CsGy3G021070 (gene) Cucumber (Gy14) v2

Overview
NameCsGy3G021070
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionATP-dependent DNA helicase
LocationChr3 : 18924310 .. 18925122 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAGGGGGCCGAACAACACATTCTAGATTTAATATACCATTAGAAACACATGAGTCATCTATATGCAATATTTCTAAGCAAAGTAACAAGACAGAATTGATCAAGCAAGCAAAAGTTATAATTTGGGATGAGGTGCCAATGGCAAAAAAGGCCACAATCAAAGCAGTTGACAGAACTTTTAGAGATATAATGGATAATCCATTACCATTCGGTGGAAAAGTGGTTGTGTTAGGAGGAGATTTTCGACAAGTGTTACCTGTTGTCCCACGAGCTACAAGACAACAAACTATTAATGAAAGTTTGGTAAAGTCGTACCTTTGGAATAAAATGGAGAAAATCCAACTAACTAAAAATATGAGAGCTCAAAGTGATACCCGTTTTGCAGAATATTTGTTGAAGATTGGAAACGGAACTGAACTGTCAACAAAAGATGATTATATTTGTCTCCCTGCCAATATCATTGTTACAGGTGCAAATGAAGAAGATTCAATATTGAAGTTATTGAATATTGTTTATCCTGATCTCAAAACGCATACTACTTCAGCAGAATACATGACCAATAGAGCAATTCTATCAACAACAAATGAGTATGTTGACCAAATCAATGAAAGAATGATTGAGCTGTTTCCTGGAAAAATGGAAGAATTCATGAGTTTTGATGAAGCAATTGATGATACACATAATTATTATCATGAGGAATTTCTCAACTCATTGTTACCTAATGGTGTTCCTCCACACAAATTGTTTATTAAAAAAGATTGTCCAGTTATACTATTGAGAAACTTAGACCCCAGGCAATGGTCTATGTAA

mRNA sequence

ATGCCAGGGGGCCGAACAACACATTCTAGATTTAATATACCATTAGAAACACATGAGTCATCTATATGCAATATTTCTAAGCAAAGTAACAAGACAGAATTGATCAAGCAAGCAAAAGTTATAATTTGGGATGAGGTGCCAATGGCAAAAAAGGCCACAATCAAAGCAGTTGACAGAACTTTTAGAGATATAATGGATAATCCATTACCATTCGGTGGAAAAGTGGTTGTGTTAGGAGGAGATTTTCGACAAGTGTTACCTGTTGTCCCACGAGCTACAAGACAACAAACTATTAATGAAAGTTTGGTAAAGTCGTACCTTTGGAATAAAATGGAGAAAATCCAACTAACTAAAAATATGAGAGCTCAAAGTGATACCCGTTTTGCAGAATATTTGTTGAAGATTGGAAACGGAACTGAACTGTCAACAAAAGATGATTATATTTGTCTCCCTGCCAATATCATTGTTACAGGTGCAAATGAAGAAGATTCAATATTGAAGTTATTGAATATTGTTTATCCTGATCTCAAAACGCATACTACTTCAGCAGAATACATGACCAATAGAGCAATTCTATCAACAACAAATGAGTATGTTGACCAAATCAATGAAAGAATGATTGAGCTGTTTCCTGGAAAAATGGAAGAATTCATGAGTTTTGATGAAGCAATTGATGATACACATAATTATTATCATGAGGAATTTCTCAACTCATTGTTACCTAATGGTGTTCCTCCACACAAATTGTTTATTAAAAAAGATTGTCCAGTTATACTATTGAGAAACTTAGACCCCAGGCAATGGTCTATGTAA

Coding sequence (CDS)

ATGCCAGGGGGCCGAACAACACATTCTAGATTTAATATACCATTAGAAACACATGAGTCATCTATATGCAATATTTCTAAGCAAAGTAACAAGACAGAATTGATCAAGCAAGCAAAAGTTATAATTTGGGATGAGGTGCCAATGGCAAAAAAGGCCACAATCAAAGCAGTTGACAGAACTTTTAGAGATATAATGGATAATCCATTACCATTCGGTGGAAAAGTGGTTGTGTTAGGAGGAGATTTTCGACAAGTGTTACCTGTTGTCCCACGAGCTACAAGACAACAAACTATTAATGAAAGTTTGGTAAAGTCGTACCTTTGGAATAAAATGGAGAAAATCCAACTAACTAAAAATATGAGAGCTCAAAGTGATACCCGTTTTGCAGAATATTTGTTGAAGATTGGAAACGGAACTGAACTGTCAACAAAAGATGATTATATTTGTCTCCCTGCCAATATCATTGTTACAGGTGCAAATGAAGAAGATTCAATATTGAAGTTATTGAATATTGTTTATCCTGATCTCAAAACGCATACTACTTCAGCAGAATACATGACCAATAGAGCAATTCTATCAACAACAAATGAGTATGTTGACCAAATCAATGAAAGAATGATTGAGCTGTTTCCTGGAAAAATGGAAGAATTCATGAGTTTTGATGAAGCAATTGATGATACACATAATTATTATCATGAGGAATTTCTCAACTCATTGTTACCTAATGGTGTTCCTCCACACAAATTGTTTATTAAAAAAGATTGTCCAGTTATACTATTGAGAAACTTAGACCCCAGGCAATGGTCTATGTAA

