Cp4.1LG16g08260 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG16g08260
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionU-box domain-containing family protein
LocationCp4.1LG16 : 7940610 .. 7941851 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAATGGAAGAAGACGGCGGCGGCGATCTTGAAGTCCCCTCCTATTTCCTCTGCCCAATTTCCCTCCAATTGATGAGAGATCCGGTGACCATCTCCACCGGCATCACCTACGACCGATCCAACATCGAGAAATGGCTCTTCTCATGCAACAAGAGAACTTGCCCCATCACTAAACAGCCCTTGTCCGATCCCGATTTGGTAACCCCAAACCACACCCTTCGCCGTTTGATCCAAGCTTGGTGCACCCTCAACGCCTCTCATGGAGTTGAACGAATTCCAACTCCCAAGTCACCCATTGACAAAACCCATGTGGCCAAGATCCTCAAACAGGCCAAGAACTTCCCCAGTTCCAACCACAAGTGCCTCCTTGCACTCAAAGCCATTGCCTTGGAGAGTGAAAGAAATTGCACCCTTGTGGCTCAATCCGATGGAGCTTTTGAGTTTGTAGCCGCCGTTATTAAGAGTGGTGGTGATAAGAACATTGGGTCCATTGAATTGGCGGTGGAAATTCTGTTCCACATCAAATCCTCGGAAGCCCATCTCAAGAATCTCGTAAATGGCGATGGTAACTTCATAAACTCTCTTACTTTTGTCCTCAAAAATGGGAACTGCCAATCTAGAGCCTACGCCATAATGTTGCTCAAATCAAGCTTGGAACTTGCTGATCCAATTCGGTTGATGAGCATTGAGAGGGAACTTCTTAGTGAAATTGTGCGCGTTATACATGATCATATATCTCACCAAGCCTCCAAATCCGCGTTGAAGGTTCTAGCCGAGGTGTGCCCTTGGGGGAGGAACCGTGTGAAAGCGGTCGAGGGCGGGGCAGTCGGCGTGTTGGTGGAGCTGCTTTTGAACTCGACCGAGAGGCGGTGGTCGGAGATAGGGCTGGTGATTTTGGATCAGCTGTGTGGGTGCGCAGAGGGACGAGAGAAGCTGATGGAACACAGTGCTGGAGTTGCAATTGTGTCGAAGAAAATACTTAGGGTTTCGGCAATGGCGAGCGATCGAGCGGTGAGGATATTGTCGTCGATATGTCGGTTTTCGGCGTCGGTAAAGGTAGTTCAAGAAATGTTGGAGGTGGGAGTCGTGGCAAAACTGTGTTTGGTGTTGCAAATGGATTGTAGCTCGAAGACGAGAGAGAAGGCGAGGGATATACTAAAGTTGCATTCGAGGGTTTGGAGTAATTCTTCTTGTATTCCGCCACATTTGGCGTCTGCTTATCCATCGTCACCGTCTTGA

