CsGy5G001530 (gene) Cucumber (Gy14) v2

Overview
NameCsGy5G001530
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
Descriptionmethionine gamma-lyase-like
LocationChr5 : 957826 .. 958895 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGAGCTAGATCCAGCGGCCGCTCTGGCAATGGCCAGCCCCGAGTTCGGAGAGCATGGTGGCGTGAACATGTCAATTGAAAACTCCATAACATTCACGGTGATGGAGGCGGAGACGATGCGTAGGATGTTTGAAGGGGAGTTGGGGCCTGAGCGGGACTTCTTCATCTACAGCCGCCACTTCAACCCGACCGTCATGGCCCTTAGCCGGCAGATGGCGGCATTGGAAGGGACCGAGGCAGCCTATTGTACGTCGAGTGGGATGGCCGCTGTTTCATCTGTTTTGCTGCAGCTTGTGAGCTCCGGTGGGCATATCGTGGCGTCGAGGACGCTCTACGGCGGTACTCATGCATTGCTGTCGCATTTTCTGCCGAGGGTAAGCAAGATAACAACGACTTTTGTGGACGTGAGGAACCATGAAATGGTTAGAGATGCCATGGTAGAGGGAAAGACGAAGGTACATAATCTAACACCTACTAAAAGTTTTAGGTTTATATATGTTTCCTTGATTGAATTATGAATTTGGTTCTTACACTGTCATTTTCCTGGGGAGAAATTTTACCCTATCAATTTGATTTTTATAATGATTCAAATGTATTTAATTTAATTTTAGTTTTGGTTTGATTTATTTTCATATCGATTAACTTTCTTTTAACGCGTTAATTCGAATCATGGATGACAATTAACACAAAAGGATTGATAATTAAACATTAATAAGAAAAAAACTTAATTATTTTCAAACGTTTGAATCCGAGTTCTTCCTTTATATAACAAGTTAAATCAATTTTTCAAAATTAATTTAGGTTTATAGGTTACAAAGAAATTAAATATTTTACCCTTTTGTTGAACCTATAGGTATTGTATTTTGAGACGGTGGCAAACCCGACGCTTACGGTGGCAAACATCCCCGAGCTCAGTCGGATGGCACACCAGAAGGGGGTGACTGTGGTAGTCGATAATACGTTCTCACCCATAATCATCTCACCGACAAGGCTCGGTGCTGACGTCGTCGTCCATAGCTGCTCAAAGTTTATCAGCGGTGGTGCAGACATTATTGCAGGTATATGA

mRNA sequence

ATGGGTGAGCTAGATCCAGCGGCCGCTCTGGCAATGGCCAGCCCCGAGTTCGGAGAGCATGGTGGCGTGAACATGTCAATTGAAAACTCCATAACATTCACGGTGATGGAGGCGGAGACGATGCGTAGGATGTTTGAAGGGGAGTTGGGGCCTGAGCGGGACTTCTTCATCTACAGCCGCCACTTCAACCCGACCGTCATGGCCCTTAGCCGGCAGATGGCGGCATTGGAAGGGACCGAGGCAGCCTATTGTACGTCGAGTGGGATGGCCGCTGTTTCATCTGTTTTGCTGCAGCTTGTGAGCTCCGGTGGGCATATCGTGGCGTCGAGGACGCTCTACGGCGGTACTCATGCATTGCTGTCGCATTTTCTGCCGAGGGTATTGTATTTTGAGACGGTGGCAAACCCGACGCTTACGGTGGCAAACATCCCCGAGCTCAGTCGGATGGCACACCAGAAGGGGGTGACTGTGGTAGTCGATAATACGTTCTCACCCATAATCATCTCACCGACAAGGCTCGGTGCTGACGTCGTCGTCCATAGCTGCTCAAAGTTTATCAGCGGTGGTGCAGACATTATTGCAGGTATATGA

Coding sequence (CDS)

