CsGy6G025400 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy6G025400
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	aspartic proteinase nepenthesin-2-like
Location	Chr6 : 24944076 .. 24945461 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTTTTACCCATTCCATTTCTCTTTTCCATTTTTCTCCTTCTTCCCACTTCATCTTCTTCCTCCACCACAGTACTCCCACTCACTACTTTTCCTTCAGTTTCATTTACAGATCCATTCAAAACCATCAACCTTCTTCTCTCTGCTTCACTCAACAGAGCTCAACATCTCAAAACCCCACAATCAAAGTCCAACACTTCCATACAGAATGTCTCTCTCTTCCCTCGTAGCTACGGAGCTTACTCGGTTTCCCTCGCCTTCGGAACTCCACCGCAGAACTTATCGTTTATCTTCGATACTGGAAGTAGTCTCGTCTGGTTCCCCTGCACCGCTGGTTATCGTTGTTCCCGTTGTTCGTTTCCCTATGTGGATCCTGCAACGATTTCGAAATTTGTTCCTAAGTTATCTTCCTCTGTGAAGGTTGTTGGTTGTCGAAATCCTAAATGTGCGTGGATTTTTGGCCCTAATTTGAAGTCCAGATGTAGAAATTGTAACTCTAAATCTCGAAAATGTTCCGATTCTTGTCCTGGTTATGGACTTCAGTACGGCTCTGGCGCAACCGCTGGAATTCTCCTCTCTGAAACGCTCGATTTAGAGAATAAACGAGTGCCGGATTTTCTCGTTGGTTGTTCCGTTATGTCTGTTCATCAACCAGCCGGCATTGCCGGATTTGGCCGCGGTCCTGAATCGTTGCCGTCCCAAATGCGACTCAAACGATTCTCCCATTGCCTCGTTTCTCGCGGGTTCGACGACTCGCCAGTGAGTAGTCCTCTAGTACTTGACTCCGGTTCGGAATCCGATGAATCGAAAACTAAGAGTTTCATTTACGCACCCTTCCGAGAGAATCCATCAGTATCCAACGCCGCATTTCGAGAGTACTATTACCTTAGTCTTCGGAGAATCCTCATCGGTGGAAAGCCGGTGAAATTCCCGTACAAGTATCTCGTGCCGGATTCCACCGGAAACGGAGGCGCGATAATCGATTCCGGTTCAACGTTTACGTTTCTAGATAAGCCGATTTTCGAAGCCATAGCGGATGAATTGGAGAAGCAGCTGGTGAAATATCCTCGAGCTAAGGACGTTGAAGCGCAGTCGGGTTTGAGGCCATGCTTTAATATTCCCAAGGAGGAGGAATCAGCGGAGTTTCCGGACGTGGTTTTGAAGTTTAAAGGTGGAGGGAAGCTGAGTTTGGCGGCAGAGAATTACTTGGCGATGGTGACGGATGAGGGCGTGGTGTGCTTGACGATGATGACGGATGAAGCCGTCGTGGGCGGAGGCGGAGGGCCGGCGATTATATTGGGGGCGTTTCAGCAGCAGAATGTTTTGGTTGAGTATGATTTAGCAAAGCAGCGAATCGGATTTCGGAAGCAGAAATGCACGTGA

mRNA sequence

ATGGAGTTTTTACCCATTCCATTTCTCTTTTCCATTTTTCTCCTTCTTCCCACTTCATCTTCTTCCTCCACCACAGTACTCCCACTCACTACTTTTCCTTCAGTTTCATTTACAGATCCATTCAAAACCATCAACCTTCTTCTCTCTGCTTCACTCAACAGAGCTCAACATCTCAAAACCCCACAATCAAAGTCCAACACTTCCATACAGAATGTCTCTCTCTTCCCTCGTAGCTACGGAGCTTACTCGGTTTCCCTCGCCTTCGGAACTCCACCGCAGAACTTATCGTTTATCTTCGATACTGGAAGTAGTCTCGTCTGGTTCCCCTGCACCGCTGGTTATCGTTGTTCCCGTTGTTCGTTTCCCTATGTGGATCCTGCAACGATTTCGAAATTTGTTCCTAAGTTATCTTCCTCTGTGAAGGTTGTTGGTTGTCGAAATCCTAAATGTGCGTGGATTTTTGGCCCTAATTTGAAGTCCAGATGTAGAAATTGTAACTCTAAATCTCGAAAATGTTCCGATTCTTGTCCTGGTTATGGACTTCAGTACGGCTCTGGCGCAACCGCTGGAATTCTCCTCTCTGAAACGCTCGATTTAGAGAATAAACGAGTGCCGGATTTTCTCGTTGGTTGTTCCGTTATGTCTGTTCATCAACCAGCCGGCATTGCCGGATTTGGCCGCGGTCCTGAATCGTTGCCGTCCCAAATGCGACTCAAACGATTCTCCCATTGCCTCGTTTCTCGCGGGTTCGACGACTCGCCAGTGAGTAGTCCTCTAGTACTTGACTCCGGTTCGGAATCCGATGAATCGAAAACTAAGAGTTTCATTTACGCACCCTTCCGAGAGAATCCATCAGTATCCAACGCCGCATTTCGAGAGTACTATTACCTTAGTCTTCGGAGAATCCTCATCGGTGGAAAGCCGGTGAAATTCCCGTACAAGTATCTCGTGCCGGATTCCACCGGAAACGGAGGCGCGATAATCGATTCCGGTTCAACGTTTACGTTTCTAGATAAGCCGATTTTCGAAGCCATAGCGGATGAATTGGAGAAGCAGCTGGTGAAATATCCTCGAGCTAAGGACGTTGAAGCGCAGTCGGGTTTGAGGCCATGCTTTAATATTCCCAAGGAGGAGGAATCAGCGGAGTTTCCGGACGTGGTTTTGAAGTTTAAAGGTGGAGGGAAGCTGAGTTTGGCGGCAGAGAATTACTTGGCGATGGTGACGGATGAGGGCGTGGTGTGCTTGACGATGATGACGGATGAAGCCGTCGTGGGCGGAGGCGGAGGGCCGGCGATTATATTGGGGGCGTTTCAGCAGCAGAATGTTTTGGTTGAGTATGATTTAGCAAAGCAGCGAATCGGATTTCGGAAGCAGAAATGCACGTGA

Coding sequence (CDS)

