Cp4.1LG18g02690 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG18g02690
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptiontRNA (Adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
LocationCp4.1LG18 : 4276237 .. 4281543 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTGACGCGTACTCTTTAATGATAAGATATTGAGTAGATGTGTAATACGTTACGGTTGAAATACTATGACTTAAGGTGTTTGTGGGTTGGGTCGGATTAGATTGTGTGACGTCCCACATTGGTTGAGGAGGAGAACAAACCACGATTTATGGGTGTGGAAACCTTCCTCTAGCAGACGCGTTTTAAAACTTTGAGGGGAAACTCGAAGGAGAAAGTCCAAGGAGGACAATATCTACAAGCGGTGGATCTGGGTCGTTACAAATGGTATCAGAGTCAGGTTTCGGACGAAGTGCCAGTAAGGACGCTGGACCCCAAAGAGGGGTGGATTTGGGAGTGTTCCCACGTTGATTGGAGGAAGGAAAAGTGCCAGCGAGGACAATATTTACGCGGTGGATATGGGTCGTTATAGATTCCCAAATGTTTGAGTTATAAATTTTTTCAGAACTCTTATTAAATAACGAACTTAACTCAACTCTAGAATTATTTTGATCATTTATTCAAATTTTTATTATAATAAAAGTAAAATGTTAGTCGGTAAAACATTTTTTTTTATTAATTTTTACACATTTAATTAAAATTTATCATTTATTTCGGTATATATTTTAAAAAGTTCTTTTTTAAGAGTTGTTGGAGAACGCTTATGTTTTCTAAAACATTTCTCTCGAGTGTAACTCGAGGCTCGAATATACGTCGTTATTGTCCGCAAAGTGAAAATTTCAGACGGATTAACTTGTTCGCTAGATTATAACAAGTGTTGCATCATCGCTGTATGGTTGCTACAAAAGTGAAATTGAAATAGGGGTCGAGAAATAAATTTAAATAATTTTAAAATCATCAAAATTAAAATTTTAGAAATTTATTATACCAATTTAAATCCATTATTTCCTTTTTTTTTCTTTTTTTTTTTTTGTAAAAAAAAAATTAATTTTGTTGGGAAATTTACCTTTGCCTTTCTTCTTCTTCGCTTAGGTCTTCTCCGCTTTATTTTCTTCCAGTACCAGTAAACCGCCGCCGCCAGCTTCCGACTTCCTCCCCTCCGACGATTGCGTTCCATCTGTAGCAGCTAAACGCCAAACCGCTCCCCCAAACTTTCAATCTTTAGGTATTCCTTTATTTCAATGTTGGAGCACACTAGAATTTTGCAGTAATTGTTTAATTTCTCCGCCGACTGATTCCTTACCGTGTTTCGATTGGCCCTGCATCATTTTACACTTGCAACGCTCTGATACTTTCTGCATCGATTTGATATAGTTTCGAGCGGTTTCACCTTTTTCTTGAATTATATGATAGTTCTTGCAAAATCTTTATGGGATTCTTCAATGATTCTTCGAAAGAGTGTAGAGAGCGAGAGAGAACTTGAAACTGTTTCGATTTTTCAATTATATGTGATTATTATGGATCTATTTTTTATTTGGTTGCTGTTTTTCCCCTAAATTTTGACAGAATCTGGTATTCTTGATTCTTTATGAACTTTTGAAGATCAAGGATGTTGCCAACTGATGACTTGAAGAAGATATCTTTCACTCGTTCTATAAGGGATGGAGATTTAGTCATTGTTTATGAGAGACATGATGTCATGAAGGCTGTGAAAGTATGTGAAAATTCAACATTTCAGAATAGGTTTGGTATGTTTAAACATTCTGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGCTTTGTTTACTTGTTAGCCCCAACTCCTGAATTATGGACTTTGGTTCTAAGCCACAGGACACAGATTCTATACATTGCGGACATTAGTTTTGTAATTATGTTCTTAGAGATAGTTCCTGGTTGTTTGATTCTAGAATCAGGGACTGGCAGTGGATCTTTGACGACGTCTTTAGCTAGGGCTGTAGCGCCTACAGGACACGTCTATACGTTTGATTTTCATGAACAGAGAGCTGCCTCGGCGAGGTAGGAACCGGGATTTGTTGTATGTTGTCAAGTTATACCCTGCAGAGTAATTGTGTTCTATGATGATTTGCTTGTCTCCCTCTTTTTTCAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTCACTGTGGGAGTTAGAGATGTTCAAGGTCAGGGCTTCCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGCTGAAACAAGACGGCGTATTATGCTCGTTCTCTCCATGTATCGAGCAAGTTCAACGGTCGGCCGAAACTCTACGATCTAAGTTTACAGGTTGTTCTGAATGACCCATTTATATACCTTCATTCCTATAGATATGTTGATATGTATTATTCACTATTGTCGTCGTTGTTGTCGTCCCTTTTGTGTAGATATAAGAACATTTGAGGTATTGCTTCGGACTTACGAGGTTCGAGAAGGTAAAACAGAGAGCAGCCAGGTTGAAGGAGGTGGTTCTGTCGAGCTTCCACCGTGCAAGAAGCGGGTGAGATCAAAGCAAGCTAACAACACGAATGGTGATTCGAGTACGGTCATGGTTAGGCCATGTAGTGAGGCCAGAGGTCACACTGGTTATCTAACATTTGCAAGACTCAGGTGCCTCTCATAACACTAATAATAGTTCATGATTTGAAGTTTTGTGTTCTTTTTCATCACTCTGAATCTTCGATATTCATTTCAATTATTGAATGTTCTATAATTTTATCTGATCACTCTTCATCGTTTAAGCTTAAATTTCATAGATCTCTTGGATTTTTCACTTCCTAACACCCATGAGTGATTCAAGAACCCTTGTGGATGAAAAGAACTGAACAGAAAGAGGGAAAAAAGTAGACAAAACTGTTCAAAATTTGGTACATAATTTCAAAACCAAACTGTGCATAGCTCAAACATAAGGAAATATTGCCATTGGCAAACAAAAGTGCACCGATGGCAACTTATTCAATGCCAAACTTTTAACACCTCTACTTGACCCCTACTATGGACATGAGAAACTGCAACTTCTGGTTAAGTAGATTGATATCATCATCATTAGCAGAATCTTTCATCGGGGAAGGTGGCTCGACTCCGATGAAGGCATATAGAGCGTTCACTTCGTCTAAGTATGTTCCGAGGGGGGTAGCCTTGTATCCTACGATCTTCTTCATCGAAGCAATCTTTTCTCTTATCTTTACCTCTCCTGATAGTTCCTTGCAGAAAGAAAAGGGAAAACTCAGGAATTTCAAACGTTCAATCCAAACCCAAGAAAGTGAGTATGCCAAAGTTGATGGCAAGAACAGATATCAATTAAAACTCAGTACTTGTTAGTCATATTCATTAGCTCGATCGGTAAACGAAGAACGAAGGAAACAAGGCCATGGTATTCGGATTGAATCGACGTCTTTATATTATGTACTGACCTCATTGGAAGACATCCTATGATCTTTATGTGGAGAATTTGCCTCAACTTTGTGTTCAATAGTCATAGCTGATATAGACAAACCCTCATCAGATTCTATCACATCAATCATTGTTACTTTAGGAAGTTGCTCAGCTTCTTCAGACTTGGTTGCCTTGTTTGTCAATGTCGTTATTGCATTTGTTTCATCACTAGATTTCGCCGACCCGACCCCTTTAATTCCGATGACAAAGGTTAGATTTTCAAATCCTCAACCCGAAAAGAGATATCGTACATTATTTGCAAATTCGTAACTTGGAATAATAAACAAAAGAAATGAATTACCTTCAGAAGTTCTATAGTCTGCATGAGGATAAACAGCAGCAGAGTCAACTTCATTCAACAAACCTAGAACACTTTTTTCGACAGAATCACTTGGTTTCTCGTCCTCCATTGAAATCTCGAGCTCTCGAACATCATCAACACCTCCATTTTCATCAACAAAACCATTGTCTCTTTGCCCCTCATCCCTGATTCTTTCGAGTAAACCTCTAGTTTCCTTCAACTCTTCTTCCTCTTCTTCCTCATCTCCAATATCTCCATTTCCTTCAAATTCCACATTGGTCACTTCAATTGCTGCCAGACCTTTGCTTCCAATATTGGTTTCGCCCTCTTCGTCCCCCTGAATGACTACCACATCGATATCGAAATCATCCAAATTCCCACCTCTCTTAGCTGTTTCCATCATATGTTTTTCTTCATCTTCATAGATGTCTTCCTCCACAATCTCTTCGTCAGCGCGGTCGAGCTTCAAGGCCGGGGGAATCGGAAGCCAAACACTCATGATCGTCTCAGTACAAGCATAAGAGGCGAGGAAAACCCCATAGGGAATTGAGGCTGCAATTCCAAAGGCTGACATGATAACCAATGGAGGAAGAACCACAGGAACAGAGGATATGGCTAAACCAGTGAGGAGAAGCTTCTTCCCCAGACTGCAACCCTTTTGCACGGCGGAGCATATCTTTTCACTGCTCAGGCCATCACCGGAGGACAGTTGTTCTTCCATATTCCAGTCTATGCTGCGATAGCCCTCCATCCTGACAAATCAAAGAAAACTTTTATCCATAATCTTGACATCAAAGATCCTTAAAGCTAAGACAGGAAGAAAAATAAACAAAACCTGATGAACCCAGGTGGTTCATCGGCCATAATCAAACAAGCTCTTCTTCCATGGCGGATAGAGAGAGAGGGAGAGAGAATAGGCGGTTGAAGAGAATGCAAGGGAAGGCGGTGGTGGATTGTGCAGAGGAAAAGGAATGCCTGCAGAAGCCTGCCACCAAGTTAAACGTGGCGGGTTTCTACTCCACCGCTTCTCTTTAAATTTAGCACTTTTCTTCCCATTCCTTACTTCTTCTACATATCAAAAACACTTGCCTTCTAACTCACGTATTCTCTCTTGACAGATGTCTCCTGGTCTATGTCGAGAGATTATTAAAGGATAAGCCCACATAACTCCTAATATTATGCATTCGTTTGAGAAGATTCTTGAAATGATGGGTTAGTGAGCTAGGCGAGAGGTATGCTCACCTATCTAACACAAGCTCATTGCCGGTAGATGTTGTTCTCTGAGCTCTTTTTTTATAGATTTTTTCAAAGTTTTAAAAATGCGTCTACTAGGGAGAAGTTTTCACGTCCTTGTAAAAAATGCATCATCTTTCTTCTAACTGATGTGGGCTCTCACAATCCACCTCTGTTTAAGGTACATCGTCATTGCTGGCACTCGTTCTTCTCTCCAATCGATGTAGGATCTCACGTTTTCCTTTAGCTTTCTAAATCTTGTTACGTGAACTTTACTTGAGAGCTTAAACTATGATCAAGCTTAACAAAGTAAATTGTATTTTCATCATGTGATTGTTGCAAACTATTGTCC

