CsGy6G024310 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy6G024310
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	Glutamate-ammonia ligases,catalytics,glutamate-ammonia ligases isoform 2
Location	Chr6 : 24194939 .. 24195775 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTGAGTAATAATTATGAGAATGGTGGAGTTAATGGAATGAAAGTTGTACCAAATGAAGTGAAGCTTGTTCGTATTATTTGGGTTGATGCCTCTGGACACCATAGATGTCGTGTGAGATTTTATTTTATTTTCAGAATCTATTTATTTAATTTAGAATCTTTTTTGTAGATTAAATTCTCCGTATACATTTTGATTTTAATTTTCATTAAGTTCGTAATATTGAATTTAACTTCAATTTAATTTGCATTCTTTCATAACCTTACAAATTTTAGATGTTCATAAATTTTGATGTTTGTTACATGCATTTAGGTTTGAGTTTTTATTTCTATTTTTACTAAACACTCACTTTCACATTTAACTATTTATTTTTTAGGTTTAATGTTATATGTATTATTTTGAATTTATTAATTCTAGTTACTTTGATTAGTTAAATTAGCAAAATTTTAAAATCTACAAACACATAGTTGAATTATGCTTATGTTAGTTATTCTTTTTATCTTTTATGTGTATACGTTCGAATGAAGTTTCTTAAATGTTGTGTCCTTAATCATCATCATTATTTGTTAGTTTTTTTATTAACCTTGTTTGATTAATTATATAATGTTGTTACGATTGTAGGTTATTCCATCAACGCGATTCAATGATGTGGTTAAAAAGAATGGTGTTGGTTTGTCTGTTGTATGTATGGCAAGATCTTCTTATACCGATGTTCCAATTGGAAAAAATCTTACGGGTGCTGGTGAGATCAGATTGCTGCCAGATTTATCAACCAAACGAATAGTTCCTTGGTATTTTTGTATTTCAACCATATACTTACGATGTCGTCAATAA

mRNA sequence

ATGGATTTGAGTAATAATTATGAGAATGGTGGAGTTAATGGAATGAAAGTTGTACCAAATGAAGTGAAGCTTGTTCGTATTATTTGGGTTGATGCCTCTGGACACCATAGATGTCGTGTTATTCCATCAACGCGATTCAATGATGTGGTTAAAAAGAATGGTGTTGGTTTGTCTGTTGTATGTATGGCAAGATCTTCTTATACCGATGTTCCAATTGGAAAAAATCTTACGGGTGCTGGTGAGATCAGATTGCTGCCAGATTTATCAACCAAACGAATAGTTCCTTGGTATTTTTGTATTTCAACCATATACTTACGATGTCGTCAATAA

Coding sequence (CDS)

ATGGATTTGAGTAATAATTATGAGAATGGTGGAGTTAATGGAATGAAAGTTGTACCAAATGAAGTGAAGCTTGTTCGTATTATTTGGGTTGATGCCTCTGGACACCATAGATGTCGTGTTATTCCATCAACGCGATTCAATGATGTGGTTAAAAAGAATGGTGTTGGTTTGTCTGTTGTATGTATGGCAAGATCTTCTTATACCGATGTTCCAATTGGAAAAAATCTTACGGGTGCTGGTGAGATCAGATTGCTGCCAGATTTATCAACCAAACGAATAGTTCCTTGGTATTTTTGTATTTCAACCATATACTTACGATGTCGTCAATAA

Protein sequence

MDLSNNYENGGVNGMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPWYFCISTIYLRCRQ

Homology

[Close]

BLAST of CsGy6G024310 vs. NCBI nr
Match: XP_011657676.1 (PREDICTED: type-1 glutamine synthetase 1-like [Cucumis sativus] >KGN48205.1 hypothetical protein Csa_6G448140 [Cucumis sativus])

HSP 1 Score: 204.1 bits (518), Expect = 2.4e-49
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 0


Query: 1  MDLSNNYENGGVNGMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVV 60
          MDLSNNYENGGVNGMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVV
Sbjct: 1  MDLSNNYENGGVNGMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVV 60

