CsGy1G027810 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy1G027810
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	probable glucan endo-1,3-beta-glucosidase BG5
Location	Chr1 : 26162779 .. 26163360 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAGCATAATTTCCATGCGATTGCCCTATTGTTCATGGTAGCAATGTCATCTATTGTTGTGAGAGGAAACAACATTCTTGTCGGTGCATATTATGGATCTGAGGGTAACAACCTCCCACCACCATGGAAGGTGGTGCAACTCTGTGAAAAATACAACATTCGTCGCATTCGATTCAATGAACCAAATCTCGATATTCTCGAACAGTTTCGTGGCACAAATATCGAAGTCTCTTTTAGCGTACCGGGCGAGTTGATATCAAACCAGGCGACCAATCACACGGCGGTGGAGGAGTGGTTTGTCAAGTACGTCGTACCATTCATCGGCGAGTTCACCATCAACTACATCGTTGTTGGTGACAAGGTCATACCGGGACTGGACGACAACATATTGCCTGTCATGAGATCCCTTCAAGATCTCCTCAACAGTCGTTACCTCGGACAAGTGAAAATCACCACGATGGTTGGCTTAACCGCTTTGGGGGTTCAAAGCCCTCCATCTAGTGGCGCTTTTGACCCAAACGTTCTTGAGAACATGAAAGGAATCCTTAAGTTCTTATGGGACAAGGCTCGCCTTTGA

mRNA sequence

ATGGCAAAGCATAATTTCCATGCGATTGCCCTATTGTTCATGGTAGCAATGTCATCTATTGTTGTGAGAGGAAACAACATTCTTGTCGGTGCATATTATGGATCTGAGGGTAACAACCTCCCACCACCATGGAAGGTGGTGCAACTCTGTGAAAAATACAACATTCGTCGCATTCGATTCAATGAACCAAATCTCGATATTCTCGAACAGTTTCGTGGCACAAATATCGAAGTCTCTTTTAGCGTACCGGGCGAGTTGATATCAAACCAGGCGACCAATCACACGGCGGTGGAGGAGTGGTTTGTCAAGTACGTCGTACCATTCATCGGCGAGTTCACCATCAACTACATCGTTGTTGGTGACAAGGTCATACCGGGACTGGACGACAACATATTGCCTGTCATGAGATCCCTTCAAGATCTCCTCAACAGTCGTTACCTCGGACAAGTGAAAATCACCACGATGGTTGGCTTAACCGCTTTGGGGGTTCAAAGCCCTCCATCTAGTGGCGCTTTTGACCCAAACGTTCTTGAGAACATGAAAGGAATCCTTAAGTTCTTATGGGACAAGGCTCGCCTTTGA

Coding sequence (CDS)

ATGGCAAAGCATAATTTCCATGCGATTGCCCTATTGTTCATGGTAGCAATGTCATCTATTGTTGTGAGAGGAAACAACATTCTTGTCGGTGCATATTATGGATCTGAGGGTAACAACCTCCCACCACCATGGAAGGTGGTGCAACTCTGTGAAAAATACAACATTCGTCGCATTCGATTCAATGAACCAAATCTCGATATTCTCGAACAGTTTCGTGGCACAAATATCGAAGTCTCTTTTAGCGTACCGGGCGAGTTGATATCAAACCAGGCGACCAATCACACGGCGGTGGAGGAGTGGTTTGTCAAGTACGTCGTACCATTCATCGGCGAGTTCACCATCAACTACATCGTTGTTGGTGACAAGGTCATACCGGGACTGGACGACAACATATTGCCTGTCATGAGATCCCTTCAAGATCTCCTCAACAGTCGTTACCTCGGACAAGTGAAAATCACCACGATGGTTGGCTTAACCGCTTTGGGGGTTCAAAGCCCTCCATCTAGTGGCGCTTTTGACCCAAACGTTCTTGAGAACATGAAAGGAATCCTTAAGTTCTTATGGGACAAGGCTCGCCTTTGA

Protein sequence

MAKHNFHAIALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDILEQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLWDKARL

Homology

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BLAST of CsGy1G027810 vs. NCBI nr
Match: XP_004153772.2 (PREDICTED: lichenase-2-like [Cucumis sativus] >KGN63237.1 hypothetical protein Csa_2G416760 [Cucumis sativus])

HSP 1 Score: 373.2 bits (957), Expect = 5.2e-100
Identity = 184/188 (97.87%), Postives = 184/188 (97.87%), Query Frame = 0


