Csa1G043210 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa1G043210
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Kunitz trypsin inhibitor; contains IPR002160 (Proteinase inhibitor I3, Kunitz legume)
Location	Chr1 : 4794973 .. 4795611 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATTTTGCATTACTCACTTGTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCGGAGCTGATGCGTCACCAGATCCCGTCCTCGACACCGATGGAAAGAAGCTCAGAATCAGCAACAAATACTACATCCTCCCAGCAATTCAAGGCAGTGTTGGTGGTGGAAGTTTCGCCATCGGGAATATCCGAAAAGAATACGATAGATGTGGTGTCAACGTCGTCTAGGAGTGTTACGAACAACCCGATGGTTTTTCCACTACATTTTTACCAATAAACCTTAAAAAGGGTGTGGTTTGAGTCTCCACCGATTTGAACATCGAGTTTGATGCGACTACAAGGTGTGTGAAATCGACGGTGTGGAGATTAGGAACATTTGATCAATATTTGAGGCAATATTTCGTAACAATTGGCGGAACGAAAGAGAATCCGGGGTGAGAGACTACGGAAAACTGGTTCAAAATAGAGCAATATGGGAAGGTTACTAAGTTGGTGTATTGTCCAAGAGTGGTGTGTAAGAATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACAATGGAATAAAAGGTCTTGTTTTGAGCGATACGCCATTTCCGGTTATGTTCAAGAAAGTTTATGATTGA

mRNA sequence

ATGCATTTTGCATTACTCACTTGTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCGGAGCTGATGCGTCACCAGATCCCGTCCTCGACACCGATGGAAAGAAGCTCAGAATCAGCAACAAATACTACATCCTCCCAGCAATTCAAGGCAGTGTTGGTGGTGGAAGTTTCGCCATCGGGAATATCCGAAAAGAATACGATAGATGTGTCTCCACCGATTTGAACATCGAGTTTGATGCGACTACAAGGTGTGTGAAATCGACGGTGTGGAGATTAGGAACATTTGATCAATATTTGAGGCAATATTTCGTAACAATTGGCGGAACGAAAGAGAATCCGGGAGTGGTGTGTAAGAATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACAATGGAATAAAAGGTCTTGTTTTGAGCGATACGCCATTTCCGGTTATGTTCAAGAAAGTTTATGATTGA

Coding sequence (CDS)

ATGCATTTTGCATTACTCACTTGTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCGGAGCTGATGCGTCACCAGATCCCGTCCTCGACACCGATGGAAAGAAGCTCAGAATCAGCAACAAATACTACATCCTCCCAGCAATTCAAGGCAGTGTTGGTGGTGGAAGTTTCGCCATCGGGAATATCCGAAAAGAATACGATAGATGTGTCTCCACCGATTTGAACATCGAGTTTGATGCGACTACAAGGTGTGTGAAATCGACGGTGTGGAGATTAGGAACATTTGATCAATATTTGAGGCAATATTTCGTAACAATTGGCGGAACGAAAGAGAATCCGGGAGTGGTGTGTAAGAATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACAATGGAATAAAAGGTCTTGTTTTGAGCGATACGCCATTTCCGGTTATGTTCAAGAAAGTTTATGATTGA

Protein sequence

MHFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGSFAIGNIRKEYDRCVSTDLNIEFDATTRCVKSTVWRLGTFDQYLRQYFVTIGGTKENPGVVCKNCKVMCKDIGIFENNGIKGLVLSDTPFPVMFKKVYD*

Homology

[Close]

BLAST of Csa1G043210 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 106.3 bits (264), Expect = 3.1e-22
Identity = 73/201 (36.32%), Postives = 91/201 (45.27%), Query Frame = 1


Query: 12  AIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGG---------GSFA 71
           A++ A++      AD++P+PVLD DG+KLR    YYI+P ++   GG         G+F 
Sbjct: 16  ALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFV 75

