Cp4.1LG18g02780 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG18g02780
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
LocationCp4.1LG18 : 4456209 .. 4456814 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTCCCTTTCTCTCCTCCTCTTCCTCCTCCTCTCTCTCTCCCTCTCCGCCGCAGCCCACGACGGCCCTCCGCCGTCCCAAGATCTGATCCACTCCTCCTGCCTCCAAGCCAGCTACCCAAGTCTCTGCATCAGAACCCTCTCCTCCTACTCTGGCGCTGTCAACACCCCACAAGATCTCGCCCAAGCCACCCTCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCAATTCCTCCGCCAACCTCCGGCAGGCCTCGAAGCGGCAACGCGCGGCGGTCGACGACTGCGTGGAACAGATTGGAGACTCTGTTGAGGAGCTGAGCAACACTCTGGGTCTCCTCCGCCACCTCCCCTACGGCGACCGCCGGTCTTTCCGATTGCAAATGAGCAATGCCCAGACGTGGGTCAGCGCCGCCTTGACCAATGAAGACACGTGTCTCGATGGGTTTAAGGAAGTGGATGGGGAGGTGAAATTTGATGTGAAGAAGAGGATTTCTAAGGTCGCTAAAGCTACTAGTAATGCTCTGTATATGATTAATCGCCTCGACGTCGGAACCCCCGCCGGGAGGAAGAAATTGGCCGCTCCTTGA

mRNA sequence

ATGACTTCCCTTTCTCTCCTCCTCTTCCTCCTCCTCTCTCTCTCCCTCTCCGCCGCAGCCCACGACGGCCCTCCGCCGTCCCAAGATCTGATCCACTCCTCCTGCCTCCAAGCCAGCTACCCAAGTCTCTGCATCAGAACCCTCTCCTCCTACTCTGGCGCTGTCAACACCCCACAAGATCTCGCCCAAGCCACCCTCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCAATTCCTCCGCCAACCTCCGGCAGGCCTCGAAGCGGCAACGCGCGGCGGTCGACGACTGCGTGGAACAGATTGGAGACTCTGTTGAGGAGCTGAGCAACACTCTGGGTCTCCTCCGCCACCTCCCCTACGGCGACCGCCGGTCTTTCCGATTGCAAATGAGCAATGCCCAGACGTGGGTCAGCGCCGCCTTGACCAATGAAGACACGTGTCTCGATGGGTTTAAGGAAGTGGATGGGGAGGTGAAATTTGATGTGAAGAAGAGGATTTCTAAGGTCGCTAAAGCTACTAGTAATGCTCTGTATATGATTAATCGCCTCGACGTCGGAACCCCCGCCGGGAGGAAGAAATTGGCCGCTCCTTGA

Coding sequence (CDS)

ATGACTTCCCTTTCTCTCCTCCTCTTCCTCCTCCTCTCTCTCTCCCTCTCCGCCGCAGCCCACGACGGCCCTCCGCCGTCCCAAGATCTGATCCACTCCTCCTGCCTCCAAGCCAGCTACCCAAGTCTCTGCATCAGAACCCTCTCCTCCTACTCTGGCGCTGTCAACACCCCACAAGATCTCGCCCAAGCCACCCTCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCAATTCCTCCGCCAACCTCCGGCAGGCCTCGAAGCGGCAACGCGCGGCGGTCGACGACTGCGTGGAACAGATTGGAGACTCTGTTGAGGAGCTGAGCAACACTCTGGGTCTCCTCCGCCACCTCCCCTACGGCGACCGCCGGTCTTTCCGATTGCAAATGAGCAATGCCCAGACGTGGGTCAGCGCCGCCTTGACCAATGAAGACACGTGTCTCGATGGGTTTAAGGAAGTGGATGGGGAGGTGAAATTTGATGTGAAGAAGAGGATTTCTAAGGTCGCTAAAGCTACTAGTAATGCTCTGTATATGATTAATCGCCTCGACGTCGGAACCCCCGCCGGGAGGAAGAAATTGGCCGCTCCTTGA

