Csa1G542410 (gene) Cucumber (Chinese Long) v2

NameCsa1G542410
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionDNA primase/helicase; contains IPR006171 (Toprim domain)
LocationChr1 : 20050526 .. 20058498 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAGACCTCCAGGTGCTATTATTGGAACTTTTTATGGTTGTTTAATTGTAGTTGGATTTATGATTATTTCTGCTTAATACTCATGTTGTGGGGTAACTTGATGTTTCTTGGCTGGGTGGTTACTCTTTTTTTTTCCCCTCTTTTTGGATTGGGATGAAATAGGATTTTTGAAGGCAGAAAAGTAGTTTGCAGGCAATGTATTTTGAGGATGGGACTTGGGAGAGGACTTGACGTTTATTTAGTTTTAATGTTATTCGCTTTCTTCTTTGTGCAGCCCTTTTGGGGAATCCACCAGACAAGGCTTCAATCTCCACTCGATTGAATATTCTAAGAAAAAAATTGCAGGATCTTGACATTGATATCGAAGCTTGTGTTCCTGGGCAGTTTTCCAGTTTGCTTTGTCCAATGGTATTTTGCTTCTAATATCAGTTTCTTTATGCACACTATGTTCTGTTTTGAGATATTCATCCAATTTGTAGAAGTGAAATGAAAATAGCTGCCAACTTCTATATCTTATTTTTTGTACAACGTAAACATTTAGAGGCCAATTTTAGAATTACTGTATGAACTTATAACTTCCAAGTGCAAAGAGGTTGGTATTATCATTATTATATTTATTTTAAAATTTTAATTTTTTTAACTTTACTCGATTAATTATGTATCAAATTGGTGCCAGTGTAAAGGTGGTGATACAAAGGAACGATCCTTCTCCCTAAATATCTCAGAAGATGGGTAAAGATTTTATAGCTTCTCTCTCGTCGAGTTTTTCTTAGAATCTTTTTATTTGATGTACTCATAGGCTTTGTGTGTTTGTGTTTTTCTTATATGGTTTGTTTTAAATGTTTTTGTGGAAGTTATTTAGGGTGACTTGTAATTATTAAGCTTCTTCCTCAGTTGAGTTTAAGTCATTTTACTTTTTATACTATTAGTATTTGTTATTTTTTTTGAAGAGGAAAGAAGTCTCAATATTATTAATGAATTAAATAGAGACAAATGCTCATAGTACTAGAGGGTATGGGCAAAGAAAGAGAGAGAGGCAGATCAACAAACGCACCCGGACATCTCATTTAGGTTGACACTCCTTAGCGCCCTCATCATGTCCATAGTGTGGAAAGAAATAAAAATGATCTTTTCTCTAGGTTGATAGTAGTCAATACAAAGCCAAAATACAAGCCCAAAATAAAGTAGTCAGTCCATACAGAACATTAGAAAAAAAGCATCAACGATATATTAGAAAAACATTCCTCGTCTCTGCATAGATCCATCTCCTGCCAGATAAGTGAGAATTTGTTAAAAAAGGATGTAACATCCATGGTCCCTTGCCTGCATTCATGAACTAGTTTCTGTACAGTATATTGATGAGACATTTTGATGTTTGGAATACAGTTTTTGGGCTGTGTCCTAAGTATGTTTAGGGTAAAGGTTTGCCAATCTGTGGCTCCATGCTATTAATTAATGTGGATCAAAGAAGAGAATCTTCATGCCCTTCTCCTTTACCTTCCTTGGCTTTTATCTCTTTCTTTGAACTAACTTTGGCCCACCGTGGTCCCCTATCTCCTTCCTCACTGTCTAAGTTTCCCTTCACACGTGCTTCCTCTTTCTCTTACGCATTCTACTGTATTGTAGAATAATAGGATGTCTACCATATTGTATCATAAAACAACATCAAAAAAGGAGAAGAAAAACAAAACAAAAAGACAAACTATACCATATAATATACAATCAATATCAAATCAAGTAATAATTTTAAGTCATTTAAAATTGGCAACTCAACAACCTCACGCAACTTTTCTTTTAGTCAAATAATGGTCAATTTAAAATAGGGGTCATTTATGACTATTTTCCAAAACTCAAGGACTAAGTGTTTACTTGGGAGTTAAATAGACATCAACACTAATCCTCAAGGACCAAGTATATGTTTCCCTACGTTTTGTAACAATCTTTTTGGTTATCAATTTAATTTTATTATAAATATGATTATTTTCAAATTCTTTATTTATTTTTTCTTGATATACATATAAGGCTGTGACAAGTTGATGGACTTAAACTCCATAGTTCGTTCACTACTAACCTTAGTTTGATTCTTAGCTTTTTTTTTTTTTTTTGTTCAATGAAATTAATACTTTTTTTCTAATGAAAAGTGGTTGGATTTCCTTCCCATCCACTGGACTTTTCAATTTTTCAATTGCATTGACCTGAATGTATTCTACGGCCTTTTTCTTTTTGAGTATCACATGTTTCTAATTTCCATTTCAAATGACCAGGGAGGCTGCTGTTTGGAATTGTTTTCGTGGAAAATGTGGTTGGAAAGGCCATACTCTGGTATTTATAATAATTCAATTAGTTATGCTTTCACACAAAATGTGGTTTGGGCCCTTTGACGTAGGGTTGATTTGAATAGTTCTTCAGTTATGTTTTGCTTTGTTTTTTTCTTTCTTTTTTATATATATATATTTTTATGAGATAATTTGGTTTTTGTTTCAATTTCTTGTATTTTGTTCAAATATCATAGAATGTTCCATAAAGTTTAATTGAGTGGTATCCTTACTACTTGTGGGAAGGGGTTGACGATGAAGGGTCTCATTTGGTTGATTGGAAATTAGTGGTTAAGCTAGTGGAACTAGTAGGATTAAGGATTCTTCATGGATTGCTTTGTGGTATAAAGTTATTGTGAGCAGATATGCCTCACACCCATTGATTTTTAAAGCTCAAGGCACAACAGTCTTCTGGGCACACGCACAAGTCGCAAAAGAAAAAGCTTAAGCTTTTTTGTTTAGGTGTACAGTACTACATTAAGAAGAGAGCCTAGACAAAACCCTTAGTTATTACTAGTTTTTTTTTTAAAAAAATAATGAAGACCCTCCAAACGCAGGGTTTTGAGCCTTTGGGCATCACAAAAGACGCATGAATGAGACGCTTCTTGTTTTGTGAGTCTCGCCTTTTCCAGCAAGGCACCAAACTTGGGCTTTGAGCCTAGGTGCGCACCCGAGTGGGCTTTTCAAAAACCGTCCAGAAAGCAGTTGTTCTGTCTAAATACATAAGAAAAATGAAAAATCTATTGGAAGCTATCTGATAGATACCTACATTTAGATAGGGGTAAGGGTAGTTAGTTATATAAGGCAGTTAGATGGTTATAAATAGGGAGGTGGGGAGGGAAGAAGGGCATGAAGAATTTGGTGAAGAATTAGGGCTGCAACATTCCTTGAAAGAGAGGAAGGGCAGAGGGTTAGGGTTCTTTTACTTTTCTTTTGCTGCTTTTATATTGTAATTTTCTGTTTGAGATATCAATAATTTTTTTTTTGAAATGGAGACGAGCTTCTTTATTAATAAAGGAAAATGAGACTAAAGCTCAAAGTACAAGAGAATTATACAAAGAGCAAAAAGTAAAGACAAACAACCAAACAAACTGCCCAAAGACAACCTCAGCGAATACAAGGAAAAAACGACTAAAACAAAACAAATGCAAAGAACAAGCTAAAAAGGCAAAAACCAACAGAAAAAAATGAAGATAAAACAGAACCAAAACGAAGAGAAATAAATACAAAGCATAACAAAATAACTTTCTCAAAAGGAAACCATTTGGATCTAGAGACTAGCAAAAAGAAGCAACCGGTGAACTCAAAGCTAACTTGCCAAAGGAGACCAAAACCAGAAGAGGAACAAAAGAATCTTTCATAATATCAGCCTCAGCAAAAGGTTGTACATCCTATACAAAGCTTTAACACACCATGTTGAATCTTCAAATTGATGTTCCAATTGAAAACTCATCCCCCATGCATGAAGAAGTACTAAAGATATATACCAAAATATTTCTCCCACAAGCCACTTTAATAGATTCCAGCACCTTTTTCAACCATAACTTGATTCATGACATATCGATAAAATAGAAACACTATATCGGTGTTCTATCACTATCACATATGGATTCCCTTTATTCTCTCAATTTCTCAAATGGTTTGTTGGGCAAGGCTTGAAAAGTTTATTTTGGGAGATCGTTGGGCAGGTGGTTATAAAACTTATATTATTAAAATAATAGAAAACCCGTTGGCGTGTCTATTATTTTATTTATACTTTTTTACTTTCCAACATGTTAGGACACTTCCAAGACACATCTAACTATTGTCTTTCAATATCTATCATTGGTAGGTTCTAAACTTTTTCTATATTTGTAAATATTTTAAGCAGTTTTGTCATTTATAGAATCCTTAATAACAACGACAACAATGTGTCAATGTACTGTGCCCTACTTTGCTAGGTTCTAGACCAACATATTCTTTGGGAGAGAAGGGACTCTAGAATTTGGTCTCCTGATCCTTTGCTAGGCTTCTCCTCTTGGTTCCTATTACATATTTTGTTTGTTGCTTTTCCGACCCAGATTAATTCTGCTTTTGTCGGAATTTGGAAGAGTAAAATTCCAAAGAAGTTCAGTTTTTTTCCTGTGACAGGGTTTACATGGTGATTATCATTTAACAACATAATTTTTATGTATTGAGAAAAGGACTATATCATCGGCCAGTTGTACGTGGTTTATGCTACGTGATTATTTACAGGTTCAAAACTGAGATTGATGGCAACAACTTGAGTCGAGCTACTAAGCTAAGGCAACCCACTAGGCCACTACCAAGTTTAAAAGGGAGGGAGATTTGGGATCTCCAGGCCTAAAACCTCTTGATCCAATGATCTTCTCTTCTTTTCTTATTATTACTTAGGCTAATTGAATGGTGGGGTTTTATTTATTTATTATTGTTTTCTTTATTTTTCTTTTCTTTGGGTAGTCCAACTGGCTTTTACTGATGTTGTCCTATCACTCGTGTATTTGTTTTCGATTGCTGTGAGCGTTTCTTATAAAAATGGAAAAAAAGTTTACAATGCAAATTGGTGCCTTTTTTTTCTTATTTCTTCCGTTACTTTACTTTACTGTTTTTATTGGTTTTATTTTTATTTCCATTGAGCATTAGTCTCTTTTTAATATATGAATTTTTTGTTTATTTATTTATGTTGTTCATTTTGAAAAACAAGAACTTATTGCTTCAATCTATATGAATGCGTGATTGAGTTGTTTGTCTTAGCATAGTTTGTGATGGCAGGCCTTTGCTGATGGTCGGTCATCATATAAACATTTAGGACAAGTTGCACTTAAGCAGAACATACGGAAAATTACAGTGGAGAGTCTACAACTTGAACCACTGTGCGATGAGGTTCTTGATTTTATATTTTTCTTCATTTTGATGATAATTATTGCTTTCCGGTCTATGTGAAAGCATATATGTATATTATTGTGTAGTTTTCTAAATATGTTATTTATAGGTGGTCATCAATTCTTTTTCCTTTATTGTAATCCTACATTTTGTTCTATTAGGAATACTTATTTTTCTTGAAATTAAGAATTTAAAATTATCCCAAAGTGCATTAAAACCTGTTAAAACACATTAAAAATAGGTATACTTTCATCTTTATTTTTAGGAATTTTTTTAACAACTTTATAATTTTTTTTCCCTATATGTTTGGAGTGCTAAGTTGTTTAACTAAGAATCGACTATTGAATCGTATAAGTTGATTTCTTTAAACAAATGAAATTTATTTTAAAATTTTTACTATAATTTTGTAAACTAAGCATGGATATAAATATTTCCTTTTCATATGCAATTCAATTGCTCTTATAATTGTGGAACATAGAGAAAATTATTATGTTTCTTGTTTCAGCTGGTTGATTATTTCGCGGAGCGATTGATATCCAAGCAAACATTGTTAAGAAATTCAGTTATGCAGAAAAGATCCAATAATCAGGTAAAAGGAGTTATGTTGAATTGAAGTAGTCATGGAGCATTGGCCTTCGGATGATAATCTCTTAGGGTTCGTTTGGTTTAAGATTTTTCTACCTAAGAATTAGCACATACATTTAAAACTCATGTTTGTTTCACAATTTTCTAACTTTTGCTGGGAATAACTGATTCCCAGGCATCCTTATTTACCACATACCAGGGTTTTTTATTCTCTTCCTAAACAGTGAGTTTCCTCTTTTTCCCCTCTATTTTTTTATTATTTTATTTGAAATACAGTTAACTAATTAATACTAACTTTAAAAAAAAAATTTGATAGGTGGAGCCAATGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAATTAATCTATTAAGTTTATATAAGAATTTTCTGATTAATTTCATAAATTAAATTCAAACATATTTTTTGTCCTTAAAATTCTAATAGCATTTTCTATGCTCAACCAAACACTGTCATCTTATTCCCAGGTATTCCATTCCTAGGCATCTATGAAACCTTAGACCAAAGGACTCCTTAATGGCATCTAAGCATGCTAAATGTATTGATAAAGTGTGCTTCATTAACTGTGGAAGAATGCTTCAATTTCATAAGATGCAGTTTGATCTTTTGTAAAATTTTGTTGTCTTATAGAGCTGCCTTGACTTATTATTATTTATTTATTAAAAGGAAACAAGACTCTTCATTGAGTGAAAATAAGTATTCTTAATATTCTGCCATTGAATTAGATAAGAAATATCGGGAATGTATTGCATTTTGAATCTTTGGAAAGGAAAACTAAAAATTAAAAAAGAAAAAAAAGGAAACCTAGGAAACAAGTTTACCTTTGACTTCTCTCTCTCTTTTGTGGATTAAGGGGGACGGTTTCTGTAAAGTTACAAGTTTTGACCTTTTTAATGATTCGTTGTGATGGAGCTTCATCATCTTAGTTGCTTTTGTCTTCTGCTTATTTTGTGCATTTGGGGGTGGAGAGGGGCAGATATCAAAGGGAAATTATGTAGTTTTAATTACAAAGCATATTTTGACAGCAAGAATGTTGAAACTTGAAAGCTGTGATTATTTTGGTGATATTGGAGCCATAACTCTTGCCTTGTATAAATTTTAACATGCTTACTGGAGGAAAATTTTAGCATGATTTGCTGCTTCTAATTGCTTACTTGTTTCTACAGATTGCTGTTGCATTTACATATTATCGAGGTGGAGCATTAATTAGTTGCAAGTATCGTGATGCCAAGAAAAGGTTCTGGCAGGTTCTTGGTTCAACTTATAAATAAAACTGTATGATAAATTTTTACTTATCCTAATCCTAAAGGCTTAAAATATATGCTTAGAGTAAATTCAGTTCATCTTAAATCATTTTGATTTTACCTTCTTCCCACTAGAAAAGTGTGCCGTCTGCTTTACTTGTATAATGTTATTGTAAGAAAATATATATAATCATCATCTGATTGTCTACCTTCCTTTATTTCAGGAGGCCAAAACTGAGAAAATATTTTATGGATTGGATGACATAGATGGTGCAAGTGATATCATCATAGTATGAGTTATCTAGTATGGAAAATCACTCCACTTAGATTTATTACTCATAATTTTCCTTTGTAATATAGGTTGAAGGGGAGATAGACAAGCTCTCAATGGCAGAAGCTGGTATCCATAATTGCGTGAGTGTTCCTGATGGTGCACCACCATCAGTTTCCAAAAAAAACGTACCTCCTGCACATCAGGTTACACTTTGA

