Csa1G614650 (gene) Cucumber (Chinese Long) v2

NameCsa1G614650
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionPhospholipase A1; contains IPR002921 (Lipase, class 3)
LocationChr1 : 24213385 .. 24216340 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCCCTCCACACCCTCCTCTCTCTCTCTATCCATTAAATTAAAATTTGATCTCTCTCTCTCAAATGCATTATTCCTCTCTCTCACCTGAAATCTAGAATATTCAGAGATCTCAAACCCTCCCTCTCCATGTCCATGGCTACAACTTCTTCTCTTCTACATTTTTCCACCATTCCTATTCCCATCTTCACAAAACTCCCCAAATATCACCTTGTCCATACCAACATGATGAACAATAACAACAATTCCCTTTTCTCCACAAAACCCACTTTTTCAATACATTACTTCAATTTCACTCTTCCAACCTCAAAATGCTCTTCATCATTACCTTCTTTAACAACAATGGAAGATCAAGAAGATGAAGATCAAGATGATGATGAAGCGATAACAGTGGAAACAAATAATAATTTTCCTCCACTTAGTGAAATTTGGAGAGACATTCAAGGGATGAACAATTGGGAAGCCCTTCTTGACCCTTTGAATCTTCATCTTCGTAAAGAAATCATTCGTTATGGTGAATTCTCTCAAGCTTGCTATGACTCTTTTGATTTTGATCCTCACTCTAAGTATTGTGGCACTTGCAAGTACCAAGCTTCCCACTTCTTTAACAAGCTTCTCATGCCTGATCCCGGCTACAATATCACTAGATATCTCTACGCAACCTCCAACATCAATCTACCCAACTTCTTTAAAAAATCCAAATTTACCCGTGATGAATCCAGAGACTTTTCTAACAGGTGAAATTTTCCGATGTTATTATAAGTTTGGTATCTAAACTTTCAAAACATCTTTCTGCAAATACTTCTACAAACTTTTAAGATAATGTTGATCATAAGAATTAGTAAGTACCAATTTAATTAGATCTTCAAACTTTTAAATTTATATCTATGAATTTAAGAAGTTGTTTGATGGTTCTATACAAGTTTACTCCTTAAACTTTTAGAGTTGTGTCAAACAGATTCTTAAACTAGTTAATACAAAATTTTTAGAGGTTTCAAAAGTGTTGCTAAGCTATAATATGCCTTAGATACATACTCATTTTTTTTTATTAAGATTCACAAGTTTGTTAGACACGAAATTAAATTTTATATATAATAAATTTGTTATTTTATTTTTTAGAAAATATTTGAATATGTTTGAGCTTATTAGACACAAAATAAAAAATCTATAGAACTATTTAAGTTATTGAAATTTTAAAGACTACCTATTTGATACTTTTTGAAGTTCAAAGAAGATACGAAATATATGAAAAGTTGTTGGACCAGCACATGTAATTTAACTTTTTTACATATATCGATGATTAGAGTTTGGAGTCAACATGCTAATTGGATGGGCTACGTCGCCGTTGCCACCGATGCCGATGAGATCAAACGACTTGGCCGCCGTGACATTGTCATAGCTTGGAGAGGAACTGTAACATACCTTGAATGGATCCATGACCTCAAAGACATTCTTCGACCGGCCGGTTTTATTCCAGATGACACGTCGGTTAAGATAGAGTCTGGATTCTATGATTTGTACACCATGAAAGAGGAAAATTGCAACTACTGTGGATTTTCAGCTCGTGAGCAGATTTTAGCTGAAGTTAAACGACTTGTGGAGAAGTATTGCAAATTGGGAGGAGAAGAAATTAGCATCACCGTTACTGGCCATAGTCTTGGAGCTGCTTTGGCTTTGATTACAGCCTATGACATTGCTGAGATGAAGCTGAACATTGTCGCCAGAGGAGGTAGCAGTGCCGTGGCAGTTCCGATCACTGTCTATTCATTTGCTGGTCCTCGGGTGGGGAATCTCAAGTTTAAGGAACGGTGCGAGGAGCTTGGAGTTAAAGTGTTGCGAGTGATTAATGTTCATGACAAGGTTATGATCAACTAAACTCAAAAGCTTAAGATGGTAGACGTGTTTTTGATTTGCAGGTTGAATTAGTATAACTTGTAACACTTTTTCTCACTTTTTGGATATAGCAATTAATTTGTCAATCTAAATATAACTTAACATTAGACAATTTTTTTTTTTAAAAGTGAAACCTTTTAATCCTTACCATAAAATCATTGTAGGTTACATGGTTTTATATCATATTTTTCTGGTTAAATTTTTTATTTTCATGGTTTGGAAAAGGTTGGTATTTAATCCCTATGGTTTTAAAAGTTCAACTCTAGCCCATTTAATTTTGATAAAACTTTGTAAATAATTCTATTATGATTTGATAAGAGATTCATAAATATAATTTTATAAAGCTTGGACTAAATGTCTTTTTGGTATTCACATGTGTGACAGGTTCCAATGGTACCAGGCATTATAGCCAATGAAAAGCTACAATTCCAAAAATATTTAGAAGAAGCTATAGCTTTTCCTTGGAGCTGTGCTCATGTTGGCACAGAACTTGCATTGGACCACACACACAGCCCCTTTCTAATGTCGACAAGCGATCTTGGGTGTGCTCACAATCTTGAAGCTTACCTACACTTAATCGATGGGTACCACGGTAAGGGGCGAAAGTTTCGACTAGAGACAAAGAGAGACATAGCTTTAGTGAACAAGAGTTGTGACTTTTTGAGAAAGGAGTACGGTGTGCCACCGTGTTGGCGACAAGACGAAAATAAAGGGATGGTTAGAAACAATGAAGGGCGGTGGGTGGTGCCAGATAGACCGAGGCTAGAGGATCATCCGCCTGATACTGCACACCACCTTCAACAAGTGCTCAAAGATGTTTTTGGTTCATAGGATTTAGGGTTTACAATCAAGATAGCTAGTGATAGCCAGCTTTATTTCATCATGGAGGGTAGGTTGAATGACTTGTTGACTAAATAATTAATAATGTCCTTGTTGATGAAGAGTGGCATTATTTTGGTGTCACTAATGTAAATTATCATGTGAATTGTATGGTATATATTTTGTTGACGATGAGTGGCATATATGATACTATTTTGCTTTTGAATATGAAATAAGTTTTGGCC

mRNA sequence

ATGTCCATGGCTACAACTTCTTCTCTTCTACATTTTTCCACCATTCCTATTCCCATCTTCACAAAACTCCCCAAATATCACCTTGTCCATACCAACATGATGAACAATAACAACAATTCCCTTTTCTCCACAAAACCCACTTTTTCAATACATTACTTCAATTTCACTCTTCCAACCTCAAAATGCTCTTCATCATTACCTTCTTTAACAACAATGGAAGATCAAGAAGATGAAGATCAAGATGATGATGAAGCGATAACAGTGGAAACAAATAATAATTTTCCTCCACTTAGTGAAATTTGGAGAGACATTCAAGGGATGAACAATTGGGAAGCCCTTCTTGACCCTTTGAATCTTCATCTTCGTAAAGAAATCATTCGTTATGGTGAATTCTCTCAAGCTTGCTATGACTCTTTTGATTTTGATCCTCACTCTAAGTATTGTGGCACTTGCAAGTACCAAGCTTCCCACTTCTTTAACAAGCTTCTCATGCCTGATCCCGGCTACAATATCACTAGATATCTCTACGCAACCTCCAACATCAATCTACCCAACTTCTTTAAAAAATCCAAATTTACCCGTGATGAATCCAGAGACTTTTCTAACAGAGTTTGGAGTCAACATGCTAATTGGATGGGCTACGTCGCCGTTGCCACCGATGCCGATGAGATCAAACGACTTGGCCGCCGTGACATTGTCATAGCTTGGAGAGGAACTGTAACATACCTTGAATGGATCCATGACCTCAAAGACATTCTTCGACCGGCCGGTTTTATTCCAGATGACACGTCGGTTAAGATAGAGTCTGGATTCTATGATTTGTACACCATGAAAGAGGAAAATTGCAACTACTGTGGATTTTCAGCTCGTGAGCAGATTTTAGCTGAAGTTAAACGACTTGTGGAGAAGTATTGCAAATTGGGAGGAGAAGAAATTAGCATCACCGTTACTGGCCATAGTCTTGGAGCTGCTTTGGCTTTGATTACAGCCTATGACATTGCTGAGATGAAGCTGAACATTGTCGCCAGAGGAGGTAGCAGTGCCGTGGCAGTTCCGATCACTGTCTATTCATTTGCTGGTCCTCGGGTGGGGAATCTCAAGTTTAAGGAACGGTGCGAGGAGCTTGGAGTTAAAGTGTTGCGAGTGATTAATGTTCATGACAAGGTTCCAATGGTACCAGGCATTATAGCCAATGAAAAGCTACAATTCCAAAAATATTTAGAAGAAGCTATAGCTTTTCCTTGGAGCTGTGCTCATGTTGGCACAGAACTTGCATTGGACCACACACACAGCCCCTTTCTAATGTCGACAAGCGATCTTGGGTGTGCTCACAATCTTGAAGCTTACCTACACTTAATCGATGGGTACCACGGTAAGGGGCGAAAGTTTCGACTAGAGACAAAGAGAGACATAGCTTTAGTGAACAAGAGTTGTGACTTTTTGAGAAAGGAGTACGGTGTGCCACCGTGTTGGCGACAAGACGAAAATAAAGGGATGGTTAGAAACAATGAAGGGCGGTGGGTGGTGCCAGATAGACCGAGGCTAGAGGATCATCCGCCTGATACTGCACACCACCTTCAACAAGTGCTCAAAGATGTTTTTGGTTCATAG

