Csa2G022820 (gene) Cucumber (Chinese Long) v2

NameCsa2G022820
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionPentatricopeptide repeat-containing protein; contains IPR002885 (Pentatricopeptide repeat), IPR011990 (Tetratricopeptide-like helical)
LocationChr2 : 2739337 .. 2741597 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCATTTTTGAATAAATGAACTTGAGAGGGAGAGATTAAGAATGTGAGAGAGAGACCAAAAACTGGTCACCGTCTCCGATTCCCAATTTCATGGATGAAACTCAAATGGGTATTTCAAAAACGGAGCTCACACCTTCCCTCTTGGGTCACTTCTCTAATCTCCCCTTTCAGAAACCAATTCCATCAAAACCCATTTCCAGAAACCTCCTCAACATTCGTCCTCAACCATCTAGACCCAAGCTTCCTTCTATCCATTTGTGGAAGAGAAGGGAACCTCCATTTGGGTTCTTCTCTCCATGCATCCATCATCAAGAGCTTCGAGCTCTCCAACCATTATAACGGGGTCGTTATAATGAACTCTCTAATCTCCATGTACGATAGGTGTGGTAAGTTACCCGATGCCGTCAAGGTGTTTGATGAAATGATCACAAGAGATACTATTTCGTGGAACGCATTGATTGGTGGGTTTGTGAGAAATGGGAAGTTTTTTGCTGGTTTTAGTTATTTCAAGGCTATGTGTTTAGTTGGTGATTGTAGATTTGACAAAGCTACTTTGACTACGATTTTATCTGCTTGTGATGGCTTGGAGTTTTGTTGGATTATTAAAATGATGCATGGTTTGGCGTTTTTGAGTGGGTATGGACAAGAAATTACTGTGGGGAATGCTCTGATTAGTTCGTATTTTAAATGTGGTTGTGTTGGTTTGGGGATGCAAGTTTTTTATGAGATGGGGGAGAGAAATGTGATTACTTGGACGGCTGTGATCTCCGGTTTAGCTCAAAATGGGTATCATGAGCACAGCCTGAAGCTGTTTAAGGAGATGATGAGTTATGGTTCTGTAGAGCCAAATTCTTTAACTTATTTGAGCTTACTCACTGCTTGTTCTGGTTTGGAGGCATTAAAGGAAGGATGTCAAATTCATGGCCTTATTATGAAGTTGGGAATTCAGTCAGATTTGTGCATTGGAAGTGCTCTAATGGATATGTACTCAAAATCTGGAAGAATTGGAGAGGCTTGGAAGATTTTCGAGTTGGCTGAGGAACTTGATATGGTTTCATTGACTGTTATACTTGCAGGGTTCACACATAATGGATGTGAGGAAGAAGCCATCCAGATCTTTCTGAAAATGTTGAAGATGGGGATTGAGATCGATGGAAATGTCGTTTCAGTTGTCCTTGGTGTGTTTGGTGCTGATACATCTTTGAGGCTGGGTCAACAAGTTCACTCATTCGTTGTCAAGAAGAACTTTATTTGCAACCCTTTTGTGAGCAATGGGCTTATAAACATGTACTCCAAGTGTGGAGCACTCGATGAATCAATGAAGGTCTTCGATAGGATGCGTGAAAGGAACTCGGTTACATGGAACTCCATGATTGCAGCGTTTGCCCGCCATGGAGATGCCTTGAAAGCTCTACAACTTTATGAGGATATGCAACTTGAAGGTGCAAAGCCAACTGACGTCACATTTCTATCATTACTTCATGCTTGTAGCCATGCCGGGTTAGTTAAAAAAGGAATGGAATTCCTCAAATCAATGACAAAAGATCACGGGATGAATCCAAGGAGCGAACACCATGCTTGTGTTGTTGACATGTTAGGTAGGGCAGGAATGCTGTCTGAAGCTAGAAACTTCATTGAGAAACTGCCTGAGCAGCCAGGTTTACTCGTGTGGCAGGCATTGCTCGGCGCCTGCAGCCTATATGGTGATTCTAAAATTGGCAAATATGCGGCCGAACATCTATTTTCGGAAACACCGGATAGTCCTGTCCCATATGTTTTGTTAGCCAACATATATTCTTCTGAAGGGAATTGGAAGGAAAGAGCAAGGACAATTAGGAAGATGAAGGAGGTGGGAACGGCCAAAGAAACTGGTATCAGCTGGATTGAGATTGACAAGAAAGTACACAGTTTTACTGTTGGAGACAAAATGCATCCACAAACTGAGATGATTTATGGAGTTTTGTGGGAGCTGTTTATACTCATGGTAGATGAAGGATATGTGCCAGATAAGAAGTTCATCCTCTACTACTTGGATGATGACAGGAGGGATCCAATCCATAACGGTCAAGCTACACATCAAAACGCCATAGAAACTGAAGTCGTTTGGGAGCTGTTTTGAAAACCAGTTACTTTTTATTATTATTCTTTTAAGCACGACCCGCTGGGTATAAAAATTTCAACTTCCAACTTCAAGTAAAGGAGGACATTCCCATTAGTTATACTCACCCCCACAAACACAATTTTGTATCTTATTATTGCTGC

mRNA sequence

ATGAAACTCAAATGGGTATTTCAAAAACGGAGCTCACACCTTCCCTCTTGGGTCACTTCTCTAATCTCCCCTTTCAGAAACCAATTCCATCAAAACCCATTTCCAGAAACCTCCTCAACATTCGTCCTCAACCATCTAGACCCAAGCTTCCTTCTATCCATTTGTGGAAGAGAAGGGAACCTCCATTTGGGTTCTTCTCTCCATGCATCCATCATCAAGAGCTTCGAGCTCTCCAACCATTATAACGGGGTCGTTATAATGAACTCTCTAATCTCCATGTACGATAGGTGTGGTAAGTTACCCGATGCCGTCAAGGTGTTTGATGAAATGATCACAAGAGATACTATTTCGTGGAACGCATTGATTGGTGGGTTTGTGAGAAATGGGAAGTTTTTTGCTGGTTTTAGTTATTTCAAGGCTATGTGTTTAGTTGGTGATTGTAGATTTGACAAAGCTACTTTGACTACGATTTTATCTGCTTGTGATGGCTTGGAGTTTTGTTGGATTATTAAAATGATGCATGGTTTGGCGTTTTTGAGTGGGTATGGACAAGAAATTACTGTGGGGAATGCTCTGATTAGTTCGTATTTTAAATGTGGTTGTGTTGGTTTGGGGATGCAAGTTTTTTATGAGATGGGGGAGAGAAATGTGATTACTTGGACGGCTGTGATCTCCGGTTTAGCTCAAAATGGGTATCATGAGCACAGCCTGAAGCTGTTTAAGGAGATGATGAGTTATGGTTCTGTAGAGCCAAATTCTTTAACTTATTTGAGCTTACTCACTGCTTGTTCTGGTTTGGAGGCATTAAAGGAAGGATGTCAAATTCATGGCCTTATTATGAAGTTGGGAATTCAGTCAGATTTGTGCATTGGAAGTGCTCTAATGGATATGTACTCAAAATCTGGAAGAATTGGAGAGGCTTGGAAGATTTTCGAGTTGGCTGAGGAACTTGATATGGTTTCATTGACTGTTATACTTGCAGGGTTCACACATAATGGATGTGAGGAAGAAGCCATCCAGATCTTTCTGAAAATGTTGAAGATGGGGATTGAGATCGATGGAAATGTCGTTTCAGTTGTCCTTGGTGTGTTTGGTGCTGATACATCTTTGAGGCTGGGTCAACAAGTTCACTCATTCGTTGTCAAGAAGAACTTTATTTGCAACCCTTTTGTGAGCAATGGGCTTATAAACATGTACTCCAAGTGTGGAGCACTCGATGAATCAATGAAGGTCTTCGATAGGATGCGTGAAAGGAACTCGGTTACATGGAACTCCATGATTGCAGCGTTTGCCCGCCATGGAGATGCCTTGAAAGCTCTACAACTTTATGAGGATATGCAACTTGAAGGTGCAAAGCCAACTGACGTCACATTTCTATCATTACTTCATGCTTGTAGCCATGCCGGGTTAGTTAAAAAAGGAATGGAATTCCTCAAATCAATGACAAAAGATCACGGGATGAATCCAAGGAGCGAACACCATGCTTGTGTTGTTGACATGTTAGGTAGGGCAGGAATGCTGTCTGAAGCTAGAAACTTCATTGAGAAACTGCCTGAGCAGCCAGGTTTACTCGTGTGGCAGGCATTGCTCGGCGCCTGCAGCCTATATGGTGATTCTAAAATTGGCAAATATGCGGCCGAACATCTATTTTCGGAAACACCGGATAGTCCTGTCCCATATGTTTTGTTAGCCAACATATATTCTTCTGAAGGGAATTGGAAGGAAAGAGCAAGGACAATTAGGAAGATGAAGGAGGTGGGAACGGCCAAAGAAACTGGTATCAGCTGGATTGAGATTGACAAGAAAGTACACAGTTTTACTGTTGGAGACAAAATGCATCCACAAACTGAGATGATTTATGGAGTTTTGTGGGAGCTGTTTATACTCATGGTAGATGAAGGATATGTGCCAGATAAGAAGTTCATCCTCTACTACTTGGATGATGACAGGAGGGATCCAATCCATAACGGTCAAGCTACACATCAAAACGCCATAGAAACTGAAGTCGTTTGGGAGCTGTTTTGA

Coding sequence (CDS)

ATGAAACTCAAATGGGTATTTCAAAAACGGAGCTCACACCTTCCCTCTTGGGTCACTTCTCTAATCTCCCCTTTCAGAAACCAATTCCATCAAAACCCATTTCCAGAAACCTCCTCAACATTCGTCCTCAACCATCTAGACCCAAGCTTCCTTCTATCCATTTGTGGAAGAGAAGGGAACCTCCATTTGGGTTCTTCTCTCCATGCATCCATCATCAAGAGCTTCGAGCTCTCCAACCATTATAACGGGGTCGTTATAATGAACTCTCTAATCTCCATGTACGATAGGTGTGGTAAGTTACCCGATGCCGTCAAGGTGTTTGATGAAATGATCACAAGAGATACTATTTCGTGGAACGCATTGATTGGTGGGTTTGTGAGAAATGGGAAGTTTTTTGCTGGTTTTAGTTATTTCAAGGCTATGTGTTTAGTTGGTGATTGTAGATTTGACAAAGCTACTTTGACTACGATTTTATCTGCTTGTGATGGCTTGGAGTTTTGTTGGATTATTAAAATGATGCATGGTTTGGCGTTTTTGAGTGGGTATGGACAAGAAATTACTGTGGGGAATGCTCTGATTAGTTCGTATTTTAAATGTGGTTGTGTTGGTTTGGGGATGCAAGTTTTTTATGAGATGGGGGAGAGAAATGTGATTACTTGGACGGCTGTGATCTCCGGTTTAGCTCAAAATGGGTATCATGAGCACAGCCTGAAGCTGTTTAAGGAGATGATGAGTTATGGTTCTGTAGAGCCAAATTCTTTAACTTATTTGAGCTTACTCACTGCTTGTTCTGGTTTGGAGGCATTAAAGGAAGGATGTCAAATTCATGGCCTTATTATGAAGTTGGGAATTCAGTCAGATTTGTGCATTGGAAGTGCTCTAATGGATATGTACTCAAAATCTGGAAGAATTGGAGAGGCTTGGAAGATTTTCGAGTTGGCTGAGGAACTTGATATGGTTTCATTGACTGTTATACTTGCAGGGTTCACACATAATGGATGTGAGGAAGAAGCCATCCAGATCTTTCTGAAAATGTTGAAGATGGGGATTGAGATCGATGGAAATGTCGTTTCAGTTGTCCTTGGTGTGTTTGGTGCTGATACATCTTTGAGGCTGGGTCAACAAGTTCACTCATTCGTTGTCAAGAAGAACTTTATTTGCAACCCTTTTGTGAGCAATGGGCTTATAAACATGTACTCCAAGTGTGGAGCACTCGATGAATCAATGAAGGTCTTCGATAGGATGCGTGAAAGGAACTCGGTTACATGGAACTCCATGATTGCAGCGTTTGCCCGCCATGGAGATGCCTTGAAAGCTCTACAACTTTATGAGGATATGCAACTTGAAGGTGCAAAGCCAACTGACGTCACATTTCTATCATTACTTCATGCTTGTAGCCATGCCGGGTTAGTTAAAAAAGGAATGGAATTCCTCAAATCAATGACAAAAGATCACGGGATGAATCCAAGGAGCGAACACCATGCTTGTGTTGTTGACATGTTAGGTAGGGCAGGAATGCTGTCTGAAGCTAGAAACTTCATTGAGAAACTGCCTGAGCAGCCAGGTTTACTCGTGTGGCAGGCATTGCTCGGCGCCTGCAGCCTATATGGTGATTCTAAAATTGGCAAATATGCGGCCGAACATCTATTTTCGGAAACACCGGATAGTCCTGTCCCATATGTTTTGTTAGCCAACATATATTCTTCTGAAGGGAATTGGAAGGAAAGAGCAAGGACAATTAGGAAGATGAAGGAGGTGGGAACGGCCAAAGAAACTGGTATCAGCTGGATTGAGATTGACAAGAAAGTACACAGTTTTACTGTTGGAGACAAAATGCATCCACAAACTGAGATGATTTATGGAGTTTTGTGGGAGCTGTTTATACTCATGGTAGATGAAGGATATGTGCCAGATAAGAAGTTCATCCTCTACTACTTGGATGATGACAGGAGGGATCCAATCCATAACGGTCAAGCTACACATCAAAACGCCATAGAAACTGAAGTCGTTTGGGAGCTGTTTTGA

Protein sequence

MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGNLHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGQATHQNAIETEVVWELF*
BLAST of Csa2G022820 vs. Swiss-Prot
Match: PP215_ARATH (Pentatricopeptide repeat-containing protein At3g05340 OS=Arabidopsis thaliana GN=PCMP-E83 PE=2 SV=2)

HSP 1 Score: 748.4 bits (1931), Expect = 6.7e-215
Identity = 388/661 (58.70%), Postives = 473/661 (71.56%), Query Frame = 1

Query: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN 60
           M  +WV QK +SHLPS +++++SP +    Q+P  + S TF+LNH+D S LLSICGREG 
Sbjct: 1   MNSRWVIQKLTSHLPSCLSTVLSPSKILIRQSPNYQVS-TFLLNHVDMSLLLSICGREGW 60

Query: 61  L-HLGSSLHASIIKSFELSN------HYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITR 120
             HLG  LHASIIK+ E         H N +V+ NSL+S+Y +CGKL DA+K+FDEM  R
Sbjct: 61  FPHLGPCLHASIIKNPEFFEPVDADIHRNALVVWNSLLSLYAKCGKLVDAIKLFDEMPMR 120

Query: 121 DTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMM 180
           D IS N +  GF+RN +  +GF   K M  +G   FD ATLT +LS CD  EFC + KM+
Sbjct: 121 DVISQNIVFYGFLRNRETESGFVLLKRM--LGSGGFDHATLTIVLSVCDTPEFCLVTKMI 180

Query: 181 HGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYH 240
           H LA LSGY +EI+VGN LI+SYFKCGC   G  VF  M  RNVIT TAVISGL +N  H
Sbjct: 181 HALAILSGYDKEISVGNKLITSYFKCGCSVSGRGVFDGMSHRNVITLTAVISGLIENELH 240

Query: 241 EHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSA 300
           E  L+LF  +M  G V PNS+TYLS L ACSG + + EG QIH L+ K GI+S+LCI SA
Sbjct: 241 EDGLRLFS-LMRRGLVHPNSVTYLSALAACSGSQRIVEGQQIHALLWKYGIESELCIESA 300

Query: 301 LMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEID 360
           LMDMYSK G I +AW IFE   E+D VS+TVIL G   NG EEEAIQ F++ML+ G+EID
Sbjct: 301 LMDMYSKCGSIEDAWTIFESTTEVDEVSMTVILVGLAQNGSEEEAIQFFIRMLQAGVEID 360

Query: 361 GNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFD 420
            NVVS VLGV   D SL LG+Q+HS V+K+ F  N FV+NGLINMYSKCG L +S  VF 
Sbjct: 361 ANVVSAVLGVSFIDNSLGLGKQLHSLVIKRKFSGNTFVNNGLINMYSKCGDLTDSQTVFR 420

Query: 421 RMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKK 480
           RM +RN V+WNSMIAAFARHG  L AL+LYE+M     KPTDVTFLSLLHACSH GL+ K
Sbjct: 421 RMPKRNYVSWNSMIAAFARHGHGLAALKLYEEMTTLEVKPTDVTFLSLLHACSHVGLIDK 480

Query: 481 GMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGAC 540
           G E L  M + HG+ PR+EH+ C++DMLGRAG+L EA++FI+ LP +P   +WQALLGAC
Sbjct: 481 GRELLNEMKEVHGIEPRTEHYTCIIDMLGRAGLLKEAKSFIDSLPLKPDCKIWQALLGAC 540

Query: 541 SLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETG 600
           S +GD+++G+YAAE LF   PDS   ++L+ANIYSS G WKERA+TI++MK +G  KETG
Sbjct: 541 SFHGDTEVGEYAAEQLFQTAPDSSSAHILIANIYSSRGKWKERAKTIKRMKAMGVTKETG 600

Query: 601 ISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDP 655
           IS IEI+ K HSF V DK+HPQ E IY VL  LF +MVDEGY PDK+FIL Y  DDR   
Sbjct: 601 ISSIEIEHKTHSFVVEDKLHPQAEAIYDVLSGLFPVMVDEGYRPDKRFILCYTGDDRNGT 657