Query: 61 CMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW 97
          CMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW
Sbjct: 61 CMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW 96

[Close]

BLAST of CsGy6G024310 vs. NCBI nr
Match: XP_008462507.1 (PREDICTED: type-1 glutamine synthetase 1-like [Cucumis melo])

HSP 1 Score: 169.9 bits (429), Expect = 4.9e-39
Identity = 85/97 (87.63%), Postives = 89/97 (91.75%), Query Frame = 0


Query: 1  MDLSNNYENGGVNGMK-VVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSV 60
          MDLS N ENGGV+ +K VV  EVKLVRIIWVDASG HRCRVIPSTRFNDVVKKNG+GLSV
Sbjct: 1  MDLS-NCENGGVHEVKVVVSKEVKLVRIIWVDASGQHRCRVIPSTRFNDVVKKNGIGLSV 60

Query: 61 VCMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW 97
          VCMARSS+TDVPIGKNLTGAGEIRLLPDLSTKR VPW
Sbjct: 61 VCMARSSHTDVPIGKNLTGAGEIRLLPDLSTKRTVPW 96

[Close]

BLAST of CsGy6G024310 vs. NCBI nr
Match: XP_011657677.1 (PREDICTED: type-1 glutamine synthetase 1-like isoform X1 [Cucumis sativus] >KGN48206.1 hypothetical protein Csa_6G448150 [Cucumis sativus])

HSP 1 Score: 163.3 bits (412), Expect = 4.6e-37
Identity = 78/96 (81.25%), Postives = 84/96 (87.50%), Query Frame = 0


Query: 1  MDLSNNYENGGVNGMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVV 60
          MDLSNN ENGGV       N+ K+VRIIWVD SG HRCRVIPSTRFNDVVKKNGVGLSVV
Sbjct: 1  MDLSNNCENGGV-------NQTKVVRIIWVDTSGQHRCRVIPSTRFNDVVKKNGVGLSVV 60

Query: 61 CMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW 97
          CMARSS+T++PIGK+LTG GEIRLLPDLSTKRIVPW
Sbjct: 61 CMARSSHTEIPIGKDLTGVGEIRLLPDLSTKRIVPW 89

[Close]

BLAST of CsGy6G024310 vs. NCBI nr
Match: XP_011657678.1 (PREDICTED: type-1 glutamine synthetase 1-like isoform X2 [Cucumis sativus])

HSP 1 Score: 124.8 bits (312), Expect = 1.8e-25
Identity = 64/96 (66.67%), Postives = 70/96 (72.92%), Query Frame = 0


Query: 1  MDLSNNYENGGVNGMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVV 60
          MDLSNN ENGGVN                       + +VIPSTRFNDVVKKNGVGLSVV
Sbjct: 1  MDLSNNCENGGVN-----------------------QTKVIPSTRFNDVVKKNGVGLSVV 60

Query: 61 CMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW 97
          CMARSS+T++PIGK+LTG GEIRLLPDLSTKRIVPW
Sbjct: 61 CMARSSHTEIPIGKDLTGVGEIRLLPDLSTKRIVPW 73

[Close]

BLAST of CsGy6G024310 vs. NCBI nr
Match: XP_022143778.1 (protein fluG [Momordica charantia])

HSP 1 Score: 123.2 bits (308), Expect = 5.3e-25
Identity = 57/81 (70.37%), Postives = 65/81 (80.25%), Query Frame = 0


Query: 17  VVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSYTDVPI-GKN 76
           VV  + +LVRIIWVDASG HRCRV+PS RFNDVV+KNGVGL+  CM  +S+ D P  G N
Sbjct: 402 VVQKDARLVRIIWVDASGQHRCRVVPSERFNDVVEKNGVGLTFACMGMTSHLDGPADGSN 461

Query: 77  LTGAGEIRLLPDLSTKRIVPW 97
           LTG GEIRLLPDLSTK +VPW
Sbjct: 462 LTGVGEIRLLPDLSTKWMVPW 482

[Close]

