Csa1G043220 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa1G043220
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionKunitz trypsin inhibitor; contains IPR002160 (Proteinase inhibitor I3, Kunitz legume)
LocationChr1 : 4797141 .. 4797758 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAACTTTGCATTACTGTGTTTTCTTTTCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCGGATGCCTCCCCCGATGCGGTCCTCGACACCGACGGAAAGAAGCTTCGAGCCGGTGATCAATATTATATTCTTTCAGTTTACAGCAGAAACAGTGGTGGATTAAGCATCGGCGGTATCTACGGATACGAAAAATGTCCCATCAACATCCTCCCGGAATCATACGATTATTTACACGGTCTACCGGCGACGTTTTCCCCCATAAACCCTAAAAAGGGTGTGGTCCGAGTTTCTACAGATTTGAACATCCAATTCGAGGCGAACACGAGGTGCGGAATATCGACGGTATGGAAAGTAGGTAAGTTTGATGAATACTTGAAGCAGTATTTTGTGACGATGGGGGGAATGAAAGGGAATCCGGGGCGGGAAACAATAGAAAATTGGTTCAAAGTGGAGAAGTATGGTAAGAATTATAAGTTGGTGTATTGTCCAACAGTTTGTAAGTATTGCAAAGTAGTGTGCAAAGATGTGGGATTATTTTACAAGAATGGAAGGAGGGTTATTGCTTTGAATGATGCTCCATTCCCTGTTATGTTCAAGAAAGTTTGA

mRNA sequence

ATGAGAAACTTTGCATTACTGTGTTTTCTTTTCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCGGATGCCTCCCCCGATGCGGTCCTCGACACCGACGGAAAGAAGCTTCGAGCCGGTGATCAATATTATATTCTTTCAGTTTACAGCAGAAACAGTGGTGGATTAAGCATCGGCGGTATCTACGGATACGAAAAATGTCCCATCAACATCCTCCCGGAATCATACGATTATTTACACGGTCTACCGGCGACGTTTTCCCCCATAAACCCTAAAAAGGGTGTGGTCCGAGTTTCTACAGATTTGAACATCCAATTCGAGGCGAACACGAGGTGCGGAATATCGACGGTATGGAAAGTAGGTAAGTTTGATGAATACTTGAAGCAGTATTTTGTGACGATGGGGGGAATGAAAGGGAATCCGGGGCGGGAAACAATAGAAAATTGGTTCAAAGTGGAGAAGTATGGTAAGAATTATAAGTTGGTGTATTGTCCAACAGTTTGTAAGTATTGCAAAGTAGTGTGCAAAGATGTGGGATTATTTTACAAGAATGGAAGGAGGGTTATTGCTTTGAATGATGCTCCATTCCCTGTTATGTTCAAGAAAGTTTGA

Coding sequence (CDS)

ATGAGAAACTTTGCATTACTGTGTTTTCTTTTCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCGGATGCCTCCCCCGATGCGGTCCTCGACACCGACGGAAAGAAGCTTCGAGCCGGTGATCAATATTATATTCTTTCAGTTTACAGCAGAAACAGTGGTGGATTAAGCATCGGCGGTATCTACGGATACGAAAAATGTCCCATCAACATCCTCCCGGAATCATACGATTATTTACACGGTCTACCGGCGACGTTTTCCCCCATAAACCCTAAAAAGGGTGTGGTCCGAGTTTCTACAGATTTGAACATCCAATTCGAGGCGAACACGAGGTGCGGAATATCGACGGTATGGAAAGTAGGTAAGTTTGATGAATACTTGAAGCAGTATTTTGTGACGATGGGGGGAATGAAAGGGAATCCGGGGCGGGAAACAATAGAAAATTGGTTCAAAGTGGAGAAGTATGGTAAGAATTATAAGTTGGTGTATTGTCCAACAGTTTGTAAGTATTGCAAAGTAGTGTGCAAAGATGTGGGATTATTTTACAAGAATGGAAGGAGGGTTATTGCTTTGAATGATGCTCCATTCCCTGTTATGTTCAAGAAAGTTTGA

Protein sequence

MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDVGLFYKNGRRVIALNDAPFPVMFKKV*
Homology
[Close]
BLAST of Csa1G043220 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 179.1 bits (453), Expect = 4.9e-44
Identity = 98/208 (47.12%), Postives = 128/208 (61.54%), Query Frame = 1 

Query: 7   LCFLFI---VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGI 66
           L F F+   + A++      AD++P+ VLD DG+KLR G  YYI+ V   + GGL++   
Sbjct: 9   LSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSAT 68

Query: 67  Y--GYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCG--ISTV 126
              G   CP  ++    +  H  P  F P NPK+ VVRVSTDLNI F A   C    STV
Sbjct: 69  TPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTV 128

Query: 127 WKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKN--YKLVYCPTVCKYCKVVC 186
           W++ K+DE   QYFVT+GG+KGNPG ETI +WFK+E++  +  YKLV+CPTVC  CKV C
Sbjct: 129 WRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKC 188

Query: 187 KDVGLFY-KNGRRVIALNDAPFPVMFKK 205
            DVG++  + GRR +AL+D PF   F K
Sbjct: 189 GDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]
BLAST of Csa1G043220 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 5.7e-32
Identity = 80/209 (38.28%), Postives = 115/209 (55.02%), Query Frame = 1 

Query: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYIL-SVYSRNSGGLS 60
           M+    +  L     S    F  A+A+   VLDTDG +L+ G QYY+L S+     GGL+
Sbjct: 1   MKTATAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLA 60

Query: 61  IGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFE--ANTRCGIS 120
           +G   G + CP  ++    D  +G P  FS  + K  VVRVSTD+NI+F    +  C  S
Sbjct: 61  LGRATG-QSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTS 120

Query: 121 TVWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYG-KNYKLVYCPTVCKYCKVV 180
           TVW++  +D    +++VT  G+KG PG  T+ +WFK+EK G   YK  +CP+VC  C  +
Sbjct: 121 TVWRLDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTL 180

Query: 181 CKDVGLFY-KNGRRVIALNDAPFPVMFKK 205
           C D+G     +G+  +AL+D  +  MFKK
Sbjct: 181 CSDIGRHSDDDGQIRLALSDNEWAWMFKK 208

[Close]
BLAST of Csa1G043220 vs. Swiss-Prot
Match: LSPI_CARPA (Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.4e-21
Identity = 62/183 (33.88%), Postives = 100/183 (54.64%), Query Frame = 1 

Query: 27  SPDAVLDTDGKKLRAGDQYYILS-VYSRNSGGLSIGGIYGYEKCPINILPESYDYLHGLP 86
           +P  ++D DGK +  G  Y+++S ++    GGL++ G    +KCP++++ + +D  +G P
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQDPFD--NGEP 61

Query: 87  ATFSPI-NPKKGVVRVSTDLNIQFEANTRCGISTVWKVGKFDEYLKQYFVTMGGMKGNPG 146
             FS I N K  +V  S DLN++F     C  +T WKV +F   +  + VT+GG KG  G
Sbjct: 62  IIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVI-GWTVTLGGEKGYHG 121

Query: 147 RETIENWFKVEKYGK--NYKLVYCPTVCKYCKVVCKDVGLFYKNG--RRVIALNDAPFPV 204
            E+  + FK++K G   +YK  +CP+  +   + C +V +F+     RR+I  NDA   V
Sbjct: 122 FESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTNDAKEFV 181

[Close]
BLAST of Csa1G043220 vs. Swiss-Prot
Match: DRTI_DELRE (Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.7e-18
Identity = 62/181 (34.25%), Postives = 92/181 (50.83%), Query Frame = 1 

Query: 29  DAVLDTDGKKLRAGDQYYILS-VYSRNSGGLSIGGIYGYEKCPINILPESYDYLHGLPAT 88
           + V D +G  +  G +YYI+S +     GG+  G   G   CP++I+ E  D   GLP  
Sbjct: 4   EKVYDIEGYPVFLGSEYYIVSAIIGAGGGGVRPGRTRG-SMCPMSIIQEQSDLQMGLPVR 63

Query: 89  FSPINPKKGVVRVSTDLNIQFEANTRCGISTVWKVGKFDEYLKQYFVTMGGMKGNPGRET 148
           FS     +G +   T+L I+F     C  S+ W + K     +   V +GG + +P  E 
Sbjct: 64  FSSPEESQGKIYTDTELEIEFVEKPDCAESSKWVIVKDSGEAR---VAIGGSEDHPQGEL 123

Query: 149 IENWFKVEKYGK-NYKLVYCPTVCKYCKVVCKDVGLFYKNGRRVIAL---NDAPFPVMFK 205
           +  +FK+EK G   YKLV+CP   K     C D+G+ Y+ GRR + L   +D+PF V+F 
Sbjct: 124 VRGFFKIEKLGSLAYKLVFCP---KSSSGSCSDIGINYE-GRRSLVLKSSDDSPFRVVFV 176

