CsGy5G007360 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy5G007360
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	subtilase 4.12
Location	Chr5 : 5102475 .. 5103300 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAGCAAATTAGAAGATACGAGTTCTACTCCTTTGCATCATAGGGCAATGTTGGAACAAGTTGTTGGGAGGTTTGTATTTTATATATTTATTTATCTATATATTAAGCAAAGAAAAATAAGAAACTATAAAATTTTTGTTATGTTTGTAATTATTTATTGGGACTGTATGATATCCATAAAATGTCAAAAGTTTTTAATACTTTATTGCTTATCTTCTAGCAATTTTGCGCCGAAACACTTGCTGTATAGCTACAAGAGAAGTTTCAATGGATTTGCAGTGAGATTAACTGAAGAAGAAGCTCAAAAGATTGCTTGTATGTATATATTGTTCTAATAATACTTTTTCTTTTTTATATATTTTTAGGTAGTACAAGTTTGTTCAAAGTTGATCACTTTTAATTTATCACATTGCTTTTAATTAATAACAATTTTGGCTATAGGAAAATTTTGGGAAGAAATGTAAATTAATCTGAACTTGTTTAATTAAATTGATTTTAAATATGAGAACAGTGATAATCTCCTTTGAAATTATTTTGTTAACTAAATGTGAAAGATTAATAATATTTTCAGTGAAGGAGGGTGTGGTGTCGGTGTTTCCAAATGGAAAGAAGCATGTTCATACAACAAGGTCTTGGGATTTCATGGGTTTTACACAAAGTGTTCCTCGTGTAAACCAAGTTGAAAGCAACATAGTTGTTGGGGTTCTAGATACCGGAATTTGGCCGGAATCTCCTAGTTTCAATGACACAGATCTCGGACCTCCACCGGCCGGTTGGAAGGGTCAATGCCAAACATCCCCCGACTTTCAATGCAACAGGTGA

mRNA sequence

ATGGGAAGCAAATTAGAAGATACGAGTTCTACTCCTTTGCATCATAGGGCAATGTTGGAACAAGTTGTTGGGAGCAATTTTGCGCCGAAACACTTGCTGTATAGCTACAAGAGAAGTTTCAATGGATTTGCAGTGAGATTAACTGAAGAAGAAGCTCAAAAGATTGCTTTGAAGGAGGGTGTGGTGTCGGTGTTTCCAAATGGAAAGAAGCATGTTCATACAACAAGGTCTTGGGATTTCATGGGTTTTACACAAAGTGTTCCTCGTGTAAACCAAGTTGAAAGCAACATAGTTGTTGGGGTTCTAGATACCGGAATTTGGCCGGAATCTCCTAGTTTCAATGACACAGATCTCGGACCTCCACCGGCCGGTTGGAAGGGTCAATGCCAAACATCCCCCGACTTTCAATGCAACAGGTGA

Coding sequence (CDS)

ATGGGAAGCAAATTAGAAGATACGAGTTCTACTCCTTTGCATCATAGGGCAATGTTGGAACAAGTTGTTGGGAGCAATTTTGCGCCGAAACACTTGCTGTATAGCTACAAGAGAAGTTTCAATGGATTTGCAGTGAGATTAACTGAAGAAGAAGCTCAAAAGATTGCTTTGAAGGAGGGTGTGGTGTCGGTGTTTCCAAATGGAAAGAAGCATGTTCATACAACAAGGTCTTGGGATTTCATGGGTTTTACACAAAGTGTTCCTCGTGTAAACCAAGTTGAAAGCAACATAGTTGTTGGGGTTCTAGATACCGGAATTTGGCCGGAATCTCCTAGTTTCAATGACACAGATCTCGGACCTCCACCGGCCGGTTGGAAGGGTCAATGCCAAACATCCCCCGACTTTCAATGCAACAGGTGA

Protein sequence

MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGPPPAGWKGQCQTSPDFQCNR

Homology

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BLAST of CsGy5G007360 vs. NCBI nr
Match: XP_004149954.1 (PREDICTED: cucumisin-like [Cucumis sativus])

HSP 1 Score: 294.7 bits (753), Expect = 1.7e-76
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG
Sbjct: 42  MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 101

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP
Sbjct: 102 VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 161

Query: 121 PPAGWKGQCQTSPDFQCNR 140
           PPAGWKGQCQTSPDFQCNR
Sbjct: 162 PPAGWKGQCQTSPDFQCNR 180

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BLAST of CsGy5G007360 vs. NCBI nr
Match: KGN50585.1 (hypothetical protein Csa_5G187340 [Cucumis sativus])

HSP 1 Score: 294.7 bits (753), Expect = 1.7e-76
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG
Sbjct: 42  MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 101

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP
Sbjct: 102 VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 161

Query: 121 PPAGWKGQCQTSPDFQCNR 140
           PPAGWKGQCQTSPDFQCNR
Sbjct: 162 PPAGWKGQCQTSPDFQCNR 180

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BLAST of CsGy5G007360 vs. NCBI nr
Match: XP_008461717.1 (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 287.3 bits (734), Expect = 2.7e-74
Identity = 135/139 (97.12%), Postives = 136/139 (97.84%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MGS LEDTSSTPLHHRAMLEQV+GSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG
Sbjct: 44  MGSTLEDTSSTPLHHRAMLEQVIGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 103

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFND DL P
Sbjct: 104 VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDADLDP 163

Query: 121 PPAGWKGQCQTSPDFQCNR 140
           PPAGWKGQCQTSPDFQCNR
Sbjct: 164 PPAGWKGQCQTSPDFQCNR 182

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BLAST of CsGy5G007360 vs. NCBI nr
Match: XP_023548142.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.0 bits (650), Expect = 1.5e-64
Identity = 121/139 (87.05%), Postives = 127/139 (91.37%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MGSK ED SST L+HR MLEQVVGS+F+PKHLLYSYKRSFNGFAVRLT+EEAQKIAL+EG
Sbjct: 42  MGSKPEDISSTSLYHRTMLEQVVGSSFSPKHLLYSYKRSFNGFAVRLTKEEAQKIALEEG 101

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFPN KKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFND  + P
Sbjct: 102 VVSVFPNEKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDEGVDP 161

Query: 121 PPAGWKGQCQTSPDFQCNR 140
           PP  WKG CQ SPDFQCNR
Sbjct: 162 PPKEWKGTCQISPDFQCNR 180

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BLAST of CsGy5G007360 vs. NCBI nr
Match: XP_022139190.1 (LOW QUALITY PROTEIN: cucumisin-like [Momordica charantia])

HSP 1 Score: 236.1 bits (601), Expect = 7.1e-59
Identity = 111/142 (78.17%), Postives = 124/142 (87.32%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNF---APKHLLYSYKRSFNGFAVRLTEEEAQKIAL 60
           MGSK EDT+STPLHHRAMLE+V+GS F   AP+ LLYSYKRSFNGFAVRLTEEEA K+A 
Sbjct: 42  MGSKPEDTTSTPLHHRAMLEEVIGSTFFLAAPESLLYSYKRSFNGFAVRLTEEEALKVAA 101

Query: 61  KEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           KEGVVSVFPNGKKH+HTTRSWDFMGF+QSVPR +QVES++VVGVLDTGIWPESPSF D  
Sbjct: 102 KEGVVSVFPNGKKHLHTTRSWDFMGFSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEA 161

Query: 121 LGPPPAGWKGQCQTSPDFQCNR 140
            GPPPA W G CQ + DF+CN+
Sbjct: 162 FGPPPAKWMGACQAADDFRCNK 183

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BLAST of CsGy5G007360 vs. TAIR10
Match: AT5G59190.1 (subtilase family protein)

HSP 1 Score: 143.7 bits (361), Expect = 8.7e-35
Identity = 68/138 (49.28%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MG+  E   S P HH ++L+++VG+  A   L+ SYKRSFNGFA  L++ E+QK+   + 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFP+    + TTRSWDF+GF +   R +  ES+++VGV+D+GIWPES SF+D   GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 121 PPAGWKGQCQTSPDFQCN 139
           PP  WKG C+    F CN
Sbjct: 121 PPKKWKGSCKGGLKFACN 138

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BLAST of CsGy5G007360 vs. TAIR10
Match: AT5G59090.1 (subtilase 4.12)

HSP 1 Score: 139.8 bits (351), Expect = 1.3e-33
Identity = 68/126 (53.97%), Postives = 83/126 (65.87%), Query Frame = 0


Query: 15  HRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHVHT 74
           H ++L+QV G +     L+ SYKRSFNGFA RLTE E   IA  EGVVSVFPN    +HT
Sbjct: 52  HMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHT 111

Query: 75  TRSWDFMGFTQ--SVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGPPPAGWKGQCQTS 134
           T SWDFMG  +  +  R   +ES+ ++GV+DTGIWPES SF+D   GPPP  WKG C   
Sbjct: 112 TTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGG 171

Query: 135 PDFQCN 139
            +F CN
Sbjct: 172 KNFTCN 177

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BLAST of CsGy5G007360 vs. TAIR10
Match: AT4G15040.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 138.7 bits (348), Expect = 2.8e-33
Identity = 68/140 (48.57%), Postives = 92/140 (65.71%), Query Frame = 0


Query: 1   MGSKLEDTSSTPL-HHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKE 60
           MG+     S +P+ HH+ +L++V+ S+    +L+ SY RSFNGFA +LTE E  K+   E
Sbjct: 1   MGALPSKISYSPMSHHQNILQEVIESSSVEDYLVRSYGRSFNGFAAKLTESEKDKLIGME 60

Query: 61  GVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLG 120
           GVVSVFP+    + TTRS++FMG       V +VESN++VGV+D GIWPES SF+D  +G
Sbjct: 61  GVVSVFPSTVYKLFTTRSYEFMGLGDKSNNVPEVESNVIVGVIDGGIWPESKSFSDEGIG 120

Query: 121 PPPAGWKGQCQTSPDFQCNR 140
           P P  WKG C    +F CNR
Sbjct: 121 PIPKKWKGTCAGGTNFTCNR 140

[Close]

BLAST of CsGy5G007360 vs. TAIR10
Match: AT5G58820.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 138.7 bits (348), Expect = 2.8e-33
Identity = 69/141 (48.94%), Postives = 89/141 (63.12%), Query Frame = 0


Query: 1   MGSKLEDTSSTPL-HHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKE 60
           MGS       TPL HH ++L++V G +     L+ SYKRSFNGFA RLTE E  ++A  E
Sbjct: 34  MGSLPSLLEYTPLSHHMSILQEVTGDSSVEGRLVRSYKRSFNGFAARLTESERIRVAEME 93

Query: 61  GVVSVFPNGKKHVHTTRSWDFMGFTQ--SVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           GVVSVFPN    + TT SWDF+G  +  +  R   +ES+ ++G +D+GIWPES SF+D  
Sbjct: 94  GVVSVFPNINYKLQTTASWDFLGLKEGKNTKRNLAIESDTIIGFIDSGIWPESESFSDKG 153

Query: 121 LGPPPAGWKGQCQTSPDFQCN 139
            GPPP  WKG C    +F CN
Sbjct: 154 FGPPPKKWKGVCSGGKNFTCN 174

[Close]

BLAST of CsGy5G007360 vs. TAIR10
Match: AT5G58840.1 (Subtilase family protein)

HSP 1 Score: 137.5 bits (345), Expect = 6.3e-33
Identity = 66/138 (47.83%), Postives = 90/138 (65.22%), Query Frame = 0


Query: 3   SKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVV 62
           S+LE T  +  HH ++L++V G +     L+ SYKRSFNGFA RLTE E +++A  EGVV
Sbjct: 45  SRLEYTPMS--HHMSILQEVTGESSVEGRLVRSYKRSFNGFAARLTESERERVAEMEGVV 104

Query: 63  SVFPNGKKHVHTTRSWDFMGFTQ--SVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 122
           SVFP+    + TT SWDF+G  +  +  R   +ES+ ++G +D+GIWPES SF+D   GP
Sbjct: 105 SVFPDINYKLQTTASWDFLGLKEGKNTKRNLAIESDTIIGFIDSGIWPESESFSDKGFGP 164

Query: 123 PPAGWKGQCQTSPDFQCN 139
           PP  WKG C    +F CN
Sbjct: 165 PPKKWKGVCSAGKNFTCN 180

[Close]

BLAST of CsGy5G007360 vs. Swiss-Prot
Match: sp|Q39547|CUCM1_CUCME (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 6.1e-54
Identity = 100/139 (71.94%), Postives = 114/139 (82.01%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MG KLED  S  LHHRAMLEQVVGS FAP+ +L++YKRSFNGFAV+LTEEEA+KIA  EG
Sbjct: 38  MGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEG 97

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVF N    +HTTRSWDF+GF  +VPR +QVESNIVVGVLDTGIWPESPSF+D    P
Sbjct: 98  VVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSP 157

Query: 121 PPAGWKGQCQTSPDFQCNR 140
           PP  WKG C+TS +F+CNR
Sbjct: 158 PPPKWKGTCETSNNFRCNR 176

[Close]

BLAST of CsGy5G007360 vs. Swiss-Prot
Match: sp|Q9FIF8|SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 1.6e-33
Identity = 68/138 (49.28%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MG+  E   S P HH ++L+++VG+  A   L+ SYKRSFNGFA  L++ E+QK+   + 
Sbjct: 37  MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 96

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFP+    + TTRSWDF+GF +   R +  ES+++VGV+D+GIWPES SF+D   GP
Sbjct: 97  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 156

Query: 121 PPAGWKGQCQTSPDFQCN 139
           PP  WKG C+    F CN
Sbjct: 157 PPKKWKGSCKGGLKFACN 174

[Close]

BLAST of CsGy5G007360 vs. Swiss-Prot
Match: sp|Q8L7D2|SBT4C_ARATH (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.3e-32
Identity = 68/126 (53.97%), Postives = 83/126 (65.87%), Query Frame = 0


Query: 15  HRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHVHT 74
           H ++L+QV G +     L+ SYKRSFNGFA RLTE E   IA  EGVVSVFPN    +HT
Sbjct: 52  HMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHT 111

Query: 75  TRSWDFMGFTQ--SVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGPPPAGWKGQCQTS 134
           T SWDFMG  +  +  R   +ES+ ++GV+DTGIWPES SF+D   GPPP  WKG C   
Sbjct: 112 TTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGG 171

Query: 135 PDFQCN 139
            +F CN
Sbjct: 172 KNFTCN 177

[Close]

BLAST of CsGy5G007360 vs. Swiss-Prot
Match: sp|O23357|SBT42_ARATH (Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana OX=3702 GN=SBT4.2 PE=3 SV=3)

HSP 1 Score: 138.7 bits (348), Expect = 5.1e-32
Identity = 68/140 (48.57%), Postives = 92/140 (65.71%), Query Frame = 0


Query: 1   MGSKLEDTSSTPL-HHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKE 60
           MG+     S +P+ HH+ +L++V+ S+    +L+ SY RSFNGFA +LTE E  K+   E
Sbjct: 39  MGALPSKISYSPMSHHQNILQEVIESSSVEDYLVRSYGRSFNGFAAKLTESEKDKLIGME 98

Query: 61  GVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLG 120
           GVVSVFP+    + TTRS++FMG       V +VESN++VGV+D GIWPES SF+D  +G
Sbjct: 99  GVVSVFPSTVYKLFTTRSYEFMGLGDKSNNVPEVESNVIVGVIDGGIWPESKSFSDEGIG 158

Query: 121 PPPAGWKGQCQTSPDFQCNR 140
           P P  WKG C    +F CNR
Sbjct: 159 PIPKKWKGTCAGGTNFTCNR 178

[Close]

BLAST of CsGy5G007360 vs. Swiss-Prot
Match: sp|F4KGD4|SBT47_ARATH (Subtilisin-like protease SBT4.7 OS=Arabidopsis thaliana OX=3702 GN=SBT4.7 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 5.1e-32
Identity = 69/141 (48.94%), Postives = 89/141 (63.12%), Query Frame = 0


Query: 1   MGSKLEDTSSTPL-HHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKE 60
           MGS       TPL HH ++L++V G +     L+ SYKRSFNGFA RLTE E  ++A  E
Sbjct: 34  MGSLPSLLEYTPLSHHMSILQEVTGDSSVEGRLVRSYKRSFNGFAARLTESERIRVAEME 93

Query: 61  GVVSVFPNGKKHVHTTRSWDFMGFTQ--SVPRVNQVESNIVVGVLDTGIWPESPSFNDTD 120
           GVVSVFPN    + TT SWDF+G  +  +  R   +ES+ ++G +D+GIWPES SF+D  
Sbjct: 94  GVVSVFPNINYKLQTTASWDFLGLKEGKNTKRNLAIESDTIIGFIDSGIWPESESFSDKG 153

Query: 121 LGPPPAGWKGQCQTSPDFQCN 139
            GPPP  WKG C    +F CN
Sbjct: 154 FGPPPKKWKGVCSGGKNFTCN 174

[Close]

BLAST of CsGy5G007360 vs. TrEMBL
Match: tr|A0A0A0KLR1|A0A0A0KLR1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187340 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.1e-76
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG
Sbjct: 42  MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 101

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP
Sbjct: 102 VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 161

Query: 121 PPAGWKGQCQTSPDFQCNR 140
           PPAGWKGQCQTSPDFQCNR
Sbjct: 162 PPAGWKGQCQTSPDFQCNR 180

[Close]

BLAST of CsGy5G007360 vs. TrEMBL
Match: tr|A0A1S3CF95|A0A1S3CF95_CUCME (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.8e-74
Identity = 135/139 (97.12%), Postives = 136/139 (97.84%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MGS LEDTSSTPLHHRAMLEQV+GSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG
Sbjct: 44  MGSTLEDTSSTPLHHRAMLEQVIGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 103

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFND DL P
Sbjct: 104 VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDADLDP 163

Query: 121 PPAGWKGQCQTSPDFQCNR 140
           PPAGWKGQCQTSPDFQCNR
Sbjct: 164 PPAGWKGQCQTSPDFQCNR 182

[Close]

BLAST of CsGy5G007360 vs. TrEMBL
Match: tr|A0A1S3CFE1|A0A1S3CFE1_CUCME (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500264 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.1e-58
Identity = 108/139 (77.70%), Postives = 123/139 (88.49%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MG+K ED++STP HH  MLE+VVGS+FAP+ LL+SYKRSFNGF V+LTEEEAQKI+ KE 
Sbjct: 51  MGNKPEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 110

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFPN KKH+HTTRSWDFMGFTQ+VPRV QVESNIVVGVLD+GIWPESPSF+D   GP
Sbjct: 111 VVSVFPNEKKHLHTTRSWDFMGFTQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 170

Query: 121 PPAGWKGQCQTSPDFQCNR 140
           PPA WKG CQTS +F+CNR
Sbjct: 171 PPAKWKGACQTSANFRCNR 189

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BLAST of CsGy5G007360 vs. TrEMBL
Match: tr|A0A0A0KLR4|A0A0A0KLR4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187880 PE=4 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 8.9e-58
Identity = 107/139 (76.98%), Postives = 120/139 (86.33%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MG+KLED++STP HH  MLE+VVGS+FAP+ LL+SYKRSFNGF V+LTEEEAQKI+ KE 
Sbjct: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFPN KKH+HTTRSWDFMGFTQ  PRV QVESNIVVGVLD+GIWPESPSF+D   GP
Sbjct: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120

Query: 121 PPAGWKGQCQTSPDFQCNR 140
           PP  WKG CQTS +F CNR
Sbjct: 121 PPPKWKGACQTSANFHCNR 139

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BLAST of CsGy5G007360 vs. TrEMBL
Match: tr|A0A1S3CF99|A0A1S3CF99_CUCME (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500258 PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.4e-55
Identity = 101/139 (72.66%), Postives = 120/139 (86.33%), Query Frame = 0


Query: 1   MGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 60
           MG+K +DT+STP HH  ML +V GSNFAP+ LL+SYKRSFNGF V+LTEEEA +I+ KEG
Sbjct: 53  MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG 112

Query: 61  VVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDTDLGP 120
           VVSVFP+GKKH+HTTRSWDF+GFT+ VPRVNQVES+IVVGVLD+GIWPE+PSF+D   GP
Sbjct: 113 VVSVFPSGKKHLHTTRSWDFLGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDVGYGP 172

Query: 121 PPAGWKGQCQTSPDFQCNR 140
            PA WKG CQTS +F CN+
Sbjct: 173 IPAKWKGSCQTSANFTCNK 191

The following BLAST results are available for this feature:

BLAST of CsGy5G007360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149954.1	1.7e-76	100.00	PREDICTED: cucumisin-like [Cucumis sativus]	[more]
KGN50585.1	1.7e-76	100.00	hypothetical protein Csa_5G187340 [Cucumis sativus]	[more]
XP_008461717.1	2.7e-74	97.12	PREDICTED: cucumisin-like [Cucumis melo]	[more]
XP_023548142.1	1.5e-64	87.05	cucumisin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022139190.1	7.1e-59	78.17	LOW QUALITY PROTEIN: cucumisin-like [Momordica charantia]	[more]

BLAST of CsGy5G007360 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G59190.1	8.7e-35	49.28	subtilase family protein	[more]
AT5G59090.1	1.3e-33	53.97	subtilase 4.12	[more]
AT4G15040.1	2.8e-33	48.57	Subtilisin-like serine endopeptidase family protein	[more]
AT5G58820.1	2.8e-33	48.94	Subtilisin-like serine endopeptidase family protein	[more]
AT5G58840.1	6.3e-33	47.83	Subtilase family protein	[more]

BLAST of CsGy5G007360 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q39547\|CUCM1_CUCME	6.1e-54	71.94	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
sp\|Q9FIF8\|SBT43_ARATH	1.6e-33	49.28	Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S...	[more]
sp\|Q8L7D2\|SBT4C_ARATH	2.3e-32	53.97	Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2...	[more]
sp\|O23357\|SBT42_ARATH	5.1e-32	48.57	Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana OX=3702 GN=SBT4.2 PE=3 S...	[more]
sp\|F4KGD4\|SBT47_ARATH	5.1e-32	48.94	Subtilisin-like protease SBT4.7 OS=Arabidopsis thaliana OX=3702 GN=SBT4.7 PE=3 S...	[more]

BLAST of CsGy5G007360 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KLR1\|A0A0A0KLR1_CUCSA	1.1e-76	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187340 PE=4 SV=1	[more]
tr\|A0A1S3CF95\|A0A1S3CF95_CUCME	1.8e-74	97.12	cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=4 SV=1	[more]
tr\|A0A1S3CFE1\|A0A1S3CFE1_CUCME	3.1e-58	77.70	cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500264 PE=4 SV=1	[more]
tr\|A0A0A0KLR4\|A0A0A0KLR4_CUCSA	8.9e-58	76.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187880 PE=4 SV=1	[more]
tr\|A0A1S3CF99\|A0A1S3CF99_CUCME	2.4e-55	72.66	cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500258 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **Molecular Function**
Term	Definition
GO:0004252	serine-type endopeptidase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR037045	S8pro/Inhibitor_I9_sf
IPR036852	Peptidase_S8/S53_dom_sf
IPR010259	S8pro/Inhibitor_I9

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004252	serine-type endopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy5G007360.1	CsGy5G007360.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 8..69 e-value: 3.4E-13 score: 50.0
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	G3DSA:3.40.50.200		coord: 74..139 e-value: 2.9E-16 score: 61.4
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	SSF52743	Subtilisin-like	coord: 36..133
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	G3DSA:3.30.70.80		coord: 1..73 e-value: 1.1E-24 score: 88.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 111..139
None	No IPR available	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 1..138
None	No IPR available	PANTHER	PTHR10795:SF348	SUBTILISIN-LIKE PROTEASE SBT4.1	coord: 1..138

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsGy5G007360	Csa5G187340	Cucumber (Chinese Long) v2	cgybcuB224
CsGy5G007360	ClCG07G003700	Watermelon (Charleston Gray)	cgybwcgB369
CsGy5G007360	CmoCh04G012280	Cucurbita moschata (Rifu)	cgybcmoB651
CsGy5G007360	MELO3C023899.2	Melon (DHL92) v3.6.1	cgybmedB310
CsGy5G007360	MELO3C023918.2	Melon (DHL92) v3.6.1	cgybmedB312
CsGy5G007360	CsaV3_5G008460	Cucumber (Chinese Long) v3	cgybcucB232
CsGy5G007360	Bhi07G000583	Wax gourd	cgybwgoB487
CsGy5G007360	Bhi06G000880	Wax gourd	cgybwgoB441
CsGy5G007360	Bhi07G000589	Wax gourd	cgybwgoB485
CsGy5G007360	Carg00244	Silver-seed gourd	carcgybB0670
CsGy5G007360	Carg18344	Silver-seed gourd	carcgybB0098

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy5G007360	Cucurbita maxima (Rimu)	cgybcmaB689
CsGy5G007360	Cucumber (Gy14) v2	cgybcgybB092
CsGy5G007360	Cucumber (Gy14) v2	cgybcgybB143
CsGy5G007360	Cucurbita maxima (Rimu)	cgybcmaB645
CsGy5G007360	Cucurbita maxima (Rimu)	cgybcmaB673
CsGy5G007360	Cucurbita maxima (Rimu)	cgybcmaB674
CsGy5G007360	Cucurbita maxima (Rimu)	cgybcmaB697
CsGy5G007360	Cucurbita moschata (Rifu)	cgybcmoB569
CsGy5G007360	Cucurbita moschata (Rifu)	cgybcmoB581
CsGy5G007360	Cucurbita moschata (Rifu)	cgybcmoB623
CsGy5G007360	Cucurbita moschata (Rifu)	cgybcmoB652
CsGy5G007360	Cucurbita moschata (Rifu)	cgybcmoB670
CsGy5G007360	Cucurbita moschata (Rifu)	cgybcmoB677
CsGy5G007360	Cucurbita moschata (Rifu)	cgybcmoB680
CsGy5G007360	Cucurbita pepo (Zucchini)	cgybcpeB591
CsGy5G007360	Cucurbita pepo (Zucchini)	cgybcpeB614
CsGy5G007360	Cucurbita pepo (Zucchini)	cgybcpeB646
CsGy5G007360	Cucurbita pepo (Zucchini)	cgybcpeB647
CsGy5G007360	Cucurbita pepo (Zucchini)	cgybcpeB661
CsGy5G007360	Cucurbita pepo (Zucchini)	cgybcpeB675
CsGy5G007360	Cucurbita pepo (Zucchini)	cgybcpeB701
CsGy5G007360	Cucumber (Chinese Long) v2	cgybcuB212
CsGy5G007360	Cucumber (Chinese Long) v2	cgybcuB235
CsGy5G007360	Bottle gourd (USVL1VR-Ls)	cgyblsiB326
CsGy5G007360	Bottle gourd (USVL1VR-Ls)	cgyblsiB330
CsGy5G007360	Bottle gourd (USVL1VR-Ls)	cgyblsiB331
CsGy5G007360	Melon (DHL92) v3.5.1	cgybmeB342
CsGy5G007360	Melon (DHL92) v3.5.1	cgybmeB310
CsGy5G007360	Melon (DHL92) v3.6.1	cgybmedB341
CsGy5G007360	Watermelon (Charleston Gray)	cgybwcgB320
CsGy5G007360	Watermelon (97103) v1	cgybwmB344
CsGy5G007360	Watermelon (97103) v1	cgybwmB365
CsGy5G007360	Watermelon (97103) v1	cgybwmB407
CsGy5G007360	Wild cucumber (PI 183967)	cgybcpiB214
CsGy5G007360	Wild cucumber (PI 183967)	cgybcpiB226
CsGy5G007360	Wild cucumber (PI 183967)	cgybcpiB241
CsGy5G007360	Silver-seed gourd	carcgybB0279
CsGy5G007360	Silver-seed gourd	carcgybB0452
CsGy5G007360	Silver-seed gourd	carcgybB0986
CsGy5G007360	Cucumber (Chinese Long) v3	cgybcucB223
CsGy5G007360	Cucumber (Chinese Long) v3	cgybcucB240
CsGy5G007360	Watermelon (97103) v2	cgybwmbB346
CsGy5G007360	Watermelon (97103) v2	cgybwmbB375
CsGy5G007360	Wax gourd	cgybwgoB467