CsGy1G010880 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy1G010880
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	GA 2-oxidase
Location	Chr1 : 6852639 .. 6853151 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACCCACCATTTCACGAGGCTTACAAGACCCTCTTGGCCGAAAGGGCGGCGGCGGCGACGGGTGCAAAGGGAGCCAGCGACGTCGTCCTCACGGTGGTCGAGGAATGGGATCTTCCGCTGGTTGATTTAGAGCGTTTGACGGCGGGCAAAGTGGAAGAGGTGGAACAATGCAAAAATGACATAATTACAGCTTCTAAAGAATGGGGTTTCTTTCAAGTTGTGAATCATGGGATTTCAAATCAGCTTTTGGCCAAAATGAGAGCTAAGCAAATCGAGCTTTTCAAGCAGCCGTTTGAGCGTAAGAGTAAAGAAGATCAATTTTCGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTATTACACAACTTTCTTGGTCCGAAGCTTTTCATGTTTCTCTATCTGATATTCTTGGAACCAACGGCTCCGATGATGATGATCTCAGGTACAATATTACACCTTTTTCTTCTTCTTTTCTTTTGATGAAAATTGTTTGA

mRNA sequence

ATGGACCCACCATTTCACGAGGCTTACAAGACCCTCTTGGCCGAAAGGGCGGCGGCGGCGACGGGTGCAAAGGGAGCCAGCGACGTCGTCCTCACGGTGGTCGAGGAATGGGATCTTCCGCTGGTTGATTTAGAGCGTTTGACGGCGGGCAAAGTGGAAGAGGTGGAACAATGCAAAAATGACATAATTACAGCTTCTAAAGAATGGGGTTTCTTTCAAGTTGTGAATCATGGGATTTCAAATCAGCTTTTGGCCAAAATGAGAGCTAAGCAAATCGAGCTTTTCAAGCAGCCGTTTGAGCGTAAGAGTAAAGAAGATCAATTTTCGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTATTACACAACTTTCTTGGTCCGAAGCTTTTCATGTTTCTCTATCTGATATTCTTGGAACCAACGGCTCCGATGATGATGATCTCAGGTACAATATTACACCTTTTTCTTCTTCTTTTCTTTTGATGAAAATTGTTTGA

Coding sequence (CDS)

ATGGACCCACCATTTCACGAGGCTTACAAGACCCTCTTGGCCGAAAGGGCGGCGGCGGCGACGGGTGCAAAGGGAGCCAGCGACGTCGTCCTCACGGTGGTCGAGGAATGGGATCTTCCGCTGGTTGATTTAGAGCGTTTGACGGCGGGCAAAGTGGAAGAGGTGGAACAATGCAAAAATGACATAATTACAGCTTCTAAAGAATGGGGTTTCTTTCAAGTTGTGAATCATGGGATTTCAAATCAGCTTTTGGCCAAAATGAGAGCTAAGCAAATCGAGCTTTTCAAGCAGCCGTTTGAGCGTAAGAGTAAAGAAGATCAATTTTCGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTATTACACAACTTTCTTGGTCCGAAGCTTTTCATGTTTCTCTATCTGATATTCTTGGAACCAACGGCTCCGATGATGATGATCTCAGGTACAATATTACACCTTTTTCTTCTTCTTTTCTTTTGATGAAAATTGTTTGA

Protein sequence

MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFSSSFLLMKIV

Homology

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BLAST of CsGy1G010880 vs. NCBI nr
Match: CCL98007.1 (GA 2-oxidase, partial [Cucumis sativus] >KGN64551.1 hypothetical protein Csa_1G064730 [Cucumis sativus])

HSP 1 Score: 308.9 bits (790), Expect = 1.1e-80
Identity = 154/161 (95.65%), Postives = 157/161 (97.52%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN
Sbjct: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFS 162
           PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLR  +  ++
Sbjct: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYA 161

[Close]

BLAST of CsGy1G010880 vs. NCBI nr
Match: XP_004153456.2 (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis sativus])