BLAST of CsGy6G024310 vs. TAIR10
Match: AT3G53180.1 (glutamate-ammonia ligases;catalytics;glutamate-ammonia ligases)

HSP 1 Score: 95.9 bits (237), Expect = 1.6e-20
Identity = 44/73 (60.27%), Postives = 54/73 (73.97%), Query Frame = 0


Query: 25  VRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSYTDVPIGKN-LTGAGEIR 84
           VRIIWVD SG  RCR + + RFN  VKKNGVGL+   M  +S+TD P  ++ LTG GEIR
Sbjct: 422 VRIIWVDTSGQQRCRAVQAQRFNRSVKKNGVGLTFASMGMTSFTDGPAEESKLTGVGEIR 481

Query: 85  LLPDLSTKRIVPW 97
           L+PDLSTK+ +PW
Sbjct: 482 LVPDLSTKQTIPW 494

[Close]

BLAST of CsGy6G024310 vs. TrEMBL
Match: tr|A0A0A0KES4|A0A0A0KES4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448140 PE=3 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.6e-49
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 0


Query: 1  MDLSNNYENGGVNGMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVV 60
          MDLSNNYENGGVNGMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVV
Sbjct: 1  MDLSNNYENGGVNGMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVV 60

Query: 61 CMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW 97
          CMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW
Sbjct: 61 CMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW 96

[Close]

BLAST of CsGy6G024310 vs. TrEMBL
Match: tr|A0A1S3CIM4|A0A1S3CIM4_CUCME (type-1 glutamine synthetase 1-like OS=Cucumis melo OX=3656 GN=LOC103500842 PE=3 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 3.3e-39
Identity = 85/97 (87.63%), Postives = 89/97 (91.75%), Query Frame = 0


Query: 1  MDLSNNYENGGVNGMK-VVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSV 60
          MDLS N ENGGV+ +K VV  EVKLVRIIWVDASG HRCRVIPSTRFNDVVKKNG+GLSV
Sbjct: 1  MDLS-NCENGGVHEVKVVVSKEVKLVRIIWVDASGQHRCRVIPSTRFNDVVKKNGIGLSV 60

Query: 61 VCMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW 97
          VCMARSS+TDVPIGKNLTGAGEIRLLPDLSTKR VPW
Sbjct: 61 VCMARSSHTDVPIGKNLTGAGEIRLLPDLSTKRTVPW 96

[Close]

BLAST of CsGy6G024310 vs. TrEMBL
Match: tr|A0A0A0KKB4|A0A0A0KKB4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448150 PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 3.0e-37
Identity = 78/96 (81.25%), Postives = 84/96 (87.50%), Query Frame = 0


Query: 1  MDLSNNYENGGVNGMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVV 60
          MDLSNN ENGGV       N+ K+VRIIWVD SG HRCRVIPSTRFNDVVKKNGVGLSVV
Sbjct: 1  MDLSNNCENGGV-------NQTKVVRIIWVDTSGQHRCRVIPSTRFNDVVKKNGVGLSVV 60

Query: 61 CMARSSYTDVPIGKNLTGAGEIRLLPDLSTKRIVPW 97
          CMARSS+T++PIGK+LTG GEIRLLPDLSTKRIVPW
Sbjct: 61 CMARSSHTEIPIGKDLTGVGEIRLLPDLSTKRIVPW 89

[Close]

BLAST of CsGy6G024310 vs. TrEMBL
Match: tr|A0A1R3IGZ1|A0A1R3IGZ1_COCAP (Amidohydrolase 2 OS=Corchorus capsularis OX=210143 GN=CCACVL1_12176 PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.5e-23
Identity = 53/84 (63.10%), Postives = 64/84 (76.19%), Query Frame = 0


Query: 14  GMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSYTDVPI- 73
           G  V  + V LVR+IWVDASG HRCRV+P  RF+DVVKKNGVGL+   MA +S+ D P  
Sbjct: 409 GTNVPEHSVSLVRVIWVDASGQHRCRVVPKKRFDDVVKKNGVGLTFASMAMTSFVDGPAD 468