Query: 1   MAKHNFHAIALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF 60
           MAKHNFHAIALLFMVAMSSIV RGNNI VGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF
Sbjct: 1   MAKHNFHAIALLFMVAMSSIVARGNNIFVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF 60

Query: 61  NEPNLDILEQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVG 120
           NEPNLDILEQFRGTNIEVSFSVPGELI NQATN TAVEEWFVKYVVPFIGEFTINYIVVG
Sbjct: 61  NEPNLDILEQFRGTNIEVSFSVPGELIINQATNQTAVEEWFVKYVVPFIGEFTINYIVVG 120

Query: 121 DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM 180
           DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM
Sbjct: 121 DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM 180

Query: 181 KGILKFLW 189
           KGILKFLW
Sbjct: 181 KGILKFLW 188

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BLAST of CsGy1G027810 vs. NCBI nr
Match: KGN66290.1 (hypothetical protein Csa_1G595910 [Cucumis sativus])

HSP 1 Score: 237.7 bits (605), Expect = 3.4e-59
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 72  RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI 131
           RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI
Sbjct: 7   RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI 66

Query: 132 LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLW 189
           LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLW
Sbjct: 67  LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLW 123

[Close]

BLAST of CsGy1G027810 vs. NCBI nr
Match: XP_004144115.2 (PREDICTED: lichenase-2-like [Cucumis sativus])

HSP 1 Score: 237.7 bits (605), Expect = 3.4e-59
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 72  RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI 131
           RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI
Sbjct: 7   RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI 66

Query: 132 LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLW 189
           LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLW
Sbjct: 67  LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLW 123

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BLAST of CsGy1G027810 vs. NCBI nr
Match: XP_008451156.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo])

HSP 1 Score: 235.7 bits (600), Expect = 1.3e-58
Identity = 113/188 (60.11%), Postives = 144/188 (76.60%), Query Frame = 0


Query: 1   MAKHNFHAIALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF 60
           MAK     + L+  + M + +VR +++L+GAYYG  GNNLPPPWKVV LCEKYNIRRIR 
Sbjct: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60

Query: 61  NEPNLDILEQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVG 120
           + P +D+L  FRG+NI++SFSVP +++++ ATN   VEEWF  Y+ PF+ +F INYI+VG
Sbjct: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120

Query: 121 DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGL-TALGVQSPPSSGAFDPNVLEN 180
           DK +PGLD+ ILPVM SLQDLLN+ Y GQVK+TT+VG   AL  Q PPSSG F+P V EN
Sbjct: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180

Query: 181 MKGILKFL 188
           M+GILKFL
Sbjct: 181 MRGILKFL 188

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BLAST of CsGy1G027810 vs. NCBI nr
Match: XP_022136187.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia])

HSP 1 Score: 232.3 bits (591), Expect = 1.4e-57
Identity = 110/180 (61.11%), Postives = 142/180 (78.89%), Query Frame = 0


Query: 9   IALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDIL 68
           I + FM +MS+  VR N++L+G YYG  G+N+PPPWKVVQLC KYNIRRIR +EPNL++ 
Sbjct: 7   IIVFFMASMSA--VRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEVF 66

Query: 69  EQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLD 128
           + F G  I++SF VP +++ N   N +AV EWF  YV P+IG+F INYI+VG+K IPGLD
Sbjct: 67  DAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPGLD 126

Query: 129 DNILPVMRSLQDLLNSRYLGQVKITTMVGL-TALGVQSPPSSGAFDPNVLENMKGILKFL 188
           D ILPVM+SLQ+LLN+RYLGQVK+TT+VG   AL V+ PPSSGAFDPN  +N++ IL+FL
Sbjct: 127 DCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFL 184

[Close]

BLAST of CsGy1G027810 vs. TAIR10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein)

HSP 1 Score: 114.4 bits (285), Expect = 7.9e-26
Identity = 68/184 (36.96%), Postives = 101/184 (54.89%), Query Frame = 0


Query: 6   FHAIALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNL 65
           F  +A L   +    V    NI  G  YG  G+NLPPP +V+ L +  ++  IR  +   
Sbjct: 12  FLCVAFLLNYSNVGFVDAATNI--GLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTTT 71

