CsGy2G008320 (gene) Cucumber (Gy14) v2

Overview
NameCsGy2G008320
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionPlant invertase/pectin methylesterase inhibitor
LocationChr2 : 7291276 .. 7291583 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCTCGAGCAGGGGTGTCAGTGATGATAATAGAGGCAAAAAAAGTGGTAGGACTCCTGGGGGATTGACGAACAACAAGCGGATGAAAAGGAGAAACAAGGCAGTCCAAGCTGTGTGAAGGTGTTTGAAGCCACAATTGATGAGCTTCACAGGTCGTATGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAAATGGGGGATCTAACGACGTGGGTCAACACTGCACTGACGAATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGTTGCCTCTC

mRNA sequence

ATGGGCTCGAGCAGGGGTGTCAGTGATGATAATAGAGGCAAAAAAAGTGGTAGGACTCCTGGGGGATTGACGAACAACAAGCGGATGAAAAGGAGAAACAAGGCAGTCCAAGCTGTGTTTGAAGCCACAATTGATGAGCTTCACAGGTCGTATGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAAATGGGGGATCTAACGACGTGGGTCAACACTGCACTGACGAATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGTTGCCTCTC

Coding sequence (CDS)

ATGGGCTCGAGCAGGGGTGTCAGTGATGATAATAGAGGCAAAAAAAGTGGTAGGACTCCTGGGGGATTGACGAACAACAAGCGGATGAAAAGGAGAAACAAGGCAGTCCAAGCTGTGTTTGAAGCCACAATTGATGAGCTTCACAGGTCGTATGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAAATGGGGGATCTAACGACGTGGGTCAACACTGCACTGACGAATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGTTGCCTCTC

Protein sequence

MGSSRGVSDDNRGKKSGRTPGGLTNNKRMKRRNKAVQAVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNLLPL
Homology
[Close]
BLAST of CsGy2G008320 vs. NCBI nr
Match: XP_008451567.1 (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 123.2 bits (308), Expect = 4.8e-25
Identity = 64/89 (71.91%), Postives = 71/89 (79.78%), Query Frame = 0 

Query: 14  KKSGRTPGGLTNNKRMKRRNKAV----QAVFEATIDELHRSYGVLRRLSRRNFDAQMGDL 73
           KK     G L NNKRMK RN+A       VF+A IDELHRS GVLRRLSRRNFD+QMGDL
Sbjct: 77  KKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGVLRRLSRRNFDSQMGDL 136

Query: 74  TTWVNTALTNEDTCIEGFEGERGKVVNLL 99
           TTWV+ ALT+EDTC+EGFEGERGKVV+LL
Sbjct: 137 TTWVSAALTDEDTCVEGFEGERGKVVSLL 165

[Close]
BLAST of CsGy2G008320 vs. NCBI nr
Match: XP_011659361.1 (PREDICTED: 21 kDa protein [Cucumis sativus] >KGN44941.1 hypothetical protein Csa_7G397050 [Cucumis sativus])

HSP 1 Score: 122.9 bits (307), Expect = 6.3e-25
Identity = 65/89 (73.03%), Postives = 69/89 (77.53%), Query Frame = 0 

Query: 14  KKSGRTPGGLTNNKRMKRRNKAV----QAVFEATIDELHRSYGVLRRLSRRNFDAQMGDL 73
           KK     G L NNKRMK RN+A       VFEA IDELHRS GVLRRLSRRNFDAQMGDL
Sbjct: 77  KKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRRNFDAQMGDL 136

Query: 74  TTWVNTALTNEDTCIEGFEGERGKVVNLL 99
           TTWV+ ALT+EDTC+EGFEGE GKVV LL
Sbjct: 137 TTWVSAALTDEDTCVEGFEGEEGKVVTLL 165

[Close]
BLAST of CsGy2G008320 vs. NCBI nr
Match: XP_022960074.1 (pectinesterase inhibitor 6-like [Cucurbita moschata])

HSP 1 Score: 115.2 bits (287), Expect = 1.3e-22
Identity = 56/80 (70.00%), Postives = 66/80 (82.50%), Query Frame = 0 

