CsGy5G010050 (gene) Cucumber (Gy14) v2

Overview
NameCsGy5G010050
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionPectinesterase
LocationChr5 : 8988508 .. 8989209 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCGTAGATCTGCAACCCTTGATAAGTATGTCGTTCAAAATTCTTTTATATATATTATGATCTTTTATTTCATATTATAGTTTTTTTCTTATATATATAATGTTTAACTAAACATTTTTATTTTATGTCGTGGTTAAAGGTAATAATTTTCTTGCACAATCATTGAGTTTTGAAAACTTCGTCGGTCCTCAAAATGGCCAAGCAGTGGCAGTTCTTGACCAAGCTCGCTTCGGTGCTTATCACAAGTGCAGATTCTTAGGTTTTCAAGACACTTTGTATGTTAAAGGCAAACCCCAATTCTTCAAAGAGTGTGATATCTTTGGCTCTCAACTTTATTTTTGGAAATGACTTAGTTATGTTTCAAGATTGTAACATATATGTCTGAGAAAAAAATAGATCTATCACAGTCACTGCTCTATCCAAAGAACAATTATAGGAAGAAAGCAGTTTCTCATTTCAAAATTAGACCATCATTGTTTCGTTGGAGATAACACAGCCAGCAAAGCTAACGTCAAAATCTATCTTGGCCGACTGTGGAGACAATATTCACGAGTAGTGTTCATGGAGTCATTTCTTAATGAGGAAGTGTTGCCTGAAGAATGGTCAGAGTGGCCAAGGGTACCAATGACCAATTTGTTTTACGAAGAGTTTAATAACCGTGGTTCTGGGGCAAATGTCTCTAAAATGGTACTTTGTTAA

mRNA sequence

ATGATCGTAGATCTGCAACCCTTGATAAGTAATAATTTTCTTGCACAATCATTGAGTTTTGAAAACTTCGTCGGTCCTCAAAATGGCCAAGCAGTGGCAGTTCTTGACCAAGCTCGCTTCGGTGCTTATCACAAGTGCAGATTCTTAGGTTTTCAAGACACTTTGTATGTTAAAGGCAAACCCCAATTCTTCAAAGATCACTGCTCTATCCAAAGAACAATTATAGGAAGAAAGCAGTTTCTCATTTCAAAATTAGACCATCATTGTTTCGTTGGAGATAACACAGCCAGCAAAGCTAACGTCAAAATCTATCTTGGCCGACTGTGGAGACAATATTCACGAGTAGTGTTCATGGAGTCATTTCTTAATGAGGAAGTGTTGCCTGAAGAATGGTCAGAGTGGCCAAGGGTACCAATGACCAATTTGTTTTACGAAGAGTTTAATAACCGTGGTTCTGGGGCAAATGTCTCTAAAATGGTACTTTGTTAA

Coding sequence (CDS)

ATGATCGTAGATCTGCAACCCTTGATAAGTAATAATTTTCTTGCACAATCATTGAGTTTTGAAAACTTCGTCGGTCCTCAAAATGGCCAAGCAGTGGCAGTTCTTGACCAAGCTCGCTTCGGTGCTTATCACAAGTGCAGATTCTTAGGTTTTCAAGACACTTTGTATGTTAAAGGCAAACCCCAATTCTTCAAAGATCACTGCTCTATCCAAAGAACAATTATAGGAAGAAAGCAGTTTCTCATTTCAAAATTAGACCATCATTGTTTCGTTGGAGATAACACAGCCAGCAAAGCTAACGTCAAAATCTATCTTGGCCGACTGTGGAGACAATATTCACGAGTAGTGTTCATGGAGTCATTTCTTAATGAGGAAGTGTTGCCTGAAGAATGGTCAGAGTGGCCAAGGGTACCAATGACCAATTTGTTTTACGAAGAGTTTAATAACCGTGGTTCTGGGGCAAATGTCTCTAAAATGGTACTTTGTTAA