[Close]
BLAST of Csa1G043220 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 4.6e-18
Identity = 64/183 (34.97%), Postives = 87/183 (47.54%), Query Frame = 1 

Query: 26  ASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGIYGYEKCPINILPESYDYLHGLP 85
           A+P  V DT+G +L A   YY+L     + GGL++        CP+ +  E+ +   G P
Sbjct: 22  AAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAP--RVLPCPLLVAQETDERRKGFP 81

Query: 86  ATFSPIN----PKKGVVRVSTDLNIQFEANTRCGISTVWKVGKFDEYLKQYFVTMGGMKG 145
             F+P      P+   +RVSTD+ I+F A T C  ST W VG  DE L      + G   
Sbjct: 82  VRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVG--DEPLTGARRVVTGPLI 141

Query: 146 NPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDVGLFYKNGRRVIALNDAPFPVM 205
            P     EN F+VEKYG  YKLV        C+  C+D+G+     R  +  +  P  V+
Sbjct: 142 GPSPSGRENAFRVEKYGGGYKLV-------SCRDSCQDLGVSRDGARAWLGASQPPHVVV 193

[Close]
BLAST of Csa1G043220 vs. TrEMBL
Match: A0A0A0LT81_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 1.8e-117
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 1 

Query: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI
Sbjct: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW 120

Query: 121 KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV 180
           KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV
Sbjct: 121 KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV 180

Query: 181 GLFYKNGRRVIALNDAPFPVMFKKV 206
           GLFYKNGRRVIALNDAPFPVMFKKV
Sbjct: 181 GLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]
BLAST of Csa1G043220 vs. TrEMBL
Match: A0A0A0LTW2_CUCSA (Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.0e-72
Identity = 129/207 (62.32%), Postives = 168/207 (81.16%), Query Frame = 1 

Query: 1   MRNFALLC-FLFIVIASSE-VRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGL 60
           M+NF +L  FLFI++AS++ +RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  SIGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGIST 120
           ++G +   EKCP+N++ E  + ++G P TF P+NPKKGVVRVSTDLN+QFEA+T C  ST
Sbjct: 61  TLGNLQS-EKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTST 120

Query: 121 VWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCK 180
           VWK+ KFDE   Q+ VT+GG +GNPG ET++NWFK+EK+GK+YKLV+CPTVC +CKV+C+
Sbjct: 121 VWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCR 180

Query: 181 DVGLFYKNGRRVIALNDAPFPVMFKKV 206
           D+G+F+KNG R +AL+D PFPVMFKKV
Sbjct: 181 DIGIFFKNGERALALSDTPFPVMFKKV 206

[Close]
BLAST of Csa1G043220 vs. TrEMBL
Match: M5X024_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.5e-58
Identity = 109/200 (54.50%), Postives = 144/200 (72.00%), Query Frame = 1 

Query: 7   LCFLFIVIA-SSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGIYG 66
           L F F++ A S+++R   ADA+P  VLD  G KL+ G  YYIL V     GGL++     
Sbjct: 9   LVFCFLLFAFSAKLRSVAADAAPSPVLDITGNKLQTGVDYYILPVIRGRGGGLTLASTSN 68

Query: 67  YEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVWKVGKF 126
              CP++++ E  +  +GLP  FSP+N  KGVVRVSTDLNI+F A T C  STVWK+GKF
Sbjct: 69  KTSCPLDVVQEQNEVSNGLPLKFSPVNVTKGVVRVSTDLNIKFSATTICVQSTVWKLGKF 128

Query: 127 DEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDVGLFYK 186
           DE   Q+FVT GG++GNPGR+T  NWFK+EK+G +YKLV+CPTVC +CKV+C DVG+F++
Sbjct: 129 DEQTGQWFVTSGGVEGNPGRQTTSNWFKIEKFGDDYKLVFCPTVCNFCKVICGDVGIFFQ 188

Query: 187 NGRRVIALNDAPFPVMFKKV 206
           +G+R +AL+D PF  MFKKV
Sbjct: 189 DGKRRLALSDVPFRAMFKKV 208

[Close]
BLAST of Csa1G043220 vs. TrEMBL
Match: A5AXN3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027379 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.6e-57
Identity = 107/204 (52.45%), Postives = 151/204 (74.02%), Query Frame = 1 

