Csa2G299920 (gene) Cucumber (Chinese Long) v2

NameCsa2G299920
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
Description(R)-limonene synthase, putative; contains IPR008930 (Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid), IPR008949 (Terpenoid synthase)
LocationChr2 : 14372485 .. 14375724 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTTTTGTGTTTCAATATTGATCAGCTAAGCTTGATTACACGTTTTTTGGTGTTCTCTATCAATGGCTCATCTTCATCAAATTTTCACTTCTTTCTTCCCTGTGAGTTCTCTTGTGATGGAACGAGTCTCTTCTTTTAATCACAACTCTTTGTTTGTCCCTCGTGTTATAAATAATGTAACTGGTGGTGGTGCAAGAATGTGTAGCCAGACTGTTGTACGTCGATCCGGAAATTACCAACCTTCTACTTGGAAATACGAGTTCATTCAATCATTAAGCAGTGAATTTGAGGTATGGAGATGGTAGAAAATTTTAAACTCAACCAAACTTTTTAAAATATTTATAAATATAACAAAATTATATAACAATTTATCGACGACAATAGATTAAGATAGACTAATATTTATGTCACCTATTGCGATCCATCGTAAATAAACTGAGATTTTTAAAAGTTTTGTAAATATTTTGGTTCATTTTGCTATATTTGAAACTAACTCATTTATTTACCGTGGGTTTGATAACAATTATTATGATTATTTTTTTGGTTTTGAGAATTTTTATGTTTGGATTTTGACCTTTTATTCAAGAAATAATGTAGTTTTAATTTAATGTTAGTTTGTTTTAAAACAAATTAAATTAGGATGGTTTTTGAAACATTAGTAGAAAATAGGTACCAAAAACACAACAATTTGGAGGTGAAACTAAATTAAAATGGTTTATCAAACTATGCATTTTAATAAATACTAAATTTTGAAAATAATTGAGAATAACTTGTGGACATGCACGTTGTTGTGTGTAGGGAGAAATATATGTAGGACTGTTTAATGAGTTAAAGGGAGAGGTAAGAGTCATCATGAACAAAATAGCTAATGACGATCCTCTAAAGCAACTCGAGTTTATTGATATCTTACAACGACTTGGCATCTCATACCATTTTGAGAATGAGATTAAAGATATGTTGACAATGACATACAAGAATCACTGTGAAAATGACGATTGGAAAATCAATAATCTCTACGCAACATCTCTTGAGTTTAGACTTCTAAGACAATATGGATTCAATATTCAACAAGGTACGACTTATTAATATTAATTGTATTACCATGCATATCAATATGAGCGTTGGAGAATCGAACTCTCACCTCCATGTTGTTGATATGTTGAACATGTCACTTCATCTCTTAAATTGATGTCTAATAAATTCAACAAGTGAAAATTATTCGATAGTCCTGTGATGTTGCATTATATTTTAATTTATATTATTTAACAAAAAAAAACTTTTATATTAATGTCAGATGTTTTCAATCACTTCTTCTCGAATGAGACGAAAAGTTTTAACGTGCAACTGTACGAGGATTTAAATGGAATGCTATGTTTGTACGAAGCTTCATTCTTAAGTATAGAAGGCGAAAACATTTTAGAGACGGCAAAACATTTTACGATGGAATATTTAGAAAAATACATGAAATCAAGCACGGATGAGAATGAAGTAGCCATTGTTAGACATGCATTGGAGCTTCCCTTACATTGGAGAATGCCAAGATTGGAGACACGATGGTTCATTGATATCTACGAGAGAAAAGTGGATATGAATCCTATTCTTCTTGAATTTGCTAAACTTGATTTCAATAAGGTGCAATTTATCCATCAACAAGATTTAAAATATGCATCAAGGTGATACTAAATTTATCATTCAGCCAATATCAAAATATACATACATATACTTGAGTAATAATTTAGACATAAGATCCATGTTAAATCGTGAATCAACTTATAATTAAGTACTTTAAATATGTGCCACAGTAAAATTTATCAACTTATCATTGCATTATATTGCCATGGATAGGTGGTGGAAGAGTAGTGGGCTTGGACAAAAGTTGAGCTTTGCCAGAGATAGATTGATAGAGAGTTTCTTTTGGACTGTGGGTATTGGATTTGAACCTGAGTTCTCTTATTTTAGAAGATCGTTAACAAAAATCAATGCATTAATAACAATCATAGATGATGTGTATGATGTTTATGGCTCATTAGATGAGCTCGAACTCTTTACCAACGCAGTAGAAAGGTAAGTTGTAATATCCTAAACTTCATATTATTTCATACGAATACTTAAATAGTTTTATTAATATATGTATTTTTTTTCTTCTTTGTTAAGAAAAAAATATTGTATCAATTTTGTTTGCATAACCAAGTTCAGTTATATAGCTTAAATTTTTTGTTGCCACTTTAGTTTATAACAATTTAATGTTTAAATGATGTTAATACATAATTTAGAGTCTATTAACTATTTGATTTTTGTTTAAAATTGTGATTATTTAGGTGGGATGTTGCAGCAATGGAAGAGCTTCCTGACTATATGAAAATATGTTTTCTTGCTCTTCACAACTCTATAAATGAGATGGGTTTCGAAGCACTAAGAGACAAAGGAATTAATGTCATTCAATACCTTAAGAAAGCAGTATGAATTTATTTTAACATATGCAAAACTTTTTAAACTTTAGGGATTTGGTTTACATCAGTCAATGATATTAATCATTATGGTCTATTCTAAATTTTGCAGTGGGTAGATTTGTGCAAAAGTTATATGTTGGAGGCAAAGTGGTACCACACCAATTATAAACCAACATTGGAAGAATATTTAGATAATGCATGGATTTCAATTTCAGGAACAGTTTTATTAGTTCATACATATGTTTTTGTCACATCTTCCACGCTTGAAAATATGGAAAGTTTGAAACAGCACATTGATATGATTCGTTGGTCATCAACAATTTTACGACTTGCTGATGATCTAGGAACATCATCGGTACGTGAACTATTAGACTTGAATAATCATGAATCTTGACATAAAATGTTAACTCATTATTGTTATTTTATTATATGACTAACTAGTGTTGTGATGGAAAAATATAATTTCATTTAGGATGAATTAGAAAGAGGTGATGTTCCAAAATCAATACAATGTTACATGAACGACACGGGAGCTTCAGAAAGTAATGCACGGGAATATATAAGACATTTGATTGATGAGACGTGGAAGAAGTTAAATAAGACAGAAGTCGAGAATAATTATTCCATTTTTCCTCGAGTGTTCGTTGAAAGAGCTAAGAATCTTGCTAGAATGGCTCAATGCATGTATCAATATGGTGATGGACATGGCATGGGATACCAAGAAACAAAAGATCGTGTAATGTCTCTACTTATTGAACCGATTTCCATTCATTGTTCATATAGTGAATAA

mRNA sequence

ATGGCTCATCTTCATCAAATTTTCACTTCTTTCTTCCCTGTGAGTTCTCTTGTGATGGAACGAGTCTCTTCTTTTAATCACAACTCTTTGTTTGTCCCTCGTGTTATAAATAATGTAACTGGTGGTGGTGCAAGAATGTGTAGCCAGACTGTTGTACGTCGATCCGGAAATTACCAACCTTCTACTTGGAAATACGAGTTCATTCAATCATTAAGCAGTGAATTTGAGGGAGAAATATATGTAGGACTGTTTAATGAGTTAAAGGGAGAGGTAAGAGTCATCATGAACAAAATAGCTAATGACGATCCTCTAAAGCAACTCGAGTTTATTGATATCTTACAACGACTTGGCATCTCATACCATTTTGAGAATGAGATTAAAGATATGTTGACAATGACATACAAGAATCACTGTGAAAATGACGATTGGAAAATCAATAATCTCTACGCAACATCTCTTGAGTTTAGACTTCTAAGACAATATGGATTCAATATTCAACAAGATGTTTTCAATCACTTCTTCTCGAATGAGACGAAAAGTTTTAACGTGCAACTGTACGAGGATTTAAATGGAATGCTATGTTTGTACGAAGCTTCATTCTTAAGTATAGAAGGCGAAAACATTTTAGAGACGGCAAAACATTTTACGATGGAATATTTAGAAAAATACATGAAATCAAGCACGGATGAGAATGAAGTAGCCATTGTTAGACATGCATTGGAGCTTCCCTTACATTGGAGAATGCCAAGATTGGAGACACGATGGTTCATTGATATCTACGAGAGAAAAGTGGATATGAATCCTATTCTTCTTGAATTTGCTAAACTTGATTTCAATAAGGTGCAATTTATCCATCAACAAGATTTAAAATATGCATCAAGGTGGTGGAAGAGTAGTGGGCTTGGACAAAAGTTGAGCTTTGCCAGAGATAGATTGATAGAGAGTTTCTTTTGGACTGTGGGTATTGGATTTGAACCTGAGTTCTCTTATTTTAGAAGATCGTTAACAAAAATCAATGCATTAATAACAATCATAGATGATGTGTATGATGTTTATGGCTCATTAGATGAGCTCGAACTCTTTACCAACGCAGTAGAAAGGGATTTGGATGAATTAGAAAGAGGTGATGTTCCAAAATCAATACAATGTTACATGAACGACACGGGAGCTTCAGAAAGTAATGCACGGGAATATATAAGACATTTGATTGATGAGACGTGGAAGAAGTTAAATAAGACAGAAGTCGAGAATAATTATTCCATTTTTCCTCGAGTGTTCGTTGAAAGAGCTAAGAATCTTGCTAGAATGGCTCAATGCATGTATCAATATGGTGATGGACATGGCATGGGATACCAAGAAACAAAAGATCGTGTAATGTCTCTACTTATTGAACCGATTTCCATTCATTGTTCATATAGTGAATAA

