CsGy1G015000 (gene) Cucumber (Gy14) v2

NameCsGy1G015000
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
Descriptionsubtilisin-like protease SBT5.4
LocationChr1 : 10894006 .. 10896787 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAACTTCAATACACAAAAGTATAGACTCCATAATCCCAATTCCACAGGGAATGTAAATAAACATGTTATAGCAATTAAATTATTTATATTGTCCATTTAAGAGAATGAGATATAATTAAAGCTAAATTTGATTAAAATTCCTAGTTTTTAGTGTTTAGCAAAGGGTTTCTTTATAAAGAGAAGGAAGCAGAAAAGAAAGTGATTGATTGTTAGGTGTTTTTTTGGAGATCATGAAGCATCGGAAGATGAAGATCTATAATTCCATTCTCATTTTATTTTCACTCCTTTTGATAATATCACCTGCCATGGCTGCCAAAAAGGTAATCAAAATATTAGAAAAGAGATTGTTTTTAAACAAGTCTTGAAGTTGTTTTATTATGCATGCATTATCTAATATTTATACCCATTTTGTTCTTTACTATTAAAAAAGATATATTGATTTGGTTTGGTTCATTAGATTGTTTTTTGGGTTGTGATTTTTTTCTTCCCAAAGCCCTAAAAGTTAAAATTGTAGTTTTCCTGATCTTTATTATTTGTTTTTTTTTTTATACATTGATTGATTCAGTCTATACTTTCTATGTTCATAAAGACATTATTAAACAAACCCCAAAACCCCAAAAATGGTTGGGACAAATTTCCTTTTCTTACTATATATGTGTATATAATTATCGATTAGGTTTGGTCAATATGAAGTGTTCTTGGGTTTTGAATTTTTTTTCTTCAGGTTTACAAAACCTCTTTTCTTTTTTTGGTTCTTGAACTCAGTTGATTCAGTGGTGTACTTAGTTTGTATGTATCTATGAATTATTAAAAATTATCTTAAAGATTGGATAGCAAAAACAATTAGCTCTATTTTGTAAAATAGAGCAAAATCTACTAAATTCTTTAGGTTCATTTAAGTTTTTTTTTTTTTTTTTTTTTGCATAATATAGTTTCAATTTTTGTTTATTTTGCTATCAATAAGAGTTTCTCCAAAACTAAATGGTTTTTTTTTTGGTTTAATGTTTGGATTTCAGTCTTATATTGTGTTATTGGGATCCCATTCACATGGATTAGAAGTTACAGATGAAGATCTTAAAAGGGTGGCTGATTCCCATCACAAGTTGCTTGGATCCATCTTTGGAAGGTTTGAAATTTTATCTATAATTTCCTCATCATAACAACTTATTTTTCATTTGATTACTTTTTTTTTTAGAAAAAAATCTTGTTGTTGTTATTATTATAATTCTTACTTATTAAATTTTAATTATTATAAAATTTGAAAATTTCAGTGATGAGAAGGCAAGAAATGCTATATTTTACTCCTATAAGAAGAATATAAATGGTTTTGCAGCAATTATGGACGAGGAAGAGGCAGCCCAGCTTGCAAGTAAAAAAATCAACTCATATACTTCTTTTAAAAAGAAATCTATTTTCACATCTTTTAGCATAACCAAGGTTTAATTTAAAAAACAAAAAGAATTATAATAATAGAAACTAAATTGGAAAATTATAAAAAATGTGACAGAACACCCGGAAGTAGCAGCAGTATTGCCAAACAGAGCAAAAAAGTTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCCCTTCATCTGCTTGGCGTAGGGCTAAGTCTGGAAAAGACGTCATTATCGCCAATCTTGACACTGGTACACTTCTTTTTTTCTATTTTAATTAATATAGCAAAGATTTTATTCTTAGTATTTTACAAATTTAATTAATATAGCATAAATGATTGGAACAAATATGAAAACATAGGATAAAGACTTTGATGGAAGTAAAGTTTAGTTTATGGTCCAAATTAAAATAAATGTATATATTTGTTATCTTTTCTGTTTTGGGAAAAGGTGTGTGGCCGGAGTCCAAGAGTTTTGGAGAACATGGGATAGTTGGACCTGTTCCATCAAAGTGGAAAGGAGGTTGCACTGATAAAACCCTTGATAGAGTGCCATGTAACAGGTTTGATGTTTTTGACACGAAGAGTTAATTAACTACTTTGATATTGATATTGATATTGATTTAAGGTTGATTATTTTTCTTTAATATTAGATTGATTTTAAAATAAATAAATATAGGAAACTAATAGGAGCAAAGTACTTCAACAAGGGCTTCCTCGCATACTTGAAGTCTGAGAATTTGACAGCATTGGTGATTAACTCCACTCGTGACTACGACGGTCATGGTTCACACACCTTGTCCACGGCTGGGGGCAGCTATGTGTCTGGCGCCAGTGTGTTCGGGTTAGGCGTTGGAACTGCCAAAGGGGGCTCTCCCAAGGCACGTGTTGCTGCCTATAAGGTTTGCTGGCCACTTGAGGATGGAGGTTGTTTCGACGCTGACATTGCCCAGGCATTTGACCATGCCATTCACGACCGCGTTGATGTTCTTTCGCTCTCCCTTGGTGGCGAGCCAGCCGATTATTATGATGATGGAATCGCCATTTCAGCATTCCATGCAGTGAAGAAGGGAATCCCTGTGGTGTGCTCGGCTGGTAACTCTGGCCCTGGTGCTCAGACTGTTTCTAATACTGCACCTTGGATTTTGACTGTTGGTGCAAGTACTATGGATCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGTCACCGTTATATGGTTCGTCTCAGCTTTCACTCTCTAATTGAATGTGTGTATGAGCTCTATTTATATTTTGTTGGTTTTCTATATTCATTAGGGTTCGAGCCTTTCAAAAGGATTAAAGGGAGACAAGCTTTATCCATTAATAACTGGAGCTGA