BLAST of Csa2G022820 vs. Swiss-Prot
Match: PP296_ARATH (Pentatricopeptide repeat-containing protein At3g63370, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H83 PE=2 SV=2)

HSP 1 Score: 387.1 bits (993), Expect = 3.9e-106
Identity = 207/609 (33.99%), Postives = 349/609 (57.31%), Query Frame = 1

Query: 52  LSICGREGNLHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMI 111
           L+ C       LG  +HAS++KS   S H + + + N+LI+MY RCGK+P A ++  +M 
Sbjct: 291 LTACDGFSYAKLGKEIHASVLKS---STHSSELYVCNALIAMYTRCGKMPQAERILRQMN 350

Query: 112 TRDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIK 171
             D ++WN+LI G+V+N  +     +F  M   G  + D+ ++T+I++A   L       
Sbjct: 351 NADVVTWNSLIKGYVQNLMYKEALEFFSDMIAAGH-KSDEVSMTSIIAASGRLSNLLAGM 410

Query: 172 MMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNG 231
            +H      G+   + VGN LI  Y KC       + F  M ++++I+WT VI+G AQN 
Sbjct: 411 ELHAYVIKHGWDSNLQVGNTLIDMYSKCNLTCYMGRAFLRMHDKDLISWTTVIAGYAQND 470

Query: 232 YHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIG 291
            H  +L+LF+++     +E + +   S+L A S L+++    +IH  I++ G+  D  I 
Sbjct: 471 CHVEALELFRDVAKK-RMEIDEMILGSILRASSVLKSMLIVKEIHCHILRKGLL-DTVIQ 530

Query: 292 SALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIE 351
           + L+D+Y K   +G A ++FE  +  D+VS T +++    NG E EA+++F +M++ G+ 
Sbjct: 531 NELVDVYGKCRNMGYATRVFESIKGKDVVSWTSMISSSALNGNESEAVELFRRMVETGLS 590

Query: 352 IDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKV 411
            D   +  +L    + ++L  G+++H ++++K F     ++  +++MY+ CG L  +  V
Sbjct: 591 ADSVALLCILSAAASLSALNKGREIHCYLLRKGFCLEGSIAVAVVDMYACCGDLQSAKAV 650

Query: 412 FDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLV 471
           FDR+  +  + + SMI A+  HG    A++L++ M+ E   P  ++FL+LL+ACSHAGL+
Sbjct: 651 FDRIERKGLLQYTSMINAYGMHGCGKAAVELFDKMRHENVSPDHISFLALLYACSHAGLL 710

Query: 472 KKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLG 531
            +G  FLK M  ++ + P  EH+ C+VDMLGRA  + EA  F++ +  +P   VW ALL 
Sbjct: 711 DEGRGFLKIMEHEYELEPWPEHYVCLVDMLGRANCVVEAFEFVKMMKTEPTAEVWCALLA 770

Query: 532 ACSLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKE 591
           AC  + + +IG+ AA+ L    P +P   VL++N+++ +G W +  +   KMK  G  K 
Sbjct: 771 ACRSHSEKEIGEIAAQRLLELEPKNPGNLVLVSNVFAEQGRWNDVEKVRAKMKASGMEKH 830

Query: 592 TGISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDE-GYVPDKKFILYYLDDDR 651
            G SWIE+D KVH FT  DK HP+++ IY  L E+   +  E GYV D KF+L+ +D+  
Sbjct: 831 PGCSWIEMDGKVHKFTARDKSHPESKEIYEKLSEVTRKLEREVGYVADTKFVLHNVDEGE 890

Query: 652 RDPIHNGQA 660
           +  + +G +
Sbjct: 891 KVQMLHGHS 893


HSP 2 Score: 225.3 bits (573), Expect = 2.0e-57
Identity = 147/541 (27.17%), Postives = 274/541 (50.65%), Query Frame = 1

Query: 51  LLSICGREGNLHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEM 110
           LL  C +  ++  GS LH+ ++K   L  H  G ++ N+L+SMY +   L  A ++FD  
Sbjct: 188 LLKACAKLRDIRSGSELHSLLVK---LGYHSTGFIV-NALVSMYAKNDDLSAARRLFDGF 247

Query: 111 ITR-DTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWI 170
             + D + WN+++  +  +GK       F+ M + G    +  T+ + L+ACDG  +  +
Sbjct: 248 QEKGDAVLWNSILSSYSTSGKSLETLELFREMHMTGPAP-NSYTIVSALTACDGFSYAKL 307

Query: 171 IKMMHGLAFLSG-YGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLA 230
            K +H     S  +  E+ V NALI+ Y +CG +    ++  +M   +V+TW ++I G  
Sbjct: 308 GKEIHASVLKSSTHSSELYVCNALIAMYTRCGKMPQAERILRQMNNADVVTWNSLIKGYV 367

Query: 231 QNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDL 290
           QN  ++ +L+ F +M++ G  + + ++  S++ A   L  L  G ++H  ++K G  S+L
Sbjct: 368 QNLMYKEALEFFSDMIAAGH-KSDEVSMTSIIAASGRLSNLLAGMELHAYVIKHGWDSNL 427

Query: 291 CIGSALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKM 350
            +G+ L+DMYSK        + F    + D++S T ++AG+  N C  EA+++F  + K 
Sbjct: 428 QVGNTLIDMYSKCNLTCYMGRAFLRMHDKDLISWTTVIAGYAQNDCHVEALELFRDVAKK 487

Query: 351 GIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDES 410
            +EID  ++  +L       S+ + +++H  +++K  + +  + N L+++Y KC  +  +
Sbjct: 488 RMEIDEMILGSILRASSVLKSMLIVKEIHCHILRKGLL-DTVIQNELVDVYGKCRNMGYA 547

Query: 411 MKVFDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHA 470
            +VF+ ++ ++ V+W SMI++ A +G+  +A++L+  M   G     V  L +L A +  
Sbjct: 548 TRVFESIKGKDVVSWTSMISSSALNGNESEAVELFRRMVETGLSADSVALLCILSAAASL 607

Query: 471 GLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQA 530
             + KG E    + +  G          VVDM    G L  A+   +++ E+ GLL + +
Sbjct: 608 SALNKGREIHCYLLR-KGFCLEGSIAVAVVDMYACCGDLQSAKAVFDRI-ERKGLLQYTS 667

Query: 531 LLGACSLYGDSKIGKYAAEHLFSETPD---SPVPYVLLANIYS-SEGNWKERARTIRKMK 586
           ++ A  ++G  K    AA  LF +      SP     LA +Y+ S     +  R   K+ 
Sbjct: 668 MINAYGMHGCGK----AAVELFDKMRHENVSPDHISFLALLYACSHAGLLDEGRGFLKIM 715


HSP 3 Score: 204.5 bits (519), Expect = 3.6e-51
Identity = 138/506 (27.27%), Postives = 254/506 (50.20%), Query Frame = 1

Query: 49  SFLLSICGREGNLHLGSSLHASIIK---SFELSNHYNGVVIMNSLISMYDRCGKLPDAVK 108
           +++L +CG+   +  G  LH+ I K   SFEL        +   L+ MY +CG L DA K
Sbjct: 84  AYVLELCGKRRAVSQGRQLHSRIFKTFPSFELD------FLAGKLVFMYGKCGSLDDAEK 143

Query: 109 VFDEMITRDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLE 168
           VFDEM  R   +WN +IG +V NG+  +  + +  M + G      ++   +L AC  L 
Sbjct: 144 VFDEMPDRTAFAWNTMIGAYVSNGEPASALALYWNMRVEG-VPLGLSSFPALLKACAKLR 203

Query: 169 FCWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGER-NVITWTAVI 228
                  +H L    GY     + NAL+S Y K   +    ++F    E+ + + W +++
Sbjct: 204 DIRSGSELHSLLVKLGYHSTGFIVNALVSMYAKNDDLSAARRLFDGFQEKGDAVLWNSIL 263

Query: 229 SGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGI 288
           S  + +G    +L+LF+EM   G   PNS T +S LTAC G    K G +IH  ++K   
Sbjct: 264 SSYSTSGKSLETLELFREMHMTGPA-PNSYTIVSALTACDGFSYAKLGKEIHASVLKSST 323

Query: 289 QS-DLCIGSALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFL 348
            S +L + +AL+ MY++ G++ +A +I       D+V+   ++ G+  N   +EA++ F 
Sbjct: 324 HSSELYVCNALIAMYTRCGKMPQAERILRQMNNADVVTWNSLIKGYVQNLMYKEALEFFS 383

Query: 349 KMLKMGIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCG 408
            M+  G + D   ++ ++   G  ++L  G ++H++V+K  +  N  V N LI+MYSKC 
Sbjct: 384 DMIAAGHKSDEVSMTSIIAASGRLSNLLAGMELHAYVIKHGWDSNLQVGNTLIDMYSKCN 443

Query: 409 ALDESMKVFDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLH 468
                 + F RM +++ ++W ++IA +A++   ++AL+L+ D+  +  +  ++   S+L 
Sbjct: 444 LTCYMGRAFLRMHDKDLISWTTVIAGYAQNDCHVEALELFRDVAKKRMEIDEMILGSILR 503

Query: 469 ACS-----------HAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARN 528
           A S           H  +++KG+  L ++ ++            +VD+ G+   +  A  
Sbjct: 504 ASSVLKSMLIVKEIHCHILRKGL--LDTVIQNE-----------LVDVYGKCRNMGYATR 563

Query: 529 FIEKLPEQPGLLVWQALLGACSLYGD 539
             E + +   ++ W +++ + +L G+
Sbjct: 564 VFESI-KGKDVVSWTSMISSSALNGN 567


HSP 4 Score: 94.0 bits (232), Expect = 6.8e-18
Identity = 64/216 (29.63%), Postives = 111/216 (51.39%), Query Frame = 1

Query: 360 VLGVFGADTSLRLGQQVHSFVVKK--NFICNPFVSNGLINMYSKCGALDESMKVFDRMRE 419
           VL + G   ++  G+Q+HS + K   +F  + F++  L+ MY KCG+LD++ KVFD M +
Sbjct: 86  VLELCGKRRAVSQGRQLHSRIFKTFPSFELD-FLAGKLVFMYGKCGSLDDAEKVFDEMPD 145

Query: 420 RNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEF 479
           R +  WN+MI A+  +G+   AL LY +M++EG      +F +LL AC+    ++ G E 
Sbjct: 146 RTAFAWNTMIGAYVSNGEPASALALYWNMRVEGVPLGLSSFPALLKACAKLRDIRSGSE- 205

Query: 480 LKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYG 539
           L S+    G +        +V M  +   LS AR   +   E+   ++W ++L + S  G
Sbjct: 206 LHSLLVKLGYHSTGFIVNALVSMYAKNDDLSAARRLFDGFQEKGDAVLWNSILSSYSTSG 265

Query: 540 DS--KIGKYAAEHLFSETPDSPVPYVLLANIYSSEG 572
            S   +  +   H+    P+S   Y +++ + + +G
Sbjct: 266 KSLETLELFREMHMTGPAPNS---YTIVSALTACDG 296

BLAST of Csa2G022820 vs. Swiss-Prot
Match: PP373_ARATH (Putative pentatricopeptide repeat-containing protein At5g09950 OS=Arabidopsis thaliana GN=PCMP-H35 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 5.1e-106
Identity = 217/579 (37.48%), Postives = 336/579 (58.03%), Query Frame = 1

Query: 84  VVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALIGGFVRNGKFFAGFSYFKAMCL 143
           V I N L++MY +CG + DA +VF  M  +D++SWN++I G  +NG F      +K+M  
Sbjct: 349 VGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVERYKSMRR 408

Query: 144 VGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVG 203
             D      TL + LS+C  L++  + + +HG +   G    ++V NAL++ Y + G + 
Sbjct: 409 -HDILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVSVSNALMTLYAETGYLN 468

Query: 204 LGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLK----LFKEMMSYGSVEPNSLTYLSL 263
              ++F  M E + ++W ++I  LA++   E SL      F      G  + N +T+ S+
Sbjct: 469 ECRKIFSSMPEHDQVSWNSIIGALARS---ERSLPEAVVCFLNAQRAGQ-KLNRITFSSV 528

Query: 264 LTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIFE-LAEELD 323
           L+A S L   + G QIHGL +K  I  +    +AL+  Y K G +    KIF  +AE  D
Sbjct: 529 LSAVSSLSFGELGKQIHGLALKNNIADEATTENALIACYGKCGEMDGCEKIFSRMAERRD 588

Query: 324 MVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVFGADTSLRLGQQVHS 383
            V+   +++G+ HN    +A+ +   ML+ G  +D  + + VL  F +  +L  G +VH+
Sbjct: 589 NVTWNSMISGYIHNELLAKALDLVWFMLQTGQRLDSFMYATVLSAFASVATLERGMEVHA 648

Query: 384 FVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNSVTWNSMIAAFARHGDALK 443
             V+     +  V + L++MYSKCG LD +++ F+ M  RNS +WNSMI+ +ARHG   +
Sbjct: 649 CSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVRNSYSWNSMISGYARHGQGEE 708

Query: 444 ALQLYEDMQLEGAKPTD-VTFLSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSEHHACV 503
           AL+L+E M+L+G  P D VTF+ +L ACSHAGL+++G +  +SM+  +G+ PR EH +C+
Sbjct: 709 ALKLFETMKLDGQTPPDHVTFVGVLSACSHAGLLEEGFKHFESMSDSYGLAPRIEHFSCM 768

Query: 504 VDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK--IGKYAAEHLFSETPD 563
            D+LGRAG L +  +FIEK+P +P +L+W+ +LGAC      K  +GK AAE LF   P+
Sbjct: 769 ADVLGRAGELDKLEDFIEKMPMKPNVLIWRTVLGACCRANGRKAELGKKAAEMLFQLEPE 828

Query: 564 SPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEIDKKVHSFTVGDKMHPQ 623
           + V YVLL N+Y++ G W++  +  +KMK+    KE G SW+ +   VH F  GDK HP 
Sbjct: 829 NAVNYVLLGNMYAAGGRWEDLVKARKKMKDADVKKEAGYSWVTMKDGVHMFVAGDKSHPD 888

Query: 624 TEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPI 655
            ++IY  L EL   M D GYVP   F LY L+ + ++ I
Sbjct: 889 ADVIYKKLKELNRKMRDAGYVPQTGFALYDLEQENKEEI 922


HSP 2 Score: 205.7 bits (522), Expect = 1.6e-51
Identity = 137/460 (29.78%), Postives = 232/460 (50.43%), Query Frame = 1

Query: 84  VVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALIGGFVRNGKFFAGFSYFKAMCL 143
           V + N+LI+ Y   G    A KVFDEM  R+ +SW  ++ G+ RNG+      + + M  
Sbjct: 36  VYLCNNLINAYLETGDSVSARKVFDEMPLRNCVSWACIVSGYSRNGEHKEALVFLRDMVK 95

Query: 144 VGDCRFDKATLTTILSACDGLEFCWII--KMMHGLAFLSGYGQEITVGNALISSYFKC-G 203
            G    ++    ++L AC  +    I+  + +HGL F   Y  +  V N LIS Y+KC G
Sbjct: 96  EGIFS-NQYAFVSVLRACQEIGSVGILFGRQIHGLMFKLSYAVDAVVSNVLISMYWKCIG 155

Query: 204 CVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLL 263
            VG  +  F ++  +N ++W ++IS  +Q G    + ++F  M   GS  P   T+ SL+
Sbjct: 156 SVGYALCAFGDIEVKNSVSWNSIISVYSQAGDQRSAFRIFSSMQYDGS-RPTEYTFGSLV 215

Query: 264 T-ACSGLEA-LKEGCQIHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIFELAEELD 323
           T ACS  E  ++   QI   I K G+ +DL +GS L+  ++KSG +  A K+F   E  +
Sbjct: 216 TTACSLTEPDVRLLEQIMCTIQKSGLLTDLFVGSGLVSAFAKSGSLSYARKVFNQMETRN 275

Query: 324 MVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVF-----GADTSLRLG 383
            V+L  ++ G       EEA ++F+ M  M I++      ++L  F       +  L+ G
Sbjct: 276 AVTLNGLMVGLVRQKWGEEATKLFMDMNSM-IDVSPESYVILLSSFPEYSLAEEVGLKKG 335

Query: 384 QQVHSFVVKKNFICNPF-VSNGLINMYSKCGALDESMKVFDRMRERNSVTWNSMIAAFAR 443
           ++VH  V+    +     + NGL+NMY+KCG++ ++ +VF  M +++SV+WNSMI    +
Sbjct: 336 REVHGHVITTGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQ 395

Query: 444 HGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSE 503
           +G  ++A++ Y+ M+     P   T +S L +C+     K G +      K  G++    
Sbjct: 396 NGCFIEAVERYKSMRRHDILPGSFTLISSLSSCASLKWAKLGQQIHGESLK-LGIDLNVS 455

Query: 504 HHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGA 533
               ++ +    G L+E R     +PE    + W +++GA
Sbjct: 456 VSNALMTLYAETGYLNECRKIFSSMPEH-DQVSWNSIIGA 490


HSP 3 Score: 193.7 bits (491), Expect = 6.3e-48
Identity = 147/538 (27.32%), Postives = 257/538 (47.77%), Query Frame = 1

Query: 58  EGNLHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTIS 117
           E ++ L   +  +I KS  L++ + G    + L+S + + G L  A KVF++M TR+ ++
Sbjct: 221 EPDVRLLEQIMCTIQKSGLLTDLFVG----SGLVSAFAKSGSLSYARKVFNQMETRNAVT 280