mRNA sequence

ATGAGAATGGAAGAAGACGGCGGCGGCGATCTTGAAGTCCCCTCCTATTTCCTCTGCCCAATTTCCCTCCAATTGATGAGAGATCCGGTGACCATCTCCACCGGCATCACCTACGACCGATCCAACATCGAGAAATGGCTCTTCTCATGCAACAAGAGAACTTGCCCCATCACTAAACAGCCCTTGTCCGATCCCGATTTGGTAACCCCAAACCACACCCTTCGCCGTTTGATCCAAGCTTGGTGCACCCTCAACGCCTCTCATGGAGTTGAACGAATTCCAACTCCCAAGTCACCCATTGACAAAACCCATGTGGCCAAGATCCTCAAACAGGCCAAGAACTTCCCCAGTTCCAACCACAAGTGCCTCCTTGCACTCAAAGCCATTGCCTTGGAGAGTGAAAGAAATTGCACCCTTGTGGCTCAATCCGATGGAGCTTTTGAGTTTGTAGCCGCCGTTATTAAGAGTGGTGGTGATAAGAACATTGGGTCCATTGAATTGGCGGTGGAAATTCTGTTCCACATCAAATCCTCGGAAGCCCATCTCAAGAATCTCGTAAATGGCGATGGTAACTTCATAAACTCTCTTACTTTTGTCCTCAAAAATGGGAACTGCCAATCTAGAGCCTACGCCATAATGTTGCTCAAATCAAGCTTGGAACTTGCTGATCCAATTCGGTTGATGAGCATTGAGAGGGAACTTCTTAGTGAAATTGTGCGCGTTATACATGATCATATATCTCACCAAGCCTCCAAATCCGCGTTGAAGGTTCTAGCCGAGGTGTGCCCTTGGGGGAGGAACCGTGTGAAAGCGGTCGAGGGCGGGGCAGTCGGCGTGTTGGTGGAGCTGCTTTTGAACTCGACCGAGAGGCGGTGGTCGGAGATAGGGCTGGTGATTTTGGATCAGCTGTGTGGGTGCGCAGAGGGACGAGAGAAGCTGATGGAACACAGTGCTGGAGTTGCAATTGTGTCGAAGAAAATACTTAGGGTTTCGGCAATGGCGAGCGATCGAGCGGTGAGGATATTGTCGTCGATATGTCGGTTTTCGGCGTCGGTAAAGGTAGTTCAAGAAATGTTGGAGGTGGGAGTCGTGGCAAAACTGTGTTTGGTGTTGCAAATGGATTGTAGCTCGAAGACGAGAGAGAAGGCGAGGGATATACTAAAGTTGCATTCGAGGGTTTGGAGTAATTCTTCTTGTATTCCGCCACATTTGGCGTCTGCTTATCCATCGTCACCGTCTTGA

Coding sequence (CDS)

ATGAGAATGGAAGAAGACGGCGGCGGCGATCTTGAAGTCCCCTCCTATTTCCTCTGCCCAATTTCCCTCCAATTGATGAGAGATCCGGTGACCATCTCCACCGGCATCACCTACGACCGATCCAACATCGAGAAATGGCTCTTCTCATGCAACAAGAGAACTTGCCCCATCACTAAACAGCCCTTGTCCGATCCCGATTTGGTAACCCCAAACCACACCCTTCGCCGTTTGATCCAAGCTTGGTGCACCCTCAACGCCTCTCATGGAGTTGAACGAATTCCAACTCCCAAGTCACCCATTGACAAAACCCATGTGGCCAAGATCCTCAAACAGGCCAAGAACTTCCCCAGTTCCAACCACAAGTGCCTCCTTGCACTCAAAGCCATTGCCTTGGAGAGTGAAAGAAATTGCACCCTTGTGGCTCAATCCGATGGAGCTTTTGAGTTTGTAGCCGCCGTTATTAAGAGTGGTGGTGATAAGAACATTGGGTCCATTGAATTGGCGGTGGAAATTCTGTTCCACATCAAATCCTCGGAAGCCCATCTCAAGAATCTCGTAAATGGCGATGGTAACTTCATAAACTCTCTTACTTTTGTCCTCAAAAATGGGAACTGCCAATCTAGAGCCTACGCCATAATGTTGCTCAAATCAAGCTTGGAACTTGCTGATCCAATTCGGTTGATGAGCATTGAGAGGGAACTTCTTAGTGAAATTGTGCGCGTTATACATGATCATATATCTCACCAAGCCTCCAAATCCGCGTTGAAGGTTCTAGCCGAGGTGTGCCCTTGGGGGAGGAACCGTGTGAAAGCGGTCGAGGGCGGGGCAGTCGGCGTGTTGGTGGAGCTGCTTTTGAACTCGACCGAGAGGCGGTGGTCGGAGATAGGGCTGGTGATTTTGGATCAGCTGTGTGGGTGCGCAGAGGGACGAGAGAAGCTGATGGAACACAGTGCTGGAGTTGCAATTGTGTCGAAGAAAATACTTAGGGTTTCGGCAATGGCGAGCGATCGAGCGGTGAGGATATTGTCGTCGATATGTCGGTTTTCGGCGTCGGTAAAGGTAGTTCAAGAAATGTTGGAGGTGGGAGTCGTGGCAAAACTGTGTTTGGTGTTGCAAATGGATTGTAGCTCGAAGACGAGAGAGAAGGCGAGGGATATACTAAAGTTGCATTCGAGGGTTTGGAGTAATTCTTCTTGTATTCCGCCACATTTGGCGTCTGCTTATCCATCGTCACCGTCTTGA