mRNA sequence

AAAAACTTCAATACACAAAAGTATAGACTCCATAATCCCAATTCCACAGGGAATGTAAATAAACATGTTATAGCAATTAAATTATTTATATTGTCCATTTAAGAGAATGAGATATAATTAAAGCTAAATTTGATTAAAATTCCTAGTTTTTAGTGTTTAGCAAAGGGTTTCTTTATAAAGAGAAGGAAGCAGAAAAGAAAGTGATTGATTGTTAGGTGTTTTTTTGGAGATCATGAAGCATCGGAAGATGAAGATCTATAATTCCATTCTCATTTTATTTTCACTCCTTTTGATAATATCACCTGCCATGGCTGCCAAAAAGGCAAGAAATGCTATATTTTACTCCTATAAGAAGAATATAAATGGTTTTGCAGCAATTATGGACGAGGAAGAGGCAGCCCAGCTTGCAAAACACCCGGAAGTAGCAGCAGTATTGCCAAACAGAGCAAAAAAGTTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCCCTTCATCTGCTTGGCGTAGGGCTAAGTCTGGAAAAGACGTCATTATCGCCAATCTTGACACTGGTGTGTGGCCGGAGTCCAAGAGTTTTGGAGAACATGGGATAGTTGGACCTGTTCCATCAAAGTGGAAAGGAGGTTGCACTGATAAAACCCTTGATAGAGTGCCATGTAACAGGAAACTAATAGGAGCAAAGTACTTCAACAAGGGCTTCCTCGCATACTTGAAGTCTGAGAATTTGACAGCATTGGTGATTAACTCCACTCGTGACTACGACGGTCATGGTTCACACACCTTGTCCACGGCTGGGGGCAGCTATGTGTCTGGCGCCAGTGTGTTCGGGTTAGGCGTTGGAACTGCCAAAGGGGGCTCTCCCAAGGCACGTGTTGCTGCCTATAAGGTTTGCTGGCCACTTGAGGATGGAGGTTGTTTCGACGCTGACATTGCCCAGGCATTTGACCATGCCATTCACGACCGCGTTGATGTTCTTTCGCTCTCCCTTGGTGGCGAGCCAGCCGATTATTATGATGATGGAATCGCCATTTCAGCATTCCATGCAGTGAAGAAGGGAATCCCTGTGGTGTGCTCGGCTGGTAACTCTGGCCCTGGTGCTCAGACTGTTTCTAATACTGCACCTTGGATTTTGACTGTTGGTGCAAGTACTATGGATCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGTCACCGTTATATGGTTCGTCTCAGCTTTCACTCTCTAATTGAATGTGTGTATGAGCTCTATTTATATTTTGTTGGTTTTCTATATTCATTAGGGTTCGAGCCTTTCAAAAGGATTAAAGGGAGACAAGCTTTATCCATTAATAACTGGAGCTGA

Coding sequence (CDS)

ATGAAGCATCGGAAGATGAAGATCTATAATTCCATTCTCATTTTATTTTCACTCCTTTTGATAATATCACCTGCCATGGCTGCCAAAAAGGCAAGAAATGCTATATTTTACTCCTATAAGAAGAATATAAATGGTTTTGCAGCAATTATGGACGAGGAAGAGGCAGCCCAGCTTGCAAAACACCCGGAAGTAGCAGCAGTATTGCCAAACAGAGCAAAAAAGTTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCCCTTCATCTGCTTGGCGTAGGGCTAAGTCTGGAAAAGACGTCATTATCGCCAATCTTGACACTGGTGTGTGGCCGGAGTCCAAGAGTTTTGGAGAACATGGGATAGTTGGACCTGTTCCATCAAAGTGGAAAGGAGGTTGCACTGATAAAACCCTTGATAGAGTGCCATGTAACAGGAAACTAATAGGAGCAAAGTACTTCAACAAGGGCTTCCTCGCATACTTGAAGTCTGAGAATTTGACAGCATTGGTGATTAACTCCACTCGTGACTACGACGGTCATGGTTCACACACCTTGTCCACGGCTGGGGGCAGCTATGTGTCTGGCGCCAGTGTGTTCGGGTTAGGCGTTGGAACTGCCAAAGGGGGCTCTCCCAAGGCACGTGTTGCTGCCTATAAGGTTTGCTGGCCACTTGAGGATGGAGGTTGTTTCGACGCTGACATTGCCCAGGCATTTGACCATGCCATTCACGACCGCGTTGATGTTCTTTCGCTCTCCCTTGGTGGCGAGCCAGCCGATTATTATGATGATGGAATCGCCATTTCAGCATTCCATGCAGTGAAGAAGGGAATCCCTGTGGTGTGCTCGGCTGGTAACTCTGGCCCTGGTGCTCAGACTGTTTCTAATACTGCACCTTGGATTTTGACTGTTGGTGCAAGTACTATGGATCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGTCACCGTTATATGGTTCGTCTCAGCTTTCACTCTCTAATTGAATGTGTGTATGAGCTCTATTTATATTTTGTTGGTTTTCTATATTCATTAGGGTTCGAGCCTTTCAAAAGGATTAAAGGGAGACAAGCTTTATCCATTAATAACTGGAGCTGA