Coding sequence (CDS)

ATGGCTCATCTTCATCAAATTTTCACTTCTTTCTTCCCTGTGAGTTCTCTTGTGATGGAACGAGTCTCTTCTTTTAATCACAACTCTTTGTTTGTCCCTCGTGTTATAAATAATGTAACTGGTGGTGGTGCAAGAATGTGTAGCCAGACTGTTGTACGTCGATCCGGAAATTACCAACCTTCTACTTGGAAATACGAGTTCATTCAATCATTAAGCAGTGAATTTGAGGGAGAAATATATGTAGGACTGTTTAATGAGTTAAAGGGAGAGGTAAGAGTCATCATGAACAAAATAGCTAATGACGATCCTCTAAAGCAACTCGAGTTTATTGATATCTTACAACGACTTGGCATCTCATACCATTTTGAGAATGAGATTAAAGATATGTTGACAATGACATACAAGAATCACTGTGAAAATGACGATTGGAAAATCAATAATCTCTACGCAACATCTCTTGAGTTTAGACTTCTAAGACAATATGGATTCAATATTCAACAAGATGTTTTCAATCACTTCTTCTCGAATGAGACGAAAAGTTTTAACGTGCAACTGTACGAGGATTTAAATGGAATGCTATGTTTGTACGAAGCTTCATTCTTAAGTATAGAAGGCGAAAACATTTTAGAGACGGCAAAACATTTTACGATGGAATATTTAGAAAAATACATGAAATCAAGCACGGATGAGAATGAAGTAGCCATTGTTAGACATGCATTGGAGCTTCCCTTACATTGGAGAATGCCAAGATTGGAGACACGATGGTTCATTGATATCTACGAGAGAAAAGTGGATATGAATCCTATTCTTCTTGAATTTGCTAAACTTGATTTCAATAAGGTGCAATTTATCCATCAACAAGATTTAAAATATGCATCAAGGTGGTGGAAGAGTAGTGGGCTTGGACAAAAGTTGAGCTTTGCCAGAGATAGATTGATAGAGAGTTTCTTTTGGACTGTGGGTATTGGATTTGAACCTGAGTTCTCTTATTTTAGAAGATCGTTAACAAAAATCAATGCATTAATAACAATCATAGATGATGTGTATGATGTTTATGGCTCATTAGATGAGCTCGAACTCTTTACCAACGCAGTAGAAAGGGATTTGGATGAATTAGAAAGAGGTGATGTTCCAAAATCAATACAATGTTACATGAACGACACGGGAGCTTCAGAAAGTAATGCACGGGAATATATAAGACATTTGATTGATGAGACGTGGAAGAAGTTAAATAAGACAGAAGTCGAGAATAATTATTCCATTTTTCCTCGAGTGTTCGTTGAAAGAGCTAAGAATCTTGCTAGAATGGCTCAATGCATGTATCAATATGGTGATGGACATGGCATGGGATACCAAGAAACAAAAGATCGTGTAATGTCTCTACTTATTGAACCGATTTCCATTCATTGTTCATATAGTGAATAA

Protein sequence

MAHLHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISYHFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKSFNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELELFTNAVERDLDELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVERAKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE*
BLAST of Csa2G299920 vs. Swiss-Prot
Match: ATESY_VITVI ((-)-alpha-terpineol synthase OS=Vitis vinifera PE=1 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 4.2e-107
Identity = 192/380 (50.53%), Postives = 275/380 (72.37%), Query Frame = 1

Query: 43  GARMCSQTVVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDD 102
           G   C + +VRR+ NY P+ W Y+++QSL S++ GE Y    ++LK +V+ ++ K+    
Sbjct: 39  GNSNCEEIIVRRTANYHPTIWDYDYVQSLRSDYVGETYTRRLDKLKRDVKPMLGKVKK-- 98

Query: 103 PLKQLEFIDILQRLGISYHFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYG 162
           PL QLE ID+LQRLGI YHF++EIK +L   Y  +  +++W+ ++LYAT+LEFRLLRQ+G
Sbjct: 99  PLDQLELIDVLQRLGIYYHFKDEIKRILNGIYNQYNRHEEWQKDDLYATALEFRLLRQHG 158

Query: 163 FNIQQDVFNHFFSNETKSFNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEK 222
           +++ QDVF+ F  ++T SF   L ED+ GMLCLYEAS+L ++GE+ +E A+ F   +L K
Sbjct: 159 YDVPQDVFSRF-KDDTGSFKACLCEDMKGMLCLYEASYLCVQGESTMEQARDFAHRHLGK 218

Query: 223 YMKSSTDENEVAIVRHALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQ 282
            ++ + D+N    V+HALELPLHWRMPRLE RWFID+YE++ DMNPILLEFAKLDFN VQ
Sbjct: 219 GLEQNIDQNLAIEVKHALELPLHWRMPRLEARWFIDVYEKRQDMNPILLEFAKLDFNMVQ 278

Query: 283 FIHQQDLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALI 342
             HQ+DL++ S WW S+ LG+KL+FARDRL+E+F WTVG+ FEP++ Y RR  TK+N LI
Sbjct: 279 ATHQEDLRHMSSWWSSTRLGEKLNFARDRLMENFLWTVGVIFEPQYGYCRRMSTKVNTLI 338

Query: 343 TIIDDVYDVYGSLDELELFTNAVER-DLDELERGDVPKSIQ-CYM---NDTGASESNA-R 402
           TIIDDVYDVYG++DELELFT+ V+R D++ ++   +P+ ++ C++   N T     +A +
Sbjct: 339 TIIDDVYDVYGTMDELELFTDVVDRWDINAMD--PLPEYMKLCFLALYNSTNEMAYDALK 398

Query: 403 EYIRHLID---ETWKKLNKT 414
           E+  H++    + W  L K+
Sbjct: 399 EHGLHIVSYLRKAWSDLCKS 413


HSP 2 Score: 132.5 bits (332), Expect = 1.2e-29
Identity = 65/98 (66.33%), Postives = 76/98 (77.55%), Query Frame = 1

Query: 369 LDELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVE 428
           LDEL+RGDVPKSIQCYM +TGASE +AR++  +LI ETWKKLN+       S FP  F+ 
Sbjct: 489 LDELKRGDVPKSIQCYMYETGASEEDARKHTSYLIGETWKKLNEDGAVE--SPFPETFIG 548

Query: 429 RAKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPI 467
            A NLARMAQCMYQ+GDGHG+ Y ET+DRV+SLL+EPI
Sbjct: 549 IAMNLARMAQCMYQHGDGHGIEYGETEDRVLSLLVEPI 584

BLAST of Csa2G299920 vs. Swiss-Prot
Match: MYRS_QUEIL (Myrcene synthase, chloroplastic OS=Quercus ilex PE=1 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 2.7e-106
Identity = 191/328 (58.23%), Postives = 244/328 (74.39%), Query Frame = 1

Query: 48  SQTVVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQL 107
           S  ++RRS NYQPS W +++I+SL  E+ GE      N LK +VR++++K+ N  PL+QL
Sbjct: 52  SPDILRRSANYQPSIWNHDYIESLRIEYVGETCTRQINVLKEQVRMMLHKVVN--PLEQL 111

Query: 108 EFIDILQRLGISYHFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQ 167
           E I+ILQRLG+SYHFE EIK +L   Y N    D WK  NLYAT+L+FRLLRQ+G+++ Q
Sbjct: 112 ELIEILQRLGLSYHFEEEIKRILDGVYNNDHGGDTWKAENLYATALKFRLLRQHGYSVSQ 171

Query: 168 DVFNHFFSNETKSFNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSS 227
           +VFN F  +E  SF   L ED  GML LYEASF  IEGENILE A+ F+ ++LE+Y+K +
Sbjct: 172 EVFNSF-KDERGSFKACLCEDTKGMLSLYEASFFLIEGENILEEARDFSTKHLEEYVKQN 231

Query: 228 TDENEVAIVRHALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQ 287
            ++N   +V H+LE PLHWRMPRLE RWFI+IY    D+NPILLEFA+LDFN VQ  HQ 
Sbjct: 232 KEKNLATLVNHSLEFPLHWRMPRLEARWFINIYRHNQDVNPILLEFAELDFNIVQAAHQA 291