Query: 72  ----IGNIRKEYDR----------------CVSTDLNIEFDATTRC--VKSTVWRLGTFD 131
               +   RKE D                  VSTDLNI F A   C    STVWRL  +D
Sbjct: 76  CPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYD 135

Query: 132 QYLRQYFVTIGGTKENPG-------------------------VVCKNCKVMCKDIGIF- 156
           +   QYFVTIGG K NPG                          VC +CKV C D+GI+ 
Sbjct: 136 ESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYI 195

[Close]

BLAST of Csa1G043210 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 2.7e-13
Identity = 64/205 (31.22%), Postives = 88/205 (42.93%), Query Frame = 1


Query: 7   TCLFIAIVIASSEVRFCG-ADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGSFAIGN 66
           T + + +   +S+  F G A+A+  PVLDTDG +L+   +YY+L +I G+ GGG  A+G 
Sbjct: 5   TAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGA-GGGGLALGR 64

Query: 67  I------------RKEYDR----------------CVSTDLNIEF--DATTRCVKSTVWR 126
                        R + D                  VSTD+NIEF       C  STVWR
Sbjct: 65  ATGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWR 124

Query: 127 LGTFDQYLRQYFVTIGGTKENPG------------------------VVCKNCKVMCKDI 156
           L  +D    +++VT  G K  PG                         VC +C  +C DI
Sbjct: 125 LDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDI 184

[Close]

BLAST of Csa1G043210 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 4.4e-08
Identity = 53/193 (27.46%), Postives = 74/193 (38.34%), Query Frame = 1


Query: 6   LTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGSFAI-- 65
           +  L + +++ S         A+P PV DT+G +L     YY+LPA  G  GG + A   
Sbjct: 1   MVSLRLPLILLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV 60

Query: 66  -------------------------GNIRKEYDRC--VSTDLNIEFDATTRCVKSTVWRL 125
                                    G      DR   VSTD+ I F+A T CV+ST W +
Sbjct: 61  LPCPLLVAQETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHV 120

Query: 126 GTFDQYLRQYFVT---IGGT-----------KENPGVVCKNCKVMCKDIGIFENNGIKGL 156
           G       +  VT   IG +           K   G    +C+  C+D+G+  +     L
Sbjct: 121 GDEPLTGARRVVTGPLIGPSPSGRENAFRVEKYGGGYKLVSCRDSCQDLGVSRDGARAWL 180

[Close]

BLAST of Csa1G043210 vs. TrEMBL
Match: A0A0A0LR02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043210 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 9.6e-87
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 1


Query: 1   MHFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGS 60
           MHFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGS
Sbjct: 1   MHFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGS 60

Query: 61  FAIGNIRKEYDRCVSTDLNIEFDATTRCVKSTVWRLGTFDQYLRQYFVTIGGTKENPGVV 120
           FAIGNIRKEYDRCVSTDLNIEFDATTRCVKSTVWRLGTFDQYLRQYFVTIGGTKENPGVV
Sbjct: 61  FAIGNIRKEYDRCVSTDLNIEFDATTRCVKSTVWRLGTFDQYLRQYFVTIGGTKENPGVV 120

Query: 121 CKNCKVMCKDIGIFENNGIKGLVLSDTPFPVMFKKVYD 159
           CKNCKVMCKDIGIFENNGIKGLVLSDTPFPVMFKKVYD
Sbjct: 121 CKNCKVMCKDIGIFENNGIKGLVLSDTPFPVMFKKVYD 158

[Close]

BLAST of Csa1G043210 vs. TrEMBL
Match: A0A0A0LT81_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.3e-38
Identity = 99/205 (48.29%), Postives = 118/205 (57.56%), Query Frame = 1


Query: 2   HFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGSF 61
           +FALL  LFI  VIASSEVRFC ADASPD VLDTDGKKLR  ++YYIL     + GG S 
Sbjct: 3   NFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 62