Protein sequence

MPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRTFRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNMRAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHTTSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLLPNGVPPHKLFIKKDCPVILLRNLDPRQWSM
Homology
[Close]
BLAST of CsGy3G021070 vs. NCBI nr
Match: XP_022155233.1 (ATP-dependent DNA helicase PIF1-like [Momordica charantia])

HSP 1 Score: 414.8 bits (1065), Expect = 2.2e-112
Identity = 198/265 (74.72%), Postives = 230/265 (86.79%), Query Frame = 0 

Query: 1   MPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRT 60
           MPGGRT HSRFNIPL+ +ESS+CNISKQSN+ EL++QAK+IIWDE P AKK TI+AVDRT
Sbjct: 16  MPGGRTAHSRFNIPLQANESSMCNISKQSNRAELVRQAKLIIWDEAPXAKKVTIEAVDRT 75

Query: 61  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNM 120
           F+DIM+N  PFGGKV+V GGDFRQVLPVVPRATRQQT++ESLVKSYLW +MEK+QL KNM
Sbjct: 76  FQDIMENSKPFGGKVIVFGGDFRQVLPVVPRATRQQTVHESLVKSYLWKQMEKLQLKKNM 135

Query: 121 RAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHT 180
           RAQ+D +FAEYLLKIGNGTE S  +DY+ LP +I +     EDS+LKLLNIVYPDL  + 
Sbjct: 136 RAQNDYQFAEYLLKIGNGTEPSIVEDYVRLPKDIAIIDEGHEDSVLKLLNIVYPDLPQNA 195

Query: 181 TSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLL 240
            SAEY+TNRAILSTTNEYVD+IN +MI LF G +EEFMSFDE IDDTH+YY EEFLNSLL
Sbjct: 196 MSAEYITNRAILSTTNEYVDEINNKMIRLFLGNIEEFMSFDETIDDTHSYYQEEFLNSLL 255

Query: 241 PNGVPPHKLFIKKDCPVILLRNLDP 266
           PNG+PPHKL +K +CP+ILLRNLDP
Sbjct: 256 PNGIPPHKLILKINCPIILLRNLDP 280

[Close]
BLAST of CsGy3G021070 vs. NCBI nr
Match: XP_022152307.1 (ATP-dependent DNA helicase PIF1-like [Momordica charantia])

HSP 1 Score: 365.2 bits (936), Expect = 2.0e-97
Identity = 175/218 (80.28%), Postives = 197/218 (90.37%), Query Frame = 0 

Query: 48  MAKKATIKAVDRTFRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYL 107
           MAKK TI+AVDRTFRDIMD+  PFGGK+VV GGDFRQVLPVVPRATRQQTINESLVKSYL
Sbjct: 1   MAKKTTIEAVDRTFRDIMDSTEPFGGKIVVFGGDFRQVLPVVPRATRQQTINESLVKSYL 60

Query: 108 WNKMEKIQLTKNMRAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILK 167
           W +MEK+QLTKNMRA SD++FAEYLLKIGNGTE S +DDYICL  +I +TG N+E +I+K
Sbjct: 61  WKQMEKLQLTKNMRAHSDSQFAEYLLKIGNGTEPSVEDDYICLTDDITITGENDEGAIMK 120

Query: 168 LLNIVYPDLKTHTTSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDT 227
           LLN+VYPDL+ + TS+EYMTNR ILSTTNEYVDQINE+MI+LFPGK  EFMSFDEAIDDT
Sbjct: 121 LLNVVYPDLEQNATSSEYMTNRVILSTTNEYVDQINEKMIQLFPGKFFEFMSFDEAIDDT 180

Query: 228 HNYYHEEFLNSLLPNGVPPHKLFIKKDCPVILLRNLDP 266
           HNYY EEFLNSLLPNG+PPHKL +K +CPVILLRNLDP
Sbjct: 181 HNYYQEEFLNSLLPNGIPPHKLMLKANCPVILLRNLDP 218

[Close]
BLAST of CsGy3G021070 vs. NCBI nr
Match: XP_020242961.1 (uncharacterized protein LOC109821182 [Asparagus officinalis])

HSP 1 Score: 337.4 bits (864), Expect = 4.4e-89
Identity = 158/265 (59.62%), Postives = 210/265 (79.25%), Query Frame = 0 

Query: 1   MPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRT 60
           MPGGRT HSRF IP+  +E+++CNISKQ   ++LI+ A +IIWDE PMAK+  I+ VDRT
Sbjct: 596 MPGGRTAHSRFKIPITANETTMCNISKQDGTSKLIRLASLIIWDEAPMAKRFAIETVDRT 655

