CsGy2G008620 (gene) Cucumber (Gy14) v2

Overview
NameCsGy2G008620
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
Description3-isopropylmalate dehydratase small subunit 3-like
LocationChr2 : 7708286 .. 7708504 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACCAAATATTTCATCATAATTATTGACGACAACTTCAGCTGTGGTTCTTCACGGGAGCACGCGTTGGTTGCGTTGGGAGCGTCCGACGTTGTGGCGATAGTGGATGAATCTTATGCACATATTTTCTTCACAGCAAACTCCGTGGCAACTAGAGAGATTTATCCATTGAAATCGGAGACTAGAATTTCTGCGAATTTTAAAATTAAAAAATAA

mRNA sequence

ATGAAAACCAAATATTTCATCATAATTATTGACGACAACTTCAGCTGTGGTTCTTCACGGGAGCACGCGTTGGTTGCGTTGGGAGCGTCCGACGTTGTGGCGATAGTGGATGAATCTTATGCACATATTTTCTTCACAGCAAACTCCGTGGCAACTAGAGAGATTTATCCATTGAAATCGGAGACTAGAATTTCTGCGAATTTTAAAATTAAAAAATAA

Coding sequence (CDS)

ATGAAAACCAAATATTTCATCATAATTATTGACGACAACTTCAGCTGTGGTTCTTCACGGGAGCACGCGTTGGTTGCGTTGGGAGCGTCCGACGTTGTGGCGATAGTGGATGAATCTTATGCACATATTTTCTTCACAGCAAACTCCGTGGCAACTAGAGAGATTTATCCATTGAAATCGGAGACTAGAATTTCTGCGAATTTTAAAATTAAAAAATAA

Protein sequence

MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKSETRISANFKIKK
Homology
[Close]
BLAST of CsGy2G008620 vs. NCBI nr
Match: KGN50267.1 (hypothetical protein Csa_5G162650 [Cucumis sativus])

HSP 1 Score: 102.8 bits (255), Expect = 4.9e-19
Identity = 52/70 (74.29%), Postives = 56/70 (80.00%), Query Frame = 0 

Query: 1  MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
          MKTKY III+ DNF CGSSREH  V LG S  VA+V ESYAHIF TANSVATREIYPL+S
Sbjct: 1  MKTKYSIIIVGDNFGCGSSREHVPVVLGVSGAVAVVAESYAHIFLTANSVATREIYPLES 60

Query: 61 ETRISANFKI 71
          ETRIS   K+
Sbjct: 61 ETRISRILKL 70

[Close]
BLAST of CsGy2G008620 vs. NCBI nr
Match: XP_004152394.1 (PREDICTED: 3-isopropylmalate dehydratase small subunit 3 [Cucumis sativus] >KGN50272.1 hypothetical protein Csa_5G165170 [Cucumis sativus])

HSP 1 Score: 91.7 bits (226), Expect = 1.1e-15
Identity = 51/69 (73.91%), Postives = 53/69 (76.81%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MKTKY III  DNF CGSSREHA VALGAS  VA+V ESYA IFF  NSVAT EIYPL+S
Sbjct: 129 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 188

Query: 61  ETRISANFK 70
           ETRI    K
Sbjct: 189 ETRICEECK 196

[Close]
BLAST of CsGy2G008620 vs. NCBI nr
Match: XP_008437054.1 (PREDICTED: 3-isopropylmalate dehydratase small subunit 3-like [Cucumis melo])

HSP 1 Score: 91.7 bits (226), Expect = 1.1e-15
Identity = 50/64 (78.12%), Postives = 52/64 (81.25%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MKTKY III  DNF CGSSREHA VALGAS  VA+V ESYA IFF  NSVAT EIYPL+S
Sbjct: 134 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 193

Query: 61  ETRI 65
           ETRI
Sbjct: 194 ETRI 196

[Close]
BLAST of CsGy2G008620 vs. NCBI nr
Match: XP_022922281.1 (3-isopropylmalate dehydratase small subunit 3-like [Cucurbita moschata])

HSP 1 Score: 89.4 bits (220), Expect = 5.6e-15
Identity = 49/64 (76.56%), Postives = 51/64 (79.69%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MKTKY III  DNF CGSSREHA VALGAS  VA+V ESYA IFF  NSVAT EIYPL+S
Sbjct: 134 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 193

Query: 61  ETRI 65
           E RI
Sbjct: 194 EIRI 196

[Close]
BLAST of CsGy2G008620 vs. NCBI nr
Match: XP_022972989.1 (3-isopropylmalate dehydratase small subunit 3-like [Cucurbita maxima])

HSP 1 Score: 89.4 bits (220), Expect = 5.6e-15
Identity = 49/64 (76.56%), Postives = 51/64 (79.69%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MKTKY III  DNF CGSSREHA VALGAS  VA+V ESYA IFF  NSVAT EIYPL+S
Sbjct: 134 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 193