Coding sequence (CDS)

ATGTCCATGGCTACAACTTCTTCTCTTCTACATTTTTCCACCATTCCTATTCCCATCTTCACAAAACTCCCCAAATATCACCTTGTCCATACCAACATGATGAACAATAACAACAATTCCCTTTTCTCCACAAAACCCACTTTTTCAATACATTACTTCAATTTCACTCTTCCAACCTCAAAATGCTCTTCATCATTACCTTCTTTAACAACAATGGAAGATCAAGAAGATGAAGATCAAGATGATGATGAAGCGATAACAGTGGAAACAAATAATAATTTTCCTCCACTTAGTGAAATTTGGAGAGACATTCAAGGGATGAACAATTGGGAAGCCCTTCTTGACCCTTTGAATCTTCATCTTCGTAAAGAAATCATTCGTTATGGTGAATTCTCTCAAGCTTGCTATGACTCTTTTGATTTTGATCCTCACTCTAAGTATTGTGGCACTTGCAAGTACCAAGCTTCCCACTTCTTTAACAAGCTTCTCATGCCTGATCCCGGCTACAATATCACTAGATATCTCTACGCAACCTCCAACATCAATCTACCCAACTTCTTTAAAAAATCCAAATTTACCCGTGATGAATCCAGAGACTTTTCTAACAGAGTTTGGAGTCAACATGCTAATTGGATGGGCTACGTCGCCGTTGCCACCGATGCCGATGAGATCAAACGACTTGGCCGCCGTGACATTGTCATAGCTTGGAGAGGAACTGTAACATACCTTGAATGGATCCATGACCTCAAAGACATTCTTCGACCGGCCGGTTTTATTCCAGATGACACGTCGGTTAAGATAGAGTCTGGATTCTATGATTTGTACACCATGAAAGAGGAAAATTGCAACTACTGTGGATTTTCAGCTCGTGAGCAGATTTTAGCTGAAGTTAAACGACTTGTGGAGAAGTATTGCAAATTGGGAGGAGAAGAAATTAGCATCACCGTTACTGGCCATAGTCTTGGAGCTGCTTTGGCTTTGATTACAGCCTATGACATTGCTGAGATGAAGCTGAACATTGTCGCCAGAGGAGGTAGCAGTGCCGTGGCAGTTCCGATCACTGTCTATTCATTTGCTGGTCCTCGGGTGGGGAATCTCAAGTTTAAGGAACGGTGCGAGGAGCTTGGAGTTAAAGTGTTGCGAGTGATTAATGTTCATGACAAGGTTCCAATGGTACCAGGCATTATAGCCAATGAAAAGCTACAATTCCAAAAATATTTAGAAGAAGCTATAGCTTTTCCTTGGAGCTGTGCTCATGTTGGCACAGAACTTGCATTGGACCACACACACAGCCCCTTTCTAATGTCGACAAGCGATCTTGGGTGTGCTCACAATCTTGAAGCTTACCTACACTTAATCGATGGGTACCACGGTAAGGGGCGAAAGTTTCGACTAGAGACAAAGAGAGACATAGCTTTAGTGAACAAGAGTTGTGACTTTTTGAGAAAGGAGTACGGTGTGCCACCGTGTTGGCGACAAGACGAAAATAAAGGGATGGTTAGAAACAATGAAGGGCGGTGGGTGGTGCCAGATAGACCGAGGCTAGAGGATCATCCGCCTGATACTGCACACCACCTTCAACAAGTGCTCAAAGATGTTTTTGGTTCATAG

Protein sequence

MSMATTSSLLHFSTIPIPIFTKLPKYHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTSKCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDHPPDTAHHLQQVLKDVFGS*
BLAST of Csa1G614650 vs. Swiss-Prot
Match: PLA17_ARATH (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana GN=At1g51440 PE=1 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 2.8e-184
Identity = 331/536 (61.75%), Postives = 390/536 (72.76%), Query Frame = 1

Query: 13  STIPIPIFTKLPKYHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTSKCSSSLPSLTTM 72
           +++ +PI  K P++        +++  ++F T+P   +    F   +  CSSS  S+++ 
Sbjct: 2   ASLSLPITLKNPRF-------FSSSPQNIFKTQPQTLVLTTKFKTCSIICSSSCTSISSS 61

Query: 73  EDQEDE--------DQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLHLRKE 132
             Q+ +        D   +E    E       L EIWR++QG NNWE  LDP+N HLR+E
Sbjct: 62  TTQQKQSNKQTHVSDNKREEKAEEEEEEKEVSLREIWREVQGCNNWEGQLDPMNNHLRRE 121

Query: 133 IIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKL-LMPDPGYNITRYLYATSNINL 192
           IIRYGEF+QACYDSFDFDPHSKYCG+CKY  S FF  L L    GY ITRYLYATSNINL
Sbjct: 122 IIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFFLNLDLHLHKGYTITRYLYATSNINL 181

Query: 193 PNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTVTYL 252
           PNFF+KSK +          +WSQHANWMG+VAVATD +E+ RLGRRDIVIAWRGTVTYL
Sbjct: 182 PNFFQKSKLSS---------IWSQHANWMGFVAVATDEEEVSRLGRRDIVIAWRGTVTYL 241

Query: 253 EWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRLVEK 312
           EWI+DLKDIL  A F  DD S+KIE GF+DLYT KE++C +  FSAREQ+LAEVKRL+E 
Sbjct: 242 EWIYDLKDILCSANF-GDDPSIKIELGFHDLYTKKEDSCKFSSFSAREQVLAEVKRLIEY 301

Query: 313 Y-CKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAGPR 372
           Y  +  G + SITVTGHSLGA+LAL++AYDIAE+ LN V         +PITV+SF+GPR
Sbjct: 302 YGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPENNYK---IPITVFSFSGPR 361

Query: 373 VGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHVGT 432
           VGNL+FKERC+ELGVKVLRV+NVHDKVP VPGI  NEK QFQKY+EE  +FPWS AHVG 
Sbjct: 362 VGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYVEEKTSFPWSYAHVGV 421

Query: 433 ELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKG----RKFRLETKRDIALVNKSC 492
           ELALDH  SPFL  T DLGCAHNLEA LHL+DGYHGK     ++F L TKRDIALVNKSC
Sbjct: 422 ELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKRFCLVTKRDIALVNKSC 481

Query: 493 DFLRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDH-PPDTAHHLQQVLKDV 534
           DFLR EY VPPCWRQDENKGMV+N +G+WV+PDRP LE H P D AHHLQQVL  V
Sbjct: 482 DFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGPEDIAHHLQQVLGKV 517

BLAST of Csa1G614650 vs. Swiss-Prot
Match: PLA15_ARATH (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana GN=At1g06800 PE=1 SV=2)

HSP 1 Score: 504.2 bits (1297), Expect = 1.7e-141
Identity = 260/473 (54.97%), Postives = 324/473 (68.50%), Query Frame = 1

Query: 69  LTTMEDQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLHLRKEIIRY 128
           LTT ED    D +  E+           L + WR IQG ++W  L+DP++  LR E+IRY
Sbjct: 66  LTTAEDIRRRDGEAKES---------KRLRDTWRKIQGEDDWAGLMDPMDPVLRSELIRY 125

Query: 129 GEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSNINLPNFFK 188
           GE +QACYD+FDFDP S+YCG+C++   H F+ L + D GY + RYLYATSNINLPNFF 
Sbjct: 126 GEMAQACYDAFDFDPFSRYCGSCRFTRRHLFDSLGIIDSGYEVARYLYATSNINLPNFFS 185

Query: 189 KSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIK-RLGRRDIVIAWRGTVTYLEWIH 248
           KS++++         VWS++ANWMGYVAV+ D +  + RLGRRDI IAWRGTVT LEWI 
Sbjct: 186 KSRWSK---------VWSKNANWMGYVAVSDDNEATRCRLGRRDIAIAWRGTVTRLEWIA 245

Query: 249 DLKDILRPA---GFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRLVEKY 308
           DLKD L+P    GF   D +VK ESGF DLYT K+ +CN+  FSAREQ+L EVKRLVE+Y
Sbjct: 246 DLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFSKFSAREQVLTEVKRLVERY 305

