Cp4.1LG16g08450 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG16g08450
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionHydroxymethylglutaryl-CoA lyase
LocationCp4.1LG16 : 8101294 .. 8107644 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGATAAAAAATTAATTTGAAGAGAGGAAGCTTGAGAGAATGAAGAATTGACCAACTTCCAACTGACAACAACCTACCACCTCTCCTTCCCCAGAACAGAAGAAACCACAATTTATTGATACTGACCTAAATTCTGCGATGGAAAAGAGAGAGAAAATATATGCAAATTTCCGCATTTACGACGTCCACCAACCCAACAAACACAGGCAAATTTCAATTTCATTTTTGTTCGCAACGCGGTTTGTTGCTCTTCAATTTCATCGCATCGGTTACCATTTCATCATCTCTACCGCGCTGAAGCGTTCCTTTCCCGATTTCCCGCTTTAGGTTCGATTCTTTTCATCTCTTCTCTTTCCTTTTTGCCTTTTTTTGTTTAGGTTTTTTGGAATTTTTTTCTGGAATTTCTGTTGCTCGTGTTTGGCTGTCCAAAAAGTGTAGGTATTATAGAAGTCATTGATATTGTTACCATTCGCTGTTTCTATGGTGTTCATAAATTATACAATCTGTACGCTAATTGGTTCGTGTGAAGGAGTTGGCTCGATTTTGTAATTTAGTTTTTCTCTTTTGAAAAAATGGGTTATGATCATCGCCTGCGAGTTTTCTCTAATCCTGAATTGGGATCGCTTCTATTAGTTTTTTTTGAACATGCTTGTTACGGAAGATTGTTGAAATTCAGTTGTTATATTGGATATAGTTCTCCTATTACTTACTTTGAGCCCTCAGTTTTCTATCATATGCCATCTCCTTGATGCTATCCTGAATTTTACAAACAATGTTAGAACTTGTGGATGGATTGTTGAAACCTCCCCTTTAATCTTTTGATTTTTAGGGGGCCAATGAAATTTAACTTTCCTTATATGGGGAAGTTGTGAGATGTCCTTGTAATGGTGTATTTCAAGAAAACATGTAACAACTCAAGCCCATTGGTAGTAGATATTGTCCTCTTTGGGCTTTTCCTTTCGGGCTTACCCTCAAGGTTTTTAAAATACGTCTGCTAGGAAGAAGTTTCCACACCCTTATAAAGAATGCTTCGTTCTCCTCCCCAACCGATGTGGGATCCATCCCCTTCGGGGCCCAGCGTCCTCACTGGCACTCTTTCCCTTTTCCAATCCATGTGGGACCCCCCAATTCACCCCTCTTCGCGGCCCAACGTCCTTGCTGGCACACCGTCTCGTGTCCACCCCCTTTCGGGACTCAGCCTCCTCACTGGCACATCGCCCGGTGTCTGGCGCTGATACCAATTGTAACGGCCTAAGCCCATCGTTAGCTGATATTGTCTTCTTTAGACTTTCCCTTTTGAGTTTCCCCTCAAAGTTTTTAAAACGCGTCTACTAGGGAGAGGTTTCCACACCCTTATAAAAAATGCTTCGTTCTCCTCCCCAACCAATGTGGGATCTCACACTTACATTGGAAGATGGAATCTATATATACCATATGGTGGACATGTATGCATTGGAGATTTTTTTATGGCAAGAAGAATATTTCTTTATCAATTACACACAAGATCAGAATGAGCATGCCAGTGTGCTCTTGCCATTCCATTCTTGAGCTCAGTGTACAAAACATCTAGGGAATAGTTGCTTGTCTTGATTGGTACATTTGTGAATGAGAGGTGTTTGTTCAGTGCAACCTTGAGATTGAGTATTGGAGGATTGTGCATTTAAGAATTTAATATTGACGTGCAATGGAAGAATTATGAAAGAGGAATTTCTGGACTTCTATTAGAAAAATAAAGCTCATTACAGAATTTGTAGCTTCTCATTATCTGTACTATTTAATGTGGTTATGGTGTATTTAAACGGTGTAGATACAAAATTACTAGAAGTTGTTTTAGCTATGACGAGTAAGATTAATGAATTTTTTGGATAACTGGGAGTTTGAGCTTTGCTCACCTTGAGGTACCTGTGACCGCCCTAGAGCCTGGACTTGAAGATATCAAGGTAATAGCCCAAGTCCACCGCTTGCAGATATTGCCCGCTTTAGATTTTCCCTTTTGGGCTTCCCCTCAAAGTTTTTAGATCGCATCTGTTAGGGAGAGGTTTCCAACCCTTATAAAGAATGCTTCATTCTCCTCCTCAACCGATGTGAAATCTCACATCAAAGTTTTTGGTATTAAGCTCACCCGAAGGTTTTAACTTGGTACCTCTAAGCTGGTATGACTAAGAAACCCCAAAAGCCCTTGTCATCAATTTGCAGACGATTTAGTACAAATGTGCATTTTATCTTATGCTGGAGCCCCTGCCCTGCGGCTTGGGAGAGCTATTTGCATCTAAATTCATCAATTGGAAGTTGAGACTTTCTTTCTTAATTGGCATGTGGTGAATGCACGAATAGGTCATCCATTGTATGAAATTTGATACCTATGAAATTTCATTATATATTGATCTTCTTTACTTATATATGAGAGACTAAAAGAATACATTTAATAATTGCTTGCTACTTCAATCAAACAATATCACACCAGTTGATAGAAGCAATTATAAAAATAGATTTAACTAGTCTATTATTAAGGTTTCATCTGTTATTTGTTCTTCTGCAAGTAAGATAGCTATATCACTCTGGACGTGGCAGAAAATTTATTTGGTTTATTTCTCAAACTCAGTTCTGGTTTTTGCTTCTGCCTCCATAATGTCGAGTTTGGAGGAGCCACTTGGTCTTGACAAGTTGCCAAGCATGAGCACAATTGACCGGCTACAAAGATTTTCATCTGGTGCCTGCCGACCAATAGGAGATGACATGGGAATGGGTATCTGCTGGATTCAAGGGGGGAGAAGCAGCTCATCCAATACTTGCAAGTAAGCAGAATATTAAAGTCTTTTGGAAGAAAACCAAACTTTTCTTAATTTTTTCAGCTTGCTTTTGCACATTTTGGTTCTGCAATTCTTGTTGGTATGGGATGGTTCTATGACAGAAGACCCACCTTGCAAACAATCTGGCCGCATTGTTCTTTCAGTTATTTCTTACTAAATTGTCATTTTTGCAATCAACTCGGCCACACTTTTCTCCAATATGACATATCATCCTCAGGGAATATGATGAGGATGCTAGAGAGACGTTTCCGTGGAGAAGACATAGGAAAGACACATCCCAAAATGGCTCTGTCAATAGGAGGACGATGAGCGCAGGCCCGGAAAAATTAGTATCTGGAAATTTTTGTGATTCACATTGTTCTCCAGGTCATGAATACAAGACTAAGAGTAGTAGCAACAACATACAGCATACTACAAATAAGGTGATTTGATACTTATTAGACCTGTATACCTGTAACCACTCTCTTTGTTTTACCCGCATCCATGCATTTTTATATCCTTTTAGTTGTTTTTCTTGCTATACCTCGTACCTACCAAATTCTAGATGTTTTGCAAACTTTTATCAGATGATTTGGTGGGCTATGGAATCTCTCTCTCTTAAAATGTGACCTTTTCATACTTATTTCGGACACGGGCTTGCAGTTTTTGAAAGATATACCAAAGTTTGTGAAGATAGTAGAAGTTGGTCCAAGGGATGGGCTTCAAAATGAGAAGGGTATGGTACCTACATCTGTGAAGGTGGAATTGATCCATAGACTAGTATCTTCAGGATTGCCTGTGGTTGAGGCTACCAGTTTTGTATCACCTACACGGGTGCCTCAGGTACTTTTGGTTTAAGTTCCTATCTTTTTTAGCCATAGTTTTAAAATGATATTAAAAATAGTCGTTTGAATCTCTTTTGAGTTCATTAAGGTATATACTCAGTCTTCTTTCTACTTTAGTGGTCTTGCCTTTACTGTTTACATCGCTTGATTGCTACAAGACGATCTAATGTTGCTGTGGATCAAAGTTCCTTAAAACTTCTCATCATTTAGGATTTCTATGTTCTGATATCAGCTGATAAGGAAACAGAGTGGGAGTTGAAGCTTTTCTCTCAATAGTTTGATCAAATGAAAACATAGTTCATTTCTTAGATTTACCATACTTTATATCACTAGTGCTCCAATATGTGAGTTACGTAAAGTATTTTTTTCTTTTTTATCCTTTGTCCAGTTATCTGATGCAGCTGAAGTCATGGAAGCCGTACGTAATTTGGAAGGTGCTAGGTTGCCAGTTTTAACTCCTAATTTGAAAGTGGGTCATCTTGTAAAATAATGTTGATTTTTTTTTTTTTTCAAACATGGATGGATTGAATCCTCGCTTATAAAACTTCACCTTCTTCCAGGGGTTTCGAGCAGCTGTTGCGGCTGGAGCAAAGGAAGTGGCAGTCTTTGCATCAGCCTCTGAATTATTTTCAAAATCTAACATCAATTGTAGCATTGAAGAAAGTCTTGATCGTTACCGAGCGGTAACTTCTGCTGCAAAAGAGCTTGCAATCCCTGTTCGAGGGTATGCTTCCATGACTCAGTGTCCATATAAATGGCTATTGGAATTTGGTTTTAATTTCCTCTGAATTTTGGTTTTAAGTTTTGAACACGACTTGTTTTCTAGAATGGTTCTCCTCATTCTGTTCTTTTGAAGGGTTCTCTTATTGCTATAACTGGCTATGGATTTGTATAGGTATGTATCATGTGTTGTTGGTTGTCCAACAGAAGGATCAATTCCTCCATCTAAAGTCGCTTATGTAGCGAAGGAGCTTTATGAGATGGGTTGCTTTGAAATTTCTCTTGGAGACACGATCGGAGTTGCCACGCCCGGTAACAATTTAAACCTTTCTTTCTCAAAATCGAAATATAGATTGCTTTCATCTTCTTCGCTCTCTTATAGTATCCTCTGTTGGCACAGGGACTGTTGTTCCCATGCTTGAAGCTGTGATGGCTGTCGTTCCGGTGGACAAGCTTGCAGTCCACTTCCATGACACTTATGGACAATCACTTCCAAATATTCTAGTATCCCTCCAAGTAAGGAATCTTTTGAAGCTGAAGTTTGAGCCCGTTTGGAATGACTTTCTAAGTGTTCAAAATACTTCTCAAATAGATCCTCCTACATCTATATATAAACATTGGTTTATCAAGAATATGCAAGAAAAACACTTCGGATTGTTGATTCATCGCCAGAAATGACTTAGAAACAATAGTTCATTATCTAATGGATTTTTTCGTTCTAATACTCTATCCAAGAGTTATCGGTATTTATCAAATTTGTTCCTATTTAACAGAACATTATGATAATGACAGGGTTGAGAAAAATACACTTGAAAGTCATTTCAAACATATACTTGGTCCCTTCTAAACTTGCTTTCCAGATATATGGATGCAAGTTGCAAGCTTTGCCTAGGATTTCATTTCATTTGGGCCAACTTCAACCATTAACTGGTGTGTTGCTTTTATGAACAGATGGGGATTAGCACAATAGATTCCTCTGTTGCAGGTCTGGGTGGATGTCCATATGCAAAAGGAGCTTCCGGGAATGTTGCAACTGAGGATGTGGTGTACATGCTCCACGGACTTGGCATAAAAACCAATGTGGATTTGGCAAAGGTCATGTCTGCTGGGAACTTCATCAGCAACCATTTGGGGCGCCCATCGGGTTCGAAGACTTCCATTGCCTTCAACCAACTTGCAGCCGATGCTTCCAAGATGTGACACATCTTATTACTCATACATTTACACCTTACATTGAACGAATAATGAAGGGTTTCACTTCAGGAAGCTTTCTTATAGTTTCTATACTATTTTATCTGGCTGAATATGATGCAGCCCATATCTCAAACAACATAACTATTGTCTGTCATGAAGTATCAAAATTTATGGATGCGAGTCCTCCCATGATTTGGAGAGGTTATTATTTATCCCTTTTGTGTCGGTGGTCGGAATGGTTGAGATATTATCATGTTATGGAAAACTTTCTTGAAATTCACCTATTGGCGTTGGAATTCTCGACTCCACCTATTTCATTTATCAATGAAATTATTTCTCTTATCGAAAAAGATATTGTCACATCATATTGTATCGAGTAAAAATGCGTATAAGTATTAAACTACGGTCAAGCCAGAGTAAAAATGTATGTGAAAGAGAGTGTTTGCAGATTCCTTGAAAACAAGATTTTATCTGTTCCAAATCCTTGTTAGTAATTGAATCAGACTTGCACAATGTGGTCTATGGAGAGAAGATAATATATGCAATACAAGACTTATTAAGCAGAAAACCATACTATTCTGTGGAACTGCAACTGAACGACTCCAGAAAGCTCCGGGCAGCATCTGAACTCAGTTCTTCGTTAACCTGTAGTCATATTGATTATAGGATTTTCATTCTAATGTCAATGGAGGATGAAAAACTAAATATCTAATTGCACGACATGTTCTGAATGACTTACAAGTTTATTGTGAATCTGTTGAAATGAGTTACAGAATCTCTCTGCTTCAAGCTTGCCCACCTGCG