Query: 118 WNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKM----- 177
            N L+ G VR          F  M  + D   +   +  +LS+         + +     
Sbjct: 281 LNGLMVGLVRQKWGEEATKLFMDMNSMIDVSPESYVI--LLSSFPEYSLAEEVGLKKGRE 340

Query: 178 MHGLAFLSGYGQ-EITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNG 237
           +HG    +G     + +GN L++ Y KCG +    +VFY M +++ ++W ++I+GL QNG
Sbjct: 341 VHGHVITTGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQNG 400

Query: 238 YHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIG 297
               +++ +K M  +  + P S T +S L++C+ L+  K G QIHG  +KLGI  ++ + 
Sbjct: 401 CFIEAVERYKSMRRH-DILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVSVS 460

Query: 298 SALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCE-EEAIQIFLKMLKMGI 357
           +ALM +Y+++G + E  KIF    E D VS   I+     +     EA+  FL   + G 
Sbjct: 461 NALMTLYAETGYLNECRKIFSSMPEHDQVSWNSIIGALARSERSLPEAVVCFLNAQRAGQ 520

Query: 358 EIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMK 417
           +++    S VL    + +   LG+Q+H   +K N        N LI  Y KCG +D   K
Sbjct: 521 KLNRITFSSVLSAVSSLSFGELGKQIHGLALKNNIADEATTENALIACYGKCGEMDGCEK 580

Query: 418 VFDRMRE-RNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAG 477
           +F RM E R++VTWNSMI+ +  +    KAL L   M   G +     + ++L A +   
Sbjct: 581 IFSRMAERRDNVTWNSMISGYIHNELLAKALDLVWFMLQTGQRLDSFMYATVLSAFASVA 640

Query: 478 LVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQAL 537
            +++GME + + +    +       + +VDM  + G L  A  F   +P +     W ++
Sbjct: 641 TLERGME-VHACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVR-NSYSWNSM 700

Query: 538 LGACSLY--GDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKE 586
           +   + +  G+  +  +    L  +TP   V +V + +  S  G  +E  +    M +
Sbjct: 701 ISGYARHGQGEEALKLFETMKLDGQTPPDHVTFVGVLSACSHAGLLEEGFKHFESMSD 749


HSP 4 Score: 175.6 bits (444), Expect = 1.8e-42
Identity = 133/491 (27.09%), Postives = 241/491 (49.08%), Query Frame = 1

Query: 51  LLSICGREGNLHL--GSSLHASIIKSFELSNHYNGVVIMNSLISMYDRC-GKLPDAVKVF 110
           +L  C   G++ +  G  +H  +   F+LS   + VV  N LISMY +C G +  A+  F
Sbjct: 108 VLRACQEIGSVGILFGRQIHGLM---FKLSYAVDAVV-SNVLISMYWKCIGSVGYALCAF 167

Query: 111 DEMITRDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLE-F 170
            ++  ++++SWN++I  + + G   + F  F +M   G    +    + + +AC   E  
Sbjct: 168 GDIEVKNSVSWNSIISVYSQAGDQRSAFRIFSSMQYDGSRPTEYTFGSLVTTACSLTEPD 227

Query: 171 CWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISG 230
             +++ +      SG   ++ VG+ L+S++ K G +    +VF +M  RN +T   ++ G
Sbjct: 228 VRLLEQIMCTIQKSGLLTDLFVGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVG 287

Query: 231 LAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLE-----ALKEGCQIHGLIMK 290
           L +  + E + KLF +M S   V P S  Y+ LL++           LK+G ++HG ++ 
Sbjct: 288 LVRQKWGEEATKLFMDMNSMIDVSPES--YVILLSSFPEYSLAEEVGLKKGREVHGHVIT 347

Query: 291 LGIQSDLC-IGSALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQ 350
            G+   +  IG+ L++MY+K G I +A ++F    + D VS   ++ G   NGC  EA++
Sbjct: 348 TGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVE 407

Query: 351 IFLKMLKMGIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYS 410
            +  M +  I      +   L    +    +LGQQ+H   +K     N  VSN L+ +Y+
Sbjct: 408 RYKSMRRHDILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVSVSNALMTLYA 467

Query: 411 KCGALDESMKVFDRMRERNSVTWNSMIAAFARHGDAL-KALQLYEDMQLEGAKPTDVTFL 470
           + G L+E  K+F  M E + V+WNS+I A AR   +L +A+  + + Q  G K   +TF 
Sbjct: 468 ETGYLNECRKIFSSMPEHDQVSWNSIIGALARSERSLPEAVVCFLNAQRAGQKLNRITFS 527

Query: 471 SLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPE 530
           S+L A S     + G +      K++  +  +  +A ++   G+ G +        ++ E
Sbjct: 528 SVLSAVSSLSFGELGKQIHGLALKNNIADEATTENA-LIACYGKCGEMDGCEKIFSRMAE 587


HSP 5 Score: 115.9 bits (289), Expect = 1.7e-24
Identity = 92/327 (28.13%), Postives = 152/327 (46.48%), Query Frame = 1

Query: 43  LNHLDPSFLLSICGREGNLHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPD 102
           LN +  S +LS         LG  +H   +K+    N  +     N+LI+ Y +CG++  
Sbjct: 515 LNRITFSSVLSAVSSLSFGELGKQIHGLALKN----NIADEATTENALIACYGKCGEMDG 574

Query: 103 AVKVFDEMITR-DTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSAC 162
             K+F  M  R D ++WN++I G++ N            M   G  R D     T+LSA 
Sbjct: 575 CEKIFSRMAERRDNVTWNSMISGYIHNELLAKALDLVWFMLQTGQ-RLDSFMYATVLSAF 634

Query: 163 DGLEFCWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWT 222
             +        +H  +  +    ++ VG+AL+  Y KCG +   ++ F  M  RN  +W 
Sbjct: 635 ASVATLERGMEVHACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVRNSYSWN 694

Query: 223 AVISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQ-IHGLIM 282
           ++ISG A++G  E +LKLF+ M   G   P+ +T++ +L+ACS    L+EG +    +  
Sbjct: 695 SMISGYARHGQGEEALKLFETMKLDGQTPPDHVTFVGVLSACSHAGLLEEGFKHFESMSD 754

Query: 283 KLGIQSDLCIGSALMDMYSKSGRIGEAWKIFELAEELDM-----VSLTVILAGFTHNGCE 342
             G+   +   S + D+    GR GE  K+ +  E++ M     +  TV+ A    NG +
Sbjct: 755 SYGLAPRIEHFSCMADVL---GRAGELDKLEDFIEKMPMKPNVLIWRTVLGACCRANGRK 814

Query: 343 EEAIQIFLKMLKMGIEIDGNVVSVVLG 363
            E  +   +ML   +E +  V  V+LG
Sbjct: 815 AELGKKAAEML-FQLEPENAVNYVLLG 832


HSP 6 Score: 102.4 bits (254), Expect = 1.9e-20
Identity = 94/399 (23.56%), Postives = 179/399 (44.86%), Query Frame = 1

Query: 257 LSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIFELAEE 316
           LS + +C G          H  + K  +  D+ + + L++ Y ++G    A K+F+    
Sbjct: 7   LSFVQSCVGHRGAARF--FHSRLYKNRLDKDVYLCNNLINAYLETGDSVSARKVFDEMPL 66

Query: 317 LDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVFG--ADTSLRLGQ 376
            + VS   I++G++ NG  +EA+     M+K GI  +      VL          +  G+
Sbjct: 67  RNCVSWACIVSGYSRNGEHKEALVFLRDMVKEGIFSNQYAFVSVLRACQEIGSVGILFGR 126

Query: 377 QVHSFVVKKNFICNPFVSNGLINMYSKC-GALDESMKVFDRMRERNSVTWNSMIAAFARH 436
           Q+H  + K ++  +  VSN LI+MY KC G++  ++  F  +  +NSV+WNS+I+ +++ 
Sbjct: 127 QIHGLMFKLSYAVDAVVSNVLISMYWKCIGSVGYALCAFGDIEVKNSVSWNSIISVYSQA 186

Query: 437 GDALKALQLYEDMQLEGAKPTDVTFLSLL-HACSHAGLVKKGMEFLKSMTKDHGMNPRSE 496
           GD   A +++  MQ +G++PT+ TF SL+  ACS      + +E +    +  G+     
Sbjct: 187 GDQRSAFRIFSSMQYDGSRPTEYTFGSLVTTACSLTEPDVRLLEQIMCTIQKSGLLTDLF 246

Query: 497 HHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSKIGKYAAE---HL 556
             + +V    ++G LS AR    ++  +  + +   ++G        K G+ A +    +
Sbjct: 247 VGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVGLVR----QKWGEEATKLFMDM 306

Query: 557 FSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEIDKKVHSFTVG 616
            S    SP  YV+L                +    E   A+E G   ++  ++VH   + 
Sbjct: 307 NSMIDVSPESYVIL----------------LSSFPEYSLAEEVG---LKKGREVHGHVIT 366

Query: 617 DKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDD 649
             +    + + G+   L  +    G + D + + Y++ D
Sbjct: 367 TGL---VDFMVGIGNGLVNMYAKCGSIADARRVFYFMTD 377

BLAST of Csa2G022820 vs. Swiss-Prot
Match: PP272_ARATH (Pentatricopeptide repeat-containing protein At3g49170, chloroplastic OS=Arabidopsis thaliana GN=EMB2261 PE=2 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 2.8e-104
Identity = 215/602 (35.71%), Postives = 334/602 (55.48%), Query Frame = 1

Query: 78  SNHYNGVVIMN-SLISMYDRC-GKLPDAVKVFDEMITRDTISWNALIGGFVRNGKFFAGF 137
           + H+   V +  SLI M+ +      +A KVFD+M   + ++W  +I   ++ G      
Sbjct: 195 TGHFESDVCVGCSLIDMFVKGENSFENAYKVFDKMSELNVVTWTLMITRCMQMGFPREAI 254

Query: 138 SYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLSGYGQEITVGNALISS 197
            +F  M L G    DK TL+++ SAC  LE   + K +H  A  SG   ++    +L+  
Sbjct: 255 RFFLDMVLSG-FESDKFTLSSVFSACAELENLSLGKQLHSWAIRSGLVDDVEC--SLVDM 314

Query: 198 YFKC---GCVGLGMQVFYEMGERNVITWTAVISGLAQN-GYHEHSLKLFKEMMSYGSVEP 257
           Y KC   G V    +VF  M + +V++WTA+I+G  +N      ++ LF EM++ G VEP
Sbjct: 315 YAKCSADGSVDDCRKVFDRMEDHSVMSWTALITGYMKNCNLATEAINLFSEMITQGHVEP 374

Query: 258 NSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIF 317
           N  T+ S   AC  L   + G Q+ G   K G+ S+  + ++++ M+ KS R+ +A + F
Sbjct: 375 NHFTFSSAFKACGNLSDPRVGKQVLGQAFKRGLASNSSVANSVISMFVKSDRMEDAQRAF 434

Query: 318 ELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVFGADTSLR 377
           E   E ++VS    L G   N   E+A ++  ++ +  + +     + +L       S+R
Sbjct: 435 ESLSEKNLVSYNTFLDGTCRNLNFEQAFKLLSEITERELGVSAFTFASLLSGVANVGSIR 494

Query: 378 LGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNSVTWNSMIAAFA 437
            G+Q+HS VVK    CN  V N LI+MYSKCG++D + +VF+ M  RN ++W SMI  FA
Sbjct: 495 KGEQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASRVFNFMENRNVISWTSMITGFA 554

Query: 438 RHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRS 497
           +HG A++ L+ +  M  EG KP +VT++++L ACSH GLV +G     SM +DH + P+ 
Sbjct: 555 KHGFAIRVLETFNQMIEEGVKPNEVTYVAILSACSHVGLVSEGWRHFNSMYEDHKIKPKM 614

Query: 498 EHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSKIGKYAAEHLFS 557
           EH+AC+VD+L RAG+L++A  FI  +P Q  +LVW+  LGAC ++ ++++GK AA  +  
Sbjct: 615 EHYACMVDLLCRAGLLTDAFEFINTMPFQADVLVWRTFLGACRVHSNTELGKLAARKILE 674

Query: 558 ETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEIDKKVHSFTVGDK 617
             P+ P  Y+ L+NIY+  G W+E     RKMKE    KE G SWIE+  K+H F VGD 
Sbjct: 675 LDPNEPAAYIQLSNIYACAGKWEESTEMRRKMKERNLVKEGGCSWIEVGDKIHKFYVGDT 734

Query: 618 MHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGQATHQNAIETEVVW 674
            HP    IY  L  L   +   GYVPD   +L+ L+++  D     +  +Q++ +  V +
Sbjct: 735 AHPNAHQIYDELDRLITEIKRCGYVPDTDLVLHKLEEE-NDEAEKERLLYQHSEKIAVAF 792


HSP 2 Score: 172.6 bits (436), Expect = 1.5e-41
Identity = 139/548 (25.36%), Postives = 260/548 (47.45%), Query Frame = 1

Query: 49  SFLLSICGREGNLHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFD 108
           S LL  C R  +  LG  +HA +I+ F++       V+ NSLIS+Y + G    A  VF+
Sbjct: 66  SSLLKSCIRARDFRLGKLVHARLIE-FDIEPDS---VLYNSLISLYSKSGDSAKAEDVFE 125

Query: 109 EMIT---RDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLE 168
            M     RD +SW+A++  +  NG+       F     +G    D    T ++ AC   +
Sbjct: 126 TMRRFGKRDVVSWSAMMACYGNNGRELDAIKVFVEFLELGLVPNDYC-YTAVIRACSNSD 185

Query: 169 FCWIIKMMHGLAFLSGYGQ-EITVGNALISSYFKC-GCVGLGMQVFYEMGERNVITWTAV 228
           F  + ++  G    +G+ + ++ VG +LI  + K         +VF +M E NV+TWT +
Sbjct: 186 FVGVGRVTLGFLMKTGHFESDVCVGCSLIDMFVKGENSFENAYKVFDKMSELNVVTWTLM 245

Query: 229 ISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLG 288
           I+   Q G+   +++ F +M+  G  E +  T  S+ +AC+ LE L  G Q+H   ++ G
Sbjct: 246 ITRCMQMGFPREAIRFFLDMVLSG-FESDKFTLSSVFSACAELENLSLGKQLHSWAIRSG 305

Query: 289 IQSDLCIGSALMDMYSK---SGRIGEAWKIFELAEELDMVSLTVILAGFTHN-GCEEEAI 348
           +  D  +  +L+DMY+K    G + +  K+F+  E+  ++S T ++ G+  N     EAI
Sbjct: 306 LVDD--VECSLVDMYAKCSADGSVDDCRKVFDRMEDHSVMSWTALITGYMKNCNLATEAI 365

Query: 349 QIFLKMLKMG-IEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINM 408
            +F +M+  G +E +    S      G  +  R+G+QV     K+    N  V+N +I+M
Sbjct: 366 NLFSEMITQGHVEPNHFTFSSAFKACGNLSDPRVGKQVLGQAFKRGLASNSSVANSVISM 425

Query: 409 YSKCGALDESMKVFDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTF 468
           + K   ++++ + F+ + E+N V++N+ +    R+ +  +A +L  ++       +  TF
Sbjct: 426 FVKSDRMEDAQRAFESLSEKNLVSYNTFLDGTCRNLNFEQAFKLLSEITERELGVSAFTF 485

Query: 469 LSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLP 528
            SLL   ++ G ++KG E + S     G++        ++ M  + G +  A   +    
Sbjct: 486 ASLLSGVANVGSIRKG-EQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASR-VFNFM 545

Query: 529 EQPGLLVWQALLGACSLYGDS-KIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERA 586
           E   ++ W +++   + +G + ++ +   + +      + V YV + +  S  G   E  
Sbjct: 546 ENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNEVTYVAILSACSHVGLVSEGW 603


HSP 3 Score: 167.9 bits (424), Expect = 3.7e-40
Identity = 134/519 (25.82%), Postives = 245/519 (47.21%), Query Frame = 1

Query: 150 DKATLTTILSACDGLEFCWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVF 209
           D  T +++L +C       + K++H          +  + N+LIS Y K G       VF
Sbjct: 61  DSVTFSSLLKSCIRARDFRLGKLVHARLIEFDIEPDSVLYNSLISLYSKSGDSAKAEDVF 120

Query: 210 YEM---GERNVITWTAVISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGL 269
             M   G+R+V++W+A+++    NG    ++K+F E +  G V PN   Y +++ ACS  
Sbjct: 121 ETMRRFGKRDVVSWSAMMACYGNNGRELDAIKVFVEFLELGLV-PNDYCYTAVIRACSNS 180

Query: 270 EALKEGCQIHGLIMKLG-IQSDLCIGSALMDMYSKSGRIGE-AWKIFELAEELDMVSLTV 329
           + +  G    G +MK G  +SD+C+G +L+DM+ K     E A+K+F+   EL++V+ T+
Sbjct: 181 DFVGVGRVTLGFLMKTGHFESDVCVGCSLIDMFVKGENSFENAYKVFDKMSELNVVTWTL 240

Query: 330 ILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKN 389
           ++      G   EAI+ FL M+  G E D   +S V        +L LG+Q+HS+ ++  
Sbjct: 241 MITRCMQMGFPREAIRFFLDMVLSGFESDKFTLSSVFSACAELENLSLGKQLHSWAIRSG 300

Query: 390 FICNPFVSNGLINMYSKC---GALDESMKVFDRMRERNSVTWNSMIAAFARHGD-ALKAL 449
            + +  V   L++MY+KC   G++D+  KVFDRM + + ++W ++I  + ++ + A +A+
Sbjct: 301 LVDD--VECSLVDMYAKCSADGSVDDCRKVFDRMEDHSVMSWTALITGYMKNCNLATEAI 360