Protein sequence

MRMEEDGGGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEAHLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVILDQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSSPS
BLAST of Cp4.1LG16g08260 vs. Swiss-Prot
Match: PUB23_ARATH (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana GN=PUB23 PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 7.4e-116
Identity = 205/406 (50.49%), Postives = 304/406 (74.88%), Query Frame = 1

Query: 10  DLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVT 69
           ++E+P +FLCPISL++M+DPV +STGITYDR +IEKWLF+  K +CP+TKQ ++D DL T
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADL-T 68

Query: 70  PNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKAI 129
           PNHTLRRLIQ+WCTLNAS+GVERIPTP+ PI K+ + K+++ + +   +  KCL  L+ I
Sbjct: 69  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 128

Query: 130 ALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSI-ELAVEILFHIKSSEAHLKNLVNG 189
             E+  N   + ++ G  EF+A ++ +  D   GS+ + A+ +L+H+++SE  LKNL+N 
Sbjct: 129 VSENATNKRCL-EAAGVPEFLANIVSN--DSENGSLTDEALNLLYHLETSETVLKNLLNN 188

Query: 190 --DGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVRVIHDHI 249
             D N + SLT +++ G  +SR YA +LLK+ LE+ADP++ M+++ E+ +E+V+++ D I
Sbjct: 189 KKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRI 248

Query: 250 SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLN---STERRWSEIGLVILDQL 309
           S +A+K+A+ +L  +CPWGRNR KAVE G + V++ELL++   ++ERR  E+ +V+LD L
Sbjct: 249 SQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLL 308

Query: 310 CGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGV 369
           C CAEGR + + H A +A+V KKILRVS  ASDRAVR+L S+ RF A+  ++ EML++GV
Sbjct: 309 CQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGV 368

Query: 370 VAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYP 410
           VAKLCLVLQ+ C  KT+EKA+++LKLH+RVW +S C+P ++  AYP
Sbjct: 369 VAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of Cp4.1LG16g08260 vs. Swiss-Prot
Match: PUB22_ARATH (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana GN=PUB22 PE=1 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 2.2e-115
Identity = 207/433 (47.81%), Postives = 307/433 (70.90%), Query Frame = 1

Query: 10  DLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVT 69
           ++E+PS+FLCPISL +M+DPV +STGITYDR +IEKWLFS  K +CP+TKQ +++ DL T
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDL-T 63

Query: 70  PNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKAI 129
           PNHTLRRLIQ+WCTLNAS+G+ERIPTPK PI K+ + K++K++ +   +  KCL  L+ I
Sbjct: 64  PNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQI 123

Query: 130 ALESERN-------------CTLVAQSDGAFEFVAAVIKSGG--------------DKNI 189
             E+  N               +V+ S   +   ++ + S                D + 
Sbjct: 124 VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 183

Query: 190 GSIELAVEILFHIKSSEAHLKNLVNG--DGNFINSLTFVLKNGNCQSRAYAIMLLKSSLE 249
             ++ A+ +L+H+ +SE  LK+L+N     N + +LT +++ G  +SRAYA +LLK  LE
Sbjct: 184 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLE 243

Query: 250 LADPIRLMSIERELLSEIVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVL 309
           +ADP++++ +EREL  E+++++HD ISH+A++SA+++L   CPWGRNR KAVEGG + ++
Sbjct: 244 VADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMI 303

