Csa1G528520 (gene) Cucumber (Chinese Long) v2

NameCsa1G528520
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionCysteine proteinase; contains IPR013128 (Peptidase C1A, papain)
LocationChr1 : 18526310 .. 18527696 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAAATTTCTTATTGTTTTCCTTGTTTTGATTGCTTTCATCTCTCACATATGTGAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTATAAAAGATGGAGTAGCCACCATAGAATCTCAAGGAATGAACATGAGATGGACAGACGTTTCAAGGTCTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAGTTTGCTGATATGTCTGATGATGAGTTCAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGTTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGGAAAAAAGGAGCAAGGCGGATGTGGTAATTAATTTAATTTAATTTATAATTTTTTCAAAATCAAGGTGCATGTGGTAATTAATTAAATTTAATTTATAATTTTTCTAAAATCAAGGCGGATGTGGTAATTAATTAAATTTAATTTATAATTTTTCTAAAGTCTAGTGCCTTATAACTAATAATTATGTGTTCGACAGGAAGCTGCTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAGAACAAATGAGTTGGTATCTCTGTCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATATCTCTGCATTTGAGTTCATAATGGAAAATGGTGGAATCACCGTTGAGAATAACTATCCATATTATGCCGGAGATGGATATTGTCGTAGACGAGGAGTGAGTTTAAACCAATATAATTCTAATTAAACTATATAAATTTATTATATAAGTGAGTTATTGATCATAATATAATTATGTGTATTGTGTTTGATATATACTTCACTAATAGCCTAATAACGAGAGAGTGACAATTGATGGATATGAGAATGTACCTCGAAACAACGAGTACGCTTTGATGAAAGCAGTTGCACACCAACCGGTAGCAGTGTCTATAGCGTCAAGGGGAAGTGACTTTAAATTTTATGGAGAGGCAAGTGTAGTTAATTTTGTTGTTATAATTTAATTATAATTTGGTGCAACATAATTAAAAGAAGAAATTGATAATTATTTGCAGGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGTATCCTGCCTTTCCGGTCAAGTATTGA

mRNA sequence

ATGATGAAATTTCTTATTGTTTTCCTTGTTTTGATTGCTTTCATCTCTCACATATGTGAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTATAAAAGATGGAGTAGCCACCATAGAATCTCAAGGAATGAACATGAGATGGACAGACGTTTCAAGGTCTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAGTTTGCTGATATGTCTGATGATGAGTTCAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGTTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGGAAAAAAGGAGCAAGGCGGATGTGCTGCTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAGAACAAATGAGTTGGTATCTCTGTCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATATCTCTGCATTTGAGTTCATAATGGAAAATGGTGGAATCACCGTTGAGAATAACTATCCATATTATGCCGGAGATGGATATTGTCGTAGACGAGGACCTAATAACGAGAGAGTGACAATTGATGGATATGAGAATGTACCTCGAAACAACGAGTACGCTTTGATGAAAGCAGTTGCACACCAACCGGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGTATCCTGCCTTTCCGGTCAAGTATTGA

Coding sequence (CDS)

ATGATGAAATTTCTTATTGTTTTCCTTGTTTTGATTGCTTTCATCTCTCACATATGTGAGAGCTTTGAGCTAGAAAGAAAGGATTTTGAATCTGAAAAAAGTTTGATGCAACTCTATAAAAGATGGAGTAGCCACCATAGAATCTCAAGGAATGAACATGAGATGGACAGACGTTTCAAGGTCTTCAAAGATAATGCAAAACATGTGTTCAAAGTGAACCACATGGGAAAATCATTAAAATTGAAGCTTAACCAGTTTGCTGATATGTCTGATGATGAGTTCAGTAAGACATATGGTTCCAACATTACTTACTACAAAAACTTACATGCCAAGGTTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAACAAATATTCCATCTTCAATCGACTGGAGGAAAAAAGGAGCAAGGCGGATGTGCTGCTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAGAACAAATGAGTTGGTATCTCTGTCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAGATTATATCTCTGCATTTGAGTTCATAATGGAAAATGGTGGAATCACCGTTGAGAATAACTATCCATATTATGCCGGAGATGGATATTGTCGTAGACGAGGACCTAATAACGAGAGAGTGACAATTGATGGATATGAGAATGTACCTCGAAACAACGAGTACGCTTTGATGAAAGCAGTTGCACACCAACCGGGAATGTTTACGGAAGAGAACTTTTGCGGAATTAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGTATCCTGCCTTTCCGGTCAAGTATTGA

Protein sequence

MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFKVFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFMYERATNIPSSIDWRKKGARRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENVPRNNEYALMKAVAHQPGMFTEENFCGIRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY*
BLAST of Csa1G528520 vs. Swiss-Prot
Match: CYSEP_RICCO (Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.7e-83
Identity = 166/347 (47.84%), Postives = 218/347 (62.82%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           M KF+++ L L A +  I ESF+   K+ ESE+SL  LY+RW SHH +SR+ HE  +RF 
Sbjct: 1   MQKFILLALSL-ALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFN 60