ATGGGTGAGCTAGATCCAGCGGCCGCTCTGGCAATGGCCAGCCCCGAGTTCGGAGAGCATGGTGGCGTGAACATGTCAATTGAAAACTCCATAACATTCACGGTGATGGAGGCGGAGACGATGCGTAGGATGTTTGAAGGGGAGTTGGGGCCTGAGCGGGACTTCTTCATCTACAGCCGCCACTTCAACCCGACCGTCATGGCCCTTAGCCGGCAGATGGCGGCATTGGAAGGGACCGAGGCAGCCTATTGTACGTCGAGTGGGATGGCCGCTGTTTCATCTGTTTTGCTGCAGCTTGTGAGCTCCGGTGGGCATATCGTGGCGTCGAGGACGCTCTACGGCGGTACTCATGCATTGCTGTCGCATTTTCTGCCGAGGGTATTGTATTTTGAGACGGTGGCAAACCCGACGCTTACGGTGGCAAACATCCCCGAGCTCAGTCGGATGGCACACCAGAAGGGGGTGACTGTGGTAGTCGATAATACGTTCTCACCCATAATCATCTCACCGACAAGGCTCGGTGCTGACGTCGTCGTCCATAGCTGCTCAAAGTTTATCAGCGGTGGTGCAGACATTATTGCAGGTATATGA

Protein sequence

MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAGI
Homology
[Close]
BLAST of CsGy5G001530 vs. NCBI nr
Match: KGN49981.1 (hypothetical protein Csa_5G148520 [Cucumis sativus])

HSP 1 Score: 364.8 bits (935), Expect = 1.9e-97
Identity = 195/222 (87.84%), Postives = 195/222 (87.84%), Query Frame = 0 

Query: 1   MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR 60
           MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR
Sbjct: 37  MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR 96

Query: 61  HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL 120
           HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL
Sbjct: 97  HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL 156

Query: 121 SHFLPR---------------------------VLYFETVANPTLTVANIPELSRMAHQK 180
           SHFLPR                           VLYFETVANPTLTVANIPELSRMAHQK
Sbjct: 157 SHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQK 216

Query: 181 GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG
Sbjct: 217 GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 258

[Close]
BLAST of CsGy5G001530 vs. NCBI nr
Match: XP_004143943.2 (PREDICTED: methionine gamma-lyase-like [Cucumis sativus])

HSP 1 Score: 364.8 bits (935), Expect = 1.9e-97
Identity = 195/222 (87.84%), Postives = 195/222 (87.84%), Query Frame = 0 

Query: 1   MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR 60
           MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR
Sbjct: 1   MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR 60

Query: 61  HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL 120
           HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL
Sbjct: 61  HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL 120

Query: 121 SHFLPR---------------------------VLYFETVANPTLTVANIPELSRMAHQK 180
           SHFLPR                           VLYFETVANPTLTVANIPELSRMAHQK
Sbjct: 121 SHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQK 180

Query: 181 GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG
Sbjct: 181 GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 222

[Close]
BLAST of CsGy5G001530 vs. NCBI nr
Match: XP_008437446.1 (PREDICTED: methionine gamma-lyase-like [Cucumis melo])

HSP 1 Score: 355.9 bits (912), Expect = 8.7e-95
Identity = 189/222 (85.14%), Postives = 193/222 (86.94%), Query Frame = 0 

Query: 1   MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR 60
           MG++DPAAALAMA PEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR
Sbjct: 37  MGKVDPAAALAMARPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR 96

Query: 61  HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL 120
           HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL
Sbjct: 97  HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL 156

Query: 121 SHFLPR---------------------------VLYFETVANPTLTVANIPELSRMAHQK 180
           SHFLPR                           VLYFETVANPTLTVANIPELSRMAH+K
Sbjct: 157 SHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHEK 216

Query: 181 GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           GVTVVVDNTFSP+IISP RLGADVVVHSCSKFISGGADIIAG
Sbjct: 217 GVTVVVDNTFSPMIISPARLGADVVVHSCSKFISGGADIIAG 258