Query: 310 VELLLN---STERRWSEIGLVILDQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDR 369
           +ELL++   S+ERR SE+ +V+LD LC CAEGR + + H A +A+VSKKILRVS + S+R
Sbjct: 304 IELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSER 363

Query: 370 AVRILSSICRFSASVKVVQEMLEVGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNS 411
           AVR+L S+ RF A+  ++QEML++GVVAKLCLVLQ+ C +KT+EKA+++LKLH+RVW  S
Sbjct: 364 AVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHARVWRES 423

BLAST of Cp4.1LG16g08260 vs. Swiss-Prot
Match: PUB25_ARATH (U-box domain-containing protein 25 OS=Arabidopsis thaliana GN=PUB25 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.7e-51
Identity = 132/394 (33.50%), Postives = 210/394 (53.30%), Query Frame = 1

Query: 11  LEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVTP 70
           +++P +F CPISL+LM+DPVT+ TG TYDR++IE W+   N  TCP+T+ PLSD  L+ P
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLI-P 71

Query: 71  NHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPS------SNHKCLL 130
           NHTLRRLIQ WC  N S+GVERIPTPK P D T V  +L QA           S    L 
Sbjct: 72  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 131

Query: 131 ALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEAHLKN 190
            L+  A +S++N  L+A  +     +  +        + S  LA+ ++  I      +  
Sbjct: 132 RLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFVS- 191

Query: 191 LVNGDGNFINSLTFVLKNGNCQSRAYA---IMLLKSSLELADPIRLMSIERELLSEIVRV 250
            ++ D   +  LT +L + + ++R  A   I ++ +  + AD    +S    +   ++ +
Sbjct: 192 -ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLDL 251

Query: 251 IHDHI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVIL 310
           + + I S +A K  +K L  +C     R  A+  GA  +L++ L    +R  +E  L  +
Sbjct: 252 LRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATV 311

Query: 311 DQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 370
           + LC   EG     EH+  V ++ K ILRVS  A++ A   L ++C  +A  +  +E   
Sbjct: 312 ELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC--TAEERWREEAAG 371

Query: 371 VGVVAKLCLVLQMDCSSKTREKARDILKLHSRVW 395
            GVV +L L++Q +C+ + ++KA+ +LKL    W
Sbjct: 372 AGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of Cp4.1LG16g08260 vs. Swiss-Prot
Match: PUB26_ARATH (U-box domain-containing protein 26 OS=Arabidopsis thaliana GN=PUB26 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.4e-50
Identity = 138/404 (34.16%), Postives = 215/404 (53.22%), Query Frame = 1

Query: 11  LEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVTP 70
           +++P +F CPISL LM DPVTISTG TYDR++I+ W+   N  TCP+T+  LSD  L+ P
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNT-TCPVTRVALSDFTLI-P 71

Query: 71  NHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPS------SNHKCLL 130
           NHTLRRLIQ WC  N S+GVERIPTPK P D   V  +L QA           S    + 
Sbjct: 72  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIR 131

Query: 131 ALKAIALESERNCTLVAQSDGAFEFV----AAVIKSGGDKNIGSIELAVEILFHIKSSEA 190
            L+ +A +SE+N  L+A  +     V    A +  +     + S  LA+ +L H+  +E 
Sbjct: 132 RLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVSESLALLVLLHMTETEC 191

Query: 191 HLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRL---MSIERELLSE 250
                V  D + +  +T +L + + + R  A  L++  L  A  + L   +S    +   
Sbjct: 192 ---EAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEG 251

Query: 251 IVRVIHDHI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIG 310
           ++ ++ + I S +A K  +K +  +C   + R  A+  GA G+L++ L    +R  +E G
Sbjct: 252 VLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTERG 311

Query: 311 LVILDQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQ 370
           L  ++ LC   EG     EH+  V ++ K ILRVS  A++ A   L ++C  +A  +   
Sbjct: 312 LATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC--TAEERCRD 371

Query: 371 EMLEVGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCI 401
           E    G+V +L L++Q DC+ + + KA+ +LKL    W + S +
Sbjct: 372 EAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTV 408