mRNA sequence

ATGTTGTCTTCTCCGCTTTATTTTCTTCCAGTACCAGTAAACCGCCGCCGCCAGCTTCCGACTTCCTCCCCTCCGACGATTGCGTTCCATCTATCAAGGATGTTGCCAACTGATGACTTGAAGAAGATATCTTTCACTCGTTCTATAAGGGATGGAGATTTAGTCATTGTTTATGAGAGACATGATGTCATGAAGGCTGTGAAAGTATGTGAAAATTCAACATTTCAGAATAGGTTTGGTATGTTTAAACATTCTGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGCTTTGTTTACTTGTTAGCCCCAACTCCTGAATTATGGACTTTGGTTCTAAGCCACAGGACACAGATTCTATACATTGCGGACATTAGTTTTGTAATTATGTTCTTAGAGATAGTTCCTGGTTGTTTGATTCTAGAATCAGGGACTGGCAGTGGATCTTTGACGACGTCTTTAGCTAGGGCTGTAGCGCCTACAGGACACGTCTATACGTTTGATTTTCATGAACAGAGAGCTGCCTCGGCGAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTCACTGTGGGAGTTAGAGATGTTCAAGGTCAGGGCTTCCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGCTGAAACAAGACGGCGTATTATGCTCGTTCTCTCCATGTATCGAGCAAGTTCAACGGTCGGCCGAAACTCTACGATCTAAGTTTACAGATATAAGAACATTTGAGGTATTGCTTCGGACTTACGAGGTTCGAGAAGGTAAAACAGAGAGCAGCCAGGTTGAAGGAGGTGGTTCTGTCGAGCTTCCACCGTGCAAGAAGCGGGTGAGATCAAAGCAAGCTAACAACACGAATGGTGATTCGAGTACGGTCATGGTTAGGCCATGTAGTGAGGCCAGAGGTCACACTGGTTATCTAACATTTGCAAGACTCAGATGTCTCCTGGTCTATGTCGAGAGATTATTAAAGGATAAGCCCACATAACTCCTAATATTATGCATTCGTTTGAGAAGATTCTTGAAATGATGGGTTAGTGAGCTAGGCGAGAGGTATGCTCACCTATCTAACACAAGCTCATTGCCGGTAGATGTTGTTCTCTGAGCTCTTTTTTTATAGATTTTTTCAAAGTTTTAAAAATGCGTCTACTAGGGAGAAGTTTTCACGTCCTTGTAAAAAATGCATCATCTTTCTTCTAACTGATGTGGGCTCTCACAATCCACCTCTGTTTAAGGTACATCGTCATTGCTGGCACTCGTTCTTCTCTCCAATCGATGTAGGATCTCACGTTTTCCTTTAGCTTTCTAAATCTTGTTACGTGAACTTTACTTGAGAGCTTAAACTATGATCAAGCTTAACAAAGTAAATTGTATTTTCATCATGTGATTGTTGCAAACTATTGTCC

Coding sequence (CDS)

ATGTTGTCTTCTCCGCTTTATTTTCTTCCAGTACCAGTAAACCGCCGCCGCCAGCTTCCGACTTCCTCCCCTCCGACGATTGCGTTCCATCTATCAAGGATGTTGCCAACTGATGACTTGAAGAAGATATCTTTCACTCGTTCTATAAGGGATGGAGATTTAGTCATTGTTTATGAGAGACATGATGTCATGAAGGCTGTGAAAGTATGTGAAAATTCAACATTTCAGAATAGGTTTGGTATGTTTAAACATTCTGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGCTTTGTTTACTTGTTAGCCCCAACTCCTGAATTATGGACTTTGGTTCTAAGCCACAGGACACAGATTCTATACATTGCGGACATTAGTTTTGTAATTATGTTCTTAGAGATAGTTCCTGGTTGTTTGATTCTAGAATCAGGGACTGGCAGTGGATCTTTGACGACGTCTTTAGCTAGGGCTGTAGCGCCTACAGGACACGTCTATACGTTTGATTTTCATGAACAGAGAGCTGCCTCGGCGAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTCACTGTGGGAGTTAGAGATGTTCAAGGTCAGGGCTTCCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGCTGAAACAAGACGGCGTATTATGCTCGTTCTCTCCATGTATCGAGCAAGTTCAACGGTCGGCCGAAACTCTACGATCTAAGTTTACAGATATAAGAACATTTGAGGTATTGCTTCGGACTTACGAGGTTCGAGAAGGTAAAACAGAGAGCAGCCAGGTTGAAGGAGGTGGTTCTGTCGAGCTTCCACCGTGCAAGAAGCGGGTGAGATCAAAGCAAGCTAACAACACGAATGGTGATTCGAGTACGGTCATGGTTAGGCCATGTAGTGAGGCCAGAGGTCACACTGGTTATCTAACATTTGCAAGACTCAGATGTCTCCTGGTCTATGTCGAGAGATTATTAAAGGATAAGCCCACATAA

Protein sequence

MLSSPLYFLPVPVNRRRQLPTSSPPTIAFHLSRMLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFGSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLLRTYEVREGKTESSQVEGGGSVELPPCKKRVRSKQANNTNGDSSTVMVRPCSEARGHTGYLTFARLRCLLVYVERLLKDKPT
Homology
[Close]
BLAST of Cp4.1LG18g02690 vs. Swiss-Prot
Match: TRM61_MOUSE (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus musculus GN=Trmt61a PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 8.2e-63
Identity = 136/297 (45.79%), Postives = 187/297 (62.96%), Query Frame = 1 

Query: 43  ISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHS-DWIGKPFGSKVLSNKG 102
           +++   I++GD  I+   H  M AV+V   +  Q R G+ +HS D IG+PFGSKV+ ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVICSRG 63

Query: 103 GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 162
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 163 AVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLD 222
           +VAPTGH++T +FH+QRA  ARE+F++  L+  VTV  +DV   GF    V +AD+VFLD
Sbjct: 124 SVAPTGHLHTVEFHQQRADKAREEFQEHRLSQWVTVHTQDVCCSGF--GVVHVADAVFLD 183

Query: 223 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 282
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 283 EGKTESSQVEGGGSVELPPCKKRVRSKQANNTNGDSSTVMVRPCSEARGHTGYLTFA 337
                        +V LP       + + N  +  S      P  E  GHTGYLTFA
Sbjct: 244 -------------TVSLPLPDLGANNLETNMGSDASPFRSGTPMKETVGHTGYLTFA 285

