CsGy2G003800 (gene) Cucumber (Gy14) v2

Overview
NameCsGy2G003800
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionKunitz-type trypsin inhibitor
LocationChr2 : 2563151 .. 2563738 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGATGCTACATAAATCACTAGCCATCTTTGGCTATGCATGTCTCTTCATGGCCATAACTTCCACAGCTCAATCGCCTCCAGTGCTCGACACCAACGGTCAACCTCTGCAACGCGGTGTCGAGTACTACATCTCACCTGCAATTACTGACGTTGGGGGCAATCTCACCCTAAAAAGTCGTAGTAACGCTCCGTGCCCGCTCTTCGTTGGCCAGGAACCCGTTACTTCGACAAATATCGGTCTCCCAGTCACCTTTAGACCCACTGAGGAGGGCATAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACATGTGTAACATCAACACAATGGAGAGTGGATGCAACTGAGTCTGATACCGGAAGAAGGTTCGTGGGGATCGGGGATGAGGACGGACCTGCTGGGATCTTTGGGATCAGTAGGGACAACGGAGCTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGATGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGGTGGCTTTGGATGGTGATGCTTTTCCTTTTGAGTTTATCAAAGCTTGA

mRNA sequence

ATGACGATGCTACATAAATCACTAGCCATCTTTGGCTATGCATGTCTCTTCATGGCCATAACTTCCACAGCTCAATCGCCTCCAGTGCTCGACACCAACGGTCAACCTCTGCAACGCGGTGTCGAGTACTACATCTCACCTGCAATTACTGACGTTGGGGGCAATCTCACCCTAAAAAGTCGTAGTAACGCTCCGTGCCCGCTCTTCGTTGGCCAGGAACCCGTTACTTCGACAAATATCGGTCTCCCAGTCACCTTTAGACCCACTGAGGAGGGCATAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACATGTGTAACATCAACACAATGGAGAGTGGATGCAACTGAGTCTGATACCGGAAGAAGGTTCGTGGGGATCGGGGATGAGGACGGACCTGCTGGGATCTTTGGGATCAGTAGGGACAACGGAGCTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGATGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGGTGGCTTTGGATGGTGATGCTTTTCCTTTTGAGTTTATCAAAGCTTGA

Coding sequence (CDS)

ATGACGATGCTACATAAATCACTAGCCATCTTTGGCTATGCATGTCTCTTCATGGCCATAACTTCCACAGCTCAATCGCCTCCAGTGCTCGACACCAACGGTCAACCTCTGCAACGCGGTGTCGAGTACTACATCTCACCTGCAATTACTGACGTTGGGGGCAATCTCACCCTAAAAAGTCGTAGTAACGCTCCGTGCCCGCTCTTCGTTGGCCAGGAACCCGTTACTTCGACAAATATCGGTCTCCCAGTCACCTTTAGACCCACTGAGGAGGGCATAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACATGTGTAACATCAACACAATGGAGAGTGGATGCAACTGAGTCTGATACCGGAAGAAGGTTCGTGGGGATCGGGGATGAGGACGGACCTGCTGGGATCTTTGGGATCAGTAGGGACAACGGAGCTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGATGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGGTGGCTTTGGATGGTGATGCTTTTCCTTTTGAGTTTATCAAAGCTTGA

Protein sequence

MTMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA
Homology
[Close]
BLAST of CsGy2G003800 vs. NCBI nr
Match: XP_004139192.1 (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 396.7 bits (1018), Expect = 4.4e-107
Identity = 193/195 (98.97%), Postives = 193/195 (98.97%), Query Frame = 0 

Query: 1   MTMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60
           M MLHKSLAIFGYACLFMAITSTAQ PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120

Query: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 VALDGDAFPFEFIKA 196
           VALDGDAFPFEFIKA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]
BLAST of CsGy2G003800 vs. NCBI nr
Match: KGN60879.1 (hypothetical protein Csa_2G021500 [Cucumis sativus])

