Csa1G570120 (gene) Cucumber (Chinese Long) v2

NameCsa1G570120
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A; contains IPR000120 (Amidase), IPR023631 (Amidase signature domain)
LocationChr1 : 21074062 .. 21078295 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTGGATGATTATGGAGCTTTCATAGAGAAATTTCTCCTGCAGATGAGCTCTCCTTCCGATAAACTTCCCTTAAGTGGACTCACTTTCGCTGTTAAAGACATGTGAGTTTCTGATTTTTTACTGTTTAAGTTCATCAAGTCTTTGGTGATCAATAGTCTGTGGAATCTGAAATATCTGTATAGAATCAAAAGATTTCAAGAACAGTTTTGAAATATTGTTACTGTTATATTTGTAGATATTGATGTTTCCATTTTTACAAGCCATCAACTGTTGGTTTTTCTATTTCAATGGAGCTTAGTTCGATAAAATAATTCTTGGTTCTTATGTCTTGAAATGACAACATGGCAGTTTTTTTTTTTCTACATGAAATTGGAGTATTGAAGTCAAACTTCTTTTGGACTTTAAACCAAACAGCTTTGATATAGAGGGGTATGTGGCTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACGGCACCAGCTGTGTTGACTATCCTAAGAGGAGGGGCCACTTGCATTGGCAAGACTATCATGGATGAAATGGCCTTTAGGTAACAAGAAAACTTACCCTGTACTTTATTTTTTTGTCTAGTTAGCTTTCATTTTTATATCGAGTATGAAAATATCGTGGGAATGAAGATTGAACTTTCAACCTTTTTAGAATGAGGGATTGAACCTTCAACTTTTAGGGGATGAAATGATGGAATGATGGAATGTGTATATTGCAATTGTGGGGTTGGGATTAATCCTTGGACCTCTAGGAAAAAGAATGTATGTCAATTGCGAGAGAGTTAAAGCTTCTATAGACACACACCTCATAACCTTTGCGATTCCTTAGTTGGTTTAGCTATATTATTATCATTCTTTTTCAAGCAGACAATTCTGCCTTCAAATTGGTTAAGACATGACATCAAGGTGAACTTGTGAGGTTTCTAATTTGTAATTGAAATGTTTGACCAATTGGTTCGTTCCAATTCCCAACATACACAGGCTTTTGAATGAATCTTAATCATATTTTTGGCAGTATAAATGGGGAAAACTTTCATTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCCAGTGGTTCTGGTGTTGCTGTTGCTGCAAAGCTTGTGGATTTCTCCTTAGGCGTGATTTTATTTCTAAGTCCTTGAAAAACTTGTGATTCCATGCCTTGAACTATTGTAAAAGACCATAACTCAATTTGTAGGAACTGATACCGGAGGTAGTGTAAGAGTGCCTGCATCCTACTGTGGAATTCTCGGATTCCGGCCTTCACATGGTGTGGTCTCTACGTCTGGAGTAATACCTATGACACAGAGCTTTGATACAGTAGGTAATGTGTAATCTCTCATTGTCACATTGTCTTTTGTAGTAGATAAGCACAATAGGCTGATTTTTTCTCTGAATTTTAAGTCGACTCTTGATCTCCTTTGTGAAACTGTCTATTTTTTTCAACTAAGGCCCTTTGGTGCTGTCATGCAAAGTTGTTTCCTTTTTTCTTTTTTAACTTTTACTTTTTAATTTTATAGGTAAAAAGGTTTGTTTGACAAACATTTGTGTTTTTTTTTTTAAAAAAAGAACCTAAAGTTTGGAAATACAGTACTCCATAATTTCCATTAAAATCATGACATCATTTTGATGATAATGTCTATGTTATTCATGATTTTGCATATAGATGAGCAAAGTGATGTAGATTCATAGCAGAGCCATTGGCGTCTCCTCTTTCCTTCAATACCAACTATATTTCGCTAGCTATCCTGCCATGTGAAAATGGTGTAGATTCCGCTAACACCATCTTCTCGTTTTAGGTTGGTTTGCCAGAGATTCCGCCATATTAAAGAGAGTAGGTTGGCTGCTGCTGCAAGAACCAGAGATTGAGCATTACAAGCCTACGAAAGTGTTCATTGCAGAAGATTGTTTCAAGCTCCTCTCGAACATTACTAGTGAACGATTGACACAAGCTTTTGTTAGCTCAGTGGAGAAGTTATTTGGTGGTAAGTTTAAGCTGACATTCTTGTATTAGTGATTTTGTGTTTTTTAAATTAGGTACCCTTTCATTTAGGAAGAATAAAGAAAGAGATCAAAGGCATACAAAAATTAGTTCACAATATGAATTCCAATCAAGCATAATACGACCTAAAGTGTAATTACAAAATCTTTTTTAACACCAAGGCTAGAAGAGAGGTATCAAATCTAACAAGGGACTAAAAACTTCTCCCCAAGAACTTGCAAACCTCTTAACATCAAGCCAATGAATCCATAAAACTGCTCAATAATGGGGTCTAGATTTTTTTAAAAAAATAATGTGTTATGATGTTTGTTTCTGTGCTTCTTAGATAACTAATATACTGGATGGAAGAAAAATTTGAAAACTACTTAGAATTGGTGCATTTGATCCCTGAGTTTTCAAATACACCTTTTTAGTCTCCAAATAGTTTTAAGAGGCCCTTGAAGTATTTTTTTTATTTCTATAATAATTATATGACATTTTGATTTACGAAATAAGTTTAAAAAATAATTTTAGGGAGAAGATGTAGTTTTAAAAAAATCATTTTTCAAACTTAAAAGAACTAAGAAACAGGCACTTTCTAAACCTCTATTTAAAACTTCATGGACTAAAAAGGTGTATAAAACTTAGGGACCAAACACATATACTCTAAAGAACTTGAGGACTTAAAAATGTATGTTTGGAAACTCAGAGACTAAACACACGATTCTTCAAAACTTTGGACTAAAAAGGAAAAATTTTCAAAAAGATTACTAATTTGATATTGGAGCTCATCTTTTAGATATACATTTAGGCGATTACTTATTTGACTCTTCAACTGCAGGCCATCTTATTAAACAAATTAGCCTTGGAAAGTATGTCGAGGATAAAGTTCCGAGCTTGAAGCATTTCATGATTGAAGAAAATGATGGCTATAAGCATAGCATACCATCCTTGGCAGCCCTTGTAAGATCTAAGCGATTTCTTCAGAGGTTTTGATCTTGTGATGAACCACAACTCCAATTCTGTCGAGCAAATTGACTCTGATATATAATTGTGTTGAATGAATGAATGATCTTTGCCCATGTTTTTGCTGCCCGCTTGCAGGCATGAATTCAACATCAATCATGGAGAATGGGTTCGGTCCTACCATTCTCATTTGGGTCCAGGAATATCAGAACGAATATTAGAATTCATCAGGGAGGTAACAGATGAAAATATTGATCTGAGTCGCTCCATTCAAATCGAACTGCGCGAAGCTCTTGCTGCTCTTCTTGAAGTATTTCCTTTTCCCCACACACACAATTTACTAAAATATTAAACTAATATAATCAGTATCTTAAACATGTGTGCATTTTGATATGTACTCAACTTTCATTGCTTTTTTGTGTAGAAAGAAAAAAAGCTATAACCAATGTAGTTTCCATTTGATATTTCAACATAATTATAGTGTCACTGGTCATTTTATTACTGATTTGATAGTTGCTATTGGATCGTCTACTTCGGAATAACGCAATGTTTCTTTTAGAAAAAAGGTTTACATGTATGATTACATTGTGTCATCTAACAAACATATCAAATTTCTTGGAAATTTATTTCTAGAACTATAATAGGACATTGGTGTTTGTTTCCCGAGAAGATTTATTCTATTCATTCTATCTCAAAAATCTATTATTAGACATTTCACTATAGAAATAAGCAGTTTGTGAATTTTAAAAAAATCTTGAGTTGCTATTGTTTATCATTTCCTTTCAGGATTTTGGAGTTCTTGCAATTCCTACAGTCCCAGGCCCTCCTCCAAAACTAAACACAGACATATCAGAGCAATACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCACCATTGCTGCTGTCTCTGGAGTCTGCCAGGTTCTCTCTCTCTTGAAAGTCTTTTCTTTCAAGTACAAGTGAGGATGGGGGATTTGAACCATAGACCTCTTGATTCTTAGCACTTGTAGTTATTGGTTGACCTCTCTTACTTGAAAGTTAAAAAGCAACCATTTTGATGTATTTTTTTTCTCACCAACTAATCCGCAGGTTAGCGTACCTCTAGGCTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAAACATGGTGCAGATGGATTTTTGCTCAATCTTGTTGATAGTCTTTACAGCACTCTAAAAGAAGAAGTTGAGGCAATCTACTAAAA