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKVGGRVGGF 120
           VFK NA HV   N M K  KLKLN+FADM++ EF  TY GS + +++       G  G F
Sbjct: 61  VFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPRGN-GTF 120

Query: 121 MYERATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQEV 180
           MYE+   +P+S+DWRKKGA      +  C  CWAF+ + AVE I+QI+TN+LVSLSEQE+
Sbjct: 121 MYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQEL 180

Query: 181 VDCDYKVG-GCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYE 240
           VDCD     GC GG    AFEFI + GGIT E NYPY A DG C     N   V+IDG+E
Sbjct: 181 VDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHE 240

Query: 241 NVPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSD 300
           NVP N+E AL+KAVA+QP                 G+FT    CG  +DH V +VGYG+ 
Sbjct: 241 NVPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFT--GSCGTELDHGVAIVGYGTT 300

Query: 301 EEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVK 320
            +G  YW ++N +G +WG  GY++M+RG  + +G+CG+AM  ++P+K
Sbjct: 301 IDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

BLAST of Csa1G528520 vs. Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.4e-82
Identity = 161/346 (46.53%), Postives = 219/346 (63.29%), Query Frame = 1

Query: 2   MKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFKV 61
           MK L+  ++ ++ +  +  SF+   KD ESE+SL  LY+RW SHH +SR+  E  +RF V
Sbjct: 3   MKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRFNV 62

Query: 62  FKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKVGGRVGGFM 121
           FK N  HV   N M K  KLKLN+FADM++ EF  TY GS + ++K       G  G FM
Sbjct: 63  FKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGS-GTFM 122

Query: 122 YERATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQEVV 181
           YE+  ++P+S+DWRKKGA      +  C  CWAF+ + AVE I+QI+TN+LVSLSEQE+V
Sbjct: 123 YEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELV 182

Query: 182 DCDYKVG-GCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYEN 241
           DCD +   GC GG   SAFEFI + GGIT E+NYPY A +G C     N+  V+IDG+EN
Sbjct: 183 DCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGHEN 242

Query: 242 VPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSDE 301
           VP N+E AL+KAVA+QP                 G+FT +  C   ++H V +VGYG+  
Sbjct: 243 VPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGD--CNTDLNHGVAIVGYGTTV 302

Query: 302 EG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVK 320
           +G +YWI+RN +G +WG  GY++MQR     +G+CG+AM  ++P+K
Sbjct: 303 DGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

BLAST of Csa1G528520 vs. Swiss-Prot
Match: CYSEP_PHAVU (Vignain OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 300.8 bits (769), Expect = 1.8e-80
Identity = 156/344 (45.35%), Postives = 213/344 (61.92%), Query Frame = 1

Query: 3   KFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFKVF 62
           K L+  ++  + +  +  SF+   KD  SE+SL  LY+RW SHH +SR+  E  +RF VF
Sbjct: 4   KKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVF 63

Query: 63  KDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFMYE 122
           K N  HV   N M K  KLKLN+FADM++ EF  TY  +   +  +        G FMYE
Sbjct: 64  KANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGAFMYE 123

Query: 123 RATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQEVVDC 182
           +  ++P S+DWRKKGA      +  C  CWAF+ V AVE I+QI+TN+LV+LSEQE+VDC
Sbjct: 124 KVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVDC 183

Query: 183 DYKVG-GCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENVP 242
           D +   GC GG   SAFEFI + GGIT E+NYPY A +G C     N+  V+IDG+ENVP
Sbjct: 184 DKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENVP 243

Query: 243 RNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSDEEG 302
            N+E AL+KAVA+QP                 G+FT +  C   ++H V +VGYG+  +G
Sbjct: 244 ANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGD--CSTDLNHGVAIVGYGTTVDG 303

Query: 303 -DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVK 320
            +YWI+RN +G +WG +GY++MQR     +G+CG+AM P++P+K
Sbjct: 304 TNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

BLAST of Csa1G528520 vs. Swiss-Prot
Match: CEP3_ARATH (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana GN=CEP3 PE=2 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 3.9e-80
Identity = 159/345 (46.09%), Postives = 219/345 (63.48%), Query Frame = 1

Query: 6   IVFLVLIAFISHICES--FELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFKVFK 65
           + F+VLI+F+S +  S  F+ + K+ E+E+++ +LY+RW  HH +SR  HE  +RF VF+
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 66  DNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKVGGRVGGFMYE 125
            N  HV + N   K  KLK+N+FAD++  EF  +Y GSN+ +++ L     G  GGFMYE
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS-GGFMYE 122

Query: 126 RATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQEVVDC 185
             T +PSS+DWR+KGA      ++ C  CWAF+ VAAVE I++IRTN+LVSLSEQE+VDC
Sbjct: 123 NVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDC 182

Query: 186 DYKVG-GCRGGDYISAFEFIMENGGITVENNYPYYAGD-GYCRRRGPNNERVTIDGYENV 245
           D +   GC GG    AFEFI  NGGI  E  YPY + D  +CR      E VTIDG+E+V
Sbjct: 183 DTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHV 242