BLAST of Cp4.1LG16g08260 vs. Swiss-Prot
Match: PUB21_ARATH (U-box domain-containing protein 21 OS=Arabidopsis thaliana GN=PUB21 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.5e-39
Identity = 116/407 (28.50%), Postives = 217/407 (53.32%), Query Frame = 1

Query: 4   EEDGGGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLS 63
           E +   ++ +P  F CPIS+ LM+DPV ISTGITYDR +IE W+ S NK TCP+T   L+
Sbjct: 22  ESEPESEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNK-TCPVTNTVLT 81

Query: 64  DPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFP--SSNHK 123
             D + PNHT+R++IQ WC    S  ++RIPTP+ P+    V +I ++  +        K
Sbjct: 82  TFDQI-PNHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEK 141

Query: 124 C---LLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSI--ELAVEILFHIK 183
           C   +  +K +  ESE+N   V ++   +       K  GD+ +  +  E+   + +   
Sbjct: 142 CGVIIEKIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKLTFMLNEILSLLTWMFP 201

Query: 184 SSEAHLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADP-IRLMSIERELL 243
                +  L +        +  +LK+ +   R  A  ++K  L L +  +   ++E  + 
Sbjct: 202 IGLEGISKLASATS--FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVA 261

Query: 244 SEIVRVIHDHISHQASKSALKVLAE-VCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSE 303
             +V++I D +S  ++KS+L  + + V        + +E G V + VE+++++ E    E
Sbjct: 262 EALVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDA-ENSVCE 321

Query: 304 IGLVILDQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKV 363
             L +LD +C    GRE++ +++  + ++ KKI +VS +A+  ++ ++  + +   +V  
Sbjct: 322 KALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTV-A 381

Query: 364 VQEMLEVGVVAKLCLVLQMDCSSKTREKARDILK-LHSRVWSNSSCI 401
           V++ + +G   K+ LVLQ+    +T+EKA ++LK +++++   S C+
Sbjct: 382 VEDAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCV 422

BLAST of Cp4.1LG16g08260 vs. TrEMBL
Match: A0A0A0KJY2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G426370 PE=4 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 3.1e-201
Identity = 359/404 (88.86%), Postives = 383/404 (94.80%), Query Frame = 1

Query: 9   GDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLS-DPDL 68
           G++EVPSYFLCPISLQLMRDPVTISTGITYDRS+IE WLFS NK  CPITKQPLS DPDL
Sbjct: 9   GEIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEMWLFSFNKTVCPITKQPLSSDPDL 68

Query: 69  VTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALK 128
           +TPNHTLRRLIQ+WCTLNAS+G+ERIPTPKSPIDKTH+AKILKQA++FP S HKCLLALK
Sbjct: 69  LTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQSFPDSTHKCLLALK 128

Query: 129 AIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEAHLKNLVN 188
           AIALE+ERN  LVAQSDGAF+F+A VIKSGG  NI SIELAVEILFHIKSS+ HLKNLVN
Sbjct: 129 AIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQTHLKNLVN 188

Query: 189 GDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVRVIHDHIS 248
           GD +FINSLTFVL+NGNCQSRAYA+MLLKSSLELADPIRLMSIERELL EIVRVIHDHIS
Sbjct: 189 GDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLREIVRVIHDHIS 248

Query: 249 HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVILDQLCGCA 308
           HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSE+GLVILDQLCGCA
Sbjct: 249 HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVILDQLCGCA 308

Query: 309 EGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVAKL 368
           EGREKL+ H+AG+A+VSKKILRVSAMASDRAVRILSSICRFSAS KV+QEMLEVGVVAKL
Sbjct: 309 EGREKLVAHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVMQEMLEVGVVAKL 368

Query: 369 CLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 412
           CLVLQMDCS KTREKARD LKLHSRVWSNSSCIPPHL SAYPS+
Sbjct: 369 CLVLQMDCSMKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 412