Protein sequence

MKHRKMKIYNSILILFSLLLIISPAMAAKKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMVRLSFHSLIECVYELYLYFVGFLYSLGFEPFKRIKGRQALSINNWS
BLAST of CsGy1G015000 vs. NCBI nr
Match: XP_004139597.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 625.9 bits (1613), Expect = 8.7e-176
Identity = 303/304 (99.67%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           +KARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK
Sbjct: 44  EKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 103

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 148
           NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV
Sbjct: 104 NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 163

Query: 149 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 208
           PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL
Sbjct: 164 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 223

Query: 209 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY 268
           GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY
Sbjct: 224 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY 283

Query: 269 DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG 328
           DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG
Sbjct: 284 DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG 343

Query: 329 HRYM 333
           HRYM
Sbjct: 344 HRYM 347

BLAST of CsGy1G015000 vs. NCBI nr
Match: KGN64982.1 (hypothetical protein Csa_1G171040 [Cucumis sativus])

HSP 1 Score: 625.9 bits (1613), Expect = 8.7e-176
Identity = 303/304 (99.67%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           +KARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK
Sbjct: 50  EKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 109

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 148
           NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV
Sbjct: 110 NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 169

Query: 149 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 208
           PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL
Sbjct: 170 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 229

Query: 209 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY 268
           GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY
Sbjct: 230 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY 289

Query: 269 DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG 328
           DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG
Sbjct: 290 DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG 349

Query: 329 HRYM 333
           HRYM
Sbjct: 350 HRYM 353

BLAST of CsGy1G015000 vs. NCBI nr
Match: XP_008464322.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo])

HSP 1 Score: 609.4 bits (1570), Expect = 8.4e-171
Identity = 308/372 (82.80%), Postives = 319/372 (85.75%), Query Frame = 0

Query: 1   MKHRKMKIYNSILILFSLLLIISPAMAAK------------------------------- 60
           MKH KMKIYNSILILFSLLL+ISPA+AAK                               
Sbjct: 1   MKHPKMKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKL 60

Query: 61  ---------KARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHS 120
                    KAR+AIFYSYK+NINGFAAIMDEEEA QLAKHPEVAAVL N+AKKLHTTHS
Sbjct: 61  LGSIIGSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHS 120

Query: 121 WEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 180
           WEFMHLEKNGVIPPSSAWRRAKSGKDVII NLDTGVW ESKSFGEHGIVG VPSKWKGGC
Sbjct: 121 WEFMHLEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGC 180

Query: 181 TDKTLDRVPCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYV 240
           TDKT D V CNRKLIGAKYFNKGFLAYL S+NLTA VINSTRDYDGHGSHTLSTAGGSYV
Sbjct: 181 TDKTPDGVSCNRKLIGAKYFNKGFLAYLNSQNLTASVINSTRDYDGHGSHTLSTAGGSYV 240

Query: 241 SGASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSL 300
           SGASVFGLGVGTAKGGSPKARVA+YKVCWPLEDGGCF+ADIA+AFDHAIHDRVDVLSLSL
Sbjct: 241 SGASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSL 300

Query: 301 GGEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQ 333
           GGEPADYYDDGIAI+AFHAVKKGIPVVCSAGNSGP AQTVSNTAPWILTVGAST+DREFQ
Sbjct: 301 GGEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQ 360

BLAST of CsGy1G015000 vs. NCBI nr
Match: XP_023520266.1 (subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523.9 bits (1348), Expect = 4.7e-145
Identity = 251/306 (82.03%), Postives = 277/306 (90.52%), Query Frame = 0

Query: 30  KARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKN 89
           KA++AIFYSYK +INGFAAI+DEEEA +LAKHPEVAAVLPN+AK+LHTT SWEFMHLEKN
Sbjct: 65  KAKDAIFYSYKNHINGFAAILDEEEATKLAKHPEVAAVLPNKAKELHTTRSWEFMHLEKN 124

Query: 90  GVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVP 149
           GV PPSS WR+AKSG++VIIANLDTGVWPESKSFGEHGIVG VPS+WKGGCTDKT D VP
Sbjct: 125 GVSPPSSPWRKAKSGRNVIIANLDTGVWPESKSFGEHGIVGGVPSRWKGGCTDKTPDAVP 184

Query: 150 CNRKLIGAKYFNKGFLAYLKSENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGASVF 209
           CNRKLIGAKYFN G +AYLKSENLT   + ++NSTRDY+GHGSHTLSTAGGSYVSG SVF
Sbjct: 185 CNRKLIGAKYFNHGVIAYLKSENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVSGVSVF 244

Query: 210 GLGVGTAKGGSPKARVAAYKVCWPLED-GGCFDADIAQAFDHAIHDRVDVLSLSLGGEPA 269
           G G+GTAKGGSPKA VAAYKVCWPL D GGCFDADIA+AFDHAIHD VDVLSLSLG  PA
Sbjct: 245 GSGIGTAKGGSPKAHVAAYKVCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSLSLGSPPA 304

Query: 270 DYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVEL 329
           +YYDD IAI++FHA+KKGIPVVCSAGN GP   TVSNTAPWILTVGAST+DREFQAP+EL
Sbjct: 305 EYYDDIIAIASFHALKKGIPVVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQAPIEL 364

Query: 330 QNGHRY 332
           +NG  +
Sbjct: 365 RNGKHF 370

BLAST of CsGy1G015000 vs. NCBI nr
Match: XP_022927096.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 517.7 bits (1332), Expect = 3.3e-143
Identity = 246/309 (79.61%), Postives = 279/309 (90.29%), Query Frame = 0