Query: 61  ETRI 65
           E RI
Sbjct: 194 EIRI 196

[Close]
BLAST of CsGy2G008620 vs. TAIR10
Match: AT2G43090.1 (Aconitase/3-isopropylmalate dehydratase protein)

HSP 1 Score: 79.3 bits (194), Expect = 1.0e-15
Identity = 42/64 (65.62%), Postives = 48/64 (75.00%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MKTKY III  +NF CGSSREHA V LGA+   A+V +SYA IFF  NSVAT E+YPL S
Sbjct: 127 MKTKYSIIIGGENFGCGSSREHAPVCLGAAGAKAVVAQSYARIFF-RNSVATGEVYPLDS 186

Query: 61  ETRI 65
           E R+
Sbjct: 187 EVRV 189

[Close]
BLAST of CsGy2G008620 vs. TAIR10
Match: AT3G58990.1 (isopropylmalate isomerase 1)

HSP 1 Score: 78.2 bits (191), Expect = 2.3e-15
Identity = 42/64 (65.62%), Postives = 48/64 (75.00%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MK+KY +II  DNF CGSSREHA V LGA+   A+V ESYA IFF  N VAT EI+PL+S
Sbjct: 130 MKSKYSVIIGGDNFGCGSSREHAPVCLGAAGAKAVVAESYARIFF-RNCVATGEIFPLES 189

Query: 61  ETRI 65
           E RI
Sbjct: 190 EVRI 192

[Close]
BLAST of CsGy2G008620 vs. TAIR10
Match: AT2G43100.1 (isopropylmalate isomerase 2)

HSP 1 Score: 75.5 bits (184), Expect = 1.5e-14
Identity = 41/68 (60.29%), Postives = 49/68 (72.06%), Query Frame = 0 

Query: 2   KTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKSE 61
           ++KY III  +NF CGSSREHA V LGA+   AIV ESYA IFF  NSVAT E++PL+SE
Sbjct: 134 RSKYSIIIGGENFGCGSSREHAPVCLGAAGAKAIVAESYARIFF-RNSVATGEVFPLESE 193

Query: 62  TRISANFK 70
            R+    K
Sbjct: 194 VRVCEECK 200

[Close]
BLAST of CsGy2G008620 vs. Swiss-Prot
Match: sp|Q9ZW85|LEUD3_ARATH (3-isopropylmalate dehydratase small subunit 3 OS=Arabidopsis thaliana OX=3702 GN=At2g43090 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.9e-14
Identity = 42/64 (65.62%), Postives = 48/64 (75.00%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MKTKY III  +NF CGSSREHA V LGA+   A+V +SYA IFF  NSVAT E+YPL S
Sbjct: 127 MKTKYSIIIGGENFGCGSSREHAPVCLGAAGAKAVVAQSYARIFF-RNSVATGEVYPLDS 186

Query: 61  ETRI 65
           E R+
Sbjct: 187 EVRV 189

[Close]
BLAST of CsGy2G008620 vs. Swiss-Prot
Match: sp|Q9LYT7|LEUD2_ARATH (3-isopropylmalate dehydratase small subunit 2 OS=Arabidopsis thaliana OX=3702 GN=IPMI1 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.2e-14
Identity = 42/64 (65.62%), Postives = 48/64 (75.00%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MK+KY +II  DNF CGSSREHA V LGA+   A+V ESYA IFF  N VAT EI+PL+S
Sbjct: 130 MKSKYSVIIGGDNFGCGSSREHAPVCLGAAGAKAVVAESYARIFF-RNCVATGEIFPLES 189

Query: 61  ETRI 65
           E RI
Sbjct: 190 EVRI 192

[Close]
BLAST of CsGy2G008620 vs. Swiss-Prot
Match: sp|Q9ZW84|LEUD1_ARATH (3-isopropylmalate dehydratase small subunit 1 OS=Arabidopsis thaliana OX=3702 GN=IPMI2 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.7e-13
Identity = 41/68 (60.29%), Postives = 49/68 (72.06%), Query Frame = 0 

Query: 2   KTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKSE 61
           ++KY III  +NF CGSSREHA V LGA+   AIV ESYA IFF  NSVAT E++PL+SE
Sbjct: 134 RSKYSIIIGGENFGCGSSREHAPVCLGAAGAKAIVAESYARIFF-RNSVATGEVFPLESE 193

Query: 62  TRISANFK 70
            R+    K
Sbjct: 194 VRVCEECK 200

[Close]
BLAST of CsGy2G008620 vs. Swiss-Prot
Match: sp|Q0QLE1|DMDB_EUBBA (2,3-dimethylmalate dehydratase small subunit OS=Eubacterium barkeri OX=1528 GN=DmdB PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 3.6e-05
Identity = 21/39 (53.85%), Postives = 29/39 (74.36%), Query Frame = 0 