Query: 246 PRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSDEE 305
           P N+E  L+KAVAHQP                 G+F  E  CG +++H VV+VGYG  + 
Sbjct: 243 PENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGE--CGTQLNHGVVIVGYGETKN 302

Query: 306 G-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVK 320
           G  YWI+RN +G +WG  GY++++RG    +G CG+AM  ++P K
Sbjct: 303 GTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of Csa1G528520 vs. Swiss-Prot
Match: CEP2_ARATH (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.3e-79
Identity = 158/347 (45.53%), Postives = 214/347 (61.67%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           M K L++FL  +  +   C  F+ + K+ ESE+ L  LY RW SHH + R+ +E ++RF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHA-KVGGRVGG 120
           VF+ N  HV   N   +S KLKLN+FAD++ +EF   Y GSNI +++ L   K G +   
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 121 FMYERATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQE 180
           + +E  + +PSS+DWRKKGA      +  C  CWAF+ VAAVE I++I+TN+LVSLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 181 VVDCDYKVG-GCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGY 240
           +VDCD K   GC GG    AFEFI +NGGIT E++YPY   DG C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 241 ENVPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGS 300
           E+VP N+E AL+KAVA+QP                 G+FT    CG  ++H V  VGYGS
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFT--GSCGTELNHGVAAVGYGS 300

Query: 301 DEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVK 320
           +    YWI+RN +G +WG  GY+K++R    P+G CG+AM  ++P+K
Sbjct: 301 ERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of Csa1G528520 vs. TrEMBL
Match: A0A0A0LY73_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528520 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 1.4e-190
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK
Sbjct: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120
           VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM
Sbjct: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120

Query: 121 YERATNIPSSIDWRKKGARRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCDYKVGG 180
           YERATNIPSSIDWRKKGARRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCDYKVGG
Sbjct: 121 YERATNIPSSIDWRKKGARRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCDYKVGG 180

Query: 181 CRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENVPRNNEYAL 240
           CRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENVPRNNEYAL
Sbjct: 181 CRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENVPRNNEYAL 240

Query: 241 MKAVAHQPGMFTEENFCGIRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRG 300
           MKAVAHQPGMFTEENFCGIRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRG
Sbjct: 241 MKAVAHQPGMFTEENFCGIRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRG 300

Query: 301 TRNPQGVCGMAMYPAFPVKY 321
           TRNPQGVCGMAMYPAFPVKY
Sbjct: 301 TRNPQGVCGMAMYPAFPVKY 320

BLAST of Csa1G528520 vs. TrEMBL
Match: A0A0A0KGB1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 2.8e-149
Identity = 264/349 (75.64%), Postives = 288/349 (82.52%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           +MKFLIV LVL+AF  +ICESFELERKDFESEKSLMQLYKRWSSHHRISRN +EM  RFK
Sbjct: 3   VMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNRFK 62

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAK----VGGRV 120
           VFK+NAKHVFKVN MGKSLKLKLNQFADMSDDEF   Y SNITYYK+LHAK     GGR+
Sbjct: 63  VFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGGRI 122

Query: 121 GGFMYERATNIPSSIDWRKKGA--------RRMCCWAFAAVAAVESIHQIRTNELVSLSE 180
           GGFMYE A NIPSSIDWRKKGA        R   CWAFAAVAAVESIHQI+TNELVSLSE
Sbjct: 123 GGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSE 182

Query: 181 QEVVDCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDG 240
           +EV+DCDY+ GGCRGG Y SAFEF+M+N G+T+E+NYPYY G+GYCRRRG  N+RV IDG
Sbjct: 183 EEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRIDG 242

Query: 241 YENVPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYG 300
           YENVPRNNEYALMKAVAHQP                 GMFTE +FCG  IDHTVVVVGYG
Sbjct: 243 YENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHTVVVVGYG 302

Query: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 321
           +DE+GDYWIIRNQYG +WGMNGYMKMQRG  +PQGVCGMAM PA+PVKY
Sbjct: 303 TDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVKY 351

BLAST of Csa1G528520 vs. TrEMBL
Match: A0A0A0KW34_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G013580 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 9.0e-100
Identity = 181/226 (80.09%), Postives = 198/226 (87.61%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           MMKFLIV +VLIAF +H+CESFELERKDFESEKSLMQLYKRWSSHHRISRN HEM +RFK
Sbjct: 3   MMKFLIVLVVLIAFTTHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFK 62

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120
           +F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y  LHA    RVG FM
Sbjct: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHA---NRVGEFM 122

Query: 121 YERATNIPSSIDWRKKGARRM--------CCWAFAAVAAVESIHQIRTNELVSLSEQEVV 180
           YERA NIPSSIDWR+KGA            CWAFAAVAAVESIHQI+TNELVSLSEQEVV
Sbjct: 123 YERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 182

Query: 181 DCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRG 219
           DCDYKVGGCRGG+Y SAFEFIM+NGGIT+E NYPY+AG+GYCRRRG
Sbjct: 183 DCDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRG 225