HSP 1 Score: 365.5 bits (937), Expect = 1.1e-97
Identity = 177/178 (99.44%), Postives = 177/178 (99.44%), Query Frame = 0 

Query: 18  MAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 77
           MAITSTAQ PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 78  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 137
           TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 138 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 196
           DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]
BLAST of CsGy2G003800 vs. NCBI nr
Match: XP_022946294.1 (miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita moschata])

HSP 1 Score: 313.9 bits (803), Expect = 3.8e-82
Identity = 147/193 (76.17%), Postives = 166/193 (86.01%), Query Frame = 0 

Query: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 62
           MLH SLAIF +  +FMAITSTAQ PPVLDT+GQPL+RGVEYYI PAITDV GNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 122
           NAPCPL+VGQEP+ STNIGLPVTF P   G DII+E    N+VFQA STC+TSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182
            ES TGRRFVG+G+++GP GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+A
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFIKA 196
           LDG+AFPFEF+KA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]
BLAST of CsGy2G003800 vs. NCBI nr
Match: XP_023545477.1 (miraculin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 313.5 bits (802), Expect = 4.9e-82
Identity = 148/193 (76.68%), Postives = 164/193 (84.97%), Query Frame = 0 

Query: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 62
           MLH SLAIF Y  +FMAITSTAQ PPVLDT+GQPLQRGVEYYI PAITDV GNLTLKSRS
Sbjct: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 122
           NAPCPL+VGQEPV STNIGLPVTF P   G DII+E    N+ FQA STC+TSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182
            ES TGRRFVG+G+++ P GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+A
Sbjct: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFIKA 196
           LDG+AFPFEF+KA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]
BLAST of CsGy2G003800 vs. NCBI nr
Match: XP_022999136.1 (miraculin-like [Cucurbita maxima])

HSP 1 Score: 310.1 bits (793), Expect = 5.4e-81
Identity = 145/193 (75.13%), Postives = 165/193 (85.49%), Query Frame = 0 

Query: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 62
           MLH SLAIF Y  +F+AITSTAQ PPVLDT+GQPL+RGVEYYI PAIT+V GNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 122
           NAPCPL+VGQEPV STNIGLPVTF P   G DII+E    N+ FQA STC+TSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182
            ES TGRRFVG+G+++GP GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+A
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFIKA 196
           LDG+AFPFEF+KA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]
BLAST of CsGy2G003800 vs. TAIR10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 78.2 bits (191), Expect = 6.3e-15
Identity = 60/191 (31.41%), Postives = 94/191 (49.21%), Query Frame = 0 

Query: 14  ACLFMAITSTAQSPP--VLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVG 73
           + +F A ++   +P   VLD  G P+Q  V+YYI PA    GG L   +R+ +   L + 
Sbjct: 15  SAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLN 74

Query: 74  QEPVTSTN---IGLPVTFRPTEEGIDIIDEGTSLNIVFQA-LSTCVTSTQWRVDATESDT 133
            + V S++    GLPVTF P    +  +    SLN+ F + +  C  S  WR+D +    
Sbjct: 75  LDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHS-VQL 134

Query: 134 GRRFVGIGDEDGPAGI-FGISRDNGAYNIVWCPAMMGRPRCGRAGILV-ENGVRLVALDG 193
            + FV IG + G     F I  D  AY +++CP +     C    + + ++GVR + L  
Sbjct: 135 RKSFVSIGGQKGKGNSWFQIQEDGDAYKLMYCP-ISSIVACINVSLEIDDHGVRRLVLST 194

Query: 194 D-AFPFEFIKA 196
           D +F  +F KA
Sbjct: 195 DQSFVVKFQKA 203

[Close]
BLAST of CsGy2G003800 vs. TAIR10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 70.9 bits (172), Expect = 1.0e-12
Identity = 57/187 (30.48%), Postives = 83/187 (44.39%), Query Frame = 0 

