Cp4.1LG20g03620 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG20g03620
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Description5'-adenylylsulfate reductase
LocationCp4.1LG20 : 2017792 .. 2020827 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACGTGAATGGACTTTGCACCAATCAAAACGCGCCACCTGGACAAGAAGTTCAGGATCCTCTGATCCCTAAATTCCGTCCAGGTTCAATGTATCAAATTTGATTTAAACTTCCGTTCTAAAGTTTTACAGAGCAAGAAATCACAGACGAAGAACTACATTAATTCGAAAAGCAACCGAAAGAGAGAGCAAGATTCTTTGATCCTTCATGGCGCTTGCTGCTTCTTCTGTTTCTAATTTGCTGCATTTTGCTGTTTCCTCTGATGCTAAAGGTATTGTTCACAAATGCAGTCCCACGTTTACATTATGTAGCGAAGCTGGAGATTTTTGTTAAGATCTTCTTTATGTGTTCGAATGATGCTTTGAACAGTTGCATGTTGAGTACAATGTTAATTCTTAATTGTACATATTGTTTCGATTCGCAGCTCCACGAATTGGTTCTGTTCAGCTGTTGAATTGGCCCCACGTTTTGTCTTCTGGAGTCAATTATTTACGAAGAGGATGCTGGATGAAGCCTATTAATTCGCAATCTCAACGGAATAATTTGTTTGTGCCTCTTGCGGTCAATACCCTTGCTTCTGGTAATTCTATCTAATTGTTTCTCTTTTCACCAATTAGAGTTCATTTCTGGTATTTGTGTAAAAAAAATGTTCTGGTGTGGATAATGAGGATGTTTTGGGCTTGGATCTGCAGAGGTGGTAGAGAAAGCTGAGACGGTAGACTACGATGGATTGGCTAAACACCTTGAAAATGCTTCCCCTCTGGAAATAATGGACCAAGCCCTCGAGGAATTTGGCAACGATATTGCAATTGCATTTAGGTGTGTTCTTATTCTGTCATTTGATTAAAGTGTGTCTATAGCTTTTATTATCGAACGTCACATCCCAAATGTATGAAATTTGAAATTGCTGTGCTATTCAATCTGTGGTCTGTAAGTACCACAAGATTCGTGTTTCTGGAATTTATTTATCGTGACCTAATCTTAATTGCTTCGGATTCTGCTAGTGTATTTATATCATCTCTTACGCCACCTGCGACCATTTCTTCCATTTTTCATAATCTTTTATCTACGATCAGCTGTATATGGTCGTAGAGCATAATGGAACTAATAATATTAGATTTTTGATTGTTTGATGTTCTTTCTGGATACTGAATTATAGTTTCAATGGTATTAATAATCTTCATCCTCTACATGAATTTACTAGCAAAGAATCTGGTTTCTGATTCTTGGCCAGTAAAGTTGTCAAGAATCTCCTTGTGCTCCTCTTACTAACAAAGCGTCTAACAAAAATTATCCACTGCAGTGGCGCGGAAGATGTTGCATTGATTGAATATGCTCGTTTGACTGGCCGACCATTTAGGGTGTTTAGCCTAGACACAGGGAGGTTGAATTCAGAGACATACCAATTCTTTGATGCAGTGGAGAAACATTATGGCATCCATATAGAGTATATGTTCCCAGATGCTGTTGAAGTTCAGGGTTTAGTTCGGAACAAAGGGTTGTTTTCATTCTACGAGGATGGCCATCAGGAGTGCTGTCGAGTGAGGAAGGTGAGGCCTTTGAGGAGGGCTTTAAAGGGACTCCGAGCATGGATCACCGGACAAAGGAAAGATCAATCTCCTGGAACTAGGTCTGAAATTCCTGTTGTCCAAGTTGATCCTGTTTTCGAGGGGTTGGATGGAGGCATTGGGAGCCTGATCAAGTGGAACCCAGTGGCCAACGTTGATGGTAAAGACATATGGAACTTTTTGCGGACCATGAATGTGCCTGTGAACTCTTTGCATTCTCAAGGATATGTCTCAATTGGGTGTGAGCCATGCACGAGGCCTGTCCTTCCATGGCAGCATGAAAGAGAGGGAAGATGGTGGTGGGAGGATTCCAAGGCCAAGGAATGCGGTCTTCACAAAGGAAATCTCAAACAAGGAGATATACCCCAGCTGAATGGCAATGGAAATGGAACTCCCCTGGTTAATGACATCTTCATCTCCCAAAATTTGGTGAACCTGAACAGAACAGGAATAGAAAACCTGGCCAGGCTGGGGAACCGGAAAGAACCTTGGCTTGTTGTTCTTTATGCGCCATGGTGCCAATTCTGCCAGGTAAAAATCATTTTGCACGAGAAATTTTCAGTTGATAATCGTAATGGTATCAATAAAAAGTAGAATATACATTTGTTCATCACTAATTGACATTTTTGGGCGAGTTTGTACAGGCTATGGAAGGGTCATATGTTGAATTGGGTGAGAAGTTGGCAGGGAGTGGGGTGAAAGTTGGGAAGTTCAGAGCCGACGGTGAGCAGAAGGAGTTCGCGAAAGCAGAGCTGCAGCTAGGATCGTTCCCCACAATACTCTTCTTCCCCAAACACTCATCTAAAGCAATCAAGTACCCATCAGAGAAGAGGGATGTTGACTCGTTGATGGGTTTTGTGAATGCTCTACGATGACATGTATAGATTCACATAAGCAAAAGTCAAGTCCTAAGATCCTAATACGGTCCATTAACAAAGGGAAAATACATACGGGCTCCAATCTTGTGTTGGATTCTGTACTGGCCTGAGGAAGGAAAATAACCAGTGCGATGGAAGACGAGATCAAAAATCAAAGCTTGGGCATCTTTCTGGAGCCGAGAGAGGGCATTGGGTCCTGATCTCGAAGCTCGACCATGTTCTATACCTCTGCTGTCACAAACAATGGCAGGAACATGTTCTTATGTATGTAACTCTAAACCCACCAACACAATGCCTGCTGGTTTCTGGTCACTGGCATTGAGTTTGATGCCTTGTTATTTATTATGTAACCCTGTTTTCCCAGTGATCATTATGGCCCTGCTATTGACAATTAGCAGCCTGTAACGTGAGCTTTTCGTAGTTAGTTTTGTAGCTTGAATTGTTTTTCTTCATTTGCATTGCTTCAAAATGCATGCTTTTGTATGCATACAGGCATCAGGGTATATCCAATATCATATTCCAGATCTCATGTCCTCTCTTTCTATGTTAGATTAAAAGGGAGTTTCCTCACTTTGAAATGGAAAATTGAAA

mRNA sequence

GACGTGAATGGACTTTGCACCAATCAAAACGCGCCACCTGGACAAGAAGTTCAGGATCCTCTGATCCCTAAATTCCGTCCAGGTTCAATGTATCAAATTTGATTTAAACTTCCGTTCTAAAGTTTTACAGAGCAAGAAATCACAGACGAAGAACTACATTAATTCGAAAAGCAACCGAAAGAGAGAGCAAGATTCTTTGATCCTTCATGGCGCTTGCTGCTTCTTCTGTTTCTAATTTGCTGCATTTTGCTGTTTCCTCTGATGCTAAAGCTCCACGAATTGGTTCTGTTCAGCTGTTGAATTGGCCCCACGTTTTGTCTTCTGGAGTCAATTATTTACGAAGAGGATGCTGGATGAAGCCTATTAATTCGCAATCTCAACGGAATAATTTGTTTGTGCCTCTTGCGGTCAATACCCTTGCTTCTGAGGTGGTAGAGAAAGCTGAGACGGTAGACTACGATGGATTGGCTAAACACCTTGAAAATGCTTCCCCTCTGGAAATAATGGACCAAGCCCTCGAGGAATTTGGCAACGATATTGCAATTGCATTTAGTGGCGCGGAAGATGTTGCATTGATTGAATATGCTCGTTTGACTGGCCGACCATTTAGGGTGTTTAGCCTAGACACAGGGAGGTTGAATTCAGAGACATACCAATTCTTTGATGCAGTGGAGAAACATTATGGCATCCATATAGAGTATATGTTCCCAGATGCTGTTGAAGTTCAGGGTTTAGTTCGGAACAAAGGGTTGTTTTCATTCTACGAGGATGGCCATCAGGAGTGCTGTCGAGTGAGGAAGGTGAGGCCTTTGAGGAGGGCTTTAAAGGGACTCCGAGCATGGATCACCGGACAAAGGAAAGATCAATCTCCTGGAACTAGGTCTGAAATTCCTGTTGTCCAAGTTGATCCTGTTTTCGAGGGGTTGGATGGAGGCATTGGGAGCCTGATCAAGTGGAACCCAGTGGCCAACGTTGATGGTAAAGACATATGGAACTTTTTGCGGACCATGAATGTGCCTGTGAACTCTTTGCATTCTCAAGGATATGTCTCAATTGGGTGTGAGCCATGCACGAGGCCTGTCCTTCCATGGCAGCATGAAAGAGAGGGAAGATGGTGGTGGGAGGATTCCAAGGCCAAGGAATGCGGTCTTCACAAAGGAAATCTCAAACAAGGAGATATACCCCAGCTGAATGGCAATGGAAATGGAACTCCCCTGGTTAATGACATCTTCATCTCCCAAAATTTGGTGAACCTGAACAGAACAGGAATAGAAAACCTGGCCAGGCTGGGGAACCGGAAAGAACCTTGGCTTGTTGTTCTTTATGCGCCATGGTGCCAATTCTGCCAGGCTATGGAAGGGTCATATGTTGAATTGGGTGAGAAGTTGGCAGGGAGTGGGGTGAAAGTTGGGAAGTTCAGAGCCGACGGTGAGCAGAAGGAGTTCGCGAAAGCAGAGCTGCAGCTAGGATCGTTCCCCACAATACTCTTCTTCCCCAAACACTCATCTAAAGCAATCAAGTACCCATCAGAGAAGAGGGATGTTGACTCGTTGATGGGTTTTGTGAATGCTCTACGATGACATGTATAGATTCACATAAGCAAAAGTCAAGTCCTAAGATCCTAATACGGTCCATTAACAAAGGGAAAATACATACGGGCTCCAATCTTGTGTTGGATTCTGTACTGGCCTGAGGAAGGAAAATAACCAGTGCGATGGAAGACGAGATCAAAAATCAAAGCTTGGGCATCTTTCTGGAGCCGAGAGAGGGCATTGGGTCCTGATCTCGAAGCTCGACCATGTTCTATACCTCTGCTGTCACAAACAATGGCAGGAACATGTTCTTATGTATGTAACTCTAAACCCACCAACACAATGCCTGCTGGTTTCTGGTCACTGGCATTGAGTTTGATGCCTTGTTATTTATTATGTAACCCTGTTTTCCCAGTGATCATTATGGCCCTGCTATTGACAATTAGCAGCCTGTAACGTGAGCTTTTCGTAGTTAGTTTTGTAGCTTGAATTGTTTTTCTTCATTTGCATTGCTTCAAAATGCATGCTTTTGTATGCATACAGGCATCAGGGTATATCCAATATCATATTCCAGATCTCATGTCCTCTCTTTCTATGTTAGATTAAAAGGGAGTTTCCTCACTTTGAAATGGAAAATTGAAA