Query: 288 DLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDD 347
           DLK  S WWKS+GL + LSFARDR +E+FFWTVG+ F+P+F Y RR  TK+ ALIT IDD
Sbjct: 292 DLKQVSTWWKSTGLVENLSFARDRPVENFFWTVGLIFQPQFGYCRRMFTKVFALITTIDD 351

Query: 348 VYDVYGSLDELELFTNAVER-DLDELER 375
           VYDVYG+LDELELFT+ VER D++ +++
Sbjct: 352 VYDVYGTLDELELFTDVVERWDINAMDQ 376


HSP 2 Score: 141.0 bits (354), Expect = 3.4e-32
Identity = 65/100 (65.00%), Postives = 81/100 (81.00%), Query Frame = 1

Query: 370 DELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVER 429
           DEL+RGDVPK+IQCYMN+TGASE  AREYI++LI  TWKK+NK    +  S F  +F+E 
Sbjct: 498 DELKRGDVPKAIQCYMNETGASEEGAREYIKYLISATWKKMNKDRAAS--SPFSHIFIEI 557

Query: 430 AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIH 470
           A NLARMAQC+YQ+GDGHG+G +ETKDR++SLLI+PI ++
Sbjct: 558 ALNLARMAQCLYQHGDGHGLGNRETKDRILSLLIQPIPLN 595

BLAST of Csa2G299920 vs. Swiss-Prot
Match: TPS10_RICCO (Terpene synthase 10 OS=Ricinus communis GN=TPS10 PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.2e-104
Identity = 179/322 (55.59%), Postives = 244/322 (75.78%), Query Frame = 1

Query: 46  MCSQTVVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLK 105
           M ++T++RRS NY+P  W ++F+QSL SEF GE+ +   ++LK +V++++NK     P  
Sbjct: 6   MSNETIIRRSANYRPPIWDFDFVQSLKSEFVGELNIKRIDKLKEDVKMMLNKTMA--PSD 65

Query: 106 QLEFIDILQRLGISYHFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNI 165
           Q E ID LQRLG++YHF +EIK ++   Y +H  ++ W   +L+  +L+FRLLRQ+G+NI
Sbjct: 66  QFELIDTLQRLGLAYHFGDEIKRIVKSIYNSHRNDNTWMKEDLHTIALQFRLLRQHGYNI 125

Query: 166 QQDVFNHFFSNETKSFNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMK 225
            Q++F+  F +E  +F   L+ED+ GML LYEAS+L  EGENILE A+ F    L+KY++
Sbjct: 126 SQEIFD-IFRDELGNFKECLHEDIEGMLSLYEASYLLEEGENILEVAREFAASCLKKYIQ 185

Query: 226 SSTDENEVAIVRHALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIH 285
            + D+    IV H+LE+PLHWRMPRLETRWFIDIYE+K  MNP+LLE AKLDFN VQ  +
Sbjct: 186 VNKDQLLSMIVSHSLEVPLHWRMPRLETRWFIDIYEKKQGMNPLLLELAKLDFNNVQATY 245

Query: 286 QQDLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITII 345
            +DLKY + WW+++GLG+KLSFARDRL+E+F WTVG+ F P+F YFRR  TK+N+LIT+I
Sbjct: 246 HEDLKYVTSWWRNTGLGEKLSFARDRLMENFLWTVGVNFPPQFGYFRRISTKVNSLITVI 305

Query: 346 DDVYDVYGSLDELELFTNAVER 368
           DD+YDVYG+LDEL+LFTNAVER
Sbjct: 306 DDIYDVYGTLDELQLFTNAVER 324


HSP 2 Score: 128.3 bits (321), Expect = 2.3e-28
Identity = 68/138 (49.28%), Postives = 89/138 (64.49%), Query Frame = 1

Query: 329 SYFRRSLTKINALITIIDDVYDVYGSLDELELFTNAVERDLDELERGDVPKSIQCYMNDT 388
           +YF       N  +  + +  D+      +  F + +    DEL+RGD PKSIQCY+ +T
Sbjct: 413 AYFLEGSPITNEALKSLKEYPDIIQWSSMILRFADDLGTSSDELKRGDNPKSIQCYIYET 472

Query: 389 GASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVERAKNLARMAQCMYQYGDGHG 448
           G SE  ARE+I++LI ETWKK+NK E E   S F ++F+E A NLARMAQCMYQ+GDGHG
Sbjct: 473 GVSELKAREHIQYLIGETWKKINK-EREYIDSPFSKIFIEVATNLARMAQCMYQHGDGHG 532

Query: 449 MGYQETKDRVMSLLIEPI 467
           +   ETKD V+SLL++PI
Sbjct: 533 IEDGETKDHVLSLLVKPI 549

BLAST of Csa2G299920 vs. Swiss-Prot
Match: TPS2_CANSA ((+)-alpha-pinene synthase, chloroplastic OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.5e-91
Identity = 190/385 (49.35%), Postives = 252/385 (65.45%), Query Frame = 1

Query: 11  FFPVSSLVMERVSSFNHN----SLFVPRVINNVTGGGAR--------MCS---------Q 70
           F P SSL +   ++ N++     +F P+  +N+T   +R         CS          
Sbjct: 9   FAPSSSLSIFSSTNINNHFFGREIFTPKT-SNITTKKSRSRPNCNPIQCSLAKSPSSDTS 68

Query: 71  TVVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEF 130
           T+VRRS NY P  W ++FIQSL  +++GE Y    N+LK EV+ ++  +  ++ L QLE 
Sbjct: 69  TIVRRSANYDPPIWSFDFIQSLPCKYKGEPYTSRSNKLKEEVKKML--VGMENSLVQLEL 128

Query: 131 IDILQRLGISYHFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDV 190
           ID LQRLGISYHFENEI  +L   + N   N + K + LYAT+LEFRLLR+YG+ I Q++
Sbjct: 129 IDTLQRLGISYHFENEIISILKEYFTNISTNKNPKYD-LYATALEFRLLREYGYAIPQEI 188

Query: 191 FNHFFSNETKSFNVQLY-EDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSST 250
           FN F  +ET  F   +  +D+ G+L LYEASF    GENILE A+ FT EYL++Y+    
Sbjct: 189 FNDF-KDETGKFKASIKNDDIKGVLALYEASFYVKNGENILEEARVFTTEYLKRYVMMID 248

Query: 251 -----DENEVAIVRHALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQF 310
                ++N   +VRHALE+PLHWR  R E +WFI+ YE+  D N  LLEFAKLDFN +Q 
Sbjct: 249 QNIILNDNMAILVRHALEMPLHWRTIRAEAKWFIEEYEKTQDKNGTLLEFAKLDFNMLQS 308

Query: 311 IHQQDLKYASRWWKSSGLGQ-KLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALI 368
           I Q+DLK+ SRWW+ S LG+ K+ +ARDRL+E+F W VG+ FEP+FS+FRR   +I ALI
Sbjct: 309 IFQEDLKHVSRWWEHSELGKNKMVYARDRLVEAFLWQVGVRFEPQFSHFRRISARIYALI 368


HSP 2 Score: 110.5 bits (275), Expect = 5.0e-23
Identity = 53/99 (53.54%), Postives = 68/99 (68.69%), Query Frame = 1

Query: 370 DELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVER 429
           DE++RGDVPKSIQCYM+DTGASE  ARE+I+ LI E WK++N  +  N  SIF + FV+ 
Sbjct: 519 DEMKRGDVPKSIQCYMHDTGASEDEAREHIKFLISEIWKEMNDEDEYN--SIFSKEFVQA 578

Query: 430 AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISI 469
            KNL RM+  MYQ+GDGH      ++ R+  L+I PI +
Sbjct: 579 CKNLGRMSLFMYQHGDGHASQDSHSRKRISDLIINPIPL 615

BLAST of Csa2G299920 vs. Swiss-Prot
Match: RLC1_CITLI ((R)-limonene synthase 1 OS=Citrus limon PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 7.3e-91
Identity = 170/327 (51.99%), Postives = 237/327 (72.48%), Query Frame = 1

Query: 44  ARMCSQTVVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDP 103
           A+  + TV RRS NYQPS W ++F+QSL+S +  E Y     EL+G+V++ +  +   +P
Sbjct: 44  AKYDNLTVDRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVI--EP 103

Query: 104 LKQLEFIDILQRLGISYHFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGF 163
           L QLE ID LQRLG+++ FE EI+++L   Y N+ ++ +W+  NLYATSLEFRLLRQ+G+
Sbjct: 104 LDQLELIDNLQRLGLAHRFETEIRNILNNIYNNN-KDYNWRKENLYATSLEFRLLRQHGY 163

Query: 164 NIQQDVFNHFFSNETKSFNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKY 223
            + Q+VFN F  ++   F   + +D  G+L L+EAS+ S+EGE+I+E A  FT ++L++ 
Sbjct: 164 PVSQEVFNGF-KDDQGGF---ICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEV 223