Query: 8   LAIFGYACLFMA---ITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNA 67
           L IF    +F++   +T+ A   PV D NG+ L  GV YYI P I   GG LT+ +    
Sbjct: 5   LYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE 64

Query: 68  PCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATE 127
            CP  V Q+     + GLPV F P ++    I   T +NI F   S       W +   +
Sbjct: 65  TCPTSVIQDQF-EVSQGLPVKFSPYDKS-RTIPVSTDVNIKFSPTSI------WELANFD 124

Query: 128 SDTGRRFVGIGDEDGPAG------IFGISRDNGAYNIVWCPAMMG--RPRCGRAGILVEN 184
             T + F+     +G  G       F I +    Y I +CP +    +  C   G+ V++
Sbjct: 125 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

[Close]
BLAST of CsGy2G003800 vs. Swiss-Prot
Match: sp|P13087|MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 97.4 bits (241), Expect = 1.8e-19
Identity = 77/217 (35.48%), Postives = 104/217 (47.93%), Query Frame = 0 

Query: 1   MTMLHKSLAIFGYACLFMA----ITSTAQSP--PVLDTNGQPLQRGVEYYISPAITDVGG 60
           +TML  SL+ F  + L  A    + S A S   PVLD +G+ L+ G  YYI P + D GG
Sbjct: 4   LTML--SLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGG 63

Query: 61  NLTLKSRSNAPCPLFVGQEPVTSTNIGL----PVTFRPTEEGIDIIDEGTSLNIVFQALS 120
            LT+   +  P   FV    V  T   +    P+ F P     D++   T LNI F A  
Sbjct: 64  GLTVS--ATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFM 123

Query: 121 TC--VTSTQWRVDATESDTGRRFVGIGDEDGPAGIFGISR--------DNGAYNIVWCPA 180
            C   +ST WR+D  +  TG+ FV IG   G  G   IS          +G Y +V+CP 
Sbjct: 124 PCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT 183

Query: 181 MMG--RPRCGRAGILV-ENGVRLVALDGDAFPFEFIK 195
           + G  + +CG  GI + + G R +AL    F FEF K
Sbjct: 184 VCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]
BLAST of CsGy2G003800 vs. Swiss-Prot
Match: sp|P16347|IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.7e-13
Identity = 54/143 (37.76%), Postives = 71/143 (49.65%), Query Frame = 0 

Query: 27  PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 86
           PPV DT+G  L+    YY+ PA    GG LT+       CPLFV QE       GLPV  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQE-ADGQRDGLPVRI 62

Query: 87  RP--TEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGD--EDGPAG 146
            P        II   T + I F+A +TCV ST+W +D +E  +GRR V  G   +  P+G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHID-SELVSGRRHVITGPVRDPSPSG 122

Query: 147 ---IFGISRDNGA----YNIVWC 159
               F I + +GA    Y ++ C
Sbjct: 123 RENAFRIEKYSGAEVHEYKLMAC 143

[Close]
BLAST of CsGy2G003800 vs. Swiss-Prot
Match: sp|P07596|IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 51/146 (34.93%), Postives = 69/146 (47.26%), Query Frame = 0 

Query: 24  AQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLP 83
           A  PPV DT+G  L+    YY+  A    GG LT+       CPLFV Q+P    + G P
Sbjct: 23  ADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHDGFP 82

Query: 84  VTFRP--TEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDEDGPA 143
           V   P        II   T + I F+A +TC+ ST+W +D +E   GRR V  G    P+
Sbjct: 83  VRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHID-SELAAGRRHVITGPVKDPS 142

Query: 144 -----GIFGISRDNGA----YNIVWC 159
                  F I + +GA    Y ++ C
Sbjct: 143 PSGRENAFRIEKYSGAEVHEYKLMSC 166