mRNA sequence

ATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAGACCTCCAGCCCTTTTGGGGAATCCACCAGACAAGGCTTCAATCTCCACTCGATTGAATATTCTAAGAAAAAAATTGCAGGATCTTGACATTGATATCGAAGCTTGTGTTCCTGGGCAGTTTTCCAGTTTGCTTTGTCCAATGTGTAAAGGTGGTGATACAAAGGAACGATCCTTCTCCCTAAATATCTCAGAAGATGGGGAGGCTGCTGTTTGGAATTGTTTTCGTGGAAAATGTGGTTGGAAAGGCCATACTCTGGCCTTTGCTGATGGTCGGTCATCATATAAACATTTAGGACAAGTTGCACTTAAGCAGAACATACGGAAAATTACAGTGGAGAGTCTACAACTTGAACCACTGTGCGATGAGCTGGTTGATTATTTCGCGGAGCGATTGATATCCAAGCAAACATTGTTAAGAAATTCAGTTATGCAGAAAAGATCCAATAATCAGATTGCTGTTGCATTTACATATTATCGAGGTGGAGCATTAATTAGTTGCAAGTATCGTGATGCCAAGAAAAGGTTCTGGCAGGAGGCCAAAACTGAGAAAATATTTTATGGATTGGATGACATAGATGGTGCAAGTGATATCATCATAGTTGAAGGGGAGATAGACAAGCTCTCAATGGCAGAAGCTGGTATCCATAATTGCGTGAGTGTTCCTGATGGTGCACCACCATCAGTTTCCAAAAAAAACGTACCTCCTGCACATCAGGTTACACTTTGA

Coding sequence (CDS)

ATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAGACCTCCAGCCCTTTTGGGGAATCCACCAGACAAGGCTTCAATCTCCACTCGATTGAATATTCTAAGAAAAAAATTGCAGGATCTTGACATTGATATCGAAGCTTGTGTTCCTGGGCAGTTTTCCAGTTTGCTTTGTCCAATGTGTAAAGGTGGTGATACAAAGGAACGATCCTTCTCCCTAAATATCTCAGAAGATGGGGAGGCTGCTGTTTGGAATTGTTTTCGTGGAAAATGTGGTTGGAAAGGCCATACTCTGGCCTTTGCTGATGGTCGGTCATCATATAAACATTTAGGACAAGTTGCACTTAAGCAGAACATACGGAAAATTACAGTGGAGAGTCTACAACTTGAACCACTGTGCGATGAGCTGGTTGATTATTTCGCGGAGCGATTGATATCCAAGCAAACATTGTTAAGAAATTCAGTTATGCAGAAAAGATCCAATAATCAGATTGCTGTTGCATTTACATATTATCGAGGTGGAGCATTAATTAGTTGCAAGTATCGTGATGCCAAGAAAAGGTTCTGGCAGGAGGCCAAAACTGAGAAAATATTTTATGGATTGGATGACATAGATGGTGCAAGTGATATCATCATAGTTGAAGGGGAGATAGACAAGCTCTCAATGGCAGAAGCTGGTATCCATAATTGCGTGAGTGTTCCTGATGGTGCACCACCATCAGTTTCCAAAAAAAACGTACCTCCTGCACATCAGGTTACACTTTGA

Protein sequence

MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQVTL*
BLAST of Csa1G542410 vs. Swiss-Prot
Match: TWIH_ARATH (Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=At1g30680 PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 6.2e-68
Identity = 135/250 (54.00%), Postives = 169/250 (67.60%), Query Frame = 1

Query: 2   KPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLL 61
           + NG+SS+ S   VP P        DK  + +RL  LR+KL +  +D E C PGQ S L+
Sbjct: 77  RTNGLSSYNSIPRVPTPVDTEVEA-DKRVVLSRLVTLRRKLAEQGVDAENCPPGQHSGLI 136

Query: 62  CPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALKQ 121
           CP C+GG++ E+S SL I+ DG +A WNCFRGKCG KG   A   G +S   + +V    
Sbjct: 137 CPTCEGGNSGEKSLSLFIAPDGSSATWNCFRGKCGLKGGVRADG-GLASADPIEKVE--- 196