Protein sequence

MIVDLQPLISNNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDHCSIQRTIIGRKQFLISKLDHHCFVGDNTASKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANVSKMVLC
Homology
[Close]
BLAST of CsGy5G010050 vs. NCBI nr
Match: XP_011656190.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 41 [Cucumis sativus])

HSP 1 Score: 183.7 bits (465), Expect = 4.9e-43
Identity = 98/185 (52.97%), Postives = 119/185 (64.32%), Query Frame = 0 

Query: 11  NNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKD---- 70
           NNFLAQSL+F+N  GPQNGQAVAVLD+A F  Y+KCRFLGFQDTLYV+GKPQFFK+    
Sbjct: 86  NNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIY 145

Query: 71  ---------------HCSIQRTIIGRKQFLI--SKLDHH---------CFV---GDNTAS 130
                           C+I      R   +   SK   H         C +    +  A+
Sbjct: 146 GSVDFIFGDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAAN 205

Query: 131 KANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANVS 163
           KA+VKIYLGR WRQYS+VVFMESFL++EV+P+ W +W  VP+ NLFY EFNNRG GA+VS
Sbjct: 206 KADVKIYLGRPWRQYSQVVFMESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVS 265

[Close]
BLAST of CsGy5G010050 vs. NCBI nr
Match: XP_008465596.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis melo])

HSP 1 Score: 167.5 bits (423), Expect = 3.6e-38
Identity = 89/183 (48.63%), Postives = 112/183 (61.20%), Query Frame = 0 

Query: 11  NNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKD---- 70
           NNF+A+SL+FEN  GPQNGQAVAV D+A   AY+KCRFLGFQDTLYV GK QFFK+    
Sbjct: 52  NNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIY 111

Query: 71  ---------------HCSIQR-------TIIGRKQFLISKLDHHCF-------VGDNTAS 130
                           C+I         T+  + +  +  +    F       + + +++
Sbjct: 112 GSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSN 171

Query: 131 KANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANVS 161
           K NVK++LGRLWRQYS VVFMESFL++ V  E W EW  VP+ NLFY EFNN G GA+VS
Sbjct: 172 KQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVS 231

[Close]
BLAST of CsGy5G010050 vs. NCBI nr
Match: XP_004152582.2 (PREDICTED: pectinesterase 2-like [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_1G049450 [Cucumis sativus])

HSP 1 Score: 163.7 bits (413), Expect = 5.2e-37
Identity = 85/183 (46.45%), Postives = 112/183 (61.20%), Query Frame = 0 

Query: 11  NNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKD---- 70
           +NF+A+SL+FEN  GPQN QAVAV D+A   AY+KCRFL FQDTLYV GKPQFFK+    
Sbjct: 113 DNFMAESLTFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIY 172

Query: 71  ---------------HCSIQR-------TIIGRKQFLISKLDHHCFVG-------DNTAS 130
                           C+I         T+  + ++++  +    F         + +++
Sbjct: 173 GSVDFICGYGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSN 232

Query: 131 KANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANVS 161
           K NVK++LGR W+QYS+VVFMESFL++ V  E W EW  VP+ NLFY EFNN G GA+VS
Sbjct: 233 KQNVKVFLGRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVS 292

[Close]
BLAST of CsGy5G010050 vs. NCBI nr
Match: XP_008465578.1 (PREDICTED: pectinesterase 2-like, partial [Cucumis melo])

HSP 1 Score: 162.2 bits (409), Expect = 1.5e-36
Identity = 87/183 (47.54%), Postives = 110/183 (60.11%), Query Frame = 0 

Query: 11  NNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKD---- 70
           +NF+A+SL+FEN  GPQNGQAVAV D+A   AY+KCRFLGFQDTLYV GK QFFK+    
Sbjct: 4   DNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIY 63

Query: 71  ---------------HCSIQR-------TIIGRKQFLISKLDHHCFVG-------DNTAS 130
                           C+I         T+  + +  +  +    F         + +++
Sbjct: 64  GSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSN 123