BLAST of Cp4.1LG16g08260 vs. TrEMBL
Match: W9QTE7_9ROSA (E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_017126 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 7.7e-144
Identity = 256/404 (63.37%), Postives = 329/404 (81.44%), Query Frame = 1

Query: 10  DLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLV- 69
           +++VP  FLCPISLQLMRDPVT+STGITYDR NIE WLFSC   TCP+TKQPL+D DL+ 
Sbjct: 3   EIDVPYLFLCPISLQLMRDPVTVSTGITYDRENIENWLFSCKNDTCPVTKQPLNDTDLML 62

Query: 70  TPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKA 129
           TPNHTLRRLIQ WCTL+AS G+ERIPTPK P+D++ + K+L +AKN P S  KCL  LK+
Sbjct: 63  TPNHTLRRLIQVWCTLHASSGIERIPTPKPPVDESQIVKLLNEAKNVPGSQIKCLQRLKS 122

Query: 130 IALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEAHLKNLVNG 189
           IA + ERN   + +S GA E++A+++K  G   +  ++ AVEILFH+KSSE HLKNLV  
Sbjct: 123 IASKGERNKKYL-ESAGAIEYLASIVKKNGSTTV-ILDTAVEILFHLKSSETHLKNLVTN 182

Query: 190 DGNFINSLTFVLKN-GNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVRVIHDHIS 249
           +   I+SL  VL+  GN QSRAYA +++KS L+LADP++L++++ EL  E+V V+ D IS
Sbjct: 183 ESEIIDSLVMVLRTCGNYQSRAYATVVMKSILDLADPVQLITVKTELFVEVVNVLRDQIS 242

Query: 250 HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVILDQLCGCA 309
            QASK+ALK+L EVCPWGRNR+KAV+GGAV VL+ELLL ++E+R+ E+ LV+LDQLCGCA
Sbjct: 243 QQASKAALKLLLEVCPWGRNRIKAVQGGAVSVLIELLLGTSEKRFCELALVVLDQLCGCA 302

Query: 310 EGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVAKL 369
           EGR +L++H AG+A+VSKKILRVS +AS+RAVRILSS+CRFSA+ +VV EML VGVVAKL
Sbjct: 303 EGRAELLKHGAGIAVVSKKILRVSHVASERAVRILSSVCRFSATPRVVHEMLSVGVVAKL 362

Query: 370 CLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 412
           CLVLQ++   K +E+A++ILKLHSRVW +S+CIP HL S+YPSS
Sbjct: 363 CLVLQVNSGLKAKERAKEILKLHSRVWKSSNCIPVHLLSSYPSS 404

BLAST of Cp4.1LG16g08260 vs. TrEMBL
Match: A0A0D2NQL3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G116400 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.8e-140
Identity = 256/414 (61.84%), Postives = 324/414 (78.26%), Query Frame = 1

Query: 10  DLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVT 69
           ++E+PS FLCPISLQLMRDPVTISTGITYDR +IE+WLFSC  + CP+TKQ L D DL+ 
Sbjct: 3   EIEIPSLFLCPISLQLMRDPVTISTGITYDRDSIEQWLFSCKNKVCPVTKQVLHDSDLI- 62

Query: 70  PNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKAI 129
           PNHTLRRLIQ+WCT+NASHGVERIPTPK PIDKT +AK+LK AK FP    KCL  L++I
Sbjct: 63  PNHTLRRLIQSWCTVNASHGVERIPTPKPPIDKTQIAKLLKDAKKFPEMQVKCLKRLRSI 122

Query: 130 ALESERNCTLVAQSDGAFEFVAAVIKS------------GGDKNIGSIELAVEILFHIKS 189
            LE ERN + + ++ GA EF+ ++IK+             G + + + + A+ IL+HIK 
Sbjct: 123 TLEGERNRSCL-EAAGAVEFLVSIIKTYNSTLLLETESNEGPEFLKASDEALSILYHIKV 182