ATGGAGTTTTTACCCATTCCATTTCTCTTTTCCATTTTTCTCCTTCTTCCCACTTCATCTTCTTCCTCCACCACAGTACTCCCACTCACTACTTTTCCTTCAGTTTCATTTACAGATCCATTCAAAACCATCAACCTTCTTCTCTCTGCTTCACTCAACAGAGCTCAACATCTCAAAACCCCACAATCAAAGTCCAACACTTCCATACAGAATGTCTCTCTCTTCCCTCGTAGCTACGGAGCTTACTCGGTTTCCCTCGCCTTCGGAACTCCACCGCAGAACTTATCGTTTATCTTCGATACTGGAAGTAGTCTCGTCTGGTTCCCCTGCACCGCTGGTTATCGTTGTTCCCGTTGTTCGTTTCCCTATGTGGATCCTGCAACGATTTCGAAATTTGTTCCTAAGTTATCTTCCTCTGTGAAGGTTGTTGGTTGTCGAAATCCTAAATGTGCGTGGATTTTTGGCCCTAATTTGAAGTCCAGATGTAGAAATTGTAACTCTAAATCTCGAAAATGTTCCGATTCTTGTCCTGGTTATGGACTTCAGTACGGCTCTGGCGCAACCGCTGGAATTCTCCTCTCTGAAACGCTCGATTTAGAGAATAAACGAGTGCCGGATTTTCTCGTTGGTTGTTCCGTTATGTCTGTTCATCAACCAGCCGGCATTGCCGGATTTGGCCGCGGTCCTGAATCGTTGCCGTCCCAAATGCGACTCAAACGATTCTCCCATTGCCTCGTTTCTCGCGGGTTCGACGACTCGCCAGTGAGTAGTCCTCTAGTACTTGACTCCGGTTCGGAATCCGATGAATCGAAAACTAAGAGTTTCATTTACGCACCCTTCCGAGAGAATCCATCAGTATCCAACGCCGCATTTCGAGAGTACTATTACCTTAGTCTTCGGAGAATCCTCATCGGTGGAAAGCCGGTGAAATTCCCGTACAAGTATCTCGTGCCGGATTCCACCGGAAACGGAGGCGCGATAATCGATTCCGGTTCAACGTTTACGTTTCTAGATAAGCCGATTTTCGAAGCCATAGCGGATGAATTGGAGAAGCAGCTGGTGAAATATCCTCGAGCTAAGGACGTTGAAGCGCAGTCGGGTTTGAGGCCATGCTTTAATATTCCCAAGGAGGAGGAATCAGCGGAGTTTCCGGACGTGGTTTTGAAGTTTAAAGGTGGAGGGAAGCTGAGTTTGGCGGCAGAGAATTACTTGGCGATGGTGACGGATGAGGGCGTGGTGTGCTTGACGATGATGACGGATGAAGCCGTCGTGGGCGGAGGCGGAGGGCCGGCGATTATATTGGGGGCGTTTCAGCAGCAGAATGTTTTGGTTGAGTATGATTTAGCAAAGCAGCGAATCGGATTTCGGAAGCAGAAATGCACGTGA

Protein sequence

MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTDEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT

Homology

[Close]

BLAST of CsGy6G025400 vs. NCBI nr
Match: XP_011657732.1 (PREDICTED: aspartic proteinase nepenthesin-2-like [Cucumis sativus] >KGN48299.1 hypothetical protein Csa_6G454470 [Cucumis sativus])

HSP 1 Score: 882.9 bits (2280), Expect = 4.8e-253
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS
Sbjct: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG
Sbjct: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR
Sbjct: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXX 420
           DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXX
Sbjct: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXX 420

Query: 421 XXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           XXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 XXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 461

[Close]

BLAST of CsGy6G025400 vs. NCBI nr
Match: XP_008462617.1 (PREDICTED: aspartic proteinase nepenthesin-2-like [Cucumis melo])

HSP 1 Score: 813.1 bits (2099), Expect = 4.7e-232
Identity = 405/461 (87.85%), Postives = 421/461 (91.32%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSS T LPL TFPS+ FTDP KTIN LLSASL+RAQHLK+
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSIT-LPLATFPSIPFTDPLKTINHLLSASLSRAQHLKS 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTS +NVSLFPRSYGAY+VSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRC+ CS
Sbjct: 61  PQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAHCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FP+VDPATISKFVPKLSSSVK+VGCRNPKCAWIFGPNLKSRCRNCN KSRKCSDSCPGYG
Sbjct: 121 FPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           +QYGSGATAGILLSETLDL+NKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 IQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCL+ RGFDDSPVSSPLVLDSG ESDESKTKSFIYAPF+ENPS SN AFREYYYLSLR
Sbjct: 241 FSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTG GGAIIDSGSTFTFLDKPIFEAIA ELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXX 420
           D+EA++GLRPCFNI KEEESAEFP+V LKFKGGGKLSL  ENYL MVTD  VVCLT    
Sbjct: 361 DIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMMTN 420

Query: 421 XXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
                         GAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 AEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of CsGy6G025400 vs. NCBI nr
Match: XP_023543736.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 700.3 bits (1806), Expect = 4.4e-198
Identity = 351/463 (75.81%), Postives = 382/463 (82.51%), Query Frame = 0


Query: 1   MEFLPIPFLFS--IFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHL 60
           MEF PIPFL S                  PLT FPS+ FT P+K I  L+SASL RAQHL
Sbjct: 1   MEFFPIPFLLSXXXXXXXXXXXXXXXXXXPLTVFPSLPFTHPWKNIKHLVSASLTRAQHL 60

Query: 61  KTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSR 120
           KTP+ KSNTSIQNV+LFPRSYGAYS+SLAFGTPPQ+LS +FDTGSSLVWFPCTAGYRCS 
Sbjct: 61  KTPRIKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPG 180
           CSFP VD ATI KF+PKLSSS K++GCRN KC+WIFGPNLKS CR+C+ +SRKCSD+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKSLCRSCSPRSRKCSDTCPG 180

Query: 181 YGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL 240
           YG+QYGSGATAG LLSETLD   KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLS 300
           KRFSHCLV R FDDSPVSSPLVLDS SES ESK  S IYAPFRENPS SNAAFREYYYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+S GNGGAIIDSGSTFTFLDKPIFEA+A+ELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 AKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXX 420
           AK VEA+SGLRPCF+I K EES EFP+++LKFKGG  L+L   NYLA+VTD GVVCLT  
Sbjct: 361 AKGVEAESGLRPCFDISK-EESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMI 420

Query: 421 XXXXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
                           GAFQQQNVLV+YDLAK RIGFRKQ+CT
Sbjct: 421 TDVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKDRIGFRKQRCT 462

[Close]

BLAST of CsGy6G025400 vs. NCBI nr
Match: XP_022925946.1 (probable aspartyl protease At4g16563 [Cucurbita moschata])

HSP 1 Score: 693.3 bits (1788), Expect = 5.4e-196
Identity = 347/463 (74.95%), Postives = 381/463 (82.29%), Query Frame = 0