Query: 131 KANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANVS 161
           K NVK++LGR WRQYS VVFMESFL++ V  E W EW  VP+ NLFY EFNN G GA+VS
Sbjct: 124 KQNVKVFLGRPWRQYSNVVFMESFLDDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVS 183

[Close]
BLAST of CsGy5G010050 vs. NCBI nr
Match: XP_022952993.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata])

HSP 1 Score: 153.3 bits (386), Expect = 7.1e-34
Identity = 85/184 (46.20%), Postives = 106/184 (57.61%), Query Frame = 0 

Query: 11  NNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDHCSI 70
           +NF+AQ LSFEN   PQNGQAVAVLD A+   Y+ CRF+ FQDTLY KG+ QFF++ C I
Sbjct: 113 DNFMAQHLSFENSAEPQNGQAVAVLDYAKHSVYYDCRFMSFQDTLYSKGEFQFFRE-CDI 172

Query: 71  QRTI----------------------------IGRKQFLISKLD---HHCFV---GDNTA 130
             T+                               K+  +S+      +C V    + TA
Sbjct: 173 YGTVDFIFGNGLAVFQDCNIYARLPMDEITVTAQSKEQSVSQSGFSFQNCTVTVSPEITA 232

Query: 131 SKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANV 161
           +K  VKIYLGR WR+YS VVFMESFL++ V P+ W  W  VP+ NLFY EFNNRG GAN 
Sbjct: 233 NKTKVKIYLGRPWREYSTVVFMESFLDDNVQPQGWISWEGVPINNLFYAEFNNRGPGANT 292

[Close]
BLAST of CsGy5G010050 vs. TAIR10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 103.6 bits (257), Expect = 1.2e-22
Identity = 60/189 (31.75%), Postives = 91/189 (48.15%), Query Frame = 0 

Query: 10  SNNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDHCS 69
           S NF+A +++F N  GP+  QAVA+   A    ++ C F  +QDTLY     QF+++ C 
Sbjct: 346 SPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRE-CD 405

Query: 70  IQRT---IIGRKQFLISKLD------------------------------HHCFV---GD 129
           I  T   I G    +    +                              H+C +    D
Sbjct: 406 IYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADD 465

Query: 130 NTASKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEW-PRVPMTNLFYEEFNNRGS 162
             +S   VK YLGR W++YSR VFM+S+++E V P  W EW     ++ L+Y E+NN GS
Sbjct: 466 LVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGS 525

[Close]
BLAST of CsGy5G010050 vs. TAIR10
Match: AT3G14310.1 (pectin methylesterase 3)

HSP 1 Score: 98.2 bits (243), Expect = 4.9e-21
Identity = 59/189 (31.22%), Postives = 87/189 (46.03%), Query Frame = 0 

Query: 9   ISNNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDHC 68
           +   FLA+ ++F+N  GP   QAVA+   + F A++ C  L +QDTLYV    QFF   C
Sbjct: 365 VGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFV-KC 424

Query: 69  SIQRT---IIGRKQFLISKLDHH------------------------------CFVG--- 128
            I  T   I G    ++   D H                              C +G   
Sbjct: 425 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 484

Query: 129 DNTASKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWP-RVPMTNLFYEEFNNRG 161
           D  + K +   YLGR W++YS+ V M+S +++ + PE WSEW     +  L Y E++N G
Sbjct: 485 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 544

[Close]
BLAST of CsGy5G010050 vs. TAIR10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 97.1 bits (240), Expect = 1.1e-20
Identity = 56/186 (30.11%), Postives = 87/186 (46.77%), Query Frame = 0 

Query: 12  NFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDHCSIQ 71
           NF+A +++F N  GP+  QAVA+   A F  ++ C F  +QDTLY     QF+++ C + 
Sbjct: 354 NFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRE-CDVY 413

Query: 72  RT---IIGRKQFLISKLD------------------------------HHCFV---GDNT 131
            T   I G    +    +                               +C +    D  
Sbjct: 414 GTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV 473