Query: 190 SEAHLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSE 249
           SE+ LK++++ D  F+ SL  +L+NG+ QSRAYA MLLK   E+ADPI L+S+  +  +E
Sbjct: 183 SESCLKSIISNDYEFVESLVQILRNGSYQSRAYATMLLKDIFEVADPIHLISLTPDFFTE 242

Query: 250 IVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGL 309
           IV  + D IS QASK+ALK+L E+CPWGRNR+KAVEGGAV VL+ELLL S+++R SE+ +
Sbjct: 243 IVHTLRDQISQQASKAALKLLVELCPWGRNRIKAVEGGAVFVLIELLLESSDKRASELAM 302

Query: 310 VILDQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQE 369
           V+LDQLCGCAEGR + + H AG+A+VSKKI RVS + S RAVRILSSICRFSA+ +V+QE
Sbjct: 303 VVLDQLCGCAEGRAEFLNHGAGLAMVSKKIFRVSHVVSGRAVRILSSICRFSATSRVLQE 362

Query: 370 MLEVGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 412
           ML+VGVVAKLC VLQ+D S KT+EKAR++LKLHSRVW N SCIP HL S+YPSS
Sbjct: 363 MLQVGVVAKLCFVLQLDSSYKTKEKAREMLKLHSRVWRNHSCIPSHLLSSYPSS 414

BLAST of Cp4.1LG16g08260 vs. TrEMBL
Match: M5W6C1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023672mg PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 3.9e-140
Identity = 255/406 (62.81%), Postives = 323/406 (79.56%), Query Frame = 1

Query: 10  DLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVT 69
           ++++PS+FLCPISL++MRDPVTISTGITYDR +IE+WLFS   + CP+TKQPL D   +T
Sbjct: 3   EVDIPSHFLCPISLEIMRDPVTISTGITYDRESIERWLFSGKNKACPVTKQPLFDGTDLT 62

Query: 70  PNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKAI 129
           PNHTLRR+IQAWCTLNAS GVERIPTPKSP+DK+ + K+++QAK  P++N  CL  L++I
Sbjct: 63  PNHTLRRIIQAWCTLNASQGVERIPTPKSPLDKSQIVKLIEQAKKLPNTNLHCLRRLRSI 122

Query: 130 ALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEAHLKNLVNGD 189
           A+          ++ GA E+VA++I      +   IE+AVE+LF +K SEA LK+L++ D
Sbjct: 123 AITQSERTKSCLEAAGAVEYVASIINKAPSDDPSIIEVAVEVLFLLKGSEAGLKDLISND 182

Query: 190 G--NFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVRVIHDHIS 249
           G  N + SL   LK  N QSRA+AI+LLKS  ++ADPI+LMS + E   EI+ V+ D IS
Sbjct: 183 GDNNIVESLVKALKFKNFQSRAHAIILLKSVFKVADPIQLMSAKVECFHEILHVLDDRIS 242

Query: 250 HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVILDQLCGCA 309
            QASK+ALK+L E+CPWGRNR+KAVEGGAV VL+E LL + E+R SE+ L++LDQLCGCA
Sbjct: 243 QQASKAALKLLVELCPWGRNRIKAVEGGAVAVLIEALLETHEKRASELILILLDQLCGCA 302

Query: 310 EGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVAKL 369
           EGR +L++H+AG+AIVSKKILRVS +ASDRAVRILSSICRFSA+  V+QEML+VGVVAKL
Sbjct: 303 EGRAELLKHAAGLAIVSKKILRVSHVASDRAVRILSSICRFSATCGVLQEMLQVGVVAKL 362

Query: 370 CLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSSPS 414
           CLVLQ+DC  KT+EKAR++LKLHSRVW NSSCIP HL   YPSS S
Sbjct: 363 CLVLQVDCRFKTKEKAREVLKLHSRVWKNSSCIPGHLLPFYPSSSS 408

BLAST of Cp4.1LG16g08260 vs. TrEMBL
Match: A0A061GUX0_THECC (U-box domain-containing protein 23 OS=Theobroma cacao GN=TCM_041222 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.4e-137
Identity = 254/413 (61.50%), Postives = 321/413 (77.72%), Query Frame = 1