Query: 66  DILEQFRGT-NIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVI 125
           D+L  FRG  NI V   V  + +   + +  AV  WFV  + P++ +  I +I VG++VI
Sbjct: 72  DVLNAFRGNRNIGVMVDVKNQDLEALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEVI 131

Query: 126 PG-LDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGI 185
           PG +   +LPVM+SL +++ SR L  + I+T V +T LG   PPS+G F P   E +  +
Sbjct: 132 PGEIGSYVLPVMKSLTNIVKSRSL-PILISTTVAMTNLGQSYPPSAGDFMPQAREQLTPV 191

Query: 186 LKFL 188
           LKFL
Sbjct: 192 LKFL 192

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BLAST of CsGy1G027810 vs. TAIR10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein)

HSP 1 Score: 110.9 bits (276), Expect = 8.7e-25
Identity = 61/163 (37.42%), Postives = 93/163 (57.06%), Query Frame = 0


Query: 28  LVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDILEQFRGT-NIEVSFSVPGEL 87
           +VG  YG  G+NLP P  V++  +  N+ +IR  EPN D+L   RG  +I V+  +  E 
Sbjct: 33  VVGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFEPNKDVLNALRGNRDIGVTVGIKNED 92

Query: 88  ISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPG--LDDNILPVMRSLQDLLNS 147
           +   A N  AV+ WF   + P+I +  I +I VG++ IPG     ++LPV++SL DL+ S
Sbjct: 93  LEALAANKDAVKSWFSTNIDPYIADVNITFITVGNQAIPGDKHGPHVLPVIQSLTDLVKS 152

Query: 148 RYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFL 188
           R L Q+ I+T V  T+L    PPS+G   P   + +  +L+ L
Sbjct: 153 RNL-QISISTTVTTTSLARLKPPSAGVLTPQARQQLVPVLRLL 194

[Close]

BLAST of CsGy1G027810 vs. TAIR10
Match: AT5G20330.1 (beta-1,3-glucanase 4)

HSP 1 Score: 106.3 bits (264), Expect = 2.1e-23
Identity = 67/187 (35.83%), Postives = 103/187 (55.08%), Query Frame = 0


Query: 12  LFMVAMSSIV--VRGNNI-------LVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNE 71
           LF+  +S IV  V  NN         +G  YG  G+NLP P  V+ L +   I RIR  +
Sbjct: 8   LFLFFLSCIVLYVNSNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFD 67

Query: 72  PNLDILEQFRG-TNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGD 131
           PN ++L   RG  +IEV+  V  + ++  A +  AV+ WF   +  ++ +  I +I VG+
Sbjct: 68  PNTEVLNALRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGN 127

Query: 132 KVIPG-LDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM 188
           +VIPG +   +LPVM+SL +L+ SR L  + I+T+V ++ L    PPS+G F     E +
Sbjct: 128 EVIPGPIGPQVLPVMQSLTNLVKSRNL-PISISTVVAMSNLEQSYPPSAGMFTSQAREQL 187

[Close]

BLAST of CsGy1G027810 vs. TAIR10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein)

HSP 1 Score: 102.4 bits (254), Expect = 3.1e-22
Identity = 64/181 (35.36%), Postives = 96/181 (53.04%), Query Frame = 0


Query: 9   IALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDIL 68
           I L F    S  V   N+I  G  YG  G+N P P  V+ L +   I +IR  +P  ++L
Sbjct: 16  IMLTFNYNTSGFVAAANSI--GLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVL 75

Query: 69  EQFRG-TNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPG- 128
              RG  NIEV+  V  + +   + N  AV+ WF   + P++ +  I +I VG++VIPG 
Sbjct: 76  NALRGHRNIEVTVGVRDQDLGALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPGP 135

Query: 129 LDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKF 188
           +   +LPVM+SL  L+ S  L  + I+T+V ++ L    PPS+G F     E +  +LK 
Sbjct: 136 IGPQVLPVMQSLTILVKSMNL-PISISTVVAMSNLEQSYPPSAGEFTSQAREQLVPVLKL 193

[Close]

BLAST of CsGy1G027810 vs. TAIR10
Match: AT5G20340.1 (beta-1,3-glucanase 5)

HSP 1 Score: 102.4 bits (254), Expect = 3.1e-22
Identity = 57/157 (36.31%), Postives = 92/157 (58.60%), Query Frame = 0


Query: 33  YGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDILEQFRG-TNIEVSFSVPGELISNQA 92
           YG  G+NLP P KV+ L +  +I +IR  +PN ++L   RG  +I V+  V  + ++  +
Sbjct: 47  YGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDLAALS 106