Query: 122 NIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALI 181
             RKITVE ++LEPLCDE+ DYFA R IS++TL RN VMQKR  ++I +AFTY++ G L+
Sbjct: 197 --RKITVEGIELEPLCDEIQDYFAARAISRKTLERNRVMQKRIGDEIVIAFTYWQRGELV 256

Query: 182 SCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGA 241
           SCKYR   K F+QE KT +I YGLDDI+  S++IIVEGEIDKL+M EAG  NCVSVPDGA
Sbjct: 257 SCKYRSLTKMFFQERKTRRILYGLDDIEKTSEVIIVEGEIDKLAMEEAGFLNCVSVPDGA 316

Query: 242 PPSVSKKNVP 252
           P  VS K +P
Sbjct: 317 PAKVSSKEIP 319

BLAST of Csa1G542410 vs. Swiss-Prot
Match: PRIH_ARATH (Primase homolog protein OS=Arabidopsis thaliana GN=At1g30660 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 4.9e-57
Identity = 120/225 (53.33%), Postives = 152/225 (67.56%), Query Frame = 1

Query: 28  KASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAV 87
           K  + ++L  L +KL +  ID + C PG  S L+CP C+ GD+ E+S +L I  DG +A 
Sbjct: 28  KKVVLSKLVTLMRKLSEQGIDAQNCPPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAK 87

Query: 88  WNCFRGKCGWKGHTLAFADGRSSYKH-LGQVALKQNIRKITVESLQLEPLCDELVDYFAE 147
           W C R KCG KG  +   DG+   K  +G+V      RKITVES++LEPLCDE+ D+FA 
Sbjct: 88  WTC-RRKCGLKG--VLQVDGKLVSKDPIGKVE-----RKITVESIKLEPLCDEIQDFFAA 147

Query: 148 RLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLD 207
           R IS +TL RN VMQKR +++I +AFTY++ G L+SCKYR   K+F QE  T KI YGLD
Sbjct: 148 RAISGKTLERNRVMQKRIDDEIVIAFTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLD 207

Query: 208 DIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP 252
           DI+  S+IIIVEGE DKL+M EAG  NCVSVPDGAP +VS K +P
Sbjct: 208 DIEETSEIIIVEGEPDKLAMEEAGFFNCVSVPDGAPETVSSKEIP 244

BLAST of Csa1G542410 vs. TrEMBL
Match: A0A0A0LVL8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G542410 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 3.0e-146
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 1

Query: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60
           MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL
Sbjct: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60

Query: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120
           LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK
Sbjct: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120

Query: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180
           QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL
Sbjct: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180

Query: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240
           ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG
Sbjct: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240

Query: 241 APPSVSKKNVPPAHQVTL 259
           APPSVSKKNVPPAHQVTL
Sbjct: 241 APPSVSKKNVPPAHQVTL 258

BLAST of Csa1G542410 vs. TrEMBL
Match: A0A0A0LYM8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G541890 PE=4 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 4.2e-132
Identity = 234/253 (92.49%), Postives = 243/253 (96.05%), Query Frame = 1

Query: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60
           MKPNGVSSFTSHANVPRPPALL NPPDKAS STRLNILRKKLQDLDIDIEACVPGQF SL
Sbjct: 81  MKPNGVSSFTSHANVPRPPALLENPPDKASSSTRLNILRKKLQDLDIDIEACVPGQFYSL 140

Query: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120
           LCPMCKGGD++ERSFSLNISEDG AAVWNCFRGKCGWKGHTLAF DGRSSYK LGQVALK
Sbjct: 141 LCPMCKGGDSEERSFSLNISEDGGAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALK 200

Query: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180
           QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRS+NQIAVAFTYYRGGAL
Sbjct: 201 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSDNQIAVAFTYYRGGAL 260

Query: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240
           ISCKYRDA K+FWQE  TE+IFYG+DDIDGASDIIIVEGE+DKLSMAEAGIHNCVSVPDG
Sbjct: 261 ISCKYRDANKKFWQEPNTERIFYGIDDIDGASDIIIVEGEMDKLSMAEAGIHNCVSVPDG 320

Query: 241 APPSVSKKNVPPA 254
           AP SVS+K+VPPA
Sbjct: 321 APASVSEKDVPPA 333

BLAST of Csa1G542410 vs. TrEMBL
Match: A0A059CFW7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01628 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 8.1e-83
Identity = 157/255 (61.57%), Postives = 190/255 (74.51%), Query Frame = 1

Query: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60
           +K NGV     H+++PRP  L G  P    I T L +L+ +L++L ID++ C+PGQ+ SL
Sbjct: 91  LKANGVPH-RPHSSIPRPVQLEG--PVGRGIETPLKVLKMRLEELGIDMQTCLPGQYHSL 150

Query: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120
           LCPMCKGG + ERS S+ +S DG +AVW CFRG CGWKG T A A  RSSY    Q +  
Sbjct: 151 LCPMCKGGRSMERSLSIFVSPDGGSAVWTCFRGSCGWKGSTRAAAKPRSSYATSNQTSKV 210