Query: 61  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNM 120
            RD+M +   FGGKVVV GGDFRQVLPVVPR TR +T+N SLVKSYLW KMEK+ LT NM
Sbjct: 656 LRDVMGDKRIFGGKVVVFGGDFRQVLPVVPRGTRAETVNASLVKSYLWEKMEKLSLTINM 715

Query: 121 RAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHT 180
           RA++D  F E+LL++GNG E +  ++ I LP  ++V   N+E+S   L++ ++P L++++
Sbjct: 716 RARTDQVFGEFLLRVGNGEEPTIDENLILLPHEMVVEYDNDENSEKNLIDAIFPSLQSNS 775

Query: 181 TSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLL 240
           +SAEYMT RAILST NEYVD +NE++I LFPG+ + F SF EA+DDT+NYY EEFLN+L+
Sbjct: 776 SSAEYMTQRAILSTRNEYVDGLNEKLIALFPGEAKTFFSFVEAVDDTNNYYQEEFLNTLM 835

Query: 241 PNGVPPHKLFIKKDCPVILLRNLDP 266
           PNG+PPHKL +K++C ++LLRNLDP
Sbjct: 836 PNGLPPHKLILKRNCLIMLLRNLDP 860

[Close]
BLAST of CsGy3G021070 vs. NCBI nr
Match: XP_020243613.1 (ATP-dependent DNA helicase PIF1-like [Asparagus officinalis])

HSP 1 Score: 334.0 bits (855), Expect = 4.9e-88
Identity = 158/265 (59.62%), Postives = 207/265 (78.11%), Query Frame = 0 

Query: 1   MPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRT 60
           MPGGRT HSRF I + T +SS+C ISKQ   ++L++ A++IIWDE  MAK+  I+ +DRT
Sbjct: 16  MPGGRTAHSRFKIKIPTTDSSMCGISKQDGTSKLLRIARLIIWDEAAMAKRVAIETLDRT 75

Query: 61  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNM 120
            RD+MD   PFGGKV+V GGDFRQVLPVVPR+TR QTI ESLV S+LW KM K+QL+KNM
Sbjct: 76  LRDLMDKDEPFGGKVIVFGGDFRQVLPVVPRSTRSQTIKESLVSSHLWRKMIKLQLSKNM 135

Query: 121 RAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHT 180
           RA+SDT F+E+LL+IGNG E +  +D I LP  +++    +E     L+N ++P L  + 
Sbjct: 136 RAKSDTAFSEFLLRIGNGDEPTVTEDLIKLPTAMLIKNQGDELPEDSLINSIFPSLAENF 195

Query: 181 TSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLL 240
           TS +YM +RAIL+T NEYVD +N+R+I+ FPG+ +EF SFDEA+DDT+++Y EEFLN+LL
Sbjct: 196 TSIDYMKDRAILATKNEYVDMLNDRLIKRFPGEGKEFYSFDEAVDDTNHHYQEEFLNTLL 255

Query: 241 PNGVPPHKLFIKKDCPVILLRNLDP 266
           PNG+PPHKL +KK CP+ILLRNLDP
Sbjct: 256 PNGLPPHKLLLKKYCPIILLRNLDP 280

[Close]
BLAST of CsGy3G021070 vs. NCBI nr
Match: XP_020262212.1 (ATP-dependent DNA helicase PIF7-like [Asparagus officinalis])

HSP 1 Score: 333.2 bits (853), Expect = 8.3e-88
Identity = 156/265 (58.87%), Postives = 208/265 (78.49%), Query Frame = 0 

Query: 1   MPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRT 60
           MPGGRT HSRF IP+  +E+ +CNISKQ   ++LI+ A +IIWDE PMAK+  I+ VDRT
Sbjct: 329 MPGGRTAHSRFKIPITANETIMCNISKQDGTSKLIRLASLIIWDEAPMAKRFAIETVDRT 388

Query: 61  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNM 120
            RD+M +   FGGKVVV GGDFRQVLPVVPR TR +T+N SLVKSYLW KMEK+ LT NM
Sbjct: 389 LRDVMGDKRIFGGKVVVFGGDFRQVLPVVPRGTRAETVNASLVKSYLWEKMEKLSLTINM 448

Query: 121 RAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHT 180
           RA++D  F E+LL++GNG E +  ++ I LP  ++V   N+E+S   L++ ++P L++++
Sbjct: 449 RARTDQVFGEFLLRVGNGEEPTIDENLILLPHEMVVEYDNDENSEKNLIDAIFPSLQSNS 508

Query: 181 TSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLL 240
           +SAEYMT RAILST NEYVD +NE++I LF G+ + F SFDEA+DDT+NY+ EEFLN+ +
Sbjct: 509 SSAEYMTQRAILSTRNEYVDGLNEKLIALFLGEAKTFFSFDEAVDDTNNYHQEEFLNTFM 568

Query: 241 PNGVPPHKLFIKKDCPVILLRNLDP 266
           PNG+ PHKL +K++CP++LLRNLDP
Sbjct: 569 PNGLQPHKLILKRNCPIMLLRNLDP 593

[Close]
BLAST of CsGy3G021070 vs. TAIR10
Match: AT3G51690.1 (PIF1 helicase)