Query: 7  IIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFF 46
          II+ DDNF CGSSREHA +++ AS V  ++  S+A IF+
Sbjct: 54 IIVADDNFGCGSSREHAPISIKASGVSCVIANSFARIFY 92

[Close]
BLAST of CsGy2G008620 vs. Swiss-Prot
Match: sp|B1H0A5|LEUD_UNCTG (3-isopropylmalate dehydratase small subunit OS=Uncultured termite group 1 bacterium phylotype Rs-D17 OX=471821 GN=leuD PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 3.6e-05
Identity = 21/39 (53.85%), Postives = 30/39 (76.92%), Query Frame = 0 

Query: 7  IIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFF 46
          II+ ++NF CGSSREHA +A+ AS + A++  S+A IFF
Sbjct: 57 IIVAENNFGCGSSREHAPIAIKASGISAVIANSFARIFF 95

[Close]
BLAST of CsGy2G008620 vs. TrEMBL
Match: tr|A0A0A0KPA8|A0A0A0KPA8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G162650 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 3.2e-19
Identity = 52/70 (74.29%), Postives = 56/70 (80.00%), Query Frame = 0 

Query: 1  MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
          MKTKY III+ DNF CGSSREH  V LG S  VA+V ESYAHIF TANSVATREIYPL+S
Sbjct: 1  MKTKYSIIIVGDNFGCGSSREHVPVVLGVSGAVAVVAESYAHIFLTANSVATREIYPLES 60

Query: 61 ETRISANFKI 71
          ETRIS   K+
Sbjct: 61 ETRISRILKL 70

[Close]
BLAST of CsGy2G008620 vs. TrEMBL
Match: tr|A0A1S3AT32|A0A1S3AT32_CUCME (3-isopropylmalate dehydratase small subunit 3-like OS=Cucumis melo OX=3656 GN=LOC103482595 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.4e-16
Identity = 50/64 (78.12%), Postives = 52/64 (81.25%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MKTKY III  DNF CGSSREHA VALGAS  VA+V ESYA IFF  NSVAT EIYPL+S
Sbjct: 134 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 193

Query: 61  ETRI 65
           ETRI
Sbjct: 194 ETRI 196

[Close]
BLAST of CsGy2G008620 vs. TrEMBL
Match: tr|A0A0A0KPB2|A0A0A0KPB2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G165170 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.4e-16
Identity = 51/69 (73.91%), Postives = 53/69 (76.81%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MKTKY III  DNF CGSSREHA VALGAS  VA+V ESYA IFF  NSVAT EIYPL+S
Sbjct: 129 MKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFF-RNSVATGEIYPLES 188

Query: 61  ETRISANFK 70
           ETRI    K
Sbjct: 189 ETRICEECK 196

[Close]
BLAST of CsGy2G008620 vs. TrEMBL
Match: tr|A0A2C9WIS8|A0A2C9WIS8_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G082700 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-14
Identity = 47/69 (68.12%), Postives = 52/69 (75.36%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MKTKY I+I  DNF CGSSREHA VALGA+ V A++ ESYA IFF  NSVAT EIYPL+S
Sbjct: 130 MKTKYTIVIAGDNFGCGSSREHAPVALGAAGVAAVIAESYARIFF-RNSVATGEIYPLES 189

Query: 61  ETRISANFK 70
           E RI    K
Sbjct: 190 EVRICEECK 197

[Close]
BLAST of CsGy2G008620 vs. TrEMBL
Match: tr|A0A218XNA1|A0A218XNA1_PUNGR (Uncharacterized protein OS=Punica granatum OX=22663 GN=CDL15_Pgr021510 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.8e-14
Identity = 48/69 (69.57%), Postives = 52/69 (75.36%), Query Frame = 0 

Query: 1   MKTKYFIIIIDDNFSCGSSREHALVALGASDVVAIVDESYAHIFFTANSVATREIYPLKS 60
           MKTKY I+I  DNF CGSSREHA VALGA+  VA+V ESYA IFF  NSVAT EIYPL+S
Sbjct: 133 MKTKYSIVIGGDNFGCGSSREHAPVALGAAGAVAVVAESYARIFF-RNSVATGEIYPLES 192