Query: 132 ASKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEW-PRVPMTNLFYEEFNNRGSGA 161
           +S   VK YLGR W++YSR V+M+S+++  V P  W EW     ++ L+Y E+NN G G+
Sbjct: 474 SSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGS 533

[Close]
BLAST of CsGy5G010050 vs. TAIR10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 96.3 bits (238), Expect = 1.9e-20
Identity = 57/178 (32.02%), Postives = 87/178 (48.88%), Query Frame = 0 

Query: 11  NNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDHCSI 70
           N F+AQ ++FEN  GP+  QAVA+   + F  ++ C F G+QDTLY+    QF + +C+I
Sbjct: 338 NGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLR-NCNI 397

Query: 71  QRTI----------------------IGRKQFLISKL-----DHHCFVGDNTASKANVKI 130
             T+                       G+K  + ++      +   FV  ++      + 
Sbjct: 398 YGTVDFIFGDATAIXXXXXXXARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASET 457

Query: 131 YLGRLWRQYSRVVFMESFLNEEVLPEEWSEWP-RVPMTNLFYEEFNNRGSGANVSKMV 161
           YLGR WR +SR VFM+  L   V P  W  W     ++ L+Y E+ N G+GA+VS  V
Sbjct: 458 YLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASVSGRV 514

[Close]
BLAST of CsGy5G010050 vs. TAIR10
Match: AT1G53830.1 (pectin methylesterase 2)

HSP 1 Score: 96.3 bits (238), Expect = 1.9e-20
Identity = 60/188 (31.91%), Postives = 89/188 (47.34%), Query Frame = 0 

Query: 9   ISNNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFF-KDH 68
           +   FLA+ ++F+N  GP   QAVA+   + F A+++C    +QDTLYV    QFF K H
Sbjct: 360 VGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCH 419

Query: 69  ------------------CSI--QRTIIGRKQFLISK-----------LDHHCFVG---D 128
                             C I  +R   G+K  + ++           +  +C +G   D
Sbjct: 420 ITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSD 479

Query: 129 NTASKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWP-RVPMTNLFYEEFNNRGS 161
             A K     YLGR W++YSR V M+S +++ + PE W EW     +  L Y E+ NRG 
Sbjct: 480 LLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGG 539

[Close]
BLAST of CsGy5G010050 vs. Swiss-Prot
Match: sp|Q8RXK7|PME41_ARATH (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 103.6 bits (257), Expect = 2.1e-21
Identity = 60/189 (31.75%), Postives = 91/189 (48.15%), Query Frame = 0 

Query: 10  SNNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDHCS 69
           S NF+A +++F N  GP+  QAVA+   A    ++ C F  +QDTLY     QF+++ C 
Sbjct: 346 SPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRE-CD 405

Query: 70  IQRT---IIGRKQFLISKLD------------------------------HHCFV---GD 129
           I  T   I G    +    +                              H+C +    D
Sbjct: 406 IYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADD 465

Query: 130 NTASKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEW-PRVPMTNLFYEEFNNRGS 162
             +S   VK YLGR W++YSR VFM+S+++E V P  W EW     ++ L+Y E+NN GS
Sbjct: 466 LVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGS 525

[Close]
BLAST of CsGy5G010050 vs. Swiss-Prot
Match: sp|P85076|PME_ACTDE (Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.8e-20
Identity = 59/190 (31.05%), Postives = 90/190 (47.37%), Query Frame = 0 

Query: 8   LISNNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDH 67
           ++   F+A+ +SFEN+ GP   QAVA+   A F A+++C F+G+QDTLYV    QF+ + 
Sbjct: 92  VVGTGFIARGISFENYAGPSKHQAVALRSGADFSAFYQCSFVGYQDTLYVHSLRQFYSE- 151

Query: 68  CSIQRT---IIGRKQFLISKLDHH------------------------------CFV--- 127
           C +  T   I G    ++ K + +                              C V   
Sbjct: 152 CDVYGTIDFIFGNAAAVLQKCNLYARKPNENQKNIFTAQGRDDPNQNTGISILNCKVAAA 211