Query: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           M+  +LL  L ++  + +     A+++PD VLDT+GKKLR+G  YYIL V+    GGL++
Sbjct: 1   MKTTSLLFSLLLIALAVKPFSVAAESAPDPVLDTEGKKLRSGVDYYILPVFRGRGGGLTL 60

Query: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW 120
               G E CP++++ E  +  +GLP TF+P+NPKKGV+RVSTD NI+F A+T C  ST+W
Sbjct: 61  AST-GNESCPLDVVQEQQEVSNGLPLTFTPVNPKKGVIRVSTDHNIKFSASTICVQSTLW 120

Query: 121 KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV 180
           K+ ++DE   Q+FVT GG++GNPGRET++NWFK+EKY  +YKLV+CPTVC +CK VC D+
Sbjct: 121 KL-EYDESSGQWFVTTGGVEGNPGRETLDNWFKIEKYEDDYKLVFCPTVCDFCKPVCGDI 180

Query: 181 GLFYKNGRRVIALNDAPFPVMFKK 205
           G++ +NG R +AL+D PF VMFKK
Sbjct: 181 GIYIQNGYRRLALSDVPFKVMFKK 202

[Close]
BLAST of Csa1G043220 vs. TrEMBL
Match: V4TSJ0_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023257mg PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 7.9e-57
Identity = 109/206 (52.91%), Postives = 144/206 (69.90%), Query Frame = 1 

Query: 1   MRNFALLCFLFIVIASSEVRF-CRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLS 60
           MRN  +L  L ++ A        R +ASPD VLD  GK+LRAG +YYIL V     GGL+
Sbjct: 1   MRNTLVLPSLILLFAFIATPLPVRGNASPDPVLDIAGKQLRAGSKYYILPVTKGRGGGLT 60

Query: 61  IGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTV 120
           + G    + CP++++ E + + +GLP TFSP+NPKKGVVR STDLNI+F+A T C  STV
Sbjct: 61  LAGRSNNKTCPVDVVQEQHSFRNGLPVTFSPVNPKKGVVRESTDLNIKFDAATSCAQSTV 120

Query: 121 WKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKD 180
           WK+  FD  L Q+ VT GG++GNPG  T+ NWFK+EK+  NYKLVYCP+VC +C+ +C+D
Sbjct: 121 WKLDNFDAALGQWLVTTGGVEGNPGPRTMRNWFKIEKFFGNYKLVYCPSVCNFCRGLCRD 180

Query: 181 VGLFYKNGRRVIALNDAPFPVMFKKV 206
           VG+F   G R +AL+D PF V+FKKV
Sbjct: 181 VGIFINGGVRRLALSDVPFKVVFKKV 206

[Close]
BLAST of Csa1G043220 vs. TAIR10
Match: AT1G17860.1 (AT1G17860.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 171.8 bits (434), Expect = 4.4e-43
Identity = 81/199 (40.70%), Postives = 128/199 (64.32%), Query Frame = 1 

Query: 6   LLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIGGIYG 65
           L  FL + +  S  R    +A+ + V D +GK L  G  YYIL V     GGL++  +  
Sbjct: 5   LYIFLLLAVFISH-RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 64

Query: 66  YEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVWKVGKF 125
            E CP +++ + ++   GLP  FSP + K   + VSTD+NI+F        +++W++  F
Sbjct: 65  -ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSP------TSIWELANF 124

Query: 126 DEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDVGLFYK 185
           DE  KQ+F++  G++GNPG++T++NWFK++K+ K+YK+ +CPTVC +CKV+C+DVG+F +
Sbjct: 125 DETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQ 184

Query: 186 NGRRVIALNDAPFPVMFKK 205
           +G+R +AL+D P  VMFK+
Sbjct: 185 DGKRRLALSDVPLKVMFKR 194

[Close]
BLAST of Csa1G043220 vs. TAIR10
Match: AT1G73325.1 (AT1G73325.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 90.1 bits (222), Expect = 1.7e-18
Identity = 67/211 (31.75%), Postives = 105/211 (49.76%), Query Frame = 1 

Query: 1   MRNFALLCFLFIVIASSEVRFCRADASPD-AVLDTDGKKLRAGDQYYILSVYSRNSGGL- 60
           M    L      V+++       ADA+P   VLD  G  +++  QYYI+       GGL 
Sbjct: 1   MEKLTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLI 60