Query: 27  AAKKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHL 86
           +++KA++AIFYSYK +INGFAAI+DEEEA +LAKHPE+AAVLPN+AK+LHTT SWEFMHL
Sbjct: 40  SSEKAKDAIFYSYKNHINGFAAILDEEEATKLAKHPEIAAVLPNKAKELHTTRSWEFMHL 99

Query: 87  EKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLD 146
           EKNGV PPSS WR+A+SG++VIIANLDTGVWPESKSFGEHGIVG VPSKWKGGC DKT D
Sbjct: 100 EKNGVSPPSSPWRKARSGQNVIIANLDTGVWPESKSFGEHGIVGGVPSKWKGGCMDKTPD 159

Query: 147 RVPCNRKLIGAKYFNKGFLAYLKSENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGA 206
            VPCN+KLIGAKYFN G +AYLKSENLT   + ++NSTRDY+GHGSHTLSTAGGSYVSG 
Sbjct: 160 AVPCNKKLIGAKYFNHGVIAYLKSENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVSGV 219

Query: 207 SVFGLGVGTAKGGSPKARVAAYKVCWPLED-GGCFDADIAQAFDHAIHDRVDVLSLSLGG 266
           SVFG G+GTAKGGSPKA VAAYKVCWPL D GGCFDADIA+AFDHAIHD VDVLS+SLG 
Sbjct: 220 SVFGSGIGTAKGGSPKAHVAAYKVCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSVSLGS 279

Query: 267 EPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAP 326
            PA+YY+D IAI++FHA+KKGIPVVCSAGN GP   TVSNTAPWILTVGAST+DREFQAP
Sbjct: 280 PPAEYYNDIIAIASFHALKKGIPVVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQAP 339

Query: 327 VELQNGHRY 332
           +EL+NG  +
Sbjct: 340 IELRNGKHF 348

BLAST of CsGy1G015000 vs. TAIR10
Match: AT5G59810.1 (Subtilase family protein)

HSP 1 Score: 401.4 bits (1030), Expect = 6.3e-112
Identity = 201/308 (65.26%), Postives = 238/308 (77.27%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           + A+ AIFYSYK++INGFAAI+DE EAA++AKHP+V +V PN+ +KLHTTHSW FM L K
Sbjct: 79  ENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAK 138

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 148
           NGV+  SS W +A  G+D IIANLDTGVWPESKSF + G  G VP++WKG C       V
Sbjct: 139 NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD----V 198

Query: 149 PCNRKLIGAKYFNKGFLAYLKSENLTALVINST----RDYDGHGSHTLSTAGGSYVSGAS 208
           PCNRKLIGA+YFNKG+LAY      T L  N++    RD+DGHGSHTLSTA G++V GA+
Sbjct: 199 PCNRKLIGARYFNKGYLAY------TGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGAN 258

Query: 209 VFGLGVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAQAFDHAIHDRVDVLSLSLGGE 268
           VFG+G GTA GGSPKARVAAYKVCWP  DG  CFDADI  A + AI D VDVLS S+GG+
Sbjct: 259 VFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGD 318

Query: 269 PADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPV 328
             DY  DGIAI +FHAVK G+ VVCSAGNSGP + TVSN APW++TVGAS+MDREFQA V
Sbjct: 319 AGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFV 375

Query: 329 ELQNGHRY 332
           EL+NG  +
Sbjct: 379 ELKNGQSF 375

BLAST of CsGy1G015000 vs. TAIR10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 381.3 bits (978), Expect = 6.8e-106
Identity = 186/304 (61.18%), Postives = 229/304 (75.33%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           ++A +AIFYSY K+INGFAA +D + A +++KHPEV +V PN+A KLHTT SW+F+ LE 
Sbjct: 69  ERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEH 128

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 148
           N  +P SS WR+A+ G+D IIANLDTGVWPESKSF + G+ GP+PS+WKG C ++     
Sbjct: 129 NSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDATF 188

Query: 149 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 208
            CNRKLIGA+YFNKG+ A +   N +    +S RD DGHGSHTLSTA G +V G S+FG 
Sbjct: 189 HCNRKLIGARYFNKGYAAAVGHLNSS---FDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQ 248

Query: 209 GVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAQAFDHAIHDRVDVLSLSLGGEPADY 268
           G GTAKGGSP+ARVAAYKVCWP   G  C+DAD+  AFD AIHD  DV+S+SLGGEP  +
Sbjct: 249 GNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF 308

Query: 269 YDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQN 328
           ++D +AI +FHA KK I VVCSAGNSGP   TVSN APW +TVGASTMDREF + + L N
Sbjct: 309 FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGN 368

Query: 329 GHRY 332
           G  Y
Sbjct: 369 GKHY 368

BLAST of CsGy1G015000 vs. TAIR10
Match: AT4G10550.3 (Subtilase family protein)

HSP 1 Score: 278.1 bits (710), Expect = 8.1e-75
Identity = 150/306 (49.02%), Postives = 194/306 (63.40%), Query Frame = 0

Query: 31  ARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNG 90
           A +++ YSY+   +GFAA + E +A ++A  P+V  V+P+   KL TT +W+++ L    
Sbjct: 84  ANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGL---S 143

Query: 91  VIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC-TDKTLDRVP 150
              P S       G+ +II  +DTGVWPES+ F + G  GPVPS WKGGC T +  +   
Sbjct: 144 AANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGF-GPVPSHWKGGCETGENFNSSN 203