Query: 1   MEFLPIPFLFS--IFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHL 60
           MEF  IPFL S                 LPLT FPS+ F  P+K I  L+SASL RAQHL
Sbjct: 1   MEFFLIPFLLSXXXXXXXXXXXXXXXXXLPLTVFPSLPFAHPWKNIKHLVSASLTRAQHL 60

Query: 61  KTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSR 120
           KTP++KSNTSIQNV+LFPRSYGAYS+SLAFGTPPQ+LS +FDTGSSLVWFPCTAGYRCS 
Sbjct: 61  KTPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPG 180
           CSFP VD ATI KF+PKLSSS K++GCRN KC+WIFGPNLK+ CR+C+ +SRKCSD+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTCPG 180

Query: 181 YGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL 240
           YG+QYGSGATAG LLSETLD   KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLS 300
           KRFSHCLV R FDDSPVSSPLVLDS SES ESK  S IYAPFRENPS SNAAFREYYYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+S GNGGAIIDSGSTFTFLDKPIFEA+A+ELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 AKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXX 420
           AK VEA+SGLRPCF+I K EES EFP+++LKFKGG  L+L   NYLA+V D  VVCLT  
Sbjct: 361 AKGVEAESGLRPCFDISK-EESVEFPELILKFKGGATLALPPSNYLALVADTSVVCLTMI 420

Query: 421 XXXXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
                           GAFQQQNVLV+YDLAK+RIGFRKQ+CT
Sbjct: 421 TDVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of CsGy6G025400 vs. NCBI nr
Match: XP_022979057.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 653.7 bits (1685), Expect = 4.7e-184
Identity = 333/463 (71.92%), Postives = 365/463 (78.83%), Query Frame = 0


Query: 1   MEFLPIPFLFSI--FLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHL 60
           MEF PI FL SI                  LT FPS+  T P+K I  L+SASL RAQHL
Sbjct: 1   MEFFPIQFLLSIVXXXXXXXXXXXXXXXXXLTAFPSLPLTHPWKNIKHLVSASLARAQHL 60

Query: 61  KTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSR 120
           KTP++KSNTSIQNV+LFPRSYGAYS+SLAFGTPPQ+LS +FDTGSSLVWFPCTAGYRCS 
Sbjct: 61  KTPKTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPG 180
           CSFP VD ATI KF+PKLSSS +++GCRN KC+WIF                   D+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSARIIGCRNRKCSWIFXXXXXXXXXXXXXXXXXXXDTCPG 180

Query: 181 YGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL 240
           YG+QYGSGATAG LLSETLD   KRVPDFLVGCSV+SVHQPAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLS 300
           KRFSHCLV R FDDSPVSSPLVLDS  ES +SKT S IYAPFRENPS SNAAFREYYYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSPESGDSKTNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+S GNGGAIIDSGSTFTFLDKPIFEA+A+ELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 AKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXX 420
           AK VEA+SGLRPCF+I K EES EFP+++LKFKGG  L+L   NYLA+VTD GVVCLT  
Sbjct: 361 AKGVEAESGLRPCFDISK-EESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMI 420

Query: 421 XXXXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
                           GAFQQQNVLV+YDLAK+RIGFRKQ+CT
Sbjct: 421 TDVNFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of CsGy6G025400 vs. TAIR10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein)

HSP 1 Score: 449.9 bits (1156), Expect = 1.9e-126
Identity = 233/470 (49.57%), Postives = 306/470 (65.11%), Query Frame = 0


Query: 8   FLFSIFLLLPTSSSSSTTVLPLTTF--PSVSFTDPFKTINLLLSASLNRAQHLK------ 67
           F F IFL     S  S   LPL+ F     S  DP+ ++  L  +S+ RA  LK      
Sbjct: 7   FFFLIFL-----SVVSAVKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGTSIK 66

Query: 68  ------TPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAG 127
                 +  + ++ ++    L  +SYG YSVSL+FGTP Q + F+FDTGSSLVW PCT+ 
Sbjct: 67  PDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTSR 126

Query: 128 YRCSRCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCS 187
           Y CS C F  +DP  I +F+PK SSS K++GC++PKC +++GPN+  +CR C+  +R C+
Sbjct: 127 YLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTRNCT 186

Query: 188 DSCPGYGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLP 247
             CP Y LQYG G+TAG+L++E LD  +  VPDF+VGCS++S  QPAGIAGFGRGP SLP
Sbjct: 187 VGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPVSLP 246

Query: 248 SQMRLKRFSHCLVSRGFDDSPVSSPLVLDSGS-ESDESKTKSFIYAPFRENPSVSNAAFR 307
           SQM LKRFSHCLVSR FDD+ V++ L LD+GS  +  SKT    Y PFR+NP+VSN AF 
Sbjct: 247 SQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAFL 306

Query: 308 EYYYLSLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQ 367
           EYYYL+LRRI +G K VK PYKYL P + G+GG+I+DSGSTFTF+++P+FE +A+E   Q
Sbjct: 307 EYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFASQ 366

Query: 368 LVKYPRAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGV 427
           +  Y R KD+E ++GL PCFNI  + +    P+++ +FKGG KL L   NY   V +   
Sbjct: 367 MSNYTREKDLEKETGLGPCFNISGKGD-VTVPELIFEFKGGAKLELPLSNYFTFVGNTDT 426

Query: 428 VCLT-XXXXXXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           VCLT                   G+FQQQN LVEYDL   R GF K+KC+
Sbjct: 427 VCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

[Close]

BLAST of CsGy6G025400 vs. TAIR10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein)

HSP 1 Score: 185.7 bits (470), Expect = 6.6e-47
Identity = 131/403 (32.51%), Postives = 181/403 (44.91%), Query Frame = 0


Query: 82  YSVSLAFGTPPQNLSFIFDTGSSLVWFPC-TAGYRCSRC-SFPYVDPATISKFVPKLSSS 141
           Y ++L  GTPPQ +    DTGS L W PC    + C  C      D  + S F P  SS+
Sbjct: 83  YLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYDLKNNDLKSPSVFSPLHSST 142

Query: 142 VKVVGCRNPKCAWI------FGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA-TAGIL 201
                C +  C  I      F P   + C         C   CP +   YG G   +GIL
Sbjct: 143 SFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLKSTCVRPCPSFAYTYGEGGLISGIL 202

Query: 202 LSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL--KRFSHCLVSRGF 261
             + L    + VP F  GC   +  +P GIAGFGRG  SLPSQ+    K FSHC +   F
Sbjct: 203 TRDILKARTRDVPRFSFGCVTSTYREPIGIAGFGRGLLSLPSQLGFLEKGFSHCFLPFKF 262

Query: 262 DDSP-VSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILIGGK-- 321
            ++P +SSPL+L + + S  + T S  + P    P   N+     YY+ L  I IG    
Sbjct: 263 VNNPNISSPLILGASALS-INLTDSLQFTPMLNTPMYPNS-----YYIGLESITIGTNIT 322