Protein sequence

MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQDLAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNALYMINRLDVGTPAGRKKLAAP
BLAST of Cp4.1LG18g02780 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.7e-26
Identity = 69/186 (37.10%), Postives = 115/186 (61.83%), Query Frame = 1

Query: 3   SLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAV-NTPQDL 62
           S S L+F  L + L+AA+     P+   I +SC   +YP++C ++LS+Y+  + N PQ+L
Sbjct: 4   SKSTLVFSALLVILAAAS---AAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQEL 63

Query: 63  AQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRH 122
           A   L VSL+  +    ++   +   +    RQ AA+ DC+E++ DS++ +S +   +++
Sbjct: 64  ASTALQVSLTRTQQAQTFMKRLN-KFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKN 123

Query: 123 LPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGF--KEVDGEVKFDVKKRISKVAKATSN 182
           L +     F  +MSN +TWVSAALT+E TC+DGF  K +DG++K  V+ ++  VA+ TSN
Sbjct: 124 LSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSN 183

Query: 183 ALYMIN 186
           AL ++N
Sbjct: 184 ALALVN 185

BLAST of Cp4.1LG18g02780 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 7.5e-13
Identity = 53/193 (27.46%), Postives = 103/193 (53.37%), Query Frame = 1

Query: 14  LSLSAAAHDGPPPSQDLIHSSCLQASYPSLC---IRTLSSYSGAVNTPQDLAQATLSVSL 73
           +++S++AH        ++ S+C    +P LC   I  +S +S  V + +D+ + +L++++
Sbjct: 56  MAISSSAHA-------IVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITV 115

Query: 74  SLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSF 133
              R     +       +  + R++ A+ DC+E + ++++EL   +  L   P  +++S 
Sbjct: 116 KAVRRNYYAVKELIKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYP--NKKSL 175

Query: 134 RLQMSNAQTWVSAALTNEDTCLDGFK--EVDGEVKFDVKKRISKVAKATSNALYMINRLD 193
           +  + + +T +S+A+TN++TCLDGF   E D +V+  + K    V K  SNAL MI  + 
Sbjct: 176 KEHVEDLKTLISSAITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMT 235

Query: 194 VGTPAGRKKLAAP 202
               A   KL+AP
Sbjct: 236 DTDIANEMKLSAP 239

BLAST of Cp4.1LG18g02780 vs. Swiss-Prot
Match: PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.2e-12
Identity = 50/154 (32.47%), Postives = 82/154 (53.25%), Query Frame = 1

Query: 31  IHSSCLQASYPSLCIRTLSSYSGAVNTPQDLAQATLSVSLSLARDLSRYLSNSSANLRQA 90
           I  +C ++ YP+LCI TL  + G++ T  +     +S + +L +      ++S+    Q 
Sbjct: 75  ISRTCSKSLYPNLCIDTLLDFPGSL-TADENELIHISFNATLQKFSKALYTSSTITYTQM 134

Query: 91  SKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALTNEDT 150
             R R+A D C+E + DSV+ L+  L  +  +  GD        S+  TW+S+A+TN DT
Sbjct: 135 PPRVRSAYDSCLELLDDSVDALTRALSSVVVVS-GDESH-----SDVMTWLSSAMTNHDT 194

Query: 151 CLDGFKEVD---GEVKFDVKKRISKVAKATSNAL 182
           C DGF E++   GEVK  V   +  +++  SN L
Sbjct: 195 CTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCL 221

BLAST of Cp4.1LG18g02780 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 2.8e-12
Identity = 56/190 (29.47%), Postives = 98/190 (51.58%), Query Frame = 1