HSP 1 Score: 62.4 bits (150), Expect = 5.0e-10
Identity = 44/146 (30.14%), Postives = 79/146 (54.11%), Query Frame = 0 

Query: 125 DTRFAEYLLKIGNGTELSTKDD---YICLPANIIVTGANEEDSILKLLNIVYPDLKTHTT 184
           D   + +++ I +G  ++  +D    I +  ++++T    +D I  LL  VY +    + 
Sbjct: 56  DEALSRWIMNI-SGDNINNPNDGETEIDISKDLLIT--ESKDPIKTLLKEVYGEYFAKSY 115

Query: 185 SAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHN--YYHEEFLNSL 244
           + ++  + AIL   ++ VDQIN+ M+ L PG+ +E +S D      ++  +   E LNS+
Sbjct: 116 NPDFCHDSAILCHRDDDVDQINDYMLSLLPGEEKECLSTDSISPSPNDDMFVPLEVLNSI 175

Query: 245 LPNGVPPHKLFIKKDCPVILLRNLDP 266
              G+P  KL +K   PV+LLR+LDP
Sbjct: 176 KVPGLPDFKLRLKVGAPVMLLRDLDP 198

[Close]
BLAST of CsGy3G021070 vs. TAIR10
Match: AT3G51700.1 (PIF1 helicase)

HSP 1 Score: 61.2 bits (147), Expect = 1.1e-09
Identity = 45/152 (29.61%), Postives = 76/152 (50.00%), Query Frame = 0 

Query: 125 DTRFAEYLLKIGNGTELSTKDD---YICLPANIIVTGANEEDSILKLLNIVYPDLKTHTT 184
           D  F +++  IG G  ++  +D    I +  ++++T    +D I  +++ VY +  T + 
Sbjct: 57  DEAFTKWITNIG-GENINKPNDGETKIDIHEDLLITEC--KDPIKTIVDEVYGESFTESY 116

Query: 185 SAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFD-----EAIDDTHNYYHEEFL 244
           + ++   RAIL  TN+  D+IN+ M+    G+  +    D      A  +    Y  EFL
Sbjct: 117 NPDFYQERAILCHTNDVADEINDYMLSQLQGEETKCYGADTIYPTHASPNDKMLYPLEFL 176

Query: 245 NSLLPNGVPPHKLFIKKDCPVILLRNLDPRQW 269
           NS+   G P  KL +K   PV+LLR+L P  W
Sbjct: 177 NSIKIPGFPDFKLRLKVGAPVMLLRDLAPYGW 205

[Close]
BLAST of CsGy3G021070 vs. TAIR10
Match: AT5G28780.1 (PIF1 helicase)

HSP 1 Score: 60.8 bits (146), Expect = 1.4e-09
Identity = 33/101 (32.67%), Postives = 53/101 (52.48%), Query Frame = 0 

Query: 173 YPDLKTHTTSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMS------FDEAIDD 232
           Y        + +Y+T R IL+  NEYVD+IN  M+    G  +E++S       D    D
Sbjct: 102 YVQFSVSQPNFQYLTERGILTPHNEYVDEINAYMLSQVGGDSKEYLSSYSIGKADTIGAD 161

Query: 233 THNYYHEEFLNSLLPNGVPPHKLFIKKDCPVILLRNLDPRQ 268
               YH ++LNSL    +P HK+ +KK  P++ +RN + ++
Sbjct: 162 YEALYHVKYLNSLEFPSLPKHKISLKKGVPIMQMRNFNQKE 202

[Close]
BLAST of CsGy3G021070 vs. Swiss-Prot
Match: sp|A6ZM04|PIF1_YEAS7 (ATP-dependent DNA helicase PIF1 OS=Saccharomyces cerevisiae (strain YJM789) OX=307796 GN=PIF1 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.9e-07
Identity = 69/267 (25.84%), Postives = 108/267 (40.45%), Query Frame = 0 

Query: 3   GGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKV--IIWDEVPMAKKATIKAVDRT 62
           GG T HS   I L   ++       + ++  L +   +  ++ DE+ M     +  +D  
Sbjct: 298 GGITIHSFAGIGLGKGDADKLYKKVRRSRKHLRRWENIGALVVDEISMLDAELLDKLDFI 357

Query: 63  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEK--IQLTK 122
            R I  N  PFGG  ++  GDF Q+ PV     R     +   +S  W +  K  I L K
Sbjct: 358 ARKIRKNHQPFGGIQLIFCGDFFQLPPVSKDPNRP---TKFAFESKAWKEGVKMTIMLQK 417

Query: 123 NMRAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKT 182
             R + D +F + L    N   L   DD              E +   K L+   PD   
Sbjct: 418 VFRQRGDVKFIDML----NRMRLGNIDD--------------ETEREFKKLSRPLPD--D 477