Query: 23  LTNNKRMKRRNKAVQA----VFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALT 82
           L NN+RMK RN+A  +    VFE  IDELHRS GVLRRLSR  FDAQMGDLTTWV+ ALT
Sbjct: 148 LKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALT 207

Query: 83  NEDTCIEGFEGERGKVVNLL 99
           +EDTC++GFEGERG++VNL+
Sbjct: 208 DEDTCVDGFEGERGRLVNLI 227

[Close]
BLAST of CsGy2G008320 vs. NCBI nr
Match: XP_023004663.1 (pectinesterase inhibitor 6 [Cucurbita maxima])

HSP 1 Score: 115.2 bits (287), Expect = 1.3e-22
Identity = 56/80 (70.00%), Postives = 66/80 (82.50%), Query Frame = 0 

Query: 23  LTNNKRMKRRNKAVQA----VFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALT 82
           L NN+RMK RN+A  +    VFE  IDELHRS GVLRRLSR  FDAQMGDLTTWV+ ALT
Sbjct: 86  LKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALT 145

Query: 83  NEDTCIEGFEGERGKVVNLL 99
           +EDTC++GFEGERG++VNL+
Sbjct: 146 DEDTCVDGFEGERGRLVNLI 165

[Close]
BLAST of CsGy2G008320 vs. NCBI nr
Match: XP_023514751.1 (pectinesterase inhibitor 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 115.2 bits (287), Expect = 1.3e-22
Identity = 56/80 (70.00%), Postives = 66/80 (82.50%), Query Frame = 0 

Query: 23  LTNNKRMKRRNKAVQA----VFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALT 82
           L NN+RMK RN+A  +    VFE  IDELHRS GVLRRLSR  FDAQMGDLTTWV+ ALT
Sbjct: 86  LKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALT 145

Query: 83  NEDTCIEGFEGERGKVVNLL 99
           +EDTC++GFEGERG++VNL+
Sbjct: 146 DEDTCVDGFEGERGRLVNLI 165

[Close]
BLAST of CsGy2G008320 vs. TAIR10
Match: AT3G62820.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 57.4 bits (137), Expect = 5.9e-09
Identity = 26/65 (40.00%), Postives = 41/65 (63.08%), Query Frame = 0 

Query: 24  TNNKRMKRRNKAVQAVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTC 83
           T  KR +      + + + ++D LH+S  VLR L    F  QM DL TW++++LT++DTC
Sbjct: 91  TIGKRDRIALSDCRELLQDSLDSLHKSLAVLRTLRASEFQQQMSDLATWLSSSLTDKDTC 150

Query: 84  IEGFE 89
           ++GFE
Sbjct: 151 LDGFE 155

[Close]
BLAST of CsGy2G008320 vs. TAIR10
Match: AT2G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 57.0 bits (136), Expect = 7.7e-09
Identity = 23/50 (46.00%), Postives = 36/50 (72.00%), Query Frame = 0 

Query: 39  VFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFE 89
           +F  ++D L++S  VLR L+   F  Q+ DL TW++ ALT++DTC++GFE
Sbjct: 121 LFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDDTCLDGFE 170

[Close]
BLAST of CsGy2G008320 vs. TAIR10
Match: AT5G20740.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 51.2 bits (121), Expect = 4.2e-07
Identity = 23/52 (44.23%), Postives = 35/52 (67.31%), Query Frame = 0 

Query: 43  TIDELHRSYGVLRRL-----SRRNFDAQMGDLTTWVNTALTNEDTCIEGFEG 90
           ++DEL R+ GVL+ L     S + F  QM +  TW + ALT++DTC++GF+G
Sbjct: 111 SVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQG 162

[Close]
BLAST of CsGy2G008320 vs. TAIR10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 45.1 bits (105), Expect = 3.0e-05
Identity = 20/55 (36.36%), Postives = 33/55 (60.00%), Query Frame = 0 

Query: 44  IDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNLL 99
           +++LH   G+       +  A  G+L TW++ A++N+DTC+EGFEG   K   L+
Sbjct: 126 MNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAAMSNQDTCLEGFEGTERKYEELI 180

[Close]
BLAST of CsGy2G008320 vs. TAIR10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 43.9 bits (102), Expect = 6.8e-05
Identity = 15/31 (48.39%), Postives = 24/31 (77.42%), Query Frame = 0 