HSP 1 Score: 308.9 bits (790), Expect = 1.1e-80
Identity = 154/161 (95.65%), Postives = 157/161 (97.52%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN
Sbjct: 15  MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 74

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 75  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 134

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFS 162
           PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLR  +  ++
Sbjct: 135 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYA 175

[Close]

BLAST of CsGy1G010880 vs. NCBI nr
Match: XP_008441442.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo])

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-66
Identity = 135/161 (83.85%), Postives = 141/161 (87.58%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 46  MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 105

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 106 DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 165

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFS 162
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLR  +  ++
Sbjct: 166 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYA 201

[Close]

BLAST of CsGy1G010880 vs. NCBI nr
Match: XP_008441451.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis melo])

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-66
Identity = 135/161 (83.85%), Postives = 141/161 (87.58%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 14  MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 73

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 74  DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 133

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFS 162
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLR  +  ++
Sbjct: 134 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYA 169

[Close]

BLAST of CsGy1G010880 vs. NCBI nr
Match: XP_023520137.1 (gibberellin 2-beta-dioxygenase 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 225.3 bits (573), Expect = 1.5e-55
Identity = 116/151 (76.82%), Postives = 128/151 (84.77%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAG-KVEEVEQCK 60
           MDPPFHEAYK+LLAE A    G     D V   V+EW+LP+VDL RL  G +V EVEQCK
Sbjct: 13  MDPPFHEAYKSLLAETAVDRGG-----DAV--AVKEWELPVVDLRRLRDGAEVGEVEQCK 72

Query: 61  NDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWG 120
           NDII AS+EWGFFQVVNHG+S++LL KMRAKQIELFKQPFERKSKED F NFSAGSYRWG
Sbjct: 73  NDIIRASQEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWG 132

Query: 121 TPSATSITQLSWSEAFHVSLSDILGTNGSDD 151
           TP+ATS+TQLSWSEAFHVSLSDILG+NGSDD
Sbjct: 133 TPTATSLTQLSWSEAFHVSLSDILGSNGSDD 156

[Close]

BLAST of CsGy1G010880 vs. TAIR10
Match: AT4G21200.1 (gibberellin 2-oxidase 8)

HSP 1 Score: 155.6 bits (392), Expect = 2.7e-38
Identity = 85/165 (51.52%), Postives = 109/165 (66.06%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGA---SDVVLTVVEEWDLPLVDLERLTAGKVEEVEQ 60
           MDPPF+E Y  LL  +             S  V  VVEE +LP++D+ RL  G  EE E+
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREK 60

Query: 61  CKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYR 120
           CK  I  AS+EWGFFQV+NHGIS  +L KMR +QI +F++PF++KSK ++   FSAGSYR
Sbjct: 61  CKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEK---FSAGSYR 120

Query: 121 WGTPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFSS 163
           WGTPSATSI QLSWSEAFHV ++DI  ++  D   L   +  F+S
Sbjct: 121 WGTPSATSIRQLSWSEAFHVPMTDI--SDNKDFTTLSSTMEKFAS 160

[Close]

BLAST of CsGy1G010880 vs. TAIR10
Match: AT1G50960.1 (gibberellin 2-oxidase 7)

HSP 1 Score: 105.9 bits (263), Expect = 2.5e-23
Identity = 51/104 (49.04%), Postives = 72/104 (69.23%), Query Frame = 0


Query: 39  LPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQP 98
           LP++DL  LT+G+  + ++C   ++ A+KEWGFFQ+VNHGI   +   M  ++ +LF QP
Sbjct: 40  LPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQP 99

Query: 99  FERKSKEDQFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDI 143
           F  K +E +FS+ S  SYRWG PSATS  Q S SEAFH+ LS++
Sbjct: 100 FSVKVRE-RFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEV 142

[Close]

BLAST of CsGy1G010880 vs. TAIR10
Match: AT1G17020.1 (senescence-related gene 1)