[Close]
BLAST of CsGy5G001530 vs. NCBI nr
Match: XP_023520565.1 (methionine gamma-lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 338.2 bits (866), Expect = 1.9e-89
Identity = 180/221 (81.45%), Postives = 187/221 (84.62%), Query Frame = 0 

Query: 2   GELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRH 61
           GE DPAA+LA A PEFGEHGGVN+SIENSITFTVMEAE MR+MFEGELG E+DFFIYSRH
Sbjct: 31  GEEDPAASLAAARPEFGEHGGVNLSIENSITFTVMEAEMMRKMFEGELGLEQDFFIYSRH 90

Query: 62  FNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLS 121
           FNPTVMAL R MAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLS
Sbjct: 91  FNPTVMALGRAMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLS 150

Query: 122 HFLPR---------------------------VLYFETVANPTLTVANIPELSRMAHQKG 181
           HFLPR                           VLYFETVANPTLTVAN+PELSRMAH+KG
Sbjct: 151 HFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANVPELSRMAHEKG 210

Query: 182 VTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           VTVVVDNTFSP+IISPTRLGADVVVHSCSKFISGGADIIAG
Sbjct: 211 VTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAG 251

[Close]
BLAST of CsGy5G001530 vs. NCBI nr
Match: XP_022970043.1 (methionine gamma-lyase-like [Cucurbita maxima])

HSP 1 Score: 337.8 bits (865), Expect = 2.5e-89
Identity = 179/220 (81.36%), Postives = 187/220 (85.00%), Query Frame = 0 

Query: 3   ELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHF 62
           E DPAA+LA   PEFGEHGGVN+SIENSI+FTVMEAETMR+MFEGELGPE+D FIYSRHF
Sbjct: 28  EEDPAASLAAVRPEFGEHGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYSRHF 87

Query: 63  NPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSH 122
           NPTVMAL RQMAALEGTEAAYCTSSGMAAVSSVLLQL+SSGGHIVASRTLYGGTHALLSH
Sbjct: 88  NPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHALLSH 147

Query: 123 FLPR---------------------------VLYFETVANPTLTVANIPELSRMAHQKGV 182
           FLPR                           VLYFETVANPTLTVANIPELSRMAH+KGV
Sbjct: 148 FLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHEKGV 207

Query: 183 TVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           TVVVDNTFSP+IISPTRLGADVVVHSCSKFISGGADIIAG
Sbjct: 208 TVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAG 247

[Close]
BLAST of CsGy5G001530 vs. TAIR10
Match: AT1G64660.1 (methionine gamma-lyase)

HSP 1 Score: 304.3 bits (778), Expect = 5.4e-83
Identity = 157/220 (71.36%), Postives = 178/220 (80.91%), Query Frame = 0 

Query: 3   ELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHF 62
           + DPAAA+A    EFGEHGGVNMSIE S TFTVME +TMRRMF GELGP+ DF++YSRHF
Sbjct: 40  DADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDNDFYVYSRHF 99

Query: 63  NPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSH 122
           NPTV+ LSRQMAALEGT+AAYCTSSGM+A+SSV+LQL SSGGH+VA+ TLYGGTHALLSH
Sbjct: 100 NPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSH 159

Query: 123 FLPR---------------------------VLYFETVANPTLTVANIPELSRMAHQKGV 182
           FLPR                           VLYFE+VANPTLTVA+IPELSRMAH+KGV
Sbjct: 160 FLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGV 219

Query: 183 TVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           TVVVDNTF+P+++SP +LGADVVVHS SKFISGGADIIAG
Sbjct: 220 TVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAG 259

[Close]
BLAST of CsGy5G001530 vs. TAIR10
Match: AT3G57050.1 (cystathionine beta-lyase)

HSP 1 Score: 103.6 bits (257), Expect = 1.4e-22
Identity = 65/194 (33.51%), Postives = 109/194 (56.19%), Query Frame = 0 