Coding sequence (CDS)

ATGGCGCTTGCTGCTTCTTCTGTTTCTAATTTGCTGCATTTTGCTGTTTCCTCTGATGCTAAAGCTCCACGAATTGGTTCTGTTCAGCTGTTGAATTGGCCCCACGTTTTGTCTTCTGGAGTCAATTATTTACGAAGAGGATGCTGGATGAAGCCTATTAATTCGCAATCTCAACGGAATAATTTGTTTGTGCCTCTTGCGGTCAATACCCTTGCTTCTGAGGTGGTAGAGAAAGCTGAGACGGTAGACTACGATGGATTGGCTAAACACCTTGAAAATGCTTCCCCTCTGGAAATAATGGACCAAGCCCTCGAGGAATTTGGCAACGATATTGCAATTGCATTTAGTGGCGCGGAAGATGTTGCATTGATTGAATATGCTCGTTTGACTGGCCGACCATTTAGGGTGTTTAGCCTAGACACAGGGAGGTTGAATTCAGAGACATACCAATTCTTTGATGCAGTGGAGAAACATTATGGCATCCATATAGAGTATATGTTCCCAGATGCTGTTGAAGTTCAGGGTTTAGTTCGGAACAAAGGGTTGTTTTCATTCTACGAGGATGGCCATCAGGAGTGCTGTCGAGTGAGGAAGGTGAGGCCTTTGAGGAGGGCTTTAAAGGGACTCCGAGCATGGATCACCGGACAAAGGAAAGATCAATCTCCTGGAACTAGGTCTGAAATTCCTGTTGTCCAAGTTGATCCTGTTTTCGAGGGGTTGGATGGAGGCATTGGGAGCCTGATCAAGTGGAACCCAGTGGCCAACGTTGATGGTAAAGACATATGGAACTTTTTGCGGACCATGAATGTGCCTGTGAACTCTTTGCATTCTCAAGGATATGTCTCAATTGGGTGTGAGCCATGCACGAGGCCTGTCCTTCCATGGCAGCATGAAAGAGAGGGAAGATGGTGGTGGGAGGATTCCAAGGCCAAGGAATGCGGTCTTCACAAAGGAAATCTCAAACAAGGAGATATACCCCAGCTGAATGGCAATGGAAATGGAACTCCCCTGGTTAATGACATCTTCATCTCCCAAAATTTGGTGAACCTGAACAGAACAGGAATAGAAAACCTGGCCAGGCTGGGGAACCGGAAAGAACCTTGGCTTGTTGTTCTTTATGCGCCATGGTGCCAATTCTGCCAGGCTATGGAAGGGTCATATGTTGAATTGGGTGAGAAGTTGGCAGGGAGTGGGGTGAAAGTTGGGAAGTTCAGAGCCGACGGTGAGCAGAAGGAGTTCGCGAAAGCAGAGCTGCAGCTAGGATCGTTCCCCACAATACTCTTCTTCCCCAAACACTCATCTAAAGCAATCAAGTACCCATCAGAGAAGAGGGATGTTGACTCGTTGATGGGTTTTGTGAATGCTCTACGATGA

Protein sequence

MALAASSVSNLLHFAVSSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPINSQSQRNNLFVPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR
BLAST of Cp4.1LG20g03620 vs. Swiss-Prot
Match: APR1_ARATH (5'-adenylylsulfate reductase 1, chloroplastic OS=Arabidopsis thaliana GN=APR1 PE=1 SV=2)

HSP 1 Score: 679.9 bits (1753), Expect = 2.0e-194
Identity = 330/461 (71.58%), Postives = 387/461 (83.95%), Query Frame = 1

Query: 3   LAASSVSNLLH--FAVSSDAKAPRIGSVQLLNWPHVLSSGVNYL-RRGCWMKPINSQSQR 62
           +++SS S +++  F VS + K  +IGS++LL+  HV    +N   +R   +KP+N++ + 
Sbjct: 7   VSSSSSSGIINSRFGVSLEPKVSQIGSLRLLDRVHVAPVSLNLSGKRSSSVKPLNAEPKT 66

Query: 63  NNLFVPLAVNTLASEVVEKAETV---DYDGLAKHLENASPLEIMDQALEEFGNDIAIAFS 122
            +  +PLA  T+ +E+ E+ E V   D++ LAK LENASPLEIMD+ALE++GNDIAIAFS
Sbjct: 67  KDSMIPLAA-TMVAEIAEEVEVVEIEDFEELAKKLENASPLEIMDKALEKYGNDIAIAFS 126

Query: 123 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 182
           GAEDVALIEYA LTGRPFRVFSLDTGRLN ETY+FFDAVEKHYGI IEYMFPD+VEVQGL
Sbjct: 127 GAEDVALIEYAHLTGRPFRVFSLDTGRLNPETYRFFDAVEKHYGIRIEYMFPDSVEVQGL 186

Query: 183 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 242
           VR+KGLFSFYEDGHQECCRVRKVRPLRRALKGL+AWITGQRKDQSPGTRSEIPVVQVDPV
Sbjct: 187 VRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPVVQVDPV 246

Query: 243 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQ 302
           FEGLDGG+GSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GY+SIGCEPCT+ VLP Q
Sbjct: 247 FEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYISIGCEPCTKAVLPGQ 306

Query: 303 HEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIE 362
           HEREGRWWWED+KAKECGLHKGN+K+        NG     V DIF S+NLV L+R GIE
Sbjct: 307 HEREGRWWWEDAKAKECGLHKGNVKENS-DDAKVNGESKSAVADIFKSENLVTLSRQGIE 366

Query: 363 NLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKA 422
           NL +L NRKEPW+VVLYAPWC FCQAME SY EL +KLAGSG+KV KFRADG+QKEFAK 
Sbjct: 367 NLMKLENRKEPWIVVLYAPWCPFCQAMEASYDELADKLAGSGIKVAKFRADGDQKEFAKQ 426

Query: 423 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           ELQLGSFPTIL FPK+SS+ IKYPSEKRDV+SL  F+N +R
Sbjct: 427 ELQLGSFPTILVFPKNSSRPIKYPSEKRDVESLTSFLNLVR 465

BLAST of Cp4.1LG20g03620 vs. Swiss-Prot
Match: APR3_ARATH (5'-adenylylsulfate reductase 3, chloroplastic OS=Arabidopsis thaliana GN=APR3 PE=2 SV=2)

HSP 1 Score: 672.9 bits (1735), Expect = 2.4e-192
Identity = 327/444 (73.65%), Postives = 369/444 (83.11%), Query Frame = 1

Query: 17  SSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPINSQSQRNNLFVPLAVNTLASEVV 76
           SSD K  +IGS++LLN  +V ++ ++   +   +K +N QS      V       ASEV 
Sbjct: 22  SSDLKVTKIGSLRLLNRTNVSAASLSLSGKRSSVKALNVQSITKESIV-------ASEVT 81

Query: 77  EKAETV---DYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGAEDVALIEYARLTGRP 136
           EK + V   D++ LAK LENASPLEIMD+ALE+FGNDIAIAFSGAEDVALIEYA LTGRP
Sbjct: 82  EKLDVVEVEDFEELAKRLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEYAHLTGRP 141