mRNA sequence

AGATAAAAAATTAATTTGAAGAGAGGAAGCTTGAGAGAATGAAGAATTGACCAACTTCCAACTGACAACAACCTACCACCTCTCCTTCCCCAGAACAGAAGAAACCACAATTTATTGATACTGACCTAAATTCTGCGATGGAAAAGAGAGAGAAAATATATGCAAATTTCCGCATTTACGACGTCCACCAACCCAACAAACACAGGCAAATTTCAATTTCATTTTTGTTCGCAACGCGGTTTGTTGCTCTTCAATTTCATCGCATCGGTTACCATTTCATCATCTCTACCGCGCTGAAGCGTTCCTTTCCCGATTTCCCGCTTTAGTTCTGGTTTTTGCTTCTGCCTCCATAATGTCGAGTTTGGAGGAGCCACTTGGTCTTGACAAGTTGCCAAGCATGAGCACAATTGACCGGCTACAAAGATTTTCATCTGGTGCCTGCCGACCAATAGGAGATGACATGGGAATGGGTATCTGCTGGATTCAAGGGGGGAGAAGCAGCTCATCCAATACTTGCAAGGAATATGATGAGGATGCTAGAGAGACGTTTCCGTGGAGAAGACATAGGAAAGACACATCCCAAAATGGCTCTGTCAATAGGAGGACGATGAGCGCAGGCCCGGAAAAATTAGTATCTGGAAATTTTTGTGATTCACATTGTTCTCCAGGTCATGAATACAAGACTAAGAGTAGTAGCAACAACATACAGCATACTACAAATAAGTTTTTGAAAGATATACCAAAGTTTGTGAAGATAGTAGAAGTTGGTCCAAGGGATGGGCTTCAAAATGAGAAGGGTATGGTACCTACATCTGTGAAGGTGGAATTGATCCATAGACTAGTATCTTCAGGATTGCCTGTGGTTGAGGCTACCAGTTTTGTATCACCTACACGGGTGCCTCAGTTATCTGATGCAGCTGAAGTCATGGAAGCCGTACGTAATTTGGAAGGTGCTAGGTTGCCAGTTTTAACTCCTAATTTGAAAGGGTTTCGAGCAGCTGTTGCGGCTGGAGCAAAGGAAGTGGCAGTCTTTGCATCAGCCTCTGAATTATTTTCAAAATCTAACATCAATTGTAGCATTGAAGAAAGTCTTGATCGTTACCGAGCGGTAACTTCTGCTGCAAAAGAGCTTGCAATCCCTGTTCGAGGGTATGTATCATGTGTTGTTGGTTGTCCAACAGAAGGATCAATTCCTCCATCTAAAGTCGCTTATGTAGCGAAGGAGCTTTATGAGATGGGTTGCTTTGAAATTTCTCTTGGAGACACGATCGGAGTTGCCACGCCCGGGACTGTTGTTCCCATGCTTGAAGCTGTGATGGCTGTCGTTCCGGTGGACAAGCTTGCAGTCCACTTCCATGACACTTATGGACAATCACTTCCAAATATTCTAGTATCCCTCCAAATGGGGATTAGCACAATAGATTCCTCTGTTGCAGGTCTGGGTGGATGTCCATATGCAAAAGGAGCTTCCGGGAATGTTGCAACTGAGGATGTGGTGTACATGCTCCACGGACTTGGCATAAAAACCAATGTGGATTTGGCAAAGGTCATGTCTGCTGGGAACTTCATCAGCAACCATTTGGGGCGCCCATCGGGTTCGAAGACTTCCATTGCCTTCAACCAACTTGCAGCCGATGCTTCCAAGATGTGACACATCTTATTACTCATACATTTACACCTTACATTGAACGAATAATGAAGGGTTTCACTTCAGGAAGCTTTCTTATAGTTTCTATACTATTTTATCTGGCTGAATATGATGCAGCCCATATCTCAAACAACATAACTATTGTCTGTCATGAAGTATCAAAATTTATGGATGCGAGTCCTCCCATGATTTGGAGAGGTTATTATTTATCCCTTTTGTGTCGGTGGTCGGAATGGTTGAGATATTATCATGTTATGGAAAACTTTCTTGAAATTCACCTATTGGCGTTGGAATTCTCGACTCCACCTATTTCATTTATCAATGAAATTATTTCTCTTATCGAAAAAGATATTGTCACATCATATTGTATCGAGTAAAAATGCGTATAAGTATTAAACTACGGTCAAGCCAGAGTAAAAATGTATGTGAAAGAGAGTGTTTGCAGATTCCTTGAAAACAAGATTTTATCTGTTCCAAATCCTTGTTAGTAATTGAATCAGACTTGCACAATGTGGTCTATGGAGAGAAGATAATATATGCAATACAAGACTTATTAAGCAGAAAACCATACTATTCTGTGGAACTGCAACTGAACGACTCCAGAAAGCTCCGGGCAGCATCTGAACTCAGTTCTTCGTTAACCTGTAGTCATATTGATTATAGGATTTTCATTCTAATGTCAATGGAGGATGAAAAACTAAATATCTAATTGCACGACATGTTCTGAATGACTTACAAGTTTATTGTGAATCTGTTGAAATGAGTTACAGAATCTCTCTGCTTCAAGCTTGCCCACCTGCG

Coding sequence (CDS)

ATGTCGAGTTTGGAGGAGCCACTTGGTCTTGACAAGTTGCCAAGCATGAGCACAATTGACCGGCTACAAAGATTTTCATCTGGTGCCTGCCGACCAATAGGAGATGACATGGGAATGGGTATCTGCTGGATTCAAGGGGGGAGAAGCAGCTCATCCAATACTTGCAAGGAATATGATGAGGATGCTAGAGAGACGTTTCCGTGGAGAAGACATAGGAAAGACACATCCCAAAATGGCTCTGTCAATAGGAGGACGATGAGCGCAGGCCCGGAAAAATTAGTATCTGGAAATTTTTGTGATTCACATTGTTCTCCAGGTCATGAATACAAGACTAAGAGTAGTAGCAACAACATACAGCATACTACAAATAAGTTTTTGAAAGATATACCAAAGTTTGTGAAGATAGTAGAAGTTGGTCCAAGGGATGGGCTTCAAAATGAGAAGGGTATGGTACCTACATCTGTGAAGGTGGAATTGATCCATAGACTAGTATCTTCAGGATTGCCTGTGGTTGAGGCTACCAGTTTTGTATCACCTACACGGGTGCCTCAGTTATCTGATGCAGCTGAAGTCATGGAAGCCGTACGTAATTTGGAAGGTGCTAGGTTGCCAGTTTTAACTCCTAATTTGAAAGGGTTTCGAGCAGCTGTTGCGGCTGGAGCAAAGGAAGTGGCAGTCTTTGCATCAGCCTCTGAATTATTTTCAAAATCTAACATCAATTGTAGCATTGAAGAAAGTCTTGATCGTTACCGAGCGGTAACTTCTGCTGCAAAAGAGCTTGCAATCCCTGTTCGAGGGTATGTATCATGTGTTGTTGGTTGTCCAACAGAAGGATCAATTCCTCCATCTAAAGTCGCTTATGTAGCGAAGGAGCTTTATGAGATGGGTTGCTTTGAAATTTCTCTTGGAGACACGATCGGAGTTGCCACGCCCGGGACTGTTGTTCCCATGCTTGAAGCTGTGATGGCTGTCGTTCCGGTGGACAAGCTTGCAGTCCACTTCCATGACACTTATGGACAATCACTTCCAAATATTCTAGTATCCCTCCAAATGGGGATTAGCACAATAGATTCCTCTGTTGCAGGTCTGGGTGGATGTCCATATGCAAAAGGAGCTTCCGGGAATGTTGCAACTGAGGATGTGGTGTACATGCTCCACGGACTTGGCATAAAAACCAATGTGGATTTGGCAAAGGTCATGTCTGCTGGGAACTTCATCAGCAACCATTTGGGGCGCCCATCGGGTTCGAAGACTTCCATTGCCTTCAACCAACTTGCAGCCGATGCTTCCAAGATGTGA