Query: 29  NSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSG 88
           ++++  + +  T ++    E GP    + Y+R  NPT  AL   +A L+  + A+C +SG
Sbjct: 102 DAMSTPLYQTATFKQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSG 161

Query: 89  MAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPR---------------------- 148
           MAA+S+V   L+ +G  IVA   +YGG+  LLS  +PR                      
Sbjct: 162 MAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVAAAIGP 221

Query: 149 ---VLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNT-FSPIIISPTRLGADVVVHSC 197
              +++ E+  NP   +++I ++S MAH +G  V+VDN+  SP++  P  LGAD+V+HS 
Sbjct: 222 QTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSA 281

[Close]
BLAST of CsGy5G001530 vs. TAIR10
Match: AT3G01120.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 94.7 bits (234), Expect = 6.6e-20
Identity = 59/167 (35.33%), Postives = 90/167 (53.89%), Query Frame = 0 

Query: 56  FIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGG 115
           F Y R+ NPT + L  +++ALEG E+    +SGM A + +LL LV +GGHIV +   Y  
Sbjct: 224 FEYGRYGNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRK 283

Query: 116 THALLSHFLPRV-------------------------LYF-ETVANPTLTVANIPELSRM 175
           T   + +FLP++                         L+F E+  NP L   +I  +S++
Sbjct: 284 TRIFMENFLPKLGITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSKI 343

Query: 176 AHQKGVTVVVDNTF-SPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
            H++G  V +D TF +P+      LGAD+VVHS +K+I G  D++AG
Sbjct: 344 CHKRGTLVCIDGTFATPLNQKALALGADLVVHSATKYIGGHNDVLAG 390

[Close]
BLAST of CsGy5G001530 vs. TAIR10
Match: AT1G33320.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 89.0 bits (219), Expect = 3.6e-18
Identity = 58/169 (34.32%), Postives = 89/169 (52.66%), Query Frame = 0 

Query: 54  DFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLY 113
           D + Y+R+ NPT MAL  +++ LEG E+    +SGM A + +LL LV + GHIVA++  Y
Sbjct: 72  DLYEYARYGNPTTMALEEKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCY 131

Query: 114 GGTHALLSHFLPRV-------------------------LYF-ETVANPTLTVANIPELS 173
             T   + +FL ++                         L+F E+  NP L   +I  +S
Sbjct: 132 KETRIFMENFLTKLGITVTFIDSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLVS 191

Query: 174 RMAHQKGVTVVVDNTF-SPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           ++ H++G  V +D T  +PI      LGAD+V HS +K+I G  D +AG
Sbjct: 192 KICHRRGTLVCIDATIATPINQKTLALGADLVHHSATKYIGGHNDFLAG 240

[Close]
BLAST of CsGy5G001530 vs. Swiss-Prot
Match: sp|Q9SGU9|MGL_ARATH (Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 9.8e-82
Identity = 157/220 (71.36%), Postives = 178/220 (80.91%), Query Frame = 0 

Query: 3   ELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHF 62
           + DPAAA+A    EFGEHGGVNMSIE S TFTVME +TMRRMF GELGP+ DF++YSRHF
Sbjct: 40  DADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDNDFYVYSRHF 99

Query: 63  NPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSH 122
           NPTV+ LSRQMAALEGT+AAYCTSSGM+A+SSV+LQL SSGGH+VA+ TLYGGTHALLSH
Sbjct: 100 NPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSH 159

Query: 123 FLPR---------------------------VLYFETVANPTLTVANIPELSRMAHQKGV 182
           FLPR                           VLYFE+VANPTLTVA+IPELSRMAH+KGV
Sbjct: 160 FLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGV 219

Query: 183 TVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           TVVVDNTF+P+++SP +LGADVVVHS SKFISGGADIIAG
Sbjct: 220 TVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAG 259

[Close]
BLAST of CsGy5G001530 vs. Swiss-Prot
Match: sp|Q8L0X4|MEGL_FUSNP (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 GN=mgl PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.4e-27
Identity = 82/204 (40.20%), Postives = 112/204 (54.90%), Query Frame = 0 