Query: 137 FRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFYEDGHQEC 196
           +RVFSLDTGRLN ETY+ FD VEKHYGI IEYMFPDAVEVQ LVRNKGLFSFYEDGHQEC
Sbjct: 142 YRVFSLDTGRLNPETYRLFDTVEKHYGIRIEYMFPDAVEVQALVRNKGLFSFYEDGHQEC 201

Query: 197 CRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGSLIKWNPV 256
           CR+RKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGG+GSL+KWNPV
Sbjct: 202 CRIRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGSLVKWNPV 261

Query: 257 ANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWEDSKAKEC 316
           ANV+G D+WNFLRTM+VPVN+LH+ GYVSIGCEPCTR VLP QHEREGRWWWED+KAKEC
Sbjct: 262 ANVEGNDVWNFLRTMDVPVNTLHAAGYVSIGCEPCTRAVLPGQHEREGRWWWEDAKAKEC 321

Query: 317 GLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENLARLGNRKEPWLVVLY 376
           GLHKGN+K+        N NGT  V DIF S+N+VNL+R GIENL +L NRKE W+VVLY
Sbjct: 322 GLHKGNIKENTNGNATANVNGTASVADIFNSENVVNLSRQGIENLMKLENRKEAWIVVLY 381

Query: 377 APWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGSFPTILFFPKHS 436
           APWC FCQAME S+ EL +KL GSGVKV KFRADG+QK+FAK ELQLGSFPTIL FPK+S
Sbjct: 382 APWCPFCQAMEASFDELADKLGGSGVKVAKFRADGDQKDFAKKELQLGSFPTILVFPKNS 441

Query: 437 SKAIKYPSEKRDVDSLMGFVNALR 458
           S+ IKYPSEKRDVDSL  F+N +R
Sbjct: 442 SRPIKYPSEKRDVDSLTSFLNLVR 458

BLAST of Cp4.1LG20g03620 vs. Swiss-Prot
Match: APR2_ARATH (5'-adenylylsulfate reductase 2, chloroplastic OS=Arabidopsis thaliana GN=APR2 PE=1 SV=2)

HSP 1 Score: 659.8 bits (1701), Expect = 2.1e-188
Identity = 332/466 (71.24%), Postives = 378/466 (81.12%), Query Frame = 1

Query: 1   MALAASSVSNLL------HFAVSSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPIN 60
           MALA +S S  +          SS++KA +I S++L +  H+        +R   MKP+N
Sbjct: 1   MALAVTSSSTAISGSSFSRSGASSESKALQICSIRLSDRTHLS-------QRRYSMKPLN 60

Query: 61  SQSQ-RNNLFVPLAVNTLASEVVEKA-ETVDYDGLAKHLENASPLEIMDQALEEFGNDIA 120
           ++S  R+  +V  A   +A EV EK  E  D++ LAK LE+ASPLEIMD+ALE FG+ IA
Sbjct: 61  AESHSRSESWVTRASTLIAPEVEEKGGEVEDFEQLAKKLEDASPLEIMDKALERFGDQIA 120

Query: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180
           IAFSGAEDVALIEYARLTG+PFRVFSLDTGRLN ETY+ FDAVEK YGI IEYMFPDAVE
Sbjct: 121 IAFSGAEDVALIEYARLTGKPFRVFSLDTGRLNPETYRLFDAVEKQYGIRIEYMFPDAVE 180

Query: 181 VQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQ 240
           VQ LVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGL+AWITGQRKDQSPGTRSEIP+VQ
Sbjct: 181 VQALVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPIVQ 240

Query: 241 VDPVFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300
           VDPVFEGLDGG+GSL+KWNP+ANV+G D+WNFLRTM+VPVN+LH+QGYVSIGCEPCTRPV
Sbjct: 241 VDPVFEGLDGGVGSLVKWNPLANVEGADVWNFLRTMDVPVNALHAQGYVSIGCEPCTRPV 300

Query: 301 LPWQHEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTP-LVNDIFISQNLVNLN 360
           LP QHEREGRWWWED+KAKECGLHKGN+K+ D     G  +  P  V +IF S N+V L+
Sbjct: 301 LPGQHEREGRWWWEDAKAKECGLHKGNIKEED-----GAADSKPAAVQEIFESNNVVALS 360

Query: 361 RTGIENLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQK 420
           + G+ENL +L NRKE WLVVLYAPWC FCQAME SY+EL EKLAG GVKV KFRADGEQK
Sbjct: 361 KGGVENLLKLENRKEAWLVVLYAPWCPFCQAMEASYIELAEKLAGKGVKVAKFRADGEQK 420

Query: 421 EFAKAELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           EFAK ELQLGSFPTIL FPK + +AIKYPSE RDVDSLM FVN LR
Sbjct: 421 EFAKQELQLGSFPTILLFPKRAPRAIKYPSEHRDVDSLMSFVNLLR 454

BLAST of Cp4.1LG20g03620 vs. Swiss-Prot
Match: APR1_ORYSJ (Probable 5'-adenylylsulfate reductase 1, chloroplastic OS=Oryza sativa subsp. japonica GN=APR1 PE=2 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 9.8e-178
Identity = 318/477 (66.67%), Postives = 361/477 (75.68%), Query Frame = 1

Query: 1   MALAASSVSNLLHFAVSSDAKAPRIGSV--QLLNWPHVLSSGVNYLRRGCWM-------- 60
           MA A +S+S+  H     D KA RIG+V  Q+   P   ++     R             
Sbjct: 1   MASATASISS--HSVALRDLKAARIGAVKQQVAAAPAAGTAAARAQRARAVRPLRAAEPA 60

Query: 61  -KPINSQSQRNNLFVPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGN 120
            +P+++ +       P+A +  A+ V   A  VDY+ LA+ L+ ASPLEIMD+AL  FG+
Sbjct: 61  RQPVSASAAAAPAAAPVAEDAAAAAVDAPAPAVDYEALAQELQGASPLEIMDRALAMFGS 120

Query: 121 DIAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPD 180
           DIAIAFSGAEDVALIEYA+LTGRPFRVFSLDTGRLN ETYQ FD VEKHYGI IEYMFPD
Sbjct: 121 DIAIAFSGAEDVALIEYAKLTGRPFRVFSLDTGRLNPETYQLFDKVEKHYGIRIEYMFPD 180

Query: 181 AVEVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIP 240
           A EVQ LVR KGLFSFYEDGHQECCR RKVRPLRRAL+GLRAWITGQRKDQSPGTR+ IP
Sbjct: 181 AGEVQALVRAKGLFSFYEDGHQECCRARKVRPLRRALRGLRAWITGQRKDQSPGTRAAIP 240

Query: 241 VVQVDPVFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCT 300
           VVQVDP FEGL GG GSL+KWNPVANVDGKD+W FLR M+VPVN+LH+QGYVSIGCEPCT
Sbjct: 241 VVQVDPSFEGLAGGAGSLVKWNPVANVDGKDVWTFLRAMDVPVNALHAQGYVSIGCEPCT 300

Query: 301 RPVLPWQHEREGRWWWEDSKAKECGLHKGNL-KQGDIPQL------NGNGNGTPLVNDIF 360
           RPVLP QHEREGRWWWED+KAKECGLHKGN+  QG             NGNG+    DIF
Sbjct: 301 RPVLPGQHEREGRWWWEDAKAKECGLHKGNIDDQGGAAAAAAHKAGGANGNGSAGAPDIF 360

Query: 361 ISQNLVNLNRTGIENLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKL--AGSGVK 420
            S  +V+L R G+ENL RL +R EPWLVVLYAPWC FCQAME SY+EL E+L  AG GVK
Sbjct: 361 ESSGVVSLTRAGVENLLRLESRAEPWLVVLYAPWCPFCQAMEASYLELAERLGGAGGGVK 420

Query: 421 VGKFRADGEQKEFAKAELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           VGKFRADGEQK FA+ ELQL SFPTIL FP  +++ IKYPSEKRDVDSL+ FVN+LR
Sbjct: 421 VGKFRADGEQKAFAQQELQLQSFPTILLFPSRTARPIKYPSEKRDVDSLLAFVNSLR 475

BLAST of Cp4.1LG20g03620 vs. Swiss-Prot
Match: CYSH_PSEAE (Phosphoadenosine phosphosulfate reductase OS=Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) GN=cysH PE=1 SV=2)

HSP 1 Score: 293.9 bits (751), Expect = 3.0e-78
Identity = 130/242 (53.72%), Postives = 177/242 (73.14%), Query Frame = 1

Query: 79  AETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGAEDVALIEYARLTGRPFRVFS 138
           ++  D   LA  L + SP +I+  A E FG+++ I+FSGAEDV L++ A    R  +VFS
Sbjct: 25  SQPFDLPALASSLADKSPQDILKAAFEHFGDELWISFSGAEDVVLVDMAWKLNRNVKVFS 84

Query: 139 LDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFYEDGHQECCRVRK 198
           LDTGRL+ ETY+F D V +HYGI I+ + PD   ++ LV+ KGLFSFY DGH ECC +RK
Sbjct: 85  LDTGRLHPETYRFIDQVREHYGIAIDVLSPDPRLLEPLVKEKGLFSFYRDGHGECCGIRK 144