Query: 309 CKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAGPRVG 368
               GEE+SITVTGHSLG ALA+++AYD+AEM +N   +G      +P+T +++ GPRVG
Sbjct: 306 GDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGK----VIPVTAFTYGGPRVG 365

Query: 369 NLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHVGTEL 428
           N++FKER E+LGVKVLRV+N HD V   PG+  NE+   Q  ++ A   PW  +HVG  L
Sbjct: 366 NIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAP-QALMKLAGGLPWCYSHVGEML 425

Query: 429 ALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDFLRKE 488
            LDH  SPFL  T DL  AHNLEA LHL+DGYHGKG++F L + RD ALVNK+ DFL+  
Sbjct: 426 PLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSSGRDPALVNKASDFLKDH 485

Query: 489 YGVPPCWRQDENKGMVRNNEGRWVVPDRPRLED-HPPD------TAHHLQQVL 531
           + VPP WRQD NKGMVRN +GRW+ PDR R +D H PD        HH  Q+L
Sbjct: 486 FMVPPYWRQDANKGMVRNTDGRWIQPDRIRADDQHAPDIHQLLTQLHHPSQLL 515

BLAST of Csa1G614650 vs. Swiss-Prot
Match: PLA16_ARATH (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana GN=At2g30550 PE=1 SV=2)

HSP 1 Score: 502.3 bits (1292), Expect = 6.6e-141
Identity = 252/440 (57.27%), Postives = 311/440 (70.68%), Query Frame = 1

Query: 97  LSEIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQAS 156
           L + WR IQG ++W  L+DP++  LR E+IRYGE +QACYD+FDFDP SKYCGT ++   
Sbjct: 101 LRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRL 160

Query: 157 HFFNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVA 216
            FF+ L M D GY + RYLYATSNINLPNFF KS++++         VWS++ANWMGYVA
Sbjct: 161 EFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSK---------VWSKNANWMGYVA 220

Query: 217 VATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAG---FIPDDTSVKIESGFYD 276
           V+ D     RLGRRDI IAWRGTVT LEWI DLKD L+P         D +VK+ESGF D
Sbjct: 221 VSDDETSRNRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLD 280

Query: 277 LYTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYDI 336
           LYT K+  C +  FSAREQIL EVKRLVE++      ++SITVTGHSLG ALA+++AYDI
Sbjct: 281 LYTDKDTTCKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDI 340

Query: 337 AEMKLNIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCEELGVKVLRVINVHDKVPMVP 396
           AEM+LN   +G      +P+TV ++ GPRVGN++F+ER EELGVKV+RV+NVHD VP  P
Sbjct: 341 AEMRLNRSKKGK----VIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSP 400

Query: 397 GIIANEKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSPFLMSTSDLGCAHNLEAYLHLI 456
           G+  NE       ++ A   PW  +HVG ELALDH +SPFL  + D+  AHNLEA LHL+
Sbjct: 401 GLFLNESRP-HALMKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL 460

Query: 457 DGYHGKGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRP 516
           DGYHGKG +F L + RD ALVNK+ DFL++   +PP WRQD NKGMVRN+EGRW+  +R 
Sbjct: 461 DGYHGKGERFVLSSGRDHALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERL 520

Query: 517 RLED-HPPDTAHHLQQVLKD 533
           R ED H PD  HHL Q+  D
Sbjct: 521 RFEDHHSPDIHHHLSQLRLD 526

BLAST of Csa1G614650 vs. Swiss-Prot
Match: PLA12_ARATH (Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana GN=DGL PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.6e-78
Identity = 180/442 (40.72%), Postives = 252/442 (57.01%), Query Frame = 1

Query: 21  TKLPKYHLVHTNMMNNNNNSLFSTKPTFS--IHYFNFTLPTSKCSSSLPSLTTMEDQEDE 80
           T   K++L H ++  + +  L  +  T S  I     +LP+S  S ++P           
Sbjct: 21  TPQQKHNLDHFSISQHTSKRLVVSSSTMSPPISSSPLSLPSSSSSQAIPPSRA------- 80

Query: 81  DQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDS 140
                 A+T+       PLS +WR+IQG NNWE L++PL+  L++EI RYG    A Y  
Sbjct: 81  -----PAVTL-------PLSRVWREIQGSNNWENLIEPLSPILQQEITRYGNLLSASYKG 140

Query: 141 FDFDPHSKYCGTCKYQASHFFNKLLMPDP-GYNITRYLYATSNINLPNFFKKSKFTRDES 200
           FD +P+SK   +CKY   +   +  + DP GY +T+Y+YAT +INL           +  
Sbjct: 141 FDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYIYATPDINL-----------NPI 200

Query: 201 RDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAG 260
           ++  NR     A W+GYVAV++D + +KRLGRRDI++ +RGTVT  EW+ +LK  L PA 
Sbjct: 201 KNEPNR-----ARWIGYVAVSSD-ESVKRLGRRDILVTFRGTVTNHEWLANLKSSLTPAR 260

Query: 261 FIPDDT--SVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISIT 320
             P +    VK+ESGF  LYT  E    +   S REQ+L+E+ RL+ K+    GEEISIT
Sbjct: 261 LDPHNPRPDVKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKH---KGEEISIT 320

Query: 321 VTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCEEL 380
           + GHS+G++LA + AYDIAE+ +N           VP+TV+SFAGPRVGNL FK+RCEEL
Sbjct: 321 LAGHSMGSSLAQLLAYDIAELGMN----QRRDEKPVPVTVFSFAGPRVGNLGFKKRCEEL 380

Query: 381 GVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCA---HVGTELALDHTHSP 440
           GVKVLR+ NV+D +  +PG + NE     + L      PWSC+   HVG EL LD     
Sbjct: 381 GVKVLRITNVNDPITKLPGFLFNENF---RSLGGVYELPWSCSCYTHVGVELTLD----- 410

Query: 441 FLMSTSDLGCAHNLEAYLHLID 455
                 ++ C H+LE Y+ L++
Sbjct: 441 -FFDVQNISCVHDLETYITLVN 410

BLAST of Csa1G614650 vs. Swiss-Prot
Match: PLA13_ARATH (Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana GN=At2g31690 PE=1 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.1e-78
Identity = 167/393 (42.49%), Postives = 232/393 (59.03%), Query Frame = 1

Query: 96  PLSEIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQA 155
           PLS +WR+IQG NNW+ L++PLN  L++EI RYG     CY +FD DP+SK    CKY  
Sbjct: 87  PLSRVWREIQGCNNWKDLIEPLNPLLQQEITRYGNLVSTCYKAFDLDPNSKRYLNCKYGK 146

Query: 156 SHFFNKLLMPDP-GYNITRYLYATSNINLPNFFKKSKFTRDESRDFSNRVWSQHANWMGY 215
                +  +  P  Y +T+Y+YAT +IN+     +++  R              A W+GY
Sbjct: 147 QTLLKETEIDQPEDYQVTKYIYATPDININISPIQNEMNR-------------RARWVGY 206

Query: 216 VAVATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAGFIPDDT--SVKIESGFY 275
           VA ++D D +KRLGRRDIV+ +RGTVT  EW+ +    L PA F P +    VK+ESGF 
Sbjct: 207 VAASSD-DSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHPHNPRLDVKVESGFL 266

Query: 276 DLYTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYD 335
            LYT  E    +   S R+Q+L+E+ RL+ KY    GEE+SIT+ GHS+G++LA + AYD
Sbjct: 267 SLYTSDESESKFGLESCRQQLLSEISRLMNKY---KGEEMSITLAGHSMGSSLAQLLAYD 326

Query: 336 IAEMKLNIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCEELGVKVLRVINVHDKVPMV 395
           IAE+ LN     G     +P+TV+SFAGPRVGNL+FK+RCEELGVKVLR+ NV+D V  +
Sbjct: 327 IAELGLNRRIGKGD----IPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVNDPVTKL 386

Query: 396 PGIIANEKLQFQKYLEEAIAFPWSCA---HVGTELALDHTHSPFLMSTSDLGCAHNLEAY 455
           PG++ NE     + L      PWSC+   HVG EL LD           ++ C H+L+ Y
Sbjct: 387 PGVLFNENF---RVLGGFYELPWSCSCYVHVGVELTLD------FFDVQNISCVHDLQTY 446

Query: 456 LHLIDGYHGKGRKFRLETKRDIALVNKSCDFLR 483
           + L++      R   +++  D    N + +FL+
Sbjct: 447 IDLLNQRRTNSRS--VDSDEDEDSDNVALEFLK 447

BLAST of Csa1G614650 vs. TrEMBL
Match: A0A0A0LXE2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G614650 PE=4 SV=1)

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 536/536 (100.00%), Postives = 536/536 (100.00%), Query Frame = 1

Query: 1   MSMATTSSLLHFSTIPIPIFTKLPKYHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTS 60
           MSMATTSSLLHFSTIPIPIFTKLPKYHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTS
Sbjct: 1   MSMATTSSLLHFSTIPIPIFTKLPKYHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTS 60