Query: 20  HGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGT 79
           +G + M I  + TF    AE   R F      E   +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRF----ALEEAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 80  EAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL--------------- 139
           EAA  TSSGM A+SS L  ++ +G H+V  +TLYG T AL+ H L               
Sbjct: 82  EAAVATSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMCHGLTRFGIEVTFVDTSNL 141

Query: 140 ----------PRVLYFETVANPTLTVANIPELSRMAHQKGVT-VVVDNTF-SPIIISPTR 197
                      RV+Y ET ANP L + ++  LS++AH    T V+VDNTF +P +  P +
Sbjct: 142 DEVKNAMKKNTRVVYLETPANPNLKIVDLEALSKLAHTNPNTLVIVDNTFATPYMQKPLK 201

[Close]
BLAST of CsGy5G001530 vs. Swiss-Prot
Match: sp|Q8RDT4|MEGL_FUSNN (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) OX=190304 GN=FN1419 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.9e-26
Identity = 78/204 (38.24%), Postives = 110/204 (53.92%), Query Frame = 0 

Query: 20  HGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGT 79
           +G + M I  + TF    AE   R F      E   +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRF----ALEEAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 80  EAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL--------------- 139
           EA    SSGM A+SS L  ++ +G H+V  +TLYG T AL++H L               
Sbjct: 82  EAGIAMSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMNHGLTRFGVEVTFVDTSNL 141

Query: 140 ----------PRVLYFETVANPTLTVANIPELSRMAHQKGVT-VVVDNTF-SPIIISPTR 197
                      RV+Y ET ANP L + ++  LS++AH    T V+VDNTF +P +  P +
Sbjct: 142 EEVKNAMKKNTRVVYLETPANPNLKIVDLEALSKIAHTNPNTLVIVDNTFATPYMQKPLK 201

[Close]
BLAST of CsGy5G001530 vs. Swiss-Prot
Match: sp|Q7MX71|MEGL_PORGI (L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=mgl PE=1 SV=2)

HSP 1 Score: 117.1 bits (292), Expect = 2.2e-25
Identity = 77/196 (39.29%), Postives = 107/196 (54.59%), Query Frame = 0 

Query: 27  IENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTS 86
           I  + TF    AE   R F G    E D +IY+R  NP    +  ++A LEG EAA   S
Sbjct: 32  IYQTSTFVFDTAEQGGRRFAG----EEDGYIYTRLGNPNCTQVEEKLAMLEGGEAAASAS 91

Query: 87  SGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPR-------------------- 146
           SG+ A+SS +   V +G HIVA +TLYG T A L+H L R                    
Sbjct: 92  SGIGAISSAIWVCVKAGDHIVAGKTLYGCTFAFLTHGLSRYGVEVTLVDTRHPEEVEAAI 151

Query: 147 -----VLYFETVANPTLTVANIPELSRMAHQ-KGVTVVVDNTF-SPIIISPTRLGADVVV 196
                ++Y ET ANP + + +I  +  +AH+ +GV V+VDNT+ +P I  P  LGAD+VV
Sbjct: 152 RPNTKLVYLETPANPNMYLTDIKAVCDIAHKHEGVRVMVDNTYCTPYICRPLELGADIVV 211

[Close]
BLAST of CsGy5G001530 vs. Swiss-Prot
Match: sp|P13254|MEGL_PSEPU (L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 2.9e-25
Identity = 77/205 (37.56%), Postives = 108/205 (52.68%), Query Frame = 0 

Query: 19  EHGGVNM-SIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALE 78
           +HGG  +  +  + TFT    E     F GE         YSR  NPT+  L  +MA+LE
Sbjct: 23  DHGGALVPPVYQTATFTFPTVEYGAACFAGEQAGH----FYSRISNPTLNLLEARMASLE 82