Query: 61  -SIGGIYGYEKC-PINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANT-RCG 120
            S   +   + C  ++I+  S  ++ GLP TFSP+N K   V++S  LN++F++    C 
Sbjct: 61  PSNRNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCP 120

Query: 121 ISTVWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKV 180
            S VW++    + L++ FV++GG KG        +WF++++ G  YKL+YCP       V
Sbjct: 121 DSKVWRIDHSVQ-LRKSFVSIGGQKGKGN-----SWFQIQEDGDAYKLMYCPI---SSIV 180

Query: 181 VCKDVGLFYKNG--RRVIALNDAPFPVMFKK 205
            C +V L   +   RR++   D  F V F+K
Sbjct: 181 ACINVSLEIDDHGVRRLVLSTDQSFVVKFQK 202

[Close]
BLAST of Csa1G043220 vs. NCBI nr
Match: gi|449439731|ref|XP_004137639.1| (PREDICTED: miraculin [Cucumis sativus])

HSP 1 Score: 429.9 bits (1104), Expect = 2.6e-117
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 1 

Query: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI
Sbjct: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW 120

Query: 121 KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV 180
           KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV
Sbjct: 121 KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV 180

Query: 181 GLFYKNGRRVIALNDAPFPVMFKKV 206
           GLFYKNGRRVIALNDAPFPVMFKKV
Sbjct: 181 GLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]
BLAST of Csa1G043220 vs. NCBI nr
Match: gi|659066987|ref|XP_008437082.1| (PREDICTED: miraculin [Cucumis melo])

HSP 1 Score: 409.1 bits (1050), Expect = 4.7e-111
Identity = 192/205 (93.66%), Postives = 200/205 (97.56%), Query Frame = 1 

Query: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60
           M+NFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESYDYL GLPATFSP+NPKKGVVRVSTDLNI+FEA+TRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVW 120

Query: 121 KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV 180
           KVGKFD+YLKQYFVTMGG KGNPGRETI NWFKVEK+GKNYK VYCPTVCKYCKV+CKDV
Sbjct: 121 KVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDV 180

Query: 181 GLFYKNGRRVIALNDAPFPVMFKKV 206
           GLFYKNGRR+ ALNDAPFPVMFKKV
Sbjct: 181 GLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]
BLAST of Csa1G043220 vs. NCBI nr
Match: gi|659066985|ref|XP_008437071.1| (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 296.6 bits (758), Expect = 3.4e-77
Identity = 146/210 (69.52%), Postives = 170/210 (80.95%), Query Frame = 1 

Query: 1   MRNFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGG- 60
           M+ FALL FLFI  VIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +  + GG 
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LSIGGIYG-YEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGI 120
           L+IG I   Y++C IN++ E Y+   G P TF PINPKKGVVRVSTDLNI+F+A TRC  
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGK-NYKLVYCPTVCKYCKV 180
           STVWK+G FD YL+QYFVT+GG KGNPGRET ENWFK+EKYGK NYKLVYCP VCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 VCKDVGLFYKNGRRVIALNDAPFPVMFKKV 206
           +CKD+G+F  NG R + L+D PFPV+FKKV
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

[Close]
BLAST of Csa1G043220 vs. NCBI nr
Match: gi|659066983|ref|XP_008437058.1| (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 287.3 bits (734), Expect = 2.1e-74
Identity = 126/204 (61.76%), Postives = 168/204 (82.35%), Query Frame = 1 

Query: 2   RNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIG 61
           +NF +  F+FI++AS+E+RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL++G
Sbjct: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62

Query: 62  GIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVWK 121
            +   E CP+N++ E ++ ++G P TF P+NPKKGVVRVSTDLN+QF+A+T C  STVWK
Sbjct: 63  NLQS-EICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 122 VGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDVG 181
           + KFDE   Q+FVT+GG +GNPG ET++NWFK+EK+GK+YKLV+CPTVC +CKV+C+D+G
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 182 LFYKNGRRVIALNDAPFPVMFKKV 206
           +F+KNG+R +AL+D PFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

[Close]
BLAST of Csa1G043220 vs. NCBI nr
Match: gi|449439521|ref|XP_004137534.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 281.2 bits (718), Expect = 1.5e-72
Identity = 129/207 (62.32%), Postives = 168/207 (81.16%), Query Frame = 1 