Query: 183 HTTSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFD-EAIDDTHNYYHEEFLN 242
               AE       L +T   V++ N   +   PG++  F + D  A++D      EE   
Sbjct: 478 EIIPAE-------LYSTRMEVERANNSRLSKLPGQVHIFNAIDGGALED------EELKE 528

Query: 243 SLLPNGVPPHKLFIKKDCPVILLRNLD 265
            LL N + P +L +K    V++++NLD
Sbjct: 538 RLLQNFLAPKELHLKVGAQVMMVKNLD 528

[Close]
BLAST of CsGy3G021070 vs. Swiss-Prot
Match: sp|P07271|PIF1_YEAST (ATP-dependent DNA helicase PIF1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PIF1 PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 4.9e-07
Identity = 69/267 (25.84%), Postives = 108/267 (40.45%), Query Frame = 0 

Query: 3   GGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKV--IIWDEVPMAKKATIKAVDRT 62
           GG T HS   I L   ++       + ++  L +   +  ++ DE+ M     +  +D  
Sbjct: 298 GGITIHSFAGIGLGKGDADKLYKKVRRSRKHLRRWENIGALVVDEISMLDAELLDKLDFI 357

Query: 63  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEK--IQLTK 122
            R I  N  PFGG  ++  GDF Q+ PV     R     +   +S  W +  K  I L K
Sbjct: 358 ARKIRKNHQPFGGIQLIFCGDFFQLPPVSKDPNRP---TKFAFESKAWKEGVKMTIMLQK 417

Query: 123 NMRAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKT 182
             R + D +F + L    N   L   DD              E +   K L+   PD   
Sbjct: 418 VFRQRGDVKFIDML----NRMRLGNIDD--------------ETEREFKKLSRPLPD--D 477

Query: 183 HTTSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFD-EAIDDTHNYYHEEFLN 242
               AE       L +T   V++ N   +   PG++  F + D  A++D      EE   
Sbjct: 478 EIIPAE-------LYSTRMEVERANNSRLSKLPGQVHIFNAIDGGALED------EELKE 528

Query: 243 SLLPNGVPPHKLFIKKDCPVILLRNLD 265
            LL N + P +L +K    V++++NLD
Sbjct: 538 RLLQNFLAPKELHLKVGAQVMMVKNLD 528

[Close]
BLAST of CsGy3G021070 vs. Swiss-Prot
Match: sp|P38766|RRM3_YEAST (ATP-dependent DNA helicase RRM3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RRM3 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-06
Identity = 69/264 (26.14%), Postives = 110/264 (41.67%), Query Frame = 0 

Query: 3   GGRTTH--SRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRT 62
           GG T H  S   I  +T +  +  I  Q +     +  KV+I DE+ M     +  +++ 
Sbjct: 294 GGSTLHKWSGIGIGNKTIDQLVKKIQSQKDLLAAWRYTKVLIIDEISMVDGNLLDKLEQI 353

Query: 63  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNK--MEKIQLTK 122
            R I  N  PFGG  +VL GDF Q+ PV  +   +  + +   +S +W +   + I LTK
Sbjct: 354 ARRIRKNDDPFGGIQLVLTGDFFQLPPVAKK--DEHNVVKFCFESEMWKRCIQKTILLTK 413

Query: 123 NMRAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKT 182
             R Q D +  + L  I  G           L  +I  T  N         +I Y D   
Sbjct: 414 VFR-QQDNKLIDILNAIRYGE----------LTVDIAKTIRNLN------RDIDYADGIA 473

Query: 183 HTTSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNS 242
            T           L  T   V+  N + ++  PG + EF    +A+D+    Y     +S
Sbjct: 474 PTE----------LYATRREVELSNVKKLQSLPGDLYEF----KAVDNAPERYQAILDSS 520

Query: 243 LLPNGVPPHKLFIKKDCPVILLRN 263
           L+   V    + +K+D  V++L+N
Sbjct: 534 LMVEKV----VALKEDAQVMMLKN 520

[Close]
BLAST of CsGy3G021070 vs. Swiss-Prot
Match: sp|A5WFR0|PIF1_PSYWF (ATP-dependent DNA helicase pif1 OS=Psychrobacter sp. (strain PRwf-1) OX=349106 GN=pif1 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.9e-06
Identity = 57/216 (26.39%), Postives = 92/216 (42.59%), Query Frame = 0 

Query: 4   GRTTHSRFNIPL--ETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRTF 63
           G T HS   I +  E  E  + N+S++    + +++  V+I DE+ M     +  V++  
Sbjct: 59  GITIHSWSGIGIKDELTERDLVNLSRKKVLKDRLQETAVLIIDEISMLHAKQLNLVNQVL 118

Query: 64  RDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNMR 123
           + +  N  PFGG  VV+ GDF Q+ PV  R    +     + +++L    +   L++  R
Sbjct: 119 KHMRQNDKPFGGIQVVVAGDFFQLPPVGSRGESNRDKFAFMSQAWLDAGFKICYLSEQHR 178