Query: 7   LLFLLLSLSLSAAAHDG------PPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQ- 66
           LLF+     +SA A          P S  ++ SSC    YP LCI  + +  G   T Q 
Sbjct: 29  LLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQK 88

Query: 67  DLAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLL 126
           D+ +A+++++++        +       +  + R++ A+ DC+E I ++++EL  T+  L
Sbjct: 89  DVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETIDETLDELHETVEDL 148

Query: 127 RHLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFK--EVDGEVKFDVKKRISKVAKAT 186
            HL Y  +++ R    + +T +S+A+TN++TCLDGF   + D +V+  + K    V    
Sbjct: 149 -HL-YPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMC 208

Query: 187 SNALYMINRL 188
           SNAL MI  +
Sbjct: 209 SNALAMIKNM 216

BLAST of Cp4.1LG18g02780 vs. Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.1e-11
Identity = 53/159 (33.33%), Postives = 80/159 (50.31%), Query Frame = 1

Query: 26  PSQDLIHSSCLQASYPSLCIRTLSSYSG--AVNTPQDLAQATLSVSLSLARDLSRYLSNS 85
           PSQ  I  +C    +P LC+ +L  + G  A ++ +DL   T  V+++L        S++
Sbjct: 83  PSQ-AISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVT--VNMTLHHFSHALYSSA 142

Query: 86  SANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSA 145
           S +      R R+A D CVE + DSV+ LS  L  +         S   +  +  TW+SA
Sbjct: 143 SLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSV--------VSSSAKPQDVTTWLSA 202

Query: 146 ALTNEDTCLDGFKEV-DGEVKFDVKKRISKVAKATSNAL 182
           ALTN DTC +GF  V DG VK  +   +  +++  SN L
Sbjct: 203 ALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCL 230

BLAST of Cp4.1LG18g02780 vs. TrEMBL
Match: A0A0A0LWR8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G597770 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.3e-72
Identity = 157/199 (78.89%), Postives = 173/199 (86.93%), Query Frame = 1

Query: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT LSLLL LL SLSLSAA       SQDLIHSSCLQASYP+LCIRTLSSY+GAV TP+D
Sbjct: 28  MTVLSLLL-LLFSLSLSAAHGGRGAISQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRD 87

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQAT+SVSLSLA++LS YLS+S   LRQAS++QRAAVDDCV+QIGDSVEELSNTLG+LR
Sbjct: 88  LAQATISVSLSLAQNLSEYLSDS---LRQASRQQRAAVDDCVDQIGDSVEELSNTLGVLR 147

Query: 121 HLPYGD-RRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180
           HLP GD RR FRL+M NA+TWVSAALTNE+TCLDGFKEVDGEVK DVK+RI KVAK TSN
Sbjct: 148 HLPCGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRILKVAKVTSN 207

Query: 181 ALYMINRLDVGTPAGRKKL 199
           AL+MINRLD G P GRK L
Sbjct: 208 ALFMINRLDGGNPTGRKML 222

BLAST of Cp4.1LG18g02780 vs. TrEMBL
Match: A0A061GSW0_THECC (Plant invertase/pectin methylesterase inhibitor superfamily protein OS=Theobroma cacao GN=TCM_037478 PE=4 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 4.8e-51
Identity = 117/188 (62.23%), Postives = 138/188 (73.40%), Query Frame = 1

Query: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT++   L     L LS+AA       +DL+ SSC+ ASYPSLC+RTLSSYSG  NTP+D
Sbjct: 1   MTTIPFFLLTTSLLCLSSAAASKQDAPRDLVRSSCVHASYPSLCLRTLSSYSGPANTPRD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQA + VSLS AR +S YL+ S       SKR+R A+ DCVEQI +SVEELS TLG L+
Sbjct: 61  LAQAAVKVSLSRARKVSNYLTTSVTG---KSKRERGALSDCVEQIAESVEELSRTLGELK 120