Query: 61  KCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLH 120
           KCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLH
Sbjct: 61  KCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLH 120

Query: 121 LRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSN 180
           LRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSN
Sbjct: 121 LRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSN 180

Query: 181 INLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTV 240
           INLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTV
Sbjct: 181 INLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTV 240

Query: 241 TYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRL 300
           TYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRL
Sbjct: 241 TYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRL 300

Query: 301 VEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAG 360
           VEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAG
Sbjct: 301 VEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAG 360

Query: 361 PRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHV 420
           PRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHV
Sbjct: 361 PRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHV 420

Query: 421 GTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDF 480
           GTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDF
Sbjct: 421 GTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDF 480

Query: 481 LRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDHPPDTAHHLQQVLKDVFGS 537
           LRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDHPPDTAHHLQQVLKDVFGS
Sbjct: 481 LRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDHPPDTAHHLQQVLKDVFGS 536

BLAST of Csa1G614650 vs. TrEMBL
Match: A0A067JRF6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_25940 PE=4 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 2.3e-196
Identity = 342/471 (72.61%), Postives = 384/471 (81.53%), Query Frame = 1

Query: 61  KCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLH 120
           KCSSS+  LT         Q DDE + V    +  PLS+IW++IQG N+WE LLDP+N H
Sbjct: 51  KCSSSVSRLTP--------QLDDETL-VYNQEDERPLSQIWKEIQGCNDWEGLLDPMNSH 110

Query: 121 LRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSN 180
           LRKEIIRYGEF+Q CYDSFDFDPHSKYCGTCKYQ +HFF+KL M   GY I+RYLYATSN
Sbjct: 111 LRKEIIRYGEFAQTCYDSFDFDPHSKYCGTCKYQGAHFFDKLDMQGHGYQISRYLYATSN 170

Query: 181 INLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTV 240
           INLPNFF+KSK +          VWS HANWMGYVAV+TD +EI+RLGRRDIVIAWRGTV
Sbjct: 171 INLPNFFQKSKLSS---------VWSIHANWMGYVAVSTDEEEIQRLGRRDIVIAWRGTV 230

Query: 241 TYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRL 300
           TYLEWI+DLKDIL  A F  +D S+KIE GFYDLYT KE +C YC FSAREQ+LAE+KRL
Sbjct: 231 TYLEWIYDLKDILCSANFT-NDPSIKIELGFYDLYTKKENSCKYCTFSAREQVLAEIKRL 290

Query: 301 VEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAG 360
           ++ Y    GEEISIT+TGHSLGAALA+++AYDIAEMK+N +         +PITVYSF+G
Sbjct: 291 LDYY---RGEEISITITGHSLGAALAILSAYDIAEMKVNYM----DDKTKIPITVYSFSG 350

Query: 361 PRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHV 420
           PRVGNLKFKERCEEL VKVLRVINVHDKVP VPGI ANEK QFQKY+EE ++FPWS AHV
Sbjct: 351 PRVGNLKFKERCEELDVKVLRVINVHDKVPKVPGIFANEKFQFQKYVEETMSFPWSYAHV 410

Query: 421 GTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDF 480
           G ELALDHTHSPFL  T+DLGCAHNLE +LHL+DGYHGKGRKF L TKRDIALVNKSCDF
Sbjct: 411 GVELALDHTHSPFLRPTNDLGCAHNLEVHLHLVDGYHGKGRKFVLATKRDIALVNKSCDF 470

Query: 481 LRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDHPPDTAHHLQQVLK 532
           LR EYGVPP WRQDENKGM+RN EGRWVVPDRPR+E H  DTAHHL+QVLK
Sbjct: 471 LRSEYGVPPHWRQDENKGMIRNREGRWVVPDRPRVEAHRADTAHHLEQVLK 495

BLAST of Csa1G614650 vs. TrEMBL
Match: F6GU34_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g02390 PE=4 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 1.5e-195
Identity = 344/490 (70.20%), Postives = 385/490 (78.57%), Query Frame = 1

Query: 39  NSLFSTKPTFSIHYFNFTLPTSKCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLS 98
           N  FS KPT  I          KCSS + SLT   D E            E      PL 
Sbjct: 38  NKPFSPKPTTLI----------KCSS-VSSLTPRLDHEPAR---------ELKQEDKPLR 97

Query: 99  EIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHF 158
           ++W +IQG NNWE LLDP+N +LRKEIIRYGEF+QACYDSFDFDPHSKYCGTCKYQ +HF
Sbjct: 98  DVWEEIQGCNNWEGLLDPMNPNLRKEIIRYGEFAQACYDSFDFDPHSKYCGTCKYQGAHF 157

Query: 159 FNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVA 218
           F KL M D GY I+RYLYATSNINLPNFF+KSK +          VWS HANWMGY+AV 
Sbjct: 158 FQKLDMADRGYQISRYLYATSNINLPNFFQKSKMSS---------VWSPHANWMGYIAVT 217

Query: 219 TDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMK 278
           TD  EIKRLGRRDI+IAWRGTVTYLEWIHDLKDIL PA F  DD ++KIESGFYDLYT K
Sbjct: 218 TDEKEIKRLGRRDIIIAWRGTVTYLEWIHDLKDILCPAHF-RDDPNIKIESGFYDLYTKK 277

Query: 279 EENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKL 338
           E NC +C FSAREQ+LAE+KRLVE+Y     EEISIT+TGHSLGAALAL++AYDIAEM L
Sbjct: 278 ENNCKFCSFSAREQVLAEIKRLVERY---KDEEISITITGHSLGAALALLSAYDIAEMNL 337

Query: 339 NIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIAN 398
           N+  +       +PI+V+SF+GPRVGNLKFKERC+ELGVKVLRVINVHDKVP VPGII N
Sbjct: 338 NVRNKS-----RIPISVFSFSGPRVGNLKFKERCDELGVKVLRVINVHDKVPTVPGIITN 397

Query: 399 EKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHG 458
           EK Q+QKY+EE I+FPWS AHVG ELALDHTHSPFL  T+DLGCAHNLEA+LHL+DGYHG
Sbjct: 398 EKFQYQKYIEETISFPWSYAHVGVELALDHTHSPFLKPTNDLGCAHNLEAHLHLVDGYHG 457

Query: 459 KGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDH 518
           K RKF L TKRDIALVNKSCDFLR EYGVP  WRQDENKGMVR ++GRWV+P+RPR+E H
Sbjct: 458 KDRKFSLATKRDIALVNKSCDFLRSEYGVPGNWRQDENKGMVRASDGRWVLPERPRMEAH 489

Query: 519 PPDTAHHLQQ 529
           PPDTAHHL+Q
Sbjct: 518 PPDTAHHLEQ 489

BLAST of Csa1G614650 vs. TrEMBL
Match: M5WGU9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004631mg PE=4 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 4.7e-194
Identity = 337/458 (73.58%), Postives = 378/458 (82.53%), Query Frame = 1

Query: 80  QDDDEAITVET----NNNFPPLSEIWRDIQGMNNWEALLDP-LNLHLRKEIIRYGEFSQA 139
           Q D E I  +T     +N  PL EIW++IQG+NNWE LLDP LN HLR+EIIRYGEF+QA
Sbjct: 45  QLDHENIEPQTLVFHEDNHRPLHEIWKEIQGLNNWEGLLDPTLNSHLRQEIIRYGEFAQA 104

Query: 140 CYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFTR 199
           CYDSFDFDPHSKYCGTCKYQ +HFF  L M D GY I+RYLYATSNINLPNFF+KSK   
Sbjct: 105 CYDSFDFDPHSKYCGTCKYQGAHFFENLDMADRGYQISRYLYATSNINLPNFFQKSKL-- 164

Query: 200 DESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILR 259
                    VWS+HANWMG+VAVATD D+IKRLGRRDIVIAWRGTVTYLEWI+DLKDIL 
Sbjct: 165 -------GSVWSRHANWMGFVAVATDPDQIKRLGRRDIVIAWRGTVTYLEWIYDLKDILH 224

Query: 260 PAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISI 319
           PA F  +D S+KIESGFYDLYT KE+ C +C FSAREQ+LAEVKRL E Y    GEEISI
Sbjct: 225 PAQF-RNDPSIKIESGFYDLYTKKEDECRFCSFSAREQLLAEVKRLRELY---QGEEISI 284

Query: 320 TVTGHSLGAALALITAYDIAEMKLNIVARGGSS--AVAVPITVYSFAGPRVGNLKFKERC 379
           T+TGHSLGAALA+++AYDIAEM LNIV  G     +  +PITVYSF+GPRVGNL+FKERC
Sbjct: 285 TITGHSLGAALAILSAYDIAEMGLNIVHDGHHELESTKIPITVYSFSGPRVGNLRFKERC 344

Query: 380 EELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSP 439
           +ELGVKVLRV+NVHDKVP VPGII NEK +FQKY+E+ IAFPWS AHVG  L LDH  SP
Sbjct: 345 DELGVKVLRVVNVHDKVPRVPGIITNEKFRFQKYIEDTIAFPWSYAHVGVALELDHNQSP 404