mRNA sequence

ATGGCTGTGGATGATTATGGAGCTTTCATAGAGAAATTTCTCCTGCAGATGAGCTCTCCTTCCGATAAACTTCCCTTAAGTGGACTCACTTTCGCTGTTAAAGACATCTTTGATATAGAGGGGTATGTGGCTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACGGCACCAGCTGTGTTGACTATCCTAAGAGGAGGGGCCACTTGCATTGGCAAGACTATCATGGATGAAATGGCCTTTAGTATAAATGGGGAAAACTTTCATTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCCAGTGGTTCTGGTGTTGCTGTTGCTGCAAAGCTTGTGGATTTCTCCTTAGGAACTGATACCGGAGGTAGTGTAAGAGTGCCTGCATCCTACTGTGGAATTCTCGGATTCCGGCCTTCACATGGTGTGGTCTCTACGTCTGGAGTAATACCTATGACACAGAGCTTTGATACAGTAGGTTGGTTTGCCAGAGATTCCGCCATATTAAAGAGAGTAGGTTGGCTGCTGCTGCAAGAACCAGAGATTGAGCATTACAAGCCTACGAAAGTGTTCATTGCAGAAGATTGTTTCAAGCTCCTCTCGAACATTACTAGTGAACGATTGACACAAGCTTTTGTTAGCTCAGTGGAGAAGTTATTTGGTGGCCATCTTATTAAACAAATTAGCCTTGGAAAGTATGTCGAGGATAAAGTTCCGAGCTTGAAGCATTTCATGATTGAAGAAAATGATGGCTATAAGCATAGCATACCATCCTTGGCAGCCCTTGTAAGATCTAAGCGATTTCTTCAGAGGCATGAATTCAACATCAATCATGGAGAATGGGTTCGGTCCTACCATTCTCATTTGGGTCCAGGAATATCAGAACGAATATTAGAATTCATCAGGGAGGTAACAGATGAAAATATTGATCTGAGTCGCTCCATTCAAATCGAACTGCGCGAAGCTCTTGCTGCTCTTCTTGAAGATTTTGGAGTTCTTGCAATTCCTACAGTCCCAGGCCCTCCTCCAAAACTAAACACAGACATATCAGAGCAATACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCACCATTGCTGCTGTCTCTGGAGTCTGCCAGGTTAGCGTACCTCTAGGCTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAAACATGGTGCAGATGGATTTTTGCTCAATCTTGTTGATAGTCTTTACAGCACTCTAAAAGAAGAAGTTGAGGCAATCTACTAA

Coding sequence (CDS)

ATGGCTGTGGATGATTATGGAGCTTTCATAGAGAAATTTCTCCTGCAGATGAGCTCTCCTTCCGATAAACTTCCCTTAAGTGGACTCACTTTCGCTGTTAAAGACATCTTTGATATAGAGGGGTATGTGGCTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACGGCACCAGCTGTGTTGACTATCCTAAGAGGAGGGGCCACTTGCATTGGCAAGACTATCATGGATGAAATGGCCTTTAGTATAAATGGGGAAAACTTTCATTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCCAGTGGTTCTGGTGTTGCTGTTGCTGCAAAGCTTGTGGATTTCTCCTTAGGAACTGATACCGGAGGTAGTGTAAGAGTGCCTGCATCCTACTGTGGAATTCTCGGATTCCGGCCTTCACATGGTGTGGTCTCTACGTCTGGAGTAATACCTATGACACAGAGCTTTGATACAGTAGGTTGGTTTGCCAGAGATTCCGCCATATTAAAGAGAGTAGGTTGGCTGCTGCTGCAAGAACCAGAGATTGAGCATTACAAGCCTACGAAAGTGTTCATTGCAGAAGATTGTTTCAAGCTCCTCTCGAACATTACTAGTGAACGATTGACACAAGCTTTTGTTAGCTCAGTGGAGAAGTTATTTGGTGGCCATCTTATTAAACAAATTAGCCTTGGAAAGTATGTCGAGGATAAAGTTCCGAGCTTGAAGCATTTCATGATTGAAGAAAATGATGGCTATAAGCATAGCATACCATCCTTGGCAGCCCTTGTAAGATCTAAGCGATTTCTTCAGAGGCATGAATTCAACATCAATCATGGAGAATGGGTTCGGTCCTACCATTCTCATTTGGGTCCAGGAATATCAGAACGAATATTAGAATTCATCAGGGAGGTAACAGATGAAAATATTGATCTGAGTCGCTCCATTCAAATCGAACTGCGCGAAGCTCTTGCTGCTCTTCTTGAAGATTTTGGAGTTCTTGCAATTCCTACAGTCCCAGGCCCTCCTCCAAAACTAAACACAGACATATCAGAGCAATACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCACCATTGCTGCTGTCTCTGGAGTCTGCCAGGTTAGCGTACCTCTAGGCTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAAACATGGTGCAGATGGATTTTTGCTCAATCTTGTTGATAGTCTTTACAGCACTCTAAAAGAAGAAGTTGAGGCAATCTACTAA