Query: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180
           HL      +F  QMSNAQTWVSAALTNEDTCLDGF+ VDG+VK DVK++I+ VAK TSNA
Sbjct: 121 HLR---GETFEFQMSNAQTWVSAALTNEDTCLDGFEGVDGKVKSDVKRKITNVAKVTSNA 180

Query: 181 LYMINRLD 189
           LYMINRLD
Sbjct: 181 LYMINRLD 182

BLAST of Cp4.1LG18g02780 vs. TrEMBL
Match: F6HGM6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0016g00590 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-49
Identity = 106/162 (65.43%), Postives = 132/162 (81.48%), Query Frame = 1

Query: 27  SQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQDLAQATLSVSLSLARDLSRYLSNSSAN 86
           ++DL+HSSC+ ASYP++C+RTLSSYSG   TP+DLAQA + VSLS A+ +S YL N  A 
Sbjct: 21  AEDLVHSSCIHASYPNICLRTLSSYSGPAKTPRDLAQAAVKVSLSRAQRVSTYL-NGVAG 80

Query: 87  LRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALT 146
             +ASKR++ A+ DCVEQI D+VEELSNTL  L+HL  G   +FR QMSNA+TWVSAALT
Sbjct: 81  QGKASKREQVALSDCVEQISDTVEELSNTLKELKHLRPG---TFRWQMSNAETWVSAALT 140

Query: 147 NEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNALYMINRLD 189
           NEDTCLDGF+ VDG+VK DVK++I+ VA+ TSNALY+INRLD
Sbjct: 141 NEDTCLDGFQGVDGKVKADVKRKITNVARVTSNALYLINRLD 178

BLAST of Cp4.1LG18g02780 vs. TrEMBL
Match: A0A067KCU5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07533 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.3e-48
Identity = 116/199 (58.29%), Postives = 148/199 (74.37%), Query Frame = 1

Query: 7   LLFLLLSLSLS----AAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQDLA 66
           +LFL LSLSL+     A H   P  QDL+ SSCL ASYP LCIRTLSS SG+ NTP+D+A
Sbjct: 13  ILFLFLSLSLTRYSATANHHNAP--QDLVRSSCLHASYPDLCIRTLSSCSGSANTPRDIA 72

Query: 67  QATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHL 126
           QA ++VS+  AR +S+ L+  SA L+  +KR+R A+ DCVEQI D+V+ELS+TL  L+HL
Sbjct: 73  QAAVNVSVERARGVSKNLNALSAGLK--TKRERVALSDCVEQISDTVDELSSTLSELKHL 132

Query: 127 PYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNALY 186
                 +F+ QMSNA+TWVSAALTNE+TCLDGF+EV+ +VK DVK++I+ VA+ TSNALY
Sbjct: 133 R---GETFQWQMSNAETWVSAALTNENTCLDGFEEVESKVKGDVKRQITNVARVTSNALY 192

Query: 187 MINRLDVGTPAGRKKLAAP 202
           MINRLD     GR +L  P
Sbjct: 193 MINRLD--ESRGRSRLVTP 202

BLAST of Cp4.1LG18g02780 vs. TrEMBL
Match: B9RR57_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_0710110 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 2.9e-48
Identity = 112/188 (59.57%), Postives = 139/188 (73.94%), Query Frame = 1

Query: 1   MTSLSLLLFLLLSL-SLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQ 60
           M  L  L  L LSL S +A  H      QDL+HSSCL ASYP+LC+RTLSSYSG  NTP 
Sbjct: 65  MPPLFSLFILFLSLCSAAAKTHSAGAAPQDLVHSSCLHASYPNLCMRTLSSYSGPANTPH 124

Query: 61  DLAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLL 120
           DLA A + VSL  A  +S+YLS+ S+ L+  ++++R A+ DCVEQI DSV+ELSNTL  L
Sbjct: 125 DLALAAVEVSLGRASKVSKYLSSLSSGLK--TRKERVALSDCVEQISDSVDELSNTLNEL 184