Query: 68  DLTTWVNTALTNEDTCIEGFEGERGKVVNLL 99
           DL TW++ AL+N+DTC++GFEG  G +  ++
Sbjct: 118 DLRTWISAALSNQDTCLDGFEGTNGIIKKIV 148

[Close]
BLAST of CsGy2G008320 vs. Swiss-Prot
Match: sp|O22244|PMEI6_ARATH (Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.4e-07
Identity = 23/50 (46.00%), Postives = 36/50 (72.00%), Query Frame = 0 

Query: 39  VFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFE 89
           +F  ++D L++S  VLR L+   F  Q+ DL TW++ ALT++DTC++GFE
Sbjct: 121 LFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDDTCLDGFE 170

[Close]
BLAST of CsGy2G008320 vs. Swiss-Prot
Match: sp|Q84WE4|PMEI3_ARATH (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 7.6e-06
Identity = 23/52 (44.23%), Postives = 35/52 (67.31%), Query Frame = 0 

Query: 43  TIDELHRSYGVLRRL-----SRRNFDAQMGDLTTWVNTALTNEDTCIEGFEG 90
           ++DEL R+ GVL+ L     S + F  QM +  TW + ALT++DTC++GF+G
Sbjct: 111 SVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQG 162

[Close]
BLAST of CsGy2G008320 vs. Swiss-Prot
Match: sp|Q9M9W7|PME22_ARATH (Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3702 GN=PME22 PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.5e-04
Identity = 20/55 (36.36%), Postives = 33/55 (60.00%), Query Frame = 0 

Query: 44  IDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNLL 99
           +++LH   G+       +  A  G+L TW++ A++N+DTC+EGFEG   K   L+
Sbjct: 126 MNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAAMSNQDTCLEGFEGTERKYEELI 180

[Close]
BLAST of CsGy2G008320 vs. TrEMBL
Match: tr|A0A1S3BSW5|A0A1S3BSW5_CUCME (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.2e-25
Identity = 64/89 (71.91%), Postives = 71/89 (79.78%), Query Frame = 0 

Query: 14  KKSGRTPGGLTNNKRMKRRNKAV----QAVFEATIDELHRSYGVLRRLSRRNFDAQMGDL 73
           KK     G L NNKRMK RN+A       VF+A IDELHRS GVLRRLSRRNFD+QMGDL
Sbjct: 77  KKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGVLRRLSRRNFDSQMGDL 136

Query: 74  TTWVNTALTNEDTCIEGFEGERGKVVNLL 99
           TTWV+ ALT+EDTC+EGFEGERGKVV+LL
Sbjct: 137 TTWVSAALTDEDTCVEGFEGERGKVVSLL 165

[Close]
BLAST of CsGy2G008320 vs. TrEMBL
Match: tr|A0A0A0KAX0|A0A0A0KAX0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 4.2e-25
Identity = 65/89 (73.03%), Postives = 69/89 (77.53%), Query Frame = 0 

Query: 14  KKSGRTPGGLTNNKRMKRRNKAV----QAVFEATIDELHRSYGVLRRLSRRNFDAQMGDL 73
           KK     G L NNKRMK RN+A       VFEA IDELHRS GVLRRLSRRNFDAQMGDL
Sbjct: 77  KKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSRRNFDAQMGDL 136

Query: 74  TTWVNTALTNEDTCIEGFEGERGKVVNLL 99
           TTWV+ ALT+EDTC+EGFEGE GKVV LL
Sbjct: 137 TTWVSAALTDEDTCVEGFEGEEGKVVTLL 165

[Close]
BLAST of CsGy2G008320 vs. TrEMBL
Match: tr|A0A0A0LDI5|A0A0A0LDI5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G560260 PE=4 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 6.9e-20
Identity = 62/101 (61.39%), Postives = 67/101 (66.34%), Query Frame = 0 

Query: 1  MGSSRGVSDDNRGKKSGRTPGGLTNNKRMKRRNKAVQAV---FEATIDELHRSYGVLRRL 60
          MGSSRGV    + KK     G LTNNKRMK RNKA+QAV   F A IDE HR        
Sbjct: 1  MGSSRGVGAIAKAKKVAGLLGRLTNNKRMKGRNKAIQAVWRCFGAVIDEFHR-------- 60