Protein sequence

MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYSTLKEEVEAIY*
BLAST of Csa1G570120 vs. Swiss-Prot
Match: AMI1_ARATH (Amidase 1 OS=Arabidopsis thaliana GN=AMI1 PE=1 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 3.3e-143
Identity = 262/419 (62.53%), Postives = 317/419 (75.66%), Query Frame = 1

Query: 4   DDYGAFIEKFLLQMSSPSDKLP-LSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTA 63
           +D+GAFIEK  +  +S S   P L GLTFA+KDIFD+EG V GFGNP+WLRTH  A  TA
Sbjct: 5   NDFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTA 64

Query: 64  PAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAK 123
           P V ++L  GAT +G TIMDEMA+SINGEN HYGTP+NP A DR+PGGSSSGS VAVAA+
Sbjct: 65  PVVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAAR 124

Query: 124 LVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKR 183
           LVDFS+GTDTGGSVRVPASYCGI GFRPSHG VST G+ PM QSFDTVGWFARD+A LKR
Sbjct: 125 LVDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKR 184

Query: 184 VGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGH-LIKQISL 243
           VG +LLQ+  +   +P+++ IA+DCFKL S +  + L Q  V SVEK FGG+ ++K+++L
Sbjct: 185 VGCVLLQQHHLNPIEPSQLIIADDCFKLCS-VPHDLLVQPLVGSVEKSFGGNTVVKKVNL 244

Query: 244 GKYVEDKVPSLKHFMIEEN--DGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           G+Y+   VPSLKHFM  ++     +  IPSL AL  S R LQRHEF INHG W+ S    
Sbjct: 245 GEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPE 304

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTD 363
            GPGISERI E IR  +DE ID  RS++ EL  AL+ LL + GVL IPTVPGPPP L  +
Sbjct: 305 FGPGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQAN 364

Query: 364 ISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSL 419
           ++    FR++AFSLL+IA VSG CQVS+PLGL+  LPVS+SL+AK+G+DGFLL+LVDSL
Sbjct: 365 VAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of Csa1G570120 vs. Swiss-Prot
Match: TOC64_PEA (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum GN=TOC64 PE=1 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.9e-115
Identity = 212/428 (49.53%), Postives = 293/428 (68.46%), Query Frame = 1

Query: 4   DDYGAFIEKFLL----QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPAN 63
           +D+GAF+ K  L      + P    PLS L FA+ DIFDIEG+V+ FG+PEW RTH PA+
Sbjct: 35  EDFGAFLLKLKLLPPPPPAPPKAPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPAS 94

Query: 64  QTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAV 123
            TA AV  ++  GATCIG T++DE+A+ I+GEN H+GTP NP   +R+PGGSSSG+ VAV
Sbjct: 95  STASAVSALVESGATCIGTTVVDELAYGISGENKHFGTPTNPAVPNRVPGGSSSGAAVAV 154

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
           AA  VDFSLG DT G VRVPA +CGILGFRPSHG VS  G+IP++ S DTVGWFA+D  +
Sbjct: 155 AANFVDFSLGVDTSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDTVGWFAKDPDV 214

Query: 184 LKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQI 243
           L+RVG +LLQ P +    P ++ IA+DCF+ L N+  +R +Q  + + EKLFG  ++K I
Sbjct: 215 LRRVGHILLQAPFVMQRNPRQIIIADDCFQHL-NVPLDRTSQVVIKATEKLFGKQVLKHI 274

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           +   Y+  KV SLK   I++++G   S  SL  L    + LQRHEF   H EW+      
Sbjct: 275 NFEDYISSKVSSLKACSIQKSNGVLKS-SSLKLLANVMQSLQRHEFEHTHSEWMSIVKPD 334

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTD 363
           L P +S ++ E   EV++  I+ S+S++ ELR A+ +LL+D GVL IPTV  PPPKL   
Sbjct: 335 LHPAVSAQLHEKF-EVSELEIENSKSVRSELRVAVNSLLKDEGVLVIPTVADPPPKLGGK 394

Query: 364 ISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLY 423
               +D++++A SLL+IA++SG CQV+VPLG ++  PVS+SL+A+HG D FLL+ + ++Y
Sbjct: 395 EFLSHDYQSRALSLLSIASISGCCQVTVPLGFFDKNPVSVSLIARHGGDRFLLDTLKTMY 454

Query: 424 STLKEEVE 428
           + L+E+ +
Sbjct: 455 TVLQEQAD 459

BLAST of Csa1G570120 vs. Swiss-Prot
Match: OE64C_ARATH (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana GN=OEP64 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.2e-111
Identity = 205/425 (48.24%), Postives = 283/425 (66.59%), Query Frame = 1

Query: 4   DDYGAFIEKFLL----QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPAN 63
           +D+GAFI+K +L    Q + P    PL+GLTFAV D+FDI GYV GFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAV 123
            T+P V T++ GGATC+GKT++DE AFSI+GEN HY +P NP A  RIPGG+ SG+ VAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
           A   VDF+LG DT G VRVPA YCG+LGF+ S+G +S +G+IP++ S D+VGWFARD   
Sbjct: 152 ATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNT 211

Query: 184 LKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQI 243
           L+RVG +LLQ P      P ++ +A+DCF+LL  I  +R+TQ    S EKLFG  L+K  
Sbjct: 212 LRRVGHVLLQLPFATQRNPRQIILADDCFQLL-KIPVDRITQVVTKSAEKLFGRQLLKHQ 271

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           +L  Y E KVPSLK F   +       + +   L    + LQRHEF  NHG+W+ +    
Sbjct: 272 NLETYFETKVPSLKEFARTKAIA-NTKVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPA 331

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTD 363
           + P I  ++ E   E+T+E  +   +I+ E R A+ +LL+D G+L IPT+P  PPKL + 
Sbjct: 332 IDPVILSQVCE-NPELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSK 391

Query: 364 ISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLY 423
                D++ +A SLL+IA++SG CQV+VPLG +   P+S+S + +HG D FLL+ V ++Y
Sbjct: 392 EITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMY 451

Query: 424 STLKE 425
            +L+E
Sbjct: 452 PSLQE 453

BLAST of Csa1G570120 vs. Swiss-Prot
Match: OE64M_ARATH (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana GN=OM64 PE=1 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 6.0e-100
Identity = 190/429 (44.29%), Postives = 277/429 (64.57%), Query Frame = 1

Query: 4   DDYGAFIEKFLL----QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPAN 63
           +D+GAF+++F L        P+ K  LSGLTF++ D FD++ Y+ GFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAV 123
           +TA  V T+L+ GATC+GKTIMDE+ F I GEN HYGTP NP   D +PGG SSGS V+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
            A+LVDFSLG DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGWFA D ++
Sbjct: 165 GAELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSV 224