Query: 322 PVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSG 381
           P + P      DS GNGG ++DSG+T+T L +P +  +   L+   + YPRA + E+++G
Sbjct: 323 PTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQLLTTLQ-STITYPRATETESRTG 382

Query: 382 LRPCFNIP---------KEEESAEFPDVVLKFKGGGKLSLAAEN-YLAMVTDEGVVCLTX 441
              C+ +P         + +    FP +   F     L L   N + AM        +  
Sbjct: 383 FDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNNATLLLPQGNSFYAMSAPSDGSVVQC 442

Query: 442 XXXXXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKC 461
                            G+FQQQNV V YDL K+RIGF+   C
Sbjct: 443 LLFQNMEDGDYGPAGVFGSFQQQNVKVVYDLEKERIGFQAMDC 478

[Close]

BLAST of CsGy6G025400 vs. TAIR10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein)

HSP 1 Score: 162.5 bits (410), Expect = 6.0e-40
Identity = 122/425 (28.71%), Postives = 186/425 (43.76%), Query Frame = 0


Query: 76  PRSYGA-YSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVP 135
           P S G+ Y +SL+ G+    +S   DTGS LVWFPC   + C  C    + P+       
Sbjct: 76  PISSGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILCESKPLPPSXXXXXXX 135

Query: 136 KLSSSVKVVGCRNPKCAWIFGPNL--KSRCRNCNSKSRKCSDS---CPGYGLQYGSGATA 195
                             +   +L   S C     ++  C+ S   CP +   YG G+  
Sbjct: 136 XXXXXXXXXXXXXXXXXXLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSLV 195

Query: 196 GILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL------KRFSH 255
             L S++L L +  V +F  GC+  ++ +P G+AGFGRG  SLP+Q+ +        FS+
Sbjct: 196 AKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFSY 255

Query: 256 CLVSRGFDDSPV--SSPLVLDSGSESDESKT----------------KSFIYAPFRENPS 315
           CLVS  FD   V   SPL+L    +  E +                   F++    ENP 
Sbjct: 256 CLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGXXXXXXXXXXXXXXXXEFVFTEMLENPK 315

Query: 316 VSNAAFREYYYLSLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAI 375
                   +Y +SL+ I IG + +  P      D  G GG ++DSG+TFT L    + ++
Sbjct: 316 -----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNSV 375

Query: 376 ADELEKQLVK-YPRAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGG-GKLSLAAENY 435
            +E + ++ + + RA  VE  SG+ PC+ +    ++ + P +VL F G    ++L   NY
Sbjct: 376 VEEFDSRVGRVHERADRVEPSSGMSPCYYL---NQTVKVPALVLHFAGNRSSVTLPRRNY 435

Query: 436 LAMVTDEG--------VVCLTXXXXXXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGF 461
                D G        + CL                   G +QQQ   V YDL  +R+GF
Sbjct: 436 FYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGF 491

[Close]

BLAST of CsGy6G025400 vs. TAIR10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein)

HSP 1 Score: 158.3 bits (399), Expect = 1.1e-38
Identity = 122/399 (30.58%), Postives = 181/399 (45.36%), Query Frame = 0


Query: 80  GAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSS 139
           G Y + +  GTPP++ S I DTGS L W  C   Y C   +  + D        PK S+S
Sbjct: 158 GEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGMFYD--------PKTSAS 217

Query: 140 VKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA-TAGILLSETLD 199
            K + C +P+C+ I  P+   +C + N        SCP Y   YG  + T G    ET  
Sbjct: 218 FKNITCNDPRCSLISSPDPPVQCESDN-------QSCP-YFYWYGDRSNTTGDFAVETFT 277

Query: 200 L---------ENKRVPDFLVGCSVMS---VHQPAGIAGFGRGPESLPSQMRL---KRFSH 259
           +            +V + + GC   +       +G+ G GRGP S  SQ++      FS+
Sbjct: 278 VNLTTTEGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 337

Query: 260 CLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRIL 319
           CLV R   ++ VSS L+   G + D     +  +  F      S   F   YY+ ++ IL
Sbjct: 338 CLVDRN-SNTNVSSKLIF--GEDKDLLNHTNLNFTSFVNGKENSVETF---YYIQIKSIL 397

Query: 320 IGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADEL-EKQLVKYPRAKDV 379
           +GGK +  P +     S G+GG IIDSG+T ++  +P +E I ++  EK    YP  +D 
Sbjct: 398 VGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDF 457

Query: 380 EAQSGLRPCFNIPK-EEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXXX 439
                L PCFN+   EE +   P++ + F  G   +  AEN    ++ E +VCL      
Sbjct: 458 PV---LDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLS-EDLVCLA----- 517

Query: 440 XXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKC 461
                        G +QQQN  + YD  + R+GF   KC
Sbjct: 518 -ILGTPKSTFSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

[Close]

BLAST of CsGy6G025400 vs. TAIR10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein)

HSP 1 Score: 151.0 bits (380), Expect = 1.8e-36
Identity = 117/400 (29.25%), Postives = 179/400 (44.75%), Query Frame = 0


Query: 80  GAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSS 139
           G Y + +  G+PP++ S I DTGS L W  C   Y C + +  + D        PK S+S
Sbjct: 168 GEYFMDVLVGSPPKHFSLILDTGSDLNWIQCLPCYDCFQQNGAFYD--------PKASAS 227

Query: 140 VKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGATAGILLSETLDL 199
            K + C + +C  +  P+    C++ N        SCP Y     S  T G    ET  +
Sbjct: 228 YKNITCNDQRCNLVSSPDPPMPCKSDN-------QSCPYYYWYGDSSNTTGDFAVETFTV 287

Query: 200 ---------ENKRVPDFLVGCSVMS---VHQPAGIAGFGRGPESLPSQMRL---KRFSHC 259
                    E   V + + GC   +    H  AG+ G GRGP S  SQ++      FS+C
Sbjct: 288 NLTTNGGSSELYNVENMMFGCGHWNRGLFHGAAGLLGLGRGPLSFSSQLQSLYGHSFSYC 347

Query: 260 LVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPF---RENPSVSNAAFREYYYLSLRR 319
           LV R   D+ VSS L+   G + D     +  +  F   +EN          +YY+ ++ 
Sbjct: 348 LVDRN-SDTNVSSKLIF--GEDKDLLSHPNLNFTSFVAGKEN------LVDTFYYVQIKS 407

Query: 320 ILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADEL-EKQLVKYPRAK 379
           IL+ G+ +  P +     S G GG IIDSG+T ++  +P +E I +++ EK   KYP  +
Sbjct: 408 ILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYR 467