Query: 61 SRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNLL 99
           +RNFDAQMGDLTTWV   L +EDTCI  F+GERGKVVN L
Sbjct: 61 -QRNFDAQMGDLTTWVRAMLMDEDTCIRRFKGERGKVVNFL 92

[Close]
BLAST of CsGy2G008320 vs. TrEMBL
Match: tr|A0A0A0L1N4|A0A0A0L1N4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G615740 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.0e-18
Identity = 47/60 (78.33%), Postives = 52/60 (86.67%), Query Frame = 0 

Query: 39 VFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNLL 98
          VFEA IDELH S+GVLRRLSRRNFD QMG LTTWV+ ALTNED C++GFEG R K+VNLL
Sbjct: 28 VFEAAIDELHMSFGVLRRLSRRNFDTQMGYLTTWVSIALTNEDICVKGFEGGREKIVNLL 87

[Close]
BLAST of CsGy2G008320 vs. TrEMBL
Match: tr|G7IF58|G7IF58_MEDTR (Plant invertase/pectin methylesterase inhibitor OS=Medicago truncatula OX=3880 GN=11420020 PE=4 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 6.7e-15
Identity = 43/81 (53.09%), Postives = 59/81 (72.84%), Query Frame = 0 

Query: 23  LTNNKRMKRRNK-----AVQAVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTAL 82
           LT + R++ RNK      V+ + +A +DELHRS  VLRRLSRR F  QMGDL TW++ AL
Sbjct: 40  LTRHGRLRGRNKVALLDCVETIADA-LDELHRSLNVLRRLSRRTFGTQMGDLNTWISAAL 99

Query: 83  TNEDTCIEGFEGERGKVVNLL 99
           T++DTC++GF+GE G+ + LL
Sbjct: 100 TDQDTCLDGFQGENGRKIQLL 119

The following BLAST results are available for this feature:
BLAST of CsGy2G008320 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008451567.14.8e-2571.91PREDICTED: 21 kDa protein [Cucumis melo][more]
XP_011659361.16.3e-2573.03PREDICTED: 21 kDa protein [Cucumis sativus] >KGN44941.1 hypothetical protein Csa... [more]
XP_022960074.11.3e-2270.00pectinesterase inhibitor 6-like [Cucurbita moschata][more]
XP_023004663.11.3e-2270.00pectinesterase inhibitor 6 [Cucurbita maxima][more]
XP_023514751.11.3e-2270.00pectinesterase inhibitor 6 [Cucurbita pepo subsp. pepo][more]
BLAST of CsGy2G008320 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G62820.15.9e-0940.00Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT2G47670.17.7e-0946.00Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G20740.14.2e-0744.23Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT3G05620.13.0e-0536.36Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G43270.16.8e-0548.39Plant invertase/pectin methylesterase inhibitor superfamily[more]
BLAST of CsGy2G008320 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|O22244|PMEI6_ARATH1.4e-0746.00Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1[more]
sp|Q84WE4|PMEI3_ARATH7.6e-0644.23Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1[more]
sp|Q9M9W7|PME22_ARATH5.5e-0436.36Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3... [more]
BLAST of CsGy2G008320 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3BSW5|A0A1S3BSW5_CUCME3.2e-2571.9121 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1[more]
tr|A0A0A0KAX0|A0A0A0KAX0_CUCSA4.2e-2573.03Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 SV=1[more]
tr|A0A0A0LDI5|A0A0A0LDI5_CUCSA6.9e-2061.39Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G560260 PE=4 SV=1[more]
tr|A0A0A0L1N4|A0A0A0L1N4_CUCSA1.0e-1878.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G615740 PE=4 SV=1[more]
tr|G7IF58|G7IF58_MEDTR6.7e-1553.09Plant invertase/pectin methylesterase inhibitor OS=Medicago truncatula OX=3880 G... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR035513Invertase/methylesterase_inhib
IPR006501Pectinesterase_inhib_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G008320.1CsGy2G008320.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 38..93
e-value: 4.8E-9
score: 36.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR31080:SF16PECTIN METHYLESTERASE INHIBITOR 6-RELATEDcoord: 38..98
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 38..98
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 23..98
e-value: 1.33953E-14
score: 63.9965
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILYSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 24..97