Query: 184 LKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQI 243
           L +VG  LL    + H +   +  A+D F+ LS+I  ++  Q    ++E L G    K +
Sbjct: 225 LCQVGHALLNLSAVTHRRQRSLIFADDLFE-LSDIPKQKSVQVVRKAIENLSGYKTPKHV 284

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           ++G+YV   VPSL  F  E++   ++S  +L AL      +QRHEF  NH EW ++  S 
Sbjct: 285 NVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSF 344

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTD 363
           LGP  S  ++  ++   +E+I     ++ E+R  + +LL++ G+L IPTV  PPP+LNT 
Sbjct: 345 LGPRFSNDVVTALKS-KNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPPRLNTK 404

Query: 364 ISEQY-DFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSL 423
            ++   +F  + ++L  IA++SG CQV++PLG +   P+S+SLL  +G D FLL+    +
Sbjct: 405 RNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDV 464

Query: 424 YSTLKEEVE 428
           Y++L+++ +
Sbjct: 465 YASLQDQAK 470

BLAST of Csa1G570120 vs. Swiss-Prot
Match: GATA_SULTO (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) GN=gatA PE=3 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 2.3e-35
Identity = 116/420 (27.62%), Postives = 210/420 (50.00%), Query Frame = 1

Query: 16  QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPAVLTILRGGATC 75
           ++ +P  KL  SG+  A+KD    +G      +       PP + T   +  + + GA  
Sbjct: 53  KLKNPKGKL--SGILIAIKDNISTKGIRTTCASRMLEDYVPPYDATV--IEKLKQEGAVI 112

Query: 76  IGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLVDFSLGTDTGGS 135
           +GKT MDE A     E  ++G  +NP   +R PGGSS GSG A+AA +VD +LG+DTGGS
Sbjct: 113 LGKTNMDEFAMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGIVDIALGSDTGGS 172

Query: 136 VRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVGWLLL------- 195
           +R PA+Y  + G +PS+G VS  G++    S + +G  A+++  L  +  ++        
Sbjct: 173 IRAPAAYNAVFGLKPSYGTVSRFGLVAYANSLEQIGPMAKNAEDLALLYSIISGDDPKDA 232

Query: 196 -------QEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFG-GHLIKQIS 255
                  Q PE    K  K+ + +D    +   + + +   F S+++KL   G +IK+++
Sbjct: 233 TTIHFEPQVPEKVELKDVKIAVLKD----IVEASDKPVVSIFNSTLDKLSSEGAIIKEVN 292

Query: 256 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 315
           LG Y E  +P+  +++I  ++    +  +LA     +    ++    + G W  ++  + 
Sbjct: 293 LG-YAEYALPA--YYIIAMSE----ASSNLARFDGVRYGYSKY----SEGNWRETFAKNR 352

Query: 316 GPG----ISERIL--EFIREV--TDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGP 375
           G G    +  RIL   FI      +E    +  I+  +++ +  +L++F ++A PT+P  
Sbjct: 353 GEGFGIEVKRRILLGSFILSAGYYEEFYIKALKIRRLIKDNVDNILKEFDLIASPTMPIL 412

Query: 376 PPKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLL 413
           PPK+   + +     A   + + IA ++GV  +S P G YN LP+ + L+ ++ +D +++
Sbjct: 413 PPKIGEVVEDPIKMYAMDLNTV-IANLAGVPALSQPAGFYNNLPIGLQLMGRYLSDYYIM 452

BLAST of Csa1G570120 vs. TrEMBL
Match: A0A0A0LZ24_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G570120 PE=4 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 3.3e-246
Identity = 430/430 (100.00%), Postives = 430/430 (100.00%), Query Frame = 1

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS
Sbjct: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420

Query: 421 TLKEEVEAIY 431
           TLKEEVEAIY
Sbjct: 421 TLKEEVEAIY 430

BLAST of Csa1G570120 vs. TrEMBL
Match: A0A0A0M1K1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G570110 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.3e-197
Identity = 345/430 (80.23%), Postives = 385/430 (89.53%), Query Frame = 1

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAV  YGAF+EKFLLQ SSPSD+LPL+GLTFAVKDIFD++G+V GFGNPEWLRTHPPAN 
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAP V TILRGGATCIG+TIMDEMA+SINGENFHYGTPQNP ASDR+PGGSSSGS VAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           A LVDFSLGTDTGGSVRVPASYCGILGFRPSHG VSTSGV+PM QS DTVGWFARD  +L
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTVGWFARDPVVL 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           K+VGWLLLQ+PE+EH KP +V IAEDCFK LS+I SERLTQAFV+SV+KLFGGH IK++S
Sbjct: 181 KKVGWLLLQQPEVEHQKPRQVLIAEDCFK-LSSIPSERLTQAFVNSVKKLFGGHPIKEVS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           LG YVE+KVPSLKHFMIE N G +HSIPSLAAL RS + LQR+EF INH EWVR+Y+ HL
Sbjct: 241 LGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISER+ E +R  TDENIDL  SI+IELR+ALAALLEDFGVLAIPTVPGPPPKLNTD+
Sbjct: 301 GPGISERVSEAMR-ATDENIDLCHSIRIELRKALAALLEDFGVLAIPTVPGPPPKLNTDV 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SE  DFRAKAFSLL+IA VSG CQVS+PLGLYNGLPVSISL+A HG+DGFLLN+V SLY+
Sbjct: 361 SELQDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYN 420

Query: 421 TLKEEVEAIY 431
           TL+EEV+A +
Sbjct: 421 TLEEEVKASF 428

BLAST of Csa1G570120 vs. TrEMBL
Match: B9RF45_RICCO (Amidase, putative OS=Ricinus communis GN=RCOM_1431340 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 7.7e-163
Identity = 288/422 (68.25%), Postives = 344/422 (81.52%), Query Frame = 1

Query: 5   DYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPA 64
           DYGAF+EKF+L+ SS SD+LPL  LTFAVKDIFD++GYV GFGNP+W RTH  A  TAPA
Sbjct: 78  DYGAFMEKFVLKPSSSSDQLPLHSLTFAVKDIFDVDGYVTGFGNPDWARTHSAATATAPA 137

Query: 65  VLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLV 124
           VL +LRGGATC+GK IMDEMA+SINGEN HYGTP NP A DR+PGGSSSGS VAV AKLV
Sbjct: 138 VLAVLRGGATCVGKVIMDEMAYSINGENAHYGTPTNPSAPDRVPGGSSSGSAVAVGAKLV 197

Query: 125 DFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVG 184
           DFSLG+DTGGSVRVPASYCGILGFRPSHGVVS SGVIPM QSFDTVGWF+R+  ILK VG
Sbjct: 198 DFSLGSDTGGSVRVPASYCGILGFRPSHGVVSVSGVIPMAQSFDTVGWFSRNPVILKHVG 257