Query: 62  A---------IGNIRKEYDRC------------------VSTDLNIEFDATTRCVKSTVW 121
                     I  + + YD                    VSTDLNI+F+A TRC  STVW
Sbjct: 63  GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW 122

Query: 122 RLGTFDQYLRQYFVTIGGTKENPG-----------------------VVCKNCKVMCKDI 157
           ++G FD+YL+QYFVT+GG K NPG                        VCK CKV+CKD+
Sbjct: 123 KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV 182

[Close]

BLAST of Csa1G043210 vs. TrEMBL
Match: A0A0A0LTW2_CUCSA (Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.0e-32
Identity = 88/201 (43.78%), Postives = 111/201 (55.22%), Query Frame = 1


Query: 10  FIAIVIASSE-VRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGSFAIGNIRK 69
           F+ I++AS++ +RF  ADASP+ VLD DGKKLR    YYILP  +G   GG   +GN++ 
Sbjct: 10  FLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGR--GGGLTLGNLQS 69

Query: 70  EYDRC------------------------------VSTDLNIEFDATTRCVKSTVWRLGT 129
           E  +C                              VSTDLN++F+A+T CV STVW+L  
Sbjct: 70  E--KCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDK 129

Query: 130 FDQYLRQYFVTIGGTKENPGV-----------------------VCKNCKVMCKDIGIFE 157
           FD+   Q+ VTIGG++ NPGV                       VC  CKVMC+DIGIF 
Sbjct: 130 FDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFF 189

[Close]

BLAST of Csa1G043210 vs. TrEMBL
Match: M5X024_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.6e-25
Identity = 78/191 (40.84%), Postives = 97/191 (50.79%), Query Frame = 1


Query: 17  SSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGS---------------- 76
           S+++R   ADA+P PVLD  G KL+    YYILP I+G  GG +                
Sbjct: 19  SAKLRSVAADAAPSPVLDITGNKLQTGVDYYILPVIRGRGGGLTLASTSNKTSCPLDVVQ 78

Query: 77  ------------FAIGNIRKEYDRCVSTDLNIEFDATTRCVKSTVWRLGTFDQYLRQYFV 136
                       F+  N+ K   R VSTDLNI+F ATT CV+STVW+LG FD+   Q+FV
Sbjct: 79  EQNEVSNGLPLKFSPVNVTKGVVR-VSTDLNIKFSATTICVQSTVWKLGKFDEQTGQWFV 138

Query: 137 TIGGTKENPG-----------------------VVCKNCKVMCKDIGIFENNGIKGLVLS 157
           T GG + NPG                        VC  CKV+C D+GIF  +G + L LS
Sbjct: 139 TSGGVEGNPGRQTTSNWFKIEKFGDDYKLVFCPTVCNFCKVICGDVGIFFQDGKRRLALS 198

[Close]

BLAST of Csa1G043210 vs. TrEMBL
Match: A0A061EZK2_THECC (Kunitz family trypsin and protease inhibitor protein OS=Theobroma cacao GN=TCM_025233 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.8e-25
Identity = 73/181 (40.33%), Postives = 93/181 (51.38%), Query Frame = 1


Query: 25  ADASPDPVLDTDGKKLRISNKYYILPAIQGSVGG-------------------------- 84
           A+A+PDPVLD  GKKLR    YYILP  +G  GG                          
Sbjct: 15  ANAAPDPVLDISGKKLRTGTDYYILPVFRGRGGGLTLASTGNESCPLDVVQEQLEVSDGL 74

Query: 85  -GSFAIGNIRKEYDRCVSTDLNIEFDATTRCVKSTVWRLGTFDQYLRQYFVTIGGTKENP 144
             +F+  NI+K   R VSTD NI+F A T CV+ T+W+L +FD   RQ+FVT GG + NP
Sbjct: 75  PVTFSPVNIKKGVVR-VSTDQNIKFSAATICVQPTLWKLDSFDDSTRQWFVTTGGVEGNP 134