Query: 450 QLYEDMQLEG-AKPTDVTFLSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVD 509
            L+ +M  +G  +P   TF S   AC +    + G + L    K  G+   S     V+ 
Sbjct: 361 NLFSEMITQGHVEPNHFTFSSAFKACGNLSDPRVGKQVLGQAFK-RGLASNSSVANSVIS 420

Query: 510 MLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSKIGKYAAEHLFSETPDSPVP 569
           M  ++  + +A+   E L E+  +     L G C      +  K  +E    E   S   
Sbjct: 421 MFVKSDRMEDAQRAFESLSEKNLVSYNTFLDGTCRNLNFEQAFKLLSEITERELGVSAFT 480

Query: 570 YVLLANIYSSEGNWKERARTIRKMKEVGTAKETGI-----------SWIEIDKKVHSFTV 629
           +  L +  ++ G+ ++  +   ++ ++G +    +             I+   +V +F  
Sbjct: 481 FASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASRVFNFME 540

Query: 630 GDKMHPQTEMIYGV--------LWELFILMVDEGYVPDK 640
              +   T MI G         + E F  M++EG  P++
Sbjct: 541 NRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNE 575


HSP 4 Score: 105.1 bits (261), Expect = 3.0e-21
Identity = 77/301 (25.58%), Postives = 139/301 (46.18%), Query Frame = 1

Query: 10  RSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGNLHLGSSLHA 69
           ++ +L +   +L S    Q H  P          NH   S     CG   +  +G  +  
Sbjct: 348 KNCNLATEAINLFSEMITQGHVEP----------NHFTFSSAFKACGNLSDPRVGKQVLG 407

Query: 70  SIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALIGGFVRNG 129
              K    SN      + NS+ISM+ +  ++ DA + F+ +  ++ +S+N  + G  RN 
Sbjct: 408 QAFKRGLASNSS----VANSVISMFVKSDRMEDAQRAFESLSEKNLVSYNTFLDGTCRNL 467

Query: 130 KFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLSGYGQEITVG 189
            F   F     +    +      T  ++LS    +      + +H      G      V 
Sbjct: 468 NFEQAFKLLSEIT-ERELGVSAFTFASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVC 527

Query: 190 NALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLFKEMMSYGSV 249
           NALIS Y KCG +    +VF  M  RNVI+WT++I+G A++G+    L+ F +M+  G V
Sbjct: 528 NALISMYSKCGSIDTASRVFNFMENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEG-V 587

Query: 250 EPNSLTYLSLLTACSGLEALKEGCQ-IHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAW 309
           +PN +TY+++L+ACS +  + EG +  + +     I+  +   + ++D+  ++G + +A+
Sbjct: 588 KPNEVTYVAILSACSHVGLVSEGWRHFNSMYEDHKIKPKMEHYACMVDLLCRAGLLTDAF 632

BLAST of Csa2G022820 vs. Swiss-Prot
Match: PP285_ARATH (Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H81 PE=2 SV=2)

HSP 1 Score: 378.6 bits (971), Expect = 1.4e-103
Identity = 208/605 (34.38%), Postives = 334/605 (55.21%), Query Frame = 1

Query: 61  LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA 120
           L +G  +HA  ++  EL++      I+N+L++MY + GKL  +  +      RD ++WN 
Sbjct: 218 LMMGKQVHAYGLRKGELNSF-----IINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNT 277

Query: 121 LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS 180
           ++    +N +      Y + M L G    D+ T++++L AC  LE     K +H  A  +
Sbjct: 278 VLSSLCQNEQLLEALEYLREMVLEG-VEPDEFTISSVLPACSHLEMLRTGKELHAYALKN 337

Query: 181 GYGQEIT-VGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKL 240
           G   E + VG+AL+  Y  C  V  G +VF  M +R +  W A+I+G +QN + + +L L
Sbjct: 338 GSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLL 397

Query: 241 FKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYS 300
           F  M     +  NS T   ++ AC    A      IHG ++K G+  D  + + LMDMYS
Sbjct: 398 FIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYS 457

Query: 301 KSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKML-----------KM 360
           + G+I  A +IF   E+ D+V+   ++ G+  +   E+A+ +  KM            ++
Sbjct: 458 RLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRV 517

Query: 361 GIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDES 420
            ++ +   +  +L    A ++L  G+++H++ +K N   +  V + L++MY+KCG L  S
Sbjct: 518 SLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMS 577

Query: 421 MKVFDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHA 480
            KVFD++ ++N +TWN +I A+  HG+  +A+ L   M ++G KP +VTF+S+  ACSH+
Sbjct: 578 RKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHS 637

Query: 481 GLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLL-VWQ 540
           G+V +G+     M  D+G+ P S+H+ACVVD+LGRAG + EA   +  +P        W 
Sbjct: 638 GMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWS 697

Query: 541 ALLGACSLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVG 600
           +LLGA  ++ + +IG+ AA++L    P+    YVLLANIYSS G W +     R MKE G
Sbjct: 698 SLLGASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAGLWDKATEVRRNMKEQG 757

Query: 601 TAKETGISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLD 653
             KE G SWIE   +VH F  GD  HPQ+E + G L  L+  M  EGYVPD   +L+ ++
Sbjct: 758 VRKEPGCSWIEHGDEVHKFVAGDSSHPQSEKLSGYLETLWERMRKEGYVPDTSCVLHNVE 816


HSP 2 Score: 210.3 bits (534), Expect = 6.5e-53
Identity = 147/554 (26.53%), Postives = 269/554 (48.56%), Query Frame = 1

Query: 12  SHLPSWVTSLISPFRNQFHQNPFPETSSTFVL---------NHLDPSFLLSICGREGNLH 71
           S  P W   L+   R++   N   E   T+V          N+  P+ L ++   + ++ 
Sbjct: 59  SRSPEWWIDLL---RSKVRSNLLREAVLTYVDMIVLGIKPDNYAFPALLKAVADLQ-DME 118

Query: 72  LGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALI 131
           LG  +HA + K        + V + N+L+++Y +CG      KVFD +  R+ +SWN+LI
Sbjct: 119 LGKQIHAHVYK---FGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLI 178

Query: 132 GGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGL---EFCWIIKMMHGLAFL 191
                  K+      F+ M L  +      TL ++++AC  L   E   + K +H     
Sbjct: 179 SSLCSFEKWEMALEAFRCM-LDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLR 238

Query: 192 SGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKL 251
            G      + N L++ Y K G +     +    G R+++TW  V+S L QN     +L+ 
Sbjct: 239 KGELNSFII-NTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEY 298

Query: 252 FKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLG-IQSDLCIGSALMDMY 311
            +EM+  G VEP+  T  S+L ACS LE L+ G ++H   +K G +  +  +GSAL+DMY
Sbjct: 299 LREMVLEG-VEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMY 358

Query: 312 SKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKM-GIEIDGNVV 371
               ++    ++F+   +  +     ++AG++ N  ++EA+ +F+ M +  G+  +   +
Sbjct: 359 CNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTM 418

Query: 372 SVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRE 431
           + V+       +    + +H FVVK+    + FV N L++MYS+ G +D +M++F +M +
Sbjct: 419 AGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMED 478

Query: 432 RNSVTWNSMIAAFA---RHGDALKALQLYEDMQLE--------GAKPTDVTFLSLLHACS 491
           R+ VTWN+MI  +     H DAL  L   ++++ +          KP  +T +++L +C+
Sbjct: 479 RDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCA 538

Query: 492 HAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVW 541
               + KG E      K++     +   A +VDM  + G L  +R   +++P Q  ++ W
Sbjct: 539 ALSALAKGKEIHAYAIKNNLATDVAVGSA-LVDMYAKCGCLQMSRKVFDQIP-QKNVITW 598

BLAST of Csa2G022820 vs. TrEMBL
Match: A0A0A0LGC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G022820 PE=4 SV=1)

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 674/674 (100.00%), Postives = 674/674 (100.00%), Query Frame = 1

Query: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN 60
           MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN
Sbjct: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN 60

Query: 61  LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA 120
           LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA
Sbjct: 61  LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA 120

Query: 121 LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS 180
           LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS
Sbjct: 121 LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS 180

Query: 181 GYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLF 240
           GYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLF
Sbjct: 181 GYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLF 240

Query: 241 KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK 300
           KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK
Sbjct: 241 KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK 300

Query: 301 SGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVV 360
           SGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVV
Sbjct: 301 SGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVV 360

Query: 361 LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS 420
           LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS
Sbjct: 361 LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS 420

Query: 421 VTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS 480
           VTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS
Sbjct: 421 VTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS 480

Query: 481 MTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK 540
           MTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK
Sbjct: 481 MTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK 540

Query: 541 IGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEID 600
           IGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEID
Sbjct: 541 IGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEID 600

Query: 601 KKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGQAT 660
           KKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGQAT
Sbjct: 601 KKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGQAT 660

Query: 661 HQNAIETEVVWELF 675
           HQNAIETEVVWELF
Sbjct: 661 HQNAIETEVVWELF 674

BLAST of Csa2G022820 vs. TrEMBL
Match: B9I7R7_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s02200g PE=4 SV=2)

HSP 1 Score: 852.0 bits (2200), Expect = 4.8e-244
Identity = 427/653 (65.39%), Postives = 517/653 (79.17%), Query Frame = 1

Query: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN 60
           MK KWV  K +SH+PSW TSL SP + + +  P  +TSS F+LNH+D   LLSICGREG 
Sbjct: 1   MKSKWVIHKLNSHIPSWATSLTSPLKAKTYHTPSSKTSS-FLLNHVDIGHLLSICGREGY 60

Query: 61  LHLGSSLHASIIKSFELSN--HYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISW 120
           LHLGSSLHASIIK+ E  N    N  VI NSL+SMY + G L DA K+FDEM  RDT+SW
Sbjct: 61  LHLGSSLHASIIKTHEFFNPLEQNAFVIWNSLLSMYAKNGVLTDAAKLFDEMPMRDTVSW 120

Query: 121 NALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAF 180
           N +I GF+++G F  GF +FK M  +G  R D+ATLTTILSACD  E  ++ KM+H LA 
Sbjct: 121 NIMISGFLKDGSFDVGFGFFKQMQSLGFYRLDQATLTTILSACDRPELGFVNKMVHCLAV 180

Query: 181 LSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLK 240
           L+G+ +EI+VGNALI+SYFKCG    GMQVF EM ERNVITWTA+ISGL Q+  +  SL+
Sbjct: 181 LNGFQREISVGNALITSYFKCGFSSSGMQVFDEMLERNVITWTAIISGLVQSELYRDSLR 240

Query: 241 LFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMY 300
           LF EM + G VEPNSLTYLS L ACSGL+AL+EGCQIHG + KLG+QSD C+ SALMDMY
Sbjct: 241 LFVEMTN-GLVEPNSLTYLSSLMACSGLQALREGCQIHGRLWKLGLQSDFCVESALMDMY 300

Query: 301 SKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVS 360
           SK G +G+  +IFE A +LD VS+T+ILAGF  NG EEEA+Q F+KML+ G EID N+VS
Sbjct: 301 SKCGSMGDTLQIFESAGQLDKVSMTIILAGFAQNGFEEEAMQFFVKMLEAGTEIDSNMVS 360

Query: 361 VVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRER 420
            VLGVFGADTSL LGQQ+HS V+K++F  NPFV NGLINMYSKCG L++S KVF RM   
Sbjct: 361 AVLGVFGADTSLGLGQQIHSLVIKRSFGSNPFVGNGLINMYSKCGDLEDSTKVFSRMPCM 420

Query: 421 NSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFL 480
           NSV+WNSMIAAFARHGD  +ALQLY++M+L+G +PTDVTFLSLLHACSH GLV+KGMEFL
Sbjct: 421 NSVSWNSMIAAFARHGDGSRALQLYKEMRLKGVEPTDVTFLSLLHACSHVGLVEKGMEFL 480

Query: 481 KSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGD 540
           KSMT+ H + PR EH+ACVVDMLGRAG+L+EA+ FIE LP +P +LVWQALLGAC ++GD
Sbjct: 481 KSMTEVHKLTPRMEHYACVVDMLGRAGLLNEAKTFIEGLPIKPDVLVWQALLGACGIHGD 540

Query: 541 SKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIE 600
            ++GKYAAEHL    P+ P PY+LLANIYSS+G WKERA+TI++MKE+  AKETGISWIE
Sbjct: 541 PEMGKYAAEHLILSAPEKPSPYILLANIYSSKGRWKERAKTIKRMKEMCVAKETGISWIE 600

Query: 601 IDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRR 652
           I+  +HSF V DKMHPQ E+IYGVL ELF  M+DEGYVPDK++IL Y++ D +
Sbjct: 601 IENNLHSFVVEDKMHPQAEIIYGVLAELFGHMIDEGYVPDKRYILSYVNQDEK 651

BLAST of Csa2G022820 vs. TrEMBL
Match: M5WNH2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023606mg PE=4 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 2.0e-242
Identity = 427/647 (66.00%), Postives = 514/647 (79.44%), Query Frame = 1

Query: 1   MKLKWVFQKR---SSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGR 60
           MK +WVFQ +   S +L SW +SLISPF+ +  QNP  ET+S  +LNH+D S LLS+CG+
Sbjct: 1   MKSRWVFQFQNLNSHYLSSWASSLISPFKAKVQQNPSAETTSRLILNHVDISLLLSLCGK 60

Query: 61  EGNLHLGSSLHASIIKSFEL-----SNHYNGV-VIMNSLISMYDRCGKLPDAVKVFDEMI 120
           EGN HLGSSLHASIIK+ E       + Y  V V+ NSL+S+Y +CG+  +AVK+FD M 
Sbjct: 61  EGNFHLGSSLHASIIKNPEFFYPESQDDYRYVLVVWNSLLSVYLKCGQFSNAVKLFDNMG 120

Query: 121 TRDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIK 180
            +DT+SWN +I GF RNG+   GF YFK M      RFD+ATLT+IL+A DG EFC + K
Sbjct: 121 MKDTVSWNTMISGFFRNGESDVGFGYFKQMRGSDFYRFDRATLTSILAAFDGPEFCHLNK 180

Query: 181 MMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNG 240
           MMHGL  L+G+ +E  VGNALI+SY KCG  G G +VF EM ERNVITWTA+ISGLAQN 
Sbjct: 181 MMHGLVVLNGFERETAVGNALITSYCKCGSFGSGRRVFDEMFERNVITWTAMISGLAQNE 240

Query: 241 YHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIG 300
           ++  SL+LF EM S G V+PNSLTYL+ LTACSGL+A+  G QIHGL  KLGIQSDLCI 
Sbjct: 241 FYVESLELFLEMRS-GVVDPNSLTYLASLTACSGLQAISVGRQIHGLAWKLGIQSDLCIE 300

Query: 301 SALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIE 360
           SALMDMYSK G + +AW+IFE  EELD +S+TVIL GF  NG E EAIQIF+KM+K GIE
Sbjct: 301 SALMDMYSKCGSLEDAWRIFESTEELDEISMTVILVGFAQNGFESEAIQIFVKMMKAGIE 360

Query: 361 IDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKV 420
           ID N+VS VLGVFG DTSL LG+Q+HS +VKK+F  N FV NGLINMYSKCG L +S+KV
Sbjct: 361 IDPNMVSAVLGVFGVDTSLGLGKQLHSLIVKKSFGHNSFVCNGLINMYSKCGELGDSVKV 420

Query: 421 FDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLV 480
           F RM +RNS++WNSMIAAFARHGD  KALQLYE+M+++G +PTDVTFLSLLHACSH G V
Sbjct: 421 FSRMPQRNSISWNSMIAAFARHGDGSKALQLYEEMKMDGVQPTDVTFLSLLHACSHVGFV 480

Query: 481 KKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLG 540
           ++GMEFL SM +D G++PR EH+ACVVDMLGRAG+L++A+NFIE LPE PG+LVWQALLG
Sbjct: 481 ERGMEFLNSMNEDPGISPRPEHYACVVDMLGRAGLLTDAKNFIEGLPENPGVLVWQALLG 540

Query: 541 ACSLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKE 600
           ACS++GDS+IGKYAA+ L    P++P PYVLLANIYSSEG WKERARTI+ MKE+G AKE
Sbjct: 541 ACSIHGDSEIGKYAADQLLLAAPETPAPYVLLANIYSSEGRWKERARTIKGMKELGVAKE 600

Query: 601 TGISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPD 639
           TGISWIEI+ KV SF VGD+MHPQ E+IYGVL EL+ LM DEGYVP+
Sbjct: 601 TGISWIEIENKVQSFVVGDRMHPQAEIIYGVLAELYRLMTDEGYVPN 646

BLAST of Csa2G022820 vs. TrEMBL
Match: A0A0R0KNE9_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_03G238000 PE=4 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 1.3e-241
Identity = 420/628 (66.88%), Postives = 510/628 (81.21%), Query Frame = 1

Query: 31  QNPFPETSSTFVLNHLDPSFLLSICGREGNLHLGSSLHASIIK---SFEL-SNHYNGVVI 90
           QNPFP TS + VLNH D S LLS+CGR+GNL+LGSS+HA IIK   SF+  S+  + + +
Sbjct: 8   QNPFPATSKS-VLNHADLSSLLSVCGRDGNLNLGSSIHARIIKQPPSFDFDSSPRDALFV 67

Query: 91  MNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGD 150
            NSL+SMY +CGKL DA+K+FD M  +DT+SWNA+I GF+RN     GF +F+ M     
Sbjct: 68  WNSLLSMYSKCGKLQDAIKLFDHMPVKDTVSWNAIISGFLRNRDCDTGFRFFRQMSESRT 127