Query: 185 WLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQISLGKY 244
            LLLQ P+++  KP ++FIAEDCF+ LS+I  +R++Q  V SVEKLFGG ++K ++LG Y
Sbjct: 258 RLLLQLPDVDRTKPNQIFIAEDCFQ-LSSIPKKRVSQVLVKSVEKLFGGDIVKHVNLGDY 317

Query: 245 VEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHLGPGI 304
           VEDKVPSL+HFM +E    +++I  LAAL  + R LQR+EF  NHGEWV +     GPG+
Sbjct: 318 VEDKVPSLRHFMSKEIKEQEYNIAPLAALSSAMRMLQRYEFKTNHGEWVTTVKPDFGPGL 377

Query: 305 SERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDISEQY 364
            ERI E +R  T+ENID  +S++ ELR AL  LLE+FG++AIPTVPG PPKLNTD +   
Sbjct: 378 KERIWEAVRS-TEENIDAFQSVRTELRAALTNLLEEFGIIAIPTVPGVPPKLNTDPTTLE 437

Query: 365 DFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYSTLKE 424
            FRAKAFSLL+I  VSG CQVS+PLG+++GLPVSISLLAKHG+D FLLN+V++LY TL+E
Sbjct: 438 IFRAKAFSLLSITGVSGFCQVSIPLGMHDGLPVSISLLAKHGSDVFLLNVVETLYDTLQE 497

Query: 425 EV 427
           EV
Sbjct: 498 EV 497

BLAST of Csa1G570120 vs. TrEMBL
Match: A5BL97_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015334 PE=4 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 7.2e-161
Identity = 284/423 (67.14%), Postives = 339/423 (80.14%), Query Frame = 1

Query: 5   DYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPA 64
           DYGAF+E+F+LQ SS S +LPL+GLTFAVKDIFD++GYV GFGNP+W RTH  A  TAP+
Sbjct: 6   DYGAFMERFILQPSSSSHELPLNGLTFAVKDIFDVDGYVTGFGNPDWARTHQAAMLTAPS 65

Query: 65  VLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLV 124
           VL +L+GGATC+GKT+MDEMA+SINGEN HYGTP NP A DR+PGGSSSGS VAV A LV
Sbjct: 66  VLAVLKGGATCVGKTVMDEMAYSINGENKHYGTPTNPQAPDRVPGGSSSGSAVAVGAMLV 125

Query: 125 DFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVG 184
           DFSLGTDTGGSVRVPASYCGI G RPSHGVVST+GVIPM QSFDTVGWFARD  IL RVG
Sbjct: 126 DFSLGTDTGGSVRVPASYCGIFGIRPSHGVVSTTGVIPMAQSFDTVGWFARDPEILNRVG 185

Query: 185 WLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQISLGKY 244
            +LL  P++   KP+++ I EDCF+LLS I  +R+TQ  + SVEKLFG  ++K +SLG Y
Sbjct: 186 HVLLPFPDVNPVKPSQIIIPEDCFRLLS-IPIDRVTQVLIKSVEKLFGSDIVKHVSLGDY 245

Query: 245 VEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHLGPGI 304
           VEDKV SLK FM +EN   +++IPSLAAL  + R LQR+EF  NHGEWVR+    LGPGI
Sbjct: 246 VEDKVTSLKPFMSKENQTQEYNIPSLAALSTAMRLLQRYEFKNNHGEWVRAVQPDLGPGI 305

Query: 305 SERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDISEQY 364
            ER+ E +   TDE ID+ +S++ E R AL  LL D GVL +PTVPGPPPKL TD +   
Sbjct: 306 RERVWEAL-GTTDEKIDVCQSLKTEFRSALTDLLGDSGVLVLPTVPGPPPKLQTDPTTLE 365

Query: 365 DFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYSTLKE 424
            FRA+AFSLL+IA VSG CQVS+PLGLY+ LPV++SLLAKHG+DGFLLNLV +LY ++KE
Sbjct: 366 SFRARAFSLLSIAGVSGFCQVSIPLGLYDNLPVAVSLLAKHGSDGFLLNLVQTLYGSIKE 425

Query: 425 EVE 428
           +VE
Sbjct: 426 QVE 426

BLAST of Csa1G570120 vs. TrEMBL
Match: D7U1Z1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0128g00320 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 3.6e-160
Identity = 283/423 (66.90%), Postives = 338/423 (79.91%), Query Frame = 1

Query: 5   DYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPA 64
           +YGAF+E+F+LQ SS S +LPL+GLTFAVKDIFD++GYV GFGNP+W RTH  A  TAP+
Sbjct: 6   NYGAFMERFILQPSSSSHELPLNGLTFAVKDIFDVDGYVTGFGNPDWARTHQAAMLTAPS 65

Query: 65  VLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLV 124
           VL +L+GGATC+GKT+MDEMA+SINGEN HYGTP NP A DR+PGGSSSGS VAV A LV
Sbjct: 66  VLAVLKGGATCVGKTVMDEMAYSINGENKHYGTPTNPQAPDRVPGGSSSGSAVAVGAMLV 125

Query: 125 DFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVG 184
           DFSLGTDTGGSVRVPASYCGI G RPSHGVVST+GVIPM QSFDTVGWFARD  IL RVG
Sbjct: 126 DFSLGTDTGGSVRVPASYCGIFGIRPSHGVVSTTGVIPMAQSFDTVGWFARDPEILNRVG 185

Query: 185 WLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQISLGKY 244
            +LL  P++   KP+++ I EDCF+LLS I  +R+TQ  + SVEKLFG  ++K +SLG Y
Sbjct: 186 HVLLPFPDVNPVKPSQIIIPEDCFRLLS-IPIDRVTQVLIKSVEKLFGSDIVKHVSLGDY 245

Query: 245 VEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHLGPGI 304
           VEDKV SLK FM +EN   +++IPSLAAL  + R LQR+EF  NHGEWVR     LGPGI
Sbjct: 246 VEDKVTSLKPFMSKENQTQEYNIPSLAALSTAMRLLQRYEFKNNHGEWVREVQPDLGPGI 305

Query: 305 SERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDISEQY 364
            ER+ E +   TDE ID+ +S++ E R AL  LL D GVL +PTVPGPPPKL TD +   
Sbjct: 306 RERVWEAL-GTTDEKIDVCQSLKTEFRSALTDLLGDSGVLVLPTVPGPPPKLQTDPTTLE 365

Query: 365 DFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYSTLKE 424
            FRA+AFSLL+IA VSG CQVS+PLGLY+ LPV++SLLAKHG+DGFLLNLV +LY ++KE
Sbjct: 366 SFRARAFSLLSIAGVSGFCQVSIPLGLYDNLPVAVSLLAKHGSDGFLLNLVQTLYGSIKE 425

Query: 425 EVE 428
           +VE
Sbjct: 426 QVE 426

BLAST of Csa1G570120 vs. TAIR10
Match: AT1G08980.1 (AT1G08980.1 amidase 1)