Query: 224 MKSSTDENEVAIV---RHALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNK 283
           M S   E +V +    + ALELPLHW++P LE RWFI IYER+ D N +LLE AK++FN 
Sbjct: 224 MISKNMEEDVFVAEQAKRALELPLHWKVPMLEARWFIHIYERREDKNHLLLELAKMEFNT 283

Query: 284 VQFIHQQDLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINA 343
           +Q I+Q++LK  S WWK +GLG+KLSFAR+RL+ SF W++GI FEP+F+Y RR LT   A
Sbjct: 284 LQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIA 343

Query: 344 LITIIDDVYDVYGSLDELELFTNAVER 368
           LIT+IDD+YDVYG+LDELE+FT+AVER
Sbjct: 344 LITVIDDIYDVYGTLDELEIFTDAVER 363


HSP 2 Score: 93.6 bits (231), Expect = 6.3e-18
Identity = 47/99 (47.47%), Postives = 64/99 (64.65%), Query Frame = 1

Query: 370 DELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVER 429
           DE++RGDVPKSIQCYM++TGASE  AR++I+ ++ + WKK+N    + + S       E 
Sbjct: 494 DEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKD-SPLTGTTTEF 553

Query: 430 AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISI 469
             NL RM+  MY +GDGHG+  QET D   +LL +PI +
Sbjct: 554 LLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPL 591

BLAST of Csa2G299920 vs. TrEMBL
Match: A0A0A0LMX2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299920 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 5.5e-279
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 1

Query: 1   MAHLHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQP 60
           MAHLHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQP
Sbjct: 1   MAHLHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQP 60

Query: 61  STWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISY 120
           STWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISY
Sbjct: 61  STWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISY 120

Query: 121 HFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKS 180
           HFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKS
Sbjct: 121 HFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKS 180

Query: 181 FNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHAL 240
           FNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHAL
Sbjct: 181 FNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHAL 240

Query: 241 ELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSG 300
           ELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSG
Sbjct: 241 ELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSG 300

Query: 301 LGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELEL 360
           LGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELEL
Sbjct: 301 LGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELEL 360

Query: 361 FTNAVERDLDELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYS 420
           FTNAVERDLDELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYS
Sbjct: 361 FTNAVERDLDELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYS 420

Query: 421 IFPRVFVERAKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE 475
           IFPRVFVERAKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE
Sbjct: 421 IFPRVFVERAKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE 474

BLAST of Csa2G299920 vs. TrEMBL
Match: A0A0A0LM62_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299890 PE=3 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 6.8e-120
Identity = 225/292 (77.05%), Postives = 251/292 (85.96%), Query Frame = 1

Query: 4   LHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGA-RMCSQTVVRRSGNYQPST 63
           LHQ  TS    +S  +ERVS  N N  F+PR++  ++   A RMCSQT+VRRSGNYQP  
Sbjct: 3   LHQFPTS--SQTSRFIERVSC-NFNPSFIPRIVKVISKTTATRMCSQTIVRRSGNYQPPF 62

Query: 64  WKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISYHF 123
           WK+EFIQSL SEF GEIYVG FNELK E+R+I+N+I  DDPLKQLE ID LQRLGISYHF
Sbjct: 63  WKHEFIQSLRSEFGGEIYVGRFNELKREIRLIINQII-DDPLKQLELIDTLQRLGISYHF 122

Query: 124 ENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKSFN 183
           ENEIK++L  TY+   END WK NNLYATSLEFRLLRQ+GFN+ QDVFN+F+S+ETKSFN
Sbjct: 123 ENEIKNVLKTTYEKSYENDYWKNNNLYATSLEFRLLRQHGFNLSQDVFNNFYSSETKSFN 182

Query: 184 VQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHALEL 243
            Q+Y+DLNGML LYEASFLSIEGENILETAKHFT++YLEKYMKSS DENEVAIVRHALEL
Sbjct: 183 TQMYQDLNGMLFLYEASFLSIEGENILETAKHFTVKYLEKYMKSSKDENEVAIVRHALEL 242

Query: 244 PLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASR 295
           PLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFN+VQ IHQQDLKYASR
Sbjct: 243 PLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNRVQSIHQQDLKYASR 290

BLAST of Csa2G299920 vs. TrEMBL
Match: A0A0A0LM58_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299350 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.8e-112
Identity = 203/249 (81.53%), Postives = 223/249 (89.56%), Query Frame = 1

Query: 46  MCSQTVVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLK 105
           MCS+TV+RRSGNYQPS WK+EFIQSL SEF  EIY+  FN+LKGE+RVIMN I  DDPLK
Sbjct: 1   MCSKTVIRRSGNYQPSIWKHEFIQSLRSEFAEEIYMRRFNQLKGEIRVIMNTII-DDPLK 60

Query: 106 QLEFIDILQRLGISYHFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNI 165
           QLE ID+LQRLGISYHFENEIK++L   Y    E + WK NNLYATSLEFRLLRQ+GFN+
Sbjct: 61  QLELIDMLQRLGISYHFENEIKNVLKTIYDKSYEQEHWKNNNLYATSLEFRLLRQHGFNL 120

Query: 166 QQDVFNHFFSNETKSFNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMK 225
            QDVFN+F+S+ETKSFN Q+Y+DLNGML LYEASFLSIEGENILETAKHFT+EYLEKYMK
Sbjct: 121 SQDVFNNFYSSETKSFNTQMYQDLNGMLFLYEASFLSIEGENILETAKHFTVEYLEKYMK 180

Query: 226 SSTDENEVAIVRHALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIH 285
           SS DENEVAIVRHALELPLHWRMPRL+TRWFIDIYERKVDMNPILLEFAKLDFN+VQ IH
Sbjct: 181 SSKDENEVAIVRHALELPLHWRMPRLDTRWFIDIYERKVDMNPILLEFAKLDFNRVQSIH 240

Query: 286 QQDLKYASR 295
           QQDLKY SR
Sbjct: 241 QQDLKYTSR 248

BLAST of Csa2G299920 vs. TrEMBL
Match: F6I5S6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g03700 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 7.5e-111
Identity = 200/327 (61.16%), Postives = 258/327 (78.90%), Query Frame = 1

Query: 49  QTVVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLE 108
           +T VRR+ NYQ   W Y+++QSL S++ GE  +G F++LK EV++++ K+  + PL QLE
Sbjct: 24  ETTVRRTANYQAPIWDYDYVQSLRSDYTGETCIGRFDQLKREVKMMLGKV--EKPLDQLE 83

Query: 109 FIDILQRLGISYHFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQD 168
            ID+LQRLGISY FE+EIK +L   Y NH  +D WK  NL+AT+LEFR+LRQ G++I QD
Sbjct: 84  LIDLLQRLGISYQFEDEIKRLLNSIYCNHNIDDKWKKENLHATALEFRILRQNGYSIPQD 143

Query: 169 VFNHFFSNETKSFNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSST 228
           VF+ F  +E   F   L ED+ G+LCLYEAS+LSIEGE+ILE A+ FT ++LE  ++ + 
Sbjct: 144 VFSSF-KDEMGGFKACLSEDIQGILCLYEASYLSIEGESILEEARDFTKKHLEGCLRQNI 203

Query: 229 DENEVAIVRHALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQD 288
           DEN   +V HALELPLHWRM RLE RWFID YER+ DMNPILLEFAKLD+N VQ  HQ+D
Sbjct: 204 DENLAILVSHALELPLHWRMLRLEARWFIDAYERRQDMNPILLEFAKLDYNMVQAKHQED 263

Query: 289 LKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDV 348
           LKYASRWW+S+ LG+KLSFARDRL+E+F WTVG+ FEP+F Y RR LTK+NALIT IDDV
Sbjct: 264 LKYASRWWRSTRLGEKLSFARDRLMENFLWTVGVIFEPQFGYCRRMLTKVNALITTIDDV 323

Query: 349 YDVYGSLDELELFTNAVER-DLDELER 375
           YDVYG+L+ELELFT+AV+R D++ +++
Sbjct: 324 YDVYGTLEELELFTDAVDRWDINAMDQ 347

BLAST of Csa2G299920 vs. TrEMBL
Match: F6I5S6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g03700 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.6e-28
Identity = 67/99 (67.68%), Postives = 81/99 (81.82%), Query Frame = 1

Query: 370 DELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVER 429
           DEL+RGDVPKSIQCYM++TGASE +ARE+I+ LI ETWKK+N+  V    S F + F+  
Sbjct: 469 DELKRGDVPKSIQCYMHETGASEEDAREHIKCLIGETWKKMNEDRVME--SPFSQTFIGI 528

Query: 430 AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISI 469
           A NLARMAQCMYQYGDGHG+  +ETKDRV+SLLIEPI +
Sbjct: 529 AINLARMAQCMYQYGDGHGVQDRETKDRVLSLLIEPIPL 565