Query: 151 CNRKLIGAKYFNKGFLAYLKSENLT-ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 210
           CN+KLIGAKYF  GFLA  +S N T +L   S RD DGHG+H  + AGGS+V   S  GL
Sbjct: 204 CNKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGL 263

Query: 211 GVGTAKGGSPKARVAAYKVCWPLEDGG---CFDADIAQAFDHAIHDRVDVLSLSLGGEPA 270
             GT +GG+P+A +A YK CW L+D     C  ADI +A D A+HD VDVLS+SLG    
Sbjct: 264 AGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVP 323

Query: 271 DYYD----DGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQA 328
            Y +    DGI   AFHAV KGI VVCS GNSGP + TV+NTAPWI+TV A+T+DR F  
Sbjct: 324 LYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFAT 383

BLAST of CsGy1G015000 vs. TAIR10
Match: AT4G10510.1 (Subtilase family protein)

HSP 1 Score: 276.2 bits (705), Expect = 3.1e-74
Identity = 150/306 (49.02%), Postives = 198/306 (64.71%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           ++A  ++ +S++   +GFAA + E +A ++A  PEV  V+P+R  K  TT +W+++ L  
Sbjct: 54  EEAHGSMVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRTWDYLGLSP 113

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC-TDKTLDR 148
                P +   +   G+ +II  +D+GVWPES+ F ++ I GPVPS WKGGC + +  + 
Sbjct: 114 TN---PKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNEI-GPVPSHWKGGCESGEDFNS 173

Query: 149 VPCNRKLIGAKYFNKGFLAYLKSENLT-ALVINSTRDYDGHGSHTLSTAGGSYVSGASVF 208
             CN+KLIGAKYF   FLA  +S N + +L   S R Y+GHG+H  + AGGSYV   S  
Sbjct: 174 SHCNKKLIGAKYFINAFLATHESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNTSYK 233

Query: 209 GLGVGTAKGGSPKARVAAYKVCW--PLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGE- 268
           GL  GT +GG+P+AR+A YK CW   L+   C  ADI +A D AIHD VDVLSLSLG E 
Sbjct: 234 GLAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSLGFEP 293

Query: 269 --PADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQA 328
             P     DGIA  AFHAV KGI VVC+AGN+GP AQTV NTAPWILTV A+T+DR F  
Sbjct: 294 LYPETDVRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDRSFVT 353

BLAST of CsGy1G015000 vs. TAIR10
Match: AT1G32960.1 (Subtilase family protein)

HSP 1 Score: 274.2 bits (700), Expect = 1.2e-73
Identity = 151/309 (48.87%), Postives = 193/309 (62.46%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           K A +++ YSY+   +GFAA + + +A ++A  PEV  V+P+   +L TT +WE++ L  
Sbjct: 64  KDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGLSS 123

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC-TDKTLDR 148
                P +       G  VII  +DTGVWPES+SF ++G VGP+P KWKGGC + +    
Sbjct: 124 AN---PKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNG-VGPIPRKWKGGCESGENFRS 183

Query: 149 VPCNRKLIGAKYFNKGFLAYLKSENLT-ALVINSTRDYDGHGSHTLSTAGGSYVSGASVF 208
             CNRKLIGAKYF  GFLA  K  N T +    S RD+DGHG+H  S AGGS+V   S  
Sbjct: 184 TDCNRKLIGAKYFINGFLAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYK 243

Query: 209 GLGVGTAKGGSPKARVAAYKVCW---PLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGE 268
           GL  GT +GG+P+AR+A YK CW    L+   C D+DI +A D AIHD VDVLS+SL G+
Sbjct: 244 GLAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQ 303

Query: 269 -----PADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDRE 328
                  D  D+  A   FHAV KGI VVC+ GN GP AQTV N APWILTV A+T+DR 
Sbjct: 304 IPLNSETDIRDE-FATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRS 363

BLAST of CsGy1G015000 vs. Swiss-Prot
Match: sp|F4JXC5|SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-110
Identity = 201/308 (65.26%), Postives = 238/308 (77.27%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           + A+ AIFYSYK++INGFAAI+DE EAA++AKHP+V +V PN+ +KLHTTHSW FM L K
Sbjct: 79  ENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAK 138

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 148
           NGV+  SS W +A  G+D IIANLDTGVWPESKSF + G  G VP++WKG C       V
Sbjct: 139 NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD----V 198

Query: 149 PCNRKLIGAKYFNKGFLAYLKSENLTALVINST----RDYDGHGSHTLSTAGGSYVSGAS 208
           PCNRKLIGA+YFNKG+LAY      T L  N++    RD+DGHGSHTLSTA G++V GA+
Sbjct: 199 PCNRKLIGARYFNKGYLAY------TGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGAN 258

Query: 209 VFGLGVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAQAFDHAIHDRVDVLSLSLGGE 268
           VFG+G GTA GGSPKARVAAYKVCWP  DG  CFDADI  A + AI D VDVLS S+GG+
Sbjct: 259 VFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGD 318

Query: 269 PADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPV 328
             DY  DGIAI +FHAVK G+ VVCSAGNSGP + TVSN APW++TVGAS+MDREFQA V
Sbjct: 319 AGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFV 375

Query: 329 ELQNGHRY 332
           EL+NG  +
Sbjct: 379 ELKNGQSF 375

BLAST of CsGy1G015000 vs. Swiss-Prot
Match: sp|Q9ZSP5|AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.2e-104
Identity = 186/304 (61.18%), Postives = 229/304 (75.33%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           ++A +AIFYSY K+INGFAA +D + A +++KHPEV +V PN+A KLHTT SW+F+ LE 
Sbjct: 69  ERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEH 128