Query: 199 VRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGSLIKWNPVANVDG 258
           + PL+R L G+RAW TGQR+DQSPGTRS++ V+++D  F   +     L K+NP++++  
Sbjct: 145 IEPLKRKLAGVRAWATGQRRDQSPGTRSQVAVLEIDGAFSTPE---KPLYKFNPLSSMTS 204

Query: 259 KDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWEDSKAKECGLHKG 318
           +++W ++R + +P NSLH +GY+SIGCEPCTRPVLP QHEREGRWWWE++  KECGLH G
Sbjct: 205 EEVWGYIRMLELPYNSLHERGYISIGCEPCTRPVLPNQHEREGRWWWEEATHKECGLHAG 263

Query: 319 NL 321
           NL
Sbjct: 265 NL 263

BLAST of Cp4.1LG20g03620 vs. TrEMBL
Match: E5GC59_CUCME (Adenylyl-sulfate reductase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 1.9e-236
Identity = 403/455 (88.57%), Postives = 424/455 (93.19%), Query Frame = 1

Query: 6   SSVSNLLHFAVSS-DAKAPRIGSVQLLNWPHVLSSGVNYLR-RGCWM-KPINSQSQRNNL 65
           SS SN  HFA SS + K PRIGSVQLL+ PHVLSSGVNY +  GC + KP+N+QS+R++ 
Sbjct: 11  SSSSNFHHFAASSSEPKVPRIGSVQLLHRPHVLSSGVNYSQITGCSVVKPMNAQSKRDDS 70

Query: 66  FVPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGAEDVA 125
            VP   NTLA+EV EK E VDYDGLA+ LENASPLEIMDQALE+FG+DIAIAFSGAEDVA
Sbjct: 71  LVPFVANTLAAEVAEKTEIVDYDGLARDLENASPLEIMDQALEKFGDDIAIAFSGAEDVA 130

Query: 126 LIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGL 185
           LIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGL
Sbjct: 131 LIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGL 190

Query: 186 FSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDG 245
           FSFY+DGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRS++PVVQVDPVFEGLDG
Sbjct: 191 FSFYDDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSDVPVVQVDPVFEGLDG 250

Query: 246 GIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGR 305
           GIGSLIKWNPVANV GKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP QHEREGR
Sbjct: 251 GIGSLIKWNPVANVHGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGR 310

Query: 306 WWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENLARLG 365
           WWWEDSKAKECGLHKGNLKQGD+PQLNGNGN  P VNDIFISQ+LVNLNRTG+ENLARL 
Sbjct: 311 WWWEDSKAKECGLHKGNLKQGDVPQLNGNGNEIPPVNDIFISQDLVNLNRTGMENLARLD 370

Query: 366 NRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGS 425
           NRKEPWLVVLYAPWCQFCQAMEGSYVEL EKLAGSGVKVGKFRADGEQK+FAK ELQLGS
Sbjct: 371 NRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRADGEQKQFAKQELQLGS 430

Query: 426 FPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           FPTILFFPKHSSKAIKYPSEKRDV+SLM FVNALR
Sbjct: 431 FPTILFFPKHSSKAIKYPSEKRDVESLMAFVNALR 465

BLAST of Cp4.1LG20g03620 vs. TrEMBL
Match: A0A0A0LUS5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G062380 PE=4 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 2.1e-235
Identity = 399/459 (86.93%), Postives = 423/459 (92.16%), Query Frame = 1

Query: 1   MALAASSVSNLLHFAVSSDAKAPRIGSVQLLNWPHVLSSGVNYLR-RGCWM-KPINSQSQ 60
           ++L++SS S+  HFA SSD   P IGS+QLL+ PHVLSSGVNY R RGC + KP+N+QS+
Sbjct: 3   LSLSSSSSSHFHHFAASSDPNVPPIGSLQLLHPPHVLSSGVNYSRIRGCSVVKPMNAQSK 62

Query: 61  RNNLFVPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGA 120
           RN+  VP   NTLA EV E+ E VDYDGLAK LENASPLEIMDQAL +FG+DIAIAFSGA
Sbjct: 63  RNDSLVPFVANTLAPEVAERTEIVDYDGLAKDLENASPLEIMDQALGKFGDDIAIAFSGA 122

Query: 121 EDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVR 180
           EDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEK YGIHIEYMFPDAVEVQGLVR
Sbjct: 123 EDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKQYGIHIEYMFPDAVEVQGLVR 182

Query: 181 NKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFE 240
           NKGLFSFY+DGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSE+PVVQVDPVFE
Sbjct: 183 NKGLFSFYDDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEVPVVQVDPVFE 242

Query: 241 GLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHE 300
           GLDGGIGSLIKWNPVANV GKD+WNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP QHE
Sbjct: 243 GLDGGIGSLIKWNPVANVHGKDVWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHE 302

Query: 301 REGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENL 360
           REGRWWWEDSKAKECGLHKGNLKQGD+PQLNGNGN TP VNDIFISQNLVNLNRTG+ENL
Sbjct: 303 REGRWWWEDSKAKECGLHKGNLKQGDVPQLNGNGNETPSVNDIFISQNLVNLNRTGMENL 362

Query: 361 ARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAEL 420
            RL NRKEPWLVVLYAPWCQFCQAMEGSYVEL +KLAGSGVKVGKFRADGEQK+FAK EL
Sbjct: 363 TRLDNRKEPWLVVLYAPWCQFCQAMEGSYVELADKLAGSGVKVGKFRADGEQKQFAKQEL 422

Query: 421 QLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           +LGSFPTIL FPKHSSKAIKYPSEKRDV+SL  FVNALR
Sbjct: 423 ELGSFPTILLFPKHSSKAIKYPSEKRDVESLTAFVNALR 461

BLAST of Cp4.1LG20g03620 vs. TrEMBL
Match: A0A0A0KHT4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G513670 PE=4 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 1.7e-213
Identity = 364/463 (78.62%), Postives = 407/463 (87.90%), Query Frame = 1

Query: 1   MALAASSVSNLL---HFAVS---SDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPIN 60
           MALA+SS ++ +    F+ S   S+ KAP+IGSV+L + P+V +S VNY +R C ++P++
Sbjct: 1   MALASSSSASAITGSSFSRSISASEPKAPQIGSVRLFSRPNVPASSVNYSQRRCSIEPLH 60

Query: 61  SQSQRNNLFVPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIA 120
           ++ +RN+   PLA  + A EVVE+ E  DY+GLAK LE+ASPLEIMD+ALE+FGNDIAIA
Sbjct: 61  AEPKRNDSVAPLAAASTAPEVVEEVEVEDYEGLAKDLESASPLEIMDKALEKFGNDIAIA 120

Query: 121 FSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQ 180
           FSGAEDVALIEYA LTGRP+RVFSLDTGRLN ETY+FFD VEKHYGIHIEYMFPDAVEVQ
Sbjct: 121 FSGAEDVALIEYAHLTGRPYRVFSLDTGRLNPETYKFFDEVEKHYGIHIEYMFPDAVEVQ 180

Query: 181 GLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVD 240
            LVR+KGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSE+PVVQVD
Sbjct: 181 ALVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEVPVVQVD 240

Query: 241 PVFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP 300
           PVFEGLDGGIGSL+KWNPVANV  KDIW+FLR+MNVPVN+LHSQGYVSIGCEPCTRPVLP
Sbjct: 241 PVFEGLDGGIGSLVKWNPVANVGSKDIWDFLRSMNVPVNTLHSQGYVSIGCEPCTRPVLP 300

Query: 301 WQHEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTG 360
           WQHEREGRWWWED+ AKECGLHKGNLKQ D  QLNG+ NG     DIF SQNLV+L R G
Sbjct: 301 WQHEREGRWWWEDANAKECGLHKGNLKQEDPAQLNGDANGISTDADIFESQNLVSLTRGG 360

Query: 361 IENLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFA 420
           IENLARL  RKEPW+VVLYAPWC+FCQAMEGSYVEL EKLAG+GVKVGKFRADGE+KEFA
Sbjct: 361 IENLARLEGRKEPWIVVLYAPWCRFCQAMEGSYVELAEKLAGTGVKVGKFRADGEEKEFA 420

Query: 421 KAELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           + ELQLGSFPTILFFPKHSS+ IKYPSEKRDVDSLM FVNA R
Sbjct: 421 QQELQLGSFPTILFFPKHSSRPIKYPSEKRDVDSLMAFVNAFR 463

BLAST of Cp4.1LG20g03620 vs. TrEMBL
Match: W9QV50_9ROSA (5'-adenylylsulfate reductase 1 OS=Morus notabilis GN=L484_018046 PE=4 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 2.8e-211
Identity = 359/445 (80.67%), Postives = 392/445 (88.09%), Query Frame = 1

Query: 15  AVSSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPINSQSQRNNLFVPLAVNTLASE 74
           + SS+ KA +IGSV+LL   H  SS +N  +R   +KP+N++ +RN++ VP A   +A E
Sbjct: 22  SASSELKASQIGSVRLLERSHGQSSALNISQRKSAVKPLNAEPKRNDMIVPFAATAIAPE 81

Query: 75  VVEK--AETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGAEDVALIEYARLTGR 134
           V EK   E  DY+ LAK LENASPLEIMD+ALE+FGNDIAIAFSGAEDV LIEYA LTGR
Sbjct: 82  VEEKEKVEAEDYEQLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVVLIEYAYLTGR 141