BLAST of Csa1G528520 vs. TrEMBL
Match: A0A0A0LMU4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.5e-89
Identity = 171/345 (49.57%), Postives = 234/345 (67.83%), Query Frame = 1

Query: 3   KFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFKVF 62
           KFL+V L+LI  +S + ESFE + K+  +E+SL QLY+RW  HH ISRN  E  +RF VF
Sbjct: 5   KFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKRFSVF 64

Query: 63  KDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYG-SNITYYKNLHAKVGGRVGGFMY 122
           K+N  HVF VN M K  KLKLN+FADMS+ EF   Y  SNI++Y+ LH +  G  GGFMY
Sbjct: 65  KENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRRG-AGGFMY 124

Query: 123 ERATNIPSSIDWRKKGA--------RRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVVD 182
           E+ T++PSS+DWR++GA        R   CWAF++VAAVE I++I+TN+L+SLSEQE++D
Sbjct: 125 EQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQELLD 184

Query: 183 CDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENVP 242
           C+Y+  GC GG    AF+FI  NGGI  EN+YPY+   G CR    ++  V IDGYE+VP
Sbjct: 185 CNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGYESVP 244

Query: 243 RNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSDEEG 302
             NE ALM+AVA+QP                 G+F  + +CG  ++H VV +GYG+ E+G
Sbjct: 245 -ENEDALMQAVANQPVSVAIDAAGRDFQFYSQGVF--DGYCGTELNHGVVAIGYGTTEDG 304

Query: 303 -DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 321
            DYW++RN +G  WG +GY++M+RG    +G+CG+AM  ++P+KY
Sbjct: 305 TDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

BLAST of Csa1G528520 vs. TrEMBL
Match: V4LTA3_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10010486mg PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.0e-82
Identity = 169/347 (48.70%), Postives = 223/347 (64.27%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           M K L++FL     +  +   F+   K+ ESE+SL +LY+RW SHH + R+ HE ++RF 
Sbjct: 1   MKKLLLIFLSSFLILQTV-RGFDYHDKELESEESLSKLYERWRSHHSVPRSLHEREKRFN 60

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKVGGRVGGF 120
           VF+ N  HV K N   K  KLKLN+FAD++  EF   Y GSNI +++ L     G    F
Sbjct: 61  VFRHNVMHVHKTNKKNKPYKLKLNKFADLTVHEFKNAYTGSNIKHHRMLQGLKRGS-KRF 120

Query: 121 MYERATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQEV 180
           MYE +T +PSS+DWR+KGA      +  C  CWAF+ VAAVE I++I+TN+LVSLSEQE+
Sbjct: 121 MYENSTRVPSSVDWREKGAVTEVKNQGQCGSCWAFSTVAAVEGINKIKTNKLVSLSEQEL 180

Query: 181 VDCD-YKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYE 240
           VDCD Y+  GC GG    AFEFI +NGGIT E+NYPY   DG C     N E VTIDG+E
Sbjct: 181 VDCDTYQNEGCNGGLMEIAFEFIKKNGGITTEDNYPYEGIDGKCDASKDNGEVVTIDGHE 240

Query: 241 NVPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSD 300
           NVP N+E AL+KAVA+QP                 G+FT   +CG  ++H V VVGYGSD
Sbjct: 241 NVPENDEDALLKAVANQPVSVAIDAGSSNFQFYSEGVFT--GYCGTELNHGVAVVGYGSD 300

Query: 301 EEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVK 320
            +G  YWI+RN +G +WG  GY+K++RG  + +G CG+AM  ++PVK
Sbjct: 301 SKGRKYWIVRNSWGPEWGEGGYIKLERGIDDSEGRCGIAMEASYPVK 343

BLAST of Csa1G528520 vs. TAIR10
Match: AT3G48350.1 (AT3G48350.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 299.7 bits (766), Expect = 2.2e-81
Identity = 159/345 (46.09%), Postives = 219/345 (63.48%), Query Frame = 1

Query: 6   IVFLVLIAFISHICES--FELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFKVFK 65
           + F+VLI+F+S +  S  F+ + K+ E+E+++ +LY+RW  HH +SR  HE  +RF VF+
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 66  DNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKVGGRVGGFMYE 125
            N  HV + N   K  KLK+N+FAD++  EF  +Y GSN+ +++ L     G  GGFMYE
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS-GGFMYE 122

Query: 126 RATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQEVVDC 185
             T +PSS+DWR+KGA      ++ C  CWAF+ VAAVE I++IRTN+LVSLSEQE+VDC
Sbjct: 123 NVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDC 182

Query: 186 DYKVG-GCRGGDYISAFEFIMENGGITVENNYPYYAGD-GYCRRRGPNNERVTIDGYENV 245
           D +   GC GG    AFEFI  NGGI  E  YPY + D  +CR      E VTIDG+E+V
Sbjct: 183 DTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHV 242

Query: 246 PRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSDEE 305
           P N+E  L+KAVAHQP                 G+F  E  CG +++H VV+VGYG  + 
Sbjct: 243 PENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGE--CGTQLNHGVVIVGYGETKN 302