Query: 128 GDNTASKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWP-RVPMTNLFYEEFNNR 161
            D     ++ K YLGR W++YSR VF+ S +   + P  W EW     +T L+Y E+ N 
Sbjct: 212 ADLIPVLSSFKTYLGRPWKEYSRTVFLLSQMESLIDPAGWLEWSGDFALTTLYYREYKNT 271

[Close]
BLAST of CsGy5G010050 vs. Swiss-Prot
Match: sp|O04887|PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.0e-20
Identity = 55/189 (29.10%), Postives = 93/189 (49.21%), Query Frame = 0 

Query: 8   LISNNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDH 67
           ++ +NF+A+ ++  N  GP N QAVA+   +    +++C F G+QDTLYV  + QF+++ 
Sbjct: 283 VVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRE- 342

Query: 68  CSIQRT---IIGRKQFLISKLD-----------------------------HHCFV---G 127
           C I  T   I G    ++   +                             H+C V    
Sbjct: 343 CDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQGRTDPNQSTGIIIHNCRVTAAS 402

Query: 128 DNTASKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWP-RVPMTNLFYEEFNNRG 161
           D    +++VK +LGR W+QYSR V++++FL+  + P  W EW     +  L+Y E+ N G
Sbjct: 403 DLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTG 462

[Close]
BLAST of CsGy5G010050 vs. Swiss-Prot
Match: sp|O49006|PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 98.2 bits (243), Expect = 8.8e-20
Identity = 59/189 (31.22%), Postives = 87/189 (46.03%), Query Frame = 0 

Query: 9   ISNNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDHC 68
           +   FLA+ ++F+N  GP   QAVA+   + F A++ C  L +QDTLYV    QFF   C
Sbjct: 365 VGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFV-KC 424

Query: 69  SIQRT---IIGRKQFLISKLDHH------------------------------CFVG--- 128
            I  T   I G    ++   D H                              C +G   
Sbjct: 425 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 484

Query: 129 DNTASKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWP-RVPMTNLFYEEFNNRG 161
           D  + K +   YLGR W++YS+ V M+S +++ + PE WSEW     +  L Y E++N G
Sbjct: 485 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 544

[Close]
BLAST of CsGy5G010050 vs. Swiss-Prot
Match: sp|Q9SRX4|PME7_ARATH (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.0e-19
Identity = 56/186 (30.11%), Postives = 87/186 (46.77%), Query Frame = 0 

Query: 12  NFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKDHCSIQ 71
           NF+A +++F N  GP+  QAVA+   A F  ++ C F  +QDTLY     QF+++ C + 
Sbjct: 354 NFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRE-CDVY 413

Query: 72  RT---IIGRKQFLISKLD------------------------------HHCFV---GDNT 131
            T   I G    +    +                               +C +    D  
Sbjct: 414 GTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV 473

Query: 132 ASKANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEW-PRVPMTNLFYEEFNNRGSGA 161
           +S   VK YLGR W++YSR V+M+S+++  V P  W EW     ++ L+Y E+NN G G+
Sbjct: 474 SSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGS 533

[Close]
BLAST of CsGy5G010050 vs. TrEMBL
Match: tr|A0A1S3CP85|A0A1S3CP85_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503231 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.4e-38
Identity = 89/183 (48.63%), Postives = 112/183 (61.20%), Query Frame = 0 

Query: 11  NNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKD---- 70
           NNF+A+SL+FEN  GPQNGQAVAV D+A   AY+KCRFLGFQDTLYV GK QFFK+    
Sbjct: 52  NNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIY 111

Query: 71  ---------------HCSIQR-------TIIGRKQFLISKLDHHCF-------VGDNTAS 130
                           C+I         T+  + +  +  +    F       + + +++
Sbjct: 112 GSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSN 171

Query: 131 KANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANVS 161
           K NVK++LGRLWRQYS VVFMESFL++ V  E W EW  VP+ NLFY EFNN G GA+VS
Sbjct: 172 KQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVS 231

[Close]
BLAST of CsGy5G010050 vs. TrEMBL
Match: tr|A0A0A0LR14|A0A0A0LR14_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.5e-37
Identity = 85/183 (46.45%), Postives = 112/183 (61.20%), Query Frame = 0 

Query: 11  NNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKD---- 70
           +NF+A+SL+FEN  GPQN QAVAV D+A   AY+KCRFL FQDTLYV GKPQFFK+    
Sbjct: 113 DNFMAESLTFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIY 172

Query: 71  ---------------HCSIQR-------TIIGRKQFLISKLDHHCFVG-------DNTAS 130
                           C+I         T+  + ++++  +    F         + +++
Sbjct: 173 GSVDFICGYGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSN 232

Query: 131 KANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANVS 161
           K NVK++LGR W+QYS+VVFMESFL++ V  E W EW  VP+ NLFY EFNN G GA+VS
Sbjct: 233 KQNVKVFLGRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVS 292

[Close]
BLAST of CsGy5G010050 vs. TrEMBL
Match: tr|A0A1S3CP42|A0A1S3CP42_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503212 PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.0e-36
Identity = 87/183 (47.54%), Postives = 110/183 (60.11%), Query Frame = 0 

Query: 11  NNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKD---- 70
           +NF+A+SL+FEN  GPQNGQAVAV D+A   AY+KCRFLGFQDTLYV GK QFFK+    
Sbjct: 4   DNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIY 63

Query: 71  ---------------HCSIQR-------TIIGRKQFLISKLDHHCFVG-------DNTAS 130
                           C+I         T+  + +  +  +    F         + +++
Sbjct: 64  GSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSN 123

Query: 131 KANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANVS 161
           K NVK++LGR WRQYS VVFMESFL++ V  E W EW  VP+ NLFY EFNN G GA+VS
Sbjct: 124 KQNVKVFLGRPWRQYSNVVFMESFLDDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVS 183

[Close]
BLAST of CsGy5G010050 vs. TrEMBL
Match: tr|A0A1S3CQ52|A0A1S3CQ52_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503419 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.1e-30
Identity = 76/182 (41.76%), Postives = 100/182 (54.95%), Query Frame = 0 

Query: 12  NFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKD----- 71
           NF+AQ L+F+N  GP  GQA+AVLDQA+  AY++C FLG+QDTLY    PQFFK+     
Sbjct: 103 NFMAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYRCVFLGYQDTLYAGSVPQFFKECDIYG 162

Query: 72  --------------HCSI-------QRTIIGRKQFLISKLDHHCFVGDN-------TASK 131
                          C+I       Q T+  + +  +S      F   N        +SK
Sbjct: 163 SVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQSLSAPSGFIFQNCNVTVSPEIASSK 222

Query: 132 ANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANVSK 161
             V ++LGR WR YS VVF++SFL+  V P+ W EWP VP   L+Y E+NN G GAN S+
Sbjct: 223 DKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEWPGVPENVLYYAEYNNSGGGANTSQ 282

[Close]
BLAST of CsGy5G010050 vs. TrEMBL
Match: tr|A0A0A0L1M3|A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 7.0e-30
Identity = 75/183 (40.98%), Postives = 101/183 (55.19%), Query Frame = 0 

Query: 11  NNFLAQSLSFENFVGPQNGQAVAVLDQARFGAYHKCRFLGFQDTLYVKGKPQFFKD---- 70
           +NF+AQ L+F+N  GP  GQA+A+LDQA   AY+KC FLG+QDTLY    PQFFK+    
Sbjct: 108 SNFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIY 167

Query: 71  ---------------HCSI-------QRTIIGRKQFLISKLDHHCFVG-------DNTAS 130
                           C+I       Q T+  + +  ++ L    F         +  +S
Sbjct: 168 GSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASS 227

Query: 131 KANVKIYLGRLWRQYSRVVFMESFLNEEVLPEEWSEWPRVPMTNLFYEEFNNRGSGANVS 161
           K  V ++LGR WR YS VVF++SFL+  V P+ W EWP VP   L+Y E+NN G GAN S
Sbjct: 228 KDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTS 287

The following BLAST results are available for this feature:
BLAST of CsGy5G010050 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011656190.14.9e-4352.97PREDICTED: probable pectinesterase/pectinesterase inhibitor 41 [Cucumis sativus][more]
XP_008465596.13.6e-3848.63PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis... [more]
XP_004152582.25.2e-3746.45PREDICTED: pectinesterase 2-like [Cucumis sativus] >KGN64355.1 hypothetical prot... [more]
XP_008465578.11.5e-3647.54PREDICTED: pectinesterase 2-like, partial [Cucumis melo][more]
XP_022952993.17.1e-3446.20probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata][more]
BLAST of CsGy5G010050 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G02330.11.2e-2231.75Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G14310.14.9e-2131.22pectin methylesterase 3[more]
AT1G02810.11.1e-2030.11Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G23200.11.9e-2032.02Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G53830.11.9e-2031.91pectin methylesterase 2[more]
BLAST of CsGy5G010050 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q8RXK7|PME41_ARATH2.1e-2131.75Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
sp|P85076|PME_ACTDE1.8e-2031.05Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
sp|O04887|PME2_CITSI4.0e-2029.10Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
sp|O49006|PME3_ARATH8.8e-2031.22Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
sp|Q9SRX4|PME7_ARATH2.0e-1930.11Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
BLAST of CsGy5G010050 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3CP85|A0A1S3CP85_CUCME2.4e-3848.63Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503231 PE=4 SV=1[more]
tr|A0A0A0LR14|A0A0A0LR14_CUCSA3.5e-3746.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1[more]
tr|A0A1S3CP42|A0A1S3CP42_CUCME1.0e-3647.54Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503212 PE=4 SV=1[more]
tr|A0A1S3CQ52|A0A1S3CQ52_CUCME3.1e-3041.76Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503419 PE=4 SV=1[more]
tr|A0A0A0L1M3|A0A0A0L1M3_CUCSA7.0e-3040.98Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: INTERPRO
TermDefinition
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
IPR012334Pectin_lyas_fold
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0042545 cell wall modification
cellular_component GO:0005575 cellular_component
cellular_component GO:0005618 cell wall
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G010050.1CsGy5G010050.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 82..161
e-value: 5.7E-17
score: 64.0
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 7..81
e-value: 1.2E-14
score: 56.3
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 93..160
e-value: 3.0E-17
score: 62.2
coord: 10..74
e-value: 3.5E-16
score: 58.7
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 95..159
coord: 11..74
NoneNo IPR availablePANTHERPTHR31707:SF126PECTINESTERASE 30-RELATEDcoord: 95..159
coord: 11..74
IPR011050Pectin lyase fold/virulence factorSUPERFAMILYSSF51126Pectin lyase-likecoord: 9..159

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsGy5G010050Cucurbita maxima (Rimu)cgybcmaB664
CsGy5G010050Cucurbita moschata (Rifu)cgybcmoB641
CsGy5G010050Cucurbita pepo (Zucchini)cgybcpeB642
CsGy5G010050Cucumber (Chinese Long) v2cgybcuB232
CsGy5G010050Bottle gourd (USVL1VR-Ls)cgyblsiB307
CsGy5G010050Melon (DHL92) v3.5.1cgybmeB328
CsGy5G010050Melon (DHL92) v3.6.1cgybmedB313
CsGy5G010050Watermelon (Charleston Gray)cgybwcgB329
CsGy5G010050Watermelon (97103) v1cgybwmB410
CsGy5G010050Wild cucumber (PI 183967)cgybcpiB235
CsGy5G010050Silver-seed gourdcarcgybB0191
CsGy5G010050Cucumber (Chinese Long) v3cgybcucB232
CsGy5G010050Watermelon (97103) v2cgybwmbB342
CsGy5G010050Wax gourdcgybwgoB491