Query: 135 PFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFYEDGHQE 194
           PFRVFSLDTGRLN ETY+FFDAVEKHYGI IEYMFPDAVEVQ LVR+KGLFSFYEDGHQE
Sbjct: 142 PFRVFSLDTGRLNPETYRFFDAVEKHYGIRIEYMFPDAVEVQALVRSKGLFSFYEDGHQE 201

Query: 195 CCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGSLIKWNP 254
           CCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGSL+KWNP
Sbjct: 202 CCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGSLVKWNP 261

Query: 255 VANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWEDSKAKE 314
           VANV+G DIWNFLRTM+VPVNSLHSQGYVSIGCEPCTRPVLP QHEREGRWWWED+KAKE
Sbjct: 262 VANVNGVDIWNFLRTMDVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWEDAKAKE 321

Query: 315 CGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENLARLGNRKEPWLVVL 374
           CGLHKGN+KQ D  QLN NGNG     DIF SQNLV L+R GIENLA+LG+RKEPW+VVL
Sbjct: 322 CGLHKGNIKQEDTAQLNSNGNGAAAETDIFNSQNLVTLSRAGIENLAKLGSRKEPWIVVL 381

Query: 375 YAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGSFPTILFFPKH 434
           YAPWCQFCQAMEGSY+EL +KLAGSGVKVGKFRADGEQKE+AKAELQL SFPTILFFP H
Sbjct: 382 YAPWCQFCQAMEGSYLELADKLAGSGVKVGKFRADGEQKEYAKAELQLQSFPTILFFPGH 441

Query: 435 SSKAIKYPSEKRDVDSLMGFVNALR 458
           S++ IKYPSEKRDVDSLM FVNALR
Sbjct: 442 SAQPIKYPSEKRDVDSLMAFVNALR 466

BLAST of Cp4.1LG20g03620 vs. TrEMBL
Match: A0A067JAD3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21207 PE=4 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 8.9e-210
Identity = 356/454 (78.41%), Postives = 392/454 (86.34%), Query Frame = 1

Query: 4   AASSVSNLLHFAVSSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPINSQSQRNNLF 63
           AA S +N       S+ K  +IGS +LL+      S VN  +R C  KP+N++S+RN+  
Sbjct: 13  AAISATNFSRSTPPSELKVSQIGSFRLLD----RLSAVNISQRRCAAKPVNAESKRNDSI 72

Query: 64  VPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGAEDVAL 123
           VPLA      EV EK E  DY+ LAK LE +SPLEIMD+ALE+FGNDIAIAFSGAEDVAL
Sbjct: 73  VPLAATVAEPEVAEKVEVEDYELLAKDLETSSPLEIMDKALEKFGNDIAIAFSGAEDVAL 132

Query: 124 IEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLF 183
           IEYA+LTGRPFRVFSLDTGRLN+ETY+FFD VEK YGIHIEYMFPDAVEVQGLVR+KGLF
Sbjct: 133 IEYAKLTGRPFRVFSLDTGRLNAETYRFFDTVEKRYGIHIEYMFPDAVEVQGLVRSKGLF 192

Query: 184 SFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGG 243
           SFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSE+PVVQVDPVFEG+DGG
Sbjct: 193 SFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEVPVVQVDPVFEGMDGG 252

Query: 244 IGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGRW 303
           +GSLIKWNPVANVDG+DIWNFLRTM VPVNSLHSQGYVSIGCEPCTRPVLP QHEREGRW
Sbjct: 253 VGSLIKWNPVANVDGRDIWNFLRTMEVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRW 312

Query: 304 WWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENLARLGN 363
           WWED+KAKECGLHKGNLKQ D  QLNGNGN     +DIF SQNLV+LNRTGIENL +L N
Sbjct: 313 WWEDAKAKECGLHKGNLKQDDADQLNGNGNKATTFSDIFNSQNLVDLNRTGIENLLKLEN 372

Query: 364 RKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGSF 423
           RKEPW+VVLYAPWC+FCQ ME SY+EL +KLAGSGVKVGKFRADG+QKEFA+ +LQLGSF
Sbjct: 373 RKEPWIVVLYAPWCKFCQGMEQSYLELADKLAGSGVKVGKFRADGDQKEFARKKLQLGSF 432

Query: 424 PTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           PTIL FPKHSS+ IKYPSEKRDVDSL+ FVNALR
Sbjct: 433 PTILLFPKHSSRPIKYPSEKRDVDSLLTFVNALR 462

BLAST of Cp4.1LG20g03620 vs. TAIR10
Match: AT4G04610.1 (AT4G04610.1 APS reductase 1)

HSP 1 Score: 679.9 bits (1753), Expect = 1.1e-195
Identity = 330/461 (71.58%), Postives = 387/461 (83.95%), Query Frame = 1

Query: 3   LAASSVSNLLH--FAVSSDAKAPRIGSVQLLNWPHVLSSGVNYL-RRGCWMKPINSQSQR 62
           +++SS S +++  F VS + K  +IGS++LL+  HV    +N   +R   +KP+N++ + 
Sbjct: 7   VSSSSSSGIINSRFGVSLEPKVSQIGSLRLLDRVHVAPVSLNLSGKRSSSVKPLNAEPKT 66

Query: 63  NNLFVPLAVNTLASEVVEKAETV---DYDGLAKHLENASPLEIMDQALEEFGNDIAIAFS 122
            +  +PLA  T+ +E+ E+ E V   D++ LAK LENASPLEIMD+ALE++GNDIAIAFS
Sbjct: 67  KDSMIPLAA-TMVAEIAEEVEVVEIEDFEELAKKLENASPLEIMDKALEKYGNDIAIAFS 126

Query: 123 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 182
           GAEDVALIEYA LTGRPFRVFSLDTGRLN ETY+FFDAVEKHYGI IEYMFPD+VEVQGL
Sbjct: 127 GAEDVALIEYAHLTGRPFRVFSLDTGRLNPETYRFFDAVEKHYGIRIEYMFPDSVEVQGL 186

Query: 183 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 242
           VR+KGLFSFYEDGHQECCRVRKVRPLRRALKGL+AWITGQRKDQSPGTRSEIPVVQVDPV
Sbjct: 187 VRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPVVQVDPV 246

Query: 243 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQ 302
           FEGLDGG+GSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GY+SIGCEPCT+ VLP Q
Sbjct: 247 FEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYISIGCEPCTKAVLPGQ 306

Query: 303 HEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIE 362
           HEREGRWWWED+KAKECGLHKGN+K+        NG     V DIF S+NLV L+R GIE
Sbjct: 307 HEREGRWWWEDAKAKECGLHKGNVKENS-DDAKVNGESKSAVADIFKSENLVTLSRQGIE 366

Query: 363 NLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKA 422
           NL +L NRKEPW+VVLYAPWC FCQAME SY EL +KLAGSG+KV KFRADG+QKEFAK 
Sbjct: 367 NLMKLENRKEPWIVVLYAPWCPFCQAMEASYDELADKLAGSGIKVAKFRADGDQKEFAKQ 426

Query: 423 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           ELQLGSFPTIL FPK+SS+ IKYPSEKRDV+SL  F+N +R
Sbjct: 427 ELQLGSFPTILVFPKNSSRPIKYPSEKRDVESLTSFLNLVR 465

BLAST of Cp4.1LG20g03620 vs. TAIR10
Match: AT4G21990.1 (AT4G21990.1 APS reductase 3)

HSP 1 Score: 672.9 bits (1735), Expect = 1.4e-193
Identity = 327/444 (73.65%), Postives = 369/444 (83.11%), Query Frame = 1

Query: 17  SSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPINSQSQRNNLFVPLAVNTLASEVV 76
           SSD K  +IGS++LLN  +V ++ ++   +   +K +N QS      V       ASEV 
Sbjct: 22  SSDLKVTKIGSLRLLNRTNVSAASLSLSGKRSSVKALNVQSITKESIV-------ASEVT 81

Query: 77  EKAETV---DYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGAEDVALIEYARLTGRP 136
           EK + V   D++ LAK LENASPLEIMD+ALE+FGNDIAIAFSGAEDVALIEYA LTGRP
Sbjct: 82  EKLDVVEVEDFEELAKRLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEYAHLTGRP 141

Query: 137 FRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFYEDGHQEC 196
           +RVFSLDTGRLN ETY+ FD VEKHYGI IEYMFPDAVEVQ LVRNKGLFSFYEDGHQEC
Sbjct: 142 YRVFSLDTGRLNPETYRLFDTVEKHYGIRIEYMFPDAVEVQALVRNKGLFSFYEDGHQEC 201

Query: 197 CRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGSLIKWNPV 256
           CR+RKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGG+GSL+KWNPV
Sbjct: 202 CRIRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGVGSLVKWNPV 261

Query: 257 ANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWEDSKAKEC 316
           ANV+G D+WNFLRTM+VPVN+LH+ GYVSIGCEPCTR VLP QHEREGRWWWED+KAKEC
Sbjct: 262 ANVEGNDVWNFLRTMDVPVNTLHAAGYVSIGCEPCTRAVLPGQHEREGRWWWEDAKAKEC 321