Query: 151 --CRFDKATLTTILSACDGLEFCWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGL 210
             C FDKATLTT+LSACDGLEF  + KM+H L F+ G+ +EITVGNALI+SYFKCGC   
Sbjct: 128 VCCLFDKATLTTMLSACDGLEFSSVTKMIHCLVFVGGFEREITVGNALITSYFKCGCFSQ 187

Query: 211 GMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACS 270
           G QVF EM ERNV+TWTAVISGLAQN ++E  L+LF +M   GSV PNSLTYLS L ACS
Sbjct: 188 GRQVFDEMLERNVVTWTAVISGLAQNEFYEDGLRLFDQMRR-GSVSPNSLTYLSALMACS 247

Query: 271 GLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIFELAEELDMVSLTV 330
           GL+AL EG +IHGL+ KLG+QSDLCI SALMD+YSK G + EAW+IFE AEELD VSLTV
Sbjct: 248 GLQALLEGRKIHGLLWKLGMQSDLCIESALMDLYSKCGSLEEAWEIFESAEELDDVSLTV 307

Query: 331 ILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKN 390
           IL  F  NG EEEAIQIF++M+K+GIE+D N+VS +LGVFG  TSL LG+Q+HS ++KKN
Sbjct: 308 ILVAFMQNGLEEEAIQIFMRMVKLGIEVDPNMVSAILGVFGVGTSLTLGKQIHSLIIKKN 367

Query: 391 FICNPFVSNGLINMYSKCGALDESMKVFDRMRERNSVTWNSMIAAFARHGDALKALQLYE 450
           FI N FVSNGLINMYSKCG L +S++VF  M ++NSV+WNS+IAA+AR+GD  +ALQ Y+
Sbjct: 368 FIQNLFVSNGLINMYSKCGDLYDSLQVFHEMTQKNSVSWNSVIAAYARYGDGFRALQFYD 427

Query: 451 DMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRA 510
           DM++EG   TDVTFLSLLHACSHAGLV+KGMEFL+SMT+DHG++PRSEH+ACVVDMLGRA
Sbjct: 428 DMRVEGIALTDVTFLSLLHACSHAGLVEKGMEFLESMTRDHGLSPRSEHYACVVDMLGRA 487

Query: 511 GMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSKIGKYAAEHLFSETPDSPVPYVLLA 570
           G+L EA+ FIE LPE PG+LVWQALLGACS++GDS++GKYAA  LF  TPDSP PYVL+A
Sbjct: 488 GLLKEAKKFIEGLPENPGVLVWQALLGACSIHGDSEMGKYAANQLFLATPDSPAPYVLMA 547

Query: 571 NIYSSEGNWKERARTIRKMKEVGTAKETGISWIEIDKKVHSFTVGDKMHPQTEMIYGVLW 630
           NIYSSEG WKERAR+I+KMKE+G AKE GISW+EI+KKV+SF VGDKMHPQ + I+ +L 
Sbjct: 548 NIYSSEGKWKERARSIKKMKEMGVAKEVGISWVEIEKKVNSFVVGDKMHPQADAIFWLLS 607

Query: 631 ELFILMVDEGYVPDKKFILYYLDDDRRD 653
            L   + DEGYVPDK+ ILYYLD D++D
Sbjct: 608 RLLKHLKDEGYVPDKRCILYYLDQDKKD 633

BLAST of Csa2G022820 vs. TrEMBL
Match: A0A0L9VDX2_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan09g152300 PE=4 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 2.7e-239
Identity = 421/664 (63.40%), Postives = 518/664 (78.01%), Query Frame = 1

Query: 1   MKLKWVFQKRSSHLPSWVTSLISPF--RNQFHQNPFPETSSTFVLNHLDPSFLLSICGRE 60
           MK +W F   S+ LPSW+ SL  PF  +    QNPF  TS + VLNH + S LLS+CGR+
Sbjct: 1   MKSRWNF---SAQLPSWLDSLNIPFTMKAPTFQNPFLTTSKS-VLNHTELSSLLSVCGRD 60

Query: 61  GNLHLGSSLHASIIKSFEL----SNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRD 120
           GNL+LGSS+HA IIK        S+  N + + NSL+S+Y +CG+L DAVK+FD M  +D
Sbjct: 61  GNLNLGSSIHARIIKQPPFYDSDSSLRNALFVWNSLLSVYTKCGQLQDAVKLFDHMPVKD 120

Query: 121 TISWNALIGGFVRNGKFFAGFSYFK------AMCLVGDCRFDKATLTTILSACDGLEFCW 180
           T+SWN +I GF+RN     GF  FK       +C    CRFDKATL+T+LSACDGLEF  
Sbjct: 121 TVSWNTMISGFLRNRDLDTGFRLFKQTSESRTVC----CRFDKATLSTMLSACDGLEFSG 180

Query: 181 IIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLA 240
             KM+HGL F+ G+ +E+TVGNALI+SYFKCGC   G QVFYEM ERNVITWTAVISGLA
Sbjct: 181 ATKMVHGLVFVGGFEREVTVGNALITSYFKCGCFSEGKQVFYEMLERNVITWTAVISGLA 240

Query: 241 QNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDL 300
           ++ ++E  L+LF +M  +G V PNSLTYLS L ACSGL+AL +G +IHG++ KLG+QSDL
Sbjct: 241 KSEFYEDGLRLFNQMR-WGPVSPNSLTYLSALMACSGLQALSDGRKIHGMLWKLGMQSDL 300

Query: 301 CIGSALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKM 360
           CI SALMD+YSK G +  AW+IFE AEELD VSLTVIL  FT NG EEEAIQIF++M+K+
Sbjct: 301 CIESALMDLYSKCGSLEAAWQIFEFAEELDEVSLTVILVAFTQNGLEEEAIQIFMRMVKL 360

Query: 361 GIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDES 420
           GIE+D N+VS +LGVF   TSL  G+Q+HS ++KKNF  N FVSNGLINMYSKCG L +S
Sbjct: 361 GIEVDPNMVSAILGVFVLGTSLTFGKQIHSLIIKKNFSQNLFVSNGLINMYSKCGDLYDS 420

Query: 421 MKVFDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHA 480
           ++VF  M ++NSV+WNS+IAA+AR+GD  +ALQ Y+ M+++G  PTDVTFLSLLHACSHA
Sbjct: 421 LQVFREMTQKNSVSWNSVIAAYARYGDGFRALQFYDAMRVDGIAPTDVTFLSLLHACSHA 480

Query: 481 GLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQA 540
           GLV+KGMEFL+SMTKDHG++PRSEH+ACVVDMLGRAG+L EA+ FIE LPE PG+LVWQA
Sbjct: 481 GLVEKGMEFLESMTKDHGLSPRSEHYACVVDMLGRAGLLKEAKRFIEGLPENPGVLVWQA 540

Query: 541 LLGACSLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGT 600
           LLGAC ++GDS++GKYAA+ LF  TPDSP PYVL+ANIYSSEG WKERAR I++MKE+G 
Sbjct: 541 LLGACGIHGDSEMGKYAADKLFLATPDSPAPYVLMANIYSSEGKWKERARAIKRMKEMGV 600

Query: 601 AKETGISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDD 653
           AKE GISWIEI+KKV+SF  GDKMHPQ + I+ +L  L   + DEGYVPDK+ ILYYLD 
Sbjct: 601 AKEVGISWIEIEKKVNSFVAGDKMHPQADDIFCLLSVLLEHLKDEGYVPDKRCILYYLDQ 655

BLAST of Csa2G022820 vs. TAIR10
Match: AT3G05340.1 (AT3G05340.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 748.4 bits (1931), Expect = 3.8e-216
Identity = 388/661 (58.70%), Postives = 473/661 (71.56%), Query Frame = 1

Query: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN 60
           M  +WV QK +SHLPS +++++SP +    Q+P  + S TF+LNH+D S LLSICGREG 
Sbjct: 1   MNSRWVIQKLTSHLPSCLSTVLSPSKILIRQSPNYQVS-TFLLNHVDMSLLLSICGREGW 60

Query: 61  L-HLGSSLHASIIKSFELSN------HYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITR 120
             HLG  LHASIIK+ E         H N +V+ NSL+S+Y +CGKL DA+K+FDEM  R
Sbjct: 61  FPHLGPCLHASIIKNPEFFEPVDADIHRNALVVWNSLLSLYAKCGKLVDAIKLFDEMPMR 120

Query: 121 DTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMM 180
           D IS N +  GF+RN +  +GF   K M  +G   FD ATLT +LS CD  EFC + KM+
Sbjct: 121 DVISQNIVFYGFLRNRETESGFVLLKRM--LGSGGFDHATLTIVLSVCDTPEFCLVTKMI 180

Query: 181 HGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYH 240
           H LA LSGY +EI+VGN LI+SYFKCGC   G  VF  M  RNVIT TAVISGL +N  H
Sbjct: 181 HALAILSGYDKEISVGNKLITSYFKCGCSVSGRGVFDGMSHRNVITLTAVISGLIENELH 240

Query: 241 EHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSA 300
           E  L+LF  +M  G V PNS+TYLS L ACSG + + EG QIH L+ K GI+S+LCI SA
Sbjct: 241 EDGLRLFS-LMRRGLVHPNSVTYLSALAACSGSQRIVEGQQIHALLWKYGIESELCIESA 300

Query: 301 LMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEID 360
           LMDMYSK G I +AW IFE   E+D VS+TVIL G   NG EEEAIQ F++ML+ G+EID
Sbjct: 301 LMDMYSKCGSIEDAWTIFESTTEVDEVSMTVILVGLAQNGSEEEAIQFFIRMLQAGVEID 360

Query: 361 GNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFD 420
            NVVS VLGV   D SL LG+Q+HS V+K+ F  N FV+NGLINMYSKCG L +S  VF 
Sbjct: 361 ANVVSAVLGVSFIDNSLGLGKQLHSLVIKRKFSGNTFVNNGLINMYSKCGDLTDSQTVFR 420

Query: 421 RMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKK 480
           RM +RN V+WNSMIAAFARHG  L AL+LYE+M     KPTDVTFLSLLHACSH GL+ K
Sbjct: 421 RMPKRNYVSWNSMIAAFARHGHGLAALKLYEEMTTLEVKPTDVTFLSLLHACSHVGLIDK 480

Query: 481 GMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGAC 540
           G E L  M + HG+ PR+EH+ C++DMLGRAG+L EA++FI+ LP +P   +WQALLGAC
Sbjct: 481 GRELLNEMKEVHGIEPRTEHYTCIIDMLGRAGLLKEAKSFIDSLPLKPDCKIWQALLGAC 540

Query: 541 SLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETG 600
           S +GD+++G+YAAE LF   PDS   ++L+ANIYSS G WKERA+TI++MK +G  KETG
Sbjct: 541 SFHGDTEVGEYAAEQLFQTAPDSSSAHILIANIYSSRGKWKERAKTIKRMKAMGVTKETG 600

Query: 601 ISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDP 655
           IS IEI+ K HSF V DK+HPQ E IY VL  LF +MVDEGY PDK+FIL Y  DDR   
Sbjct: 601 ISSIEIEHKTHSFVVEDKLHPQAEAIYDVLSGLFPVMVDEGYRPDKRFILCYTGDDRNGT 657

BLAST of Csa2G022820 vs. TAIR10
Match: AT5G09950.1 (AT5G09950.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 386.7 bits (992), Expect = 2.9e-107
Identity = 217/579 (37.48%), Postives = 336/579 (58.03%), Query Frame = 1

Query: 84  VVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALIGGFVRNGKFFAGFSYFKAMCL 143
           V I N L++MY +CG + DA +VF  M  +D++SWN++I G  +NG F      +K+M  
Sbjct: 349 VGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVERYKSMRR 408

Query: 144 VGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVG 203
             D      TL + LS+C  L++  + + +HG +   G    ++V NAL++ Y + G + 
Sbjct: 409 -HDILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVSVSNALMTLYAETGYLN 468

Query: 204 LGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLK----LFKEMMSYGSVEPNSLTYLSL 263
              ++F  M E + ++W ++I  LA++   E SL      F      G  + N +T+ S+
Sbjct: 469 ECRKIFSSMPEHDQVSWNSIIGALARS---ERSLPEAVVCFLNAQRAGQ-KLNRITFSSV 528

Query: 264 LTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIFE-LAEELD 323
           L+A S L   + G QIHGL +K  I  +    +AL+  Y K G +    KIF  +AE  D
Sbjct: 529 LSAVSSLSFGELGKQIHGLALKNNIADEATTENALIACYGKCGEMDGCEKIFSRMAERRD 588

Query: 324 MVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVFGADTSLRLGQQVHS 383
            V+   +++G+ HN    +A+ +   ML+ G  +D  + + VL  F +  +L  G +VH+
Sbjct: 589 NVTWNSMISGYIHNELLAKALDLVWFMLQTGQRLDSFMYATVLSAFASVATLERGMEVHA 648

Query: 384 FVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNSVTWNSMIAAFARHGDALK 443
             V+     +  V + L++MYSKCG LD +++ F+ M  RNS +WNSMI+ +ARHG   +
Sbjct: 649 CSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVRNSYSWNSMISGYARHGQGEE 708

Query: 444 ALQLYEDMQLEGAKPTD-VTFLSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSEHHACV 503
           AL+L+E M+L+G  P D VTF+ +L ACSHAGL+++G +  +SM+  +G+ PR EH +C+
Sbjct: 709 ALKLFETMKLDGQTPPDHVTFVGVLSACSHAGLLEEGFKHFESMSDSYGLAPRIEHFSCM 768

Query: 504 VDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK--IGKYAAEHLFSETPD 563
            D+LGRAG L +  +FIEK+P +P +L+W+ +LGAC      K  +GK AAE LF   P+
Sbjct: 769 ADVLGRAGELDKLEDFIEKMPMKPNVLIWRTVLGACCRANGRKAELGKKAAEMLFQLEPE 828

Query: 564 SPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEIDKKVHSFTVGDKMHPQ 623
           + V YVLL N+Y++ G W++  +  +KMK+    KE G SW+ +   VH F  GDK HP 
Sbjct: 829 NAVNYVLLGNMYAAGGRWEDLVKARKKMKDADVKKEAGYSWVTMKDGVHMFVAGDKSHPD 888

Query: 624 TEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPI 655
            ++IY  L EL   M D GYVP   F LY L+ + ++ I
Sbjct: 889 ADVIYKKLKELNRKMRDAGYVPQTGFALYDLEQENKEEI 922


HSP 2 Score: 205.7 bits (522), Expect = 9.1e-53
Identity = 137/460 (29.78%), Postives = 232/460 (50.43%), Query Frame = 1

Query: 84  VVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALIGGFVRNGKFFAGFSYFKAMCL 143
           V + N+LI+ Y   G    A KVFDEM  R+ +SW  ++ G+ RNG+      + + M  
Sbjct: 36  VYLCNNLINAYLETGDSVSARKVFDEMPLRNCVSWACIVSGYSRNGEHKEALVFLRDMVK 95

Query: 144 VGDCRFDKATLTTILSACDGLEFCWII--KMMHGLAFLSGYGQEITVGNALISSYFKC-G 203
            G    ++    ++L AC  +    I+  + +HGL F   Y  +  V N LIS Y+KC G
Sbjct: 96  EGIFS-NQYAFVSVLRACQEIGSVGILFGRQIHGLMFKLSYAVDAVVSNVLISMYWKCIG 155

Query: 204 CVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLL 263
            VG  +  F ++  +N ++W ++IS  +Q G    + ++F  M   GS  P   T+ SL+
Sbjct: 156 SVGYALCAFGDIEVKNSVSWNSIISVYSQAGDQRSAFRIFSSMQYDGS-RPTEYTFGSLV 215

Query: 264 T-ACSGLEA-LKEGCQIHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIFELAEELD 323
           T ACS  E  ++   QI   I K G+ +DL +GS L+  ++KSG +  A K+F   E  +
Sbjct: 216 TTACSLTEPDVRLLEQIMCTIQKSGLLTDLFVGSGLVSAFAKSGSLSYARKVFNQMETRN 275

Query: 324 MVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVF-----GADTSLRLG 383
            V+L  ++ G       EEA ++F+ M  M I++      ++L  F       +  L+ G
Sbjct: 276 AVTLNGLMVGLVRQKWGEEATKLFMDMNSM-IDVSPESYVILLSSFPEYSLAEEVGLKKG 335

Query: 384 QQVHSFVVKKNFICNPF-VSNGLINMYSKCGALDESMKVFDRMRERNSVTWNSMIAAFAR 443
           ++VH  V+    +     + NGL+NMY+KCG++ ++ +VF  M +++SV+WNSMI    +
Sbjct: 336 REVHGHVITTGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQ 395

Query: 444 HGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSE 503
           +G  ++A++ Y+ M+     P   T +S L +C+     K G +      K  G++    
Sbjct: 396 NGCFIEAVERYKSMRRHDILPGSFTLISSLSSCASLKWAKLGQQIHGESLK-LGIDLNVS 455

Query: 504 HHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGA 533
               ++ +    G L+E R     +PE    + W +++GA
Sbjct: 456 VSNALMTLYAETGYLNECRKIFSSMPEH-DQVSWNSIIGA 490


HSP 3 Score: 193.7 bits (491), Expect = 3.6e-49
Identity = 147/538 (27.32%), Postives = 257/538 (47.77%), Query Frame = 1

Query: 58  EGNLHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTIS 117
           E ++ L   +  +I KS  L++ + G    + L+S + + G L  A KVF++M TR+ ++
Sbjct: 221 EPDVRLLEQIMCTIQKSGLLTDLFVG----SGLVSAFAKSGSLSYARKVFNQMETRNAVT 280