HSP 2 Score: 397.1 bits (1019), Expect = 3.0e-107
Identity = 194/332 (58.43%), Postives = 254/332 (76.51%), Query Frame = 1

Query: 42  GGARMCSQT--VVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIA 101
           GGA+  SQT  +VRR+ +YQPS W +++IQSL+S++ GE Y     +LKG+V++++ ++ 
Sbjct: 41  GGAKTSSQTAVIVRRTASYQPSIWDHDYIQSLTSDYVGETYTRQLEKLKGDVKIMLGQVG 100

Query: 102 NDDPLKQLEFIDILQRLGISYHFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLR 161
             +PL QLE ID LQRLGI YHF  EIK +L   Y N+  ND WK  +LYAT+LEFRLLR
Sbjct: 101 --EPLHQLELIDTLQRLGIHYHFGEEIKRILHSIYNNYNRNDTWKNGDLYATALEFRLLR 160

Query: 162 QYGFNIQQDVFNHFFSNETKSFNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEY 221
           Q+G+++ QDVF H F N+  +    L ED+ G+LCLYEAS+LS+EGENILE A+ FT  +
Sbjct: 161 QHGYHVPQDVF-HIFINKMGTVKPWLNEDIKGILCLYEASYLSVEGENILEEARDFTRNF 220

Query: 222 LEKYMKSSTDENEV-AIVRHALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDF 281
           LE+Y++ + D+N++ AI+ HA+ELPLHWRM RLE RWFID+YER   MNPILLE AKLD+
Sbjct: 221 LEEYLERTVDQNDLTAIINHAMELPLHWRMLRLEARWFIDVYERSQGMNPILLELAKLDY 280

Query: 282 NKVQFIHQQDLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKI 341
           N VQ  +Q+DLK+AS WW+S+ L +K SFARDRL+E+F W VG  FEP+F Y RR LTK+
Sbjct: 281 NMVQATYQEDLKHASMWWRSTRLPEKSSFARDRLVENFLWAVGFIFEPQFGYCRRMLTKL 340

Query: 342 NALITIIDDVYDVYGSLDELELFTNAVERDLD 371
            +LIT IDDVYDVYG+LDELELFT+AV+R  D
Sbjct: 341 ISLITTIDDVYDVYGTLDELELFTDAVDRGWD 369

BLAST of Csa2G299920 vs. TAIR10
Match: AT3G25830.1 (AT3G25830.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 297.0 bits (759), Expect = 2.1e-80
Identity = 157/342 (45.91%), Postives = 232/342 (67.84%), Query Frame = 1

Query: 53  RRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDI 112
           RRSGNYQPS W + ++ S+ +++  E  V   + LK +V+ +++       L+QLE ID 
Sbjct: 47  RRSGNYQPSPWNHCYLLSIENKYASETEVITRDVLKKKVKSMLDD-EKKSRLEQLELIDD 106

Query: 113 LQRLGISYHFENEIKDMLTMTYKNHCENDDWKIN---NLYATSLEFRLLRQYGFNIQQDV 172
           LQ+LG+SYHFE EI D LT  +     N  WK +   +L+ATSLEFRLLRQ+GF++ +++
Sbjct: 107 LQKLGVSYHFEIEINDTLTDLHLKMGRNC-WKCDKEEDLHATSLEFRLLRQHGFDVSENI 166

Query: 173 FNHFFSN-ETKSFNVQLYEDLNGMLCLYEASFLSIEGENIL-ETAKHFTMEYLEKYMKSS 232
           F+      E+ +F      ++NG++ LYEAS+LS + +  L +  + F  E + K++   
Sbjct: 167 FDVIIDQIESNTFKTN---NINGIISLYEASYLSTKSDTKLHKVIRPFATEQIRKFVDDE 226

Query: 233 TDENEVAIVR--HALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIH 292
             +N     +  HALE+P HWRM RL+TRW+ID YE+K DMN +L+EFAK+DFN VQ  H
Sbjct: 227 DTKNIEVREKAYHALEMPYHWRMRRLDTRWYIDAYEKKHDMNLVLIEFAKIDFNIVQAAH 286

Query: 293 QQDLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITII 352
           Q+DLKY SRWWK + L  +L F RDR++E++FWTVG+ +EP+F Y RR +T +NAL+T I
Sbjct: 287 QEDLKYVSRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIRRIMTIVNALVTTI 346

Query: 353 DDVYDVYGSLDELELFTNAVER-DLDELERGDVPKSIQ-CYM 386
           DD+YD+YG+L+ELELFT+ VE  D++ L  G++P+ ++ C++
Sbjct: 347 DDIYDIYGTLEELELFTSMVENWDVNRL--GELPEYMRLCFL 381


HSP 2 Score: 105.9 bits (263), Expect = 6.9e-23
Identity = 54/100 (54.00%), Postives = 69/100 (69.00%), Query Frame = 1

Query: 371 ELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLN-KTEVENNYSIFPRVFVER 430
           EL RGDV KS+QCYM++TGASE  AR+++  +I + W  +N +T+   N S   R F E 
Sbjct: 495 ELARGDVLKSVQCYMHETGASEERARDHVHQMISDMWDDMNSETKTACNSSSRSRGFKEA 554

Query: 431 AKNLARMAQCMYQYGDGHGMGYQ-ETKDRVMSLLIEPISI 469
           A NLARM+QCMYQYGDGHG   + +T DRV SLL++PI +
Sbjct: 555 AMNLARMSQCMYQYGDGHGCPEKAKTIDRVQSLLVDPIPL 594

BLAST of Csa2G299920 vs. TAIR10
Match: AT3G25820.1 (AT3G25820.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 297.0 bits (759), Expect = 2.1e-80
Identity = 157/342 (45.91%), Postives = 232/342 (67.84%), Query Frame = 1

Query: 53  RRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDI 112
           RRSGNYQPS W + ++ S+ +++  E  V   + LK +V+ +++       L+QLE ID 
Sbjct: 47  RRSGNYQPSPWNHCYLLSIENKYASETEVITRDVLKKKVKSMLDD-EKKSRLEQLELIDD 106

Query: 113 LQRLGISYHFENEIKDMLTMTYKNHCENDDWKIN---NLYATSLEFRLLRQYGFNIQQDV 172
           LQ+LG+SYHFE EI D LT  +     N  WK +   +L+ATSLEFRLLRQ+GF++ +++
Sbjct: 107 LQKLGVSYHFEIEINDTLTDLHLKMGRNC-WKCDKEEDLHATSLEFRLLRQHGFDVSENI 166

Query: 173 FNHFFSN-ETKSFNVQLYEDLNGMLCLYEASFLSIEGENIL-ETAKHFTMEYLEKYMKSS 232
           F+      E+ +F      ++NG++ LYEAS+LS + +  L +  + F  E + K++   
Sbjct: 167 FDVIIDQIESNTFKTN---NINGIISLYEASYLSTKSDTKLHKVIRPFATEQIRKFVDDE 226

Query: 233 TDENEVAIVR--HALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIH 292
             +N     +  HALE+P HWRM RL+TRW+ID YE+K DMN +L+EFAK+DFN VQ  H
Sbjct: 227 DTKNIEVREKAYHALEMPYHWRMRRLDTRWYIDAYEKKHDMNLVLIEFAKIDFNIVQAAH 286

Query: 293 QQDLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITII 352
           Q+DLKY SRWWK + L  +L F RDR++E++FWTVG+ +EP+F Y RR +T +NAL+T I
Sbjct: 287 QEDLKYVSRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIRRIMTIVNALVTTI 346

Query: 353 DDVYDVYGSLDELELFTNAVER-DLDELERGDVPKSIQ-CYM 386
           DD+YD+YG+L+ELELFT+ VE  D++ L  G++P+ ++ C++
Sbjct: 347 DDIYDIYGTLEELELFTSMVENWDVNRL--GELPEYMRLCFL 381


HSP 2 Score: 105.9 bits (263), Expect = 6.9e-23
Identity = 54/100 (54.00%), Postives = 69/100 (69.00%), Query Frame = 1

Query: 371 ELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLN-KTEVENNYSIFPRVFVER 430
           EL RGDV KS+QCYM++TGASE  AR+++  +I + W  +N +T+   N S   R F E 
Sbjct: 495 ELARGDVLKSVQCYMHETGASEERARDHVHQMISDMWDDMNSETKTACNSSSRSRGFKEA 554

Query: 431 AKNLARMAQCMYQYGDGHGMGYQ-ETKDRVMSLLIEPISI 469
           A NLARM+QCMYQYGDGHG   + +T DRV SLL++PI +
Sbjct: 555 AMNLARMSQCMYQYGDGHGCPEKAKTIDRVQSLLVDPIPL 594

BLAST of Csa2G299920 vs. TAIR10
Match: AT3G25810.1 (AT3G25810.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 290.0 bits (741), Expect = 2.6e-78
Identity = 146/330 (44.24%), Postives = 222/330 (67.27%), Query Frame = 1