Query: 145 G-----------------------VVCKNCKVMCKDIGIFENNGIKGLVLSDTPFPVMFK 156
           G                        VC  CKVMC+D+G+F + G++ L LSD PF VMFK
Sbjct: 135 GRETIDNWFKIEKYEDDYKLVFCPTVCDFCKVMCRDVGVFIDGGVRRLALSDVPFKVMFK 194

[Close]

BLAST of Csa1G043210 vs. TAIR10
Match: AT1G17860.1 (AT1G17860.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 93.2 bits (230), Expect = 1.5e-19
Identity = 64/207 (30.92%), Postives = 91/207 (43.96%), Query Frame = 1


Query: 1   MHFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGS 60
           M   L   L +A+ I+   V     +A+ +PV D +GK L     YYILP I+G   GG 
Sbjct: 1   MSSLLYIFLLLAVFISHRGVT---TEAAVEPVKDINGKSLLTGVNYYILPVIRGR--GGG 60

Query: 61  FAIGNIRKE-----------------------YDRC----VSTDLNIEFDATTRCVKSTV 120
             + N++ E                       YD+     VSTD+NI+F  T+      +
Sbjct: 61  LTMSNLKTETCPTSVIQDQFEVSQGLPVKFSPYDKSRTIPVSTDVNIKFSPTS------I 120

Query: 121 WRLGTFDQYLRQYFVTIGGTKENPG-----------------------VVCKNCKVMCKD 158
           W L  FD+  +Q+F++  G + NPG                        VC  CKV+C+D
Sbjct: 121 WELANFDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRD 180

[Close]

BLAST of Csa1G043210 vs. TAIR10
Match: AT1G73260.1 (AT1G73260.1 kunitz trypsin inhibitor 1)

HSP 1 Score: 68.2 bits (165), Expect = 5.3e-12
Identity = 56/176 (31.82%), Postives = 71/176 (40.34%), Query Frame = 1


Query: 32  VLDTDGKKLRISNKYYILPAIQGSVGGGSFA----------IGNIRKEYDRCV------- 91
           V+D DG  +     YY+LP I+G  GG + A          I     E D  +       
Sbjct: 30  VVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDEGIPVKFSNW 89

Query: 92  ---------STDLNIEFDA-TTRCVKSTVWRLGTFDQYLRQYFVTIGGTKENPGV----- 151
                    S +LNIE D   T C++ST WR+G FD   +QYFV  G   E  G      
Sbjct: 90  RLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLKS 149

Query: 152 -------------------VCKNCKVMCKDIGIF-ENNGIKGLVLSDTPFPVMFKK 156
                               C +    C D+GIF +  G++ L LSD PF VMFKK
Sbjct: 150 FFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKK 204

[Close]

BLAST of Csa1G043210 vs. NCBI nr
Match: gi|700209122|gb|KGN64218.1| (hypothetical protein Csa_1G043210 [Cucumis sativus])

HSP 1 Score: 327.4 bits (838), Expect = 1.4e-86
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 1


Query: 1   MHFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGS 60
           MHFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGS
Sbjct: 1   MHFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGS 60

Query: 61  FAIGNIRKEYDRCVSTDLNIEFDATTRCVKSTVWRLGTFDQYLRQYFVTIGGTKENPGVV 120
           FAIGNIRKEYDRCVSTDLNIEFDATTRCVKSTVWRLGTFDQYLRQYFVTIGGTKENPGVV
Sbjct: 61  FAIGNIRKEYDRCVSTDLNIEFDATTRCVKSTVWRLGTFDQYLRQYFVTIGGTKENPGVV 120

Query: 121 CKNCKVMCKDIGIFENNGIKGLVLSDTPFPVMFKKVYD 159
           CKNCKVMCKDIGIFENNGIKGLVLSDTPFPVMFKKVYD
Sbjct: 121 CKNCKVMCKDIGIFENNGIKGLVLSDTPFPVMFKKVYD 158