Query: 79  GTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL------------- 138
           G EA    +SGM A++S L  L+  G  ++   TLYG T A L H +             
Sbjct: 83  GGEAGLALASGMGAITSTLWTLLRPGDEVLLGNTLYGCTFAFLHHGIGEFGVKLRHVDMA 142

Query: 139 ------------PRVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTF-SPIIISPT 197
                        RV+YFE+ ANP + +A+I  ++++A + G TVVVDNT+ +P +  P 
Sbjct: 143 DLQALEAAMTPATRVIYFESPANPNMHMADIAGVAKIARKHGATVVVDNTYCTPYLQRPL 202

[Close]
BLAST of CsGy5G001530 vs. TrEMBL
Match: tr|A0A0A0KQL7|A0A0A0KQL7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.2e-97
Identity = 195/222 (87.84%), Postives = 195/222 (87.84%), Query Frame = 0 

Query: 1   MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR 60
           MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR
Sbjct: 37  MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR 96

Query: 61  HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL 120
           HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL
Sbjct: 97  HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL 156

Query: 121 SHFLPR---------------------------VLYFETVANPTLTVANIPELSRMAHQK 180
           SHFLPR                           VLYFETVANPTLTVANIPELSRMAHQK
Sbjct: 157 SHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQK 216

Query: 181 GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG
Sbjct: 217 GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 258

[Close]
BLAST of CsGy5G001530 vs. TrEMBL
Match: tr|A0A1S3AU15|A0A1S3AU15_CUCME (methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 5.8e-95
Identity = 189/222 (85.14%), Postives = 193/222 (86.94%), Query Frame = 0 

Query: 1   MGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR 60
           MG++DPAAALAMA PEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR
Sbjct: 37  MGKVDPAAALAMARPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSR 96

Query: 61  HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL 120
           HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL
Sbjct: 97  HFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALL 156

Query: 121 SHFLPR---------------------------VLYFETVANPTLTVANIPELSRMAHQK 180
           SHFLPR                           VLYFETVANPTLTVANIPELSRMAH+K
Sbjct: 157 SHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHEK 216

Query: 181 GVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           GVTVVVDNTFSP+IISP RLGADVVVHSCSKFISGGADIIAG
Sbjct: 217 GVTVVVDNTFSPMIISPARLGADVVVHSCSKFISGGADIIAG 258

[Close]
BLAST of CsGy5G001530 vs. TrEMBL
Match: tr|A0A061G217|A0A061G217_THECC (Methionine gamma-lyase OS=Theobroma cacao OX=3641 GN=TCM_015649 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.6e-81
Identity = 162/220 (73.64%), Postives = 179/220 (81.36%), Query Frame = 0 

Query: 3   ELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHF 62
           E DPAAALA A  EFGEHGGVNMSIE S TFTVME ETMRRMF GELGP+RDFFIYSRHF
Sbjct: 44  ENDPAAALASARHEFGEHGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHF 103

Query: 63  NPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSH 122
           NPTV+ L RQMAALEGTEAAYCT+SGM+A+SSVLLQL SSGGH+VASRTLYGGTHALL+H
Sbjct: 104 NPTVLNLGRQMAALEGTEAAYCTASGMSAISSVLLQLCSSGGHVVASRTLYGGTHALLTH 163

Query: 123 FLPR---------------------------VLYFETVANPTLTVANIPELSRMAHQKGV 182
           FLPR                           VLYFE+++NPTLTVANIPELSR+AH+KG 
Sbjct: 164 FLPRACGITTTFVDVCDHEAVRNAIVGGRTKVLYFESMSNPTLTVANIPELSRIAHEKGT 223

Query: 183 TVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           TVVVDNTF+P+++SP RLGADVVVHS SKFISGGAD+IAG
Sbjct: 224 TVVVDNTFAPMVLSPARLGADVVVHSISKFISGGADVIAG 263

[Close]
BLAST of CsGy5G001530 vs. TrEMBL
Match: tr|B9GK77|B9GK77_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_001G084000v3 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.1e-81
Identity = 163/218 (74.77%), Postives = 177/218 (81.19%), Query Frame = 0 