[Close]
BLAST of Cp4.1LG18g02690 vs. Swiss-Prot
Match: TRM61_HUMAN (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapiens GN=TRMT61A PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.3e-62
Identity = 139/302 (46.03%), Postives = 186/302 (61.59%), Query Frame = 1 

Query: 43  ISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHS-DWIGKPFGSKVLSNKG 102
           +++   I++GD  I+   H  M AV+V   +  Q R G+ +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 103 GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 162
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 163 AVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLD 222
            +APTGH++T +FH+QRA  ARE+F++  +   VTV  +DV   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHV--ADAVFLD 183

Query: 223 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 282
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L ++ F+++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVR 243

Query: 283 EGKTESSQVEGGGSVELPPCKKRVRSKQANNTNG--DSSTVMVR---PCSEARGHTGYLT 337
                        +V LPP            T+G   S T   R   P  EA GHTGYLT
Sbjct: 244 -------------TVSLPP------PDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLT 284

[Close]
BLAST of Cp4.1LG18g02690 vs. Swiss-Prot
Match: TRM61_RAT (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus norvegicus GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 2.6e-61
Identity = 133/297 (44.78%), Postives = 185/297 (62.29%), Query Frame = 1 

Query: 43  ISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHS-DWIGKPFGSKVLSNKG 102
           +++   I++GD  I+   H  M AV+V   +  Q R G+ +HS D IG+PFGSKV  ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCSRG 63

Query: 103 GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 162
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 163 AVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLD 222
           ++APTGH++T +FH+QRA  ARE+F++  ++  VTV  +DV   GF    V +AD+VFLD
Sbjct: 124 SIAPTGHLHTVEFHQQRADKAREEFQQHRVSQWVTVHTQDVCRSGF--GVVHVADAVFLD 183

Query: 223 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 282
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 283 EGKTESSQVEGGGSVELPPCKKRVRSKQANNTNGDSSTVMVRPCSEARGHTGYLTFA 337
                        +V LP         + N  +  +      P  E  GHTGYLTFA
Sbjct: 244 -------------TVSLPLPDLGADDLEGNVGSDATPFRSGTPMKETVGHTGYLTFA 285

[Close]
BLAST of Cp4.1LG18g02690 vs. Swiss-Prot
Match: TRM61_DICDI (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyostelium discoideum GN=trmt61a PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.8e-60
Identity = 129/304 (42.43%), Postives = 190/304 (62.50%), Query Frame = 1 

Query: 49  IRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFGSKVLSNKG-GFVYLL 108
           I++GD V++Y   D M  + +  N+ + ++FG ++H + IGK +GSK+ S+ G GF +++
Sbjct: 8   IKEGDRVVMYNGKDNMAVLTMESNNVYNSKFGSYRHKNIIGKEYGSKLSSDNGNGFCHVI 67

Query: 109 APTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLARAVAPTG 168
           A TPELW++ L HRTQIL+  DIS +I  LE+  G   +ESGTGSGSL++S+AR +AP G
Sbjct: 68  AMTPELWSITLDHRTQILFNLDISTIIFNLELKNGSRAVESGTGSGSLSSSIARTIAPKG 127

Query: 169 HVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQG-QGFPEEFVGL-ADSVFLDLPQP 228
           H++TF+FHE+R   AR+DF+  GL   +TV  RD  G +GF  + +    D+VFLDLP P
Sbjct: 128 HLFTFEFHEERVKFARKDFKDNGLDQYITVTHRDACGKEGFLRQDINNDIDAVFLDLPSP 187

Query: 229 WLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLR-SKFTDIRTFEVLLRTYEVREGKTE 288
           W AI +A  ++    +LCSFSPCIEQVQ +   L  SKF +I+T EVL+RT++ R  + E
Sbjct: 188 WDAIENAIAVMHDGSMLCSFSPCIEQVQNTCLKLADSKFQEIKTIEVLIRTFDTRLQEYE 247

Query: 289 SSQV-----------EGGGSVELPPCKKRVRSKQANNTNGDSSTVMVRPCSEARGHTGYL 338
              +               ++E    + R + +           ++ +P +EARGHTGYL
Sbjct: 248 ELNLTNPYIDNNNNNNNNNNIE----ENRGKFEIGGIEGLKKDKLLSKPFTEARGHTGYL 307

[Close]
BLAST of Cp4.1LG18g02690 vs. Swiss-Prot
Match: TRM61_BOVIN (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos taurus GN=TRMT61A PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.5e-59
Identity = 134/297 (45.12%), Postives = 181/297 (60.94%), Query Frame = 1 

Query: 43  ISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHS-DWIGKPFGSKVLSNKG 102
           +++   I++GD  I+   H  M AV+V   +  Q R G+ +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 103 GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 162
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 163 AVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLD 222
            +APTGH++T +FH+QRA  ARE+F++  +   VTV  +DV   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHV--ADAVFLD 183

Query: 223 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRS-KFTDIRTFEVLLRTYEVR 282
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  F+++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNVR 243

Query: 283 EGKTESSQVEGGGSVELPPCKKRVRSKQANNTNGDSSTVMVRPCSEARGHTGYLTFA 337
                        S+ +P    R     A   +G        P  E  GHTGYLTFA
Sbjct: 244 T-----------VSLPVPDLGARPGPDAAPFRSG-------TPMKETVGHTGYLTFA 280

[Close]
BLAST of Cp4.1LG18g02690 vs. TrEMBL
Match: A0A0A0LYQ8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 1.4e-162
Identity = 294/358 (82.12%), Postives = 319/358 (89.11%), Query Frame = 1 

Query: 8   FLPVPV----------NRRRQLPTSSP----PTIAFH---------LSRMLPTDDLKKIS 67
           FLPVP           +RR+ LP++      P+  FH         L RMLPTD+LK IS
Sbjct: 347 FLPVPAAAATTRFRLPSRRKFLPSAPTHRQIPSSKFHSIEAGILDSLRRMLPTDELKSIS 406