[Close]
BLAST of CsGy2G003800 vs. Swiss-Prot
Match: sp|P29421|IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 56/165 (33.94%), Postives = 72/165 (43.64%), Query Frame = 0 

Query: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 62
           M+   L +   + L ++ + +A  PPV DT G  L     YY+ PA    GG LT+  R 
Sbjct: 1   MVSLRLPLILLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDE----GTSLNIVFQALSTCVTSTQW 122
             PCPL V QE       G PV F P        D      T + I F A + CV ST+W
Sbjct: 61  -LPCPLLVAQE-TDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEW 120

Query: 123 RVDATESDTGRRFVGIGDEDGPA-----GIFGISRDNGAYNIVWC 159
            V   E  TG R V  G   GP+       F + +  G Y +V C
Sbjct: 121 HV-GDEPLTGARRVVTGPLIGPSPSGRENAFRVEKYGGGYKLVSC 162

[Close]
BLAST of CsGy2G003800 vs. Swiss-Prot
Match: sp|P32765|ASP_THECC (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.7e-12
Identity = 53/162 (32.72%), Postives = 76/162 (46.91%), Query Frame = 0 

Query: 17  FMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDV-GGNLTLKSRSNAPCPLFVGQEPV 76
           F  + + A S PVLDT+G  LQ GV+YY+  +I+   GG L L   +   CP  V Q   
Sbjct: 20  FFGVANAANS-PVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRS 79

Query: 77  TSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQAL--STCVTSTQWRVDATESDTGRRFV- 136
              N G PV F   +   D++   T +NI F  +    C TST WR+D  ++  G+ +V 
Sbjct: 80  DLDN-GTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVT 139

Query: 137 --GIGDEDGP-----------AGIFGISRDNGAYNIVWCPAM 162
             G+  E GP           AG+ G       Y   +CP++
Sbjct: 140 TDGVKGEPGPNTLCSWFKIEKAGVLG-------YKFRFCPSV 172

[Close]
BLAST of CsGy2G003800 vs. TrEMBL
Match: tr|A0A0A0LIK2|A0A0A0LIK2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 7.2e-98
Identity = 177/178 (99.44%), Postives = 177/178 (99.44%), Query Frame = 0 

Query: 18  MAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 77
           MAITSTAQ PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 78  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 137
           TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 138 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 196
           DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]
BLAST of CsGy2G003800 vs. TrEMBL
Match: tr|A0A0A0LGB4|A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.7e-54
Identity = 113/201 (56.22%), Postives = 135/201 (67.16%), Query Frame = 0 

Query: 1   MTMLHKSL-AIFGYACLFMAITSTAQ--SPPVLDTNGQPLQRGVEYYISPAITDVGGNLT 60
           M   HKSL A+F + C FMAI STAQ   PPVLDT G+PLQR VEY+I PAITDV GNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LKSRSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQ 120
           L +R    CP +VGQ P+ S  IG  VT  P  EG D I EG  L  VFQA + C+T TQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WRVDATESDTGRRFVGIGDEDGPAGIFGISRDN-GAYNIVWCPA---MMGRPRCGRAGIL 180
           W+V   +  +GRRFV +G+++   G F I + N G YNI WCP+     GRPRCG AGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 VENGVRLVALDGDAFPFEFIK 195
           +E GVR +ALDG AFPFEF++
Sbjct: 181 IEKGVRFLALDGPAFPFEFVR 201

[Close]
BLAST of CsGy2G003800 vs. TrEMBL
Match: tr|A0A1S3C0M7|A0A1S3C0M7_CUCME (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.0e-51
Identity = 109/202 (53.96%), Postives = 130/202 (64.36%), Query Frame = 0 