Query: 74  GKNLTGAGEIRLLPDLSTKRIVPW 97
           G NLTG GEIRL+PDLST++ +PW
Sbjct: 469 GTNLTGTGEIRLMPDLSTRKAIPW 492

[Close]

BLAST of CsGy6G024310 vs. TrEMBL
Match: tr|A0A1R3HTI5|A0A1R3HTI5_9ROSI (Amidohydrolase 2 OS=Corchorus olitorius OX=93759 GN=COLO4_26904 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.5e-23
Identity = 52/84 (61.90%), Postives = 64/84 (76.19%), Query Frame = 0


Query: 14  GMKVVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSYTDVPI- 73
           G  V  + + LVR+IWVDASG HRCRV+P  RF+DVVKKNGVGL+   MA +S+ D P  
Sbjct: 341 GTNVPEHAISLVRVIWVDASGQHRCRVVPKKRFDDVVKKNGVGLTFASMAMTSFVDGPAD 400

Query: 74  GKNLTGAGEIRLLPDLSTKRIVPW 97
           G NLTG GEIRL+PDLST++ +PW
Sbjct: 401 GTNLTGTGEIRLMPDLSTRKAIPW 424

The following BLAST results are available for this feature:

BLAST of CsGy6G024310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011657676.1	2.4e-49	100.00	PREDICTED: type-1 glutamine synthetase 1-like [Cucumis sativus] >KGN48205.1 hypo...	[more]
XP_008462507.1	4.9e-39	87.63	PREDICTED: type-1 glutamine synthetase 1-like [Cucumis melo]	[more]
XP_011657677.1	4.6e-37	81.25	PREDICTED: type-1 glutamine synthetase 1-like isoform X1 [Cucumis sativus] >KGN4...	[more]
XP_011657678.1	1.8e-25	66.67	PREDICTED: type-1 glutamine synthetase 1-like isoform X2 [Cucumis sativus]	[more]
XP_022143778.1	5.3e-25	70.37	protein fluG [Momordica charantia]	[more]

BLAST of CsGy6G024310 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G53180.1	1.6e-20	60.27	glutamate-ammonia ligases;catalytics;glutamate-ammonia ligases	[more]

BLAST of CsGy6G024310 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CsGy6G024310 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KES4\|A0A0A0KES4_CUCSA	1.6e-49	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448140 PE=3 SV=1	[more]
tr\|A0A1S3CIM4\|A0A1S3CIM4_CUCME	3.3e-39	87.63	type-1 glutamine synthetase 1-like OS=Cucumis melo OX=3656 GN=LOC103500842 PE=3 ...	[more]
tr\|A0A0A0KKB4\|A0A0A0KKB4_CUCSA	3.0e-37	81.25	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448150 PE=3 SV=1	[more]
tr\|A0A1R3IGZ1\|A0A1R3IGZ1_COCAP	1.5e-23	63.10	Amidohydrolase 2 OS=Corchorus capsularis OX=210143 GN=CCACVL1_12176 PE=3 SV=1	[more]
tr\|A0A1R3HTI5\|A0A1R3HTI5_9ROSI	2.5e-23	61.90	Amidohydrolase 2 OS=Corchorus olitorius OX=93759 GN=COLO4_26904 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004356	glutamate-ammonia ligase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006807	nitrogen compound metabolic process
GO:0006542	glutamine biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR036651	Gln_synt_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006542	glutamine biosynthetic process
biological_process	GO:0006807	nitrogen compound metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004356	glutamate-ammonia ligase activity
molecular_function	GO:0016787	hydrolase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy6G024310.1	CsGy6G024310.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036651	Glutamine synthetase, N-terminal domain superfamily	GENE3D	G3DSA:3.10.20.70		coord: 17..105 e-value: 4.3E-8 score: 34.9
IPR036651	Glutamine synthetase, N-terminal domain superfamily	SUPERFAMILY	SSF54368	Glutamine synthetase, N-terminal domain	coord: 19..98
None	No IPR available	PANTHER	PTHR43785	FAMILY NOT NAMED	coord: 20..96
None	No IPR available	PANTHER	PTHR43785:SF2	TYPE-1 GLUTAMINE SYNTHETASE 1	coord: 20..96