Query: 68  FTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFGSKVLSNKGGFV 127
           FTRSIRDGDLVIVYERHD+MKAVKVC+ ST QNRFG+FKHSDWIGKPFGSK LSNKGGFV
Sbjct: 407 FTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFGSKALSNKGGFV 466

Query: 128 YLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLARAVA 187
           YLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSGSLTTSLARAVA
Sbjct: 467 YLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLARAVA 526

Query: 188 PTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLDLPQ 247
           PTGHVYTFDFHEQRA+SAREDFEKTGLTSLVTVGVRD+QGQGFPEEFVGLADSVFLDLPQ
Sbjct: 527 PTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLDLPQ 586

Query: 248 PWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLLRTYEVREGKTE 307
           PWLAIPSAERMLKQDG+LCSFSPCIEQVQRS ETLRSKFTDIRTFEVLLRTYEV+EG+TE
Sbjct: 587 PWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLLRTYEVKEGRTE 646

Query: 308 SSQVEGGGSVELPPCKKRVRSKQANNTNGDSS-TVMVRPCSEARGHTGYLTFARLRCL 342
           S+QV+GGGSV LPPCK+R+RSK++++ + +SS TVMVRPCSEARGHTGYLTFARLRCL
Sbjct: 647 SNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGYLTFARLRCL 704

[Close]
BLAST of Cp4.1LG18g02690 vs. TrEMBL
Match: F6HUY1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0066g00990 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 2.6e-140
Identity = 254/315 (80.63%), Postives = 276/315 (87.62%), Query Frame = 1 

Query: 29  FHLSRMLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWI 88
           + L RMLP D  K+ISF R I DGDLVIVYERHD MKAVKVCE ST QNRFG+FKHSDWI
Sbjct: 21  YKLLRMLPIDAEKRISFKRCISDGDLVIVYERHDNMKAVKVCEGSTLQNRFGVFKHSDWI 80

Query: 89  GKPFGSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILES 148
           GKPFGSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADIS VIM+LEI+PGCL+LES
Sbjct: 81  GKPFGSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISLVIMYLEIIPGCLVLES 140

Query: 149 GTGSGSLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFP 208
           GTGSGSLTTSLARAVAP GHVYTFDFHEQRAASAREDFEK GL+S VTVGVRD+QG+GFP
Sbjct: 141 GTGSGSLTTSLARAVAPNGHVYTFDFHEQRAASAREDFEKIGLSSFVTVGVRDIQGEGFP 200

Query: 209 EEFVGLADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRT 268
           +EF GLADSVFLDLPQPWLAIPSA +MLK+DGVLCSFSPCIEQVQRS+ETL S FTDIRT
Sbjct: 201 DEFSGLADSVFLDLPQPWLAIPSAGKMLKEDGVLCSFSPCIEQVQRSSETLTSNFTDIRT 260

Query: 269 FEVLLRTYEVREGKTESSQVEGGGSVELPPCKKRVRSKQANNTN--GDSSTVMVRPCSEA 328
           FEVLLRTYEVREGK + SQ   GG +   P K+R RS +A+N      S+TVM RP +EA
Sbjct: 261 FEVLLRTYEVREGKMDCSQGNEGGFLGPRPGKRRQRSGEASNEGEIPTSTTVMARPAAEA 320

Query: 329 RGHTGYLTFARLRCL 342
           RGHTG+LTFARL+CL
Sbjct: 321 RGHTGFLTFARLKCL 335

[Close]
BLAST of Cp4.1LG18g02690 vs. TrEMBL
Match: B9S623_RICCO (tRNA, putative OS=Ricinus communis GN=RCOM_1063210 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 3.4e-140
Identity = 247/310 (79.68%), Postives = 273/310 (88.06%), Query Frame = 1 

Query: 34  MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 93
           ML TD  +++SFTR I DGDLVIVYE+HD MKA+KVCE S  QNRFG+FKHSDWIGKPFG
Sbjct: 1   MLHTDPTRRLSFTRCITDGDLVIVYEKHDTMKAIKVCETSVLQNRFGVFKHSDWIGKPFG 60

Query: 94  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 153
           SKV SNKGGFVYLLAPTPELWTLVLSHRTQILYIADISF+I +LEIVPGCL+LESGTGSG
Sbjct: 61  SKVFSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFLITYLEIVPGCLVLESGTGSG 120

Query: 154 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 213
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTG++SLVTVGVRD+QG+GFP+E+ G
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGVSSLVTVGVRDIQGEGFPDEYSG 180

Query: 214 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 273
           L DSVFLDLPQPWLAIPSA +MLKQDGVLCSFSPCIEQVQRS ETLRS FTDIRTFEVLL
Sbjct: 181 LVDSVFLDLPQPWLAIPSAAKMLKQDGVLCSFSPCIEQVQRSCETLRSNFTDIRTFEVLL 240

Query: 274 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKQANNTNGDSST--VMVRPCSEARGHTG 333
           RTYEVRE K +  Q E G S+  PP K+R RS + +N    SS+  +M RPC ++RGHTG
Sbjct: 241 RTYEVREVKMDCGQGEEGISLGSPPYKRRQRSSEGSNVQNSSSSPVIMARPCGDSRGHTG 300

Query: 334 YLTFARLRCL 342
           YLTFARL+CL
Sbjct: 301 YLTFARLKCL 310

[Close]
BLAST of Cp4.1LG18g02690 vs. TrEMBL
Match: A0A061EBT4_THECC (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein OS=Theobroma cacao GN=TCM_016926 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 7.5e-140
Identity = 248/310 (80.00%), Postives = 275/310 (88.71%), Query Frame = 1 

Query: 34  MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 93
           ML TD  KKISF R IRDGDLVIVYERHD MKAVKVC+NS  QNRFG+FKHSDWIGKPFG
Sbjct: 1   MLLTDPSKKISFNRCIRDGDLVIVYERHDSMKAVKVCKNSVLQNRFGVFKHSDWIGKPFG 60

