CsGy4G000300 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy4G000300
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	Trypsin inhibitor 1
Location	Chr4 : 199923 .. 200165 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGAAAAAAGTTGCTTTAGTTGCAATGATGGGGATGTTGCTTATGGCTACTTTTACAGAGTCAGTCGGTTTGGGCGTGGATGAGGAGATCATTCAATTAGTTTCGGACGGGCTGAATGAGTACTCACAAAATATTGGAAAGGAAACTGTAGGCTGCCCTCGAATCCTTATGCCGTGTAAATCCGACCACGACTGCTTAAGTGGCTGCACTTGCAAACGAAACGGCTATTGTGGTTGA

mRNA sequence

ATGGAGTGGAAAAAAGTTGCTTTAGTTGCAATGATGGGGATGTTGCTTATGGCTACTTTTACAGAGTCAGTCGGTTTGGGCGTGGATGAGGAGATCATTCAATTAGTTTCGGACGGGCTGAATGAGTACTCACAAAATATTGGAAAGGAAACTGTAGGCTGCCCTCGAATCCTTATGCCGTGTAAATCCGACCACGACTGCTTAAGTGGCTGCACTTGCAAACGAAACGGCTATTGTGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAAAAAGTTGCTTTAGTTGCAATGATGGGGATGTTGCTTATGGCTACTTTTACAGAGTCAGTCGGTTTGGGCGTGGATGAGGAGATCATTCAATTAGTTTCGGACGGGCTGAATGAGTACTCACAAAATATTGGAAAGGAAACTGTAGGCTGCCCTCGAATCCTTATGCCGTGTAAATCCGACCACGACTGCTTAAGTGGCTGCACTTGCAAACGAAACGGCTATTGTGGTTGA

Protein sequence

MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNIGKETVGCPRILMPCKSDHDCLSGCTCKRNGYCG

Homology

[Close]

BLAST of CsGy4G000300 vs. NCBI nr
Match: KGN52761.1 (Trypsin inhibitor 1 [Cucumis sativus])

HSP 1 Score: 142.9 bits (359), Expect = 4.7e-31
Identity = 69/81 (85.19%), Postives = 73/81 (90.12%), Query Frame = 0


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNIGKETV-GCPRILM 60
          MEWKK+ALVAMMGMLLMATFTESVGLGVDEEIIQLVSDG+NEYSQNIGK+T  GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 PCKSDHDCLSGCTCKRNGYCG 81
           CK+D DC  GCTCK  GYCG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of CsGy4G000300 vs. NCBI nr
Match: XP_023552845.1 (uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 95.9 bits (237), Expect = 6.6e-17
Identity = 43/80 (53.75%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1   MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNIGKETVGCPRILMP 60
           MEWK+ AL+AM+G+LLMA F +S  +    E+IQLVSDG+N++ + +    +GCPRILM 
Sbjct: 85  MEWKRGALIAMVGLLLMAAFADSAAVH-GGEVIQLVSDGVNDFPRKMMNAALGCPRILME 144

Query: 61  CKSDHDCLSGCTCKRNGYCG 81
           C+ D DCL GC C+ NG+CG
Sbjct: 145 CEVDSDCLPGCVCRPNGFCG 163

[Close]

BLAST of CsGy4G000300 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-16
Identity = 44/80 (55.00%), Postives = 58/80 (72.50%), Query Frame = 0


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNIGKETVGCPRILMP 60
          MEWK+ AL+AM+G+LLMA F ES  +    E+IQLVSDG+N++ + +     GCPRILM 
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVH-GGEVIQLVSDGVNDFPRKMMNGVQGCPRILME 60

Query: 61 CKSDHDCLSGCTCKRNGYCG 81
          C+ D DCL GC C+ NG+CG
Sbjct: 61 CELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of CsGy4G000300 vs. NCBI nr
Match: Q43667.1 (RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inhibitor I; Flags: Precursor >CAA57704.1 Trichosanthes trypsin inhibitor-I [Trichosanthes kirilowii])