Query: 124 AQSDTRFAEYLLKIGNGTELSTKDDYICLPANII--VTGAN--EEDSILKLLNIVYPDLK 183
            QS           G G +     D      NI+  + G N     +I  L N  Y D+ 
Sbjct: 179 QQS-----------GEGEDAQITLD------NILNQIRGENGVSAAAIAALQNTFYQDVD 238

Query: 184 THTTSAEYMTNRAILSTTNEYVDQINERMIELFPGK 214
                     NR  L T N  V++INE  + L  G+
Sbjct: 239 ---------VNRTRLYTHNVNVNKINEHELALLEGE 248

[Close]
BLAST of CsGy3G021070 vs. Swiss-Prot
Match: sp|Q9QSK3|VF030_IIV6 (Uncharacterized protein 030L OS=Invertebrate iridescent virus 6 OX=176652 GN=IIV6-030L PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 9.3e-06
Identity = 48/145 (33.10%), Postives = 62/145 (42.76%), Query Frame = 0 

Query: 3   GGRTTHSRFNIPL--ETHESSICNISKQSN--KTELIKQAKVIIWDEVPMAKKATIKAVD 62
           GG T HS   I L   T E  +  I K S   K  + K+   +I DEV M        ++
Sbjct: 70  GGSTLHSYLGIGLGDGTIEDLVQRIKKASKGIKERVWKELTTLIIDEVSMLNPVLFDKLE 129

Query: 63  RTFRDIMDNPLPFGGKVVVLGGDFRQVLPVVP---RATRQQTINESLVKSYLWNKM--EK 122
           +  R I  + LPFGG  ++L GD  Q LPVV       +     E +  +  W K     
Sbjct: 130 KIARIIRGSDLPFGGIQLILSGDLLQ-LPVVKGGGTGNKNDHNMEFVTDANSWKKCIGNN 189

Query: 123 IQLTKNMRAQSDTRFAEYLLKIGNG 139
           I L   +  Q D  F E LLKI  G
Sbjct: 190 IVLLTEIMRQKDFHFKEILLKIRVG 213

[Close]
BLAST of CsGy3G021070 vs. TrEMBL
Match: tr|A0A1U7V7Y5|A0A1U7V7Y5_NICSY (ATP-dependent DNA helicase OS=Nicotiana sylvestris OX=4096 GN=LOC104213305 PE=3 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 4.1e-83
Identity = 153/265 (57.74%), Postives = 205/265 (77.36%), Query Frame = 0 

Query: 1   MPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRT 60
           +PGGRTTHSRF+IPL+T +++I N+SKQS   +LI+++K+IIWDE PMA + TI+ VDR+
Sbjct: 141 LPGGRTTHSRFDIPLQTTDTTITNMSKQSGGAKLIRKSKLIIWDEAPMANRQTIETVDRS 200

Query: 61  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNM 120
           FRDIMD   PFGGKV+V GGDFRQVLPVVP++TR +TIN SLVKSYLWNKMEKIQL +NM
Sbjct: 201 FRDIMDINEPFGGKVMVFGGDFRQVLPVVPKSTRVETINASLVKSYLWNKMEKIQLIRNM 260

Query: 121 RAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHT 180
           RA++D  F+++LL+IGNG E + + D I L   +IV    +      L+  ++P L   +
Sbjct: 261 RARADPTFSDFLLRIGNGEEPTIRYDMILLLEKLIVKHDGDSIGEDNLIREIFPSLDEMS 320

Query: 181 TSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLL 240
           + A+YMT RAIL++ NEYVDQ+NE +I  FPG+   F+SFD A DDT+NY  EE+LN+L 
Sbjct: 321 SCAKYMTERAILASRNEYVDQLNEMLIAKFPGESRTFLSFDSAEDDTNNYCQEEYLNNLT 380

Query: 241 PNGVPPHKLFIKKDCPVILLRNLDP 266
           PNG+PPH++ +KK+ P++LLRNL P
Sbjct: 381 PNGLPPHRVVLKKNAPIMLLRNLYP 405

[Close]
BLAST of CsGy3G021070 vs. TrEMBL
Match: tr|A0A287JAN4|A0A287JAN4_HORVV (ATP-dependent DNA helicase OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.6e-82
Identity = 149/265 (56.23%), Postives = 199/265 (75.09%), Query Frame = 0 

Query: 1   MPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRT 60
           MPGGRT HSRF IP++   +S+C+ +KQS  TEL+KQA ++IWDEV M K+  ++ +DR+
Sbjct: 178 MPGGRTAHSRFKIPIKLTHNSMCSFTKQSGTTELLKQASLLIWDEVAMTKRQAVETLDRS 237

Query: 61  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNM 120
            +DIM+  LPFGGKVVV GGDFRQVLPVV R TR Q  + +L++SY+W K+ KI+LT+NM
Sbjct: 238 LQDIMECALPFGGKVVVFGGDFRQVLPVVTRGTRAQITDATLLRSYMWEKICKIRLTRNM 297