Query: 94  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 153
           SKV SNK GFVYLL PTPELWTLVLSHRTQILYIADIS VIM+LEIVPGCL+LESGTGSG
Sbjct: 61  SKVFSNKNGFVYLLTPTPELWTLVLSHRTQILYIADISLVIMYLEIVPGCLVLESGTGSG 120

Query: 154 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 213
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTG+++LVTVGVRD+QG+GFP+EF G
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGISTLVTVGVRDIQGEGFPDEFSG 180

Query: 214 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 273
           LADSVFLDLPQPWLAIPSA +MLKQDG+LCSFSPCIEQVQRS ETLRS FTDIRTFE+LL
Sbjct: 181 LADSVFLDLPQPWLAIPSAGKMLKQDGILCSFSPCIEQVQRSCETLRSNFTDIRTFEILL 240

Query: 274 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKQANNTNGDSS-TVMVRPCSEARGHTGY 333
           R+Y+VRE + + S+V+ G S+  PPCK+R  S + +  +  SS  VM RP +EARGHTGY
Sbjct: 241 RSYDVREWRMDCSKVDEGTSIACPPCKRRQPSGEGSVRDHQSSPAVMARPSAEARGHTGY 300

Query: 334 LTFARLRCLL 343
           LTFARL+C+L
Sbjct: 301 LTFARLKCIL 310

[Close]
BLAST of Cp4.1LG18g02690 vs. TrEMBL
Match: A0A165ZPE5_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_009028 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 1.7e-139
Identity = 246/314 (78.34%), Postives = 274/314 (87.26%), Query Frame = 1 

Query: 29  FHLSRMLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWI 88
           F + +MLPTD +K++SF R + +GDLVIVYERHD MKA+KVCE++  QNRFG+FKHSDWI
Sbjct: 74  FVIRKMLPTDSVKRLSFKRCVSEGDLVIVYERHDNMKAIKVCESAVLQNRFGVFKHSDWI 133

Query: 89  GKPFGSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILES 148
           GKPFGSKV S+KGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIM+LEIVPGC++LES
Sbjct: 134 GKPFGSKVFSSKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMYLEIVPGCVVLES 193

Query: 149 GTGSGSLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFP 208
           GTGSGSL+TSLARAVAP+GHVYTFDFHEQRAASAREDFEKTGL+SLVTVGVRD+QG+GFP
Sbjct: 194 GTGSGSLSTSLARAVAPSGHVYTFDFHEQRAASAREDFEKTGLSSLVTVGVRDIQGEGFP 253

Query: 209 EEFVGLADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRT 268
           E+F G ADSVFLDLPQPWLAIPS  +MLKQDG+LCSFSPCIEQVQRS ETLRS FTDIRT
Sbjct: 254 EKFTGKADSVFLDLPQPWLAIPSVGKMLKQDGILCSFSPCIEQVQRSCETLRSNFTDIRT 313

Query: 269 FEVLLRTYEVREGKTESSQVEGGGSVELPPCKKRVRSKQANN--TNGDSSTVMVRPCSEA 328
           FEVLLRTYEVRE K  S Q +   S ELPPCK++ R  +  N   N     VM RP SEA
Sbjct: 314 FEVLLRTYEVREVKMNSFQGDEDRSTELPPCKRKQRLNEEGNGLENPSPPVVMARPSSEA 373

Query: 329 RGHTGYLTFARLRC 341
           RGHTGYLTFARL+C
Sbjct: 374 RGHTGYLTFARLKC 387

[Close]
BLAST of Cp4.1LG18g02690 vs. TAIR10
Match: AT5G14600.1 (AT5G14600.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 474.9 bits (1221), Expect = 4.2e-134
Identity = 234/317 (73.82%), Postives = 268/317 (84.54%), Query Frame = 1 

Query: 34  MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 93
           MLPT+  K  SF R I DGDLVIVYERHDVMK VKV ++   QNRFG++KHSDWIGKP G
Sbjct: 1   MLPTESKKAFSFKRCIEDGDLVIVYERHDVMKPVKVSKDGVLQNRFGVYKHSDWIGKPLG 60

Query: 94  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 153
           +KV SNKG FVYLLAPTPELWTLVLSHRTQILYIADISFV+M+LE+VPGC++LESGTGSG
Sbjct: 61  TKVFSNKGKFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEVVPGCVVLESGTGSG 120

Query: 154 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 213
           SL+TSLARAVAPTGHVY+FDFHEQRA SAREDFEKTG++SLVTV VRD+QG+GFPE+  G
Sbjct: 121 SLSTSLARAVAPTGHVYSFDFHEQRAVSAREDFEKTGISSLVTVEVRDIQGEGFPEKLSG 180

Query: 214 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 273
           LADSVFLDLPQPWLA+PSA +MLKQDGVLCSFSPCIEQVQR+ E LRS F +IRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAVPSAAKMLKQDGVLCSFSPCIEQVQRTCEVLRSDFIEIRTFEVLL 240

Query: 274 RTYEVREGKTESSQVEGGGSVE----LPPCKKRVRSKQ-----ANNTNGDSSTVMVRPCS 333
           RTYEV+E K ++S + G    E    + PCK++ RS +      +N++  SS VM RPCS
Sbjct: 241 RTYEVKEVKMDTSSMVGESHEEDNEGMRPCKRKHRSNENDTVSQDNSSNASSVVMARPCS 300

Query: 334 EARGHTGYLTFARLRCL 342
           EARGHTGYLTFARL+CL
Sbjct: 301 EARGHTGYLTFARLQCL 317

[Close]
BLAST of Cp4.1LG18g02690 vs. NCBI nr
Match: gi|659118407|ref|XP_008459104.1| (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1 [Cucumis melo])

HSP 1 Score: 581.3 bits (1497), Expect = 1.2e-162
Identity = 293/354 (82.77%), Postives = 315/354 (88.98%), Query Frame = 1 