Query: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180
           + IR+I+ ESL LEPLC+ELV YFAERLIS +TL RN+VMQK+  NQI +AFTY R G L
Sbjct: 211 KTIREISEESLGLEPLCNELVAYFAERLISAETLRRNAVMQKKCENQIVIAFTYRREGKL 270

Query: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240
           +SCKYRD KK+FWQEA TEKIFYGLDDI G S+IIIVEGE+DKL+M EAG  NCVSVPDG
Sbjct: 271 VSCKYRDVKKKFWQEAHTEKIFYGLDDIKGKSEIIIVEGEMDKLAMEEAGFRNCVSVPDG 330

Query: 241 APPSVSKKNVPPAHQ 256
           APPS S K++PP  Q
Sbjct: 331 APPSTSDKDLPPEGQ 342

BLAST of Csa1G542410 vs. TrEMBL
Match: A0A059CGG0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01628 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 8.1e-83
Identity = 157/255 (61.57%), Postives = 190/255 (74.51%), Query Frame = 1

Query: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60
           +K NGV     H+++PRP  L G  P    I T L +L+ +L++L ID++ C+PGQ+ SL
Sbjct: 91  LKANGVPH-RPHSSIPRPVQLEG--PVGRGIETPLKVLKMRLEELGIDMQTCLPGQYHSL 150

Query: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120
           LCPMCKGG + ERS S+ +S DG +AVW CFRG CGWKG T A A  RSSY    Q +  
Sbjct: 151 LCPMCKGGRSMERSLSIFVSPDGGSAVWTCFRGSCGWKGSTRAAAKPRSSYATSNQTSKV 210

Query: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180
           + IR+I+ ESL LEPLC+ELV YFAERLIS +TL RN+VMQK+  NQI +AFTY R G L
Sbjct: 211 KTIREISEESLGLEPLCNELVAYFAERLISAETLRRNAVMQKKCENQIVIAFTYRREGKL 270

Query: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240
           +SCKYRD KK+FWQEA TEKIFYGLDDI G S+IIIVEGE+DKL+M EAG  NCVSVPDG
Sbjct: 271 VSCKYRDVKKKFWQEAHTEKIFYGLDDIKGKSEIIIVEGEMDKLAMEEAGFRNCVSVPDG 330

Query: 241 APPSVSKKNVPPAHQ 256
           APPS S K++PP  Q
Sbjct: 331 APPSTSDKDLPPEGQ 342

BLAST of Csa1G542410 vs. TrEMBL
Match: W9RPH9_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_026953 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 8.3e-80
Identity = 149/252 (59.13%), Postives = 195/252 (77.38%), Query Frame = 1

Query: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60
           +K NG SS  S A+ PR   +L +P +K +  ++  IL++KL+DL ++ +  VPGQF+ L
Sbjct: 49  IKTNGYSS-VSEASDPRA-VVLEDPEEKNA--SQFRILKQKLEDLGLECDISVPGQFNHL 108

Query: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120
           +CPMC GGD +ERS SL I +DG +A+W CFR KCGW+G T AFA+ + +Y+   ++A  
Sbjct: 109 ICPMCNGGDQEERSLSLFIEQDGSSALWVCFRAKCGWRGSTRAFAESKPAYERPNKIARI 168

Query: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180
           + IR+IT+E L LEP CDE+V YF+ER+ISK+T+ RN+VMQKR ++Q A+AFTY+R G L
Sbjct: 169 KKIREITIEDLGLEPPCDEIVAYFSERMISKETMQRNAVMQKRYDDQFAIAFTYWRNGNL 228

Query: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240
           ISCKYRD  K+FWQEA TEKIFYGLDDI  ASDIIIVEGE+DKL+M EAG  NCVSVPDG
Sbjct: 229 ISCKYRDINKKFWQEADTEKIFYGLDDIKEASDIIIVEGEMDKLAMEEAGFRNCVSVPDG 288

Query: 241 APPSVSKKNVPP 253
           APP VS+K++PP
Sbjct: 289 APPCVSEKDLPP 296

BLAST of Csa1G542410 vs. TAIR10
Match: AT1G30680.1 (AT1G30680.1 toprim domain-containing protein)

HSP 1 Score: 258.8 bits (660), Expect = 3.5e-69
Identity = 135/250 (54.00%), Postives = 169/250 (67.60%), Query Frame = 1

Query: 2   KPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLL 61
           + NG+SS+ S   VP P        DK  + +RL  LR+KL +  +D E C PGQ S L+
Sbjct: 77  RTNGLSSYNSIPRVPTPVDTEVEA-DKRVVLSRLVTLRRKLAEQGVDAENCPPGQHSGLI 136

Query: 62  CPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALKQ 121
           CP C+GG++ E+S SL I+ DG +A WNCFRGKCG KG   A   G +S   + +V    
Sbjct: 137 CPTCEGGNSGEKSLSLFIAPDGSSATWNCFRGKCGLKGGVRADG-GLASADPIEKVE--- 196

Query: 122 NIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALI 181
             RKITVE ++LEPLCDE+ DYFA R IS++TL RN VMQKR  ++I +AFTY++ G L+
Sbjct: 197 --RKITVEGIELEPLCDEIQDYFAARAISRKTLERNRVMQKRIGDEIVIAFTYWQRGELV 256