[Close]

BLAST of Csa1G043210 vs. NCBI nr
Match: gi|659066985|ref|XP_008437071.1| (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 248.8 bits (634), Expect = 6.2e-63
Identity = 136/210 (64.76%), Postives = 145/210 (69.05%), Query Frame = 1


Query: 3   FALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGSFA 62
           FALL+ LFIAIVIASSE+RFC ADASPD VLDTDGKKLR+SNKYYILPA +GS GGG  A
Sbjct: 4   FALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGS-GGGGLA 63

Query: 63  IGNIRKEYDRC------------------------------VSTDLNIEFDATTRCVKST 122
           IGNIRKEYDRC                              VSTDLNIEFDATTRC KST
Sbjct: 64  IGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKST 123

Query: 123 VWRLGTFDQYLRQYFVTIGGTKENPG------------------------VVCKNCKVMC 159
           VW+LGTFD+YLRQYFVTIGGTK NPG                         VCK CKVMC
Sbjct: 124 VWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMC 183

[Close]

BLAST of Csa1G043210 vs. NCBI nr
Match: gi|659066987|ref|XP_008437082.1| (PREDICTED: miraculin [Cucumis melo])

HSP 1 Score: 172.9 bits (437), Expect = 4.4e-40
Identity = 103/205 (50.24%), Postives = 119/205 (58.05%), Query Frame = 1


Query: 2   HFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGSF 61
           +FALL  LFI  VIASSEVRFC ADASPD VLDTDGKKLR  ++YYIL     + GG S 
Sbjct: 3   NFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 62

Query: 62  A---------IGNIRKEYDRC------------------VSTDLNIEFDATTRCVKSTVW 121
                     I  + + YD                    VSTDLNIEF+A+TRC  STVW
Sbjct: 63  GGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVW 122

Query: 122 RLGTFDQYLRQYFVTIGGTKENPG-----------------------VVCKNCKVMCKDI 157
           ++G FDQYL+QYFVT+GGTK NPG                        VCK CKVMCKD+
Sbjct: 123 KVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDV 182

[Close]

BLAST of Csa1G043210 vs. NCBI nr
Match: gi|449439731|ref|XP_004137639.1| (PREDICTED: miraculin [Cucumis sativus])

HSP 1 Score: 167.5 bits (423), Expect = 1.8e-38
Identity = 99/205 (48.29%), Postives = 118/205 (57.56%), Query Frame = 1


Query: 2   HFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGSF 61
           +FALL  LFI  VIASSEVRFC ADASPD VLDTDGKKLR  ++YYIL     + GG S 
Sbjct: 3   NFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 62

Query: 62  A---------IGNIRKEYDRC------------------VSTDLNIEFDATTRCVKSTVW 121
                     I  + + YD                    VSTDLNI+F+A TRC  STVW
Sbjct: 63  GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW 122

Query: 122 RLGTFDQYLRQYFVTIGGTKENPG-----------------------VVCKNCKVMCKDI 157
           ++G FD+YL+QYFVT+GG K NPG                        VCK CKV+CKD+
Sbjct: 123 KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV 182

[Close]

BLAST of Csa1G043210 vs. NCBI nr
Match: gi|659066983|ref|XP_008437058.1| (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 156.4 bits (394), Expect = 4.2e-35
Identity = 91/198 (45.96%), Postives = 110/198 (55.56%), Query Frame = 1


Query: 10  FIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGSFAIGNIRKE 69
           FI I++AS+E+RF  ADASP+ VLD DGKKLR    YYILP  +G   GG   +GN++ E
Sbjct: 10  FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGR--GGGLTLGNLQSE 69

Query: 70  YDRC----------------------------VSTDLNIEFDATTRCVKSTVWRLGTFDQ 129
                                           VSTDLN++FDA+T CV STVW+L  FD+
Sbjct: 70  ICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKLDKFDE 129