Query: 2   LSSPLYFLPVPVNRRRQLPTSSPPTIA-------------FHLSRMLPTDDLKKISFTRS 61
           LSS L F       R Q+P S  P I+               LSRMLPTD+LK + FTRS
Sbjct: 13  LSSALLFFQYQPPPRHQIPPSFSPAISSFPSNSTPISLLQIPLSRMLPTDELKNLYFTRS 72

Query: 62  IRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFGSKVLSNKGGFVYLLA 121
           IRDGDLVIVYERHD+MKAVKVCE ST QNRFG+FKHSDWIGKPFGSK LSNKGGFVYLLA
Sbjct: 73  IRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFGSKALSNKGGFVYLLA 132

Query: 122 PTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLARAVAPTGH 181
           PTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSGSLTTSLARAVAPTGH
Sbjct: 133 PTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLARAVAPTGH 192

Query: 182 VYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLDLPQPWLA 241
           VYTFDFHEQRA+SAREDFEKTGLTSLVTVGVRD+QGQGFPEEFVGLADSVFLDLPQPWLA
Sbjct: 193 VYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLDLPQPWLA 252

Query: 242 IPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLLRTYEVREGKTESSQV 301
           IPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLLRTYEV+EG+TES++V
Sbjct: 253 IPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLLRTYEVKEGRTESNEV 312

Query: 302 EGGGSVELPPCKKRVRSKQANNTNGDSS-TVMVRPCSEARGHTGYLTFARLRCL 342
           +GGGSV LPPCK+R+RSK++++ + +SS TVMVRPCSEARGHTGYLTFARL CL
Sbjct: 313 DGGGSVALPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGYLTFARLGCL 366

[Close]
BLAST of Cp4.1LG18g02690 vs. NCBI nr
Match: gi|700210876|gb|KGN65972.1| (hypothetical protein Csa_1G555080 [Cucumis sativus])

HSP 1 Score: 580.5 bits (1495), Expect = 2.0e-162
Identity = 294/358 (82.12%), Postives = 319/358 (89.11%), Query Frame = 1 

Query: 8   FLPVPV----------NRRRQLPTSSP----PTIAFH---------LSRMLPTDDLKKIS 67
           FLPVP           +RR+ LP++      P+  FH         L RMLPTD+LK IS
Sbjct: 347 FLPVPAAAATTRFRLPSRRKFLPSAPTHRQIPSSKFHSIEAGILDSLRRMLPTDELKSIS 406

Query: 68  FTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFGSKVLSNKGGFV 127
           FTRSIRDGDLVIVYERHD+MKAVKVC+ ST QNRFG+FKHSDWIGKPFGSK LSNKGGFV
Sbjct: 407 FTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFGSKALSNKGGFV 466

Query: 128 YLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLARAVA 187
           YLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSGSLTTSLARAVA
Sbjct: 467 YLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLARAVA 526

Query: 188 PTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLDLPQ 247
           PTGHVYTFDFHEQRA+SAREDFEKTGLTSLVTVGVRD+QGQGFPEEFVGLADSVFLDLPQ
Sbjct: 527 PTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLDLPQ 586

Query: 248 PWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLLRTYEVREGKTE 307
           PWLAIPSAERMLKQDG+LCSFSPCIEQVQRS ETLRSKFTDIRTFEVLLRTYEV+EG+TE
Sbjct: 587 PWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLLRTYEVKEGRTE 646

Query: 308 SSQVEGGGSVELPPCKKRVRSKQANNTNGDSS-TVMVRPCSEARGHTGYLTFARLRCL 342
           S+QV+GGGSV LPPCK+R+RSK++++ + +SS TVMVRPCSEARGHTGYLTFARLRCL
Sbjct: 647 SNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGYLTFARLRCL 704

[Close]
BLAST of Cp4.1LG18g02690 vs. NCBI nr
Match: gi|449435564|ref|XP_004135565.1| (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Cucumis sativus])

HSP 1 Score: 573.5 bits (1477), Expect = 2.5e-160
Identity = 280/309 (90.61%), Postives = 300/309 (97.09%), Query Frame = 1 

Query: 34  MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 93
           MLPTD+LK ISFTRSIRDGDLVIVYERHD+MKAVKVC+ ST QNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFG 60

Query: 94  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 153
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120

Query: 154 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 213
           SLTTSLARAVAPTGHVYTFDFHEQRA+SAREDFEKTGLTSLVTVGVRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180

Query: 214 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 273
           LADSVFLDLPQPWLAIPSAERMLKQDG+LCSFSPCIEQVQRS ETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLL 240

Query: 274 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKQANNTNGDSS-TVMVRPCSEARGHTGY 333
           RTYEV+EG+TES+QV+GGGSV LPPCK+R+RSK++++ + +SS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 300

Query: 334 LTFARLRCL 342
           LTFARLRCL
Sbjct: 301 LTFARLRCL 309

[Close]
BLAST of Cp4.1LG18g02690 vs. NCBI nr
Match: gi|659118409|ref|XP_008459105.1| (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X2 [Cucumis melo])

HSP 1 Score: 570.9 bits (1470), Expect = 1.6e-159
Identity = 279/309 (90.29%), Postives = 299/309 (96.76%), Query Frame = 1 

Query: 34  MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 93
           MLPTD+LK + FTRSIRDGDLVIVYERHD+MKAVKVCE ST QNRFG+FKHSDWIGKPFG
Sbjct: 7   MLPTDELKNLYFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 66

Query: 94  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 153
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 67  SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 126

Query: 154 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 213
           SLTTSLARAVAPTGHVYTFDFHEQRA+SAREDFEKTGLTSLVTVGVRD+QGQGFPEEFVG
Sbjct: 127 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 186

Query: 214 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 273
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 187 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 246

Query: 274 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKQANNTNGDSS-TVMVRPCSEARGHTGY 333
           RTYEV+EG+TES++V+GGGSV LPPCK+R+RSK++++ + +SS TVMVRPCSEARGHTGY
Sbjct: 247 RTYEVKEGRTESNEVDGGGSVALPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 306