HSP 1 Score: 84.7 bits (208), Expect = 1.5e-13
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 17 MATFTESVGLGVD-EEIIQLVSDGLNEYSQNIGKETVGCPRILMPCKSDHDCLSGCTCKR 76
          MA F ES   G   +E+IQLVSDG+NEYS+ + +  V CPRILMPCK + DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGYCG 81
          NGYCG
Sbjct: 61 NGYCG 65

[Close]

BLAST of CsGy4G000300 vs. NCBI nr
Match: KGN52760.1 (hypothetical protein Csa_4G000805 [Cucumis sativus])

HSP 1 Score: 69.3 bits (168), Expect = 6.6e-09
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYS---QNIGKETVGCPRI 60
          MEWKK+ ++A++GML+MA   E     ++ E ++    GLN+     + + +ET  CPRI
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVK----GLNKNELPRKMMNEETRMCPRI 60

Query: 61 LMPCKSDHDCLSGCTCKRNGYCG 81
          L  C +D DC++ C C  NG+CG
Sbjct: 61 LEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CsGy4G000300 vs. Swiss-Prot
Match: sp|Q43667|ITR1_TRIKI (Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 5.0e-16
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 17 MATFTESVGLGVD-EEIIQLVSDGLNEYSQNIGKETVGCPRILMPCKSDHDCLSGCTCKR 76
          MA F ES   G   +E+IQLVSDG+NEYS+ + +  V CPRILMPCK + DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGYCG 81
          NGYCG
Sbjct: 61 NGYCG 65

[Close]

BLAST of CsGy4G000300 vs. Swiss-Prot
Match: sp|P34950|ITR5_LUFAE (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.8e-10
Identity = 33/66 (50.00%), Postives = 43/66 (65.15%), Query Frame = 0


Query: 17 MATFTESVGLGVDEEIIQLVSDGLNEYSQNI--GKETVGCPRILMPCKSDHDCLSGCTCK 76
          MA+  ES G+    E+I+L+SDG N+  + I  G+    CPRILMPCK+D DC+  C C 
Sbjct: 1  MASVAESSGV---VEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCL 60

Query: 77 RNGYCG 81
           NGYCG
Sbjct: 61 SNGYCG 63

[Close]

BLAST of CsGy4G000300 vs. Swiss-Prot
Match: sp|P11968|ITR2_BRYDI (Trypsin inhibitor 2 OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 8.5e-08
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 0


Query: 53 GCPRILMPCKSDHDCLSGCTCKRNGYCG 81
          GCPRILM CK D DCL+GC C++NGYCG
Sbjct: 2  GCPRILMRCKRDSDCLAGCVCQKNGYCG 29

[Close]

BLAST of CsGy4G000300 vs. Swiss-Prot
Match: sp|P12071|ITR2_ECBEL (Trypsin inhibitor 2 OS=Ecballium elaterium OX=3679 PE=1 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 1.2e-06
Identity = 20/28 (71.43%), Postives = 22/28 (78.57%), Query Frame = 0


Query: 53 GCPRILMPCKSDHDCLSGCTCKRNGYCG 81
          GCPRILM CK D DCL+GC C  NG+CG
Sbjct: 1  GCPRILMRCKQDSDCLAGCVCGPNGFCG 28

[Close]

BLAST of CsGy4G000300 vs. Swiss-Prot
Match: sp|P17680|ITR1_MOMRE (Trypsin inhibitor 1 OS=Momordica repens OX=3675 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.1e-06
Identity = 20/27 (74.07%), Postives = 21/27 (77.78%), Query Frame = 0


Query: 54 CPRILMPCKSDHDCLSGCTCKRNGYCG 81
          CPRILM CK D DCL+ C CKR GYCG
Sbjct: 3  CPRILMECKRDSDCLAQCVCKRQGYCG 29

[Close]