Query: 118 WNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKM----- 177
            N L+ G VR          F  M  + D   +   +  +LS+         + +     
Sbjct: 281 LNGLMVGLVRQKWGEEATKLFMDMNSMIDVSPESYVI--LLSSFPEYSLAEEVGLKKGRE 340

Query: 178 MHGLAFLSGYGQ-EITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNG 237
           +HG    +G     + +GN L++ Y KCG +    +VFY M +++ ++W ++I+GL QNG
Sbjct: 341 VHGHVITTGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQNG 400

Query: 238 YHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIG 297
               +++ +K M  +  + P S T +S L++C+ L+  K G QIHG  +KLGI  ++ + 
Sbjct: 401 CFIEAVERYKSMRRH-DILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVSVS 460

Query: 298 SALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCE-EEAIQIFLKMLKMGI 357
           +ALM +Y+++G + E  KIF    E D VS   I+     +     EA+  FL   + G 
Sbjct: 461 NALMTLYAETGYLNECRKIFSSMPEHDQVSWNSIIGALARSERSLPEAVVCFLNAQRAGQ 520

Query: 358 EIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMK 417
           +++    S VL    + +   LG+Q+H   +K N        N LI  Y KCG +D   K
Sbjct: 521 KLNRITFSSVLSAVSSLSFGELGKQIHGLALKNNIADEATTENALIACYGKCGEMDGCEK 580

Query: 418 VFDRMRE-RNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAG 477
           +F RM E R++VTWNSMI+ +  +    KAL L   M   G +     + ++L A +   
Sbjct: 581 IFSRMAERRDNVTWNSMISGYIHNELLAKALDLVWFMLQTGQRLDSFMYATVLSAFASVA 640

Query: 478 LVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQAL 537
            +++GME + + +    +       + +VDM  + G L  A  F   +P +     W ++
Sbjct: 641 TLERGME-VHACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVR-NSYSWNSM 700

Query: 538 LGACSLY--GDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKE 586
           +   + +  G+  +  +    L  +TP   V +V + +  S  G  +E  +    M +
Sbjct: 701 ISGYARHGQGEEALKLFETMKLDGQTPPDHVTFVGVLSACSHAGLLEEGFKHFESMSD 749


HSP 4 Score: 175.6 bits (444), Expect = 1.0e-43
Identity = 133/491 (27.09%), Postives = 241/491 (49.08%), Query Frame = 1

Query: 51  LLSICGREGNLHL--GSSLHASIIKSFELSNHYNGVVIMNSLISMYDRC-GKLPDAVKVF 110
           +L  C   G++ +  G  +H  +   F+LS   + VV  N LISMY +C G +  A+  F
Sbjct: 108 VLRACQEIGSVGILFGRQIHGLM---FKLSYAVDAVV-SNVLISMYWKCIGSVGYALCAF 167

Query: 111 DEMITRDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLE-F 170
            ++  ++++SWN++I  + + G   + F  F +M   G    +    + + +AC   E  
Sbjct: 168 GDIEVKNSVSWNSIISVYSQAGDQRSAFRIFSSMQYDGSRPTEYTFGSLVTTACSLTEPD 227

Query: 171 CWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISG 230
             +++ +      SG   ++ VG+ L+S++ K G +    +VF +M  RN +T   ++ G
Sbjct: 228 VRLLEQIMCTIQKSGLLTDLFVGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVG 287

Query: 231 LAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLE-----ALKEGCQIHGLIMK 290
           L +  + E + KLF +M S   V P S  Y+ LL++           LK+G ++HG ++ 
Sbjct: 288 LVRQKWGEEATKLFMDMNSMIDVSPES--YVILLSSFPEYSLAEEVGLKKGREVHGHVIT 347

Query: 291 LGIQSDLC-IGSALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQ 350
            G+   +  IG+ L++MY+K G I +A ++F    + D VS   ++ G   NGC  EA++
Sbjct: 348 TGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVE 407

Query: 351 IFLKMLKMGIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYS 410
            +  M +  I      +   L    +    +LGQQ+H   +K     N  VSN L+ +Y+
Sbjct: 408 RYKSMRRHDILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVSVSNALMTLYA 467

Query: 411 KCGALDESMKVFDRMRERNSVTWNSMIAAFARHGDAL-KALQLYEDMQLEGAKPTDVTFL 470
           + G L+E  K+F  M E + V+WNS+I A AR   +L +A+  + + Q  G K   +TF 
Sbjct: 468 ETGYLNECRKIFSSMPEHDQVSWNSIIGALARSERSLPEAVVCFLNAQRAGQKLNRITFS 527

Query: 471 SLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPE 530
           S+L A S     + G +      K++  +  +  +A ++   G+ G +        ++ E
Sbjct: 528 SVLSAVSSLSFGELGKQIHGLALKNNIADEATTENA-LIACYGKCGEMDGCEKIFSRMAE 587


HSP 5 Score: 115.9 bits (289), Expect = 9.5e-26
Identity = 92/327 (28.13%), Postives = 152/327 (46.48%), Query Frame = 1

Query: 43  LNHLDPSFLLSICGREGNLHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPD 102
           LN +  S +LS         LG  +H   +K+    N  +     N+LI+ Y +CG++  
Sbjct: 515 LNRITFSSVLSAVSSLSFGELGKQIHGLALKN----NIADEATTENALIACYGKCGEMDG 574

Query: 103 AVKVFDEMITR-DTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSAC 162
             K+F  M  R D ++WN++I G++ N            M   G  R D     T+LSA 
Sbjct: 575 CEKIFSRMAERRDNVTWNSMISGYIHNELLAKALDLVWFMLQTGQ-RLDSFMYATVLSAF 634

Query: 163 DGLEFCWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWT 222
             +        +H  +  +    ++ VG+AL+  Y KCG +   ++ F  M  RN  +W 
Sbjct: 635 ASVATLERGMEVHACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVRNSYSWN 694

Query: 223 AVISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQ-IHGLIM 282
           ++ISG A++G  E +LKLF+ M   G   P+ +T++ +L+ACS    L+EG +    +  
Sbjct: 695 SMISGYARHGQGEEALKLFETMKLDGQTPPDHVTFVGVLSACSHAGLLEEGFKHFESMSD 754

Query: 283 KLGIQSDLCIGSALMDMYSKSGRIGEAWKIFELAEELDM-----VSLTVILAGFTHNGCE 342
             G+   +   S + D+    GR GE  K+ +  E++ M     +  TV+ A    NG +
Sbjct: 755 SYGLAPRIEHFSCMADVL---GRAGELDKLEDFIEKMPMKPNVLIWRTVLGACCRANGRK 814

Query: 343 EEAIQIFLKMLKMGIEIDGNVVSVVLG 363
            E  +   +ML   +E +  V  V+LG
Sbjct: 815 AELGKKAAEML-FQLEPENAVNYVLLG 832


HSP 6 Score: 102.4 bits (254), Expect = 1.1e-21
Identity = 94/399 (23.56%), Postives = 179/399 (44.86%), Query Frame = 1

Query: 257 LSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIFELAEE 316
           LS + +C G          H  + K  +  D+ + + L++ Y ++G    A K+F+    
Sbjct: 7   LSFVQSCVGHRGAARF--FHSRLYKNRLDKDVYLCNNLINAYLETGDSVSARKVFDEMPL 66

Query: 317 LDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVFG--ADTSLRLGQ 376
            + VS   I++G++ NG  +EA+     M+K GI  +      VL          +  G+
Sbjct: 67  RNCVSWACIVSGYSRNGEHKEALVFLRDMVKEGIFSNQYAFVSVLRACQEIGSVGILFGR 126

Query: 377 QVHSFVVKKNFICNPFVSNGLINMYSKC-GALDESMKVFDRMRERNSVTWNSMIAAFARH 436
           Q+H  + K ++  +  VSN LI+MY KC G++  ++  F  +  +NSV+WNS+I+ +++ 
Sbjct: 127 QIHGLMFKLSYAVDAVVSNVLISMYWKCIGSVGYALCAFGDIEVKNSVSWNSIISVYSQA 186

Query: 437 GDALKALQLYEDMQLEGAKPTDVTFLSLL-HACSHAGLVKKGMEFLKSMTKDHGMNPRSE 496
           GD   A +++  MQ +G++PT+ TF SL+  ACS      + +E +    +  G+     
Sbjct: 187 GDQRSAFRIFSSMQYDGSRPTEYTFGSLVTTACSLTEPDVRLLEQIMCTIQKSGLLTDLF 246

Query: 497 HHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSKIGKYAAE---HL 556
             + +V    ++G LS AR    ++  +  + +   ++G        K G+ A +    +
Sbjct: 247 VGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVGLVR----QKWGEEATKLFMDM 306

Query: 557 FSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEIDKKVHSFTVG 616
            S    SP  YV+L                +    E   A+E G   ++  ++VH   + 
Sbjct: 307 NSMIDVSPESYVIL----------------LSSFPEYSLAEEVG---LKKGREVHGHVIT 366

Query: 617 DKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDD 649
             +    + + G+   L  +    G + D + + Y++ D
Sbjct: 367 TGL---VDFMVGIGNGLVNMYAKCGSIADARRVFYFMTD 377

BLAST of Csa2G022820 vs. TAIR10
Match: AT3G49170.1 (AT3G49170.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 380.9 bits (977), Expect = 1.6e-105
Identity = 215/602 (35.71%), Postives = 334/602 (55.48%), Query Frame = 1

Query: 78  SNHYNGVVIMN-SLISMYDRC-GKLPDAVKVFDEMITRDTISWNALIGGFVRNGKFFAGF 137
           + H+   V +  SLI M+ +      +A KVFD+M   + ++W  +I   ++ G      
Sbjct: 195 TGHFESDVCVGCSLIDMFVKGENSFENAYKVFDKMSELNVVTWTLMITRCMQMGFPREAI 254

Query: 138 SYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLSGYGQEITVGNALISS 197
            +F  M L G    DK TL+++ SAC  LE   + K +H  A  SG   ++    +L+  
Sbjct: 255 RFFLDMVLSG-FESDKFTLSSVFSACAELENLSLGKQLHSWAIRSGLVDDVEC--SLVDM 314

Query: 198 YFKC---GCVGLGMQVFYEMGERNVITWTAVISGLAQN-GYHEHSLKLFKEMMSYGSVEP 257
           Y KC   G V    +VF  M + +V++WTA+I+G  +N      ++ LF EM++ G VEP
Sbjct: 315 YAKCSADGSVDDCRKVFDRMEDHSVMSWTALITGYMKNCNLATEAINLFSEMITQGHVEP 374

Query: 258 NSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIF 317
           N  T+ S   AC  L   + G Q+ G   K G+ S+  + ++++ M+ KS R+ +A + F
Sbjct: 375 NHFTFSSAFKACGNLSDPRVGKQVLGQAFKRGLASNSSVANSVISMFVKSDRMEDAQRAF 434

Query: 318 ELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVFGADTSLR 377
           E   E ++VS    L G   N   E+A ++  ++ +  + +     + +L       S+R
Sbjct: 435 ESLSEKNLVSYNTFLDGTCRNLNFEQAFKLLSEITERELGVSAFTFASLLSGVANVGSIR 494

Query: 378 LGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNSVTWNSMIAAFA 437
            G+Q+HS VVK    CN  V N LI+MYSKCG++D + +VF+ M  RN ++W SMI  FA
Sbjct: 495 KGEQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASRVFNFMENRNVISWTSMITGFA 554

Query: 438 RHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRS 497
           +HG A++ L+ +  M  EG KP +VT++++L ACSH GLV +G     SM +DH + P+ 
Sbjct: 555 KHGFAIRVLETFNQMIEEGVKPNEVTYVAILSACSHVGLVSEGWRHFNSMYEDHKIKPKM 614

Query: 498 EHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSKIGKYAAEHLFS 557
           EH+AC+VD+L RAG+L++A  FI  +P Q  +LVW+  LGAC ++ ++++GK AA  +  
Sbjct: 615 EHYACMVDLLCRAGLLTDAFEFINTMPFQADVLVWRTFLGACRVHSNTELGKLAARKILE 674

Query: 558 ETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEIDKKVHSFTVGDK 617
             P+ P  Y+ L+NIY+  G W+E     RKMKE    KE G SWIE+  K+H F VGD 
Sbjct: 675 LDPNEPAAYIQLSNIYACAGKWEESTEMRRKMKERNLVKEGGCSWIEVGDKIHKFYVGDT 734

Query: 618 MHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGQATHQNAIETEVVW 674
            HP    IY  L  L   +   GYVPD   +L+ L+++  D     +  +Q++ +  V +
Sbjct: 735 AHPNAHQIYDELDRLITEIKRCGYVPDTDLVLHKLEEE-NDEAEKERLLYQHSEKIAVAF 792


HSP 2 Score: 172.6 bits (436), Expect = 8.5e-43
Identity = 139/548 (25.36%), Postives = 260/548 (47.45%), Query Frame = 1

Query: 49  SFLLSICGREGNLHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFD 108
           S LL  C R  +  LG  +HA +I+ F++       V+ NSLIS+Y + G    A  VF+
Sbjct: 66  SSLLKSCIRARDFRLGKLVHARLIE-FDIEPDS---VLYNSLISLYSKSGDSAKAEDVFE 125

Query: 109 EMIT---RDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLE 168
            M     RD +SW+A++  +  NG+       F     +G    D    T ++ AC   +
Sbjct: 126 TMRRFGKRDVVSWSAMMACYGNNGRELDAIKVFVEFLELGLVPNDYC-YTAVIRACSNSD 185

Query: 169 FCWIIKMMHGLAFLSGYGQ-EITVGNALISSYFKC-GCVGLGMQVFYEMGERNVITWTAV 228
           F  + ++  G    +G+ + ++ VG +LI  + K         +VF +M E NV+TWT +
Sbjct: 186 FVGVGRVTLGFLMKTGHFESDVCVGCSLIDMFVKGENSFENAYKVFDKMSELNVVTWTLM 245

Query: 229 ISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLG 288
           I+   Q G+   +++ F +M+  G  E +  T  S+ +AC+ LE L  G Q+H   ++ G
Sbjct: 246 ITRCMQMGFPREAIRFFLDMVLSG-FESDKFTLSSVFSACAELENLSLGKQLHSWAIRSG 305

Query: 289 IQSDLCIGSALMDMYSK---SGRIGEAWKIFELAEELDMVSLTVILAGFTHN-GCEEEAI 348
           +  D  +  +L+DMY+K    G + +  K+F+  E+  ++S T ++ G+  N     EAI
Sbjct: 306 LVDD--VECSLVDMYAKCSADGSVDDCRKVFDRMEDHSVMSWTALITGYMKNCNLATEAI 365

Query: 349 QIFLKMLKMG-IEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINM 408
            +F +M+  G +E +    S      G  +  R+G+QV     K+    N  V+N +I+M
Sbjct: 366 NLFSEMITQGHVEPNHFTFSSAFKACGNLSDPRVGKQVLGQAFKRGLASNSSVANSVISM 425

Query: 409 YSKCGALDESMKVFDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTF 468
           + K   ++++ + F+ + E+N V++N+ +    R+ +  +A +L  ++       +  TF
Sbjct: 426 FVKSDRMEDAQRAFESLSEKNLVSYNTFLDGTCRNLNFEQAFKLLSEITERELGVSAFTF 485

Query: 469 LSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLP 528
            SLL   ++ G ++KG E + S     G++        ++ M  + G +  A   +    
Sbjct: 486 ASLLSGVANVGSIRKG-EQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASR-VFNFM 545

Query: 529 EQPGLLVWQALLGACSLYGDS-KIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERA 586
           E   ++ W +++   + +G + ++ +   + +      + V YV + +  S  G   E  
Sbjct: 546 ENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNEVTYVAILSACSHVGLVSEGW 603


HSP 3 Score: 167.9 bits (424), Expect = 2.1e-41
Identity = 134/519 (25.82%), Postives = 245/519 (47.21%), Query Frame = 1

Query: 150 DKATLTTILSACDGLEFCWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVF 209
           D  T +++L +C       + K++H          +  + N+LIS Y K G       VF
Sbjct: 61  DSVTFSSLLKSCIRARDFRLGKLVHARLIEFDIEPDSVLYNSLISLYSKSGDSAKAEDVF 120

Query: 210 YEM---GERNVITWTAVISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGL 269
             M   G+R+V++W+A+++    NG    ++K+F E +  G V PN   Y +++ ACS  
Sbjct: 121 ETMRRFGKRDVVSWSAMMACYGNNGRELDAIKVFVEFLELGLV-PNDYCYTAVIRACSNS 180

Query: 270 EALKEGCQIHGLIMKLG-IQSDLCIGSALMDMYSKSGRIGE-AWKIFELAEELDMVSLTV 329
           + +  G    G +MK G  +SD+C+G +L+DM+ K     E A+K+F+   EL++V+ T+
Sbjct: 181 DFVGVGRVTLGFLMKTGHFESDVCVGCSLIDMFVKGENSFENAYKVFDKMSELNVVTWTL 240

Query: 330 ILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKN 389
           ++      G   EAI+ FL M+  G E D   +S V        +L LG+Q+HS+ ++  
Sbjct: 241 MITRCMQMGFPREAIRFFLDMVLSGFESDKFTLSSVFSACAELENLSLGKQLHSWAIRSG 300

Query: 390 FICNPFVSNGLINMYSKC---GALDESMKVFDRMRERNSVTWNSMIAAFARHGD-ALKAL 449
            + +  V   L++MY+KC   G++D+  KVFDRM + + ++W ++I  + ++ + A +A+
Sbjct: 301 LVDD--VECSLVDMYAKCSADGSVDDCRKVFDRMEDHSVMSWTALITGYMKNCNLATEAI 360