Query: 334 LTFARLRCL 342
           LTFARL CL
Sbjct: 307 LTFARLGCL 315

[Close]
BLAST of Cp4.1LG18g02690 vs. NCBI nr
Match: gi|255560858|ref|XP_002521442.1| (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Ricinus communis])

HSP 1 Score: 506.1 bits (1302), Expect = 4.8e-140
Identity = 247/310 (79.68%), Postives = 273/310 (88.06%), Query Frame = 1 

Query: 34  MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 93
           ML TD  +++SFTR I DGDLVIVYE+HD MKA+KVCE S  QNRFG+FKHSDWIGKPFG
Sbjct: 1   MLHTDPTRRLSFTRCITDGDLVIVYEKHDTMKAIKVCETSVLQNRFGVFKHSDWIGKPFG 60

Query: 94  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 153
           SKV SNKGGFVYLLAPTPELWTLVLSHRTQILYIADISF+I +LEIVPGCL+LESGTGSG
Sbjct: 61  SKVFSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFLITYLEIVPGCLVLESGTGSG 120

Query: 154 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 213
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTG++SLVTVGVRD+QG+GFP+E+ G
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGVSSLVTVGVRDIQGEGFPDEYSG 180

Query: 214 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 273
           L DSVFLDLPQPWLAIPSA +MLKQDGVLCSFSPCIEQVQRS ETLRS FTDIRTFEVLL
Sbjct: 181 LVDSVFLDLPQPWLAIPSAAKMLKQDGVLCSFSPCIEQVQRSCETLRSNFTDIRTFEVLL 240

Query: 274 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKQANNTNGDSST--VMVRPCSEARGHTG 333
           RTYEVRE K +  Q E G S+  PP K+R RS + +N    SS+  +M RPC ++RGHTG
Sbjct: 241 RTYEVREVKMDCGQGEEGISLGSPPYKRRQRSSEGSNVQNSSSSPVIMARPCGDSRGHTG 300

Query: 334 YLTFARLRCL 342
           YLTFARL+CL
Sbjct: 301 YLTFARLKCL 310

The following BLAST results are available for this feature:
BLAST of Cp4.1LG18g02690 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TRM61_MOUSE8.2e-6345.79tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus muscu... [more]
TRM61_HUMAN5.3e-6246.03tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapi... [more]
TRM61_RAT2.6e-6144.78tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus no... [more]
TRM61_DICDI3.8e-6042.43tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyoste... [more]
TRM61_BOVIN2.5e-5945.12tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos tauru... [more]
BLAST of Cp4.1LG18g02690 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LYQ8_CUCSA1.4e-16282.12Uncharacterized protein OS=Cucumis sativus GN=Csa_1G555080 PE=3 SV=1[more]
F6HUY1_VITVI2.6e-14080.63Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0066g00990 PE=4 SV=... [more]
B9S623_RICCO3.4e-14079.68tRNA, putative OS=Ricinus communis GN=RCOM_1063210 PE=4 SV=1[more]
A0A061EBT4_THECC7.5e-14080.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein OS=Theo... [more]
A0A165ZPE5_DAUCA1.7e-13978.34Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_009028 PE=4 SV=1[more]
BLAST of Cp4.1LG18g02690 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G14600.14.2e-13473.82 S-adenosyl-L-methionine-dependent methyltransferases superfamily pro... [more]
BLAST of Cp4.1LG18g02690 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659118407|ref|XP_008459104.1|1.2e-16282.77PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A i... [more]
gi|700210876|gb|KGN65972.1|2.0e-16282.12hypothetical protein Csa_1G555080 [Cucumis sativus][more]
gi|449435564|ref|XP_004135565.1|2.5e-16090.61PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [... [more]
gi|659118409|ref|XP_008459105.1|1.6e-15990.29PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A i... [more]
gi|255560858|ref|XP_002521442.1|4.8e-14079.68PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0031515tRNA (m1A) methyltransferase complex
Vocabulary: Biological Process
TermDefinition
GO:0030488tRNA methylation
Vocabulary: Molecular Function
TermDefinition
GO:0016429tRNA (adenine-N1-)-methyltransferase activity
Vocabulary: INTERPRO
TermDefinition
IPR014816tRNA_MeTrfase_Gcd14
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0030488 tRNA methylation
cellular_component GO:0031515 tRNA (m1A) methyltransferase complex
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016429 tRNA (adenine-N1-)-methyltransferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g02690.1Cp4.1LG18g02690.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PIRPIRSF017269GCD14coord: 43..341
score: 1.0
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PFAMPF08704GCD14coord: 102..337
score: 5.1
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PROFILEPS51620SAM_TRM61coord: 52..339
score: 98
NoneNo IPR availableGENE3DG3DSA:3.10.330.20coord: 48..108
score: 1.1
NoneNo IPR availablePANTHERPTHR12133UNCHARACTERIZED METHYLTRANSFERASEcoord: 31..345
score: 1.5E
NoneNo IPR availablePANTHERPTHR12133:SF2TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61Acoord: 31..345
score: 1.5E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG18g02690CmoCh10G008460Cucurbita moschata (Rifu)cmocpeB055
Cp4.1LG18g02690Carg12910Silver-seed gourdcarcpeB0138
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG18g02690Silver-seed gourdcarcpeB1341
Cp4.1LG18g02690Cucumber (Chinese Long) v3cpecucB0428
Cp4.1LG18g02690Cucurbita maxima (Rimu)cmacpeB080
Cp4.1LG18g02690Wild cucumber (PI 183967)cpecpiB350
Cp4.1LG18g02690Cucumber (Chinese Long) v2cpecuB349
Cp4.1LG18g02690Bottle gourd (USVL1VR-Ls)cpelsiB299
Cp4.1LG18g02690Watermelon (Charleston Gray)cpewcgB322
Cp4.1LG18g02690Watermelon (97103) v1cpewmB350
Cp4.1LG18g02690Cucumber (Gy14) v2cgybcpeB039