BLAST of CsGy4G000300 vs. TrEMBL
Match: tr|A0A0A0KY34|A0A0A0KY34_CUCSA (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.1e-31
Identity = 69/81 (85.19%), Postives = 73/81 (90.12%), Query Frame = 0


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNIGKETV-GCPRILM 60
          MEWKK+ALVAMMGMLLMATFTESVGLGVDEEIIQLVSDG+NEYSQNIGK+T  GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 PCKSDHDCLSGCTCKRNGYCG 81
           CK+D DC  GCTCK  GYCG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of CsGy4G000300 vs. TrEMBL
Match: tr|A0A0A0KT01|A0A0A0KT01_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.4e-09
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYS---QNIGKETVGCPRI 60
          MEWKK+ ++A++GML+MA   E     ++ E ++    GLN+     + + +ET  CPRI
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVK----GLNKNELPRKMMNEETRMCPRI 60

Query: 61 LMPCKSDHDCLSGCTCKRNGYCG 81
          L  C +D DC++ C C  NG+CG
Sbjct: 61 LEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CsGy4G000300 vs. TrEMBL
Match: tr|Q9S8D2|Q9S8D2_CUCME (CMETI-B=TRYPSIN inhibitor OS=Cucumis melo OX=3656 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 8.3e-08
Identity = 25/29 (86.21%), Postives = 27/29 (93.10%), Query Frame = 0


Query: 52 VGCPRILMPCKSDHDCLSGCTCKRNGYCG 81
          VGCPRILM CK+D DCL+GCTCKRNGYCG
Sbjct: 1  VGCPRILMKCKTDRDCLTGCTCKRNGYCG 29

[Close]

BLAST of CsGy4G000300 vs. TrEMBL
Match: tr|J7IN40|J7IN40_9ROSI (Two inhibitor peptide topologies 2 OS=Momordica sphaeroidea OX=703389 GN=TIPTOP2 PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 7.8e-06
Identity = 34/80 (42.50%), Postives = 45/80 (56.25%), Query Frame = 0


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNIGKETVGCPRILMP 60
          ME KK+  V ++ M+L+AT   S G   D + I L+SDG  +   N G     CP+IL  
Sbjct: 1  MESKKILPVVLVAMMLVAT---STGFN-DGDTIDLISDGRAQIDINGGI----CPKILQR 60

Query: 61 CKSDHDCLSGCTCKRNGYCG 81
          C+ D DC   C C+ NGYCG
Sbjct: 61 CRRDSDCPGACICRGNGYCG 72

[Close]

BLAST of CsGy4G000300 vs. TrEMBL
Match: tr|A0A0A7HIA9|A0A0A7HIA9_9ROSI (TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 8.6e-05
Identity = 29/77 (37.66%), Postives = 43/77 (55.84%), Query Frame = 0


Query: 10 AMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNI------GKETVGCPRILMPCKS 69
          A++ M+L+AT  +S G     + I L+S+G  +  Q+I       +E   CP+IL  C+ 
Sbjct: 3  ALVAMMLVATSADSNG----GDTIHLISNGRAQTGQDINSGGVYSEEQRACPKILKRCRR 62

Query: 70 DHDCLSGCTCKRNGYCG 81
          D DC   C C+ NGYCG
Sbjct: 63 DSDCPGACVCQDNGYCG 75

The following BLAST results are available for this feature:

BLAST of CsGy4G000300 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN52761.1	4.7e-31	85.19	Trypsin inhibitor 1 [Cucumis sativus]	[more]
XP_023552845.1	6.6e-17	53.75	uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo]	[more]
XP_022984819.1	1.1e-16	55.00	trypsin inhibitor 1-like [Cucurbita maxima]	[more]
Q43667.1	1.5e-13	60.00	RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inh...	[more]
KGN52760.1	6.6e-09	39.76	hypothetical protein Csa_4G000805 [Cucumis sativus]	[more]