Query: 61  ETRISANFK 70
           E RI    K
Sbjct: 193 EVRICEECK 200

The following BLAST results are available for this feature:
BLAST of CsGy2G008620 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN50267.14.9e-1974.29hypothetical protein Csa_5G162650 [Cucumis sativus][more]
XP_004152394.11.1e-1573.91PREDICTED: 3-isopropylmalate dehydratase small subunit 3 [Cucumis sativus] >KGN5... [more]
XP_008437054.11.1e-1578.13PREDICTED: 3-isopropylmalate dehydratase small subunit 3-like [Cucumis melo][more]
XP_022922281.15.6e-1576.563-isopropylmalate dehydratase small subunit 3-like [Cucurbita moschata][more]
XP_022972989.15.6e-1576.563-isopropylmalate dehydratase small subunit 3-like [Cucurbita maxima][more]
BLAST of CsGy2G008620 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G43090.11.0e-1565.63Aconitase/3-isopropylmalate dehydratase protein[more]
AT3G58990.12.3e-1565.63isopropylmalate isomerase 1[more]
AT2G43100.11.5e-1460.29isopropylmalate isomerase 2[more]
BLAST of CsGy2G008620 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9ZW85|LEUD3_ARATH1.9e-1465.633-isopropylmalate dehydratase small subunit 3 OS=Arabidopsis thaliana OX=3702 GN... [more]
sp|Q9LYT7|LEUD2_ARATH4.2e-1465.633-isopropylmalate dehydratase small subunit 2 OS=Arabidopsis thaliana OX=3702 GN... [more]
sp|Q9ZW84|LEUD1_ARATH2.7e-1360.293-isopropylmalate dehydratase small subunit 1 OS=Arabidopsis thaliana OX=3702 GN... [more]
sp|Q0QLE1|DMDB_EUBBA3.6e-0553.852,3-dimethylmalate dehydratase small subunit OS=Eubacterium barkeri OX=1528 GN=D... [more]
sp|B1H0A5|LEUD_UNCTG3.6e-0553.853-isopropylmalate dehydratase small subunit OS=Uncultured termite group 1 bacter... [more]
BLAST of CsGy2G008620 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0KPA8|A0A0A0KPA8_CUCSA3.2e-1974.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G162650 PE=4 SV=1[more]
tr|A0A1S3AT32|A0A1S3AT32_CUCME7.4e-1678.133-isopropylmalate dehydratase small subunit 3-like OS=Cucumis melo OX=3656 GN=LO... [more]
tr|A0A0A0KPB2|A0A0A0KPB2_CUCSA7.4e-1673.91Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G165170 PE=4 SV=1[more]
tr|A0A2C9WIS8|A0A2C9WIS8_MANES1.1e-1468.12Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G082700 PE=4 SV=... [more]
tr|A0A218XNA1|A0A218XNA1_PUNGR1.8e-1469.57Uncharacterized protein OS=Punica granatum OX=22663 GN=CDL15_Pgr021510 PE=4 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR015928Aconitase/3IPM_dehydase_swvl
IPR000573AconitaseA/IPMdHydase_ssu_swvl
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016836 hydro-lyase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G008620.1CsGy2G008620.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000573Aconitase A/isopropylmalate dehydratase small subunit, swivel domainPFAMPF00694Aconitase_Ccoord: 6..50
e-value: 7.9E-10
score: 39.1
IPR015928Aconitase/3-isopropylmalate dehydratase, swivelGENE3DG3DSA:3.20.19.10coord: 2..70
e-value: 1.5E-12
score: 49.4
NoneNo IPR availablePANTHERPTHR43345:SF33-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 1-RELATEDcoord: 1..67
NoneNo IPR availablePANTHERPTHR43345FAMILY NOT NAMEDcoord: 1..67
NoneNo IPR availableSUPERFAMILYSSF52016LeuD/IlvD-likecoord: 4..46

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsGy2G008620Cucurbita maxima (Rimu)cgybcmaB205
CsGy2G008620Cucurbita maxima (Rimu)cgybcmaB259
CsGy2G008620Cucurbita moschata (Rifu)cgybcmoB199
CsGy2G008620Cucurbita moschata (Rifu)cgybcmoB240
CsGy2G008620Cucurbita pepo (Zucchini)cgybcpeB171
CsGy2G008620Cucurbita pepo (Zucchini)cgybcpeB222
CsGy2G008620Cucumber (Chinese Long) v2cgybcuB056
CsGy2G008620Bottle gourd (USVL1VR-Ls)cgyblsiB089
CsGy2G008620Melon (DHL92) v3.5.1cgybmeB124
CsGy2G008620Melon (DHL92) v3.6.1cgybmedB122
CsGy2G008620Watermelon (Charleston Gray)cgybwcgB097
CsGy2G008620Watermelon (97103) v1cgybwmB150
CsGy2G008620Wild cucumber (PI 183967)cgybcpiB056
CsGy2G008620Silver-seed gourdcarcgybB0696
CsGy2G008620Cucumber (Chinese Long) v3cgybcucB064
CsGy2G008620Watermelon (97103) v2cgybwmbB100
CsGy2G008620Wax gourdcgybwgoB134