Query: 380 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXX 439
           D      L PCFN+     + + P++ + F  G   +   EN    + +E +VCL     
Sbjct: 468 DFPI---LDPCFNV-SGIHNVQLPELGIAFADGAVWNFPTENSFIWL-NEDLVCLA---- 527

Query: 440 XXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKC 461
                         G +QQQN  + YD  + R+G+   KC
Sbjct: 528 --MLGTPKSAFSIIGNYQQQNFHILYDTKRSRLGYAPTKC 532

[Close]

BLAST of CsGy6G025400 vs. Swiss-Prot
Match: sp|Q940R4|ASP63_ARATH (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.1e-38
Identity = 122/425 (28.71%), Postives = 186/425 (43.76%), Query Frame = 0


Query: 76  PRSYGA-YSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVP 135
           P S G+ Y +SL+ G+    +S   DTGS LVWFPC   + C  C    + P+       
Sbjct: 76  PISSGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILCESKPLPPSXXXXXXX 135

Query: 136 KLSSSVKVVGCRNPKCAWIFGPNL--KSRCRNCNSKSRKCSDS---CPGYGLQYGSGATA 195
                             +   +L   S C     ++  C+ S   CP +   YG G+  
Sbjct: 136 XXXXXXXXXXXXXXXXXXLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSLV 195

Query: 196 GILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL------KRFSH 255
             L S++L L +  V +F  GC+  ++ +P G+AGFGRG  SLP+Q+ +        FS+
Sbjct: 196 AKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFSY 255

Query: 256 CLVSRGFDDSPV--SSPLVLDSGSESDESKT----------------KSFIYAPFRENPS 315
           CLVS  FD   V   SPL+L    +  E +                   F++    ENP 
Sbjct: 256 CLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGXXXXXXXXXXXXXXXXEFVFTEMLENPK 315

Query: 316 VSNAAFREYYYLSLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAI 375
                   +Y +SL+ I IG + +  P      D  G GG ++DSG+TFT L    + ++
Sbjct: 316 -----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNSV 375

Query: 376 ADELEKQLVK-YPRAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGG-GKLSLAAENY 435
            +E + ++ + + RA  VE  SG+ PC+ +    ++ + P +VL F G    ++L   NY
Sbjct: 376 VEEFDSRVGRVHERADRVEPSSGMSPCYYL---NQTVKVPALVLHFAGNRSSVTLPRRNY 435

Query: 436 LAMVTDEG--------VVCLTXXXXXXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGF 461
                D G        + CL                   G +QQQ   V YDL  +R+GF
Sbjct: 436 FYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGF 491

[Close]

BLAST of CsGy6G025400 vs. Swiss-Prot
Match: sp|Q766C2|NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.3e-36
Identity = 117/386 (30.31%), Postives = 175/386 (45.34%), Query Frame = 0


Query: 80  GAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSS 139
           G Y +++A GTP  + S I DTGS L+W  C     C++C   +  P  I  F P+ SSS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQC---FSQPTPI--FNPQDSSS 153

Query: 140 VKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGATA-GILLSETLD 199
              + C +  C               +  S  C+++   Y   YG G+T  G + +ET  
Sbjct: 154 FSTLPCESQYCQ--------------DLPSETCNNNECQYTYGYGDGSTTQGYMATETFT 213

Query: 200 LENKRVPDFLVGCSV----MSVHQPAGIAGFGRGPESLPSQMRLKRFSHCLVSRGFDDSP 259
            E   VP+   GC            AG+ G G GP SLPSQ+ + +FS+C+ S G   SP
Sbjct: 214 FETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYG-SSSP 273

Query: 260 VSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILIGGKPVKFPYK 319
            +  L   +    + S + + I++    NP+        YYY++L+ I +GG  +  P  
Sbjct: 274 STLALGSAASGVPEGSPSTTLIHSSL--NPT--------YYYITLQGITVGGDNLGIPSS 333

Query: 320 YLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSGLRPCFNI 379
                  G GG IIDSG+T T+L +  + A+A     Q +  P     E+ SGL  CF  
Sbjct: 334 TFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQ-INLPTVD--ESSSGLSTCFQQ 393

Query: 380 PKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXXXXXXXXXXXXXXXX 439
           P +  + + P++ ++F  GG L+L  +N L +   EGV+CL                   
Sbjct: 394 PSDGSTVQVPEISMQF-DGGVLNLGEQNIL-ISPAEGVICLA------MGSSSQLGISIF 435

Query: 440 GAFQQQNVLVEYDLAKQRIGFRKQKC 461
           G  QQQ   V YDL    + F   +C
Sbjct: 454 GNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of CsGy6G025400 vs. Swiss-Prot
Match: sp|Q766C3|NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 9.5e-35
Identity = 111/387 (28.68%), Postives = 168/387 (43.41%), Query Frame = 0


Query: 80  GAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSS 139
           G Y ++L+ GTP Q  S I DTGS L+W  C    +C   S P         F P+ SSS
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPI--------FNPQGSSS 152

Query: 140 VKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA-TAGILLSETLD 199
              + C +  C  +  P               CS++   Y   YG G+ T G + +ETL 
Sbjct: 153 FSTLPCSSQLCQALSSPT--------------CSNNFCQYTYGYGDGSETQGSMGTETLT 212

Query: 200 LENKRVPDFLVGCSV----MSVHQPAGIAGFGRGPESLPSQMRLKRFSHCLVSRGFDDSP 259
             +  +P+   GC            AG+ G GRGP SLPSQ+ + +FS+C+   G   S 
Sbjct: 213 FGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIG---SS 272

Query: 260 VSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILIGGKPVKF-PY 319
             S L+L S + S        + A       + ++    +YY++L  + +G   +   P 
Sbjct: 273 TPSNLLLGSLANS--------VTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPS 332

Query: 320 KYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSGLRPCFN 379
            + +  + G GG IIDSG+T T+     ++++  E   Q +  P      + SG   CF 
Sbjct: 333 AFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQ-INLPVVNG--SSSGFDLCFQ 392

Query: 380 IPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXXXXXXXXXXXXXXX 439
            P +  + + P  V+ F  GG L L +ENY  +    G++CL                  
Sbjct: 393 TPSDPSNLQIPTFVMHF-DGGDLELPSENYF-ISPSNGLICLA-------MGSSSQGMSI 434

Query: 440 XGAFQQQNVLVEYDLAKQRIGFRKQKC 461
            G  QQQN+LV YD     + F   +C
Sbjct: 453 FGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of CsGy6G025400 vs. Swiss-Prot
Match: sp|Q9LNJ3|APF2_ARATH (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 3.4e-32
Identity = 123/412 (29.85%), Postives = 169/412 (41.02%), Query Frame = 0