Query: 53  RRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDI 112
           RRSGNYQPS+W + ++ S+ +++  E  V   + LK +V+ ++ ++     L++LE ID 
Sbjct: 47  RRSGNYQPSSWDHSYLLSIENKYVNEKEVITRHVLKKKVKKMLEEVETKSRLEKLELIDD 106

Query: 113 LQRLGISYHFENEIKDMLTMTYKNHCENDDWKIN---NLYATSLEFRLLRQYGFNIQQDV 172
           LQ+LG+SYHFE EI ++LT  +  + +N  WK +   +L+AT+LEFRLLRQ+GF + +D+
Sbjct: 107 LQKLGVSYHFEQEINNILTNFHLENGKNI-WKCDKEEDLHATALEFRLLRQHGFGVSEDI 166

Query: 173 FNHFFSN-ETKSFNVQLYEDLNGMLCLYEASFLSIEGENIL-ETAKHFTMEYLEKYMKSS 232
           F+      E+ +F     +++  ++ LYEAS+LS + +  L +  + F  E +  ++   
Sbjct: 167 FDVIIDKIESNTFKS---DNITSIITLYEASYLSTKSDTKLHKVIRPFATEQIRNFVDDE 226

Query: 233 TDENEVAIVR---HALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFI 292
           ++   + +     HALE+P HWRM RLETRW+ID YE+K DMN  L EFAK+DFN VQ  
Sbjct: 227 SETYNIMLREMAIHALEIPYHWRMRRLETRWYIDAYEKKHDMNLFLAEFAKIDFNIVQTA 286

Query: 293 HQQDLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITI 352
           HQ+D+KY S WWK +GLG +L F RDR++E++FWTVG+ +EP+F Y RR +  + ALIT+
Sbjct: 287 HQEDVKYVSCWWKETGLGSQLHFVRDRIVENYFWTVGMIYEPQFGYIRRIVAIVAALITV 346

Query: 353 IDDVYDVYGSLDELELFTNAVER-DLDELE 374
           IDD+YD+YG+ +ELELFT  V+  D++ L+
Sbjct: 347 IDDIYDIYGTPEELELFTAMVQNWDINRLD 372


HSP 2 Score: 104.0 bits (258), Expect = 2.6e-22
Identity = 55/134 (41.04%), Postives = 77/134 (57.46%), Query Frame = 1

Query: 338 INALITIIDDVYDVYGSLDELELFTNAVERDLDELERGDVPKSIQCYMNDTGASESNARE 397
           +  L +++    DV      +    N +    DEL RGDV KS+QCYM++TG SE  AR 
Sbjct: 464 VQILESLVQQQQDVVRCSATVLRLANDLATSPDELARGDVLKSVQCYMHETGVSEEEART 523

Query: 398 YIRHLIDETWKKLN--KTEVENNYSIFPRVFVERAKNLARMAQCMYQYGDGHGM-GYQET 457
           +++ +I  TW ++N        + S+  R FVE A NLARM+QCMYQ+GDGHG     + 
Sbjct: 524 HVQQMISHTWDEMNYEARTAARSSSLLSRRFVETAMNLARMSQCMYQHGDGHGCPDKAKI 583

Query: 458 KDRVMSLLIEPISI 469
            DRV +LL++PI +
Sbjct: 584 VDRVQTLLVDPIPL 597

BLAST of Csa2G299920 vs. TAIR10
Match: AT4G16740.1 (AT4G16740.1 terpene synthase 03)

HSP 1 Score: 289.7 bits (740), Expect = 3.4e-78
Identity = 158/335 (47.16%), Postives = 219/335 (65.37%), Query Frame = 1

Query: 51  VVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFI 110
           V RRS NYQPS W++E++ SL + +  E  V     LK EV  ++N+   +  L+QLE I
Sbjct: 24  VSRRSANYQPSLWQHEYLLSLGNTYVKEDNVERVTLLKQEVSKMLNE--TEGLLEQLELI 83

Query: 111 DILQRLGISYHFENEIKDMLT-------MTYKNHCENDDWKINNLYATSLEFRLLRQYGF 170
           D LQRLG+SYHFE EIK  LT         +KN  + + W   +LYAT+LEFRLLRQ+GF
Sbjct: 84  DTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRW--GDLYATALEFRLLRQHGF 143

Query: 171 NIQQDVFNHFFSNETKSFNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKY 230
           +I QDVF+     +         +D+ G+L LYEAS+LS   +  L+ + ++T + L K+
Sbjct: 144 SIAQDVFDGNIGVDLDD------KDIKGILSLYEASYLSTRIDTKLKESIYYTTKRLRKF 203

Query: 231 MKSSTDENEVAIVR----HALELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFN 290
           ++ + +E +   +R    HALE+P H R+ RLE RW+I++Y  + DMNPILLE AKLDFN
Sbjct: 204 VEVNKNETKSYTLRRMVIHALEMPYHRRVGRLEARWYIEVYGERHDMNPILLELAKLDFN 263

Query: 291 KVQFIHQQDLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKIN 350
            VQ IHQ +LK  S WW  +GL + L F RDR+ E +F +VG+ +EPEF+Y R+ LTK+ 
Sbjct: 264 FVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEGYFSSVGVMYEPEFAYHRQMLTKVF 323

Query: 351 ALITIIDDVYDVYGSLDELELFTNAVER-DLDELE 374
            LIT IDD+YD+YG+L+EL+LFT  VE+ D++ LE
Sbjct: 324 MLITTIDDIYDIYGTLEELQLFTTIVEKWDVNRLE 348


HSP 2 Score: 93.6 bits (231), Expect = 3.5e-19
Identity = 58/164 (35.37%), Postives = 91/164 (55.49%), Query Frame = 1

Query: 323 GFEPEFSYFRRSLTKINALITII--------DDVYDVYGSLDELELFTNA----VERDL- 382
           G++P F  + ++    +++ TI+        D   D+ GS +   + ++A    +  DL 
Sbjct: 405 GYKPNFEEYMQNGWISSSVPTILLHLFCLLSDQTLDILGSYNHSVVRSSATILRLANDLA 464

Query: 383 ---DELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVF 442
              +EL RGD  KS+QC+M++TGASE+ +R YI+ +I   W  LN   +E       + F
Sbjct: 465 TSSEELARGDTMKSVQCHMHETGASEAESRAYIQGIIGVAWDDLN---MEKKSCRLHQGF 524

Query: 443 VERAKNLARMAQCMYQYGDGHGM-GYQETKDRVMSLLIEPISIH 470
           +E A NL R+AQC+YQYGDGHG     +T + V SLL+ P+ ++
Sbjct: 525 LEAAANLGRVAQCVYQYGDGHGCPDKAKTVNHVRSLLVHPLPLN 565

BLAST of Csa2G299920 vs. TAIR10
Match: AT2G24210.1 (AT2G24210.1 terpene synthase 10)

HSP 1 Score: 281.2 bits (718), Expect = 1.2e-75
Identity = 158/365 (43.29%), Postives = 243/365 (66.58%), Query Frame = 1

Query: 48  SQTVVRRSGNYQPSTWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDP---L 107
           S TV RRS NYQPS W +  + S+ ++F  +  V   + LK +VR    K+ ND+    L
Sbjct: 41  SPTVQRRSANYQPSRWDHHHLLSVENKFAKDKRVRERDLLKEKVR----KMLNDEQKTYL 100

Query: 108 KQLEFIDILQRLGISYHFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFN 167
            QLEFID LQ+LG+SYHFE EI ++LT +YK   +  + + ++L+AT+LEFRL RQ+GFN
Sbjct: 101 DQLEFIDDLQKLGVSYHFEAEIDNILTSSYKK--DRTNIQESDLHATALEFRLFRQHGFN 160

Query: 168 IQQDVFNHFFSNETKSFNVQLYEDLNGMLCLYEASFLSIE-GENILETAKHFTMEYLEKY 227
           + +DVF+ F  N  K       +D+ G++ LYEAS+LS +  +N+    + F  + L  +
Sbjct: 161 VSEDVFDVFMENCGKFDR----DDIYGLISLYEASYLSTKLDKNLQIFIRPFATQQLRDF 220

Query: 228 MKSSTDEN-----EVAIVRHALELPLHWRMPRLETRWFIDIYERKVDM-NPILLEFAKLD 287
           + + ++E+      V IV  AL++P +W+M RL TRW+ID+Y ++ +  N +++EFAK+D
Sbjct: 221 VDTHSNEDFGSCDMVEIVVQALDMPYYWQMRRLSTRWYIDVYGKRQNYKNLVVVEFAKID 280

Query: 288 FNKVQFIHQQDLKYASRWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTK 347
           FN VQ IHQ++LK  S WW  +GLG++L FARDR++E++FWT+G   EP++ Y R+++TK
Sbjct: 281 FNIVQAIHQEELKNVSSWWMETGLGKQLYFARDRIVENYFWTIGQIQEPQYGYVRQTMTK 340