Query: 450 QLYEDMQLEG-AKPTDVTFLSLLHACSHAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVD 509
            L+ +M  +G  +P   TF S   AC +    + G + L    K  G+   S     V+ 
Sbjct: 361 NLFSEMITQGHVEPNHFTFSSAFKACGNLSDPRVGKQVLGQAFK-RGLASNSSVANSVIS 420

Query: 510 MLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSKIGKYAAEHLFSETPDSPVP 569
           M  ++  + +A+   E L E+  +     L G C      +  K  +E    E   S   
Sbjct: 421 MFVKSDRMEDAQRAFESLSEKNLVSYNTFLDGTCRNLNFEQAFKLLSEITERELGVSAFT 480

Query: 570 YVLLANIYSSEGNWKERARTIRKMKEVGTAKETGI-----------SWIEIDKKVHSFTV 629
           +  L +  ++ G+ ++  +   ++ ++G +    +             I+   +V +F  
Sbjct: 481 FASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASRVFNFME 540

Query: 630 GDKMHPQTEMIYGV--------LWELFILMVDEGYVPDK 640
              +   T MI G         + E F  M++EG  P++
Sbjct: 541 NRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNE 575


HSP 4 Score: 105.1 bits (261), Expect = 1.7e-22
Identity = 77/301 (25.58%), Postives = 139/301 (46.18%), Query Frame = 1

Query: 10  RSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGNLHLGSSLHA 69
           ++ +L +   +L S    Q H  P          NH   S     CG   +  +G  +  
Sbjct: 348 KNCNLATEAINLFSEMITQGHVEP----------NHFTFSSAFKACGNLSDPRVGKQVLG 407

Query: 70  SIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALIGGFVRNG 129
              K    SN      + NS+ISM+ +  ++ DA + F+ +  ++ +S+N  + G  RN 
Sbjct: 408 QAFKRGLASNSS----VANSVISMFVKSDRMEDAQRAFESLSEKNLVSYNTFLDGTCRNL 467

Query: 130 KFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLSGYGQEITVG 189
            F   F     +    +      T  ++LS    +      + +H      G      V 
Sbjct: 468 NFEQAFKLLSEIT-ERELGVSAFTFASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVC 527

Query: 190 NALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLFKEMMSYGSV 249
           NALIS Y KCG +    +VF  M  RNVI+WT++I+G A++G+    L+ F +M+  G V
Sbjct: 528 NALISMYSKCGSIDTASRVFNFMENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEG-V 587

Query: 250 EPNSLTYLSLLTACSGLEALKEGCQ-IHGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAW 309
           +PN +TY+++L+ACS +  + EG +  + +     I+  +   + ++D+  ++G + +A+
Sbjct: 588 KPNEVTYVAILSACSHVGLVSEGWRHFNSMYEDHKIKPKMEHYACMVDLLCRAGLLTDAF 632

BLAST of Csa2G022820 vs. TAIR10
Match: AT3G57430.1 (AT3G57430.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 378.6 bits (971), Expect = 7.8e-105
Identity = 208/605 (34.38%), Postives = 334/605 (55.21%), Query Frame = 1

Query: 61  LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA 120
           L +G  +HA  ++  EL++      I+N+L++MY + GKL  +  +      RD ++WN 
Sbjct: 218 LMMGKQVHAYGLRKGELNSF-----IINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNT 277

Query: 121 LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS 180
           ++    +N +      Y + M L G    D+ T++++L AC  LE     K +H  A  +
Sbjct: 278 VLSSLCQNEQLLEALEYLREMVLEG-VEPDEFTISSVLPACSHLEMLRTGKELHAYALKN 337

Query: 181 GYGQEIT-VGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKL 240
           G   E + VG+AL+  Y  C  V  G +VF  M +R +  W A+I+G +QN + + +L L
Sbjct: 338 GSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLL 397

Query: 241 FKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYS 300
           F  M     +  NS T   ++ AC    A      IHG ++K G+  D  + + LMDMYS
Sbjct: 398 FIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYS 457

Query: 301 KSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKML-----------KM 360
           + G+I  A +IF   E+ D+V+   ++ G+  +   E+A+ +  KM            ++
Sbjct: 458 RLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRV 517

Query: 361 GIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDES 420
            ++ +   +  +L    A ++L  G+++H++ +K N   +  V + L++MY+KCG L  S
Sbjct: 518 SLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMS 577

Query: 421 MKVFDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHA 480
            KVFD++ ++N +TWN +I A+  HG+  +A+ L   M ++G KP +VTF+S+  ACSH+
Sbjct: 578 RKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHS 637

Query: 481 GLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLL-VWQ 540
           G+V +G+     M  D+G+ P S+H+ACVVD+LGRAG + EA   +  +P        W 
Sbjct: 638 GMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWS 697

Query: 541 ALLGACSLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVG 600
           +LLGA  ++ + +IG+ AA++L    P+    YVLLANIYSS G W +     R MKE G
Sbjct: 698 SLLGASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAGLWDKATEVRRNMKEQG 757

Query: 601 TAKETGISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLD 653
             KE G SWIE   +VH F  GD  HPQ+E + G L  L+  M  EGYVPD   +L+ ++
Sbjct: 758 VRKEPGCSWIEHGDEVHKFVAGDSSHPQSEKLSGYLETLWERMRKEGYVPDTSCVLHNVE 816


HSP 2 Score: 210.3 bits (534), Expect = 3.7e-54
Identity = 147/554 (26.53%), Postives = 269/554 (48.56%), Query Frame = 1

Query: 12  SHLPSWVTSLISPFRNQFHQNPFPETSSTFVL---------NHLDPSFLLSICGREGNLH 71
           S  P W   L+   R++   N   E   T+V          N+  P+ L ++   + ++ 
Sbjct: 59  SRSPEWWIDLL---RSKVRSNLLREAVLTYVDMIVLGIKPDNYAFPALLKAVADLQ-DME 118

Query: 72  LGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALI 131
           LG  +HA + K        + V + N+L+++Y +CG      KVFD +  R+ +SWN+LI
Sbjct: 119 LGKQIHAHVYK---FGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLI 178

Query: 132 GGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGL---EFCWIIKMMHGLAFL 191
                  K+      F+ M L  +      TL ++++AC  L   E   + K +H     
Sbjct: 179 SSLCSFEKWEMALEAFRCM-LDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLR 238

Query: 192 SGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKL 251
            G      + N L++ Y K G +     +    G R+++TW  V+S L QN     +L+ 
Sbjct: 239 KGELNSFII-NTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEY 298

Query: 252 FKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLG-IQSDLCIGSALMDMY 311
            +EM+  G VEP+  T  S+L ACS LE L+ G ++H   +K G +  +  +GSAL+DMY
Sbjct: 299 LREMVLEG-VEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMY 358

Query: 312 SKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKM-GIEIDGNVV 371
               ++    ++F+   +  +     ++AG++ N  ++EA+ +F+ M +  G+  +   +
Sbjct: 359 CNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTM 418

Query: 372 SVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRE 431
           + V+       +    + +H FVVK+    + FV N L++MYS+ G +D +M++F +M +
Sbjct: 419 AGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMED 478

Query: 432 RNSVTWNSMIAAFA---RHGDALKALQLYEDMQLE--------GAKPTDVTFLSLLHACS 491
           R+ VTWN+MI  +     H DAL  L   ++++ +          KP  +T +++L +C+
Sbjct: 479 RDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCA 538

Query: 492 HAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVW 541
               + KG E      K++     +   A +VDM  + G L  +R   +++P Q  ++ W
Sbjct: 539 ALSALAKGKEIHAYAIKNNLATDVAVGSA-LVDMYAKCGCLQMSRKVFDQIP-QKNVITW 598

BLAST of Csa2G022820 vs. TAIR10
Match: AT2G27610.1 (AT2G27610.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 377.9 bits (969), Expect = 1.3e-104
Identity = 201/605 (33.22%), Postives = 341/605 (56.36%), Query Frame = 1

Query: 52  LSICGREGNLHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMI 111
           L +   EG    G  +H  ++K+         + + NSLI++Y +CG +  A  +FD+  
Sbjct: 201 LGVLAEEGVGGRGLQVHTVVVKN----GLDKTIPVSNSLINLYLKCGNVRKARILFDKTE 260

Query: 112 TRDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIK 171
            +  ++WN++I G+  NG        F +M L    R  +++  +++  C  L+     +
Sbjct: 261 VKSVVTWNSMISGYAANGLDLEALGMFYSMRL-NYVRLSESSFASVIKLCANLKELRFTE 320

Query: 172 MMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMG-ERNVITWTAVISGLAQN 231
            +H      G+  +  +  AL+ +Y KC  +   +++F E+G   NV++WTA+ISG  QN
Sbjct: 321 QLHCSVVKYGFLFDQNIRTALMVAYSKCTAMLDALRLFKEIGCVGNVVSWTAMISGFLQN 380

Query: 232 GYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCI 291
              E ++ LF EM   G V PN  TY  +LTA   +       ++H  ++K   +    +
Sbjct: 381 DGKEEAVDLFSEMKRKG-VRPNEFTYSVILTALPVISP----SEVHAQVVKTNYERSSTV 440

Query: 292 GSALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGI 351
           G+AL+D Y K G++ EA K+F   ++ D+V+ + +LAG+   G  E AI++F ++ K GI
Sbjct: 441 GTALLDAYVKLGKVEEAAKVFSGIDDKDIVAWSAMLAGYAQTGETEAAIKMFGELTKGGI 500

Query: 352 EIDGNVVSVVLGVFGA-DTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESM 411
           + +    S +L V  A + S+  G+Q H F +K     +  VS+ L+ MY+K G ++ + 
Sbjct: 501 KPNEFTFSSILNVCAATNASMGQGKQFHGFAIKSRLDSSLCVSSALLTMYAKKGNIESAE 560

Query: 412 KVFDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAG 471
           +VF R RE++ V+WNSMI+ +A+HG A+KAL ++++M+    K   VTF+ +  AC+HAG
Sbjct: 561 EVFKRQREKDLVSWNSMISGYAQHGQAMKALDVFKEMKKRKVKMDGVTFIGVFAACTHAG 620

Query: 472 LVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQAL 531
           LV++G ++   M +D  + P  EH++C+VD+  RAG L +A   IE +P   G  +W+ +
Sbjct: 621 LVEEGEKYFDIMVRDCKIAPTKEHNSCMVDLYSRAGQLEKAMKVIENMPNPAGSTIWRTI 680

Query: 532 LGACSLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTA 591
           L AC ++  +++G+ AAE + +  P+    YVLL+N+Y+  G+W+ERA+  + M E    
Sbjct: 681 LAACRVHKKTELGRLAAEKIIAMKPEDSAAYVLLSNMYAESGDWQERAKVRKLMNERNVK 740

Query: 592 KETGISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDD 651
           KE G SWIE+  K +SF  GD+ HP  + IY  L +L   + D GY PD  ++L  +DD+
Sbjct: 741 KEPGYSWIEVKNKTYSFLAGDRSHPLKDQIYMKLEDLSTRLKDLGYEPDTSYVLQDIDDE 795

Query: 652 RRDPI 655
            ++ +
Sbjct: 801 HKEAV 795


HSP 2 Score: 180.3 bits (456), Expect = 4.1e-45
Identity = 136/487 (27.93%), Postives = 243/487 (49.90%), Query Frame = 1

Query: 64  GSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNALIG 123
           G  LH   IK   L +    V +  SL+  Y +     D  KVFDEM  R+ ++W  LI 
Sbjct: 112 GRQLHCQCIKFGFLDD----VSVGTSLVDTYMKGSNFKDGRKVFDEMKERNVVTWTTLIS 171

Query: 124 GFVRNGKFFAGFSYFKAMCLVGDC--RFDKATLTTILSACDGLEFCWIIKMMHGLAFLSG 183
           G+ RN       + F  M   G     F  A    +L+  +G+    +   +H +   +G
Sbjct: 172 GYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAE-EGVGGRGL--QVHTVVVKNG 231

Query: 184 YGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLFK 243
             + I V N+LI+ Y KCG V     +F +   ++V+TW ++ISG A NG    +L +F 
Sbjct: 232 LDKTIPVSNSLINLYLKCGNVRKARILFDKTEVKSVVTWNSMISGYAANGLDLEALGMFY 291

Query: 244 EM-MSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK 303
            M ++Y  +  +S  + S++  C+ L+ L+   Q+H  ++K G   D  I +ALM  YSK
Sbjct: 292 SMRLNYVRLSESS--FASVIKLCANLKELRFTEQLHCSVVKYGFLFDQNIRTALMVAYSK 351

Query: 304 SGRIGEAWKIF-ELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSV 363
              + +A ++F E+    ++VS T +++GF  N  +EEA+ +F +M + G+  +    SV
Sbjct: 352 CTAMLDALRLFKEIGCVGNVVSWTAMISGFLQNDGKEEAVDLFSEMKRKGVRPNEFTYSV 411

Query: 364 VLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERN 423
           +L      +      +VH+ VVK N+  +  V   L++ Y K G ++E+ KVF  + +++
Sbjct: 412 ILTALPVISP----SEVHAQVVKTNYERSSTVGTALLDAYVKLGKVEEAAKVFSGIDDKD 471

Query: 424 SVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGL-VKKGMEFL 483
            V W++M+A +A+ G+   A++++ ++   G KP + TF S+L+ C+     + +G +F 
Sbjct: 472 IVAWSAMLAGYAQTGETEAAIKMFGELTKGGIKPNEFTFSSILNVCAATNASMGQGKQF- 531

Query: 484 KSMTKDHG--MNPRSEHHACV----VDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGA 540
                 HG  +  R +   CV    + M  + G +  A    ++  E+  L+ W +++  
Sbjct: 532 ------HGFAIKSRLDSSLCVSSALLTMYAKKGNIESAEEVFKRQREK-DLVSWNSMISG 577


HSP 3 Score: 173.7 bits (439), Expect = 3.8e-43
Identity = 123/465 (26.45%), Postives = 231/465 (49.68%), Query Frame = 1

Query: 99  KLPDAVKVFDEMITRDTISWNALIGGFVRNGKFFAGFSYFKAMCLVG---DCRFDKATLT 158
           +L +A  +FD+   RD  S+ +L+ GF R+G+       F  +  +G   DC    + L 
Sbjct: 42  RLYNAHNLFDKSPGRDRESYISLLFGFSRDGRTQEAKRLFLNIHRLGMEMDCSIFSSVLK 101

Query: 159 TILSACDGLEFCWIIKMMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGER 218
              + CD L      + +H      G+  +++VG +L+ +Y K      G +VF EM ER
Sbjct: 102 VSATLCDEL----FGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDGRKVFDEMKER 161

Query: 219 NVITWTAVISGLAQNGYHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQI 278
           NV+TWT +ISG A+N  ++  L LF  M + G+ +PNS T+ + L   +       G Q+
Sbjct: 162 NVVTWTTLISGYARNSMNDEVLTLFMRMQNEGT-QPNSFTFAAALGVLAEEGVGGRGLQV 221

Query: 279 HGLIMKLGIQSDLCIGSALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCE 338
           H +++K G+   + + ++L+++Y K G + +A  +F+  E   +V+   +++G+  NG +
Sbjct: 222 HTVVVKNGLDKTIPVSNSLINLYLKCGNVRKARILFDKTEVKSVVTWNSMISGYAANGLD 281

Query: 339 EEAIQIFLKMLKMGIEIDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGL 398
            EA+ +F  M    + +  +  + V+ +      LR  +Q+H  VVK  F+ +  +   L
Sbjct: 282 LEALGMFYSMRLNYVRLSESSFASVIKLCANLKELRFTEQLHCSVVKYGFLFDQNIRTAL 341

Query: 399 INMYSKCGALDESMKVFDRMR-ERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPT 458
           +  YSKC A+ +++++F  +    N V+W +MI+ F ++    +A+ L+ +M+ +G +P 
Sbjct: 342 MVAYSKCTAMLDALRLFKEIGCVGNVVSWTAMISGFLQNDGKEEAVDLFSEMKRKGVRPN 401

Query: 459 DVTFLSLLHACS-------HAGLVKKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGML 518
           + T+  +L A         HA +VK   E  +S T              ++D   + G +
Sbjct: 402 EFTYSVILTALPVISPSEVHAQVVKTNYE--RSSTVG----------TALLDAYVKLGKV 461

Query: 519 SEARNFIEKLPEQPGLLVWQALLGACSLYGDSKIGKYAAEHLFSE 553
            EA      + ++  ++ W A+L   +  G+++    AA  +F E
Sbjct: 462 EEAAKVFSGIDDK-DIVAWSAMLAGYAQTGETE----AAIKMFGE 484

BLAST of Csa2G022820 vs. NCBI nr
Match: gi|700205794|gb|KGN60913.1| (hypothetical protein Csa_2G022820 [Cucumis sativus])

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 674/674 (100.00%), Postives = 674/674 (100.00%), Query Frame = 1

Query: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN 60
           MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN
Sbjct: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN 60

Query: 61  LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA 120
           LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA
Sbjct: 61  LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA 120

Query: 121 LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS 180
           LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS
Sbjct: 121 LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS 180

Query: 181 GYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLF 240
           GYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLF
Sbjct: 181 GYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLF 240

Query: 241 KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK 300
           KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK
Sbjct: 241 KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK 300

Query: 301 SGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVV 360
           SGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVV
Sbjct: 301 SGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVV 360

Query: 361 LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS 420
           LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS
Sbjct: 361 LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS 420

Query: 421 VTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS 480
           VTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS
Sbjct: 421 VTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS 480

Query: 481 MTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK 540
           MTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK
Sbjct: 481 MTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK 540

Query: 541 IGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEID 600
           IGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEID
Sbjct: 541 IGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEID 600