Query: 57  HLKTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRC 116
           H   P   S++ +  +S   +  G Y   L  GTP + +  + DTGS +VW  C    RC
Sbjct: 120 HAPRPGGFSSSVVSGLS---QGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC 179

Query: 117 SRCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSC 176
              S P  D        P+ S +   + C +P C        +     CN++ + C    
Sbjct: 180 YSQSDPIFD--------PRKSKTYATIPCSSPHCR-------RLDSAGCNTRRKTCL--- 239

Query: 177 PGYGLQYGSGA-TAGILLSETLDLENKRVPDFLVGCSVMS---VHQPAGIAGFGRGPESL 236
             Y + YG G+ T G   +ETL     RV    +GC   +       AG+ G G+G  S 
Sbjct: 240 --YQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSF 299

Query: 237 PSQMRLK---RFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNA 296
           P Q   +   +FS+CLV R     P  S +V  + + S  +          R  P +SN 
Sbjct: 300 PGQTGHRFNQKFSYCLVDRSASSKP--SSVVFGNAAVSRIA----------RFTPLLSNP 359

Query: 297 AFREYYYLSLRRILIGGKPVKFPYKYLVP-DSTGNGGAIIDSGSTFTFLDKPIFEAIADE 356
               +YY+ L  I +GG  V      L   D  GNGG IIDSG++ T L +P + A+ D 
Sbjct: 360 KLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDA 419

Query: 357 LEKQLVKYPRAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVT 416
                    RA D    S    CF++    E  + P VVL F+ G  +SL A NYL  V 
Sbjct: 420 FRVGAKTLKRAPDF---SLFDTCFDLSNMNE-VKVPTVVLHFR-GADVSLPATNYLIPVD 479

Query: 417 DEGVVCLTXXXXXXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKC 461
             G  C                    G  QQQ   V YDLA  R+GF    C
Sbjct: 480 TNGKFCFA-------FAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of CsGy6G025400 vs. Swiss-Prot
Match: sp|Q9LS40|ASPG1_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 9.2e-30
Identity = 113/386 (29.27%), Postives = 162/386 (41.97%), Query Frame = 0


Query: 80  GAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPATISKFVPKLSSS 139
           G Y   +  GTP + +  + DTGS + W  C     C+ C +   DP     F P  SS+
Sbjct: 160 GEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEP---CADC-YQQSDPV----FNPTSSST 219

Query: 140 VKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA-TAGILLSETLD 199
            K + C  P+C+ +      S CR     S KC      Y + YG G+ T G L ++T+ 
Sbjct: 220 YKSLTCSAPQCSLL----ETSACR-----SNKCL-----YQVSYGDGSFTVGELATDTVT 279

Query: 200 LENK-RVPDFLVGCSVMS---VHQPAGIAGFGRGPESLPSQMRLKRFSHCLVSRGFDDSP 259
             N  ++ +  +GC   +       AG+ G G G  S+ +QM+   FS+CLV R   DS 
Sbjct: 280 FGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDR---DSG 339

Query: 260 VSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILIGGKPVKFPYK 319
            SS L  +S        T     AP   N  +       +YY+ L    +GG+ V  P  
Sbjct: 340 KSSSLDFNSVQLGGGDAT-----APLLRNKKIDT-----FYYVGLSGFSVGGEKVVLPDA 399

Query: 320 YLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSGLRPCFNI 379
               D++G+GG I+D G+  T L    + ++ D   K  V     K   + S    C++ 
Sbjct: 400 IFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNL--KKGSSSISLFDTCYDF 459

Query: 380 PKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXXXXXXXXXXXXXXXX 439
                + + P V   F GG  L L A+NYL  V D G  C                    
Sbjct: 460 -SSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFA-------FAPTSSSLSII 500

Query: 440 GAFQQQNVLVEYDLAKQRIGFRKQKC 461
           G  QQQ   + YDL+K  IG    KC
Sbjct: 520 GNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of CsGy6G025400 vs. TrEMBL
Match: tr|A0A0A0KHK2|A0A0A0KHK2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454470 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 3.2e-253
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS
Sbjct: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG
Sbjct: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR
Sbjct: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXX 420
           DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXX
Sbjct: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXX 420

Query: 421 XXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           XXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 XXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 461

[Close]

BLAST of CsGy6G025400 vs. TrEMBL
Match: tr|A0A1S3CHV2|A0A1S3CHV2_CUCME (aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 PE=3 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 3.1e-232
Identity = 405/461 (87.85%), Postives = 421/461 (91.32%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKT 60
           MEFLPIPFLFSIFLLLPTSSSSS T LPL TFPS+ FTDP KTIN LLSASL+RAQHLK+
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSIT-LPLATFPSIPFTDPLKTINHLLSASLSRAQHLKS 60

Query: 61  PQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCS 120
           PQSKSNTS +NVSLFPRSYGAY+VSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRC+ CS
Sbjct: 61  PQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAHCS 120

Query: 121 FPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYG 180
           FP+VDPATISKFVPKLSSSVK+VGCRNPKCAWIFGPNLKSRCRNCN KSRKCSDSCPGYG
Sbjct: 121 FPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPGYG 180

Query: 181 LQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240
           +QYGSGATAGILLSETLDL+NKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR
Sbjct: 181 IQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKR 240

Query: 241 FSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLR 300
           FSHCL+ RGFDDSPVSSPLVLDSG ESDESKTKSFIYAPF+ENPS SN AFREYYYLSLR
Sbjct: 241 FSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLSLR 300

Query: 301 RILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAK 360
           RILIGGKPVKFPYKYLVPDSTG GGAIIDSGSTFTFLDKPIFEAIA ELEKQLVKYPRAK
Sbjct: 301 RILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPRAK 360

Query: 361 DVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXX 420
           D+EA++GLRPCFNI KEEESAEFP+V LKFKGGGKLSL  ENYL MVTD  VVCLT    
Sbjct: 361 DIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMMTN 420

Query: 421 XXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
                         GAFQQQNVLVEYDLAKQRIGFRKQKCT
Sbjct: 421 AEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of CsGy6G025400 vs. TrEMBL
Match: tr|A0A1S3B6B5|A0A1S3B6B5_CUCME (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103486666 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 1.5e-146
Identity = 273/454 (60.13%), Postives = 339/454 (74.67%), Query Frame = 0


Query: 8   FLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKTPQSKSNT 67
           F   +F  L   S+S+   LPL + P +S +DP + +  L SAS NRA  +KTP+S    
Sbjct: 10  FYILLFSSLSAISNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAHRIKTPKS---N 69

Query: 68  SIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPA 127
           S+    L P SYGAYS  L+FGTP Q L  IFDTGSSLVWFPCT+ Y C+ CSFP +DP 
Sbjct: 70  SVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTECSFPKIDPT 129