Query: 306 G-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVK 320
           G  YWI+RN +G +WG  GY++++RG    +G CG+AM  ++P K
Sbjct: 303 GTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of Csa1G528520 vs. TAIR10
Match: AT3G48340.1 (AT3G48340.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 296.2 bits (757), Expect = 2.4e-80
Identity = 158/347 (45.53%), Postives = 214/347 (61.67%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           M K L++FL  +  +   C  F+ + K+ ESE+ L  LY RW SHH + R+ +E ++RF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHA-KVGGRVGG 120
           VF+ N  HV   N   +S KLKLN+FAD++ +EF   Y GSNI +++ L   K G +   
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 121 FMYERATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQE 180
           + +E  + +PSS+DWRKKGA      +  C  CWAF+ VAAVE I++I+TN+LVSLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 181 VVDCDYKVG-GCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGY 240
           +VDCD K   GC GG    AFEFI +NGGIT E++YPY   DG C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 241 ENVPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGS 300
           E+VP N+E AL+KAVA+QP                 G+FT    CG  ++H V  VGYGS
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFT--GSCGTELNHGVAAVGYGS 300

Query: 301 DEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVK 320
           +    YWI+RN +G +WG  GY+K++R    P+G CG+AM  ++P+K
Sbjct: 301 ERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of Csa1G528520 vs. TAIR10
Match: AT5G50260.1 (AT5G50260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 287.7 bits (735), Expect = 8.6e-78
Identity = 155/346 (44.80%), Postives = 208/346 (60.12%), Query Frame = 1

Query: 2   MKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFKV 61
           MK  IV  + +  +    +  +   KD ESE SL +LY+RW SHH ++R+  E  +RF V
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 62  FKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTY-GSNITYYKNLHAKVGGRVGGFM 121
           FK N KH+ + N   KS KLKLN+F DM+ +EF +TY GSNI +++    +       FM
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKA-TKSFM 120

Query: 122 YERATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQEVV 181
           Y     +P+S+DWRK GA      +  C  CWAF+ V AVE I+QIRT +L SLSEQE+V
Sbjct: 121 YANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELV 180

Query: 182 DCDYKVG-GCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYEN 241
           DCD     GC GG    AFEFI E GG+T E  YPY A D  C     N   V+IDG+E+
Sbjct: 181 DCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHED 240

Query: 242 VPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSDE 301
           VP+N+E  LMKAVA+QP                 G+FT    CG  ++H V VVGYG+  
Sbjct: 241 VPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGR--CGTELNHGVAVVGYGTTI 300

Query: 302 EG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVK 320
           +G  YWI++N +G +WG  GY++MQRG R+ +G+CG+AM  ++P+K
Sbjct: 301 DGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

BLAST of Csa1G528520 vs. TAIR10
Match: AT3G19400.1 (AT3G19400.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 244.2 bits (622), Expect = 1.1e-64
Identity = 148/344 (43.02%), Postives = 200/344 (58.14%), Query Frame = 1

Query: 5   LIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNE-HEMDRRFKVFK 64
           +I+ ++L++    +    E+ER   E+E  LM  Y++W   +R + N   E +RRFK+FK
Sbjct: 14  VILSVLLLSSSLGVATETEIERN--ETEVRLM--YEQWLVENRKNYNGLGEKERRFKIFK 73

Query: 65  DNAKHVFKVNHM-GKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFMYE 124
           DN K V + N +  ++ ++ L +FAD++++EF   Y       K    K   +   ++Y+
Sbjct: 74  DNLKFVDEHNSVPDRTFEVGLTRFADLTNEEFRAIYLRK----KMERTKDSVKTERYLYK 133

Query: 125 RATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQEVVDC 184
               +P  +DWR  GA      +  C  CWAF+AV AVE I+QI T EL+SLSEQE+VDC
Sbjct: 134 EGDVLPDEVDWRANGAVVSVKDQGNCGSCWAFSAVGAVEGINQITTGELISLSEQELVDC 193

Query: 185 D--YKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGD-GYCRRRGPNNERV-TIDGYE 244
           D  +   GC GG    AFEFIM+NGGI  + +YPY A D G C     NN RV TIDGYE
Sbjct: 194 DRGFVNAGCDGGIMNYAFEFIMKNGGIETDQDYPYNANDLGLCNADKNNNTRVVTIDGYE 253

Query: 245 NVPRNNEYALMKAVAHQPGMFTEE---------------NFCGIRIDHTVVVVGYGSDEE 304
           +VPR++E +L KAVAHQP     E                 CGI +DH VVVVGYGS   
Sbjct: 254 DVPRDDEKSLKKAVAHQPVSVAIEASSQAFQLYKSGVMTGTCGISLDHGVVVVGYGSTSG 313

Query: 305 GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVK 320
            DYWIIRN +G  WG +GY+K+QR   +P G CG+AM P++P K
Sbjct: 314 EDYWIIRNSWGLNWGDSGYVKLQRNIDDPFGKCGIAMMPSYPTK 349

BLAST of Csa1G528520 vs. TAIR10
Match: AT5G45890.1 (AT5G45890.1 senescence-associated gene 12)