Protein sequence

MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDEDARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQHTTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPTRVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNINCSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSIAFNQLAADASKM
BLAST of Cp4.1LG16g08450 vs. Swiss-Prot
Match: HMGCL_ARATH (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana GN=HMGCL PE=1 SV=2)

HSP 1 Score: 597.0 bits (1538), Expect = 1.6e-169
Identity = 310/434 (71.43%), Postives = 359/434 (82.72%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60
           MSSLEEPL  DKLPSMST+DR+QRFSSGACRP  DD+GMG  WI+G   ++SN+C + D+
Sbjct: 36  MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSCIDDDK 95

Query: 61  D-ARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQ 120
             A+E+FPWRRH +  S+   + R    AG    VSG   +S      +Y T S+ N   
Sbjct: 96  SFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTS 155

Query: 121 HTTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSP 180
           H +NK  K IPKFVKIVEVGPRDGLQNEK +VPTSVKVELI RLVSSGLPVVEATSFVSP
Sbjct: 156 HISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSP 215

Query: 181 TRVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNI 240
             VPQL+DA +VM+AV  L+GARLPVLTPNLKGF+AAV+AGAKEVA+FASASE FS SNI
Sbjct: 216 KWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNI 275

Query: 241 NCSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFE 300
           NC+IEESL RYR V +AAKE ++PVRGYVSCVVGCP EG + PSKVAYV KELY+MGCFE
Sbjct: 276 NCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFE 335

Query: 301 ISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSS 360
           ISLGDTIG+ TPG+VVPMLEAVMAVVP DKLAVHFHDTYGQ+L NILVSLQMGIS +DSS
Sbjct: 336 ISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSS 395

Query: 361 VAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTS 420
           +AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AG+FIS HLGRP+GSK +
Sbjct: 396 IAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAA 455

Query: 421 IAFN-QLAADASKM 433
           +A N ++ ADASK+
Sbjct: 456 VALNRRITADASKI 468

BLAST of Cp4.1LG16g08450 vs. Swiss-Prot
Match: HMGCL_CHICK (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus GN=HMGCL PE=1 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.3e-107
Identity = 197/292 (67.47%), Postives = 237/292 (81.16%), Query Frame = 1

Query: 130 PKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPTRVPQLSDAA 189
           P+ VK+VEVGPRDGLQNEK +VPT VK+ LI  L  +GLPV+EATSFVSP  VPQ++D A
Sbjct: 3   PQRVKVVEVGPRDGLQNEKSVVPTPVKIRLIDMLSETGLPVIEATSFVSPRWVPQMADHA 62

Query: 190 EVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNINCSIEESLDR 249
           EVM+ +  L G   PVLTPNLKGF+AAVAAGAKEV++F +ASELF+K NINCSIEESL+R
Sbjct: 63  EVMQGINKLPGVSYPVLTPNLKGFQAAVAAGAKEVSIFGAASELFTKKNINCSIEESLER 122

Query: 250 YRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEISLGDTIGVA 309
           +  V +AA+  +IPVRGYVSCV+GCP EG+I  +KVA V+K++Y MGC+EISLGD IG+ 
Sbjct: 123 FSEVMNAARAASIPVRGYVSCVLGCPYEGNISAAKVAEVSKKMYSMGCYEISLGDRIGIG 182

Query: 310 TPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPYA 369
           TPG++  ML AVM  VPV  LAVH HDTYGQ+L NILV+LQMG+S +D+SVAGLGGCPYA
Sbjct: 183 TPGSMKEMLAAVMKEVPVGALAVHCHDTYGQALANILVALQMGVSVVDASVAGLGGCPYA 242

Query: 370 KGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSIA 422
           +GASGNVATED+VYML+GLGI T VDL K+M  G FI N L R + SK S A
Sbjct: 243 QGASGNVATEDLVYMLNGLGIHTGVDLQKLMDTGTFICNALNRRTNSKVSQA 294

BLAST of Cp4.1LG16g08450 vs. Swiss-Prot
Match: HMGC2_DANRE (3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic OS=Danio rerio GN=hmgcll1 PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 2.3e-104
Identity = 190/296 (64.19%), Postives = 232/296 (78.38%), Query Frame = 1

Query: 126 LKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPTRVPQL 185
           L  +P +VKIVEVGPRDGLQNEK +VPT VK++LI  L  +GLPV+EATSFVS   V Q+
Sbjct: 36  LPKLPVYVKIVEVGPRDGLQNEKEIVPTEVKIQLIDLLSQTGLPVIEATSFVSSKWVAQM 95

Query: 186 SDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNINCSIEE 245
           +D   V++ ++     R PVLTPN++GF+AAVAAGA EVAVF SASE FS+ NINCSIEE
Sbjct: 96  ADHTAVLKGIKRSPDVRYPVLTPNIQGFQAAVAAGANEVAVFGSASETFSRKNINCSIEE 155

Query: 246 SLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEISLGDT 305
           SL R+  V SAAK+  IPVRGYVSC +GCP EG + PS+V  VAK L+E+GC+E+SLGDT
Sbjct: 156 SLQRFEQVVSAAKQEGIPVRGYVSCALGCPYEGQVKPSQVTKVAKRLFELGCYEVSLGDT 215

Query: 306 IGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGG 365
           IGV T G++  ML  V+  VP   LAVH HDTYGQ+LPNIL++LQMG+S +D+SVAGLGG
Sbjct: 216 IGVGTAGSMAEMLSDVLTEVPAGALAVHCHDTYGQALPNILIALQMGVSVVDASVAGLGG 275

Query: 366 CPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSIA 422
           CP+AKGASGNV+TED++YMLHGLGI+T VDL KVM AG+FI   L R + SK S A
Sbjct: 276 CPFAKGASGNVSTEDLLYMLHGLGIETGVDLLKVMEAGDFICKALNRKTNSKVSQA 331

BLAST of Cp4.1LG16g08450 vs. Swiss-Prot
Match: HMGC2_MOUSE (3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus GN=Hmgcll1 PE=2 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 4.0e-104
Identity = 189/296 (63.85%), Postives = 234/296 (79.05%), Query Frame = 1

Query: 126 LKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPTRVPQL 185
           L  +P++VKIVEVGPRDGLQNEK +VPT +K+ELI++L  +GL V+E TSFVS   VPQ+
Sbjct: 41  LPGLPEYVKIVEVGPRDGLQNEKVIVPTDIKIELINQLSQTGLSVIEVTSFVSSRWVPQM 100

Query: 186 SDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNINCSIEE 245
           +D AEVM  +R   G R PVLTPNL+GF+ AVAAGA E+AVF +ASE FSK NINCSIEE
Sbjct: 101 ADHAEVMRGIRQYPGVRYPVLTPNLQGFQHAVAAGATEIAVFGAASESFSKKNINCSIEE 160

Query: 246 SLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEISLGDT 305
           S+ R++ V S+A+ + IPVRGYVSC +GCP EGSI P KV  V+K LY MGC+EISLGDT
Sbjct: 161 SMGRFQEVISSARHMDIPVRGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDT 220

Query: 306 IGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGG 365
           IGV TPG++  MLE+VM  +P   LAVH HDTYGQ+L NIL +LQMGI+ +DS+V+GLGG
Sbjct: 221 IGVGTPGSMKMMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGG 280