Query: 93  TNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPG-LDDNILPVMRSLQDLLNSRYLGQV 152
            +  AV+ WF   + P++ +  I +I VG++VIPG +   +LPVM+SL +L+ SR L  +
Sbjct: 107 ASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRNL-PI 166

Query: 153 KITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFL 188
            I+T+V +  L    PPS+G F     E +  +LK L
Sbjct: 167 SISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLL 202

[Close]

BLAST of CsGy1G027810 vs. Swiss-Prot
Match: sp|P52407|E13B_HEVBR (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis OX=3981 GN=HGN1 PE=1 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 6.0e-23
Identity = 63/166 (37.95%), Postives = 92/166 (55.42%), Query Frame = 0


Query: 29  VGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDILEQFRGTNIEVSFSVPGELIS 88
           VG  YG +GNNLPP  +V+ L +K NI R+R  +PN  +LE  RG+NIE+   VP   + 
Sbjct: 38  VGVCYGMQGNNLPPVSEVIALYKKSNITRMRIYDPNRAVLEALRGSNIELILGVPNSDLQ 97

Query: 89  NQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIP------GLDDNILPVMRSLQDLL 148
           +  TN +  + W  K V  F       YI VG+++ P       L   +LP MR++ D +
Sbjct: 98  S-LTNPSNAKSWVQKNVRGFWSSVLFRYIAVGNEISPVNRGTAWLAQFVLPAMRNIHDAI 157

Query: 149 NSRYL-GQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFL 188
            S  L  Q+K++T + LT +G   PPS+GAF  +V   +  I+ FL
Sbjct: 158 RSAGLQDQIKVSTAIDLTLVGNSYPPSAGAFRDDVRSYLDPIIGFL 202

[Close]

BLAST of CsGy1G027810 vs. Swiss-Prot
Match: sp|P07979|GUB_NICPL (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 107.1 bits (266), Expect = 2.3e-22
Identity = 67/186 (36.02%), Postives = 101/186 (54.30%), Query Frame = 0


Query: 8   AIALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDI 67
           AI LL ++  S+ +V   +  VG  YG  GNNLPP  +VVQL +  NIRR+R  +PN   
Sbjct: 14  AIILLGLLVSSTEIVGAQS--VGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAA 73

Query: 68  LEQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIP-- 127
           L+  RG+NIEV   VP   + N A N +    W  + V  F       YI VG++V P  
Sbjct: 74  LQALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVT 133

Query: 128 ---GLDDNILPVMRSLQDLLNSRYL-GQVKITTMVGLTALGVQSPPSSGAFDPNVLENMK 187
               L   +LP MR++++ ++S  L   +K+++ V +T +G   PPS G+F  +V   + 
Sbjct: 134 GTSSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFID 193

[Close]

BLAST of CsGy1G027810 vs. Swiss-Prot
Match: sp|O49352|BG4_ARATH (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 3.9e-22
Identity = 67/187 (35.83%), Postives = 103/187 (55.08%), Query Frame = 0


Query: 12  LFMVAMSSIV--VRGNNI-------LVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNE 71
           LF+  +S IV  V  NN         +G  YG  G+NLP P  V+ L +   I RIR  +
Sbjct: 8   LFLFFLSCIVLYVNSNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFD 67

Query: 72  PNLDILEQFRG-TNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGD 131
           PN ++L   RG  +IEV+  V  + ++  A +  AV+ WF   +  ++ +  I +I VG+
Sbjct: 68  PNTEVLNALRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGN 127

Query: 132 KVIPG-LDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM 188
           +VIPG +   +LPVM+SL +L+ SR L  + I+T+V ++ L    PPS+G F     E +
Sbjct: 128 EVIPGPIGPQVLPVMQSLTNLVKSRNL-PISISTVVAMSNLEQSYPPSAGMFTSQAREQL 187

[Close]

BLAST of CsGy1G027810 vs. Swiss-Prot
Match: sp|O49353|BG5_ARATH (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 5.6e-21
Identity = 57/157 (36.31%), Postives = 92/157 (58.60%), Query Frame = 0


Query: 33  YGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDILEQFRG-TNIEVSFSVPGELISNQA 92
           YG  G+NLP P KV+ L +  +I +IR  +PN ++L   RG  +I V+  V  + ++  +
Sbjct: 47  YGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDLAALS 106