HSP 1 Score: 509.6 bits (1311), Expect = 1.9e-144
Identity = 262/419 (62.53%), Postives = 317/419 (75.66%), Query Frame = 1

Query: 4   DDYGAFIEKFLLQMSSPSDKLP-LSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTA 63
           +D+GAFIEK  +  +S S   P L GLTFA+KDIFD+EG V GFGNP+WLRTH  A  TA
Sbjct: 5   NDFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTA 64

Query: 64  PAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAK 123
           P V ++L  GAT +G TIMDEMA+SINGEN HYGTP+NP A DR+PGGSSSGS VAVAA+
Sbjct: 65  PVVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAAR 124

Query: 124 LVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKR 183
           LVDFS+GTDTGGSVRVPASYCGI GFRPSHG VST G+ PM QSFDTVGWFARD+A LKR
Sbjct: 125 LVDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKR 184

Query: 184 VGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGH-LIKQISL 243
           VG +LLQ+  +   +P+++ IA+DCFKL S +  + L Q  V SVEK FGG+ ++K+++L
Sbjct: 185 VGCVLLQQHHLNPIEPSQLIIADDCFKLCS-VPHDLLVQPLVGSVEKSFGGNTVVKKVNL 244

Query: 244 GKYVEDKVPSLKHFMIEEN--DGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           G+Y+   VPSLKHFM  ++     +  IPSL AL  S R LQRHEF INHG W+ S    
Sbjct: 245 GEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPE 304

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTD 363
            GPGISERI E IR  +DE ID  RS++ EL  AL+ LL + GVL IPTVPGPPP L  +
Sbjct: 305 FGPGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQAN 364

Query: 364 ISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSL 419
           ++    FR++AFSLL+IA VSG CQVS+PLGL+  LPVS+SL+AK+G+DGFLL+LVDSL
Sbjct: 365 VAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of Csa1G570120 vs. TAIR10
Match: AT3G17970.1 (AT3G17970.1 translocon at the outer membrane of chloroplasts 64-III)

HSP 1 Score: 404.8 bits (1039), Expect = 6.5e-113
Identity = 205/425 (48.24%), Postives = 283/425 (66.59%), Query Frame = 1

Query: 4   DDYGAFIEKFLL----QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPAN 63
           +D+GAFI+K +L    Q + P    PL+GLTFAV D+FDI GYV GFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAV 123
            T+P V T++ GGATC+GKT++DE AFSI+GEN HY +P NP A  RIPGG+ SG+ VAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
           A   VDF+LG DT G VRVPA YCG+LGF+ S+G +S +G+IP++ S D+VGWFARD   
Sbjct: 152 ATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNT 211

Query: 184 LKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQI 243
           L+RVG +LLQ P      P ++ +A+DCF+LL  I  +R+TQ    S EKLFG  L+K  
Sbjct: 212 LRRVGHVLLQLPFATQRNPRQIILADDCFQLL-KIPVDRITQVVTKSAEKLFGRQLLKHQ 271

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           +L  Y E KVPSLK F   +       + +   L    + LQRHEF  NHG+W+ +    
Sbjct: 272 NLETYFETKVPSLKEFARTKAIA-NTKVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPA 331

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTD 363
           + P I  ++ E   E+T+E  +   +I+ E R A+ +LL+D G+L IPT+P  PPKL + 
Sbjct: 332 IDPVILSQVCE-NPELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSK 391

Query: 364 ISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLY 423
                D++ +A SLL+IA++SG CQV+VPLG +   P+S+S + +HG D FLL+ V ++Y
Sbjct: 392 EITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMY 451

Query: 424 STLKE 425
            +L+E
Sbjct: 452 PSLQE 453

BLAST of Csa1G570120 vs. TAIR10
Match: AT5G09420.1 (AT5G09420.1 translocon at the outer membrane of chloroplasts 64-V)

HSP 1 Score: 365.9 bits (938), Expect = 3.4e-101
Identity = 190/429 (44.29%), Postives = 277/429 (64.57%), Query Frame = 1

Query: 4   DDYGAFIEKFLL----QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPAN 63
           +D+GAF+++F L        P+ K  LSGLTF++ D FD++ Y+ GFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAV 123
           +TA  V T+L+ GATC+GKTIMDE+ F I GEN HYGTP NP   D +PGG SSGS V+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
            A+LVDFSLG DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGWFA D ++
Sbjct: 165 GAELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSV 224

Query: 184 LKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQI 243
           L +VG  LL    + H +   +  A+D F+ LS+I  ++  Q    ++E L G    K +
Sbjct: 225 LCQVGHALLNLSAVTHRRQRSLIFADDLFE-LSDIPKQKSVQVVRKAIENLSGYKTPKHV 284

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           ++G+YV   VPSL  F  E++   ++S  +L AL      +QRHEF  NH EW ++  S 
Sbjct: 285 NVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSF 344

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTD 363
           LGP  S  ++  ++   +E+I     ++ E+R  + +LL++ G+L IPTV  PPP+LNT 
Sbjct: 345 LGPRFSNDVVTALKS-KNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPPRLNTK 404

Query: 364 ISEQY-DFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSL 423
            ++   +F  + ++L  IA++SG CQV++PLG +   P+S+SLL  +G D FLL+    +
Sbjct: 405 RNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDV 464

Query: 424 YSTLKEEVE 428
           Y++L+++ +
Sbjct: 465 YASLQDQAK 470

BLAST of Csa1G570120 vs. TAIR10
Match: AT3G25660.1 (AT3G25660.1 Amidase family protein)

HSP 1 Score: 104.0 bits (258), Expect = 2.4e-22
Identity = 60/149 (40.27%), Postives = 78/149 (52.35%), Query Frame = 1

Query: 25  PLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGKTIMDEM 84
           PL+G+   VKD    +G  +   +       PP + TA  V  I   G   +GKT MDE 
Sbjct: 107 PLAGVLIGVKDNICTQGMPSTAASRILEHYRPPFDATA--VKKIKELGGIVVGKTNMDEF 166

Query: 85  AFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLVDFSLGTDTGGSVRVPASYCG 144
                 E   +    NP    R+PGGSS GS  AVAA+    SLG+DTGGSVR PAS+CG
Sbjct: 167 GMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCG 226

Query: 145 ILGFRPSHGVVSTSGVIPMTQSFDTVGWF 174
           ++G +P++G VS  G++    S D +G F
Sbjct: 227 VVGLKPTYGRVSRFGLMAYASSLDVIGCF 253

BLAST of Csa1G570120 vs. TAIR10
Match: AT5G64440.1 (AT5G64440.1 fatty acid amide hydrolase)

HSP 1 Score: 99.4 bits (246), Expect = 5.9e-21
Identity = 102/407 (25.06%), Postives = 161/407 (39.56%), Query Frame = 1