Query: 366 CPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSIA 422
           CPYAKGASGNVATED++YML+G+G+ T VDL KVM AG FI   + + + SK + A
Sbjct: 281 CPYAKGASGNVATEDLIYMLNGMGLNTGVDLYKVMEAGEFICKAVNKTTNSKVAQA 336

BLAST of Cp4.1LG16g08450 vs. Swiss-Prot
Match: HMGCL_BOVIN (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Bos taurus GN=HMGCL PE=2 SV=2)

HSP 1 Score: 378.3 bits (970), Expect = 1.2e-103
Identity = 193/292 (66.10%), Postives = 229/292 (78.42%), Query Frame = 1

Query: 130 PKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPTRVPQLSDAA 189
           PK VKIVEVGPRDGLQNEK +VPT VK++LI  L  +GLPVVEATSFVSP  VPQ++D A
Sbjct: 30  PKQVKIVEVGPRDGLQNEKNIVPTPVKIKLIDMLSEAGLPVVEATSFVSPKWVPQMADHA 89

Query: 190 EVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNINCSIEESLDR 249
           EV++ ++   G   PVLTPN KGF+AAVAAGAKEVA+F +ASELF+K NINCSI+ESL R
Sbjct: 90  EVLKGIQKFPGVNYPVLTPNFKGFQAAVAAGAKEVAIFGAASELFTKKNINCSIDESLQR 149

Query: 250 YRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEISLGDTIGVA 309
           +  +  AA+   I VRGYVSCV+GCP EG I P+KVA V K+LY MGC+EISLGDTIGV 
Sbjct: 150 FDEILKAARAAGISVRGYVSCVLGCPYEGKISPAKVAEVTKKLYSMGCYEISLGDTIGVG 209

Query: 310 TPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPYA 369
           TPG +  ML AV+  VPV  LAVH HDTYGQ+L N L +LQMG+S +DSSVAGLGGCPYA
Sbjct: 210 TPGAMKDMLSAVLQEVPVTALAVHCHDTYGQALANTLTALQMGVSVMDSSVAGLGGCPYA 269

Query: 370 KGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSIA 422
           +GASGN+ATED+VYML GLGI T V+L K++ AG FI   L R + SK + A
Sbjct: 270 QGASGNLATEDLVYMLAGLGIHTGVNLQKLLEAGAFICQALNRRTNSKVAQA 321

BLAST of Cp4.1LG16g08450 vs. TrEMBL
Match: A0A0A0KEI0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G429100 PE=4 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 1.0e-223
Identity = 394/432 (91.20%), Postives = 414/432 (95.83%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSS ACRPIGDDMGMGICWIQGGRSSSSN+CKEYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSSACRPIGDDMGMGICWIQGGRSSSSNSCKEYDE 60

Query: 61  DARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQH 120
           +  +TFPWRRHRK+TSQNG VNRRT SAG +   S NFC+SHCSPGHEYKTKS+S+NIQH
Sbjct: 61  NGGDTFPWRRHRKNTSQNGFVNRRTTSAGSKIFASENFCESHCSPGHEYKTKSTSDNIQH 120

Query: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFLKDIPKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 MTNKFLKDIPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNIN 240
           RVPQLSDAAEVMEAVRNLEGARLPVLTPNLK FRAA+AAGAKEVAVFASASE FSK+NIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKSFRAAIAAGAKEVAVFASASESFSKANIN 240

Query: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEG++ PS VAYVAKELYEMGCFEI
Sbjct: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGAVSPSNVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVM VVPVDKLAVHFHDTYGQSLPNIL+SLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMGVVPVDKLAVHFHDTYGQSLPNILISLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420
           AGLGGCPYAKGA+GNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI+NHLGRPSGSKT+I
Sbjct: 361 AGLGGCPYAKGATGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINNHLGRPSGSKTAI 420

Query: 421 AFNQLAADASKM 433
           A N++AAD+SKM
Sbjct: 421 ALNRVAADSSKM 432

BLAST of Cp4.1LG16g08450 vs. TrEMBL
Match: B9HNI0_POPTR (Putative hydroxymethylglutaryl-CoA lyase family protein OS=Populus trichocarpa GN=POPTR_0009s07280g PE=4 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.0e-186
Identity = 337/432 (78.01%), Postives = 378/432 (87.50%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60
           MSSLEEPLG DKLPSM+TI+R+QRFSSGACRP  DD+GMG CWI+G   SSSN+C+E  E
Sbjct: 1   MSSLEEPLGFDKLPSMNTIERIQRFSSGACRPRADDIGMGHCWIEGRICSSSNSCEEDYE 60

Query: 61  DARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQH 120
             RETFPW+RH +D  Q    NRRTMS+G + +VSG+ CDS   P H+Y +KS+  +I+ 
Sbjct: 61  YTRETFPWKRHTRDPCQG---NRRTMSSGSKNVVSGSSCDSRYFPDHQYSSKSNDKDIRD 120

Query: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFLK IPKFVKIVEVGPRDGLQNEK +VPT VKVELIHRLVSSGLPVVEATSFVSP 
Sbjct: 121 ITNKFLKGIPKFVKIVEVGPRDGLQNEKNIVPTDVKVELIHRLVSSGLPVVEATSFVSPK 180

Query: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNIN 240
            VPQL+DA +VMEAV  LE +RLPVLTPNLKGF AAVAAGAKEVAVFASASE FSKSNIN
Sbjct: 181 WVPQLADARDVMEAVHGLEASRLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNIN 240

Query: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300
           CSI+ESL RYRAVT AAKEL+IPVRGYVSCV+GCP EGSI PSKVAYVAKEL++MGCFEI
Sbjct: 241 CSIKESLARYRAVTHAAKELSIPVRGYVSCVIGCPEEGSIHPSKVAYVAKELHDMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGV TPGTV  MLEAVMAVVPV+KLA+HFHDTYGQSLPNILVSLQMGIS +DSSV
Sbjct: 301 SLGDTIGVGTPGTVALMLEAVMAVVPVEKLAIHFHDTYGQSLPNILVSLQMGISVVDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLG++TNVDLAK++SAG+FI   LGRPSGSKT++
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGVRTNVDLAKLLSAGDFICKQLGRPSGSKTAV 420

Query: 421 AFNQLAADASKM 433
           A +++ ADASK+
Sbjct: 421 ALSRVTADASKI 429

BLAST of Cp4.1LG16g08450 vs. TrEMBL
Match: A0A067LFA7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_22742 PE=4 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 6.5e-186
Identity = 337/435 (77.47%), Postives = 379/435 (87.13%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKE-YD 60
           MSS+EEPLGLDKLPSMSTIDR+QRFSSGACRP  DD+GMG  WI+G   SSSN+C++ Y+
Sbjct: 1   MSSVEEPLGLDKLPSMSTIDRIQRFSSGACRPSADDVGMGNFWIEGRSCSSSNSCEDNYE 60

Query: 61  EDARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNN-- 120
           E  RETFPW+RH +D  Q  S NRRTMS G   +  G   DS C P  +Y +KS SN+  
Sbjct: 61  EYTRETFPWKRHTRDLCQGDSFNRRTMSLGRNHVARGTGQDSRCLPNRQYNSKSKSNDKD 120