Query: 1   MTMLHKSL-AIFGYACLFMAITSTA---QSPPVLDTNGQPLQRGVEYYISPAITDVGGNL 60
           M   HKSL +IF   C FMAI STA     PPVLDT G+PLQR  EY+I PAITDV GNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLKSRSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTST 120
           TL +R    CP +VGQ P+ S   G  V+  P  EG D I EG  L  VFQ  + C+T T
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 QWRVDATESDTGRRFVGIGDEDGPAGIFGISR-DNGAYNIVWCPA---MMGRPRCGRAGI 180
           QW+V   +  TGRRFV +G ++   G F I + D G YNI WCP+   + GRPRCG AGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LVENGVRLVALDGDAFPFEFIK 195
           L+E GVR +ALDG AFPFEF++
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVR 202

[Close]
BLAST of CsGy2G003800 vs. TrEMBL
Match: tr|A0A2C9WK28|A0A2C9WK28_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G126000 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 6.0e-44
Identity = 96/197 (48.73%), Postives = 123/197 (62.44%), Query Frame = 0 

Query: 4   LHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSN 63
           + + +  F +  L M I++ AQ+P VLDT GQPL+ GVEYY+ PA TD  G LTL +R++
Sbjct: 1   MFRMIGSFSFVWLLMTISTAAQTPAVLDTTGQPLRSGVEYYVLPAATDTAGGLTLMNRTD 60

Query: 64  APCPLFVGQEPV-TSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 123
           + CPL+VGQEP+ T  + GLPV F P   G  II E   L I F A+S C  ST WRV  
Sbjct: 61  S-CPLYVGQEPLSTVVSPGLPVIFTPFSAGDGIIRESRDLRIAFSAVSICAQSTSWRVGE 120

Query: 124 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAM----MGRPRCGRAGILVENGV 183
            +++T RRF+  G   G      I ++ G Y +VWCP       GRPRC  AGIL+ENG 
Sbjct: 121 EDAETSRRFIVTG---GEQSYLRIDKNGGLYELVWCPGESCPDCGRPRCSSAGILIENGK 180

Query: 184 RLVALDGDAFPFEFIKA 196
           RL+ALDG AFPF F +A
Sbjct: 181 RLLALDGPAFPFRFRRA 193

[Close]
BLAST of CsGy2G003800 vs. TrEMBL
Match: tr|B9SIQ2|B9SIQ2_RICCO (Alpha-amylase/subtilisin inhibitor, putative OS=Ricinus communis OX=3988 GN=RCOM_0538680 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.7e-43
Identity = 93/189 (49.21%), Postives = 121/189 (64.02%), Query Frame = 0 

Query: 12  GYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVG 71
           G ACL MA+++ AQ+P VLD++GQPL  GVEYY+ PA TD  G LTL +R+ + CP +VG
Sbjct: 10  GLACLLMAVSTIAQTPAVLDSSGQPLTSGVEYYVLPAATDTAGGLTLVNRTGS-CPFYVG 69

Query: 72  QEPV-TSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRR 131
           QEP+ T    G PV F P   G  II EG    + F A++ CV ST W +   +++T RR
Sbjct: 70  QEPLPTVAKTGFPVIFTPLAAGESIIREGMDFRVAFSAVTNCVQSTTWSIGDEDAETSRR 129

Query: 132 FVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMG----RPRCGRAGILVENGVRLVALDGD 191
           F+  G E+     F I ++ G YN+ WCP        RPRCG AGIL+ENG RL+ALDG 
Sbjct: 130 FIVTGGEE---DYFRIDKNEGLYNLGWCPNCNSINCPRPRCGFAGILIENGNRLLALDGA 189