Query: 121 RHLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180
           +HL      +FR QM+NAQTWVSAALTNE+TCLDGF++V  +VK DVK++I+ VA+ TSN
Sbjct: 185 KHLR---GETFRWQMNNAQTWVSAALTNEETCLDGFEQVQRKVKCDVKRKITNVARVTSN 244

Query: 181 ALYMINRL 188
           ALYMINRL
Sbjct: 245 ALYMINRL 247

BLAST of Cp4.1LG18g02780 vs. TAIR10
Match: AT5G20740.1 (AT5G20740.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 172.6 bits (436), Expect = 2.5e-43
Identity = 96/189 (50.79%), Postives = 139/189 (73.54%), Query Frame = 1

Query: 4   LSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSG-AVNTPQDLA 63
           +++   ++ + S     H+G   ++D++HSSC  ASYPSLC+RTLSSYSG  +   +DLA
Sbjct: 10  VAIAFAVIFTASTVHGRHNG---AEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLA 69

Query: 64  QATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHL 123
           QA + +SLS A+  ++ L+    ++ +  K+++AA+ DCVE IGDSV+ELS TLG+L+HL
Sbjct: 70  QAAIKISLSHAQSAAKKLAVVRDSVGK-KKQEKAALVDCVEMIGDSVDELSRTLGVLKHL 129

Query: 124 PY--GDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEV-DGEVKFDVKKRISKVAKATSN 183
               G  + FR QMSNAQTW SAALT++DTCLDGF+ + DGE+K +VK+ ++KVA+ TSN
Sbjct: 130 RVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSN 189

Query: 184 ALYMINRLD 189
           ALYM+N+LD
Sbjct: 190 ALYMVNQLD 194

BLAST of Cp4.1LG18g02780 vs. TAIR10
Match: AT1G62770.1 (AT1G62770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 119.8 bits (299), Expect = 2.0e-27
Identity = 72/189 (38.10%), Postives = 117/189 (61.90%), Query Frame = 1

Query: 5   SLLLFLLLSLSL--SAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAV--NTPQD 64
           ++ L +LLS+++  S++A      S   I SSC    YPSLC+ TLS+Y+  +  N  QD
Sbjct: 6   TIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQD 65

Query: 65  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 124
           LAQ  L +SL+ A+ ++ +++  +    +  +R+  A+ DC+E +G+SV+ L+ ++  L 
Sbjct: 66  LAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELA 125

Query: 125 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKE--VDGEVKFDVKKRISKVAKAT 184
              +      F  +MSN QTWVSAALT+E TCLDGF E  + G+VK  ++ ++  VA+ T
Sbjct: 126 RAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVT 185

Query: 185 SNALYMINR 187
           SNAL ++N+
Sbjct: 186 SNALALVNQ 194

BLAST of Cp4.1LG18g02780 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 119.8 bits (299), Expect = 2.0e-27
Identity = 79/190 (41.58%), Postives = 117/190 (61.58%), Query Frame = 1

Query: 4   LSLLLFLL-LSLSLSAAAHDGPPPSQ---DLIHSSCLQASYPSLCIRTLSSYSGAVNT-P 63
           LSL+LF+L LS +    A +    S    + I +SC   SYP  C ++LSSY+  +   P
Sbjct: 7   LSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQP 66

Query: 64  QDLAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGL 123
           + LA+  L+VS++ A+    Y+S  + + +  +KRQ  AV DC+E++GD+V+ LSN+L  
Sbjct: 67  RKLAETALAVSIARAKSAKTYVSEMT-DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKE 126

Query: 124 LRHLPYGDR-RSFRLQMSNAQTWVSAALTNEDTCLDGF--KEVDGEVKFDVKKRISKVAK 183
           L+HL  GD    F   +SN +TW SAALT+E  C+DGF  K + GE+K  ++  I  VA+
Sbjct: 127 LKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAE 186