Query: 121 IQHTTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFV 180
           +++ TNKFLK IP FVKIVEVGPRDGLQNEK +VPT VK+ELIHRLVSSGLPVVEATSFV
Sbjct: 121 MRNLTNKFLKGIPNFVKIVEVGPRDGLQNEKNIVPTDVKIELIHRLVSSGLPVVEATSFV 180

Query: 181 SPTRVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKS 240
           SP  VPQL+DA +VMEAV+NLEG+RLPVLTPNLKGF AAVAAGAKEVA+FASASE FSKS
Sbjct: 181 SPKWVPQLADARDVMEAVQNLEGSRLPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKS 240

Query: 241 NINCSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGC 300
           NINCSIEESL RYRAVT+AAKE  IPVRGYVSCVVGCP EG+I  SKVAYVAKELY+MGC
Sbjct: 241 NINCSIEESLARYRAVTNAAKERGIPVRGYVSCVVGCPVEGAISLSKVAYVAKELYDMGC 300

Query: 301 FEISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTID 360
           FEISLGDTIGV TPGTVVPMLEAVMAVVP +KLAVHFHDTYGQSLPNIL+SLQMGIST+D
Sbjct: 301 FEISLGDTIGVGTPGTVVPMLEAVMAVVPAEKLAVHFHDTYGQSLPNILISLQMGISTVD 360

Query: 361 SSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSK 420
           SSVAGLGGCPYAKGASGNVATEDVVYML+GLG+KTNVDL K++SAG+FIS +LGR SGSK
Sbjct: 361 SSVAGLGGCPYAKGASGNVATEDVVYMLNGLGVKTNVDLPKLLSAGDFISKYLGRQSGSK 420

Query: 421 TSIAFNQLAADASKM 433
           T++A +++ ADASK+
Sbjct: 421 TAVALSRVTADASKI 435

BLAST of Cp4.1LG16g08450 vs. TrEMBL
Match: W9QRW1_9ROSA (Hydroxymethylglutaryl-CoA lyase OS=Morus notabilis GN=L484_013547 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 2.3e-183
Identity = 337/434 (77.65%), Postives = 376/434 (86.64%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60
           MSSLEEPLG DK+PSMSTIDR+QRFSSGACRP  D+MG G CWI+G   S+SN+C + D 
Sbjct: 1   MSSLEEPLGFDKVPSMSTIDRIQRFSSGACRPREDNMGFGNCWIEGRSCSTSNSCSD-DY 60

Query: 61  DARET--FPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNI 120
           + RET  FPW RH +D SQ  ++NRRT S G  + V+G  C S  S  H+YK KS+  + 
Sbjct: 61  EGRETEIFPWVRHTRDFSQGDTLNRRTSSLGRNQ-VTGTGCSSWYSSDHQYKPKSNDRDK 120

Query: 121 QHTTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVS 180
           Q  TNKFLK +PKFVKIVEVGPRDGLQNEK +VPT+VK+ELIHRLVS GLPVVEATSFVS
Sbjct: 121 QDVTNKFLKSMPKFVKIVEVGPRDGLQNEKNIVPTAVKIELIHRLVSCGLPVVEATSFVS 180

Query: 181 PTRVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSN 240
           P  VPQL+DA +VM AV+NLEGARLPVLTPNLKGF AAVAAGAKEVAVFASASE FSKSN
Sbjct: 181 PKWVPQLADAKDVMGAVQNLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSN 240

Query: 241 INCSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCF 300
           INCSIEESL RYRAVT AAKEL+IPVRGY+SCVVGCP EG+I PSKVAYVAKELY+MGCF
Sbjct: 241 INCSIEESLARYRAVTHAAKELSIPVRGYISCVVGCPVEGAIAPSKVAYVAKELYDMGCF 300

Query: 301 EISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDS 360
           EISL DTIGV TPGTVVPMLEAVMAVVPV+KLAVHFHDTYGQSLPNILVSLQMGISTIDS
Sbjct: 301 EISLADTIGVGTPGTVVPMLEAVMAVVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDS 360

Query: 361 SVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKT 420
           S+AGLGGCPYAKGASGNVATEDVVYML+GLG+KTNVDL K++ AG+FIS  LGRPSGSK+
Sbjct: 361 SIAGLGGCPYAKGASGNVATEDVVYMLNGLGVKTNVDLTKLLKAGDFISKQLGRPSGSKS 420

Query: 421 SIAFNQLAADASKM 433
           +IA ++L ADASK+
Sbjct: 421 AIALSRLTADASKI 432

BLAST of Cp4.1LG16g08450 vs. TrEMBL
Match: V4RY63_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005026mg PE=4 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 1.1e-182
Identity = 334/433 (77.14%), Postives = 371/433 (85.68%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTC-KEYD 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSS ACRP GD MGMG CWI+G   S+SN+  +EYD
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSNACRPSGDGMGMGSCWIEGSSCSTSNSYDEEYD 60

Query: 61  EDARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQ 120
           E  RE FPWRRH +D S   S +RRT+S G    + G+ CD        Y +  +  +I+
Sbjct: 61  EYTREAFPWRRHTRDMSHGDSFSRRTVSLGRNHKMFGSACDI-------YSSNCNDKDIR 120

Query: 121 HTTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSP 180
             TNKFLK IP+ VKIVEVGPRDGLQNEK  VPT VKVELI RLVSSGLPVVEATSFVSP
Sbjct: 121 DITNKFLKGIPRSVKIVEVGPRDGLQNEKNTVPTGVKVELIRRLVSSGLPVVEATSFVSP 180

Query: 181 TRVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNI 240
             VPQL+DA +VMEAVR+LEGARLPVLTPNLKGF AA+AAGAKEVA+FASASE FSKSNI
Sbjct: 181 KWVPQLADARDVMEAVRDLEGARLPVLTPNLKGFEAAIAAGAKEVAIFASASEAFSKSNI 240

Query: 241 NCSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFE 300
           NCSIEESL RYRAV  AAK+L+IPVRGYVSCVVGCP EG+IPPSKVAYVAKEL++MGCFE
Sbjct: 241 NCSIEESLVRYRAVARAAKDLSIPVRGYVSCVVGCPVEGAIPPSKVAYVAKELHDMGCFE 300

Query: 301 ISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSS 360
           ISLGDTIGV TPGTVVPMLEAVMAVVPV+KLAVHFHDTYGQSLPNIL+SLQMGIST+D S
Sbjct: 301 ISLGDTIGVGTPGTVVPMLEAVMAVVPVEKLAVHFHDTYGQSLPNILISLQMGISTVDCS 360

Query: 361 VAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTS 420
           VAGLGGCPYAKGASGNVATEDVVYML GLG++TNVDL K+M AG+FI+ HLGRPSGSKT+
Sbjct: 361 VAGLGGCPYAKGASGNVATEDVVYMLSGLGVETNVDLRKLMLAGDFINKHLGRPSGSKTA 420

Query: 421 IAFNQLAADASKM 433
           IA N++AADASK+
Sbjct: 421 IALNRIAADASKI 426

BLAST of Cp4.1LG16g08450 vs. TAIR10
Match: AT2G26800.2 (AT2G26800.2 Aldolase superfamily protein)