Query: 121 RAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHT 180
           RAQ+D  F+EYLL+IGNGTE +  DDY+CL  +I++T   +E  + KL+  V+P L T+ 
Sbjct: 298 RAQTDPWFSEYLLRIGNGTEKTIGDDYVCLREDIVITYTEDEKQVNKLIEDVFPSLHTNA 357

Query: 181 TSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLL 240
           TS EYM+ RAILST NE+VD +N++MI  FPG+ + + SFD   DD  N Y  +FLNS+ 
Sbjct: 358 TSREYMSTRAILSTKNEHVDDLNDKMISRFPGEEKVYHSFDSIEDDFQNNYTIDFLNSIT 417

Query: 241 PNGVPPHKLFIKKDCPVILLRNLDP 266
           PNG+PPH L +K +CP ILLRNLDP
Sbjct: 418 PNGLPPHVLKVKINCPAILLRNLDP 442

[Close]
BLAST of CsGy3G021070 vs. TrEMBL
Match: tr|A0A287ECJ4|A0A287ECJ4_HORVV (ATP-dependent DNA helicase OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.0e-82
Identity = 149/265 (56.23%), Postives = 200/265 (75.47%), Query Frame = 0 

Query: 1   MPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRT 60
           MPGGRT HSRF IP++  ++S+C+ +KQS   EL+KQA +IIWDEV M K+  ++ +DR+
Sbjct: 178 MPGGRTAHSRFKIPIKLTDNSMCSFTKQSGTAELLKQASLIIWDEVAMTKRQAVETLDRS 237

Query: 61  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNM 120
            +DIM  PLPFGGKVVV GGDFRQVLPVV R TR Q I+ +L++SYLW+K+ KI+LT+NM
Sbjct: 238 LQDIMGCPLPFGGKVVVFGGDFRQVLPVVTRGTRAQIIDATLLRSYLWDKIRKIRLTRNM 297

Query: 121 RAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHT 180
            AQ+D  F+EYLL+IGNGTE +  DDY+CLP +I++    +E ++ KL+  V+P L  + 
Sbjct: 298 WAQADPWFSEYLLRIGNGTEETIGDDYVCLPNDIVIAYTEDEKAMNKLIEHVFPSLHANA 357

Query: 181 TSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLL 240
            S EYM+ RAILST N++VD +N++MI  FPG+ + + SFD   DD  N Y  +FLNS+ 
Sbjct: 358 KSREYMSTRAILSTKNDHVDDLNDKMISRFPGEEKLYHSFDSIEDDMQNNYTIDFLNSIT 417

Query: 241 PNGVPPHKLFIKKDCPVILLRNLDP 266
           PNG+PPH L +K +CPVILLRNLDP
Sbjct: 418 PNGLPPHVLRLKVNCPVILLRNLDP 442

[Close]
BLAST of CsGy3G021070 vs. TrEMBL
Match: tr|A0A287ELL5|A0A287ELL5_HORVV (ATP-dependent DNA helicase OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.5e-82
Identity = 149/265 (56.23%), Postives = 198/265 (74.72%), Query Frame = 0 

Query: 1   MPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRT 60
           MPGGRT HSRF IP++   +S+C+ +KQS   EL+KQA ++IWDEV M K+  ++ +DR+
Sbjct: 128 MPGGRTAHSRFKIPIKLTHNSMCSFTKQSGTAELLKQASLLIWDEVAMTKRQAVETLDRS 187

Query: 61  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNM 120
            +DIM+  LPFGGKVVV GGDFRQVLPVV R TR Q  + +L++SYLW K+ KI+LT+NM
Sbjct: 188 LQDIMECALPFGGKVVVFGGDFRQVLPVVTRGTRAQITDATLLRSYLWEKICKIRLTRNM 247

Query: 121 RAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHT 180
           RAQ+D  F+EYLL+IGNG E +  DDY+CLP +I++T   +E  + KL+  V+P L T+ 
Sbjct: 248 RAQTDPWFSEYLLRIGNGMEKTIGDDYVCLPEDIVITYTEDEKQVNKLIEDVFPSLHTNA 307

Query: 181 TSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLL 240
           TS EYM+ RAILST NE+VD +N++MI  FPG+ + + SFD    D  N Y  +FLNS+ 
Sbjct: 308 TSREYMSTRAILSTKNEHVDDLNDKMISRFPGEEKVYNSFDSIEHDFQNNYTIDFLNSIT 367

Query: 241 PNGVPPHKLFIKKDCPVILLRNLDP 266
           PNG+PPH L +K +CPVILLRNLDP
Sbjct: 368 PNGLPPHVLKVKINCPVILLRNLDP 392

[Close]
BLAST of CsGy3G021070 vs. TrEMBL
Match: tr|A0A287XHR8|A0A287XHR8_HORVV (ATP-dependent DNA helicase OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.9e-80
Identity = 147/265 (55.47%), Postives = 198/265 (74.72%), Query Frame = 0 