Query: 184 ATSNALYMIN 186
            TSNAL +IN
Sbjct: 187 ETSNALALIN 195

BLAST of Cp4.1LG18g02780 vs. TAIR10
Match: AT1G23205.1 (AT1G23205.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 119.8 bits (299), Expect = 2.0e-27
Identity = 82/190 (43.16%), Postives = 110/190 (57.89%), Query Frame = 1

Query: 6   LLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAV-NTPQDLAQA 65
           L  FL   L L    H     S D I +SC    YP LC  +LSS+S +V N P  LA+A
Sbjct: 10  LFFFLASVLPLILTVHSQSDDS-DFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLARA 69

Query: 66  TLSVSLSLARDLSRYLSNSS-----ANLRQASKRQRAAV-DDCVEQIGDSVEELSNTLGL 125
            +SV+L+   DL+ YL+N +     +N   A     AAV  DC + + D+VEE+  ++  
Sbjct: 70  AISVTLTKTLDLASYLANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMKQ 129

Query: 126 LRHL-PYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEV-KFDVKKRISKVAKA 185
           +R L   G   SFR QMSN QTW+SAALT+E+TC DGFK++  E  K D+  R+  V K 
Sbjct: 130 MRELVSTGSLESFRFQMSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKM 189

Query: 186 TSNALYMINR 187
           TSNAL ++NR
Sbjct: 190 TSNALALVNR 198

BLAST of Cp4.1LG18g02780 vs. TAIR10
Match: AT1G62760.1 (AT1G62760.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 116.7 bits (291), Expect = 1.7e-26
Identity = 74/194 (38.14%), Postives = 109/194 (56.19%), Query Frame = 1

Query: 14  LSLSAAAHDGPPPSQ---------------------DLIHSSCLQASYPSLCIRTLSSYS 73
           LSLS ++   PPPS                      D I +SC    Y ++C  +LS Y+
Sbjct: 110 LSLSPSSPPPPPPSSSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYA 169

Query: 74  GAVNT-PQDLAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEE 133
             + + PQ LA   L+++LS A+  S+++ N S      ++ +  AV DCVE+IGDSV  
Sbjct: 170 STIRSNPQKLAVIALNLTLSSAKSASKFVKNISHG-GGLTRLEVVAVADCVEEIGDSVTS 229

Query: 134 LSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRIS 186
           L +++  L  + Y D   F + MS+ +TWVSAALTN+DTC+DGF  V   VK  V++ + 
Sbjct: 230 LQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGFSLVKTAVKDLVRRHVV 289

BLAST of Cp4.1LG18g02780 vs. NCBI nr
Match: gi|449452771|ref|XP_004144132.1| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 280.8 bits (717), Expect = 1.9e-72
Identity = 157/199 (78.89%), Postives = 173/199 (86.93%), Query Frame = 1

Query: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT LSLLL LL SLSLSAA       SQDLIHSSCLQASYP+LCIRTLSSY+GAV TP+D
Sbjct: 1   MTVLSLLL-LLFSLSLSAAHGGRGAISQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQAT+SVSLSLA++LS YLS+S   LRQAS++QRAAVDDCV+QIGDSVEELSNTLG+LR
Sbjct: 61  LAQATISVSLSLAQNLSEYLSDS---LRQASRQQRAAVDDCVDQIGDSVEELSNTLGVLR 120

Query: 121 HLPYGD-RRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180
           HLP GD RR FRL+M NA+TWVSAALTNE+TCLDGFKEVDGEVK DVK+RI KVAK TSN
Sbjct: 121 HLPCGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRILKVAKVTSN 180

Query: 181 ALYMINRLDVGTPAGRKKL 199
           AL+MINRLD G P GRK L
Sbjct: 181 ALFMINRLDGGNPTGRKML 195

BLAST of Cp4.1LG18g02780 vs. NCBI nr
Match: gi|700211234|gb|KGN66330.1| (hypothetical protein Csa_1G597770 [Cucumis sativus])