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 148
           N  +P SS WR+A+ G+D IIANLDTGVWPESKSF + G+ GP+PS+WKG C ++     
Sbjct: 129 NSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDATF 188

Query: 149 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 208
            CNRKLIGA+YFNKG+ A +   N +    +S RD DGHGSHTLSTA G +V G S+FG 
Sbjct: 189 HCNRKLIGARYFNKGYAAAVGHLNSS---FDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQ 248

Query: 209 GVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAQAFDHAIHDRVDVLSLSLGGEPADY 268
           G GTAKGGSP+ARVAAYKVCWP   G  C+DAD+  AFD AIHD  DV+S+SLGGEP  +
Sbjct: 249 GNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF 308

Query: 269 YDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQN 328
           ++D +AI +FHA KK I VVCSAGNSGP   TVSN APW +TVGASTMDREF + + L N
Sbjct: 309 FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGN 368

Query: 329 GHRY 332
           G  Y
Sbjct: 369 GKHY 368

BLAST of CsGy1G015000 vs. Swiss-Prot
Match: sp|I1N462|SBT1_SOYBN (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 306.2 bits (783), Expect = 5.0e-82
Identity = 158/310 (50.97%), Postives = 216/310 (69.68%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           +KA+ AI YSY ++INGFAA+++EEEAA +AK+P V +V  ++  KLHTT SWEF+ L +
Sbjct: 69  EKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHR 128

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 148
            G    +SAW++ + G++ II N+DTGVWPES+SF + G  G VPSKW+GG     ++++
Sbjct: 129 RG---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKG-YGTVPSKWRGGLCQ--INKL 188

Query: 149 P------CNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSG 208
           P      CNRKLIGA+Y+NK F A+  +  L  L +++ RD+ GHG+HTLSTAGG++V G
Sbjct: 189 PGSMKNTCNRKLIGARYYNKAFEAH--NGQLDPL-LHTARDFVGHGTHTLSTAGGNFVPG 248

Query: 209 ASVFGLGVGTAKGGSPKARVAAYKVCWPLED-GGCFDADIAQAFDHAIHDRVDVLSLSLG 268
           A VF +G GTAKGGSP+ARVAAYKVCW L D   C+ AD+  A D AI D VDV+++S G
Sbjct: 249 ARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFG 308

Query: 269 GE----PADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDR 328
                     + D I+I AFHA+ K I +V SAGN GP   TV+N APW+ T+ AST+DR
Sbjct: 309 VSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDR 368

BLAST of CsGy1G015000 vs. Swiss-Prot
Match: sp|Q8L7I2|SBT36_ARATH (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.5e-73
Identity = 150/306 (49.02%), Postives = 194/306 (63.40%), Query Frame = 0

Query: 31  ARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNG 90
           A +++ YSY+   +GFAA + E +A ++A  P+V  V+P+   KL TT +W+++ L    
Sbjct: 68  ANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGL---S 127

Query: 91  VIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC-TDKTLDRVP 150
              P S       G+ +II  +DTGVWPES+ F + G  GPVPS WKGGC T +  +   
Sbjct: 128 AANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGF-GPVPSHWKGGCETGENFNSSN 187

Query: 151 CNRKLIGAKYFNKGFLAYLKSENLT-ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 210
           CN+KLIGAKYF  GFLA  +S N T +L   S RD DGHG+H  + AGGS+V   S  GL
Sbjct: 188 CNKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGL 247

Query: 211 GVGTAKGGSPKARVAAYKVCWPLEDGG---CFDADIAQAFDHAIHDRVDVLSLSLGGEPA 270
             GT +GG+P+A +A YK CW L+D     C  ADI +A D A+HD VDVLS+SLG    
Sbjct: 248 AGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVP 307

Query: 271 DYYD----DGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQA 328
            Y +    DGI   AFHAV KGI VVCS GNSGP + TV+NTAPWI+TV A+T+DR F  
Sbjct: 308 LYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFAT 367

BLAST of CsGy1G015000 vs. Swiss-Prot
Match: sp|Q9SZY2|SBT37_ARATH (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 276.2 bits (705), Expect = 5.5e-73
Identity = 150/306 (49.02%), Postives = 198/306 (64.71%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           ++A  ++ +S++   +GFAA + E +A ++A  PEV  V+P+R  K  TT +W+++ L  
Sbjct: 66  EEAHGSMVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRTWDYLGLSP 125

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC-TDKTLDR 148
                P +   +   G+ +II  +D+GVWPES+ F ++ I GPVPS WKGGC + +  + 
Sbjct: 126 TN---PKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNEI-GPVPSHWKGGCESGEDFNS 185

Query: 149 VPCNRKLIGAKYFNKGFLAYLKSENLT-ALVINSTRDYDGHGSHTLSTAGGSYVSGASVF 208
             CN+KLIGAKYF   FLA  +S N + +L   S R Y+GHG+H  + AGGSYV   S  
Sbjct: 186 SHCNKKLIGAKYFINAFLATHESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNTSYK 245

Query: 209 GLGVGTAKGGSPKARVAAYKVCW--PLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGE- 268
           GL  GT +GG+P+AR+A YK CW   L+   C  ADI +A D AIHD VDVLSLSLG E 
Sbjct: 246 GLAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSLGFEP 305

Query: 269 --PADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQA 328
             P     DGIA  AFHAV KGI VVC+AGN+GP AQTV NTAPWILTV A+T+DR F  
Sbjct: 306 LYPETDVRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDRSFVT 365