HSP 1 Score: 57.0 bits (136), Expect = 1.3e-08
Identity = 27/67 (40.30%), Postives = 42/67 (62.69%), Query Frame = 0


Query: 38  DLPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQ 97
           ++P++D++RL +    + E  K D   A KEWGFFQ+VNHGI +  L K++++  + F  
Sbjct: 52  EIPIIDMKRLCSSTTMDSEVEKLDF--ACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNL 111

Query: 98  PFERKSK 105
           P E K K
Sbjct: 112 PMEEKKK 116

[Close]

BLAST of CsGy1G010880 vs. TAIR10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 53.5 bits (127), Expect = 1.5e-07
Identity = 35/106 (33.02%), Postives = 54/106 (50.94%), Query Frame = 0


Query: 38  DLPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQ 97
           ++P++D+ RL +    + E  K D   A ++WGFFQ+VNHGI +  L K+  +  E F  
Sbjct: 52  EIPVIDMTRLCSVSAMDSELKKLDF--ACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNL 111

Query: 98  PFERKSKEDQFSNFSAGSYRWGTPSATSITQ-LSWSEAFHVSLSDI 143
           P + K K  Q S    G   +G  +  S  Q L W + F ++   I
Sbjct: 112 PMKEKQKLWQRSGEFEG---FGQVNIVSENQKLDWGDMFILTTEPI 152

[Close]

BLAST of CsGy1G010880 vs. TAIR10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 52.0 bits (123), Expect = 4.2e-07
Identity = 34/106 (32.08%), Postives = 54/106 (50.94%), Query Frame = 0


Query: 38  DLPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQ 97
           ++P++D+ RL +    + E  K D   A KE+GFFQ+VNHGI    L K++++  + F  
Sbjct: 53  EIPIIDMNRLCSSTAVDSEVEKLDF--ACKEYGFFQLVNHGIDPSFLDKIKSEIQDFFNL 112

Query: 98  PFERKSKEDQFSNFSAGSYRWGTPSATSITQ-LSWSEAFHVSLSDI 143
           P E K K  Q      G   +G     S  Q L W++ F + +  +
Sbjct: 113 PMEEKKKLWQTPAVMEG---FGQAFVVSEDQKLDWADLFFLIMQPV 153

[Close]

BLAST of CsGy1G010880 vs. Swiss-Prot
Match: sp|O49561|G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 155.6 bits (392), Expect = 4.9e-37
Identity = 85/165 (51.52%), Postives = 109/165 (66.06%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGA---SDVVLTVVEEWDLPLVDLERLTAGKVEEVEQ 60
           MDPPF+E Y  LL  +             S  V  VVEE +LP++D+ RL  G  EE E+
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREK 60

Query: 61  CKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYR 120
           CK  I  AS+EWGFFQV+NHGIS  +L KMR +QI +F++PF++KSK ++   FSAGSYR
Sbjct: 61  CKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEK---FSAGSYR 120

Query: 121 WGTPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFSS 163
           WGTPSATSI QLSWSEAFHV ++DI  ++  D   L   +  F+S
Sbjct: 121 WGTPSATSIRQLSWSEAFHVPMTDI--SDNKDFTTLSSTMEKFAS 160

[Close]

BLAST of CsGy1G010880 vs. Swiss-Prot
Match: sp|Q7XP65|G2OX6_ORYSJ (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.7e-27
Identity = 68/156 (43.59%), Postives = 90/156 (57.69%), Query Frame = 0


Query: 1   MDPPFHEAYKTL----------LAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAG 60
           +DPP  ++Y+ L          +A  A    G+ GA       V E DLP+VDLERLT G
Sbjct: 9   IDPPLADSYRALALLRRDRDGGIAPPAVQMVGSGGA-------VLERDLPMVDLERLTRG 68

Query: 61  KVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSN 120
              E + C   +  A+ EWGFFQ+ NHG+  +L+ +MR +Q  LF+ PFE K K    + 
Sbjct: 69  GAGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEK----AG 128