Query: 26  LSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGKTIMDEMA 85
           L G+   +KD  D   +    G   WL       + +  V  +   GA  +GK  M E+ 
Sbjct: 197 LDGIFVTIKDDIDCLPHPTN-GGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 256

Query: 86  FSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLVDFSLGTDTGGSVRVPASYCGI 145
               G N +YGT +NPH   R  GGSSSGS   VAA L   +LGTD GGSVR+P++ CGI
Sbjct: 257 MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSALCGI 316

Query: 146 LGFRPSHGVVSTSGVIPMTQSFDTVGWFAR--DSAILKRVGWLLLQEPEIEHYKPTKVFI 205
            G + ++G    +G +    + + +G  A   + A L     L     +  + KP     
Sbjct: 317 TGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSADRYNLKP----- 376

Query: 206 AEDCF-KLLSNITSERLTQAFVSSVEKLFGGHLIKQISLGKYVEDKVPSLKHFMIEENDG 265
           +  CF KLLS+  S  +    +    K F       IS      DK   +   ++  N G
Sbjct: 377 SPPCFPKLLSHNGSNAIGSLRLGKYTKWFNDVSSSDIS------DKCEDILK-LLSNNHG 436

Query: 266 YK---HSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHLGPGISERI-------LEFI 325
            K     +P L      +     H  +I     + S   +   G + ++           
Sbjct: 437 CKVVEIVVPEL------EEMRAAHVISIG-SPTLSSLTPYCEAGKNSKLSYDTRTSFAIF 496

Query: 326 REVTDENIDLSRSIQIELREALAALLEDFGVLAIP----TVPGPPPKLNTDISEQYDFRA 385
           R  +  +   ++ ++  L E    + +D  V+  P    T P  PP    +         
Sbjct: 497 RSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDALKNGETNIQVTT 556

Query: 386 KAFSLLTIAAVSGVCQVSVPLGL-YNGLPVSISLLAKHGADGFLLNL 415
                +  A + G   +SVP+G    GLP+ + ++ +  A+  +L L
Sbjct: 557 DLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGL 583

BLAST of Csa1G570120 vs. NCBI nr
Match: gi|449435685|ref|XP_004135625.1| (PREDICTED: amidase 1-like [Cucumis sativus])

HSP 1 Score: 858.6 bits (2217), Expect = 4.7e-246
Identity = 430/430 (100.00%), Postives = 430/430 (100.00%), Query Frame = 1

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS
Sbjct: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420

Query: 421 TLKEEVEAIY 431
           TLKEEVEAIY
Sbjct: 421 TLKEEVEAIY 430

BLAST of Csa1G570120 vs. NCBI nr
Match: gi|659099553|ref|XP_008450659.1| (PREDICTED: amidase 1-like [Cucumis melo])

HSP 1 Score: 820.5 bits (2118), Expect = 1.4e-234
Identity = 410/430 (95.35%), Postives = 420/430 (97.67%), Query Frame = 1

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDD GAFIEKFLLQMSSPSD+LPLSGLTFAVKDIFDIEG+VAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKT+MDEMAFSINGENFHYGTPQNP AS RIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCG+LGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVGWLLLQEPE+EHYKPTKVFIAEDCFKLLS+ITSERLTQAFVSSVEKLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKR LQRHEF INHGEWV SY SHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISE+ILEFIRE TDENIDLSRSIQIELRE LAALLEDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SEQ+DFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLNLVD LYS
Sbjct: 361 SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS 420

Query: 421 TLKEEVEAIY 431
           TLKEEVEAIY
Sbjct: 421 TLKEEVEAIY 430

BLAST of Csa1G570120 vs. NCBI nr
Match: gi|449435683|ref|XP_004135624.1| (PREDICTED: amidase 1 [Cucumis sativus])

HSP 1 Score: 697.2 bits (1798), Expect = 1.8e-197
Identity = 345/430 (80.23%), Postives = 385/430 (89.53%), Query Frame = 1

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAV  YGAF+EKFLLQ SSPSD+LPL+GLTFAVKDIFD++G+V GFGNPEWLRTHPPAN 
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAP V TILRGGATCIG+TIMDEMA+SINGENFHYGTPQNP ASDR+PGGSSSGS VAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           A LVDFSLGTDTGGSVRVPASYCGILGFRPSHG VSTSGV+PM QS DTVGWFARD  +L
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTVGWFARDPVVL 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           K+VGWLLLQ+PE+EH KP +V IAEDCFK LS+I SERLTQAFV+SV+KLFGGH IK++S
Sbjct: 181 KKVGWLLLQQPEVEHQKPRQVLIAEDCFK-LSSIPSERLTQAFVNSVKKLFGGHPIKEVS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           LG YVE+KVPSLKHFMIE N G +HSIPSLAAL RS + LQR+EF INH EWVR+Y+ HL
Sbjct: 241 LGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISER+ E +R  TDENIDL  SI+IELR+ALAALLEDFGVLAIPTVPGPPPKLNTD+
Sbjct: 301 GPGISERVSEAMR-ATDENIDLCHSIRIELRKALAALLEDFGVLAIPTVPGPPPKLNTDV 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SE  DFRAKAFSLL+IA VSG CQVS+PLGLYNGLPVSISL+A HG+DGFLLN+V SLY+
Sbjct: 361 SELQDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYN 420

Query: 421 TLKEEVEAIY 431
           TL+EEV+A +
Sbjct: 421 TLEEEVKASF 428

BLAST of Csa1G570120 vs. NCBI nr
Match: gi|659099551|ref|XP_008450658.1| (PREDICTED: amidase 1-like [Cucumis melo])

HSP 1 Score: 692.2 bits (1785), Expect = 5.8e-196
Identity = 345/430 (80.23%), Postives = 385/430 (89.53%), Query Frame = 1

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAV  YGAF+EKFLLQ SSPSD+LPL+GLTFAVKDIFD++G+V GFGNPEWLRTHPPAN 
Sbjct: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAP V TILRGGATCIG+TIMDEMA+SINGENFHYGTPQNP ASDR+PGGSSSGS VAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           A LVDFSLGTDTGGSVRVPASYCGILGFRPSHG VSTSGV+PM QSFDTVGWFARD  + 
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVF 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           K+VG LLLQ+PE+EH KPT+V IAEDCFK LS+I SERLTQAFV+SV+KLFGGHLIKQ++
Sbjct: 181 KKVGLLLLQQPEVEHQKPTQVLIAEDCFK-LSSIPSERLTQAFVNSVKKLFGGHLIKQVN 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           LG YVE+KVPSLK FMIE N G +HSIPSLAAL RS + LQR+EF INH EWVR+Y+ HL
Sbjct: 241 LGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISER+ E +R  TDENIDL  SI+IELR ALAALLEDFGVLAIPTVPGPPPKLNTD+
Sbjct: 301 GPGISERVSEAMR-ATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNTDV 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SE +DFRAKAFSLL+IA VSG CQVS+PLGLYNGLPVSISL+A HG+DGFLLN+V SLY+
Sbjct: 361 SELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYN 420