BLAST of CsGy1G015000 vs. TrEMBL
Match: tr|A0A0A0LVY8|A0A0A0LVY8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=4 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 5.8e-176
Identity = 303/304 (99.67%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 29  KKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 88
           +KARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK
Sbjct: 50  EKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK 109

Query: 89  NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 148
           NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV
Sbjct: 110 NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRV 169

Query: 149 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 208
           PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL
Sbjct: 170 PCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL 229

Query: 209 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY 268
           GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY
Sbjct: 230 GVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYY 289

Query: 269 DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG 328
           DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG
Sbjct: 290 DDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNG 349

Query: 329 HRYM 333
           HRYM
Sbjct: 350 HRYM 353

BLAST of CsGy1G015000 vs. TrEMBL
Match: tr|A0A1S3CLB7|A0A1S3CLB7_CUCME (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 5.6e-171
Identity = 308/372 (82.80%), Postives = 319/372 (85.75%), Query Frame = 0

Query: 1   MKHRKMKIYNSILILFSLLLIISPAMAAK------------------------------- 60
           MKH KMKIYNSILILFSLLL+ISPA+AAK                               
Sbjct: 1   MKHPKMKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKL 60

Query: 61  ---------KARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHS 120
                    KAR+AIFYSYK+NINGFAAIMDEEEA QLAKHPEVAAVL N+AKKLHTTHS
Sbjct: 61  LGSIIGSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHS 120

Query: 121 WEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 180
           WEFMHLEKNGVIPPSSAWRRAKSGKDVII NLDTGVW ESKSFGEHGIVG VPSKWKGGC
Sbjct: 121 WEFMHLEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGC 180

Query: 181 TDKTLDRVPCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYV 240
           TDKT D V CNRKLIGAKYFNKGFLAYL S+NLTA VINSTRDYDGHGSHTLSTAGGSYV
Sbjct: 181 TDKTPDGVSCNRKLIGAKYFNKGFLAYLNSQNLTASVINSTRDYDGHGSHTLSTAGGSYV 240

Query: 241 SGASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSL 300
           SGASVFGLGVGTAKGGSPKARVA+YKVCWPLEDGGCF+ADIA+AFDHAIHDRVDVLSLSL
Sbjct: 241 SGASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSL 300

Query: 301 GGEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQ 333
           GGEPADYYDDGIAI+AFHAVKKGIPVVCSAGNSGP AQTVSNTAPWILTVGAST+DREFQ
Sbjct: 301 GGEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQ 360

BLAST of CsGy1G015000 vs. TrEMBL
Match: tr|A0A0A0LYF1|A0A0A0LYF1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=4 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 3.5e-141
Identity = 259/371 (69.81%), Postives = 289/371 (77.90%), Query Frame = 0

Query: 6   MKIYNSILILFSLLLIISPAMAAK------------------------------------ 65
           MK + S LI FS LL+ISPA+A K                                    
Sbjct: 1   MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60

Query: 66  ----KARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMH 125
               KA++AIFYSYKKNINGFAA +D+E+A +LA HPEVAAVLPN+AK L+TTHSWEFMH
Sbjct: 61  RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120

Query: 126 LEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCT-DKT 185
           LEKNGVIPPSS W RAK GKDVIIANLDTGVWPESKSFGEHGIVGP PSKWKGGCT DKT
Sbjct: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180

Query: 186 LDRVPCNRKLIGAKYFNKGFLAYLKSENLT---ALVINSTRDYDGHGSHTLSTAGGSYVS 245
            D VPCN+KLIGAKYFNKG+  YLKSEN T   + +INSTRDY+GHGSHTLSTAGG+YV 
Sbjct: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240

Query: 246 GASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLG 305
           GASVFG G+GTAKGGSPKARVAAYKVCWP E GGCFDADI +AFDHAIHD VDVLSLSLG
Sbjct: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300

Query: 306 GEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQA 333
            +   Y +D IAI++FHAVKKGIPVVC+ GNSGP  +T SNTAPWILTVGAST+DREF A
Sbjct: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360

BLAST of CsGy1G015000 vs. TrEMBL
Match: tr|V4TRS8|V4TRS8_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10018000mg PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 7.9e-117
Identity = 202/306 (66.01%), Postives = 250/306 (81.70%), Query Frame = 0

Query: 27  AAKKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHL 86
           + +KAR+AIFYSY+ +INGFAA ++EEEAA++AKHP+V ++ PN+ KKLHTT SW+FM L
Sbjct: 68  STEKARDAIFYSYQNHINGFAATLEEEEAAEIAKHPDVVSIFPNKGKKLHTTRSWDFMLL 127

Query: 87  EKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLD 146
           E NGVI  SSAW + + G+D+IIANLDTGVWPESKSF + G  GPVPS+WKG C + T +
Sbjct: 128 ENNGVIHSSSAWGKGRFGEDIIIANLDTGVWPESKSFSDEG-YGPVPSRWKGTCQNSTKE 187

Query: 147 RVPCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVF 206
            V CNRKLIGA+YFNK + AY+K  N++    N+ RD++GHG+HTLSTAGG+ V G +VF
Sbjct: 188 GVRCNRKLIGARYFNKAYAAYVKQHNISVNFNNTARDHEGHGTHTLSTAGGNLVPGVNVF 247

Query: 207 GLGVGTAKGGSPKARVAAYKVCWP-LEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPA 266
           G+G GTAKGGSPKARVAAYKVCWP + DG CFDADI + FD AIHD VDV+S+SLGG+PA
Sbjct: 248 GMGNGTAKGGSPKARVAAYKVCWPQVSDGQCFDADILKGFDMAIHDGVDVISVSLGGDPA 307