Query: 128 TISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA 187
            I +FVPKLSSS K+VGC+NPKCAWIFGP++KS+CR+CN K+  C+ +CP Y +QYGSG+
Sbjct: 130 GIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAYVVQYGSGS 189

Query: 188 TAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKRFSHCLVS 247
           TAG+LLSETLD  NK++P+F+VGCS +S+HQP+GIAGFGRG ESLPSQM LK+F++CL S
Sbjct: 190 TAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLAS 249

Query: 248 RGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILIGGK 307
           R FDDS  S  L+LDS       KT    Y  FR+NPSVSN A++EYYYL++R+I++G +
Sbjct: 250 RKFDDSAHSGQLILDSSG----VKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKIIVGNQ 309

Query: 308 PVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSG 367
            VK PYKYLVP   GNGG+IIDSGSTFTF+DKP+ + +A E EKQL    RA DVE  +G
Sbjct: 310 AVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVETLTG 369

Query: 368 LRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTXXXXXXXXXXX 427
           LRPCF++ K E+S EFP+++ +FKGG K +L   NY A+V+  GV CL XXXXXXXXXXX
Sbjct: 370 LRPCFDVSK-EKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLXXXXXXXXXXXX 429

Query: 428 XXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           XXXXXX GAFQQQN  VEYDL  +R+GFRKQ CT
Sbjct: 430 XXXXXXLGAFQQQNFYVEYDLVNERLGFRKQTCT 455

[Close]

BLAST of CsGy6G025400 vs. TrEMBL
Match: tr|A0A2P6RCE3|A0A2P6RCE3_ROSCH (Putative nepenthesin OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0475521 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 1.9e-141
Identity = 256/455 (56.26%), Postives = 330/455 (72.53%), Query Frame = 0


Query: 9   LFSIFLLLPTSSSSSTTVLPLTTFPS-VSFTDPFKTINLLLSASLNRAQHLKTPQSKSNT 68
           L S+F L   + SS  T LPL+   +  S +DP +T++LL SASL+RA HLK P+  SN+
Sbjct: 11  LISLFSLFHLAFSSKLT-LPLSLLATHQSSSDPIQTVSLLSSASLSRAHHLKRPK-HSNS 70

Query: 69  SIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPA 128
           S   V L+PRS+G YS+ L+FGTPPQ  +F+ DTGSSLVWFPCT+ Y CS C FP +DP 
Sbjct: 71  STTKVPLYPRSFGGYSIPLSFGTPPQTSTFVMDTGSSLVWFPCTSRYSCSSCVFPNIDPT 130

Query: 129 TISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA 188
            I  F+P+LSS+ ++VGC+NPKCAWIFGP + ++C N        S +CP Y +QYGSGA
Sbjct: 131 HIPTFIPRLSSTSRIVGCKNPKCAWIFGPQINTKCPN-------SSQACPTYRIQYGSGA 190

Query: 189 TAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKRFSHCLVS 248
           TAG+LLSE+LDL NK VPDFLVGCS++S+ QPAGIAGFGRG ESLP QM L +FS+CLVS
Sbjct: 191 TAGVLLSESLDLPNKTVPDFLVGCSILSIRQPAGIAGFGRGQESLPVQMGLSKFSYCLVS 250

Query: 249 RGFDDSPVSSPLVLDSGSESDESKTKSFI-YAPFRENPSVSNAAFREYYYLSLRRILIGG 308
           R FDD+PVSS LVL SGS SD+      + Y PF++NP  SN+A+REYYYL+LR++++G 
Sbjct: 251 RRFDDTPVSSDLVLYSGSTSDDHDDSGGVSYTPFQKNPGASNSAYREYYYLALRKVVVGK 310

Query: 309 KPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQS 368
           K VK PYKYLVP +  NGG I+DSGSTFTF+++P+FEA+A+    Q+V+Y RAKD+E  +
Sbjct: 311 KHVKIPYKYLVPGADDNGGTIVDSGSTFTFMERPVFEAVAEAFAAQMVQYTRAKDIENGT 370

Query: 369 GLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLT-XXXXXXXXX 428
           GL+PCF+I K E++ +FP++V +FKGG K++L   NY A+VT  GVVCLT          
Sbjct: 371 GLKPCFDISK-EKTVDFPELVFQFKGGAKMALPLSNYFALVTSGGVVCLTIVTDGVAGPS 430

Query: 429 XXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKC 461
                    G+FQQQN  VEYDLA  R GFR+Q C
Sbjct: 431 LPSGPAIILGSFQQQNFYVEYDLAHDRFGFRQQSC 455

[Close]

BLAST of CsGy6G025400 vs. TrEMBL
Match: tr|A0A0A0LBI9|A0A0A0LBI9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778440 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 5.6e-141
Identity = 271/462 (58.66%), Postives = 341/462 (73.81%), Query Frame = 0


Query: 5   PIPFLFSIFLLLPTSSS---SSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKTP 64
           P P  F   LL  + S+   S+   LPL +FP +S  DP + +  L S+S  RA  +KTP
Sbjct: 4   PSPLSFFYLLLFSSLSAIAHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAHQIKTP 63

Query: 65  QSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSF 124
           +S    S+    L P SYGAYS  L+FGTP Q L  IFDTGSSLVWFPCT+ Y CS CSF
Sbjct: 64  KS---NSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSECSF 123

Query: 125 PYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGL 184
           P +DP  I +FVPKLSSS K+VGC+NPKC+WIFGP++KS+CR+CN K+  C+ +CP Y +
Sbjct: 124 PKIDPTGIPRFVPKLSSSSKLVGCQNPKCSWIFGPDVKSQCRSCNPKTENCTQTCPAYVV 183

Query: 185 QYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKRF 244
           QYGSG+TAG+LLSETLD  +K++P+F+VGCS +S+HQP+GIAGFGRG ESLPSQM LK+F
Sbjct: 184 QYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKF 243

Query: 245 SHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRR 304
           ++CL SR FDDSP S  L+LDS       K+    Y PFR+NPSVSN A++EYYYL++R+
Sbjct: 244 AYCLASRKFDDSPHSGQLILDSTG----VKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRK 303

Query: 305 ILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKD 364
           I++G + VK PYK+LVP   GNGG+IIDSGSTFTF+DKP+ E +A E EKQL  + RA D
Sbjct: 304 IIVGNQAVKVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATD 363

Query: 365 VEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGV--VCLTXXX 424
           VE  +GLRPCF+I K E+S +FP+++ +FKGG K +L   NY A+V+  GV      XXX
Sbjct: 364 VETLTGLRPCFDISK-EKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVXXXXXXXXX 423

Query: 425 XXXXXXXXXXXXXXXGAFQQQNVLVEYDLAKQRIGFRKQKCT 462
           XXXXXXXXXXXXXXX AFQQQN  VEYDL  QR+GFR+Q C+
Sbjct: 424 XXXXXXXXXXXXXXXXAFQQQNFYVEYDLVNQRLGFRQQTCS 457