HSP 1 Score: 242.3 bits (617), Expect = 4.2e-64
Identity = 135/347 (38.90%), Postives = 199/347 (57.35%), Query Frame = 1

Query: 2   MKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHH-RISRNEHEMDRRFK 61
           +K + +FL  +A  S  C S  L R   ++E  + + +  W + H R+  +  E + R+ 
Sbjct: 3   LKHMQIFL-FVAIFSSFCFSITLSRP-LDNELIMQKRHIEWMTKHGRVYADVKEENNRYV 62

Query: 62  VFKDNAKHVFKVNHM--GKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGG 121
           VFK+N + +  +N +  G++ KL +NQFAD+++DEF   Y +       L ++   ++  
Sbjct: 63  VFKNNVERIEHLNSIPAGRTFKLAVNQFADLTNDEFRSMY-TGFKGVSALSSQSQTKMSP 122

Query: 122 FMYERATN--IPSSIDWRKKGARRM--------CCWAFAAVAAVESIHQIRTNELVSLSE 181
           F Y+  ++  +P S+DWRKKGA           CCWAF+AVAA+E   QI+  +L+SLSE
Sbjct: 123 FRYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGCCWAFSAVAAIEGATQIKKGKLISLSE 182

Query: 182 QEVVDCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDG 241
           Q++VDCD    GC GG   +AFE I   GG+T E+NYPY   D  C  +  N +  +I G
Sbjct: 183 QQLVDCDTNDFGCEGGLMDTAFEHIKATGGLTTESNYPYKGEDATCNSKKTNPKATSITG 242

Query: 242 YENVPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYG 301
           YE+VP N+E ALMKAVAHQP                 G+FT E  C   +DH V  +GYG
Sbjct: 243 YEDVPVNDEQALMKAVAHQPVSVGIEGGGFDFQFYSSGVFTGE--CTTYLDHAVTAIGYG 302

Query: 302 SDEEGD-YWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFP 318
               G  YWII+N +GT+WG +GYM++Q+  ++ QG+CG+AM  ++P
Sbjct: 303 ESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDKQGLCGLAMKASYP 344

BLAST of Csa1G528520 vs. NCBI nr
Match: gi|700210691|gb|KGN65787.1| (hypothetical protein Csa_1G528520 [Cucumis sativus])

HSP 1 Score: 673.3 bits (1736), Expect = 2.1e-190
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK
Sbjct: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120
           VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM
Sbjct: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120

Query: 121 YERATNIPSSIDWRKKGARRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCDYKVGG 180
           YERATNIPSSIDWRKKGARRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCDYKVGG
Sbjct: 121 YERATNIPSSIDWRKKGARRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCDYKVGG 180

Query: 181 CRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENVPRNNEYAL 240
           CRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENVPRNNEYAL
Sbjct: 181 CRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENVPRNNEYAL 240

Query: 241 MKAVAHQPGMFTEENFCGIRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRG 300
           MKAVAHQPGMFTEENFCGIRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRG
Sbjct: 241 MKAVAHQPGMFTEENFCGIRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRG 300

Query: 301 TRNPQGVCGMAMYPAFPVKY 321
           TRNPQGVCGMAMYPAFPVKY
Sbjct: 301 TRNPQGVCGMAMYPAFPVKY 320

BLAST of Csa1G528520 vs. NCBI nr
Match: gi|659108975|ref|XP_008454483.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 552.7 bits (1423), Expect = 4.1e-154
Identity = 276/346 (79.77%), Postives = 291/346 (84.10%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           +MKFLIV LVLIA  SH+CESFELERKDFESEKSLMQLYKRWSSHHRISRN +EM +RFK
Sbjct: 4   VMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRFK 63

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120
           VFKDNAKHVFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  G VGGFM
Sbjct: 64  VFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKT-GHVGGFM 123

Query: 121 YERATNIPSSIDWRKKGARRM--------CCWAFAAVAAVESIHQIRTNELVSLSEQEVV 180
           YE A  IPSSIDWRKKGA            CWAFAAVAAVESIHQI+TNELVSLSEQEVV
Sbjct: 124 YEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 183

Query: 181 DCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENV 240
           DCDY+ GGCRGG Y SAFEF+MENGGITVE+NYPYY GDGYCRRRG  NERV IDGYENV
Sbjct: 184 DCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYENV 243

Query: 241 PRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSDEE 300
           PRNNE+ALMKAVAHQP                 GMFTE++FCG  IDHTVVVVGYGSDEE
Sbjct: 244 PRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGSDEE 303

Query: 301 -GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 321
            GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAM PA+PVKY
Sbjct: 304 DGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

BLAST of Csa1G528520 vs. NCBI nr
Match: gi|659101492|ref|XP_008451632.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 550.8 bits (1418), Expect = 1.6e-153
Identity = 271/345 (78.55%), Postives = 288/345 (83.48%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           +MKF IV L+LIAF+SH+CESFELERKDFESEK+LMQLYKRWSSHHRISRN +EM +RFK
Sbjct: 3   VMKFFIVPLILIAFMSHLCESFELERKDFESEKNLMQLYKRWSSHHRISRNANEMHKRFK 62