Query: 348 INALITIIDDVYDVYGSLDELELFTNAVER-DLDELERGDVPKSIQ-CYMNDTGASESNA 401
           INAL+T IDD+YD+YG+L+EL+LFT A E  D++ L+  ++P+ ++ C++       S A
Sbjct: 341 INALLTTIDDIYDIYGTLEELQLFTVAFENWDINRLD--ELPEYMRLCFLVIYNEVNSIA 393


HSP 2 Score: 113.6 bits (283), Expect = 3.3e-25
Identity = 81/236 (34.32%), Postives = 120/236 (50.85%), Query Frame = 1

Query: 241 ELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKL-DFNKVQFIHQQ--DLKYA----S 300
           ELP + R+       F+ IY    ++N I  E  +  + N + F+ +   D+  A    +
Sbjct: 368 ELPEYMRL------CFLVIYN---EVNSIACEILRTKNINVIPFLKKSWTDVSKAYLVEA 427

Query: 301 RWWKSSGLGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYG 360
           +W+KS        + ++  I     T+ + F   FS        I  L T+     +V  
Sbjct: 428 KWYKSGHKPNLEEYMQNARISISSPTIFVHFYCVFS----DQLSIQVLETLSQHQQNVVR 487

Query: 361 SLDELELFTNAVERDLDELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKT 420
               +    N +    DEL RGDV KSIQCYM++TGASE  AR ++R +I++ W ++N  
Sbjct: 488 CSSSVFRLANDLVTSPDELARGDVCKSIQCYMSETGASEDKARSHVRQMINDLWDEMNYE 547

Query: 421 EVENNYSIFPRVFVERAKNLARMAQCMYQYGDGHGMGYQ-ETKDRVMSLLIEPISI 469
           ++ ++ SI    F+E   NLARM+QCMYQYGDGHG   + +  DRVMSLL  PI +
Sbjct: 548 KMAHSSSILHHDFMETVINLARMSQCMYQYGDGHGSPEKAKIVDRVMSLLFNPIPL 590

BLAST of Csa2G299920 vs. NCBI nr
Match: gi|700207003|gb|KGN62122.1| (hypothetical protein Csa_2G299920 [Cucumis sativus])

HSP 1 Score: 967.6 bits (2500), Expect = 7.9e-279
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 1

Query: 1   MAHLHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQP 60
           MAHLHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQP
Sbjct: 1   MAHLHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQP 60

Query: 61  STWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISY 120
           STWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISY
Sbjct: 61  STWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISY 120

Query: 121 HFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKS 180
           HFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKS
Sbjct: 121 HFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKS 180

Query: 181 FNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHAL 240
           FNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHAL
Sbjct: 181 FNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHAL 240

Query: 241 ELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSG 300
           ELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSG
Sbjct: 241 ELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSG 300

Query: 301 LGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELEL 360
           LGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELEL
Sbjct: 301 LGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELEL 360

Query: 361 FTNAVERDLDELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYS 420
           FTNAVERDLDELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYS
Sbjct: 361 FTNAVERDLDELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYS 420

Query: 421 IFPRVFVERAKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE 475
           IFPRVFVERAKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE
Sbjct: 421 IFPRVFVERAKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE 474

BLAST of Csa2G299920 vs. NCBI nr
Match: gi|449465932|ref|XP_004150681.1| (PREDICTED: terpene synthase 10-like [Cucumis sativus])

HSP 1 Score: 747.7 bits (1929), Expect = 1.3e-212
Identity = 367/367 (100.00%), Postives = 367/367 (100.00%), Query Frame = 1

Query: 1   MAHLHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQP 60
           MAHLHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQP
Sbjct: 1   MAHLHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQP 60

Query: 61  STWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISY 120
           STWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISY
Sbjct: 61  STWKYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISY 120

Query: 121 HFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKS 180
           HFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKS
Sbjct: 121 HFENEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKS 180

Query: 181 FNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHAL 240
           FNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHAL
Sbjct: 181 FNVQLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHAL 240

Query: 241 ELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSG 300
           ELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSG
Sbjct: 241 ELPLHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSG 300

Query: 301 LGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELEL 360
           LGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELEL
Sbjct: 301 LGQKLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELEL 360

Query: 361 FTNAVER 368
           FTNAVER
Sbjct: 361 FTNAVER 367

BLAST of Csa2G299920 vs. NCBI nr
Match: gi|449465932|ref|XP_004150681.1| (PREDICTED: terpene synthase 10-like [Cucumis sativus])

HSP 1 Score: 220.7 bits (561), Expect = 5.4e-54
Identity = 105/105 (100.00%), Postives = 105/105 (100.00%), Query Frame = 1

Query: 370 DELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVER 429
           DELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVER
Sbjct: 496 DELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVER 555

Query: 430 AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE 475
           AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE
Sbjct: 556 AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE 600


HSP 2 Score: 648.7 bits (1672), Expect = 8.1e-183
Identity = 321/364 (88.19%), Postives = 337/364 (92.58%), Query Frame = 1

Query: 4   LHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQPSTW 63
           LHQI+ + FP SS+V ERV SFNHNSLFVPRV+NNVT G ARMCSQT+VRRSGNYQP TW
Sbjct: 3   LHQIYFTSFPASSVV-ERVYSFNHNSLFVPRVMNNVTSG-ARMCSQTIVRRSGNYQPPTW 62

Query: 64  KYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISYHFE 123
           K+EFIQSLSSEF GEIYVG FNELKGEVRV+MNKIANDDPLKQLEFIDILQRLGISYHFE
Sbjct: 63  KHEFIQSLSSEFAGEIYVGRFNELKGEVRVVMNKIANDDPLKQLEFIDILQRLGISYHFE 122

Query: 124 NEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKSFNV 183
           NEIKDMLT TYK  CENDDWK NNLYATSLEFRLLRQ+GFNI QD+FN FF NETKSFNV
Sbjct: 123 NEIKDMLTTTYKKCCENDDWKCNNLYATSLEFRLLRQHGFNIPQDIFNPFFFNETKSFNV 182

Query: 184 QLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHALELP 243
           QLYEDL GML LYEASFLSIEGENILETAKHFT EYLEKYMKSS DENEVAIVRHALELP
Sbjct: 183 QLYEDLKGMLYLYEASFLSIEGENILETAKHFTREYLEKYMKSSKDENEVAIVRHALELP 242

Query: 244 LHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSGLGQ 303
           LHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQ IHQQDLKYASRWW+ SGLG+
Sbjct: 243 LHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQSIHQQDLKYASRWWRRSGLGE 302

Query: 304 KLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELELFTN 363
           KLSFARDRL+E+FFWTVG+GFEPE SYFRR  TK+NALIT IDDVYDVYGSLDELELFT+
Sbjct: 303 KLSFARDRLMENFFWTVGVGFEPELSYFRRMTTKVNALITTIDDVYDVYGSLDELELFTS 362

Query: 364 AVER 368
           AVER
Sbjct: 363 AVER 364

BLAST of Csa2G299920 vs. NCBI nr
Match: gi|659122004|ref|XP_008460926.1| (PREDICTED: terpene synthase 10-like [Cucumis melo])

HSP 1 Score: 206.1 bits (523), Expect = 1.4e-49
Identity = 97/105 (92.38%), Postives = 101/105 (96.19%), Query Frame = 1

Query: 370 DELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVER 429
           DELERGDVPKSIQCYMNDTGASE+ AREYI HLIDETWKKLNKTE ENNYSI+PRVF+ER
Sbjct: 493 DELERGDVPKSIQCYMNDTGASENIAREYIGHLIDETWKKLNKTEFENNYSIYPRVFIER 552

Query: 430 AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE 475
           AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHC +SE
Sbjct: 553 AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCHHSE 597


HSP 2 Score: 555.8 bits (1431), Expect = 7.1e-155
Identity = 274/364 (75.27%), Postives = 309/364 (84.89%), Query Frame = 1

Query: 4   LHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQPSTW 63
           LHQ+F +F  +S  +     +F+H  + VPR    +  G A MC++T+VRRSGNY+P  W
Sbjct: 3   LHQLFATF-SISPFIQRISFNFDHLCVHVPRTFKVI--GAATMCNKTIVRRSGNYRPPIW 62

Query: 64  KYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISYHFE 123
           K+EFIQSL SEFE EIY+G FNELKGE+RVIMN I  DDPLKQLE ID+LQRLGISYHFE
Sbjct: 63  KHEFIQSLRSEFEEEIYIGRFNELKGEIRVIMNTII-DDPLKQLELIDMLQRLGISYHFE 122

Query: 124 NEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKSFNV 183
           NEIK++L  TY    E + WK NNLYATSLEFRLLRQ+GFN+ QDVFNHFFS+ETKSFNV
Sbjct: 123 NEIKNVLKTTYDTSYEKEHWKNNNLYATSLEFRLLRQHGFNLSQDVFNHFFSDETKSFNV 182