Query: 5   DPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNP 64
           DPAAALA A  EFGEHGGVNMSIE S TFTVME ETMRRMF GELGP+RDFFIYSRHFNP
Sbjct: 47  DPAAALASARHEFGEHGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNP 106

Query: 65  TVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL 124
           TV+ L RQMAALEGTEAAYCT+SGM+A+SSVLLQL SSGGH+VASRTLYGGTHALL+HFL
Sbjct: 107 TVLNLGRQMAALEGTEAAYCTASGMSAISSVLLQLCSSGGHMVASRTLYGGTHALLTHFL 166

Query: 125 PR---------------------------VLYFETVANPTLTVANIPELSRMAHQKGVTV 184
           PR                           VLYFE+++NPTLTVANIPELSR+AH KG TV
Sbjct: 167 PRACNITTTFVDINDHEMVKNAMVEGQTKVLYFESISNPTLTVANIPELSRIAHDKGATV 226

Query: 185 VVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           VVDNTFSP+++SP RLGADVVVHS SKFISGGADIIAG
Sbjct: 227 VVDNTFSPMVLSPARLGADVVVHSISKFISGGADIIAG 264

[Close]
BLAST of CsGy5G001530 vs. TrEMBL
Match: tr|V4SX16|V4SX16_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10020136mg PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.6e-81
Identity = 161/218 (73.85%), Postives = 177/218 (81.19%), Query Frame = 0 

Query: 5   DPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNP 64
           DPAAALA A  EFGEHGGVNMSIE S TFTVME ETMRRMF GELGP+RDFFIYSRHFNP
Sbjct: 40  DPAAALASARHEFGEHGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNP 99

Query: 65  TVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL 124
           TV+ LSRQMAALEGTEAAYCT+SGM+A+SSVL+QL SSGGH+VA+RTLYGGTHALL+HF 
Sbjct: 100 TVLNLSRQMAALEGTEAAYCTASGMSAISSVLMQLCSSGGHVVAARTLYGGTHALLTHFF 159

Query: 125 PR---------------------------VLYFETVANPTLTVANIPELSRMAHQKGVTV 184
           PR                           VLYFE+++NPTL VANIPELSR+AH KGVTV
Sbjct: 160 PRACNITTTFVDISDHEMVNSAIVQGRTKVLYFESISNPTLAVANIPELSRLAHDKGVTV 219

Query: 185 VVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAG 196
           VVDNTFSP+++SP RLGADVVVHS SKFISGGADIIAG
Sbjct: 220 VVDNTFSPLVLSPARLGADVVVHSISKFISGGADIIAG 257