Query: 267 DYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVEL 326
           DY++DG AI AFHAVK GI VVCSA NSGP   TV+N +PWI+TVGAST+DREFQ  +EL
Sbjct: 308 DYFNDGTAIGAFHAVKHGIVVVCSAANSGPELGTVTNVSPWIITVGASTLDREFQNFIEL 367

Query: 327 QNGHRY 332
           +NG R+
Sbjct: 368 RNGQRF 372

BLAST of CsGy1G015000 vs. TrEMBL
Match: tr|A0A2H5N5H1|A0A2H5N5H1_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_016550 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.0e-116
Identity = 201/306 (65.69%), Postives = 250/306 (81.70%), Query Frame = 0

Query: 27  AAKKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHL 86
           + +KAR+AIFYSY+ +INGFAA ++EEEAA++AKHP+V ++ PN+ KKLHTT SW+FM L
Sbjct: 68  STEKARDAIFYSYQNHINGFAATLEEEEAAEIAKHPDVVSIFPNKGKKLHTTRSWDFMLL 127

Query: 87  EKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLD 146
           E NGVI  SSAW + + G+D++IANLDTGVWPESKSF + G  GPVPS+WKG C + T +
Sbjct: 128 ENNGVIHSSSAWGKGRFGEDIVIANLDTGVWPESKSFSDEG-YGPVPSRWKGTCQNSTKE 187

Query: 147 RVPCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVF 206
            V CNRKLIGA+YFNK + AY+K  N++    N+ RD++GHG+HTLSTAGG+ V G +VF
Sbjct: 188 GVRCNRKLIGARYFNKAYAAYVKQHNISVNFNNTARDHEGHGTHTLSTAGGNLVPGVNVF 247

Query: 207 GLGVGTAKGGSPKARVAAYKVCWP-LEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPA 266
           G+G GTAKGGSPKARVAAYKVCWP + DG CFDADI + FD AIHD VDV+S+SLGG+PA
Sbjct: 248 GMGNGTAKGGSPKARVAAYKVCWPQVSDGQCFDADILKGFDMAIHDGVDVISVSLGGDPA 307

Query: 267 DYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVEL 326
           DY++DG AI AFHAVK GI VVCSA NSGP   TV+N +PWI+TVGAST+DREFQ  +EL
Sbjct: 308 DYFNDGTAIGAFHAVKHGIVVVCSAANSGPELGTVTNVSPWIITVGASTLDREFQNFIEL 367

Query: 327 QNGHRY 332
           +NG R+
Sbjct: 368 RNGQRF 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004139597.18.7e-17699.67PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus][more]
KGN64982.18.7e-17699.67hypothetical protein Csa_1G171040 [Cucumis sativus][more]
XP_008464322.18.4e-17182.80PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo][more]
XP_023520266.14.7e-14582.03subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo][more]
XP_022927096.13.3e-14379.61subtilisin-like protease SBT5.4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G59810.16.3e-11265.26Subtilase family protein[more]
AT2G04160.16.8e-10661.18Subtilisin-like serine endopeptidase family protein[more]
AT4G10550.38.1e-7549.02Subtilase family protein[more]
AT4G10510.13.1e-7449.02Subtilase family protein[more]
AT1G32960.11.2e-7348.87Subtilase family protein[more]
Match NameE-valueIdentityDescription
sp|F4JXC5|SBT54_ARATH1.1e-11065.26Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
sp|Q9ZSP5|AIR3_ARATH1.2e-10461.18Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
sp|I1N462|SBT1_SOYBN5.0e-8250.97Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
sp|Q8L7I2|SBT36_ARATH1.5e-7349.02Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S... [more]
sp|Q9SZY2|SBT37_ARATH5.5e-7349.02Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 S... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LVY8|A0A0A0LVY8_CUCSA5.8e-17699.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=4 SV=1[more]
tr|A0A1S3CLB7|A0A1S3CLB7_CUCME5.6e-17182.80subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=4 SV=... [more]
tr|A0A0A0LYF1|A0A0A0LYF1_CUCSA3.5e-14169.81Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=4 SV=1[more]
tr|V4TRS8|V4TRS8_9ROSI7.9e-11766.01Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10018000mg PE=4 ... [more]
tr|A0A2H5N5H1|A0A2H5N5H1_CITUN1.0e-11665.69Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_016550 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR034197Peptidases_S8_3
IPR037045S8pro/Inhibitor_I9_sf
IPR036852Peptidase_S8/S53_dom_sf
IPR000209Peptidase_S8/S53_dom
IPR010259S8pro/Inhibitor_I9
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G015000.1CsGy1G015000.1mRNA


Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 29..76
e-value: 3.9E-11
score: 43.4
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 104..314
e-value: 1.3E-17
score: 64.0
IPR036852Peptidase S8/S53 domain superfamilyGENE3DG3DSA:3.40.50.200coord: 106..315
e-value: 1.2E-89
score: 303.2
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILYSSF52743Subtilisin-likecoord: 75..329
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 316..332
e-value: 1.2E-89
score: 303.2
NoneNo IPR availablePANTHERPTHR10795:SF423SUBTILISIN-LIKE PROTEASE SBT5.3coord: 28..331
NoneNo IPR availablePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 28..331
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3DG3DSA:3.30.70.80coord: 15..76
e-value: 7.3E-15
score: 57.2
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 73..315
e-value: 3.34214E-118
score: 347.278

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CsGy1G015000MELO3C026084Melon (DHL92) v3.5.1cgybmeB016
The following gene(s) are paralogous to this gene:

None