Query: 440 FLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWR 499
           FL  T+D GCAHNLEA+LHL+DGYHGKG+KF L TKRDIALVNKSCDFLR EYGVPP WR
Sbjct: 405 FLKPTNDFGCAHNLEAHLHLVDGYHGKGQKFCLVTKRDIALVNKSCDFLRAEYGVPPHWR 464

Query: 500 QDENKGMVRNNEGRWVVPDRPRLEDHPPDTAHHLQQVL 531
           QDENKGMVR+ +GRWV+P+RPR+E HPPDTAHHLQQVL
Sbjct: 465 QDENKGMVRSKDGRWVLPERPRVEAHPPDTAHHLQQVL 489

BLAST of Csa1G614650 vs. TrEMBL
Match: A0A061GZA9_THECC (Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao GN=TCM_040740 PE=4 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 5.2e-193
Identity = 338/493 (68.56%), Postives = 390/493 (79.11%), Query Frame = 1

Query: 39  NSLFSTKPTFSIHYFNFTLPTSKCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLS 98
           N +F  KP F          +S C     SL+++  Q +E      A+ +E +    PL 
Sbjct: 35  NFIFQYKPCFP--------SSSTCIKRSTSLSSLTPQLEE------ALVLEEDER--PLC 94

Query: 99  EIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHF 158
           +IW++IQG N+W+ LLDP+N HLR+EIIRYGEF+QACYDSFDFDPHSKYCG+CKYQ +HF
Sbjct: 95  QIWKEIQGCNDWDGLLDPMNPHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYQGAHF 154

Query: 159 FNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVA 218
           F KL M D GY I+RYLYATSNINLPNFF+KS  +          VWS HANWMGYVAV 
Sbjct: 155 FEKLGMADRGYQISRYLYATSNINLPNFFQKSNLSS---------VWSTHANWMGYVAVC 214

Query: 219 TDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMK 278
           TD DEIKRLGRRDIVI+WRGTVTYLEWI+DLKDIL  A F  D  S+K+E GFYDLYT K
Sbjct: 215 TDEDEIKRLGRRDIVISWRGTVTYLEWIYDLKDILHQANFTKDP-SIKMELGFYDLYTKK 274

Query: 279 EENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKL 338
           E  CNYC FSAREQ+LAE+KRL+E Y    GEEISIT+TGHSLGAALALITAYDIAE+ L
Sbjct: 275 ENACNYCSFSAREQVLAEIKRLLEYY---DGEEISITITGHSLGAALALITAYDIAELGL 334

Query: 339 NIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIAN 398
           N+V  G  S   VPITVYSFAGPRVGNLKFKERC+ELGVKVLR +NVHDKVP VPGI AN
Sbjct: 335 NLVEEGELSN-KVPITVYSFAGPRVGNLKFKERCDELGVKVLRAVNVHDKVPTVPGIFAN 394

Query: 399 EKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHG 458
           EKLQFQKYLEEA++FPWS AHVG ELALDHT SPFL ST+DL CAHNLEA+LHL+DGYHG
Sbjct: 395 EKLQFQKYLEEAVSFPWSYAHVGVELALDHTCSPFLKSTNDLACAHNLEAHLHLLDGYHG 454

Query: 459 KGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDH 518
           KGR+F L  KRDIALVNK  +FL+ +YGVPP WRQDENKGMVRN++GRWV+P+RPR+E H
Sbjct: 455 KGRRFCLANKRDIALVNKDSNFLKSDYGVPPYWRQDENKGMVRNSDGRWVLPERPRVEAH 497

Query: 519 PPDTAHHLQQVLK 532
           P D +HHL+++LK
Sbjct: 515 PHDISHHLEKILK 497

BLAST of Csa1G614650 vs. TAIR10
Match: AT1G51440.1 (AT1G51440.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 646.4 bits (1666), Expect = 1.6e-185
Identity = 331/536 (61.75%), Postives = 390/536 (72.76%), Query Frame = 1

Query: 13  STIPIPIFTKLPKYHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTSKCSSSLPSLTTM 72
           +++ +PI  K P++        +++  ++F T+P   +    F   +  CSSS  S+++ 
Sbjct: 2   ASLSLPITLKNPRF-------FSSSPQNIFKTQPQTLVLTTKFKTCSIICSSSCTSISSS 61

Query: 73  EDQEDE--------DQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLHLRKE 132
             Q+ +        D   +E    E       L EIWR++QG NNWE  LDP+N HLR+E
Sbjct: 62  TTQQKQSNKQTHVSDNKREEKAEEEEEEKEVSLREIWREVQGCNNWEGQLDPMNNHLRRE 121

Query: 133 IIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKL-LMPDPGYNITRYLYATSNINL 192
           IIRYGEF+QACYDSFDFDPHSKYCG+CKY  S FF  L L    GY ITRYLYATSNINL
Sbjct: 122 IIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFFLNLDLHLHKGYTITRYLYATSNINL 181

Query: 193 PNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTVTYL 252
           PNFF+KSK +          +WSQHANWMG+VAVATD +E+ RLGRRDIVIAWRGTVTYL
Sbjct: 182 PNFFQKSKLSS---------IWSQHANWMGFVAVATDEEEVSRLGRRDIVIAWRGTVTYL 241

Query: 253 EWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRLVEK 312
           EWI+DLKDIL  A F  DD S+KIE GF+DLYT KE++C +  FSAREQ+LAEVKRL+E 
Sbjct: 242 EWIYDLKDILCSANF-GDDPSIKIELGFHDLYTKKEDSCKFSSFSAREQVLAEVKRLIEY 301

Query: 313 Y-CKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAGPR 372
           Y  +  G + SITVTGHSLGA+LAL++AYDIAE+ LN V         +PITV+SF+GPR
Sbjct: 302 YGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPENNYK---IPITVFSFSGPR 361

Query: 373 VGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHVGT 432
           VGNL+FKERC+ELGVKVLRV+NVHDKVP VPGI  NEK QFQKY+EE  +FPWS AHVG 
Sbjct: 362 VGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYVEEKTSFPWSYAHVGV 421

Query: 433 ELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKG----RKFRLETKRDIALVNKSC 492
           ELALDH  SPFL  T DLGCAHNLEA LHL+DGYHGK     ++F L TKRDIALVNKSC
Sbjct: 422 ELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKRFCLVTKRDIALVNKSC 481

Query: 493 DFLRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDH-PPDTAHHLQQVLKDV 534
           DFLR EY VPPCWRQDENKGMV+N +G+WV+PDRP LE H P D AHHLQQVL  V
Sbjct: 482 DFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGPEDIAHHLQQVLGKV 517

BLAST of Csa1G614650 vs. TAIR10
Match: AT1G06800.1 (AT1G06800.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 504.2 bits (1297), Expect = 9.8e-143
Identity = 260/473 (54.97%), Postives = 324/473 (68.50%), Query Frame = 1

Query: 69  LTTMEDQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLHLRKEIIRY 128
           LTT ED    D +  E+           L + WR IQG ++W  L+DP++  LR E+IRY
Sbjct: 66  LTTAEDIRRRDGEAKES---------KRLRDTWRKIQGEDDWAGLMDPMDPVLRSELIRY 125

Query: 129 GEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSNINLPNFFK 188
           GE +QACYD+FDFDP S+YCG+C++   H F+ L + D GY + RYLYATSNINLPNFF 
Sbjct: 126 GEMAQACYDAFDFDPFSRYCGSCRFTRRHLFDSLGIIDSGYEVARYLYATSNINLPNFFS 185

Query: 189 KSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIK-RLGRRDIVIAWRGTVTYLEWIH 248
           KS++++         VWS++ANWMGYVAV+ D +  + RLGRRDI IAWRGTVT LEWI 
Sbjct: 186 KSRWSK---------VWSKNANWMGYVAVSDDNEATRCRLGRRDIAIAWRGTVTRLEWIA 245

Query: 249 DLKDILRPA---GFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRLVEKY 308
           DLKD L+P    GF   D +VK ESGF DLYT K+ +CN+  FSAREQ+L EVKRLVE+Y
Sbjct: 246 DLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFSKFSAREQVLTEVKRLVERY 305

Query: 309 CKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAGPRVG 368
               GEE+SITVTGHSLG ALA+++AYD+AEM +N   +G      +P+T +++ GPRVG
Sbjct: 306 GDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGK----VIPVTAFTYGGPRVG 365

Query: 369 NLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHVGTEL 428
           N++FKER E+LGVKVLRV+N HD V   PG+  NE+   Q  ++ A   PW  +HVG  L
Sbjct: 366 NIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAP-QALMKLAGGLPWCYSHVGEML 425

Query: 429 ALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDFLRKE 488
            LDH  SPFL  T DL  AHNLEA LHL+DGYHGKG++F L + RD ALVNK+ DFL+  
Sbjct: 426 PLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSSGRDPALVNKASDFLKDH 485

Query: 489 YGVPPCWRQDENKGMVRNNEGRWVVPDRPRLED-HPPD------TAHHLQQVL 531
           + VPP WRQD NKGMVRN +GRW+ PDR R +D H PD        HH  Q+L
Sbjct: 486 FMVPPYWRQDANKGMVRNTDGRWIQPDRIRADDQHAPDIHQLLTQLHHPSQLL 515

BLAST of Csa1G614650 vs. TAIR10
Match: AT2G30550.2 (AT2G30550.2 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 502.3 bits (1292), Expect = 3.7e-142
Identity = 252/440 (57.27%), Postives = 311/440 (70.68%), Query Frame = 1

Query: 97  LSEIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQAS 156
           L + WR IQG ++W  L+DP++  LR E+IRYGE +QACYD+FDFDP SKYCGT ++   
Sbjct: 101 LRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRL 160

Query: 157 HFFNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVA 216
            FF+ L M D GY + RYLYATSNINLPNFF KS++++         VWS++ANWMGYVA
Sbjct: 161 EFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSK---------VWSKNANWMGYVA 220

Query: 217 VATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAG---FIPDDTSVKIESGFYD 276
           V+ D     RLGRRDI IAWRGTVT LEWI DLKD L+P         D +VK+ESGF D
Sbjct: 221 VSDDETSRNRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLD 280

Query: 277 LYTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYDI 336
           LYT K+  C +  FSAREQIL EVKRLVE++      ++SITVTGHSLG ALA+++AYDI
Sbjct: 281 LYTDKDTTCKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDI 340

Query: 337 AEMKLNIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCEELGVKVLRVINVHDKVPMVP 396
           AEM+LN   +G      +P+TV ++ GPRVGN++F+ER EELGVKV+RV+NVHD VP  P
Sbjct: 341 AEMRLNRSKKGK----VIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSP 400

Query: 397 GIIANEKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSPFLMSTSDLGCAHNLEAYLHLI 456
           G+  NE       ++ A   PW  +HVG ELALDH +SPFL  + D+  AHNLEA LHL+
Sbjct: 401 GLFLNESRP-HALMKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL 460

Query: 457 DGYHGKGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRP 516
           DGYHGKG +F L + RD ALVNK+ DFL++   +PP WRQD NKGMVRN+EGRW+  +R 
Sbjct: 461 DGYHGKGERFVLSSGRDHALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERL 520

Query: 517 RLED-HPPDTAHHLQQVLKD 533
           R ED H PD  HHL Q+  D
Sbjct: 521 RFEDHHSPDIHHHLSQLRLD 526

BLAST of Csa1G614650 vs. TAIR10
Match: AT1G30370.1 (AT1G30370.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 357.5 bits (916), Expect = 1.5e-98
Identity = 193/419 (46.06%), Postives = 259/419 (61.81%), Query Frame = 1

Query: 97  LSEIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQAS 156
           +S++WR++ G NNWE LLDPL+  LR+E+ +YGEF ++ YDS DFDP S++CG+ +Y  +
Sbjct: 114 ISKMWRELHGSNNWENLLDPLHPWLRREVTKYGEFVESVYDSLDFDPLSEFCGSSRYNRN 173

Query: 157 HFFNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVA 216
             F +L +   GY +T+Y+YA S +++P +F  S              WS+ +NWMG+VA
Sbjct: 174 KLFEELGLTRHGYKVTKYIYAMSRVDVPQWFLSSAL---------GETWSKDSNWMGFVA 233

Query: 217 VATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRP--AGFIPDDTSVKIESGFYDL 276
           V+ D + + R+GRRDIV+AWRGTVT  EW  DL+  + P         T VK++SGF  +
Sbjct: 234 VSGDRESL-RIGRRDIVVAWRGTVTPTEWFMDLRTSMEPFDCEGKHGKTVVKVQSGFLSI 293

Query: 277 YTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYDIA 336
           Y  K E   Y   SA EQ + EVKRLV  + K  GEE+S+T+TGHSLG ALAL+ AY+ A
Sbjct: 294 YNSKSELTRYNKESASEQTMDEVKRLV-NFFKDRGEEVSLTITGHSLGGALALMNAYEAA 353

Query: 337 EMKLNIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCEELGVKVLRVINVHDKVPMVPG 396
               ++ A  G+      I+V SF  PRVGNL FKE+   LGVKVLRV+N  D VP +PG
Sbjct: 354 R---DVPALSGN------ISVISFGAPRVGNLAFKEKLNSLGVKVLRVVNKQDIVPKLPG 413

Query: 397 IIANEKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSPFLMSTSDLGCAHNLEAYLHLID 456
           I+ N+ L   K         W   HVGT+L LD   SP++   SDLG AHNLE YLH++D
Sbjct: 414 IVFNKVL--NKLNPITSRLNWVYRHVGTQLKLDVFSSPYVKRDSDLGRAHNLEVYLHVLD 473

Query: 457 GYHGKGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRNNE-GRWVVPDR 513
           G+H K   FR+  +RD+A VNKS D L     +P  W Q  +KG++ N + GRWV P R
Sbjct: 474 GFHRKKSGFRVNARRDVASVNKSTDMLLDHLRIPEFWYQVAHKGLILNKQTGRWVKPVR 510

BLAST of Csa1G614650 vs. TAIR10
Match: AT1G05800.1 (AT1G05800.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 295.0 bits (754), Expect = 9.1e-80
Identity = 180/442 (40.72%), Postives = 252/442 (57.01%), Query Frame = 1

Query: 21  TKLPKYHLVHTNMMNNNNNSLFSTKPTFS--IHYFNFTLPTSKCSSSLPSLTTMEDQEDE 80
           T   K++L H ++  + +  L  +  T S  I     +LP+S  S ++P           
Sbjct: 21  TPQQKHNLDHFSISQHTSKRLVVSSSTMSPPISSSPLSLPSSSSSQAIPPSRA------- 80

Query: 81  DQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDS 140
                 A+T+       PLS +WR+IQG NNWE L++PL+  L++EI RYG    A Y  
Sbjct: 81  -----PAVTL-------PLSRVWREIQGSNNWENLIEPLSPILQQEITRYGNLLSASYKG 140

Query: 141 FDFDPHSKYCGTCKYQASHFFNKLLMPDP-GYNITRYLYATSNINLPNFFKKSKFTRDES 200
           FD +P+SK   +CKY   +   +  + DP GY +T+Y+YAT +INL           +  
Sbjct: 141 FDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYIYATPDINL-----------NPI 200

Query: 201 RDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAG 260
           ++  NR     A W+GYVAV++D + +KRLGRRDI++ +RGTVT  EW+ +LK  L PA 
Sbjct: 201 KNEPNR-----ARWIGYVAVSSD-ESVKRLGRRDILVTFRGTVTNHEWLANLKSSLTPAR 260

Query: 261 FIPDDT--SVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISIT 320
             P +    VK+ESGF  LYT  E    +   S REQ+L+E+ RL+ K+    GEEISIT
Sbjct: 261 LDPHNPRPDVKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKH---KGEEISIT 320

Query: 321 VTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCEEL 380
           + GHS+G++LA + AYDIAE+ +N           VP+TV+SFAGPRVGNL FK+RCEEL
Sbjct: 321 LAGHSMGSSLAQLLAYDIAELGMN----QRRDEKPVPVTVFSFAGPRVGNLGFKKRCEEL 380

Query: 381 GVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCA---HVGTELALDHTHSP 440
           GVKVLR+ NV+D +  +PG + NE     + L      PWSC+   HVG EL LD     
Sbjct: 381 GVKVLRITNVNDPITKLPGFLFNENF---RSLGGVYELPWSCSCYTHVGVELTLD----- 410

Query: 441 FLMSTSDLGCAHNLEAYLHLID 455
                 ++ C H+LE Y+ L++
Sbjct: 441 -FFDVQNISCVHDLETYITLVN 410

BLAST of Csa1G614650 vs. NCBI nr
Match: gi|778663679|ref|XP_011660136.1| (PREDICTED: phospholipase A1-Igamma3, chloroplastic [Cucumis sativus])

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 536/536 (100.00%), Postives = 536/536 (100.00%), Query Frame = 1

Query: 1   MSMATTSSLLHFSTIPIPIFTKLPKYHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTS 60
           MSMATTSSLLHFSTIPIPIFTKLPKYHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTS
Sbjct: 1   MSMATTSSLLHFSTIPIPIFTKLPKYHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTS 60

Query: 61  KCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLH 120
           KCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLH
Sbjct: 61  KCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLH 120

Query: 121 LRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSN 180
           LRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSN
Sbjct: 121 LRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSN 180

Query: 181 INLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTV 240
           INLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTV
Sbjct: 181 INLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTV 240

Query: 241 TYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRL 300
           TYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRL
Sbjct: 241 TYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRL 300

Query: 301 VEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAG 360
           VEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAG
Sbjct: 301 VEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAG 360

Query: 361 PRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHV 420
           PRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHV
Sbjct: 361 PRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHV 420

Query: 421 GTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDF 480
           GTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDF
Sbjct: 421 GTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDF 480