Query: 121 FSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGTN 147
              GSYRWG P+ATS+  LSWSEAFHV L+ I G +
Sbjct: 129 LLNGSYRWGNPTATSLRHLSWSEAFHVPLASISGAD 153

[Close]

BLAST of CsGy1G010880 vs. Swiss-Prot
Match: sp|Q9C6I4|G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 4.4e-22
Identity = 51/104 (49.04%), Postives = 72/104 (69.23%), Query Frame = 0


Query: 39  LPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQP 98
           LP++DL  LT+G+  + ++C   ++ A+KEWGFFQ+VNHGI   +   M  ++ +LF QP
Sbjct: 40  LPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQP 99

Query: 99  FERKSKEDQFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDI 143
           F  K +E +FS+ S  SYRWG PSATS  Q S SEAFH+ LS++
Sbjct: 100 FSVKVRE-RFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEV 142

[Close]

BLAST of CsGy1G010880 vs. Swiss-Prot
Match: sp|Q39224|SRG1_ARATH (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.4e-07
Identity = 27/67 (40.30%), Postives = 42/67 (62.69%), Query Frame = 0


Query: 38  DLPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQ 97
           ++P++D++RL +    + E  K D   A KEWGFFQ+VNHGI +  L K++++  + F  
Sbjct: 52  EIPIIDMKRLCSSTTMDSEVEKLDF--ACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNL 111

Query: 98  PFERKSK 105
           P E K K
Sbjct: 112 PMEEKKK 116

[Close]

BLAST of CsGy1G010880 vs. Swiss-Prot
Match: sp|D4N502|DIOX3_PAPSO (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 5.3e-07
Identity = 40/136 (29.41%), Postives = 67/136 (49.26%), Query Frame = 0


Query: 3   PPFHEAYKTLLAERAA--AATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 62
           P   E  K  LAE  +    TG    +++  +V ++  +P++DL+ L + +    +   +
Sbjct: 16  PSVQELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPEPVVGKLELD 75

Query: 63  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 122
            + +A KEWGFFQ+VNHG+   L+  ++++    F  P   K+K  Q      G   +G 
Sbjct: 76  KLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDFEG---FGQ 135

Query: 123 PSATSITQ-LSWSEAF 136
           P   S  Q L W+E F
Sbjct: 136 PYIESEDQRLDWTEVF 148

[Close]

BLAST of CsGy1G010880 vs. TrEMBL
Match: tr|S0CRC1|S0CRC1_CUCSA (GA 2-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=GA2ox5 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 7.0e-81
Identity = 154/161 (95.65%), Postives = 157/161 (97.52%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN
Sbjct: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFS 162
           PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLR  +  ++
Sbjct: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYA 161

[Close]

BLAST of CsGy1G010880 vs. TrEMBL
Match: tr|A0A1S3B3F3|A0A1S3B3F3_CUCME (gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 7.5e-67
Identity = 135/161 (83.85%), Postives = 141/161 (87.58%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 46  MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 105

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 106 DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 165

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFS 162
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLR  +  ++
Sbjct: 166 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYA 201

[Close]

BLAST of CsGy1G010880 vs. TrEMBL
Match: tr|A0A1S3B309|A0A1S3B309_CUCME (gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 7.5e-67
Identity = 135/161 (83.85%), Postives = 141/161 (87.58%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 14  MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 73

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 74  DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 133

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFS 162
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLR  +  ++
Sbjct: 134 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYA 169

[Close]

BLAST of CsGy1G010880 vs. TrEMBL
Match: tr|A0A067JAL2|A0A067JAL2_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_21273 PE=3 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.4e-41
Identity = 82/146 (56.16%), Postives = 111/146 (76.03%), Query Frame = 0


Query: 2   DPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKND 61
           DPPFHE YKTLL +    +      +D  L ++EE +LPL+DL RL+     E E+CK +
Sbjct: 4   DPPFHEIYKTLLDKTIQESKTKTATTDNELDIIEECELPLIDLSRLSLLDELEKEKCKEE 63