HSP 1 Score: 597.0 bits (1538), Expect = 9.0e-171
Identity = 310/434 (71.43%), Postives = 359/434 (82.72%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60
           MSSLEEPL  DKLPSMST+DR+QRFSSGACRP  DD+GMG  WI+G   ++SN+C + D+
Sbjct: 36  MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSCIDDDK 95

Query: 61  D-ARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQ 120
             A+E+FPWRRH +  S+   + R    AG    VSG   +S      +Y T S+ N   
Sbjct: 96  SFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTS 155

Query: 121 HTTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSP 180
           H +NK  K IPKFVKIVEVGPRDGLQNEK +VPTSVKVELI RLVSSGLPVVEATSFVSP
Sbjct: 156 HISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSP 215

Query: 181 TRVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNI 240
             VPQL+DA +VM+AV  L+GARLPVLTPNLKGF+AAV+AGAKEVA+FASASE FS SNI
Sbjct: 216 KWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNI 275

Query: 241 NCSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFE 300
           NC+IEESL RYR V +AAKE ++PVRGYVSCVVGCP EG + PSKVAYV KELY+MGCFE
Sbjct: 276 NCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFE 335

Query: 301 ISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSS 360
           ISLGDTIG+ TPG+VVPMLEAVMAVVP DKLAVHFHDTYGQ+L NILVSLQMGIS +DSS
Sbjct: 336 ISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSS 395

Query: 361 VAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTS 420
           +AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AG+FIS HLGRP+GSK +
Sbjct: 396 IAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAA 455

Query: 421 IAFN-QLAADASKM 433
           +A N ++ ADASK+
Sbjct: 456 VALNRRITADASKI 468

BLAST of Cp4.1LG16g08450 vs. NCBI nr
Match: gi|659097451|ref|XP_008449633.1| (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 784.6 bits (2025), Expect = 8.6e-224
Identity = 396/432 (91.67%), Postives = 416/432 (96.30%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSS ACRPIGDDMGMGICWIQGGRSSSSN+CKEYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSSACRPIGDDMGMGICWIQGGRSSSSNSCKEYDE 60

Query: 61  DARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQH 120
           DA +TFPWRRHRK+TSQNGSVNRRTMSAG +K  S NFC+ HCS GHEYKTKS+S+NIQH
Sbjct: 61  DAGDTFPWRRHRKNTSQNGSVNRRTMSAGSKKFASENFCEPHCSLGHEYKTKSTSDNIQH 120

Query: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFLKDIPKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 MTNKFLKDIPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNIN 240
           RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAA+ AGAKEVAVFASASE FSK+NIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAITAGAKEVAVFASASESFSKANIN 240

Query: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEG++ PSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGAVSPSKVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVV MLEAVMAVVPVDKLAVHFHDTYGQSLPNIL+SLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVAMLEAVMAVVPVDKLAVHFHDTYGQSLPNILISLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420
           AGLGGCPYAKGA+GNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI++HLGR SGSKT+I
Sbjct: 361 AGLGGCPYAKGATGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINSHLGRLSGSKTAI 420

Query: 421 AFNQLAADASKM 433
           A N++AAD+SKM
Sbjct: 421 ALNRVAADSSKM 432

BLAST of Cp4.1LG16g08450 vs. NCBI nr
Match: gi|659097449|ref|XP_008449632.1| (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 784.6 bits (2025), Expect = 8.6e-224
Identity = 396/432 (91.67%), Postives = 416/432 (96.30%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSS ACRPIGDDMGMGICWIQGGRSSSSN+CKEYDE
Sbjct: 37  MSSLEEPLGLDKLPSMSTIDRIQRFSSSACRPIGDDMGMGICWIQGGRSSSSNSCKEYDE 96

Query: 61  DARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQH 120
           DA +TFPWRRHRK+TSQNGSVNRRTMSAG +K  S NFC+ HCS GHEYKTKS+S+NIQH
Sbjct: 97  DAGDTFPWRRHRKNTSQNGSVNRRTMSAGSKKFASENFCEPHCSLGHEYKTKSTSDNIQH 156

Query: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFLKDIPKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 157 MTNKFLKDIPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 216

Query: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNIN 240
           RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAA+ AGAKEVAVFASASE FSK+NIN
Sbjct: 217 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAITAGAKEVAVFASASESFSKANIN 276

Query: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEG++ PSKVAYVAKELYEMGCFEI
Sbjct: 277 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGAVSPSKVAYVAKELYEMGCFEI 336

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVV MLEAVMAVVPVDKLAVHFHDTYGQSLPNIL+SLQMGISTIDSSV
Sbjct: 337 SLGDTIGVATPGTVVAMLEAVMAVVPVDKLAVHFHDTYGQSLPNILISLQMGISTIDSSV 396

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420
           AGLGGCPYAKGA+GNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI++HLGR SGSKT+I
Sbjct: 397 AGLGGCPYAKGATGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINSHLGRLSGSKTAI 456

Query: 421 AFNQLAADASKM 433
           A N++AAD+SKM
Sbjct: 457 ALNRVAADSSKM 468

BLAST of Cp4.1LG16g08450 vs. NCBI nr
Match: gi|449444847|ref|XP_004140185.1| (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 783.9 bits (2023), Expect = 1.5e-223
Identity = 394/432 (91.20%), Postives = 414/432 (95.83%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSS ACRPIGDDMGMGICWIQGGRSSSSN+CKEYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSSACRPIGDDMGMGICWIQGGRSSSSNSCKEYDE 60

Query: 61  DARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQH 120
           +  +TFPWRRHRK+TSQNG VNRRT SAG +   S NFC+SHCSPGHEYKTKS+S+NIQH
Sbjct: 61  NGGDTFPWRRHRKNTSQNGFVNRRTTSAGSKIFASENFCESHCSPGHEYKTKSTSDNIQH 120

Query: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFLKDIPKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 121 MTNKFLKDIPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNIN 240
           RVPQLSDAAEVMEAVRNLEGARLPVLTPNLK FRAA+AAGAKEVAVFASASE FSK+NIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKSFRAAIAAGAKEVAVFASASESFSKANIN 240

Query: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEG++ PS VAYVAKELYEMGCFEI
Sbjct: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGAVSPSNVAYVAKELYEMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVM VVPVDKLAVHFHDTYGQSLPNIL+SLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMGVVPVDKLAVHFHDTYGQSLPNILISLQMGISTIDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420
           AGLGGCPYAKGA+GNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI+NHLGRPSGSKT+I
Sbjct: 361 AGLGGCPYAKGATGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINNHLGRPSGSKTAI 420

Query: 421 AFNQLAADASKM 433
           A N++AAD+SKM
Sbjct: 421 ALNRVAADSSKM 432

BLAST of Cp4.1LG16g08450 vs. NCBI nr
Match: gi|778716852|ref|XP_011657605.1| (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 783.9 bits (2023), Expect = 1.5e-223
Identity = 394/432 (91.20%), Postives = 414/432 (95.83%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60
           MSSLEEPLGLDKLPSMSTIDR+QRFSS ACRPIGDDMGMGICWIQGGRSSSSN+CKEYDE
Sbjct: 37  MSSLEEPLGLDKLPSMSTIDRIQRFSSSACRPIGDDMGMGICWIQGGRSSSSNSCKEYDE 96