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120
           VFKDNAK VFK N MGKSLKLKLNQFADMSDDEF   + SNIT+YKNLHAK  GRVGGFM
Sbjct: 63  VFKDNAKQVFKKNQMGKSLKLKLNQFADMSDDEFRSIHSSNITHYKNLHAKTIGRVGGFM 122

Query: 121 YERATNIPSSIDWRKKGA--------RRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVV 180
           YE A  IPSSIDWRKKGA        R   CWAFAAVAAVESIHQI+TNELVSLSEQEVV
Sbjct: 123 YEHAKEIPSSIDWRKKGAVNAIKDQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 182

Query: 181 DCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENV 240
           DCDYK GGCRGG Y SAFEFIMEN G+T E+NYPY+ GDGYCRRRG   ERVTIDGYENV
Sbjct: 183 DCDYKDGGCRGGHYNSAFEFIMENDGVTAEDNYPYFEGDGYCRRRGGYKERVTIDGYENV 242

Query: 241 PRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSDEE 300
           PRNNE+ALMKAVAHQP                 GMFTEENFCG  IDHTVVVVGYG+DE+
Sbjct: 243 PRNNEHALMKAVAHQPVAVAIASGGFDFKFYGQGMFTEENFCGYNIDHTVVVVGYGTDED 302

Query: 301 GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 321
           GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAM PA+PVKY
Sbjct: 303 GDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 347

BLAST of Csa1G528520 vs. NCBI nr
Match: gi|659108973|ref|XP_008454482.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 546.2 bits (1406), Expect = 3.8e-152
Identity = 273/346 (78.90%), Postives = 293/346 (84.68%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           +MKFLIV LVLIAF  H+CESFELERKDFESEKSLMQLYKRWSSHHRISRN +EM +RFK
Sbjct: 3   VMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRFK 62

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120
           VFKDNAK+VFK NHMG+SLKL+LNQFADMSDDEFS  +GSNITYYKNLHAK  GRVGGFM
Sbjct: 63  VFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK-NGRVGGFM 122

Query: 121 YERATNIPSSIDWRKKGA--------RRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVV 180
           YE A +IPSSIDWRKKGA        R   CWAFAAVAAVESIHQI+TNELVSLSEQEVV
Sbjct: 123 YEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 182

Query: 181 DCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENV 240
           DCDY+  GC GG Y SAFEF+MENGGITVE+NYPYY GDGYCRRRG  NERVTIDGYENV
Sbjct: 183 DCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYENV 242

Query: 241 PRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSDEE 300
           PRNNE+ALMKAVAHQP                 GMFTE++FCG  IDHTVVVVGYG+DEE
Sbjct: 243 PRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGTDEE 302

Query: 301 -GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 321
            GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMA+ PA+PVK+
Sbjct: 303 DGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 347

BLAST of Csa1G528520 vs. NCBI nr
Match: gi|778722411|ref|XP_011658479.1| (PREDICTED: ervatamin-B-like [Cucumis sativus])

HSP 1 Score: 536.2 bits (1380), Expect = 4.0e-149
Identity = 264/349 (75.64%), Postives = 288/349 (82.52%), Query Frame = 1

Query: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60
           +MKFLIV LVL+AF  +ICESFELERKDFESEKSLMQLYKRWSSHHRISRN +EM  RFK
Sbjct: 3   VMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNRFK 62

Query: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAK----VGGRV 120
           VFK+NAKHVFKVN MGKSLKLKLNQFADMSDDEF   Y SNITYYK+LHAK     GGR+
Sbjct: 63  VFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGGRI 122

Query: 121 GGFMYERATNIPSSIDWRKKGA--------RRMCCWAFAAVAAVESIHQIRTNELVSLSE 180
           GGFMYE A NIPSSIDWRKKGA        R   CWAFAAVAAVESIHQI+TNELVSLSE
Sbjct: 123 GGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSE 182

Query: 181 QEVVDCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDG 240
           +EV+DCDY+ GGCRGG Y SAFEF+M+N G+T+E+NYPYY G+GYCRRRG  N+RV IDG
Sbjct: 183 EEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRIDG 242

Query: 241 YENVPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYG 300
           YENVPRNNEYALMKAVAHQP                 GMFTE +FCG  IDHTVVVVGYG
Sbjct: 243 YENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHTVVVVGYG 302

Query: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 321
           +DE+GDYWIIRNQYG +WGMNGYMKMQRG  +PQGVCGMAM PA+PVKY
Sbjct: 303 TDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVKY 351