BLAST of CsGy4G000300 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CsGy4G000300 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q43667\|ITR1_TRIKI	5.0e-16	60.00	Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1	[more]
sp\|P34950\|ITR5_LUFAE	1.8e-10	50.00	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
sp\|P11968\|ITR2_BRYDI	8.5e-08	75.00	Trypsin inhibitor 2 OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
sp\|P12071\|ITR2_ECBEL	1.2e-06	71.43	Trypsin inhibitor 2 OS=Ecballium elaterium OX=3679 PE=1 SV=2	[more]
sp\|P17680\|ITR1_MOMRE	2.1e-06	74.07	Trypsin inhibitor 1 OS=Momordica repens OX=3675 PE=1 SV=1	[more]

BLAST of CsGy4G000300 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KY34\|A0A0A0KY34_CUCSA	3.1e-31	85.19	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]
tr\|A0A0A0KT01\|A0A0A0KT01_CUCSA	4.4e-09	39.76	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1	[more]
tr\|Q9S8D2\|Q9S8D2_CUCME	8.3e-08	86.21	CMETI-B=TRYPSIN inhibitor OS=Cucumis melo OX=3656 PE=3 SV=1	[more]
tr\|J7IN40\|J7IN40_9ROSI	7.8e-06	42.50	Two inhibitor peptide topologies 2 OS=Momordica sphaeroidea OX=703389 GN=TIPTOP2...	[more]
tr\|A0A0A7HIA9\|A0A0A7HIA9_9ROSI	8.6e-05	37.66	TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011052	Proteinase_amylase_inhib_sf
IPR000737	Prot_inh_squash

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy4G000300.1	CsGy4G000300.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 52..80 e-value: 1.9E-9 score: 47.4
None	No IPR available	GENE3D	G3DSA:3.10.450.30		coord: 23..80 e-value: 5.1E-12 score: 47.9
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 54..80 e-value: 2.6E-13 score: 49.6
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 54..73
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 54..80 e-value: 3.60691E-10 score: 49.2983
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	SSF57027	Plant inhibitors of proteinases and amylases	coord: 50..80

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy4G000300	Cucumber (Chinese Long) v3	cgybcucB193
CsGy4G000300	Cucumber (Chinese Long) v3	cgybcucB171
CsGy4G000300	Cucumber (Chinese Long) v3	cgybcucB177
CsGy4G000300	Watermelon (97103) v2	cgybwmbB259
CsGy4G000300	Wax gourd	cgybwgoB342
CsGy4G000300	Cucumber (Gy14) v2	cgybcgybB122
CsGy4G000300	Cucurbita maxima (Rimu)	cgybcmaB494
CsGy4G000300	Cucurbita maxima (Rimu)	cgybcmaB543
CsGy4G000300	Cucurbita moschata (Rifu)	cgybcmoB465
CsGy4G000300	Cucurbita moschata (Rifu)	cgybcmoB517
CsGy4G000300	Cucurbita pepo (Zucchini)	cgybcpeB485
CsGy4G000300	Cucurbita pepo (Zucchini)	cgybcpeB504
CsGy4G000300	Cucumber (Chinese Long) v2	cgybcuB165
CsGy4G000300	Cucumber (Chinese Long) v2	cgybcuB177
CsGy4G000300	Bottle gourd (USVL1VR-Ls)	cgyblsiB257
CsGy4G000300	Melon (DHL92) v3.5.1	cgybmeB267
CsGy4G000300	Melon (DHL92) v3.5.1	cgybmeB269
CsGy4G000300	Melon (DHL92) v3.6.1	cgybmedB266
CsGy4G000300	Watermelon (Charleston Gray)	cgybwcgB251
CsGy4G000300	Watermelon (97103) v1	cgybwmB269
CsGy4G000300	Wild cucumber (PI 183967)	cgybcpiB168
CsGy4G000300	Wild cucumber (PI 183967)	cgybcpiB178
CsGy4G000300	Silver-seed gourd	carcgybB0309
CsGy4G000300	Silver-seed gourd	carcgybB0383