HSP 1 Score: 280.8 bits (717), Expect = 1.9e-72
Identity = 157/199 (78.89%), Postives = 173/199 (86.93%), Query Frame = 1

Query: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT LSLLL LL SLSLSAA       SQDLIHSSCLQASYP+LCIRTLSSY+GAV TP+D
Sbjct: 28  MTVLSLLL-LLFSLSLSAAHGGRGAISQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRD 87

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQAT+SVSLSLA++LS YLS+S   LRQAS++QRAAVDDCV+QIGDSVEELSNTLG+LR
Sbjct: 88  LAQATISVSLSLAQNLSEYLSDS---LRQASRQQRAAVDDCVDQIGDSVEELSNTLGVLR 147

Query: 121 HLPYGD-RRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180
           HLP GD RR FRL+M NA+TWVSAALTNE+TCLDGFKEVDGEVK DVK+RI KVAK TSN
Sbjct: 148 HLPCGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRILKVAKVTSN 207

Query: 181 ALYMINRLDVGTPAGRKKL 199
           AL+MINRLD G P GRK L
Sbjct: 208 ALFMINRLDGGNPTGRKML 222

BLAST of Cp4.1LG18g02780 vs. NCBI nr
Match: gi|659100173|ref|XP_008450965.1| (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 274.6 bits (701), Expect = 1.3e-70
Identity = 150/197 (76.14%), Postives = 170/197 (86.29%), Query Frame = 1

Query: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT LSLLL LLLSLSLSAA   G   SQDLI SSCLQA YP+LCIRTLSSY+G+V TP+D
Sbjct: 1   MTVLSLLLLLLLSLSLSAAHGGGGAVSQDLIRSSCLQARYPTLCIRTLSSYAGSVKTPRD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQ T+SVSLSLA++LS YLS+S   LR+AS++QRAAVDDCV+QIGDSVEELSNTLG+LR
Sbjct: 61  LAQVTISVSLSLAQNLSEYLSDS---LRKASRQQRAAVDDCVDQIGDSVEELSNTLGVLR 120

Query: 121 HLPYGD-RRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180
           HLP GD RR FRL+M NA+TWVSAALTNE+TCLDGFKEVDGEVK DVK+RI KVAK TSN
Sbjct: 121 HLPCGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRIVKVAKVTSN 180

Query: 181 ALYMINRLDVGTPAGRK 197
           AL+MINRLD G   G++
Sbjct: 181 ALFMINRLDSGNSTGKE 194

BLAST of Cp4.1LG18g02780 vs. NCBI nr
Match: gi|590574990|ref|XP_007012560.1| (Plant invertase/pectin methylesterase inhibitor superfamily protein [Theobroma cacao])

HSP 1 Score: 209.1 bits (531), Expect = 6.9e-51
Identity = 117/188 (62.23%), Postives = 138/188 (73.40%), Query Frame = 1

Query: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60
           MT++   L     L LS+AA       +DL+ SSC+ ASYPSLC+RTLSSYSG  NTP+D
Sbjct: 1   MTTIPFFLLTTSLLCLSSAAASKQDAPRDLVRSSCVHASYPSLCLRTLSSYSGPANTPRD 60

Query: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120
           LAQA + VSLS AR +S YL+ S       SKR+R A+ DCVEQI +SVEELS TLG L+
Sbjct: 61  LAQAAVKVSLSRARKVSNYLTTSVTG---KSKRERGALSDCVEQIAESVEELSRTLGELK 120

Query: 121 HLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNA 180
           HL      +F  QMSNAQTWVSAALTNEDTCLDGF+ VDG+VK DVK++I+ VAK TSNA
Sbjct: 121 HLR---GETFEFQMSNAQTWVSAALTNEDTCLDGFEGVDGKVKSDVKRKITNVAKVTSNA 180