Query: 93  TNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPG-LDDNILPVMRSLQDLLNSRYLGQV 152
            +  AV+ WF   + P++ +  I +I VG++VIPG +   +LPVM+SL +L+ SR L  +
Sbjct: 107 ASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRNL-PI 166

Query: 153 KITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFL 188
            I+T+V +  L    PPS+G F     E +  +LK L
Sbjct: 167 SISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLL 202

[Close]

BLAST of CsGy1G027810 vs. Swiss-Prot
Match: sp|A7PQW3|E13B_VITVI (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 9.5e-21
Identity = 62/188 (32.98%), Postives = 96/188 (51.06%), Query Frame = 0


Query: 6   FHAIALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNL 65
           F  + LL  V MS+I +   +I  G  YG  GNNLP   +V+ L +   I  +R  +PN 
Sbjct: 9   FVVVLLLGFVIMSTITIGAQSI--GVCYGRNGNNLPSASQVINLYKSNGIGSMRIYDPNS 68

Query: 66  DILEQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIP 125
           D L+  RG++IE+   VP   + + A++ +A   W    VV +  E    YI VG++V+P
Sbjct: 69  DTLQALRGSDIELILDVPNTDLQSLASDASAAATWVQNNVVNYASEVKFRYIAVGNEVLP 128

Query: 126 -----GLDDNILPVMRSLQDLLNSRYL-GQVKITTMVGLTALGVQSPPSSGAFDPNVLEN 185
                     +LP M+++Q  + S  L  Q+K++T      LG   PPS G+F  +V   
Sbjct: 129 TGSNAQYAQYVLPAMKNVQSAITSAGLQDQIKVSTATFSAVLGKSYPPSEGSFSDDVSSF 188

Query: 186 MKGILKFL 188
           +  I+ FL
Sbjct: 189 INPIISFL 194

[Close]

BLAST of CsGy1G027810 vs. TrEMBL
Match: tr|A0A0A0LR03|A0A0A0LR03_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G416760 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 3.4e-100
Identity = 184/188 (97.87%), Postives = 184/188 (97.87%), Query Frame = 0


Query: 1   MAKHNFHAIALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF 60
           MAKHNFHAIALLFMVAMSSIV RGNNI VGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF
Sbjct: 1   MAKHNFHAIALLFMVAMSSIVARGNNIFVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF 60

Query: 61  NEPNLDILEQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVG 120
           NEPNLDILEQFRGTNIEVSFSVPGELI NQATN TAVEEWFVKYVVPFIGEFTINYIVVG
Sbjct: 61  NEPNLDILEQFRGTNIEVSFSVPGELIINQATNQTAVEEWFVKYVVPFIGEFTINYIVVG 120

Query: 121 DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM 180
           DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM
Sbjct: 121 DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM 180

Query: 181 KGILKFLW 189
           KGILKFLW
Sbjct: 181 KGILKFLW 188

[Close]

BLAST of CsGy1G027810 vs. TrEMBL
Match: tr|A0A0A0LWM4|A0A0A0LWM4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G595910 PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.2e-59
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 72  RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI 131
           RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI
Sbjct: 7   RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI 66

Query: 132 LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLW 189
           LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLW
Sbjct: 67  LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLW 123

[Close]

BLAST of CsGy1G027810 vs. TrEMBL
Match: tr|A0A1S3BQX5|A0A1S3BQX5_CUCME (probable glucan endo-1,3-beta-glucosidase BG5 OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 8.5e-59
Identity = 113/188 (60.11%), Postives = 144/188 (76.60%), Query Frame = 0


Query: 1   MAKHNFHAIALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF 60
           MAK     + L+  + M + +VR +++L+GAYYG  GNNLPPPWKVV LCEKYNIRRIR 
Sbjct: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60

Query: 61  NEPNLDILEQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVG 120
           + P +D+L  FRG+NI++SFSVP +++++ ATN   VEEWF  Y+ PF+ +F INYI+VG
Sbjct: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120

Query: 121 DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGL-TALGVQSPPSSGAFDPNVLEN 180
           DK +PGLD+ ILPVM SLQDLLN+ Y GQVK+TT+VG   AL  Q PPSSG F+P V EN
Sbjct: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180

Query: 181 MKGILKFL 188
           M+GILKFL
Sbjct: 181 MRGILKFL 188

[Close]