Query: 62  IITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTP 121
           I  AS+EWGFFQVVNHGIS ++L  MR +Q++LFKQPF++KSKED+F NFS+G+YRWGTP
Sbjct: 64  IARASQEWGFFQVVNHGISREILENMRREQVKLFKQPFDKKSKEDKFLNFSSGTYRWGTP 123

Query: 122 SATSITQLSWSEAFHVSLSDILGTNG 148
           +AT ++QLSWSEAFH+ ++DI  ++G
Sbjct: 124 TATCLSQLSWSEAFHIPMTDISASDG 149

[Close]

BLAST of CsGy1G010880 vs. TrEMBL
Match: tr|I1LFR2|I1LFR2_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=100793548 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.7e-40
Identity = 88/162 (54.32%), Postives = 117/162 (72.22%), Query Frame = 0


Query: 2   DPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKND 61
           DPPFHEAYK +L ++       +   + +L V EE DLP++DL RL        E+CK+ 
Sbjct: 6   DPPFHEAYK-ILFDKTRNGNNFEEHKE-LLAVAEECDLPVIDLSRLEESDEVVREECKSQ 65

Query: 62  IITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTP 121
           I  AS+EWGFFQVVNHGIS ++ + +R +Q ++FKQPFE+K+KED+F NFSAGSYRWGTP
Sbjct: 66  IARASQEWGFFQVVNHGISTEIFSSLRCEQEKVFKQPFEKKTKEDKFLNFSAGSYRWGTP 125

Query: 122 SATSITQLSWSEAFHVSLSDILGTNGSDDDDLRYNITPFSSS 164
           SAT I QLSWSEAFH+ L+DILG+ GS  + L + I  F+++
Sbjct: 126 SATCIKQLSWSEAFHIPLTDILGSTGS--NSLSWTIEQFATT 163

The following BLAST results are available for this feature:

BLAST of CsGy1G010880 vs. NCBI nr


Match Name	E-value	Identity	Description
CCL98007.1	1.1e-80	95.65	GA 2-oxidase, partial [Cucumis sativus] >KGN64551.1 hypothetical protein Csa_1G0...	[more]
XP_004153456.2	1.1e-80	95.65	PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis sativus]	[more]
XP_008441442.1	1.1e-66	83.85	PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo]	[more]
XP_008441451.1	1.1e-66	83.85	PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis melo]	[more]
XP_023520137.1	1.5e-55	76.82	gibberellin 2-beta-dioxygenase 8 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsGy1G010880 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G21200.1	2.7e-38	51.52	gibberellin 2-oxidase 8	[more]
AT1G50960.1	2.5e-23	49.04	gibberellin 2-oxidase 7	[more]
AT1G17020.1	1.3e-08	40.30	senescence-related gene 1	[more]
AT1G78550.1	1.5e-07	33.02	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17010.1	4.2e-07	32.08	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of CsGy1G010880 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O49561\|G2OX8_ARATH	4.9e-37	51.52	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
sp\|Q7XP65\|G2OX6_ORYSJ	2.7e-27	43.59	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
sp\|Q9C6I4\|G2OX7_ARATH	4.4e-22	49.04	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
sp\|Q39224\|SRG1_ARATH	2.4e-07	40.30	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
sp\|D4N502\|DIOX3_PAPSO	5.3e-07	29.41	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]