Query: 181 LYMINRLD 189
           LYMINRLD
Sbjct: 181 LYMINRLD 182

BLAST of Cp4.1LG18g02780 vs. NCBI nr
Match: gi|225444639|ref|XP_002276089.1| (PREDICTED: 21 kDa protein [Vitis vinifera])

HSP 1 Score: 203.8 bits (517), Expect = 2.9e-49
Identity = 106/162 (65.43%), Postives = 132/162 (81.48%), Query Frame = 1

Query: 27  SQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQDLAQATLSVSLSLARDLSRYLSNSSAN 86
           ++DL+HSSC+ ASYP++C+RTLSSYSG   TP+DLAQA + VSLS A+ +S YL N  A 
Sbjct: 22  AEDLVHSSCIHASYPNICLRTLSSYSGPAKTPRDLAQAAVKVSLSRAQRVSTYL-NGVAG 81

Query: 87  LRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALT 146
             +ASKR++ A+ DCVEQI D+VEELSNTL  L+HL  G   +FR QMSNA+TWVSAALT
Sbjct: 82  QGKASKREQVALSDCVEQISDTVEELSNTLKELKHLRPG---TFRWQMSNAETWVSAALT 141

Query: 147 NEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNALYMINRLD 189
           NEDTCLDGF+ VDG+VK DVK++I+ VA+ TSNALY+INRLD
Sbjct: 142 NEDTCLDGFQGVDGKVKADVKRKITNVARVTSNALYLINRLD 179

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
21KD_DAUCA2.7e-2637.1021 kDa protein OS=Daucus carota PE=2 SV=1[more]
PMEU1_SOLLC7.5e-1327.46Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2... [more]
PME61_ARATH2.2e-1232.47Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=P... [more]
PME3_ARATH2.8e-1229.47Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S... [more]
PME34_ARATH3.1e-1133.33Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=P... [more]
Match NameE-valueIdentityDescription
A0A0A0LWR8_CUCSA1.3e-7278.89Uncharacterized protein OS=Cucumis sativus GN=Csa_1G597770 PE=4 SV=1[more]
A0A061GSW0_THECC4.8e-5162.23Plant invertase/pectin methylesterase inhibitor superfamily protein OS=Theobroma... [more]
F6HGM6_VITVI2.0e-4965.43Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0016g00590 PE=4 SV=... [more]
A0A067KCU5_JATCU1.3e-4858.29Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07533 PE=4 SV=1[more]
B9RR57_RICCO2.9e-4859.5721 kDa protein, putative OS=Ricinus communis GN=RCOM_0710110 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G20740.12.5e-4350.79 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62770.12.0e-2738.10 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G25260.12.0e-2741.58 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G23205.12.0e-2743.16 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62760.11.7e-2638.14 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449452771|ref|XP_004144132.1|1.9e-7278.89PREDICTED: 21 kDa protein [Cucumis sativus][more]
gi|700211234|gb|KGN66330.1|1.9e-7278.89hypothetical protein Csa_1G597770 [Cucumis sativus][more]
gi|659100173|ref|XP_008450965.1|1.3e-7076.14PREDICTED: 21 kDa protein [Cucumis melo][more]
gi|590574990|ref|XP_007012560.1|6.9e-5162.23Plant invertase/pectin methylesterase inhibitor superfamily protein [Theobroma c... [more]
gi|225444639|ref|XP_002276089.1|2.9e-4965.43PREDICTED: 21 kDa protein [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g02780.1Cp4.1LG18g02780.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 27..187
score: 1.7
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 29..182
score: 2.5
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 26..183
score: 2.6
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 3..187
score: 4.9
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 28..187
score: 1.96
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 4..196
score: 2.2
NoneNo IPR availablePANTHERPTHR31080:SF26SUBFAMILY NOT NAMEDcoord: 4..196
score: 2.2