BLAST of CsGy1G027810 vs. TrEMBL
Match: tr|A0A0A0LZ10|A0A0A0LZ10_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G595900 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.0e-51
Identity = 103/180 (57.22%), Postives = 134/180 (74.44%), Query Frame = 0


Query: 9   IALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDIL 68
           + L+  + M++ VV   +IL+GAYYG  G+NLPPPWKVVQLCE+YNIRR+R + P++D+L
Sbjct: 8   VFLMASILMAATVVGAYDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVL 67

Query: 69  EQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLD 128
             FRG  I+VSF +P  ++++ A N   VEEWF  YV  F+ +F INYI+VGDK IP   
Sbjct: 68  TAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHA 127

Query: 129 DNILPVMRSLQDLLNSRYLGQVKITTMVGL-TALGVQSPPSSGAFDPNVLENMKGILKFL 188
           + ILP+M SLQDLLN+ YLGQVK+TT+VG   AL  + PPSSG FDP V E+M+GILKFL
Sbjct: 128 NFILPIMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFL 187

[Close]

BLAST of CsGy1G027810 vs. TrEMBL
Match: tr|M5VWE6|M5VWE6_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G200700 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.1e-28
Identity = 76/189 (40.21%), Postives = 112/189 (59.26%), Query Frame = 0


Query: 1   MAKHNFHAIALLFMVAM-SSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIR 60
           MA  ++ AI L F+ A  + I +   +I +G  YG  GN+LP P +VV L + Y I ++R
Sbjct: 1   MAVLHWVAIILSFVAASHNRIGLVEASIDIGVCYGMLGNDLPSPTQVVNLYKTYGIGKMR 60

Query: 61  FNEPNLDILEQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVV 120
             +PN + L+  RG+ I+VS  +  E + + A +  AV  WF  +V P++ +   N+I V
Sbjct: 61  LFDPNPEALQALRGSQIDVSIGIRNEDLPSLAVSQDAVNSWFATHVEPYLNDIVFNHITV 120

Query: 121 GDKVIPGLDDN-ILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLE 180
           G+++IPG   N +LPVM+ LQ +L+ R L  +K+TT+V   ALGV  PPSSGAF      
Sbjct: 121 GNEIIPGSSGNYVLPVMQYLQKILDDRSLAGIKVTTVVSGAALGVSYPPSSGAFTLEASS 180

Query: 181 NMKGILKFL 188
            M GIL  L
Sbjct: 181 TMSGILPLL 189

The following BLAST results are available for this feature:

BLAST of CsGy1G027810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004153772.2	5.2e-100	97.87	PREDICTED: lichenase-2-like [Cucumis sativus] >KGN63237.1 hypothetical protein C...	[more]
KGN66290.1	3.4e-59	100.00	hypothetical protein Csa_1G595910 [Cucumis sativus]	[more]
XP_004144115.2	3.4e-59	100.00	PREDICTED: lichenase-2-like [Cucumis sativus]	[more]
XP_008451156.1	1.3e-58	60.11	PREDICTED: probable glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo]	[more]
XP_022136187.1	1.4e-57	61.11	probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia]	[more]

BLAST of CsGy1G027810 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G20390.1	7.9e-26	36.96	Glycosyl hydrolase superfamily protein	[more]
AT5G20560.1	8.7e-25	37.42	Glycosyl hydrolase superfamily protein	[more]
AT5G20330.1	2.1e-23	35.83	beta-1,3-glucanase 4	[more]
AT1G33220.1	3.1e-22	35.36	Glycosyl hydrolase superfamily protein	[more]
AT5G20340.1	3.1e-22	36.31	beta-1,3-glucanase 5	[more]

BLAST of CsGy1G027810 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P52407\|E13B_HEVBR	6.0e-23	37.95	Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis O...	[more]
sp\|P07979\|GUB_NICPL	2.3e-22	36.02	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]
sp\|O49352\|BG4_ARATH	3.9e-22	35.83	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
sp\|O49353\|BG5_ARATH	5.6e-21	36.31	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
sp\|A7PQW3\|E13B_VITVI	9.5e-21	32.98	Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120...	[more]