Query: 317 GLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENLARLGNRKEPWLVVLY 376
           GLHKGN+K+        N NGT  V DIF S+N+VNL+R GIENL +L NRKE W+VVLY
Sbjct: 322 GLHKGNIKENTNGNATANVNGTASVADIFNSENVVNLSRQGIENLMKLENRKEAWIVVLY 381

Query: 377 APWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGSFPTILFFPKHS 436
           APWC FCQAME S+ EL +KL GSGVKV KFRADG+QK+FAK ELQLGSFPTIL FPK+S
Sbjct: 382 APWCPFCQAMEASFDELADKLGGSGVKVAKFRADGDQKDFAKKELQLGSFPTILVFPKNS 441

Query: 437 SKAIKYPSEKRDVDSLMGFVNALR 458
           S+ IKYPSEKRDVDSL  F+N +R
Sbjct: 442 SRPIKYPSEKRDVDSLTSFLNLVR 458

BLAST of Cp4.1LG20g03620 vs. TAIR10
Match: AT1G62180.1 (AT1G62180.1 5'adenylylphosphosulfate reductase 2)

HSP 1 Score: 659.8 bits (1701), Expect = 1.2e-189
Identity = 332/466 (71.24%), Postives = 378/466 (81.12%), Query Frame = 1

Query: 1   MALAASSVSNLL------HFAVSSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPIN 60
           MALA +S S  +          SS++KA +I S++L +  H+        +R   MKP+N
Sbjct: 1   MALAVTSSSTAISGSSFSRSGASSESKALQICSIRLSDRTHLS-------QRRYSMKPLN 60

Query: 61  SQSQ-RNNLFVPLAVNTLASEVVEKA-ETVDYDGLAKHLENASPLEIMDQALEEFGNDIA 120
           ++S  R+  +V  A   +A EV EK  E  D++ LAK LE+ASPLEIMD+ALE FG+ IA
Sbjct: 61  AESHSRSESWVTRASTLIAPEVEEKGGEVEDFEQLAKKLEDASPLEIMDKALERFGDQIA 120

Query: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180
           IAFSGAEDVALIEYARLTG+PFRVFSLDTGRLN ETY+ FDAVEK YGI IEYMFPDAVE
Sbjct: 121 IAFSGAEDVALIEYARLTGKPFRVFSLDTGRLNPETYRLFDAVEKQYGIRIEYMFPDAVE 180

Query: 181 VQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQ 240
           VQ LVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGL+AWITGQRKDQSPGTRSEIP+VQ
Sbjct: 181 VQALVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPIVQ 240

Query: 241 VDPVFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300
           VDPVFEGLDGG+GSL+KWNP+ANV+G D+WNFLRTM+VPVN+LH+QGYVSIGCEPCTRPV
Sbjct: 241 VDPVFEGLDGGVGSLVKWNPLANVEGADVWNFLRTMDVPVNALHAQGYVSIGCEPCTRPV 300

Query: 301 LPWQHEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTP-LVNDIFISQNLVNLN 360
           LP QHEREGRWWWED+KAKECGLHKGN+K+ D     G  +  P  V +IF S N+V L+
Sbjct: 301 LPGQHEREGRWWWEDAKAKECGLHKGNIKEED-----GAADSKPAAVQEIFESNNVVALS 360

Query: 361 RTGIENLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQK 420
           + G+ENL +L NRKE WLVVLYAPWC FCQAME SY+EL EKLAG GVKV KFRADGEQK
Sbjct: 361 KGGVENLLKLENRKEAWLVVLYAPWCPFCQAMEASYIELAEKLAGKGVKVAKFRADGEQK 420

Query: 421 EFAKAELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           EFAK ELQLGSFPTIL FPK + +AIKYPSE RDVDSLM FVN LR
Sbjct: 421 EFAKQELQLGSFPTILLFPKRAPRAIKYPSEHRDVDSLMSFVNLLR 454

BLAST of Cp4.1LG20g03620 vs. TAIR10
Match: AT5G60640.1 (AT5G60640.1 PDI-like 1-4)

HSP 1 Score: 50.4 bits (119), Expect = 3.3e-06
Identity = 34/109 (31.19%), Postives = 52/109 (47.71%), Query Frame = 1

Query: 321 KQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENLARLGNRKEPWLVVLYAPWCQFC 380
           ++GD   L GN +  PL       +++V +      N   +    +  LV  YAPWC  C
Sbjct: 80  EEGDFSDL-GNPDSDPLPTPEIDEKDVVVIKE---RNFTDVIENNQYVLVEFYAPWCGHC 139

Query: 381 QAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGSFPTILFF 430
           Q++   Y     +L   GV + K  A  E+ E A+ E ++  FPT+LFF
Sbjct: 140 QSLAPEYAAAATELKEDGVVLAKIDAT-EENELAQ-EYRVQGFPTLLFF 182

BLAST of Cp4.1LG20g03620 vs. TAIR10
Match: AT3G54960.1 (AT3G54960.1 PDI-like 1-3)

HSP 1 Score: 49.3 bits (116), Expect = 7.4e-06
Identity = 28/63 (44.44%), Postives = 36/63 (57.14%), Query Frame = 1

Query: 369 LVVLYAPWCQFCQAMEGSYVELGEKLAG-SGVKVGKFRADGEQKEFAKAELQLGSFPTIL 428
           L+ +YAPWC  CQ+ E  Y +LG+ L G   + V K   DG   E  +A+     FPTIL
Sbjct: 459 LLEIYAPWCGHCQSFEPIYNKLGKYLKGIDSLVVAKM--DGTSNEHPRAKAD--GFPTIL 517

Query: 429 FFP 431
           FFP
Sbjct: 519 FFP 517

BLAST of Cp4.1LG20g03620 vs. NCBI nr
Match: gi|659067724|ref|XP_008440977.1| (PREDICTED: 5'-adenylylsulfate reductase 1, chloroplastic [Cucumis melo])

HSP 1 Score: 826.2 bits (2133), Expect = 2.7e-236
Identity = 403/455 (88.57%), Postives = 424/455 (93.19%), Query Frame = 1

Query: 6   SSVSNLLHFAVSS-DAKAPRIGSVQLLNWPHVLSSGVNYLR-RGCWM-KPINSQSQRNNL 65
           SS SN  HFA SS + K PRIGSVQLL+ PHVLSSGVNY +  GC + KP+N+QS+R++ 
Sbjct: 11  SSSSNFHHFAASSSEPKVPRIGSVQLLHRPHVLSSGVNYSQITGCSVVKPMNAQSKRDDS 70

Query: 66  FVPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGAEDVA 125
            VP   NTLA+EV EK E VDYDGLA+ LENASPLEIMDQALE+FG+DIAIAFSGAEDVA
Sbjct: 71  LVPFVANTLAAEVAEKTEIVDYDGLARDLENASPLEIMDQALEKFGDDIAIAFSGAEDVA 130

Query: 126 LIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGL 185
           LIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGL
Sbjct: 131 LIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGL 190

Query: 186 FSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDG 245
           FSFY+DGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRS++PVVQVDPVFEGLDG
Sbjct: 191 FSFYDDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSDVPVVQVDPVFEGLDG 250

Query: 246 GIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGR 305
           GIGSLIKWNPVANV GKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP QHEREGR
Sbjct: 251 GIGSLIKWNPVANVHGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGR 310

Query: 306 WWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENLARLG 365
           WWWEDSKAKECGLHKGNLKQGD+PQLNGNGN  P VNDIFISQ+LVNLNRTG+ENLARL 
Sbjct: 311 WWWEDSKAKECGLHKGNLKQGDVPQLNGNGNEIPPVNDIFISQDLVNLNRTGMENLARLD 370

Query: 366 NRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGS 425
           NRKEPWLVVLYAPWCQFCQAMEGSYVEL EKLAGSGVKVGKFRADGEQK+FAK ELQLGS
Sbjct: 371 NRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRADGEQKQFAKQELQLGS 430

Query: 426 FPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           FPTILFFPKHSSKAIKYPSEKRDV+SLM FVNALR
Sbjct: 431 FPTILFFPKHSSKAIKYPSEKRDVESLMAFVNALR 465

BLAST of Cp4.1LG20g03620 vs. NCBI nr
Match: gi|778658388|ref|XP_011652629.1| (PREDICTED: 5'-adenylylsulfate reductase 1, chloroplastic [Cucumis sativus])

HSP 1 Score: 822.8 bits (2124), Expect = 3.0e-235
Identity = 399/459 (86.93%), Postives = 423/459 (92.16%), Query Frame = 1

Query: 1   MALAASSVSNLLHFAVSSDAKAPRIGSVQLLNWPHVLSSGVNYLR-RGCWM-KPINSQSQ 60
           ++L++SS S+  HFA SSD   P IGS+QLL+ PHVLSSGVNY R RGC + KP+N+QS+
Sbjct: 3   LSLSSSSSSHFHHFAASSDPNVPPIGSLQLLHPPHVLSSGVNYSRIRGCSVVKPMNAQSK 62

Query: 61  RNNLFVPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGA 120
           RN+  VP   NTLA EV E+ E VDYDGLAK LENASPLEIMDQAL +FG+DIAIAFSGA
Sbjct: 63  RNDSLVPFVANTLAPEVAERTEIVDYDGLAKDLENASPLEIMDQALGKFGDDIAIAFSGA 122