Query: 1   MRNFALLC-FLFIVIASSE-VRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGL 60
           M+NF +L  FLFI++AS++ +RF  ADASP+AVLD DGKKLRAG  YYIL V+    GGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  SIGGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGIST 120
           ++G +   EKCP+N++ E  + ++G P TF P+NPKKGVVRVSTDLN+QFEA+T C  ST
Sbjct: 61  TLGNLQS-EKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTST 120

Query: 121 VWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCK 180
           VWK+ KFDE   Q+ VT+GG +GNPG ET++NWFK+EK+GK+YKLV+CPTVC +CKV+C+
Sbjct: 121 VWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCR 180

Query: 181 DVGLFYKNGRRVIALNDAPFPVMFKKV 206
           D+G+F+KNG R +AL+D PFPVMFKKV
Sbjct: 181 DIGIFFKNGERALALSDTPFPVMFKKV 206

The following BLAST results are available for this feature:
BLAST of Csa1G043220 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MIRA_SYNDU4.9e-4447.12Miraculin OS=Synsepalum dulcificum PE=1 SV=3[more]
ASP_THECC5.7e-3238.2821 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1[more]
LSPI_CARPA3.4e-2133.88Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1[more]
DRTI_DELRE2.7e-1834.25Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1[more]
IAAS_ORYSJ4.6e-1834.97Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ... [more]
BLAST of Csa1G043220 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LT81_CUCSA1.8e-117100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1[more]
A0A0A0LTW2_CUCSA1.0e-7262.32Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1[more]
M5X024_PRUPE2.5e-5854.50Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1[more]
A5AXN3_VITVI4.6e-5752.45Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027379 PE=4 SV=1[more]
V4TSJ0_9ROSI7.9e-5752.91Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023257mg PE=4 SV=1[more]
BLAST of Csa1G043220 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G17860.14.4e-4340.70 Kunitz family trypsin and protease inhibitor protein[more]
AT1G73325.11.7e-1831.75 Kunitz family trypsin and protease inhibitor protein[more]
BLAST of Csa1G043220 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439731|ref|XP_004137639.1|2.6e-117100.00PREDICTED: miraculin [Cucumis sativus][more]
gi|659066987|ref|XP_008437082.1|4.7e-11193.66PREDICTED: miraculin [Cucumis melo][more]
gi|659066985|ref|XP_008437071.1|3.4e-7769.52PREDICTED: miraculin-like [Cucumis melo][more]
gi|659066983|ref|XP_008437058.1|2.1e-7461.76PREDICTED: miraculin-like [Cucumis melo][more]
gi|449439521|ref|XP_004137534.1|1.5e-7262.32PREDICTED: miraculin-like [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002160Prot_inh_Kunz-lg
IPR011065Kunitz_inhibitor_STI-like_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa1G043220.1Csa1G043220.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumePRINTSPR00291KUNITZINHBTRcoord: 30..59
score: 1.7E-11coord: 151..170
score: 1.7E-11coord: 69..89
score: 1.7
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 31..204
score: 1.1
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 30..205
score: 3.2
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 31..47
scor
IPR011065Kunitz inhibitor ST1-likeunknownSSF50386STI-likecoord: 27..205
score: 1.55
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 27..205
score: 1.1
NoneNo IPR availablePANTHERPTHR33107FAMILY NOT NAMEDcoord: 2..205
score: 5.2
NoneNo IPR availablePANTHERPTHR33107:SF2SUBFAMILY NOT NAMEDcoord: 2..205
score: 5.2

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa1G043220Wax gourdcuwgoB028
Csa1G043220Cucumber (Gy14) v1cgycuB159
Csa1G043220Cucurbita maxima (Rimu)cmacuB217
Csa1G043220Cucurbita maxima (Rimu)cmacuB390
Csa1G043220Cucurbita moschata (Rifu)cmocuB202
Csa1G043220Cucurbita moschata (Rifu)cmocuB380
Csa1G043220Melon (DHL92) v3.5.1cumeB027
Csa1G043220Watermelon (Charleston Gray)cuwcgB021
Csa1G043220Watermelon (97103) v1cuwmB041
Csa1G043220Cucurbita pepo (Zucchini)cpecuB029
Csa1G043220Cucurbita pepo (Zucchini)cpecuB505
Csa1G043220Bottle gourd (USVL1VR-Ls)culsiB022
Csa1G043220Cucumber (Gy14) v2cgybcuB001
Csa1G043220Melon (DHL92) v3.6.1cumedB022
Csa1G043220Silver-seed gourdcarcuB0415
Csa1G043220Silver-seed gourdcarcuB0523
Csa1G043220Watermelon (97103) v2cuwmbB010