Query: 130 YLRQYFVTIGGTKENPGV-----------------------VCKNCKVMCKDIGIFENNG 157
              Q+FVTIGG++ NPGV                       VC  CKVMC+DIGIF  NG
Sbjct: 130 STGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNG 189

The following BLAST results are available for this feature:

BLAST of Csa1G043210 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MIRA_SYNDU	3.1e-22	36.32	Miraculin OS=Synsepalum dulcificum PE=1 SV=3	[more]
ASP_THECC	2.7e-13	31.22	21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1	[more]
IAAS_ORYSJ	4.4e-08	27.46	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ...	[more]

BLAST of Csa1G043210 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LR02_CUCSA	9.6e-87	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043210 PE=4 SV=1	[more]
A0A0A0LT81_CUCSA	1.3e-38	48.29	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1	[more]
A0A0A0LTW2_CUCSA	1.0e-32	43.78	Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1	[more]
M5X024_PRUPE	1.6e-25	40.84	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011496mg PE=4 SV=1	[more]
A0A061EZK2_THECC	2.8e-25	40.33	Kunitz family trypsin and protease inhibitor protein OS=Theobroma cacao GN=TCM_0...	[more]

BLAST of Csa1G043210 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G17860.1	1.5e-19	30.92	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	5.3e-12	31.82	kunitz trypsin inhibitor 1	[more]

BLAST of Csa1G043210 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700209122\|gb\|KGN64218.1\|	1.4e-86	100.00	hypothetical protein Csa_1G043210 [Cucumis sativus]	[more]
gi\|659066985\|ref\|XP_008437071.1\|	6.2e-63	64.76	PREDICTED: miraculin-like [Cucumis melo]	[more]
gi\|659066987\|ref\|XP_008437082.1\|	4.4e-40	50.24	PREDICTED: miraculin [Cucumis melo]	[more]
gi\|449439731\|ref\|XP_004137639.1\|	1.8e-38	48.29	PREDICTED: miraculin [Cucumis sativus]	[more]
gi\|659066983\|ref\|XP_008437058.1\|	4.2e-35	45.96	PREDICTED: miraculin-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002160	Prot_inh_Kunz-lg
IPR011065	Kunitz_inhibitor_STI-like_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa1G043210.1	Csa1G043210.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 119..155 score: 1.8E-6coord: 74..118 score: 1.7E-8coord: 32..63 score: 1.
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 31..157 score: 3.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 28..157 score: 5.79
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 29..65 score: 4.4E-9coord: 74..118 score: 9.0E-11coord: 119..156 score: 2.
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 1..158 score: 3.9
None	No IPR available	PANTHER	PTHR33107:SF2	SUBFAMILY NOT NAMED	coord: 1..158 score: 3.9

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa1G043210	Bottle gourd (USVL1VR-Ls)	culsiB022
Csa1G043210	Cucumber (Gy14) v2	cgybcuB001
Csa1G043210	Melon (DHL92) v3.6.1	cumedB022
Csa1G043210	Silver-seed gourd	carcuB0415
Csa1G043210	Silver-seed gourd	carcuB0523
Csa1G043210	Watermelon (97103) v2	cuwmbB010
Csa1G043210	Wax gourd	cuwgoB028
Csa1G043210	Cucumber (Gy14) v1	cgycuB159
Csa1G043210	Cucurbita maxima (Rimu)	cmacuB217
Csa1G043210	Cucurbita maxima (Rimu)	cmacuB390
Csa1G043210	Cucurbita moschata (Rifu)	cmocuB202
Csa1G043210	Cucurbita moschata (Rifu)	cmocuB380
Csa1G043210	Melon (DHL92) v3.5.1	cumeB027
Csa1G043210	Watermelon (Charleston Gray)	cuwcgB021
Csa1G043210	Watermelon (97103) v1	cuwmB041
Csa1G043210	Cucurbita pepo (Zucchini)	cpecuB029
Csa1G043210	Cucurbita pepo (Zucchini)	cpecuB505