BLAST of CsGy1G010880 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|S0CRC1\|S0CRC1_CUCSA	7.0e-81	95.65	GA 2-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=GA2ox5 PE=2 SV=1	[more]
tr\|A0A1S3B3F3\|A0A1S3B3F3_CUCME	7.5e-67	83.85	gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]
tr\|A0A1S3B309\|A0A1S3B309_CUCME	7.5e-67	83.85	gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]
tr\|A0A067JAL2\|A0A067JAL2_JATCU	2.4e-41	56.16	Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_21273 PE=3 SV=1	[more]
tr\|I1LFR2\|I1LFR2_SOYBN	2.7e-40	54.32	Uncharacterized protein OS=Glycine max OX=3847 GN=100793548 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR027443	IPNS-like
IPR026992	DIOX_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy1G010880.1	CsGy1G010880.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 39..140 e-value: 9.5E-17 score: 61.7
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 19..163 e-value: 6.4E-18 score: 67.0
None	No IPR available	PANTHER	PTHR10209:SF386	GIBBERELLIN 2-BETA-DIOXYGENASE 8	coord: 27..161
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 27..161
None	No IPR available	SUPERFAMILY	SSF51197	Clavaminate synthase-like	coord: 32..142

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsGy1G010880	Cla019405	Watermelon (97103) v1	cgybwmB010
CsGy1G010880	Csa1G064730	Cucumber (Chinese Long) v2	cgybcuB001
CsGy1G010880	MELO3C002483	Melon (DHL92) v3.5.1	cgybmeB022
CsGy1G010880	ClCG03G004980	Watermelon (Charleston Gray)	cgybwcgB033
CsGy1G010880	CSPI01G10980	Wild cucumber (PI 183967)	cgybcpiB001
CsGy1G010880	CmaCh13G007180	Cucurbita maxima (Rimu)	cgybcmaB074
CsGy1G010880	CmoCh13G007420	Cucurbita moschata (Rifu)	cgybcmoB068
CsGy1G010880	Lsi02G006300	Bottle gourd (USVL1VR-Ls)	cgyblsiB018
CsGy1G010880	Cp4.1LG20g03710	Cucurbita pepo (Zucchini)	cgybcpeB070
CsGy1G010880	MELO3C002483.2	Melon (DHL92) v3.6.1	cgybmedB020
CsGy1G010880	CsaV3_1G011060	Cucumber (Chinese Long) v3	cgybcucB002
CsGy1G010880	Cla97C03G056160	Watermelon (97103) v2	cgybwmbB032
CsGy1G010880	Bhi08G001447	Wax gourd	cgybwgoB048
CsGy1G010880	Carg07527	Silver-seed gourd	carcgybB0992

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy1G010880	Cucumber (Chinese Long) v3	cgybcucB045
CsGy1G010880	Watermelon (97103) v2	cgybwmbB002
CsGy1G010880	Wax gourd	cgybwgoB020
CsGy1G010880	Cucumber (Gy14) v2	cgybcgybB035
CsGy1G010880	Cucurbita maxima (Rimu)	cgybcmaB093
CsGy1G010880	Cucurbita maxima (Rimu)	cgybcmaB126
CsGy1G010880	Cucurbita maxima (Rimu)	cgybcmaB142
CsGy1G010880	Cucurbita moschata (Rifu)	cgybcmoB082
CsGy1G010880	Cucurbita moschata (Rifu)	cgybcmoB116
CsGy1G010880	Cucurbita moschata (Rifu)	cgybcmoB133
CsGy1G010880	Cucurbita pepo (Zucchini)	cgybcpeB018
CsGy1G010880	Cucurbita pepo (Zucchini)	cgybcpeB023
CsGy1G010880	Cucurbita pepo (Zucchini)	cgybcpeB067
CsGy1G010880	Cucumber (Chinese Long) v2	cgybcuB039
CsGy1G010880	Bottle gourd (USVL1VR-Ls)	cgyblsiB015
CsGy1G010880	Melon (DHL92) v3.5.1	cgybmeB076
CsGy1G010880	Melon (DHL92) v3.6.1	cgybmedB072
CsGy1G010880	Watermelon (Charleston Gray)	cgybwcgB013
CsGy1G010880	Watermelon (97103) v1	cgybwmB044
CsGy1G010880	Wild cucumber (PI 183967)	cgybcpiB037
CsGy1G010880	Silver-seed gourd	carcgybB0637
CsGy1G010880	Silver-seed gourd	carcgybB0788
CsGy1G010880	Silver-seed gourd	carcgybB0919