Query: 421 TLKEEVEAIY 431
           TL+EEV+A +
Sbjct: 421 TLEEEVKASF 427

BLAST of Csa1G570120 vs. NCBI nr
Match: gi|223548325|gb|EEF49816.1| (amidase, putative [Ricinus communis])

HSP 1 Score: 581.6 bits (1498), Expect = 1.1e-162
Identity = 288/422 (68.25%), Postives = 344/422 (81.52%), Query Frame = 1

Query: 5   DYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPA 64
           DYGAF+EKF+L+ SS SD+LPL  LTFAVKDIFD++GYV GFGNP+W RTH  A  TAPA
Sbjct: 78  DYGAFMEKFVLKPSSSSDQLPLHSLTFAVKDIFDVDGYVTGFGNPDWARTHSAATATAPA 137

Query: 65  VLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLV 124
           VL +LRGGATC+GK IMDEMA+SINGEN HYGTP NP A DR+PGGSSSGS VAV AKLV
Sbjct: 138 VLAVLRGGATCVGKVIMDEMAYSINGENAHYGTPTNPSAPDRVPGGSSSGSAVAVGAKLV 197

Query: 125 DFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVG 184
           DFSLG+DTGGSVRVPASYCGILGFRPSHGVVS SGVIPM QSFDTVGWF+R+  ILK VG
Sbjct: 198 DFSLGSDTGGSVRVPASYCGILGFRPSHGVVSVSGVIPMAQSFDTVGWFSRNPVILKHVG 257

Query: 185 WLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQISLGKY 244
            LLLQ P+++  KP ++FIAEDCF+ LS+I  +R++Q  V SVEKLFGG ++K ++LG Y
Sbjct: 258 RLLLQLPDVDRTKPNQIFIAEDCFQ-LSSIPKKRVSQVLVKSVEKLFGGDIVKHVNLGDY 317

Query: 245 VEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHLGPGI 304
           VEDKVPSL+HFM +E    +++I  LAAL  + R LQR+EF  NHGEWV +     GPG+
Sbjct: 318 VEDKVPSLRHFMSKEIKEQEYNIAPLAALSSAMRMLQRYEFKTNHGEWVTTVKPDFGPGL 377

Query: 305 SERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDISEQY 364
            ERI E +R  T+ENID  +S++ ELR AL  LLE+FG++AIPTVPG PPKLNTD +   
Sbjct: 378 KERIWEAVRS-TEENIDAFQSVRTELRAALTNLLEEFGIIAIPTVPGVPPKLNTDPTTLE 437

Query: 365 DFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYSTLKE 424
            FRAKAFSLL+I  VSG CQVS+PLG+++GLPVSISLLAKHG+D FLLN+V++LY TL+E
Sbjct: 438 IFRAKAFSLLSITGVSGFCQVSIPLGMHDGLPVSISLLAKHGSDVFLLNVVETLYDTLQE 497

Query: 425 EV 427
           EV
Sbjct: 498 EV 497

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AMI1_ARATH3.3e-14362.53Amidase 1 OS=Arabidopsis thaliana GN=AMI1 PE=1 SV=1[more]
TOC64_PEA2.9e-11549.53Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum GN=TOC64 PE... [more]
OE64C_ARATH1.2e-11148.24Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana GN=OEP64 PE=1 S... [more]
OE64M_ARATH6.0e-10044.29Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana GN=OM64 PE=1 SV... [more]
GATA_SULTO2.3e-3527.62Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Sulfolobus tokodaii (strain DSM... [more]
Match NameE-valueIdentityDescription
A0A0A0LZ24_CUCSA3.3e-246100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G570120 PE=4 SV=1[more]
A0A0A0M1K1_CUCSA1.3e-19780.23Uncharacterized protein OS=Cucumis sativus GN=Csa_1G570110 PE=4 SV=1[more]
B9RF45_RICCO7.7e-16368.25Amidase, putative OS=Ricinus communis GN=RCOM_1431340 PE=4 SV=1[more]
A5BL97_VITVI7.2e-16167.14Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015334 PE=4 SV=1[more]
D7U1Z1_VITVI3.6e-16066.90Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0128g00320 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT1G08980.11.9e-14462.53 amidase 1[more]
AT3G17970.16.5e-11348.24 translocon at the outer membrane of chloroplasts 64-III[more]
AT5G09420.13.4e-10144.29 translocon at the outer membrane of chloroplasts 64-V[more]
AT3G25660.12.4e-2240.27 Amidase family protein[more]
AT5G64440.15.9e-2125.06 fatty acid amide hydrolase[more]
Match NameE-valueIdentityDescription
gi|449435685|ref|XP_004135625.1|4.7e-246100.00PREDICTED: amidase 1-like [Cucumis sativus][more]
gi|659099553|ref|XP_008450659.1|1.4e-23495.35PREDICTED: amidase 1-like [Cucumis melo][more]
gi|449435683|ref|XP_004135624.1|1.8e-19780.23PREDICTED: amidase 1 [Cucumis sativus][more]
gi|659099551|ref|XP_008450658.1|5.8e-19680.23PREDICTED: amidase 1-like [Cucumis melo][more]
gi|223548325|gb|EEF49816.1|1.1e-16268.25amidase, putative [Ricinus communis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000120Amidase
IPR020556Amidase_CS
IPR023631Amidase_dom
Vocabulary: Molecular Function
TermDefinition
GO:0016884carbon-nitrogen ligase activity, with glutamine as amido-N-donor
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006525 arginine metabolic process
biological_process GO:0018874 benzoate metabolic process
biological_process GO:0030154 cell differentiation
biological_process GO:0019761 glucosinolate biosynthetic process
biological_process GO:0009684 indoleacetic acid biosynthetic process
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0006558 L-phenylalanine metabolic process
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0006560 proline metabolic process
biological_process GO:0042207 styrene catabolic process
biological_process GO:0006568 tryptophan metabolic process
cellular_component GO:0005829 cytosol
molecular_function GO:0004040 amidase activity
molecular_function GO:0016884 carbon-nitrogen ligase activity, with glutamine as amido-N-donor
molecular_function GO:0043864 indoleacetamide hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa1G570120.1Csa1G570120.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000120AmidasePANTHERPTHR11895AMIDASEcoord: 4..429
score: 3.6E
IPR020556Amidase, conserved sitePROSITEPS00571AMIDASEScoord: 109..140
scor
IPR023631Amidase signature domainGENE3DG3DSA:3.90.1300.10coord: 21..418
score: 5.0
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 20..176
score: 1.8E-51coord: 316..412
score: 1.
IPR023631Amidase signature domainunknownSSF75304Amidase signature (AS) enzymescoord: 12..414
score: 9.16
NoneNo IPR availablePANTHERPTHR11895:SF39OUTER ENVELOPE PROTEIN 64, CHLOROPLASTICcoord: 4..429
score: 3.6E

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None