Query: 121 EDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVR 180
           EDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEK YGIHIEYMFPDAVEVQGLVR
Sbjct: 123 EDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKQYGIHIEYMFPDAVEVQGLVR 182

Query: 181 NKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFE 240
           NKGLFSFY+DGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSE+PVVQVDPVFE
Sbjct: 183 NKGLFSFYDDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEVPVVQVDPVFE 242

Query: 241 GLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHE 300
           GLDGGIGSLIKWNPVANV GKD+WNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP QHE
Sbjct: 243 GLDGGIGSLIKWNPVANVHGKDVWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHE 302

Query: 301 REGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENL 360
           REGRWWWEDSKAKECGLHKGNLKQGD+PQLNGNGN TP VNDIFISQNLVNLNRTG+ENL
Sbjct: 303 REGRWWWEDSKAKECGLHKGNLKQGDVPQLNGNGNETPSVNDIFISQNLVNLNRTGMENL 362

Query: 361 ARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAEL 420
            RL NRKEPWLVVLYAPWCQFCQAMEGSYVEL +KLAGSGVKVGKFRADGEQK+FAK EL
Sbjct: 363 TRLDNRKEPWLVVLYAPWCQFCQAMEGSYVELADKLAGSGVKVGKFRADGEQKQFAKQEL 422

Query: 421 QLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           +LGSFPTIL FPKHSSKAIKYPSEKRDV+SL  FVNALR
Sbjct: 423 ELGSFPTILLFPKHSSKAIKYPSEKRDVESLTAFVNALR 461

BLAST of Cp4.1LG20g03620 vs. NCBI nr
Match: gi|659078978|ref|XP_008440009.1| (PREDICTED: 5'-adenylylsulfate reductase 1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 752.7 bits (1942), Expect = 3.8e-214
Identity = 365/464 (78.66%), Postives = 404/464 (87.07%), Query Frame = 1

Query: 1   MALAASSVS-------NLLHFAVSSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPI 60
           MALA+SS S       +      +S+ KAP+IGSV+LL+ P+V +S VNY +R C ++P+
Sbjct: 1   MALASSSSSASAITGSSFSRSISASEPKAPQIGSVRLLSRPNVPASSVNYSQRRCSIEPL 60

Query: 61  NSQSQRNNLFVPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAI 120
           +++ +RN+   PLA  +   EVVE+ E  DY+GLAK LENASPLEIMD+ALE+FGNDIAI
Sbjct: 61  HAEPKRNDSVAPLAAASAPPEVVEEVEVEDYEGLAKGLENASPLEIMDKALEKFGNDIAI 120

Query: 121 AFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEV 180
           AFSGAEDVALIEYA LTGRPFRVFSLDTGRLN ETY+FFD VEKHYGIHIEYMFPDAVEV
Sbjct: 121 AFSGAEDVALIEYAHLTGRPFRVFSLDTGRLNPETYKFFDEVEKHYGIHIEYMFPDAVEV 180

Query: 181 QGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV 240
           Q LVR+KGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSE+PVVQV
Sbjct: 181 QALVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEVPVVQV 240

Query: 241 DPVFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVL 300
           DPVFEGLDGGIGSL+KWNP ANV  KDIW+FLR+MNVPVN+LHSQGYVSIGCEPCTRPVL
Sbjct: 241 DPVFEGLDGGIGSLVKWNPFANVGSKDIWDFLRSMNVPVNTLHSQGYVSIGCEPCTRPVL 300

Query: 301 PWQHEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRT 360
           PWQHEREGRWWWED+KAKECGLHKGNLKQ D  QLNGN NG     DIF SQNLV+L R 
Sbjct: 301 PWQHEREGRWWWEDAKAKECGLHKGNLKQEDPAQLNGNANGISTDADIFESQNLVSLTRG 360

Query: 361 GIENLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEF 420
           GIENLARL  RKEPW+VVLYAPWC+FCQAMEGSYVEL EKLAG+GVKVGKFRADG++KEF
Sbjct: 361 GIENLARLEGRKEPWIVVLYAPWCRFCQAMEGSYVELAEKLAGTGVKVGKFRADGDEKEF 420

Query: 421 AKAELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           A+ ELQLGSFPTILFFPKHSS+ IKYPSEKRDVDSLM FVNA R
Sbjct: 421 AQQELQLGSFPTILFFPKHSSRPIKYPSEKRDVDSLMAFVNAFR 464

BLAST of Cp4.1LG20g03620 vs. NCBI nr
Match: gi|449433968|ref|XP_004134768.1| (PREDICTED: 5'-adenylylsulfate reductase 1, chloroplastic [Cucumis sativus])

HSP 1 Score: 750.0 bits (1935), Expect = 2.5e-213
Identity = 364/463 (78.62%), Postives = 407/463 (87.90%), Query Frame = 1

Query: 1   MALAASSVSNLL---HFAVS---SDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPIN 60
           MALA+SS ++ +    F+ S   S+ KAP+IGSV+L + P+V +S VNY +R C ++P++
Sbjct: 1   MALASSSSASAITGSSFSRSISASEPKAPQIGSVRLFSRPNVPASSVNYSQRRCSIEPLH 60

Query: 61  SQSQRNNLFVPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIA 120
           ++ +RN+   PLA  + A EVVE+ E  DY+GLAK LE+ASPLEIMD+ALE+FGNDIAIA
Sbjct: 61  AEPKRNDSVAPLAAASTAPEVVEEVEVEDYEGLAKDLESASPLEIMDKALEKFGNDIAIA 120

Query: 121 FSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQ 180
           FSGAEDVALIEYA LTGRP+RVFSLDTGRLN ETY+FFD VEKHYGIHIEYMFPDAVEVQ
Sbjct: 121 FSGAEDVALIEYAHLTGRPYRVFSLDTGRLNPETYKFFDEVEKHYGIHIEYMFPDAVEVQ 180

Query: 181 GLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVD 240
            LVR+KGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSE+PVVQVD
Sbjct: 181 ALVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEVPVVQVD 240

Query: 241 PVFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP 300
           PVFEGLDGGIGSL+KWNPVANV  KDIW+FLR+MNVPVN+LHSQGYVSIGCEPCTRPVLP
Sbjct: 241 PVFEGLDGGIGSLVKWNPVANVGSKDIWDFLRSMNVPVNTLHSQGYVSIGCEPCTRPVLP 300

Query: 301 WQHEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTG 360
           WQHEREGRWWWED+ AKECGLHKGNLKQ D  QLNG+ NG     DIF SQNLV+L R G
Sbjct: 301 WQHEREGRWWWEDANAKECGLHKGNLKQEDPAQLNGDANGISTDADIFESQNLVSLTRGG 360

Query: 361 IENLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFA 420
           IENLARL  RKEPW+VVLYAPWC+FCQAMEGSYVEL EKLAG+GVKVGKFRADGE+KEFA
Sbjct: 361 IENLARLEGRKEPWIVVLYAPWCRFCQAMEGSYVELAEKLAGTGVKVGKFRADGEEKEFA 420

Query: 421 KAELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 458
           + ELQLGSFPTILFFPKHSS+ IKYPSEKRDVDSLM FVNA R
Sbjct: 421 QQELQLGSFPTILFFPKHSSRPIKYPSEKRDVDSLMAFVNAFR 463

BLAST of Cp4.1LG20g03620 vs. NCBI nr
Match: gi|703090257|ref|XP_010094030.1| (5'-adenylylsulfate reductase 1 [Morus notabilis])

HSP 1 Score: 742.7 bits (1916), Expect = 4.0e-211
Identity = 359/445 (80.67%), Postives = 392/445 (88.09%), Query Frame = 1

Query: 15  AVSSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPINSQSQRNNLFVPLAVNTLASE 74
           + SS+ KA +IGSV+LL   H  SS +N  +R   +KP+N++ +RN++ VP A   +A E
Sbjct: 22  SASSELKASQIGSVRLLERSHGQSSALNISQRKSAVKPLNAEPKRNDMIVPFAATAIAPE 81

Query: 75  VVEK--AETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFSGAEDVALIEYARLTGR 134
           V EK   E  DY+ LAK LENASPLEIMD+ALE+FGNDIAIAFSGAEDV LIEYA LTGR
Sbjct: 82  VEEKEKVEAEDYEQLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVVLIEYAYLTGR 141

Query: 135 PFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFYEDGHQE 194
           PFRVFSLDTGRLN ETY+FFDAVEKHYGI IEYMFPDAVEVQ LVR+KGLFSFYEDGHQE
Sbjct: 142 PFRVFSLDTGRLNPETYRFFDAVEKHYGIRIEYMFPDAVEVQALVRSKGLFSFYEDGHQE 201

Query: 195 CCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGSLIKWNP 254
           CCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGSL+KWNP
Sbjct: 202 CCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPVFEGLDGGIGSLVKWNP 261

Query: 255 VANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQHEREGRWWWEDSKAKE 314
           VANV+G DIWNFLRTM+VPVNSLHSQGYVSIGCEPCTRPVLP QHEREGRWWWED+KAKE
Sbjct: 262 VANVNGVDIWNFLRTMDVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWEDAKAKE 321