The following BLAST results are available for this feature:
BLAST of CsGy5G001530 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN49981.11.9e-9787.84hypothetical protein Csa_5G148520 [Cucumis sativus][more]
XP_004143943.21.9e-9787.84PREDICTED: methionine gamma-lyase-like [Cucumis sativus][more]
XP_008437446.18.7e-9585.14PREDICTED: methionine gamma-lyase-like [Cucumis melo][more]
XP_023520565.11.9e-8981.45methionine gamma-lyase-like [Cucurbita pepo subsp. pepo][more]
XP_022970043.12.5e-8981.36methionine gamma-lyase-like [Cucurbita maxima][more]
BLAST of CsGy5G001530 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G64660.15.4e-8371.36methionine gamma-lyase[more]
AT3G57050.11.4e-2233.51cystathionine beta-lyase[more]
AT3G01120.16.6e-2035.33Pyridoxal phosphate (PLP)-dependent transferases superfamily protein[more]
AT1G33320.13.6e-1834.32Pyridoxal phosphate (PLP)-dependent transferases superfamily protein[more]
BLAST of CsGy5G001530 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9SGU9|MGL_ARATH9.8e-8271.36Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1[more]
sp|Q8L0X4|MEGL_FUSNP1.4e-2740.20L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 ... [more]
sp|Q8RDT4|MEGL_FUSNN5.9e-2638.24L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATC... [more]
sp|Q7MX71|MEGL_PORGI2.2e-2539.29L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83)... [more]
sp|P13254|MEGL_PSEPU2.9e-2537.56L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2[more]
BLAST of CsGy5G001530 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0KQL7|A0A0A0KQL7_CUCSA1.2e-9787.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1[more]
tr|A0A1S3AU15|A0A1S3AU15_CUCME5.8e-9585.14methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1[more]
tr|A0A061G217|A0A061G217_THECC1.6e-8173.64Methionine gamma-lyase OS=Theobroma cacao OX=3641 GN=TCM_015649 PE=3 SV=1[more]
tr|B9GK77|B9GK77_POPTR2.1e-8174.77Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_001G084000v3 PE=... [more]
tr|V4SX16|V4SX16_9ROSI3.6e-8173.85Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10020136mg PE=3 ... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0030170pyridoxal phosphate binding
Vocabulary: INTERPRO
TermDefinition
IPR015424PyrdxlP-dep_Trfase
IPR015421PyrdxlP-dep_Trfase_major
IPR000277Cys/Met-Metab_PyrdxlP-dep_enz
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009970 cellular response to sulfate starvation
biological_process GO:0042631 cellular response to water deprivation
biological_process GO:0019343 cysteine biosynthetic process via cystathionine
biological_process GO:0071266 'de novo' L-methionine biosynthetic process
biological_process GO:0051289 protein homotetramerization
biological_process GO:0019346 transsulfuration
cellular_component GO:0005829 cytosol
molecular_function GO:0004123 cystathionine gamma-lyase activity
molecular_function GO:0003962 cystathionine gamma-synthase activity
molecular_function GO:0018826 methionine gamma-lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G001530.1CsGy5G001530.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePFAMPF01053Cys_Met_Meta_PPcoord: 19..126
e-value: 1.2E-23
score: 83.1
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePANTHERPTHR11808TRANS-SULFURATION ENZYME FAMILY MEMBERcoord: 11..126
coord: 126..196
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3DG3DSA:3.40.640.10coord: 127..196
e-value: 1.8E-24
score: 88.1
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3DG3DSA:3.40.640.10coord: 7..126
e-value: 1.3E-26
score: 95.2
NoneNo IPR availablePANTHERPTHR11808:SF15CYSTATHIONINE GAMMA-LYASEcoord: 11..126
coord: 126..196
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILYSSF53383PLP-dependent transferasescoord: 35..196

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsGy5G001530Cucurbita maxima (Rimu)cgybcmaB608
CsGy5G001530Cucurbita maxima (Rimu)cgybcmaB638
CsGy5G001530Cucurbita moschata (Rifu)cgybcmoB583
CsGy5G001530Cucurbita moschata (Rifu)cgybcmoB617
CsGy5G001530Cucurbita pepo (Zucchini)cgybcpeB580
CsGy5G001530Cucurbita pepo (Zucchini)cgybcpeB685
CsGy5G001530Cucumber (Chinese Long) v2cgybcuB224
CsGy5G001530Bottle gourd (USVL1VR-Ls)cgyblsiB294
CsGy5G001530Melon (DHL92) v3.5.1cgybmeB298
CsGy5G001530Melon (DHL92) v3.6.1cgybmedB298
CsGy5G001530Watermelon (Charleston Gray)cgybwcgB332
CsGy5G001530Watermelon (97103) v1cgybwmB381
CsGy5G001530Wild cucumber (PI 183967)cgybcpiB226
CsGy5G001530Silver-seed gourdcarcgybB0370
CsGy5G001530Silver-seed gourdcarcgybB0709
CsGy5G001530Cucumber (Chinese Long) v3cgybcucB232
CsGy5G001530Watermelon (97103) v2cgybwmbB319
CsGy5G001530Wax gourdcgybwgoB413