The following BLAST results are available for this feature:

BLAST of CsGy6G025400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011657732.1	4.8e-253	100.00	PREDICTED: aspartic proteinase nepenthesin-2-like [Cucumis sativus] >KGN48299.1 ...	[more]
XP_008462617.1	4.7e-232	87.85	PREDICTED: aspartic proteinase nepenthesin-2-like [Cucumis melo]	[more]
XP_023543736.1	4.4e-198	75.81	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
XP_022925946.1	5.4e-196	74.95	probable aspartyl protease At4g16563 [Cucurbita moschata]	[more]
XP_022979057.1	4.7e-184	71.92	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]

BLAST of CsGy6G025400 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G52500.1	1.9e-126	49.57	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	6.6e-47	32.51	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	6.0e-40	28.71	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	1.1e-38	30.58	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.1	1.8e-36	29.25	Eukaryotic aspartyl protease family protein	[more]

BLAST of CsGy6G025400 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q940R4\|ASP63_ARATH	1.1e-38	28.71	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
sp\|Q766C2\|NEP2_NEPGR	1.3e-36	30.31	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
sp\|Q766C3\|NEP1_NEPGR	9.5e-35	28.68	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
sp\|Q9LNJ3\|APF2_ARATH	3.4e-32	29.85	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
sp\|Q9LS40\|ASPG1_ARATH	9.2e-30	29.27	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of CsGy6G025400 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KHK2\|A0A0A0KHK2_CUCSA	3.2e-253	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454470 PE=3 SV=1	[more]
tr\|A0A1S3CHV2\|A0A1S3CHV2_CUCME	3.1e-232	87.85	aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 P...	[more]
tr\|A0A1S3B6B5\|A0A1S3B6B5_CUCME	1.5e-146	60.13	aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103486666 PE=3 S...	[more]
tr\|A0A2P6RCE3\|A0A2P6RCE3_ROSCH	1.9e-141	56.26	Putative nepenthesin OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0475521 PE=3 SV...	[more]
tr\|A0A0A0LBI9\|A0A0A0LBI9_CUCSA	5.6e-141	58.66	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778440 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004190	aspartic-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR034161	Pepsin-like_plant
IPR033121	PEPTIDASE_A1
IPR001969	Aspartic_peptidase_AS
IPR032861	TAXi_N
IPR021109	Peptidase_aspartic_dom_sf
IPR032799	TAXi_C
IPR001461	Aspartic_peptidase_A1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004190	aspartic-type endopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy6G025400.1	CsGy6G025400.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 88..108 score: 51.84 coord: 326..337 score: 36.31
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 9..460
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 295..456 e-value: 6.8E-38 score: 129.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	G3DSA:2.40.70.10		coord: 264..461 e-value: 5.0E-52 score: 178.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	G3DSA:2.40.70.10		coord: 63..261 e-value: 4.7E-33 score: 116.9
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	SSF50630	Acid proteases	coord: 76..460
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 82..249 e-value: 8.2E-29 score: 101.0
None	No IPR available	PANTHER	PTHR13683:SF340	SUBFAMILY NOT NAMED	coord: 9..460
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 97..108
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 82..456 score: 35.155
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 81..460 e-value: 1.19889E-78 score: 247.176

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsGy6G025400	Cla008397	Watermelon (97103) v1	cgybwmB469
CsGy6G025400	Csa3G778440	Cucumber (Chinese Long) v2	cgybcuB255
CsGy6G025400	Csa6G454470	Cucumber (Chinese Long) v2	cgybcuB281
CsGy6G025400	MELO3C024582	Melon (DHL92) v3.5.1	cgybmeB446
CsGy6G025400	ClCG01G014950	Watermelon (Charleston Gray)	cgybwcgB399
CsGy6G025400	CSPI03G35440	Wild cucumber (PI 183967)	cgybcpiB262
CsGy6G025400	CSPI06G25830	Wild cucumber (PI 183967)	cgybcpiB287
CsGy6G025400	CmaCh14G003480	Cucurbita maxima (Rimu)	cgybcmaB731
CsGy6G025400	CmaCh03G011230	Cucurbita maxima (Rimu)	cgybcmaB831
CsGy6G025400	CmoCh03G011130	Cucurbita moschata (Rifu)	cgybcmoB811
CsGy6G025400	CmoCh14G003440	Cucurbita moschata (Rifu)	cgybcmoB713
CsGy6G025400	Lsi01G011870	Bottle gourd (USVL1VR-Ls)	cgyblsiB398
CsGy6G025400	Cp4.1LG10g03780	Cucurbita pepo (Zucchini)	cgybcpeB710
CsGy6G025400	Cp4.1LG03g00550	Cucurbita pepo (Zucchini)	cgybcpeB829
CsGy6G025400	MELO3C024582.2	Melon (DHL92) v3.6.1	cgybmedB443
CsGy6G025400	CsaV3_3G037790	Cucumber (Chinese Long) v3	cgybcucB268
CsGy6G025400	CsaV3_6G041050	Cucumber (Chinese Long) v3	cgybcucB298
CsGy6G025400	Cla97C01G013980	Watermelon (97103) v2	cgybwmbB394
CsGy6G025400	Bhi03G001383	Wax gourd	cgybwgoB601
CsGy6G025400	Carg10511	Silver-seed gourd	carcgybB0255

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy6G025400	Cucumber (Gy14) v2	cgybcgybB100
CsGy6G025400	Cucurbita maxima (Rimu)	cgybcmaB851
CsGy6G025400	Cucurbita maxima (Rimu)	cgybcmaB861
CsGy6G025400	Cucurbita moschata (Rifu)	cgybcmoB833
CsGy6G025400	Cucurbita moschata (Rifu)	cgybcmoB843
CsGy6G025400	Cucurbita pepo (Zucchini)	cgybcpeB810
CsGy6G025400	Cucurbita pepo (Zucchini)	cgybcpeB888
CsGy6G025400	Bottle gourd (USVL1VR-Ls)	cgyblsiB410
CsGy6G025400	Melon (DHL92) v3.5.1	cgybmeB424
CsGy6G025400	Melon (DHL92) v3.6.1	cgybmedB420
CsGy6G025400	Watermelon (Charleston Gray)	cgybwcgB392
CsGy6G025400	Watermelon (97103) v1	cgybwmB423
CsGy6G025400	Silver-seed gourd	carcgybB0266
CsGy6G025400	Silver-seed gourd	carcgybB0799
CsGy6G025400	Watermelon (97103) v2	cgybwmbB398
CsGy6G025400	Wax gourd	cgybwgoB503
CsGy6G025400	Wax gourd	cgybwgoB507