Query: 315 CGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIENLARLGNRKEPWLVVL 374
           CGLHKGN+KQ D  QLN NGNG     DIF SQNLV L+R GIENLA+LG+RKEPW+VVL
Sbjct: 322 CGLHKGNIKQEDTAQLNSNGNGAAAETDIFNSQNLVTLSRAGIENLAKLGSRKEPWIVVL 381

Query: 375 YAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKAELQLGSFPTILFFPKH 434
           YAPWCQFCQAMEGSY+EL +KLAGSGVKVGKFRADGEQKE+AKAELQL SFPTILFFP H
Sbjct: 382 YAPWCQFCQAMEGSYLELADKLAGSGVKVGKFRADGEQKEYAKAELQLQSFPTILFFPGH 441

Query: 435 SSKAIKYPSEKRDVDSLMGFVNALR 458
           S++ IKYPSEKRDVDSLM FVNALR
Sbjct: 442 SAQPIKYPSEKRDVDSLMAFVNALR 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
APR1_ARATH2.0e-19471.585'-adenylylsulfate reductase 1, chloroplastic OS=Arabidopsis thaliana GN=APR1 PE... [more]
APR3_ARATH2.4e-19273.655'-adenylylsulfate reductase 3, chloroplastic OS=Arabidopsis thaliana GN=APR3 PE... [more]
APR2_ARATH2.1e-18871.245'-adenylylsulfate reductase 2, chloroplastic OS=Arabidopsis thaliana GN=APR2 PE... [more]
APR1_ORYSJ9.8e-17866.67Probable 5'-adenylylsulfate reductase 1, chloroplastic OS=Oryza sativa subsp. ja... [more]
CYSH_PSEAE3.0e-7853.72Phosphoadenosine phosphosulfate reductase OS=Pseudomonas aeruginosa (strain ATCC... [more]
Match NameE-valueIdentityDescription
E5GC59_CUCME1.9e-23688.57Adenylyl-sulfate reductase OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0LUS5_CUCSA2.1e-23586.93Uncharacterized protein OS=Cucumis sativus GN=Csa_1G062380 PE=4 SV=1[more]
A0A0A0KHT4_CUCSA1.7e-21378.62Uncharacterized protein OS=Cucumis sativus GN=Csa_6G513670 PE=4 SV=1[more]
W9QV50_9ROSA2.8e-21180.675'-adenylylsulfate reductase 1 OS=Morus notabilis GN=L484_018046 PE=4 SV=1[more]
A0A067JAD3_JATCU8.9e-21078.41Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21207 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G04610.11.1e-19571.58 APS reductase 1[more]
AT4G21990.11.4e-19373.65 APS reductase 3[more]
AT1G62180.11.2e-18971.24 5'adenylylphosphosulfate reductase 2[more]
AT5G60640.13.3e-0631.19 PDI-like 1-4[more]
AT3G54960.17.4e-0644.44 PDI-like 1-3[more]
Match NameE-valueIdentityDescription
gi|659067724|ref|XP_008440977.1|2.7e-23688.57PREDICTED: 5'-adenylylsulfate reductase 1, chloroplastic [Cucumis melo][more]
gi|778658388|ref|XP_011652629.1|3.0e-23586.93PREDICTED: 5'-adenylylsulfate reductase 1, chloroplastic [Cucumis sativus][more]
gi|659078978|ref|XP_008440009.1|3.8e-21478.66PREDICTED: 5'-adenylylsulfate reductase 1, chloroplastic-like [Cucumis melo][more]
gi|449433968|ref|XP_004134768.1|2.5e-21378.62PREDICTED: 5'-adenylylsulfate reductase 1, chloroplastic [Cucumis sativus][more]
gi|703090257|ref|XP_010094030.1|4.0e-21180.675'-adenylylsulfate reductase 1 [Morus notabilis][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0045454cell redox homeostasis
GO:0055114oxidation-reduction process
GO:0019419sulfate reduction
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0016671oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR014729Rossmann-like_a/b/a_fold
IPR013766Thioredoxin_domain
IPR012336Thioredoxin-like_fold
IPR004508Thioredoxin-indep_APS_Rdtase
IPR002500PAPS_reduct
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045454 cell redox homeostasis
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0019379 sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)
biological_process GO:0008152 metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0019419 sulfate reduction
cellular_component GO:0005623 cell
cellular_component GO:0005575 cellular_component
molecular_function GO:0004604 phosphoadenylyl-sulfate reductase (thioredoxin) activity
molecular_function GO:0033741 adenylyl-sulfate reductase (glutathione) activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016671 oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g03620.1Cp4.1LG20g03620.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002500Phosphoadenosine phosphosulphate reductasePFAMPF01507PAPS_reductcoord: 111..291
score: 3.8
IPR004508Thioredoxin-independent 5'-adenylylsulphate reductaseTIGRFAMsTIGR00424TIGR00424coord: 5..457
score: 8.7E
IPR012336Thioredoxin-like foldGENE3DG3DSA:3.40.30.10coord: 357..455
score: 9.5
IPR012336Thioredoxin-like foldunknownSSF52833Thioredoxin-likecoord: 346..454
score: 5.0
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 362..453
score: 8.8
IPR013766Thioredoxin domainPROFILEPS51352THIOREDOXIN_2coord: 336..457
score: 10
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 76..287
score: 6.3
NoneNo IPR availablePANTHERPTHR23293FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASEcoord: 1..346
score: 2.1E
NoneNo IPR availablePANTHERPTHR23293:SF11PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASEcoord: 1..346
score: 2.1E
NoneNo IPR availableunknownSSF52402Adenine nucleotide alpha hydrolases-likecoord: 84..279
score: 2.65

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG20g03620Cucsa.045160Cucumber (Gy14) v1cgycpeB0040
Cp4.1LG20g03620Cucsa.085750Cucumber (Gy14) v1cgycpeB0159
Cp4.1LG20g03620CmaCh18G002660Cucurbita maxima (Rimu)cmacpeB442
Cp4.1LG20g03620CmaCh03G005430Cucurbita maxima (Rimu)cmacpeB677
Cp4.1LG20g03620CmaCh13G007450Cucurbita maxima (Rimu)cmacpeB234
Cp4.1LG20g03620CmoCh13G007760Cucurbita moschata (Rifu)cmocpeB201
Cp4.1LG20g03620CmoCh03G005660Cucurbita moschata (Rifu)cmocpeB631
Cp4.1LG20g03620CmoCh18G002660Cucurbita moschata (Rifu)cmocpeB403
Cp4.1LG20g03620Cla019374Watermelon (97103) v1cpewmB503
Cp4.1LG20g03620Cla014443Watermelon (97103) v1cpewmB513
Cp4.1LG20g03620Csa1G062380Cucumber (Chinese Long) v2cpecuB506
Cp4.1LG20g03620Csa6G513670Cucumber (Chinese Long) v2cpecuB525
Cp4.1LG20g03620MELO3C002450Melon (DHL92) v3.5.1cpemeB477
Cp4.1LG20g03620MELO3C007398Melon (DHL92) v3.5.1cpemeB485
Cp4.1LG20g03620ClCG01G021900Watermelon (Charleston Gray)cpewcgB450
Cp4.1LG20g03620ClCG03G004660Watermelon (Charleston Gray)cpewcgB463
Cp4.1LG20g03620CSPI06G33480Wild cucumber (PI 183967)cpecpiB527
Cp4.1LG20g03620CSPI01G10620Wild cucumber (PI 183967)cpecpiB506
Cp4.1LG20g03620Lsi01G004340Bottle gourd (USVL1VR-Ls)cpelsiB415
Cp4.1LG20g03620Lsi02G005960Bottle gourd (USVL1VR-Ls)cpelsiB417
Cp4.1LG20g03620MELO3C007398.2Melon (DHL92) v3.6.1cpemedB573
Cp4.1LG20g03620MELO3C002450.2Melon (DHL92) v3.6.1cpemedB559
Cp4.1LG20g03620CsaV3_1G010690Cucumber (Chinese Long) v3cpecucB0624
Cp4.1LG20g03620CsaV3_6G048960Cucumber (Chinese Long) v3cpecucB0650
Cp4.1LG20g03620Bhi08G001498Wax gourdcpewgoB0637
Cp4.1LG20g03620Bhi03G000451Wax gourdcpewgoB0632
Cp4.1LG20g03620CsGy6G033030Cucumber (Gy14) v2cgybcpeB814
Cp4.1LG20g03620CsGy1G010500Cucumber (Gy14) v2cgybcpeB070
Cp4.1LG20g03620Carg07552Silver-seed gourdcarcpeB1401
Cp4.1LG20g03620Carg06844Silver-seed gourdcarcpeB0697
Cp4.1LG20g03620Carg13690Silver-seed gourdcarcpeB1291
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG20g03620Cp4.1LG09g09190Cucurbita pepo (Zucchini)cpecpeB048
Cp4.1LG20g03620Cp4.1LG10g09550Cucurbita pepo (Zucchini)cpecpeB086
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG20g03620Cucurbita pepo (Zucchini)cpecpeB412
Cp4.1LG20g03620Cucurbita maxima (Rimu)cmacpeB881
Cp4.1LG20g03620Cucurbita moschata (Rifu)cmocpeB818
Cp4.1LG20g03620Cucumber (Chinese Long) v2cpecuB526
Cp4.1LG20g03620Silver-seed gourdcarcpeB0916
Cp4.1LG20g03620Wax gourdcpewgoB0655