Query: 192 AFPFEFIKA 196
           AFPF+F +A
Sbjct: 190 AFPFQFRRA 194

The following BLAST results are available for this feature:
BLAST of CsGy2G003800 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004139192.14.4e-10798.97PREDICTED: miraculin-like [Cucumis sativus][more]
KGN60879.11.1e-9799.44hypothetical protein Csa_2G021500 [Cucumis sativus][more]
XP_022946294.13.8e-8276.17miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita mo... [more]
XP_023545477.14.9e-8276.68miraculin-like [Cucurbita pepo subsp. pepo][more]
XP_022999136.15.4e-8175.13miraculin-like [Cucurbita maxima][more]
BLAST of CsGy2G003800 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G73325.16.3e-1531.41Kunitz family trypsin and protease inhibitor protein[more]
AT1G17860.11.0e-1230.48Kunitz family trypsin and protease inhibitor protein[more]
BLAST of CsGy2G003800 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|P13087|MIRA_SYNDU1.8e-1935.48Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3[more]
sp|P16347|IAAS_WHEAT5.7e-1337.76Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 ... [more]
sp|P07596|IAAS_HORVU1.6e-1234.93Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2[more]
sp|P29421|IAAS_ORYSJ1.6e-1233.94Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R... [more]
sp|P32765|ASP_THECC3.7e-1232.7221 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1[more]
BLAST of CsGy2G003800 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LIK2|A0A0A0LIK2_CUCSA7.2e-9899.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=4 SV=1[more]
tr|A0A0A0LGB4|A0A0A0LGB4_CUCSA1.7e-5456.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=4 SV=1[more]
tr|A0A1S3C0M7|A0A1S3C0M7_CUCME1.0e-5153.96endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO... [more]
tr|A0A2C9WK28|A0A2C9WK28_MANES6.0e-4448.73Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G126000 PE=4 SV=... [more]
tr|B9SIQ2|B9SIQ2_RICCO1.7e-4349.21Alpha-amylase/subtilisin inhibitor, putative OS=Ricinus communis OX=3988 GN=RCOM... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR011065Kunitz_inhibitor_STI-like_sf
IPR002160Prot_inh_Kunz-lg
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G003800.1CsGy2G003800.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 28..195
e-value: 9.6E-30
score: 114.8
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 29..194
e-value: 6.3E-40
score: 136.9
IPR002160Proteinase inhibitor I3, Kunitz legumePANTHERPTHR33107FAMILY NOT NAMEDcoord: 1..194
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 29..45
IPR002160Proteinase inhibitor I3, Kunitz legumeCDDcd00178STIcoord: 28..194
e-value: 3.39716E-39
score: 132.851
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 25..195
e-value: 9.9E-44
score: 150.9
NoneNo IPR availablePANTHERPTHR33107:SF4SUBFAMILY NOT NAMEDcoord: 1..194
IPR011065Kunitz inhibitor STI-like superfamilySUPERFAMILYSSF50386STI-likecoord: 27..195

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsGy2G003800Cucurbita pepo (Zucchini)cgybcpeB168
CsGy2G003800Cucurbita maxima (Rimu)cgybcmaB178
CsGy2G003800Cucurbita maxima (Rimu)cgybcmaB257
CsGy2G003800Cucurbita moschata (Rifu)cgybcmoB170
CsGy2G003800Cucurbita moschata (Rifu)cgybcmoB243
CsGy2G003800Cucurbita pepo (Zucchini)cgybcpeB271
CsGy2G003800Cucumber (Chinese Long) v2cgybcuB056
CsGy2G003800Bottle gourd (USVL1VR-Ls)cgyblsiB089
CsGy2G003800Melon (DHL92) v3.5.1cgybmeB120
CsGy2G003800Melon (DHL92) v3.6.1cgybmedB118
CsGy2G003800Watermelon (Charleston Gray)cgybwcgB119
CsGy2G003800Watermelon (97103) v1cgybwmB140
CsGy2G003800Wild cucumber (PI 183967)cgybcpiB056
CsGy2G003800Silver-seed gourdcarcgybB0713
CsGy2G003800Silver-seed gourdcarcgybB0890
CsGy2G003800Cucumber (Chinese Long) v3cgybcucB064
CsGy2G003800Watermelon (97103) v2cgybwmbB114
CsGy2G003800Wax gourdcgybwgoB145