Query: 601 KKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGQAT 660
           KKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGQAT
Sbjct: 601 KKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGQAT 660

Query: 661 HQNAIETEVVWELF 675
           HQNAIETEVVWELF
Sbjct: 661 HQNAIETEVVWELF 674

BLAST of Csa2G022820 vs. NCBI nr
Match: gi|778666838|ref|XP_011648824.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g05340 [Cucumis sativus])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 658/663 (99.25%), Postives = 658/663 (99.25%), Query Frame = 1

Query: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN 60
           MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN
Sbjct: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN 60

Query: 61  LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA 120
           LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA
Sbjct: 61  LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA 120

Query: 121 LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS 180
           LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS
Sbjct: 121 LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS 180

Query: 181 GYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLF 240
           GYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLF
Sbjct: 181 GYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLF 240

Query: 241 KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK 300
           KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK
Sbjct: 241 KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK 300

Query: 301 SGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVV 360
           SGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVV
Sbjct: 301 SGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVV 360

Query: 361 LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS 420
           LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS
Sbjct: 361 LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS 420

Query: 421 VTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS 480
           VTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS
Sbjct: 421 VTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS 480

Query: 481 MTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK 540
           MTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK
Sbjct: 481 MTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK 540

Query: 541 IGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEID 600
           IGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEID
Sbjct: 541 IGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEID 600

Query: 601 KKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGQAT 660
           KKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNG   
Sbjct: 601 KKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHNGHND 660

Query: 661 HQN 664
             N
Sbjct: 661 TSN 663

BLAST of Csa2G022820 vs. NCBI nr
Match: gi|659070327|ref|XP_008454568.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g05340 [Cucumis melo])

HSP 1 Score: 1262.3 bits (3265), Expect = 0.0e+00
Identity = 624/656 (95.12%), Postives = 639/656 (97.41%), Query Frame = 1

Query: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGREGN 60
           MKLKWVFQK SSHLPS VTSLI PFRNQFHQNPF ETSSTFVLNHLD SFLLSICGREGN
Sbjct: 1   MKLKWVFQKSSSHLPSLVTSLIFPFRNQFHQNPFAETSSTFVLNHLDVSFLLSICGREGN 60

Query: 61  LHLGSSLHASIIKSFELSNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMITRDTISWNA 120
           LHLGSSLHASIIKSFE SNHYNGVVIMNSLISMYDRCGKL DAVKVFDEM+TRDTISWNA
Sbjct: 61  LHLGSSLHASIIKSFEPSNHYNGVVIMNSLISMYDRCGKLSDAVKVFDEMLTRDTISWNA 120

Query: 121 LIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMMHGLAFLS 180
           LIGGFVRNGKFFAGFSYFKAMCLVGDC+FDKATLTTILSACDGLEFC IIKMMHGLAFLS
Sbjct: 121 LIGGFVRNGKFFAGFSYFKAMCLVGDCKFDKATLTTILSACDGLEFCCIIKMMHGLAFLS 180

Query: 181 GYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYHEHSLKLF 240
           G+GQEITVGNAL+SSY KCGCVGLGMQVF EMGERNVITWTAVISGLA+NG+HEHSLKLF
Sbjct: 181 GFGQEITVGNALVSSYLKCGCVGLGMQVFDEMGERNVITWTAVISGLARNGHHEHSLKLF 240

Query: 241 KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSALMDMYSK 300
           KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLI+KLGIQSDLCIGSALMDMYSK
Sbjct: 241 KEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLILKLGIQSDLCIGSALMDMYSK 300

Query: 301 SGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEIDGNVVSVV 360
           SGRIGEAWKIFE AEELDMVSLTVILAGFT NGCEEEAIQIFLKMLKMGIEIDGNVVSVV
Sbjct: 301 SGRIGEAWKIFESAEELDMVSLTVILAGFTQNGCEEEAIQIFLKMLKMGIEIDGNVVSVV 360

Query: 361 LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS 420
           LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS
Sbjct: 361 LGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFDRMRERNS 420

Query: 421 VTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS 480
           VTWNSMIAAFARHGDA KALQLYE+MQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS
Sbjct: 421 VTWNSMIAAFARHGDASKALQLYENMQLEGAKPTDVTFLSLLHACSHAGLVKKGMEFLKS 480

Query: 481 MTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSK 540
           MTKDHGMNPRSEH+ACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDS+
Sbjct: 481 MTKDHGMNPRSEHYACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGACSLYGDSE 540

Query: 541 IGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETGISWIEID 600
           +GKYAA+HLF ETP S VPYVLLANIYSSEGNWKERARTIR+MKEVGTAKETGISWIEID
Sbjct: 541 MGKYAADHLFLETPHSTVPYVLLANIYSSEGNWKERARTIRRMKEVGTAKETGISWIEID 600

Query: 601 KKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRRDPIHN 657
           KKVHSFTVGDKMHPQTE+IYGVL ELF+LMVDEGYVPDKKFILYYLDDDRRDPIHN
Sbjct: 601 KKVHSFTVGDKMHPQTEIIYGVLTELFVLMVDEGYVPDKKFILYYLDDDRRDPIHN 656

BLAST of Csa2G022820 vs. NCBI nr
Match: gi|1009117073|ref|XP_015875118.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g05340 [Ziziphus jujuba])

HSP 1 Score: 892.5 bits (2305), Expect = 4.6e-256
Identity = 453/651 (69.59%), Postives = 532/651 (81.72%), Query Frame = 1

Query: 1   MKLKWVFQKRSSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHL-DPSFLLSICGREG 60
           MK  WVF+K     PSWV+SL+  FR    QNP  ETS  FVL+H  D S L+SICGREG
Sbjct: 1   MKPSWVFRKLKEPSPSWVSSLLFHFRTGICQNPSLETSR-FVLDHAEDISLLISICGREG 60

Query: 61  NLHLGSSLHASIIKSFE---LSNHY---NGVVIMNSLISMYDRCGKLPDAVKVFDEMITR 120
            +HLGSSLHASIIK FE   L N Y   + +VI NSL+SMY RCG+L DAVK+FDE+  +
Sbjct: 61  YVHLGSSLHASIIKHFEFFNLDNRYVPRDVIVIWNSLLSMYSRCGRLFDAVKLFDEIPMK 120

Query: 121 DTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIKMM 180
           DT+SWN +I GF+RNG+F  GF YFK M  +G  RFDKATLTTILSA DG EFC++ KM+
Sbjct: 121 DTVSWNTMISGFLRNGEFEVGFGYFKRMRQLGFHRFDKATLTTILSALDGPEFCYLNKMI 180

Query: 181 HGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNGYH 240
           HGL FL+GY  E+TVGNALI+SY KCGC   G +VF  M ERNVITWTA+ISGLAQN ++
Sbjct: 181 HGLVFLNGYEGEVTVGNALITSYCKCGCFSSGRRVFDGMFERNVITWTAMISGLAQNEFY 240

Query: 241 EHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIGSA 300
           E SLKLF  MM  G+ EPNSLTYLS L A SGL+AL EG QIH L+ K GIQSDLCI SA
Sbjct: 241 EESLKLFV-MMRCGTSEPNSLTYLSSLMASSGLQALSEGRQIHALLWKQGIQSDLCIESA 300

Query: 301 LMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIEID 360
           LMDMYSK G +G+AW+IFE AEELD VS+TVIL GF  NG EEEAIQIF +M+K GIEID
Sbjct: 301 LMDMYSKCGSVGDAWQIFESAEELDEVSMTVILVGFAQNGFEEEAIQIFKRMVKAGIEID 360

Query: 361 GNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKVFD 420
            N+VS +LGVF  DTSL LG+Q+HS ++KKNF  NP+VSNGLINMYSKCG LDES+KVF+
Sbjct: 361 PNMVSAILGVFSVDTSLGLGKQIHSLIIKKNFGFNPYVSNGLINMYSKCGELDESVKVFN 420

Query: 421 RMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLVKK 480
           RM +RNS++WNSMIAAFARHGD L+ALQLYE+M+LEG + TDVTFLSLLHACSH GLV+K
Sbjct: 421 RMPQRNSISWNSMIAAFARHGDGLRALQLYEEMKLEGVQQTDVTFLSLLHACSHVGLVEK 480

Query: 481 GMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLGAC 540
           G+EFL SM KD G++PR+EH+A VVDMLGRAG+L+EA++FIE LPE+PG LVWQALLGAC
Sbjct: 481 GLEFLNSMAKDIGISPRAEHYASVVDMLGRAGLLTEAKSFIEGLPEKPGPLVWQALLGAC 540

Query: 541 SLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKETG 600
           S++GD ++GKYAAE LFS TP+SP PY+LLANIYSS+G WKERARTI++MKE+G AKETG
Sbjct: 541 SIHGDPEMGKYAAEQLFSATPESPAPYILLANIYSSKGKWKERARTIKRMKEMGVAKETG 600

Query: 601 ISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILY 645
           ISWIEI+KKVHSF VGD++HPQ E+IY VL ELF LM DEGYVPD+KFILY
Sbjct: 601 ISWIEIEKKVHSFVVGDRLHPQVEIIYAVLSELFRLMTDEGYVPDEKFILY 649

BLAST of Csa2G022820 vs. NCBI nr
Match: gi|657992620|ref|XP_008388566.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g05340 [Malus domestica])

HSP 1 Score: 875.2 bits (2260), Expect = 7.6e-251
Identity = 436/660 (66.06%), Postives = 523/660 (79.24%), Query Frame = 1

Query: 1   MKLKWVFQKR---SSHLPSWVTSLISPFRNQFHQNPFPETSSTFVLNHLDPSFLLSICGR 60
           MK  WVFQ +   S H PS  +SLIS F+ + H+NP  +T++T VLN++D S LLS+CG+
Sbjct: 1   MKSWWVFQLQNLSSHHFPSCASSLISAFKTKLHRNPDSQTTATLVLNNVDISLLLSLCGK 60

Query: 61  EGNLHLGSSLHASIIKSFEL------SNHYNGVVIMNSLISMYDRCGKLPDAVKVFDEMI 120
           + N  LGSSLHAS+IK+ E        +H N +V+ NSL+SMY +CGKL +AV++FD+M 
Sbjct: 61  DRNFQLGSSLHASLIKNPEFFHPENHDDHRNALVVWNSLLSMYLKCGKLRNAVQLFDDMR 120

Query: 121 TRDTISWNALIGGFVRNGKFFAGFSYFKAMCLVGDCRFDKATLTTILSACDGLEFCWIIK 180
            RDT+SWN +I GF+RNG+   GF YFK MC +G  RFDKATLT+IL+A DG EFC + K
Sbjct: 121 VRDTVSWNTMISGFLRNGELDYGFGYFKQMCGLGCYRFDKATLTSILAAFDGPEFCHLNK 180

Query: 181 MMHGLAFLSGYGQEITVGNALISSYFKCGCVGLGMQVFYEMGERNVITWTAVISGLAQNG 240
           MMHGL  L+G+ +E  VGNALI+SY KCGC   G +VF EM ERNVITWTA+ISGLAQN 
Sbjct: 181 MMHGLVVLNGFERETAVGNALITSYCKCGCFLSGRRVFDEMFERNVITWTAMISGLAQNE 240

Query: 241 YHEHSLKLFKEMMSYGSVEPNSLTYLSLLTACSGLEALKEGCQIHGLIMKLGIQSDLCIG 300
           Y+  SL+LF EM   G V+PNS+TYL LL ACSGL+A+  G QIHGL  KLGIQS+LCI 
Sbjct: 241 YYVESLELFLEMRG-GVVDPNSMTYLGLLMACSGLQAISVGRQIHGLAWKLGIQSELCIE 300

Query: 301 SALMDMYSKSGRIGEAWKIFELAEELDMVSLTVILAGFTHNGCEEEAIQIFLKMLKMGIE 360
           SALMDMYSK G + +AWKIFE  EELD +S+TVIL GF  NG E+EAI IF+KM+K GI+
Sbjct: 301 SALMDMYSKCGSVEDAWKIFESTEELDEISMTVILVGFAQNGFEDEAIHIFVKMIKAGID 360

Query: 361 IDGNVVSVVLGVFGADTSLRLGQQVHSFVVKKNFICNPFVSNGLINMYSKCGALDESMKV 420
           ID N+VS VLGVF  DTSL LG+Q+HS +VKKNF  N FV NGLINMYSKCG L++S+KV
Sbjct: 361 IDPNMVSAVLGVFXVDTSLGLGKQLHSLIVKKNFGSNSFVGNGLINMYSKCGELEDSVKV 420

Query: 421 FDRMRERNSVTWNSMIAAFARHGDALKALQLYEDMQLEGAKPTDVTFLSLLHACSHAGLV 480
           F RM +RNS++WNSMIAAFARHGD  KALQLYE M +EG +PTDVTFLSLLHACSH G V
Sbjct: 421 FSRMPQRNSISWNSMIAAFARHGDGSKALQLYEKMNMEGVQPTDVTFLSLLHACSHVGFV 480

Query: 481 KKGMEFLKSMTKDHGMNPRSEHHACVVDMLGRAGMLSEARNFIEKLPEQPGLLVWQALLG 540
           ++GMEFLKSM +DHGM+PR EH+AC VDMLGRAG L+EA++FIEKLPE PG+LVWQALLG
Sbjct: 481 ERGMEFLKSMNEDHGMSPRPEHYACXVDMLGRAGHLTEAKSFIEKLPENPGVLVWQALLG 540

Query: 541 ACSLYGDSKIGKYAAEHLFSETPDSPVPYVLLANIYSSEGNWKERARTIRKMKEVGTAKE 600
           AC ++GDS+IGKYAA+ L    P++P PYVLLANIYSSEG WKERARTI+ MKE+G  KE
Sbjct: 541 ACCIHGDSEIGKYAADQLLLAAPETPAPYVLLANIYSSEGRWKERARTIKGMKEMGVTKE 600

Query: 601 TGISWIEIDKKVHSFTVGDKMHPQTEMIYGVLWELFILMVDEGYVPDKKFILYYLDDDRR 652
           TGISWIEI+KKV SF VGD+MHPQ E IY VL ELF LM DEGYVPD++FILYYLD D +
Sbjct: 601 TGISWIEIEKKVQSFVVGDRMHPQAEPIYRVLGELFRLMTDEGYVPDERFILYYLDQDEK 659

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP215_ARATH6.7e-21558.70Pentatricopeptide repeat-containing protein At3g05340 OS=Arabidopsis thaliana GN... [more]
PP296_ARATH3.9e-10633.99Pentatricopeptide repeat-containing protein At3g63370, chloroplastic OS=Arabidop... [more]
PP373_ARATH5.1e-10637.48Putative pentatricopeptide repeat-containing protein At5g09950 OS=Arabidopsis th... [more]
PP272_ARATH2.8e-10435.71Pentatricopeptide repeat-containing protein At3g49170, chloroplastic OS=Arabidop... [more]
PP285_ARATH1.4e-10334.38Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A0A0LGC8_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G022820 PE=4 SV=1[more]
B9I7R7_POPTR4.8e-24465.39Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s02200g PE=4 SV=2[more]
M5WNH2_PRUPE2.0e-24266.00Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023606mg PE=4 SV=1[more]
A0A0R0KNE9_SOYBN1.3e-24166.88Uncharacterized protein OS=Glycine max GN=GLYMA_03G238000 PE=4 SV=1[more]
A0A0L9VDX2_PHAAN2.7e-23963.40Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan09g152300 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G05340.13.8e-21658.70 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT5G09950.12.9e-10737.48 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT3G49170.11.6e-10535.71 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT3G57430.17.8e-10534.38 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT2G27610.11.3e-10433.22 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|700205794|gb|KGN60913.1|0.0e+00100.00hypothetical protein Csa_2G022820 [Cucumis sativus][more]
gi|778666838|ref|XP_011648824.1|0.0e+0099.25PREDICTED: pentatricopeptide repeat-containing protein At3g05340 [Cucumis sativu... [more]
gi|659070327|ref|XP_008454568.1|0.0e+0095.12PREDICTED: pentatricopeptide repeat-containing protein At3g05340 [Cucumis melo][more]
gi|1009117073|ref|XP_015875118.1|4.6e-25669.59PREDICTED: pentatricopeptide repeat-containing protein At3g05340 [Ziziphus jujub... [more]
gi|657992620|ref|XP_008388566.1|7.6e-25166.06PREDICTED: pentatricopeptide repeat-containing protein At3g05340 [Malus domestic... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
IPR011990TPR-like_helical_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa2G022820.1Csa2G022820.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 320..350
score: 0.0011coord: 292..314
score: 0.046coord: 116..142
score: 3.9E-4coord: 85..113
score: 0.
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 419..466
score: 1.6E-12coord: 216..263
score: 1.8
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 421..454
score: 6.3E-8coord: 116..142
score: 5.2E-4coord: 218..252
score: 2.4E-7coord: 321..353
score: 2.
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 287..317
score: 7.311coord: 454..489
score: 8.232coord: 216..250
score: 10.654coord: 83..117
score: 9.229coord: 318..352
score: 9.471coord: 252..286
score: 7.037coord: 556..590
score: 7.289coord: 490..520
score: 5.974coord: 419..453
score: 12.737coord: 388..418
score: 9.799coord: 185..215
score: 6
IPR011990Tetratricopeptide-like helical domainGENE3DG3DSA:1.25.40.10coord: 554..586
score: 3.3E-11coord: 382..463
score: 3.3E-11coord: 214..348
score: 3.3E-11coord: 85..114
score: 3.3
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 64..597
score: 0.0coord: 3..24
score:
NoneNo IPR availablePANTHERPTHR24015:SF563SUBFAMILY NOT NAMEDcoord: 3..24
score: 0.0coord: 64..597
score: