CsGy5G004630 (gene) Cucumber (Gy14) v2

Overview
NameCsGy5G004630
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionCytochrome p450
LocationChr5 : 3016213 .. 3017088 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAGAGAGAGAGAGAGAGAGATGTTGGTTGAGATATTTGAAGGTATTGGGTTGGTGATTTTTGGTTTATTTATTTTGTTTGTTTTTAGGGTATTGTTTTGTTGTTGGGTTTCACCATTTTTAGTACATAAAAAGCTTAAGAGAAATGGGTTTAGAGGTCCAACCCCATGTTTTCCTCTTGGAAATATCAGTGAAATGAAAAGGATGAATGCAAACAACCCTAATAGTACAGTTGAAGATTCCTCCTCTCATCATCAACTTACTCACGATATTCATTCCACTGTCTTCCCGTATTTTGCAAGGTGGCAAACTACGTACGGTAAGCATTCTTATATATAAGAGAAACGACATTGTTATATGATTCATTCTTCTTCATTGATTTAATTTTAATTTCCCTCTTTAAATCTTTAGATATTACTAATGTTTTTAGCATTTTCTTTATTGAATAGTTGGACTTCATGCATACGCAGAAGTTGATGTCAGCTGAGTTTATACGTTCATGTCGGTGAATTGTTTTAAGTATTTTCTTATAGAGAAATATACGATTAGATCAGGGATGAATAATCATAATTAGTAGGGGGTGCAATGGAATACTTCAACTGGAATTTTAACGTTGATTGACGATGAATTGTGTATGTTATTGCAGGAAAAGTATTTACATATTGGTTGGGGACAGAGCCATTCTTGTATATAGCAGAACCAGAATTAGTGAAGATATTATCAAAAGAAGTAAAAGCAAAGCATTGGGGAAAGCCATCAGTGTTCAAAAATGACAGAAAATCAATGTTTGGAAATGGTTTGGTAATGGCTGAAGGAGATGAATGGGTTCGCCAAAGACATATCATTACTCCTGCGTTTAATCCTTCCAACTTGAAG

mRNA sequence

GAGAGAGAGAGAGAGAGAGAGATGTTGGTTGAGATATTTGAAGGTATTGGGTTGGTGATTTTTGGTTTATTTATTTTGTTTGTTTTTAGGGTATTGTTTTGTTGTTGGGTTTCACCATTTTTAGTACATAAAAAGCTTAAGAGAAATGGGTTTAGAGGTCCAACCCCATGTTTTCCTCTTGGAAATATCAGTGAAATGAAAAGGATGAATGCAAACAACCCTAATAGTACAGTTGAAGATTCCTCCTCTCATCATCAACTTACTCACGATATTCATTCCACTGTCTTCCCGTATTTTGCAAGGTGGCAAACTACGTACGGAAAAGTATTTACATATTGGTTGGGGACAGAGCCATTCTTGTATATAGCAGAACCAGAATTAGTGAAGATATTATCAAAAGAAGTAAAAGCAAAGCATTGGGGAAAGCCATCAGTGTTCAAAAATGACAGAAAATCAATGTTTGGAAATGGTTTGGTAATGGCTGAAGGAGATGAATGGGTTCGCCAAAGACATATCATTACTCCTGCGTTTAATCCTTCCAACTTGAAG

Coding sequence (CDS)

ATGTTGGTTGAGATATTTGAAGGTATTGGGTTGGTGATTTTTGGTTTATTTATTTTGTTTGTTTTTAGGGTATTGTTTTGTTGTTGGGTTTCACCATTTTTAGTACATAAAAAGCTTAAGAGAAATGGGTTTAGAGGTCCAACCCCATGTTTTCCTCTTGGAAATATCAGTGAAATGAAAAGGATGAATGCAAACAACCCTAATAGTACAGTTGAAGATTCCTCCTCTCATCATCAACTTACTCACGATATTCATTCCACTGTCTTCCCGTATTTTGCAAGGTGGCAAACTACGTACGGAAAAGTATTTACATATTGGTTGGGGACAGAGCCATTCTTGTATATAGCAGAACCAGAATTAGTGAAGATATTATCAAAAGAAGTAAAAGCAAAGCATTGGGGAAAGCCATCAGTGTTCAAAAATGACAGAAAATCAATGTTTGGAAATGGTTTGGTAATGGCTGAAGGAGATGAATGGGTTCGCCAAAGACATATCATTACTCCTGCGTTTAATCCTTCCAACTTGAAG

Protein sequence

MLVEIFEGIGLVIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK
Homology
[Close]
BLAST of CsGy5G004630 vs. NCBI nr
Match: XP_011654827.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis sativus])

HSP 1 Score: 365.2 bits (936), Expect = 1.3e-97
Identity = 175/176 (99.43%), Postives = 175/176 (99.43%), Query Frame = 0 

Query: 1   MLVEIFEGIGLVIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMK 60
           MLVEIFEGIGLVIF LFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMK
Sbjct: 1   MLVEIFEGIGLVIFCLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMK 60

Query: 61  RMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPEL 120
           RMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPEL
Sbjct: 61  RMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPEL 120

Query: 121 VKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
           VKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK
Sbjct: 121 VKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 176

[Close]
BLAST of CsGy5G004630 vs. NCBI nr
Match: XP_016898838.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 323.6 bits (828), Expect = 4.3e-85
Identity = 160/182 (87.91%), Postives = 164/182 (90.11%), Query Frame = 0 

Query: 1   MLVEIFEGIGLVIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEM- 60
           MLVEIFEGIGLVIFGLFILFV RVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEM 
Sbjct: 1   MLVEIFEGIGLVIFGLFILFVLRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMX 60

Query: 61  ---KRMNANNPNSTVEDSSSHHQ--LTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLY 120
                    NPNST EDS SH    +TH+IHSTVFPYFARWQTTYGKVFTYWLGTEPFLY
Sbjct: 61  XXXXXXXXXNPNSTFEDSFSHDDQLITHNIHSTVFPYFARWQTTYGKVFTYWLGTEPFLY 120

Query: 121 IAEPELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSN 177
           IA+PE VKILSKEVKAK+WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSN
Sbjct: 121 IADPEFVKILSKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSN 180

[Close]
BLAST of CsGy5G004630 vs. NCBI nr
Match: XP_022995689.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 268.5 bits (685), Expect = 1.6e-68
Identity = 130/176 (73.86%), Postives = 143/176 (81.25%), Query Frame = 0 

Query: 1   MLVEIFEGIGLVIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMK 60
           MLVE  E IGLVI   FI  +F+VLF CW+SPFL HKKLKRNGFRGPTP FPLGNISEMK
Sbjct: 1   MLVE--EAIGLVIMAFFISLLFKVLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMK 60

Query: 61  RMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPEL 120
           R +                LTHDIHS VFPYF+RWQ  YGK+FTYWLGTEPFLY+A+PE 
Sbjct: 61  RTSFGR---------VFECLTHDIHSIVFPYFSRWQAAYGKIFTYWLGTEPFLYLADPEF 120

Query: 121 VKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
           VK+LS+EV+AK+WGKPSVFK DRKSMFGNGLVMAEGDEWVRQRH+ITPAFNPSNLK
Sbjct: 121 VKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLK 165

[Close]
BLAST of CsGy5G004630 vs. NCBI nr
Match: XP_022958036.1 (cytokinin hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 263.8 bits (673), Expect = 4.0e-67
Identity = 127/176 (72.16%), Postives = 141/176 (80.11%), Query Frame = 0 

Query: 1   MLVEIFEGIGLVIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMK 60
           MLVE  E  GLVI       +F++LF CW+SPFL HKKLKRNGFRGPTP FPLGNISEMK
Sbjct: 1   MLVE--EAFGLVIMAFLTSLLFKILFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMK 60

Query: 61  RMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPEL 120
           R + +              LTHDIHS VFPYF+RWQ  +GKVFTYWLGTEPFLY+AEPE 
Sbjct: 61  RTSFDR---------VFESLTHDIHSIVFPYFSRWQAAHGKVFTYWLGTEPFLYVAEPEF 120

Query: 121 VKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
           VK+LS+EV+AK+WGKPSVFK DRKSMFGNGLVMAEGDEWVRQRH+ITPAFNPSNLK
Sbjct: 121 VKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLK 165

[Close]
BLAST of CsGy5G004630 vs. NCBI nr
Match: XP_022159617.1 (cytokinin hydroxylase-like [Momordica charantia])

HSP 1 Score: 263.5 bits (672), Expect = 5.3e-67
Identity = 127/172 (73.84%), Postives = 143/172 (83.14%), Query Frame = 0 

Query: 5   IFEGIGLVIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKRMNA 64
           +FE I L I  L IL V  VLFCCW+SPFL + KLKRNGF GPTP FPLGNISEMK+   
Sbjct: 2   LFEAINLAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKK--- 61

Query: 65  NNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKIL 124
            N N +V +SS   Q+TH+IHSTVFPYF+RWQ ++GK+F YWLGTEPFLYIAEPE +K L
Sbjct: 62  KNKNCSVLESS---QVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTL 121

Query: 125 SKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
           S EVKAK WGKPSVFK+DRKSMFGNGLVMAEGD+WVRQRH+ITPAFNPSNLK
Sbjct: 122 STEVKAKFWGKPSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLK 167

[Close]
BLAST of CsGy5G004630 vs. TAIR10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1)

HSP 1 Score: 193.0 bits (489), Expect = 1.6e-49
Identity = 90/165 (54.55%), Postives = 114/165 (69.09%), Query Frame = 0 

Query: 13  IFGLFILFVFRVLFC-CWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKRMNANNPNSTV 72
           +F + I  +F  LF  CW+ P    KKL+ NGF GP P FP GN+++MK++     +  V
Sbjct: 16  VFVVVICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKLKM--ASVVV 75

Query: 73  EDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKAK 132
           ++S S   + HDIHS   P+FARWQ  YGKVF YWLG EPF+Y+A+PE + ++SK V  K
Sbjct: 76  DNSKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKGVLGK 135

Query: 133 HWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
            WGKP+VFK DR+ MFG GLVM EGD+W R RHIITPAF P NLK
Sbjct: 136 SWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLK 178

[Close]
BLAST of CsGy5G004630 vs. TAIR10
Match: AT4G27710.1 (cytochrome P450, family 709, subfamily B, polypeptide 3)

HSP 1 Score: 99.8 bits (247), Expect = 1.8e-21
Identity = 58/184 (31.52%), Postives = 94/184 (51.09%), Query Frame = 0 

Query: 3   VEIFEGIGLVIFGLFILFVFRVLFCCWV---SPFLVHKKLKRNGFRGPTPCFPLGNISEM 62
           +E+   I L+   L +  V ++   CW+    P ++ K+ K+ G  GP      GN+SE+
Sbjct: 1   MELISTINLLTIVLLLFVVSKIWKACWILLLRPLMLSKRFKKQGISGPKYKILYGNLSEI 60

Query: 63  KRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPE 122
           K+M        ++ +S      +DI   VFP + +W + YG  F +W GT+P +YI+  E
Sbjct: 61  KKMKKEADLCVLDPNS------NDIFPRVFPQYHQWMSQYGDTFLFWTGTKPTIYISNHE 120

Query: 123 LVKILSKEVKAKHWG-------KPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNP 177
               L+K+V +  +G       +P VF      +FG GL   +GD+W+R R I+ PAF+ 
Sbjct: 121 ----LAKQVLSSKFGFTIIPVKRPEVF-----ILFGKGLSFIQGDDWIRHRRILNPAFSM 169

[Close]
BLAST of CsGy5G004630 vs. TAIR10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1)

HSP 1 Score: 98.2 bits (243), Expect = 5.3e-21
Identity = 59/168 (35.12%), Postives = 88/168 (52.38%), Query Frame = 0 

Query: 9   IGLVIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKRMNANNPN 68
           IGL IF  F++ V+         PF++ ++LK  G  GP      GN+SE+K+M   +  
Sbjct: 16  IGLRIFKAFMILVWH--------PFVLTRRLKNQGISGPNYRIFYGNLSEIKKMKRESHL 75

Query: 69  STVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEV 128
           S ++ SS      +DI   + P++ +W + YG+ F YW GTEP + I++PEL K +    
Sbjct: 76  SILDPSS------NDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTMLSN- 135

Query: 129 KAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
           K   + K        K +   GLV  EG +WVR R I+ PAF+   LK
Sbjct: 136 KLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLK 168

[Close]
BLAST of CsGy5G004630 vs. TAIR10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1)

HSP 1 Score: 94.4 bits (233), Expect = 7.7e-20
Identity = 65/180 (36.11%), Postives = 93/180 (51.67%), Query Frame = 0 

Query: 1   MLVEIFEGIGLVIFGLFILFV-FRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEM 60
           ML+ I + + LVIF   IL V +  + C W++P  + K +++ G  GP P    GNI E+
Sbjct: 1   MLLTILKSL-LVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI 60

Query: 61  KRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPE 120
             M +    S  +D  S H   HDI   + P++  W   YGK F  W GT+P L + E E
Sbjct: 61  SAMVS---QSASKDCDSIH---HDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETE 120

Query: 121 LVKILSKEVKAKH---WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
           L+    KE+  KH    G+  + +   K+  G GL+MA G +W  QRH+  PAF    LK
Sbjct: 121 LI----KELLMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLK 169

[Close]
BLAST of CsGy5G004630 vs. TAIR10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2)

HSP 1 Score: 93.6 bits (231), Expect = 1.3e-19
Identity = 57/179 (31.84%), Postives = 92/179 (51.40%), Query Frame = 0 

Query: 1   MLVEIFEGIGLVIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMK 60
           M+  + + + +++  L +  ++  + C +++P  + K ++R G  GP P    GNI ++ 
Sbjct: 2   MVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDIS 61

Query: 61  RMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPEL 120
           +M +   +S   D SS H   H+I   + P++  W   YGK F  W GTEP L + E E+
Sbjct: 62  KMLS---HSASNDCSSIH---HNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEM 121

Query: 121 VKILSKEVKAKH---WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
           +    KE+  KH    GK  + +   K   G GL+MA G+ W  QRH+  PAF    LK
Sbjct: 122 I----KELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLK 170

[Close]
BLAST of CsGy5G004630 vs. Swiss-Prot
Match: sp|B9G934|C14C3_ORYSJ (Cytochrome P450 714C3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C3 PE=3 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 3.2e-23
Identity = 63/168 (37.50%), Postives = 88/168 (52.38%), Query Frame = 0 

Query: 12  VIFGLFILFVFRVLFCC---WVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKRMNANNPN 71
           +I  L IL    + + C   W+    + KKL+R G RGP P F  GN  E+KR+      
Sbjct: 7   LIVVLVILLSLALFYLCNILWLRAVKIRKKLRRQGIRGPKPTFLYGNTKEIKRIRQ---- 66

Query: 72  STVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEV 131
              E   S  Q T++  ST+FP+F  W+ TYG VF Y  G    L ++ P++VK + +  
Sbjct: 67  ---ELKLSQKQGTNNFISTLFPHFLLWRETYGPVFLYSTGAMEILQVSHPDMVKDIGRWT 126

Query: 132 KAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
            ++  GKP+  K  RK++FG GL    GDEW  QR II P F    +K
Sbjct: 127 PSE-LGKPNYLKKSRKALFGGGLFTENGDEWAYQRKIIAPEFFMDKIK 166

[Close]
BLAST of CsGy5G004630 vs. Swiss-Prot
Match: sp|Q7XHW5|C14B1_ORYSJ (Cytochrome P450 714B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714B1 PE=1 SV=2)

HSP 1 Score: 106.3 bits (264), Expect = 3.5e-22
Identity = 51/152 (33.55%), Postives = 81/152 (53.29%), Query Frame = 0 

Query: 26  FCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMK-RMNANNPNSTVEDSSSHHQLTHDI 85
           +  W++P  +   L+R G  GPTP FP GN+++M+    A        +      + HD 
Sbjct: 24  YVLWLAPERLRAHLRRQGIGGPTPSFPYGNLADMRSHAAAAAGGKATGEGRQEGDIVHDY 83

Query: 86  HSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKAKHWGKPSVFKNDRK 145
              VFP++  W+  YG VFTY +G   FL+++ P++V+ LS  V +   GK S  K   +
Sbjct: 84  RQAVFPFYENWRKQYGPVFTYSVGNMVFLHVSRPDIVRELSLCV-SLDLGKSSYMKATHQ 143

Query: 146 SMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
            +FG G++ + G+ W  QR +I P F P  +K
Sbjct: 144 PLFGEGILKSNGNAWAHQRKLIAPEFFPDKVK 174

[Close]
BLAST of CsGy5G004630 vs. Swiss-Prot
Match: sp|B9GBJ9|C14C1_ORYSJ (Cytochrome P450 714C1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C1 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.3e-21
Identity = 62/168 (36.90%), Postives = 87/168 (51.79%), Query Frame = 0 

Query: 12  VIFGLFILFVFRVLFCC---WVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKRMNANNPN 71
           +I  L IL    + + C   W+    + KKL+R G RGP P F  GN  E+KR+      
Sbjct: 7   LIVVLVILLSLALFYLCNILWLRAVKIRKKLRRQGIRGPKPTFLYGNTKEIKRIRQ---- 66

Query: 72  STVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEV 131
              E   S  Q T++  ST+FP+F   + TYG VF Y  G    L ++ P++VK + +  
Sbjct: 67  ---ELKFSQKQGTNNFISTLFPHFLLCRETYGPVFLYSTGALEILQVSHPDMVKDIGRWT 126

Query: 132 KAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
            ++  GKP+  K  RK++FG GL    GDEW  QR II P F    +K
Sbjct: 127 PSE-LGKPNYLKKSRKALFGGGLFTENGDEWAYQRKIIAPEFFMDKIK 166

[Close]
BLAST of CsGy5G004630 vs. Swiss-Prot
Match: sp|Q9T093|C70B3_ARATH (Cytochrome P450 709B3 OS=Arabidopsis thaliana OX=3702 GN=CYP709B3 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.3e-20
Identity = 58/184 (31.52%), Postives = 94/184 (51.09%), Query Frame = 0 

Query: 3   VEIFEGIGLVIFGLFILFVFRVLFCCWV---SPFLVHKKLKRNGFRGPTPCFPLGNISEM 62
           +E+   I L+   L +  V ++   CW+    P ++ K+ K+ G  GP      GN+SE+
Sbjct: 1   MELISTINLLTIVLLLFVVSKIWKACWILLLRPLMLSKRFKKQGISGPKYKILYGNLSEI 60

Query: 63  KRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPE 122
           K+M        ++ +S      +DI   VFP + +W + YG  F +W GT+P +YI+  E
Sbjct: 61  KKMKKEADLCVLDPNS------NDIFPRVFPQYHQWMSQYGDTFLFWTGTKPTIYISNHE 120

Query: 123 LVKILSKEVKAKHWG-------KPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNP 177
               L+K+V +  +G       +P VF      +FG GL   +GD+W+R R I+ PAF+ 
Sbjct: 121 ----LAKQVLSSKFGFTIIPVKRPEVF-----ILFGKGLSFIQGDDWIRHRRILNPAFSM 169

[Close]
BLAST of CsGy5G004630 vs. Swiss-Prot
Match: sp|Q2QYH7|C14C2_ORYSJ (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 5.6e-20
Identity = 51/153 (33.33%), Postives = 82/153 (53.59%), Query Frame = 0 

Query: 16  LFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKRMNANNPNSTVEDSS 75
           L IL    V    W+ P  + +KL+  G RGP P F  GNI EM+R+     ++  +++ 
Sbjct: 18  LCILLFSYVYIILWLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQEAG 77

Query: 76  SHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKAKHWGK 135
           S    + +  +T+FPYF  W   YG ++ Y  G+   L + +P +VK L+   K+   GK
Sbjct: 78  STDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELA-NCKSLDLGK 137

Query: 136 PSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITP 169
           P   + +R ++ G G++ + GD WV QR +I P
Sbjct: 138 PCYLQKERGALLGMGILTSNGDLWVHQRKVIAP 169

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BLAST of CsGy5G004630 vs. TrEMBL
Match: tr|A0A1S4DS94|A0A1S4DS94_CUCME (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.8e-85
Identity = 160/182 (87.91%), Postives = 164/182 (90.11%), Query Frame = 0 

Query: 1   MLVEIFEGIGLVIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEM- 60
           MLVEIFEGIGLVIFGLFILFV RVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEM 
Sbjct: 1   MLVEIFEGIGLVIFGLFILFVLRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMX 60

Query: 61  ---KRMNANNPNSTVEDSSSHHQ--LTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLY 120
                    NPNST EDS SH    +TH+IHSTVFPYFARWQTTYGKVFTYWLGTEPFLY
Sbjct: 61  XXXXXXXXXNPNSTFEDSFSHDDQLITHNIHSTVFPYFARWQTTYGKVFTYWLGTEPFLY 120

Query: 121 IAEPELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSN 177
           IA+PE VKILSKEVKAK+WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSN
Sbjct: 121 IADPEFVKILSKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSN 180

[Close]
BLAST of CsGy5G004630 vs. TrEMBL
Match: tr|E5GCU7|E5GCU7_CUCME (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.0e-66
Identity = 128/153 (83.66%), Postives = 132/153 (86.27%), Query Frame = 0 

Query: 30  VSPFLVHKKLKRNGFRGPTPCFPLGNISEM----KRMNANNPNSTVEDSSSHHQ--LTHD 89
           V  F VHKKLKRNGFRGPTPCFPLGNISEM           PNST EDS SH    +TH+
Sbjct: 3   VEIFEVHKKLKRNGFRGPTPCFPLGNISEMXXXXXXXXXXXPNSTFEDSFSHDDQLITHN 62

Query: 90  IHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKAKHWGKPSVFKNDR 149
           IHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA+PE VKILSKEVKAK+WGKPSVFKNDR
Sbjct: 63  IHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIADPEFVKILSKEVKAKNWGKPSVFKNDR 122

Query: 150 KSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
           KSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK
Sbjct: 123 KSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 155

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BLAST of CsGy5G004630 vs. TrEMBL
Match: tr|A0A0A0KKS5|A0A0A0KKS5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166390 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.4e-62
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 0 

Query: 62  MNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELV 121
           MNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELV
Sbjct: 1   MNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELV 60

Query: 122 KILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
           KILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK
Sbjct: 61  KILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 115

[Close]
BLAST of CsGy5G004630 vs. TrEMBL
Match: tr|A0A2I4FGE1|A0A2I4FGE1_9ROSI (cytokinin hydroxylase-like OS=Juglans regia OX=51240 GN=LOC108998581 PE=3 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 9.5e-57
Identity = 106/173 (61.27%), Postives = 131/173 (75.72%), Query Frame = 0 

Query: 4   EIFEGIGLVIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKRMN 63
           E+F    + +   F+   +R+LF CW+SP  V++KL+RNGF GPTP FPLGNI+EM + N
Sbjct: 7   ELFRAFSIAMVAFFLFLTWRILFSCWISPTQVYQKLRRNGFGGPTPSFPLGNITEMTKKN 66

Query: 64  ANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKI 123
                S++  S S     HDIHS+VFPYFARWQ ++GKVF YWLGTEPFLYIA+PE +K+
Sbjct: 67  G---GSSLGPSKS----AHDIHSSVFPYFARWQKSHGKVFVYWLGTEPFLYIADPEFLKM 126

Query: 124 LSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
           +S EV AK WGKPSVF+NDR  MFGNGLVM EGD WVR RH+ITPAF+P+NLK
Sbjct: 127 MSAEVVAKSWGKPSVFRNDRDPMFGNGLVMIEGDNWVRHRHVITPAFHPTNLK 172

[Close]
BLAST of CsGy5G004630 vs. TrEMBL
Match: tr|A0A058ZYM0|A0A058ZYM0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_K00273 PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.8e-55
Identity = 102/165 (61.82%), Postives = 127/165 (76.97%), Query Frame = 0 

Query: 12  VIFGLFILFVFRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKRMNANNPNSTV 71
           V+F LF+L  +RV F CW+SP   ++KLKRNGF+GP+P FPLGNI +MK+       S  
Sbjct: 16  VVFALFVL--WRVSFRCWISPARAYQKLKRNGFKGPSPVFPLGNIMDMKKAIEEGSRSVN 75

Query: 72  EDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKAK 131
            +  S + +THDIHSTVFPY+ARWQ ++GKVF YWLGTEPFLY+A+PE +K +S  V  K
Sbjct: 76  SNDESLNTITHDIHSTVFPYYARWQKSHGKVFVYWLGTEPFLYVADPEFLKQMSSGVMGK 135

Query: 132 HWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 177
            WGKP VFKNDR+ MFGNGLVM EGD+WV  RH+ITPAF+P+NLK
Sbjct: 136 SWGKPRVFKNDREPMFGNGLVMTEGDQWVHHRHVITPAFSPANLK 178

The following BLAST results are available for this feature:
BLAST of CsGy5G004630 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011654827.11.3e-9799.43PREDICTED: cytokinin hydroxylase-like [Cucumis sativus][more]
XP_016898838.14.3e-8587.91PREDICTED: cytokinin hydroxylase-like [Cucumis melo][more]
XP_022995689.11.6e-6873.86cytokinin hydroxylase-like [Cucurbita maxima][more]
XP_022958036.14.0e-6772.16cytokinin hydroxylase-like [Cucurbita moschata][more]
XP_022159617.15.3e-6773.84cytokinin hydroxylase-like [Momordica charantia][more]
BLAST of CsGy5G004630 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G52400.11.6e-4954.55cytochrome P450, family 715, subfamily A, polypeptide 1[more]
AT4G27710.11.8e-2131.52cytochrome P450, family 709, subfamily B, polypeptide 3[more]
AT2G46960.25.3e-2135.12cytochrome P450, family 709, subfamily B, polypeptide 1[more]
AT5G38450.17.7e-2036.11cytochrome P450, family 735, subfamily A, polypeptide 1[more]
AT1G67110.11.3e-1931.84cytochrome P450, family 735, subfamily A, polypeptide 2[more]
BLAST of CsGy5G004630 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|B9G934|C14C3_ORYSJ3.2e-2337.50Cytochrome P450 714C3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C3 PE=3 ... [more]
sp|Q7XHW5|C14B1_ORYSJ3.5e-2233.55Cytochrome P450 714B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714B1 PE=1 ... [more]
sp|B9GBJ9|C14C1_ORYSJ1.3e-2136.90Cytochrome P450 714C1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C1 PE=2 ... [more]
sp|Q9T093|C70B3_ARATH3.3e-2031.52Cytochrome P450 709B3 OS=Arabidopsis thaliana OX=3702 GN=CYP709B3 PE=2 SV=1[more]
sp|Q2QYH7|C14C2_ORYSJ5.6e-2033.33Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... [more]
BLAST of CsGy5G004630 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S4DS94|A0A1S4DS94_CUCME2.8e-8587.91cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1[more]
tr|E5GCU7|E5GCU7_CUCME1.0e-6683.66Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
tr|A0A0A0KKS5|A0A0A0KKS5_CUCSA3.4e-62100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166390 PE=3 SV=1[more]
tr|A0A2I4FGE1|A0A2I4FGE1_9ROSI9.5e-5761.27cytokinin hydroxylase-like OS=Juglans regia OX=51240 GN=LOC108998581 PE=3 SV=1[more]
tr|A0A058ZYM0|A0A058ZYM0_EUCGR1.8e-5561.82Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_K00273 PE=3 SV... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037heme binding
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR036396Cyt_P450_sf
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G004630.1CsGy5G004630.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036396Cytochrome P450 superfamilyGENE3DG3DSA:1.10.630.10coord: 33..176
e-value: 4.2E-17
score: 63.8
IPR036396Cytochrome P450 superfamilySUPERFAMILYSSF48264Cytochrome P450coord: 39..176
IPR001128Cytochrome P450PFAMPF00067p450coord: 82..172
e-value: 1.8E-10
score: 40.1
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 17..176
NoneNo IPR availablePANTHERPTHR24282:SF15CYTOCHROME P450, FAMILY 715, SUBFAMILY A, POLYPEPTIDE 1coord: 17..176