BLAST of CsGy1G027810 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LR03\|A0A0A0LR03_CUCSA	3.4e-100	97.87	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G416760 PE=3 SV=1	[more]
tr\|A0A0A0LWM4\|A0A0A0LWM4_CUCSA	2.2e-59	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G595910 PE=3 SV=1	[more]
tr\|A0A1S3BQX5\|A0A1S3BQX5_CUCME	8.5e-59	60.11	probable glucan endo-1,3-beta-glucosidase BG5 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
tr\|A0A0A0LZ10\|A0A0A0LZ10_CUCSA	1.0e-51	57.22	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G595900 PE=3 SV=1	[more]
tr\|M5VWE6\|M5VWE6_PRUPE	2.1e-28	40.21	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G200700 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR017853	Glycoside_hydrolase_SF
IPR000490	Glyco_hydro_17

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy1G027810.1	CsGy1G027810.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.20.20.80		coord: 29..192 e-value: 4.2E-40 score: 139.6
None	No IPR available	PANTHER	PTHR32227	FAMILY NOT NAMED	coord: 24..189
None	No IPR available	PANTHER	PTHR32227:SF103	CARBOHYDRATE-BINDING X8 DOMAIN-CONTAINING PROTEIN-RELATED	coord: 24..189
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 30..189 e-value: 5.0E-26 score: 91.8
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	SSF51445	(Trans)glycosidases	coord: 29..189

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy1G027810	Cucumber (Gy14) v2	cgybcgybB011
CsGy1G027810	Cucurbita maxima (Rimu)	cgybcmaB005
CsGy1G027810	Cucurbita maxima (Rimu)	cgybcmaB027
CsGy1G027810	Cucurbita maxima (Rimu)	cgybcmaB143
CsGy1G027810	Cucurbita moschata (Rifu)	cgybcmoB006
CsGy1G027810	Cucurbita moschata (Rifu)	cgybcmoB027
CsGy1G027810	Cucurbita moschata (Rifu)	cgybcmoB134
CsGy1G027810	Cucurbita pepo (Zucchini)	cgybcpeB054
CsGy1G027810	Cucurbita pepo (Zucchini)	cgybcpeB125
CsGy1G027810	Cucumber (Chinese Long) v2	cgybcuB002
CsGy1G027810	Cucumber (Chinese Long) v2	cgybcuB019
CsGy1G027810	Cucumber (Chinese Long) v2	cgybcuB047
CsGy1G027810	Bottle gourd (USVL1VR-Ls)	cgyblsiB010
CsGy1G027810	Bottle gourd (USVL1VR-Ls)	cgyblsiB039
CsGy1G027810	Bottle gourd (USVL1VR-Ls)	cgyblsiB055
CsGy1G027810	Melon (DHL92) v3.5.1	cgybmeB036
CsGy1G027810	Melon (DHL92) v3.5.1	cgybmeB038
CsGy1G027810	Melon (DHL92) v3.5.1	cgybmeB065
CsGy1G027810	Melon (DHL92) v3.6.1	cgybmedB031
CsGy1G027810	Melon (DHL92) v3.6.1	cgybmedB032
CsGy1G027810	Melon (DHL92) v3.6.1	cgybmedB059
CsGy1G027810	Watermelon (Charleston Gray)	cgybwcgB008
CsGy1G027810	Watermelon (Charleston Gray)	cgybwcgB043
CsGy1G027810	Watermelon (97103) v1	cgybwmB002
CsGy1G027810	Watermelon (97103) v1	cgybwmB022
CsGy1G027810	Watermelon (97103) v1	cgybwmB074
CsGy1G027810	Silver-seed gourd	carcgybB0599
CsGy1G027810	Wild cucumber (PI 183967)	cgybcpiB002
CsGy1G027810	Wild cucumber (PI 183967)	cgybcpiB017
CsGy1G027810	Wild cucumber (PI 183967)	cgybcpiB045
CsGy1G027810	Silver-seed gourd	carcgybB0101
CsGy1G027810	Silver-seed gourd	carcgybB0572
CsGy1G027810	Silver-seed gourd	carcgybB0874
CsGy1G027810	Cucumber (Chinese Long) v3	cgybcucB001
CsGy1G027810	Cucumber (Chinese Long) v3	cgybcucB020
CsGy1G027810	Cucumber (Chinese Long) v3	cgybcucB052
CsGy1G027810	Watermelon (97103) v2	cgybwmbB022
CsGy1G027810	Watermelon (97103) v2	cgybwmbB041
CsGy1G027810	Watermelon (97103) v2	cgybwmbB086
CsGy1G027810	Wax gourd	cgybwgoB005
CsGy1G027810	Wax gourd	cgybwgoB073
CsGy1G027810	Wax gourd	cgybwgoB091