Query: 61  DARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQH 120
           +  +TFPWRRHRK+TSQNG VNRRT SAG +   S NFC+SHCSPGHEYKTKS+S+NIQH
Sbjct: 97  NGGDTFPWRRHRKNTSQNGFVNRRTTSAGSKIFASENFCESHCSPGHEYKTKSTSDNIQH 156

Query: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFLKDIPKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGLPVVEATSFVSPT
Sbjct: 157 MTNKFLKDIPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 216

Query: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNIN 240
           RVPQLSDAAEVMEAVRNLEGARLPVLTPNLK FRAA+AAGAKEVAVFASASE FSK+NIN
Sbjct: 217 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKSFRAAIAAGAKEVAVFASASESFSKANIN 276

Query: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300
           CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEG++ PS VAYVAKELYEMGCFEI
Sbjct: 277 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGAVSPSNVAYVAKELYEMGCFEI 336

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGVATPGTVVPMLEAVM VVPVDKLAVHFHDTYGQSLPNIL+SLQMGISTIDSSV
Sbjct: 337 SLGDTIGVATPGTVVPMLEAVMGVVPVDKLAVHFHDTYGQSLPNILISLQMGISTIDSSV 396

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420
           AGLGGCPYAKGA+GNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFI+NHLGRPSGSKT+I
Sbjct: 397 AGLGGCPYAKGATGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFINNHLGRPSGSKTAI 456

Query: 421 AFNQLAADASKM 433
           A N++AAD+SKM
Sbjct: 457 ALNRVAADSSKM 468

BLAST of Cp4.1LG16g08450 vs. NCBI nr
Match: gi|743881150|ref|XP_011036394.1| (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial [Populus euphratica])

HSP 1 Score: 661.8 bits (1706), Expect = 8.4e-187
Identity = 337/432 (78.01%), Postives = 379/432 (87.73%), Query Frame = 1

Query: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60
           MSSLEEPLG DKLPSM+TI+R+QRFSSGACRP  DDMGMG CWI+G   SSSN+C+E  E
Sbjct: 1   MSSLEEPLGFDKLPSMNTIERIQRFSSGACRPRADDMGMGHCWIEGRTCSSSNSCEEDYE 60

Query: 61  DARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQH 120
             RETFPW+RH +D+ Q    NRR++S+G + +VSG+ CDS   P H+Y +KS+  +I+ 
Sbjct: 61  YTRETFPWKRHTRDSCQG---NRRSVSSGSKNMVSGSSCDSRYFPDHQYSSKSNDKDIRG 120

Query: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180
            TNKFLK IPKFVKIVEVGPRDGLQNEK +VPT VKVELIHRLVSSGLPVVEATSFVSP 
Sbjct: 121 ITNKFLKGIPKFVKIVEVGPRDGLQNEKNIVPTDVKVELIHRLVSSGLPVVEATSFVSPK 180

Query: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNIN 240
            VPQL+DA +VMEAV  LE +RLPVLTPNLKGF AAVAAGAKEVAVFASASE FSKSNIN
Sbjct: 181 WVPQLADARDVMEAVHGLEASRLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNIN 240

Query: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300
           CSIEESL RYRAVT AAKEL+IPVRGYVSCV+GCP EGSI PSKVAYVAKEL++MGCFEI
Sbjct: 241 CSIEESLARYRAVTHAAKELSIPVRGYVSCVIGCPEEGSIHPSKVAYVAKELHDMGCFEI 300

Query: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360
           SLGDTIGV TPGTV  MLEAVMAVVPV+KLA+HFHDTYGQSLPNILVSLQMGIS +DSSV
Sbjct: 301 SLGDTIGVGTPGTVALMLEAVMAVVPVEKLAIHFHDTYGQSLPNILVSLQMGISVVDSSV 360

Query: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420
           AGLGGCPYAKGASGNVATEDVVYMLHGLG++TNVDLAK++SAG+FI   LGRPSGSKT++
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGVRTNVDLAKLLSAGDFICKQLGRPSGSKTAM 420

Query: 421 AFNQLAADASKM 433
           A +++ ADASK+
Sbjct: 421 ALSRVTADASKI 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
HMGCL_ARATH1.6e-16971.43Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana GN=HMGCL ... [more]
HMGCL_CHICK1.3e-10767.47Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus GN=HMGCL PE=1 SV... [more]
HMGC2_DANRE2.3e-10464.193-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic OS=Danio rerio GN=hmgcll... [more]
HMGC2_MOUSE4.0e-10463.853-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus GN=Hmgcl... [more]
HMGCL_BOVIN1.2e-10366.10Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Bos taurus GN=HMGCL PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KEI0_CUCSA1.0e-22391.20Uncharacterized protein OS=Cucumis sativus GN=Csa_6G429100 PE=4 SV=1[more]
B9HNI0_POPTR1.0e-18678.01Putative hydroxymethylglutaryl-CoA lyase family protein OS=Populus trichocarpa G... [more]
A0A067LFA7_JATCU6.5e-18677.47Uncharacterized protein OS=Jatropha curcas GN=JCGZ_22742 PE=4 SV=1[more]
W9QRW1_9ROSA2.3e-18377.65Hydroxymethylglutaryl-CoA lyase OS=Morus notabilis GN=L484_013547 PE=4 SV=1[more]
V4RY63_9ROSI1.1e-18277.14Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005026mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G26800.29.0e-17171.43 Aldolase superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659097451|ref|XP_008449633.1|8.6e-22491.67PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucumis me... [more]
gi|659097449|ref|XP_008449632.1|8.6e-22491.67PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Cucumis me... [more]
gi|449444847|ref|XP_004140185.1|1.5e-22391.20PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2 [Cucumis sa... [more]
gi|778716852|ref|XP_011657605.1|1.5e-22391.20PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Cucumis sa... [more]
gi|743881150|ref|XP_011036394.1|8.4e-18778.01PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial [Populus euphratica][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0004419hydroxymethylglutaryl-CoA lyase activity
Vocabulary: INTERPRO
TermDefinition
IPR013785Aldolase_TIM
IPR000891PYR_CT
IPR000138HMG_CoA_lyase_AS
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046950 cellular ketone body metabolic process
biological_process GO:0006550 isoleucine catabolic process
biological_process GO:0006552 leucine catabolic process
biological_process GO:0006574 valine catabolic process
biological_process GO:0046951 ketone body biosynthetic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004419 hydroxymethylglutaryl-CoA lyase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g08450.1Cp4.1LG16g08450.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000138Hydroxymethylglutaryl-CoA lyase, active sitePROSITEPS01062HMG_COA_LYASEcoord: 359..368
scor
IPR000891Pyruvate carboxyltransferasePFAMPF00682HMGL-likecoord: 133..406
score: 5.9
IPR000891Pyruvate carboxyltransferasePROFILEPS50991PYR_CTcoord: 133..400
score: 34
IPR013785Aldolase-type TIM barrelGENE3DG3DSA:3.20.20.70coord: 131..417
score: 3.2
NoneNo IPR availablePANTHERPTHR10277HOMOCITRATE SYNTHASE-RELATEDcoord: 95..432
score: 6.0E
NoneNo IPR availablePANTHERPTHR10277:SF40PROTEIN Y71G12B.10coord: 95..432
score: 6.0E
NoneNo IPR availableunknownSSF51569Aldolasecoord: 128..420
score: 2.64