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsGy6G024310	MELO3C024485	Melon (DHL92) v3.5.1	cgybmeB446
CsGy6G024310	Lsi01G012890	Bottle gourd (USVL1VR-Ls)	cgyblsiB398

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy6G024310	Cucumber (Gy14) v2	cgybcgybB100
CsGy6G024310	Cucumber (Gy14) v2	cgybcgybB151
CsGy6G024310	Cucurbita maxima (Rimu)	cgybcmaB729
CsGy6G024310	Cucurbita maxima (Rimu)	cgybcmaB795
CsGy6G024310	Cucurbita maxima (Rimu)	cgybcmaB831
CsGy6G024310	Cucurbita maxima (Rimu)	cgybcmaB861
CsGy6G024310	Cucurbita moschata (Rifu)	cgybcmoB712
CsGy6G024310	Cucurbita moschata (Rifu)	cgybcmoB773
CsGy6G024310	Cucurbita moschata (Rifu)	cgybcmoB811
CsGy6G024310	Cucurbita moschata (Rifu)	cgybcmoB843
CsGy6G024310	Cucurbita pepo (Zucchini)	cgybcpeB710
CsGy6G024310	Cucurbita pepo (Zucchini)	cgybcpeB773
CsGy6G024310	Cucurbita pepo (Zucchini)	cgybcpeB810
CsGy6G024310	Cucurbita pepo (Zucchini)	cgybcpeB826
CsGy6G024310	Cucumber (Chinese Long) v2	cgybcuB254
CsGy6G024310	Cucumber (Chinese Long) v2	cgybcuB281
CsGy6G024310	Cucumber (Chinese Long) v2	cgybcuB283
CsGy6G024310	Bottle gourd (USVL1VR-Ls)	cgyblsiB384
CsGy6G024310	Melon (DHL92) v3.5.1	cgybmeB373
CsGy6G024310	Melon (DHL92) v3.6.1	cgybmedB374
CsGy6G024310	Melon (DHL92) v3.6.1	cgybmedB420
CsGy6G024310	Melon (DHL92) v3.6.1	cgybmedB443
CsGy6G024310	Watermelon (Charleston Gray)	cgybwcgB392
CsGy6G024310	Watermelon (Charleston Gray)	cgybwcgB406
CsGy6G024310	Watermelon (Charleston Gray)	cgybwcgB426
CsGy6G024310	Watermelon (97103) v1	cgybwmB423
CsGy6G024310	Watermelon (97103) v1	cgybwmB483
CsGy6G024310	Watermelon (97103) v1	cgybwmB472
CsGy6G024310	Wild cucumber (PI 183967)	cgybcpiB263
CsGy6G024310	Wild cucumber (PI 183967)	cgybcpiB287
CsGy6G024310	Wild cucumber (PI 183967)	cgybcpiB289
CsGy6G024310	Silver-seed gourd	carcgybB0255
CsGy6G024310	Silver-seed gourd	carcgybB0264
CsGy6G024310	Silver-seed gourd	carcgybB0654
CsGy6G024310	Cucumber (Chinese Long) v3	cgybcucB268
CsGy6G024310	Cucumber (Chinese Long) v3	cgybcucB298
CsGy6G024310	Cucumber (Chinese Long) v3	cgybcucB300
CsGy6G024310	Watermelon (97103) v2	cgybwmbB398
CsGy6G024310	Watermelon (97103) v2	cgybwmbB414
CsGy6G024310	Watermelon (97103) v2	cgybwmbB431
CsGy6G024310	Wax gourd	cgybwgoB504
CsGy6G024310	Wax gourd	cgybwgoB586
CsGy6G024310	Wax gourd	cgybwgoB599