Query: 481 LRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDHPPDTAHHLQQVLKDVFGS 537
           LRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDHPPDTAHHLQQVLKDVFGS
Sbjct: 481 LRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDHPPDTAHHLQQVLKDVFGS 536

BLAST of Csa1G614650 vs. NCBI nr
Match: gi|659099071|ref|XP_008450415.1| (PREDICTED: phospholipase A1-Igamma3, chloroplastic [Cucumis melo])

HSP 1 Score: 998.8 bits (2581), Expect = 3.6e-288
Identity = 482/505 (95.45%), Postives = 491/505 (97.23%), Query Frame = 1

Query: 33  MMNNNNNSLFSTKPTFSIHYFNFTLPTSKCSSSLPSLTTMEDQEDEDQDDDEAITVETNN 92
           M+NNNNNSLFST PT SIHYFNF LPTSKCSSSLPSLTTMEDQEDEDQDD EAIT+ETNN
Sbjct: 1   MINNNNNSLFSTNPTLSIHYFNFILPTSKCSSSLPSLTTMEDQEDEDQDDVEAITMETNN 60

Query: 93  NFPPLSEIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCK 152
           NF PLSEIWRDIQGMNNWE LLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCK
Sbjct: 61  NFAPLSEIWRDIQGMNNWEGLLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCK 120

Query: 153 YQASHFFNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFTRDESRDFSNRVWSQHANWM 212
           YQASHFFNKLLMPDPGYNITRYLYATSNINLPNFFKKS FTRDESR+FSNRVWSQHANWM
Sbjct: 121 YQASHFFNKLLMPDPGYNITRYLYATSNINLPNFFKKSNFTRDESRNFSNRVWSQHANWM 180

Query: 213 GYVAVATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAGFIPDDTSVKIESGFY 272
           GYVAVATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAGFIPDDTSVKIESGFY
Sbjct: 181 GYVAVATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAGFIPDDTSVKIESGFY 240

Query: 273 DLYTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYD 332
           DLYTMKEENCNY GFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYD
Sbjct: 241 DLYTMKEENCNYSGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYD 300

Query: 333 IAEMKLNIVARG-GSSAVAVPITVYSFAGPRVGNLKFKERCEELGVKVLRVINVHDKVPM 392
           IAEMKLNIVARG GSS V VPITVYSFAGPRVGNLKFKERCEELGVK LRVIN+HDKVPM
Sbjct: 301 IAEMKLNIVARGDGSSPVEVPITVYSFAGPRVGNLKFKERCEELGVKALRVINIHDKVPM 360

Query: 393 VPGIIANEKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSPFLMSTSDLGCAHNLEAYLH 452
           VPGIIANEKLQFQKYLEEAIAFPWS AHVGTELALDHTHSPFLMST+DLGCAHNLEA+LH
Sbjct: 361 VPGIIANEKLQFQKYLEEAIAFPWSYAHVGTELALDHTHSPFLMSTNDLGCAHNLEAHLH 420

Query: 453 LIDGYHGKGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRNNEGRWVVPD 512
           LIDGYHGKGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRN+EGRWVVPD
Sbjct: 421 LIDGYHGKGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRNSEGRWVVPD 480

Query: 513 RPRLEDHPPDTAHHLQQVLKDVFGS 537
           RPRL+DHP DTA+HLQQVLKDVFGS
Sbjct: 481 RPRLDDHPSDTANHLQQVLKDVFGS 505

BLAST of Csa1G614650 vs. NCBI nr
Match: gi|1009118165|ref|XP_015875715.1| (PREDICTED: phospholipase A1-Igamma3, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 706.8 bits (1823), Expect = 2.8e-200
Identity = 345/509 (67.78%), Postives = 402/509 (78.98%), Query Frame = 1

Query: 26  YHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTSKCSSSLPSLTTMEDQEDEDQDDD-E 85
           YH+    ++ ++     S K   SI   + +   ++  + L      +D +D D  DD E
Sbjct: 33  YHIKSKTLIVSDTKLFSSPKLKISIRCSSVSSDYAEAETILTPQFDQDDDDDHDHHDDPE 92

Query: 86  AITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDSFDFDPH 145
           ++    +    PLSEIW++IQG+NNWE LLDPLN HLRKEIIRYGEF+Q+CYDSFDFDPH
Sbjct: 93  SLLFHEDQR--PLSEIWKEIQGINNWEGLLDPLNSHLRKEIIRYGEFAQSCYDSFDFDPH 152

Query: 146 SKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFTRDESRDFSNRV 205
           SKYCGTCKYQ +HFF K+ M D GY I+RYLYATSNINLPNFF+KSK +          V
Sbjct: 153 SKYCGTCKYQGAHFFEKMDMADRGYQISRYLYATSNINLPNFFQKSKMSS---------V 212

Query: 206 WSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAGFIPDDTS 265
           WS HANWMGY+AVATD DEIKRLGRRDIVIAWRGTVTYLEWI+DLKDILR A F   D S
Sbjct: 213 WSTHANWMGYIAVATDEDEIKRLGRRDIVIAWRGTVTYLEWIYDLKDILRSANFRGRDDS 272

Query: 266 VKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAA 325
           +KIESGFYDLYT KE  C YC +SAREQ+LAE+KRL+E Y    GEEISIT+TGHSLGAA
Sbjct: 273 IKIESGFYDLYTKKEHACKYCSYSAREQVLAEIKRLIEYY---EGEEISITLTGHSLGAA 332

Query: 326 LALITAYDIAEMKLNIV--ARGGSSAVAVPITVYSFAGPRVGNLKFKERCEELGVKVLRV 385
           LA+++AYDIAEMKLNIV    G      +PITV+SF+GPRVGNL FKERC+EL VKVLRV
Sbjct: 333 LAIVSAYDIAEMKLNIVRDVAGEEIVRKIPITVFSFSGPRVGNLHFKERCDELDVKVLRV 392

Query: 386 INVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSPFLMSTSDLGC 445
           +NVHD VP VPGI+ANEK QFQKY+EEAI+FPWS AHVG ELALDHTHSPFL +T+DLGC
Sbjct: 393 VNVHDVVPTVPGILANEKFQFQKYIEEAISFPWSYAHVGIELALDHTHSPFLKATNDLGC 452

Query: 446 AHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRN 505
           AHNLE +LHL+DGY GKGR+FRL TKRDIALVNK+ D LR EYGVPP WRQDENKGMVRN
Sbjct: 453 AHNLEVHLHLVDGYQGKGRRFRLATKRDIALVNKNSDLLRNEYGVPPNWRQDENKGMVRN 512

Query: 506 NEGRWVVPDRPRLEDHPPDTAHHLQQVLK 532
           ++GRWV+P+RPR++ HPPD AHHL+QVLK
Sbjct: 513 SDGRWVLPERPRVDAHPPDMAHHLEQVLK 527

BLAST of Csa1G614650 vs. NCBI nr
Match: gi|720095644|ref|XP_010246745.1| (PREDICTED: phospholipase A1-Igamma3, chloroplastic-like [Nelumbo nucifera])

HSP 1 Score: 696.0 bits (1795), Expect = 5.0e-197
Identity = 349/518 (67.37%), Postives = 404/518 (77.99%), Query Frame = 1

Query: 14  TIPIPIFTKLPKYHLVHTNMMNNNNNSLFSTKPTFSIHYFNFTLPTSKCSSSLPSLTTME 73
           T P   F  LP ++L  T       N + S KP       +    TS   SS+ SLT++ 
Sbjct: 9   TKPKCFFPSLPGWNLSQTP------NLIISKKP------ISLKPRTSLKCSSVSSLTSIS 68

Query: 74  DQEDEDQDDDEAITVETNNNFPPLSEIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQ 133
           D+E   ++ ++ +  E  +    L EIW++IQG N+WE LLDP N  LRKEIIRYGEF+Q
Sbjct: 69  DEEIVVEEAEKLVMEEEKS----LREIWQEIQGCNDWEGLLDPTNPVLRKEIIRYGEFAQ 128

Query: 134 ACYDSFDFDPHSKYCGTCKYQASHFFNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFT 193
           ACYDSFDFDP SKYCGTCKYQ +HFF KL M + GY ++RY+YAT+NINLPNFF+K+K +
Sbjct: 129 ACYDSFDFDPRSKYCGTCKYQGAHFFQKLYMTNLGYQVSRYVYATTNINLPNFFQKAKIS 188

Query: 194 RDESRDFSNRVWSQHANWMGYVAVATDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDIL 253
                     VWS HANWMGYVAVATD  EIKRLGRRDI++AWRGTVTYLEWIHDLKDIL
Sbjct: 189 S---------VWSPHANWMGYVAVATDEQEIKRLGRRDILVAWRGTVTYLEWIHDLKDIL 248

Query: 254 RPAGFIPDDTSVKIESGFYDLYTMKEENCNYCGFSAREQILAEVKRLVEKYCKLGGEEIS 313
            PA F  DD S+KIESGF+DLYT KE  CNYC FSAREQILAE+KRLVE+Y    GEE+S
Sbjct: 249 HPARF-GDDPSIKIESGFFDLYTKKEHKCNYCSFSAREQILAEIKRLVERY---KGEELS 308