Query: 184 QLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHALELP 243
           QLYEDLNG+L LYEASFLS E E ILETAKHFTMEYLEKYMKSS DENEVAIVRHALELP
Sbjct: 183 QLYEDLNGVLYLYEASFLSTEDEYILETAKHFTMEYLEKYMKSSKDENEVAIVRHALELP 242

Query: 244 LHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSGLGQ 303
           LHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFN+VQ IHQQDLKY S WWKSSGLG+
Sbjct: 243 LHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNRVQSIHQQDLKYTSSWWKSSGLGE 302

Query: 304 KLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELELFTN 363
           KLSFAR+RL+E+F W+VG G+EPEFSY+RR  TKIN  IT IDDVYDVYG+LDEL+LFT+
Sbjct: 303 KLSFARNRLMENFLWSVGFGYEPEFSYYRRMATKINVFITTIDDVYDVYGTLDELQLFTD 362

Query: 364 AVER 368
           A+ER
Sbjct: 363 AIER 362

BLAST of Csa2G299920 vs. NCBI nr
Match: gi|659122225|ref|XP_008461030.1| (PREDICTED: terpene synthase 10-like [Cucumis melo])

HSP 1 Score: 179.1 bits (453), Expect = 1.8e-41
Identity = 87/105 (82.86%), Postives = 96/105 (91.43%), Query Frame = 1

Query: 370 DELERGDVPKSIQCYMNDTGASESNAREYIRHLIDETWKKLNKTEVENNYSIFPRVFVER 429
           DEL+RGDVPKSIQCYMNDTGASESNAR+YI+HLIDETWKK+NK EVEN   I PRVFV+R
Sbjct: 491 DELKRGDVPKSIQCYMNDTGASESNARKYIKHLIDETWKKMNKIEVEN--PIIPRVFVDR 550

Query: 430 AKNLARMAQCMYQYGDGHGMGYQETKDRVMSLLIEPISIHCSYSE 475
           AKNLARMAQCMYQYGDGHG  ++ETKDRVMSLLI+PIS+H SY E
Sbjct: 551 AKNLARMAQCMYQYGDGHGTAHEETKDRVMSLLIQPISVH-SYGE 592


HSP 2 Score: 547.7 bits (1410), Expect = 1.9e-152
Identity = 272/372 (73.12%), Postives = 317/372 (85.22%), Query Frame = 1

Query: 4   LHQIFTSFFPVSSLVMERVSSFNHNSLFVPRVINNVTGGGARMCSQTVVRRSGNYQPSTW 63
           LHQ  TS    +S   ERVS  N N  F+PR++  ++     MCS+T+VRRSGNYQP  W
Sbjct: 3   LHQFPTS--SQTSRFFERVSC-NFNPSFMPRIVKAISVTTRIMCSRTIVRRSGNYQPPIW 62

Query: 64  KYEFIQSLSSEFEGEIYVGLFNELKGEVRVIMNKIANDDPLKQLEFIDILQRLGISYHFE 123
           K+EFIQSL SEF  EIYVG FNELK E+R+I+N+I  DDPL+QLE +D LQRLGISYHFE
Sbjct: 63  KHEFIQSLRSEFAEEIYVGRFNELKREIRLIVNQII-DDPLRQLELVDTLQRLGISYHFE 122

Query: 124 NEIKDMLTMTYKNHCENDDWKINNLYATSLEFRLLRQYGFNIQQDVFNHFFSNETKSFNV 183
           NEIK++L   ++   END WK  NLYATSLEFRLLRQ+GFN+ QDVFN+F+S+ETKSF+ 
Sbjct: 123 NEIKNVLKTAFEKSYENDYWKKKNLYATSLEFRLLRQHGFNLSQDVFNNFYSDETKSFST 182

Query: 184 QLYEDLNGMLCLYEASFLSIEGENILETAKHFTMEYLEKYMKSSTDENEVAIVRHALELP 243
           QLYE+LNG+LCL+EASFLSIEGENILETAKHFTMEYLEKY+KSS DENEVAIVRHALELP
Sbjct: 183 QLYEELNGILCLHEASFLSIEGENILETAKHFTMEYLEKYIKSSKDENEVAIVRHALELP 242

Query: 244 LHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQFIHQQDLKYASRWWKSSGLGQ 303
           LHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQ IHQQDLKYAS WW+S+G G+
Sbjct: 243 LHWRMPRLETRWFIDIYERKVDMNPILLEFAKLDFNKVQSIHQQDLKYASSWWRSTGFGE 302

Query: 304 KLSFARDRLIESFFWTVGIGFEPEFSYFRRSLTKINALITIIDDVYDVYGSLDELELFTN 363
           KLSFARDR++E+F WTVG G+EPEFS++RR  TKINA IT IDDVYDVYG+LDEL+LFT+
Sbjct: 303 KLSFARDRVMENFLWTVGFGYEPEFSFYRRMATKINAFITTIDDVYDVYGTLDELQLFTD 362

Query: 364 AVER-DLDELER 375
           A+ER D+D L++
Sbjct: 363 AIERWDVDALDQ 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ATESY_VITVI4.2e-10750.53(-)-alpha-terpineol synthase OS=Vitis vinifera PE=1 SV=1[more]
MYRS_QUEIL2.7e-10658.23Myrcene synthase, chloroplastic OS=Quercus ilex PE=1 SV=1[more]
TPS10_RICCO1.2e-10455.59Terpene synthase 10 OS=Ricinus communis GN=TPS10 PE=2 SV=1[more]
TPS2_CANSA1.5e-9149.35(+)-alpha-pinene synthase, chloroplastic OS=Cannabis sativa PE=1 SV=1[more]
RLC1_CITLI7.3e-9151.99(R)-limonene synthase 1 OS=Citrus limon PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LMX2_CUCSA5.5e-279100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299920 PE=3 SV=1[more]
A0A0A0LM62_CUCSA6.8e-12077.05Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299890 PE=3 SV=1[more]
A0A0A0LM58_CUCSA1.8e-11281.53Uncharacterized protein OS=Cucumis sativus GN=Csa_2G299350 PE=3 SV=1[more]
F6I5S6_VITVI7.5e-11161.16Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g03700 PE=3 SV=... [more]
F6I5S6_VITVI3.6e-2867.68Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g03700 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G25830.12.1e-8045.91 terpene synthase-like sequence-1,8-cineole[more]
AT3G25820.12.1e-8045.91 terpene synthase-like sequence-1,8-cineole[more]
AT3G25810.12.6e-7844.24 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
AT4G16740.13.4e-7847.16 terpene synthase 03[more]
AT2G24210.11.2e-7543.29 terpene synthase 10[more]
Match NameE-valueIdentityDescription
gi|700207003|gb|KGN62122.1|7.9e-279100.00hypothetical protein Csa_2G299920 [Cucumis sativus][more]
gi|449465932|ref|XP_004150681.1|1.3e-212100.00PREDICTED: terpene synthase 10-like [Cucumis sativus][more]
gi|449465932|ref|XP_004150681.1|5.4e-54100.00PREDICTED: terpene synthase 10-like [Cucumis sativus][more]
gi|659122004|ref|XP_008460926.1|1.4e-4992.38PREDICTED: terpene synthase 10-like [Cucumis melo][more]
gi|659122225|ref|XP_008461030.1|1.8e-4182.86PREDICTED: terpene synthase 10-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001906Terpene_synth_N
IPR005630Terpene_synthase_metal-bd
IPR008930Terpenoid_cyclase/PrenylTrfase
IPR008949Isoprenoid_synthase_dom_sf
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0010333terpene synthase activity
GO:0016829lyase activity
GO:0000287magnesium ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0051762 sesquiterpene biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0010334 sesquiterpene synthase activity
molecular_function GO:0016740 transferase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU126443cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa2G299920.1Csa2G299920.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU126443CU126443transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001906Terpene synthase, N-terminal domainGENE3DG3DSA:1.50.30.10coord: 102..249
score: 1.2
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 63..240
score: 1.3
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 370..409
score: 3.4E-7coord: 271..367
score: 8.8
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidunknownSSF48239Terpenoid cyclases/Protein prenyltransferasescoord: 59..263
score: 6.83
IPR008949Isoprenoid synthase domainGENE3DG3DSA:1.10.600.10coord: 370..466
score: 2.2E-28coord: 250..367
score: 2.0
IPR008949Isoprenoid synthase domainunknownSSF48576Terpenoid synthasescoord: 267..367
score: 6.33E-37coord: 371..466
score: 8.63
NoneNo IPR availablePANTHERPTHR31225FAMILY NOT NAMEDcoord: 47..468
score: 4.7E
NoneNo IPR availablePANTHERPTHR31225:SF161,8-CINEOLE SYNTHASE 1, CHLOROPLASTIC-RELATEDcoord: 47..468
score: 4.7E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Csa2G299920Lsi10G006910Bottle gourd (USVL1VR-Ls)culsiB093
Csa2G299920CmoCh20G011270Cucurbita moschata (Rifu)cmocuB530
The following gene(s) are paralogous to this gene:

None