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CYSEP_RICCO1.7e-8347.84Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1[more]
CYSEP_VIGMU2.4e-8246.53Vignain OS=Vigna mungo PE=1 SV=1[more]
CYSEP_PHAVU1.8e-8045.35Vignain OS=Phaseolus vulgaris PE=2 SV=2[more]
CEP3_ARATH3.9e-8046.09KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana GN=CEP3 PE=2 SV=... [more]
CEP2_ARATH4.3e-7945.53KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LY73_CUCSA1.4e-190100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528520 PE=3 SV=1[more]
A0A0A0KGB1_CUCSA2.8e-14975.64Uncharacterized protein OS=Cucumis sativus GN=Csa_6G490180 PE=3 SV=1[more]
A0A0A0KW34_CUCSA9.0e-10080.09Uncharacterized protein OS=Cucumis sativus GN=Csa_4G013580 PE=3 SV=1[more]
A0A0A0LMU4_CUCSA2.5e-8949.57Uncharacterized protein OS=Cucumis sativus GN=Csa_2G349680 PE=3 SV=1[more]
V4LTA3_EUTSA1.0e-8248.70Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10010486mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48350.12.2e-8146.09 Cysteine proteinases superfamily protein[more]
AT3G48340.12.4e-8045.53 Cysteine proteinases superfamily protein[more]
AT5G50260.18.6e-7844.80 Cysteine proteinases superfamily protein[more]
AT3G19400.11.1e-6443.02 Cysteine proteinases superfamily protein[more]
AT5G45890.14.2e-6438.90 senescence-associated gene 12[more]
Match NameE-valueIdentityDescription
gi|700210691|gb|KGN65787.1|2.1e-190100.00hypothetical protein Csa_1G528520 [Cucumis sativus][more]
gi|659108975|ref|XP_008454483.1|4.1e-15479.77PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|659101492|ref|XP_008451632.1|1.6e-15378.55PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|659108973|ref|XP_008454482.1|3.8e-15278.90PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|778722411|ref|XP_011658479.1|4.0e-14975.64PREDICTED: ervatamin-B-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000668Peptidase_C1A_C
IPR013128Peptidase_C1A
IPR013201Prot_inhib_I29
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0008234cysteine-type peptidase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0008234 cysteine-type peptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa1G528520.1Csa1G528520.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 127..248
score: 7.0
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 127..318
score: 2.2
IPR013128Peptidase C1APANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 1..319
score: 5.6E
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 39..94
score: 1.
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 39..94
score: 1.
NoneNo IPR availableGENE3DG3DSA:3.90.70.10coord: 5..318
score: 1.1
NoneNo IPR availablePANTHERPTHR12411:SF346KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1-RELATEDcoord: 1..319
score: 5.6E
NoneNo IPR availableunknownSSF54001Cysteine proteinasescoord: 35..318
score: 2.27

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Csa1G528520CSPI01G23680Wild cucumber (PI 183967)cpicuB001
Csa1G528520Cucsa.146860Cucumber (Gy14) v1cgycuB210
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa1G528520Wax gourdcuwgoB017
Csa1G528520Wax gourdcuwgoB048
Csa1G528520Cucumber (Chinese Long) v2cucuB020
Csa1G528520Cucumber (Chinese Long) v2cucuB036
Csa1G528520Cucumber (Gy14) v1cgycuB189
Csa1G528520Cucumber (Gy14) v1cgycuB267
Csa1G528520Cucurbita maxima (Rimu)cmacuB219
Csa1G528520Cucurbita maxima (Rimu)cmacuB234
Csa1G528520Cucurbita maxima (Rimu)cmacuB427
Csa1G528520Cucurbita maxima (Rimu)cmacuB812
Csa1G528520Cucurbita moschata (Rifu)cmocuB204
Csa1G528520Cucurbita moschata (Rifu)cmocuB220
Csa1G528520Cucurbita moschata (Rifu)cmocuB416
Csa1G528520Cucurbita moschata (Rifu)cmocuB804
Csa1G528520Melon (DHL92) v3.5.1cumeB028
Csa1G528520Melon (DHL92) v3.5.1cumeB063
Csa1G528520Melon (DHL92) v3.5.1cumeB064
Csa1G528520Watermelon (Charleston Gray)cuwcgB004
Csa1G528520Watermelon (Charleston Gray)cuwcgB047
Csa1G528520Watermelon (97103) v1cuwmB004
Csa1G528520Watermelon (97103) v1cuwmB021
Csa1G528520Watermelon (97103) v1cuwmB073
Csa1G528520Cucurbita pepo (Zucchini)cpecuB507
Csa1G528520Cucurbita pepo (Zucchini)cpecuB529
Csa1G528520Bottle gourd (USVL1VR-Ls)culsiB030
Csa1G528520Bottle gourd (USVL1VR-Ls)culsiB048
Csa1G528520Cucumber (Gy14) v2cgybcuB002
Csa1G528520Cucumber (Gy14) v2cgybcuB099
Csa1G528520Cucumber (Gy14) v2cgybcuB241
Csa1G528520Melon (DHL92) v3.6.1cumedB023
Csa1G528520Melon (DHL92) v3.6.1cumedB057
Csa1G528520Melon (DHL92) v3.6.1cumedB058
Csa1G528520Silver-seed gourdcarcuB0171
Csa1G528520Silver-seed gourdcarcuB0412
Csa1G528520Silver-seed gourdcarcuB0471
Csa1G528520Silver-seed gourdcarcuB1010
Csa1G528520Watermelon (97103) v2cuwmbB015
Csa1G528520Watermelon (97103) v2cuwmbB043