Query: 314 ITVTGHSLGAALALITAYDIAEMKLNIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCE 373
           IT+TGHSLGAALAL++AYDIAEMKLNI   G   A  +PITV+SFAGPRVGNLKFKERC+
Sbjct: 309 ITITGHSLGAALALLSAYDIAEMKLNIGDSGHIQA-RIPITVFSFAGPRVGNLKFKERCD 368

Query: 374 ELGVKVLRVINVHDKVPMVPGIIANEKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSPF 433
           ELGVKVLRVINVHDKVP VPG+IANEK QFQKY+EE ++FPWS AHVG ELALDHTHSPF
Sbjct: 369 ELGVKVLRVINVHDKVPTVPGVIANEKFQFQKYMEEVMSFPWSYAHVGLELALDHTHSPF 428

Query: 434 LMSTSDLGCAHNLEAYLHLIDGYHGKGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQ 493
           L  T DL CAHNLE +LHL+DGYHGK R+F L TKRDIALVNKSCDFLR +YGVPP WRQ
Sbjct: 429 LKPTFDLFCAHNLEVHLHLVDGYHGKERRFCLATKRDIALVNKSCDFLRVDYGVPPFWRQ 488

Query: 494 DENKGMVRNNEGRWVVPDRPRLEDHPPDTAHHLQQVLK 532
           DENKGMVR  +GRW++P+RPR+E HPPDTAHH++QVLK
Sbjct: 489 DENKGMVRTTDGRWILPERPRMEAHPPDTAHHVEQVLK 496

BLAST of Csa1G614650 vs. NCBI nr
Match: gi|225435393|ref|XP_002285367.1| (PREDICTED: phospholipase A1-Igamma3, chloroplastic [Vitis vinifera])

HSP 1 Score: 695.7 bits (1794), Expect = 6.5e-197
Identity = 347/493 (70.39%), Postives = 388/493 (78.70%), Query Frame = 1

Query: 39  NSLFSTKPTFSIHYFNFTLPTSKCSSSLPSLTTMEDQEDEDQDDDEAITVETNNNFPPLS 98
           N  FS KPT  I          KCSS + SLT   D E            E      PL 
Sbjct: 38  NKPFSPKPTTLI----------KCSS-VSSLTPRLDHEPAR---------ELKQEDKPLR 97

Query: 99  EIWRDIQGMNNWEALLDPLNLHLRKEIIRYGEFSQACYDSFDFDPHSKYCGTCKYQASHF 158
           ++W +IQG NNWE LLDP+N +LRKEIIRYGEF+QACYDSFDFDPHSKYCGTCKYQ +HF
Sbjct: 98  DVWEEIQGCNNWEGLLDPMNPNLRKEIIRYGEFAQACYDSFDFDPHSKYCGTCKYQGAHF 157

Query: 159 FNKLLMPDPGYNITRYLYATSNINLPNFFKKSKFTRDESRDFSNRVWSQHANWMGYVAVA 218
           F KL M D GY I+RYLYATSNINLPNFF+KSK +          VWS HANWMGY+AV 
Sbjct: 158 FQKLDMADRGYQISRYLYATSNINLPNFFQKSKMSS---------VWSPHANWMGYIAVT 217

Query: 219 TDADEIKRLGRRDIVIAWRGTVTYLEWIHDLKDILRPAGFIPDDTSVKIESGFYDLYTMK 278
           TD  EIKRLGRRDI+IAWRGTVTYLEWIHDLKDIL PA F  DD ++KIESGFYDLYT K
Sbjct: 218 TDEKEIKRLGRRDIIIAWRGTVTYLEWIHDLKDILCPAHF-RDDPNIKIESGFYDLYTKK 277

Query: 279 EENCNYCGFSAREQILAEVKRLVEKYCKLGGEEISITVTGHSLGAALALITAYDIAEMKL 338
           E NC +C FSAREQ+LAE+KRLVE+Y     EEISIT+TGHSLGAALAL++AYDIAEM L
Sbjct: 278 ENNCKFCSFSAREQVLAEIKRLVERY---KDEEISITITGHSLGAALALLSAYDIAEMNL 337

Query: 339 NIVARGGSSAVAVPITVYSFAGPRVGNLKFKERCEELGVKVLRVINVHDKVPMVPGIIAN 398
           N+  +       +PI+V+SF+GPRVGNLKFKERC+ELGVKVLRVINVHDKVP VPGII N
Sbjct: 338 NVRNKS-----RIPISVFSFSGPRVGNLKFKERCDELGVKVLRVINVHDKVPTVPGIITN 397

Query: 399 EKLQFQKYLEEAIAFPWSCAHVGTELALDHTHSPFLMSTSDLGCAHNLEAYLHLIDGYHG 458
           EK Q+QKY+EE I+FPWS AHVG ELALDHTHSPFL  T+DLGCAHNLEA+LHL+DGYHG
Sbjct: 398 EKFQYQKYIEETISFPWSYAHVGVELALDHTHSPFLKPTNDLGCAHNLEAHLHLVDGYHG 457

Query: 459 KGRKFRLETKRDIALVNKSCDFLRKEYGVPPCWRQDENKGMVRNNEGRWVVPDRPRLEDH 518
           K RKF L TKRDIALVNKSCDFLR EYGVP  WRQDENKGMVR ++GRWV+P+RPR+E H
Sbjct: 458 KDRKFSLATKRDIALVNKSCDFLRSEYGVPGNWRQDENKGMVRASDGRWVLPERPRMEAH 492

Query: 519 PPDTAHHLQQVLK 532
           PPDTAHHL+QVLK
Sbjct: 518 PPDTAHHLEQVLK 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PLA17_ARATH2.8e-18461.75Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana GN=At1g51440 PE=... [more]
PLA15_ARATH1.7e-14154.97Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana GN=At1g06800 PE=... [more]
PLA16_ARATH6.6e-14157.27Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana GN=At2g30550 PE=... [more]
PLA12_ARATH1.6e-7840.72Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana GN=DGL PE=1 SV=1[more]
PLA13_ARATH2.1e-7842.49Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana GN=At2g31690 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0LXE2_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G614650 PE=4 SV=1[more]
A0A067JRF6_JATCU2.3e-19672.61Uncharacterized protein OS=Jatropha curcas GN=JCGZ_25940 PE=4 SV=1[more]
F6GU34_VITVI1.5e-19570.20Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g02390 PE=4 SV=... [more]
M5WGU9_PRUPE4.7e-19473.58Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004631mg PE=4 SV=1[more]
A0A061GZA9_THECC5.2e-19368.56Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao GN=TCM_040740 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G51440.11.6e-18561.75 alpha/beta-Hydrolases superfamily protein[more]
AT1G06800.19.8e-14354.97 alpha/beta-Hydrolases superfamily protein[more]
AT2G30550.23.7e-14257.27 alpha/beta-Hydrolases superfamily protein[more]
AT1G30370.11.5e-9846.06 alpha/beta-Hydrolases superfamily protein[more]
AT1G05800.19.1e-8040.72 alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778663679|ref|XP_011660136.1|0.0e+00100.00PREDICTED: phospholipase A1-Igamma3, chloroplastic [Cucumis sativus][more]
gi|659099071|ref|XP_008450415.1|3.6e-28895.45PREDICTED: phospholipase A1-Igamma3, chloroplastic [Cucumis melo][more]
gi|1009118165|ref|XP_015875715.1|2.8e-20067.78PREDICTED: phospholipase A1-Igamma3, chloroplastic [Ziziphus jujuba][more]
gi|720095644|ref|XP_010246745.1|5.0e-19767.37PREDICTED: phospholipase A1-Igamma3, chloroplastic-like [Nelumbo nucifera][more]
gi|225435393|ref|XP_002285367.1|6.5e-19770.39PREDICTED: phospholipase A1-Igamma3, chloroplastic [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002921Fungal_lipase-like
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046486 glycerolipid metabolic process
biological_process GO:0009395 phospholipid catabolic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0005575 cellular_component
molecular_function GO:0047714 galactolipase activity
molecular_function GO:0008970 phosphatidylcholine 1-acylhydrolase activity
molecular_function GO:0004806 triglyceride lipase activity
molecular_function GO:0052740 1-acyl-2-lysophosphatidylserine acylhydrolase activity
molecular_function GO:0052739 phosphatidylserine 1-acylhydrolase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU131026cucumber EST collection version 3.0transcribed_cluster
CU136520cucumber EST collection version 3.0transcribed_cluster
CU141802cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa1G614650.1Csa1G614650.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU131026CU131026transcribed_cluster
CU136520CU136520transcribed_cluster
CU141802CU141802transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 233..394
score: 1.1
NoneNo IPR availablePANTHERPTHR31403FAMILY NOT NAMEDcoord: 59..528
score:
NoneNo IPR availablePANTHERPTHR31403:SF6PHOSPHOLIPASE A1-IGAMMA3, CHLOROPLASTICcoord: 59..528
score:

The following gene(s) are paralogous to this gene:

None