Query: 1   MPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKATIKAVDRT 60
           MPGGRT HSRF IP++  ++S+C+ +KQS  TEL+KQA +IIWDEV M K+  ++ +DR+
Sbjct: 128 MPGGRTAHSRFKIPIKLTDNSMCSFTKQSGTTELLKQASLIIWDEVAMTKRQAVETLDRS 187

Query: 61  FRDIMDNPLPFGGKVVVLGGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNM 120
            +DIM  PLPFGGKVVV GGDFRQVLPVV R TR Q  + +L++SYLW K+ KI+LT+NM
Sbjct: 188 LQDIMGCPLPFGGKVVVFGGDFRQVLPVVTRGTRAQITDATLLRSYLWVKIRKIRLTRNM 247

Query: 121 RAQSDTRFAEYLLKIGNGTELSTKDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHT 180
           RAQ+D  F+EYLL+IGNGTE +  DDY+ LP +I+     +E ++ KL+  V+P L  + 
Sbjct: 248 RAQADPWFSEYLLRIGNGTEETIGDDYVRLPNDIVTAYTEDEKAMNKLIEDVFPSLHANA 307

Query: 181 TSAEYMTNRAILSTTNEYVDQINERMIELFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLL 240
            S EYM+ RAILST N++VD +N++MI  FPG+ + + SF+   DD  N Y  +FLNS+ 
Sbjct: 308 KSREYMSTRAILSTKNDHVDDLNDKMISRFPGEEKLYHSFNSIEDDMQNNYTIDFLNSIT 367

Query: 241 PNGVPPHKLFIKKDCPVILLRNLDP 266
           PNG+PPH L +K +CP+ILLRNLDP
Sbjct: 368 PNGLPPHVLRLKVNCPIILLRNLDP 392

The following BLAST results are available for this feature:
BLAST of CsGy3G021070 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022155233.12.2e-11274.72ATP-dependent DNA helicase PIF1-like [Momordica charantia][more]
XP_022152307.12.0e-9780.28ATP-dependent DNA helicase PIF1-like [Momordica charantia][more]
XP_020242961.14.4e-8959.62uncharacterized protein LOC109821182 [Asparagus officinalis][more]
XP_020243613.14.9e-8859.62ATP-dependent DNA helicase PIF1-like [Asparagus officinalis][more]
XP_020262212.18.3e-8858.87ATP-dependent DNA helicase PIF7-like [Asparagus officinalis][more]
BLAST of CsGy3G021070 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G51690.15.0e-1030.14PIF1 helicase[more]
AT3G51700.11.1e-0929.61PIF1 helicase[more]
AT5G28780.11.4e-0932.67PIF1 helicase[more]
BLAST of CsGy3G021070 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|A6ZM04|PIF1_YEAS74.9e-0725.84ATP-dependent DNA helicase PIF1 OS=Saccharomyces cerevisiae (strain YJM789) OX=3... [more]
sp|P07271|PIF1_YEAST4.9e-0725.84ATP-dependent DNA helicase PIF1 OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
sp|P38766|RRM3_YEAST1.4e-0626.14ATP-dependent DNA helicase RRM3 OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
sp|A5WFR0|PIF1_PSYWF1.9e-0626.39ATP-dependent DNA helicase pif1 OS=Psychrobacter sp. (strain PRwf-1) OX=349106 G... [more]
sp|Q9QSK3|VF030_IIV69.3e-0633.10Uncharacterized protein 030L OS=Invertebrate iridescent virus 6 OX=176652 GN=IIV... [more]
BLAST of CsGy3G021070 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1U7V7Y5|A0A1U7V7Y5_NICSY4.1e-8357.74ATP-dependent DNA helicase OS=Nicotiana sylvestris OX=4096 GN=LOC104213305 PE=3 ... [more]
tr|A0A287JAN4|A0A287JAN4_HORVV1.6e-8256.23ATP-dependent DNA helicase OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1[more]
tr|A0A287ECJ4|A0A287ECJ4_HORVV2.0e-8256.23ATP-dependent DNA helicase OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1[more]
tr|A0A287ELL5|A0A287ELL5_HORVV3.5e-8256.23ATP-dependent DNA helicase OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1[more]
tr|A0A287XHR8|A0A287XHR8_HORVV1.9e-8055.47ATP-dependent DNA helicase OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0000723telomere maintenance
GO:0006281DNA repair
Vocabulary: Molecular Function
TermDefinition
GO:0003678DNA helicase activity
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
IPR010285DNA_helicase_pif1-like
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0006281 DNA repair
biological_process GO:0000723 telomere maintenance
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
molecular_function GO:0003678 DNA helicase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G021070.1CsGy3G021070.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010285DNA helicase Pif1-likePFAMPF05970PIF1coord: 1..266
e-value: 2.0E-82
score: 277.2
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 2..119
e-value: 6.2E-13
score: 50.5
NoneNo IPR availablePANTHERPTHR10492UNCHARACTERIZEDcoord: 1..265
NoneNo IPR availablePANTHERPTHR10492:SF21SUBFAMILY NOT NAMEDcoord: 1..265
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 245..265
coord: 69..139
coord: 185..217