Query: 182 SCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGA 241
           SCKYR   K F+QE KT +I YGLDDI+  S++IIVEGEIDKL+M EAG  NCVSVPDGA
Sbjct: 257 SCKYRSLTKMFFQERKTRRILYGLDDIEKTSEVIIVEGEIDKLAMEEAGFLNCVSVPDGA 316

Query: 242 PPSVSKKNVP 252
           P  VS K +P
Sbjct: 317 PAKVSSKEIP 319

BLAST of Csa1G542410 vs. TAIR10
Match: AT1G30660.1 (AT1G30660.1 nucleic acid binding;nucleic acid binding)

HSP 1 Score: 222.6 bits (566), Expect = 2.8e-58
Identity = 120/225 (53.33%), Postives = 152/225 (67.56%), Query Frame = 1

Query: 28  KASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAV 87
           K  + ++L  L +KL +  ID + C PG  S L+CP C+ GD+ E+S +L I  DG +A 
Sbjct: 28  KKVVLSKLVTLMRKLSEQGIDAQNCPPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAK 87

Query: 88  WNCFRGKCGWKGHTLAFADGRSSYKH-LGQVALKQNIRKITVESLQLEPLCDELVDYFAE 147
           W C R KCG KG  +   DG+   K  +G+V      RKITVES++LEPLCDE+ D+FA 
Sbjct: 88  WTC-RRKCGLKG--VLQVDGKLVSKDPIGKVE-----RKITVESIKLEPLCDEIQDFFAA 147

Query: 148 RLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLD 207
           R IS +TL RN VMQKR +++I +AFTY++ G L+SCKYR   K+F QE  T KI YGLD
Sbjct: 148 RAISGKTLERNRVMQKRIDDEIVIAFTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLD 207

Query: 208 DIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP 252
           DI+  S+IIIVEGE DKL+M EAG  NCVSVPDGAP +VS K +P
Sbjct: 208 DIEETSEIIIVEGEPDKLAMEEAGFFNCVSVPDGAPETVSSKEIP 244

BLAST of Csa1G542410 vs. NCBI nr
Match: gi|700210854|gb|KGN65950.1| (hypothetical protein Csa_1G542410 [Cucumis sativus])

HSP 1 Score: 525.8 bits (1353), Expect = 4.3e-146
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 1

Query: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60
           MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL
Sbjct: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60

Query: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120
           LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK
Sbjct: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120

Query: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180
           QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL
Sbjct: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180

Query: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240
           ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG
Sbjct: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240

Query: 241 APPSVSKKNVPPAHQVTL 259
           APPSVSKKNVPPAHQVTL
Sbjct: 241 APPSVSKKNVPPAHQVTL 258

BLAST of Csa1G542410 vs. NCBI nr
Match: gi|778662022|ref|XP_011659237.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial-like isoform X1 [Cucumis sativus])

HSP 1 Score: 520.8 bits (1340), Expect = 1.4e-144
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 1

Query: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60
           MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL
Sbjct: 78  MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 137

Query: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120
           LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK
Sbjct: 138 LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 197

Query: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180
           QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL
Sbjct: 198 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 257

Query: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240
           ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG
Sbjct: 258 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 317

Query: 241 APPSVSKKNVPPAHQ 256
           APPSVSKKNVPPAHQ
Sbjct: 318 APPSVSKKNVPPAHQ 332

BLAST of Csa1G542410 vs. NCBI nr
Match: gi|778662024|ref|XP_011659240.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial-like isoform X2 [Cucumis sativus])

HSP 1 Score: 520.8 bits (1340), Expect = 1.4e-144
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 1

Query: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60
           MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL
Sbjct: 78  MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 137

Query: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120
           LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK
Sbjct: 138 LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 197

Query: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180
           QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL
Sbjct: 198 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 257

Query: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240
           ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG
Sbjct: 258 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 317

Query: 241 APPSVSKKNVPPAHQ 256
           APPSVSKKNVPPAHQ
Sbjct: 318 APPSVSKKNVPPAHQ 332

BLAST of Csa1G542410 vs. NCBI nr
Match: gi|778662016|ref|XP_011659233.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial [Cucumis sativus])

HSP 1 Score: 478.8 bits (1231), Expect = 6.0e-132
Identity = 234/253 (92.49%), Postives = 243/253 (96.05%), Query Frame = 1

Query: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60
           MKPNGVSSFTSHANVPRPPALL NPPDKAS STRLNILRKKLQDLDIDIEACVPGQF SL
Sbjct: 81  MKPNGVSSFTSHANVPRPPALLENPPDKASSSTRLNILRKKLQDLDIDIEACVPGQFYSL 140

Query: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120
           LCPMCKGGD++ERSFSLNISEDG AAVWNCFRGKCGWKGHTLAF DGRSSYK LGQVALK
Sbjct: 141 LCPMCKGGDSEERSFSLNISEDGGAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALK 200

Query: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180
           QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRS+NQIAVAFTYYRGGAL
Sbjct: 201 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSDNQIAVAFTYYRGGAL 260

Query: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240
           ISCKYRDA K+FWQE  TE+IFYG+DDIDGASDIIIVEGE+DKLSMAEAGIHNCVSVPDG
Sbjct: 261 ISCKYRDANKKFWQEPNTERIFYGIDDIDGASDIIIVEGEMDKLSMAEAGIHNCVSVPDG 320

Query: 241 APPSVSKKNVPPA 254
           AP SVS+K+VPPA
Sbjct: 321 APASVSEKDVPPA 333

BLAST of Csa1G542410 vs. NCBI nr
Match: gi|659068706|ref|XP_008445781.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 435.3 bits (1118), Expect = 7.7e-119
Identity = 216/264 (81.82%), Postives = 232/264 (87.88%), Query Frame = 1

Query: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60
           MKPNG SSFTSHANVPRPPA   NP DKA  ST LNILRKKLQDL IDIEACVPGQFS L
Sbjct: 84  MKPNGFSSFTSHANVPRPPAFSENPRDKALSSTLLNILRKKLQDLGIDIEACVPGQFSGL 143

Query: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLG----- 120
           LCPMCKGGD+KERSF+L+ISEDG AAVWNCFRGKCGWKGHTLA A+ RSSY+ LG     
Sbjct: 144 LCPMCKGGDSKERSFALHISEDGGAAVWNCFRGKCGWKGHTLALAEDRSSYQDLGRVALK 203

Query: 121 ----QVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVA 180
               +VALKQNIRKITVESLQLEPLCD++V YFAERLISK+TLLRNSVMQK   +QIA+A
Sbjct: 204 QDLGRVALKQNIRKITVESLQLEPLCDQVVAYFAERLISKETLLRNSVMQKTFGDQIAIA 263

Query: 181 FTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGI 240
           FTYYRGGAL+SCKYRD  K+FWQEA TEKIFYG+DDI+GASDIIIVEGEIDKLSMAEAGI
Sbjct: 264 FTYYRGGALVSCKYRDVNKKFWQEANTEKIFYGIDDIEGASDIIIVEGEIDKLSMAEAGI 323

Query: 241 HNCVSVPDGAPPSVSKKNVPPAHQ 256
           HNCVSVPDGAPPSVSKK+VP A Q
Sbjct: 324 HNCVSVPDGAPPSVSKKDVPSADQ 347

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TWIH_ARATH6.2e-6854.00Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=... [more]
PRIH_ARATH4.9e-5753.33Primase homolog protein OS=Arabidopsis thaliana GN=At1g30660 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LVL8_CUCSA3.0e-146100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G542410 PE=4 SV=1[more]
A0A0A0LYM8_CUCSA4.2e-13292.49Uncharacterized protein OS=Cucumis sativus GN=Csa_1G541890 PE=4 SV=1[more]
A0A059CFW7_EUCGR8.1e-8361.57Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01628 PE=4 SV=1[more]
A0A059CGG0_EUCGR8.1e-8361.57Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01628 PE=4 SV=1[more]
W9RPH9_9ROSA8.3e-8059.13Uncharacterized protein OS=Morus notabilis GN=L484_026953 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G30680.13.5e-6954.00 toprim domain-containing protein[more]
AT1G30660.12.8e-5853.33 nucleic acid binding;nucleic acid binding[more]
Match NameE-valueIdentityDescription
gi|700210854|gb|KGN65950.1|4.3e-146100.00hypothetical protein Csa_1G542410 [Cucumis sativus][more]
gi|778662022|ref|XP_011659237.1|1.4e-144100.00PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial-like isoform X1 ... [more]
gi|778662024|ref|XP_011659240.1|1.4e-144100.00PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial-like isoform X2 ... [more]
gi|778662016|ref|XP_011659233.1|6.0e-13292.49PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial [Cucumis sativus... [more]
gi|659068706|ref|XP_008445781.1|7.7e-11981.82PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial isoform X1 [Cucu... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006171TOPRIM_domain
IPR027032Twinkle-like protein
Vocabulary: Molecular Function
TermDefinition
GO:0003697single-stranded DNA binding
GO:00431395'-3' DNA helicase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032508 DNA duplex unwinding
biological_process GO:0006260 DNA replication
cellular_component GO:0005657 replication fork
molecular_function GO:0043139 5'-3' DNA helicase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003697 single-stranded DNA binding
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU158813cucumber EST collection version 3.0transcribed_cluster
CU170773cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa1G542410.1Csa1G542410.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU158813CU158813transcribed_cluster
CU170773CU170773transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006171Toprim domainPFAMPF13662Toprim_4coord: 213..246
score: 4.
IPR027032Twinkle-like proteinPANTHERPTHR12873T7-LIKE MITOCHONDRIAL DNA HELICASEcoord: 32..246
score: 4.7
NoneNo IPR availableGENE3DG3DSA:3.40.1360.10coord: 199..246
score: 9.7
NoneNo IPR availablePANTHERPTHR12873:SF2SUBFAMILY NOT NAMEDcoord: 32..246
score: 4.7
NoneNo IPR availableunknownSSF56731DNA primase corecoord: 144..243
score: 1.31

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Csa1G542410Cla019748Watermelon (97103) v1cuwmB014
The following gene(s) are paralogous to this gene:

None