo (Zucchini)	cpecuB819

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa2G357850	Silver-seed gourd	carcuB0202
Csa2G357850	Silver-seed gourd	carcuB0788
Csa2G357850	Watermelon (97103) v2	cuwmbB135
Csa2G357850	Watermelon (97103) v2	cuwmbB163
Csa2G357850	Wax gourd	cuwgoB162
Csa2G357850	Wax gourd	cuwgoB198
Csa2G357850	Cucumber (Chinese Long) v2	cucuB062
Csa2G357850	Cucumber (Chinese Long) v2	cucuB079
Csa2G357850	Cucumber (Gy14) v1	cgycuB318
Csa2G357850	Cucumber (Gy14) v1	cgycuB326
Csa2G357850	Cucurbita maxima (Rimu)	cmacuB011
Csa2G357850	Cucurbita maxima (Rimu)	cmacuB174
Csa2G357850	Cucurbita maxima (Rimu)	cmacuB437
Csa2G357850	Cucurbita maxima (Rimu)	cmacuB784
Csa2G357850	Cucurbita moschata (Rifu)	cmocuB107
Csa2G357850	Cucurbita moschata (Rifu)	cmocuB158
Csa2G357850	Cucurbita moschata (Rifu)	cmocuB424
Csa2G357850	Cucurbita moschata (Rifu)	cmocuB777
Csa2G357850	Melon (DHL92) v3.5.1	cumeB106
Csa2G357850	Melon (DHL92) v3.5.1	cumeB117
Csa2G357850	Melon (DHL92) v3.5.1	cumeB149
Csa2G357850	Watermelon (Charleston Gray)	cuwcgB140
Csa2G357850	Watermelon (Charleston Gray)	cuwcgB169
Csa2G357850	Watermelon (97103) v1	cuwmB111
Csa2G357850	Watermelon (97103) v1	cuwmB126
Csa2G357850	Cucurbita pepo (Zucchini)	cpecuB096
Csa2G357850	Cucurbita pepo (Zucchini)	cpecuB539
Csa2G357850	Cucurbita pepo (Zucchini)	cpecuB771
Csa2G357850	Bottle gourd (USVL1VR-Ls)	culsiB117
Csa2G357850	Bottle gourd (USVL1VR-Ls)	culsiB142
Csa2G357850	Cucumber (Gy14) v2	cgybcuB057
Csa2G357850	Cucumber (Gy14) v2	cgybcuB160
Csa2G357850	Cucumber (Gy14) v2	cgybcuB297
Csa2G357850	Melon (DHL92) v3.6.1	cumedB100
Csa2G357850	Melon (DHL92) v3.6.1	cumedB114
Csa2G357850	Melon (DHL92) v3.6.1	cumedB141