Query: 10  DLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVT 69
           ++E+P +FLCPISLQLMRDPVTISTGITYDR +IEKWLFSC   TCP++KQ L D DL T
Sbjct: 3   EIEIPPHFLCPISLQLMRDPVTISTGITYDRDSIEKWLFSCKNNTCPVSKQALHDSDL-T 62

Query: 70  PNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKAI 129
           PNHTLRRLIQAWC LNASHG+ERIPTPK  +DKT ++K+LK AK FP    KCL  L++I
Sbjct: 63  PNHTLRRLIQAWCILNASHGIERIPTPKPLVDKTQISKLLKDAKKFPEMQLKCLKRLRSI 122

Query: 130 ALESERNCTLVAQSDGAFEFVAAVIKSG-----------GDKNIGSIELAVEILFHIKSS 189
            LE ERN + + ++ GA +F+ ++IKS            G + + + + A+ IL+HIK S
Sbjct: 123 TLEGERNRSYL-EAAGAVQFLVSMIKSNDSTLLEVESNEGSELLKASDEALSILYHIKVS 182

Query: 190 EAHLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEI 249
           ++ LK++++ D  F+ SL  V+K GN QSRAYA MLLK   E+ADPI+LMS      +E+
Sbjct: 183 QSCLKSIISNDQEFVESLVQVMKYGNYQSRAYATMLLKDVFEVADPIQLMSATPVFFAEM 242

Query: 250 VRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLV 309
           VR + D IS QASK++LK+L E+CPWGRNR+KAVEGGAV VL+E LL ++E+R  E+ L+
Sbjct: 243 VRTLRDQISQQASKASLKLLVELCPWGRNRIKAVEGGAVFVLIEFLLKTSEKRACELALI 302

Query: 310 ILDQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEM 369
           +LDQLC CAEGR +L++H AG+AIVSKKI RVS +ASDRAVRILSSICR SA+  V+QEM
Sbjct: 303 LLDQLCACAEGRAELLKHGAGLAIVSKKIFRVSHVASDRAVRILSSICRCSATSWVLQEM 362

Query: 370 LEVGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 412
           L+VGVV+KLCLVLQ+D S KT+E+AR+ILKLHSRVW N SCIP HL S+YPSS
Sbjct: 363 LQVGVVSKLCLVLQLDSSYKTKERAREILKLHSRVWRNPSCIPSHLFSSYPSS 413

BLAST of Cp4.1LG16g08260 vs. TAIR10
Match: AT2G35930.1 (AT2G35930.1 plant U-box 23)

HSP 1 Score: 418.7 bits (1075), Expect = 4.2e-117
Identity = 205/406 (50.49%), Postives = 304/406 (74.88%), Query Frame = 1

Query: 10  DLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVT 69
           ++E+P +FLCPISL++M+DPV +STGITYDR +IEKWLF+  K +CP+TKQ ++D DL T
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADL-T 68

Query: 70  PNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKAI 129
           PNHTLRRLIQ+WCTLNAS+GVERIPTP+ PI K+ + K+++ + +   +  KCL  L+ I
Sbjct: 69  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 128

Query: 130 ALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSI-ELAVEILFHIKSSEAHLKNLVNG 189
             E+  N   + ++ G  EF+A ++ +  D   GS+ + A+ +L+H+++SE  LKNL+N 
Sbjct: 129 VSENATNKRCL-EAAGVPEFLANIVSN--DSENGSLTDEALNLLYHLETSETVLKNLLNN 188

Query: 190 --DGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVRVIHDHI 249
             D N + SLT +++ G  +SR YA +LLK+ LE+ADP++ M+++ E+ +E+V+++ D I
Sbjct: 189 KKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRI 248

Query: 250 SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLN---STERRWSEIGLVILDQL 309
           S +A+K+A+ +L  +CPWGRNR KAVE G + V++ELL++   ++ERR  E+ +V+LD L
Sbjct: 249 SQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLL 308

Query: 310 CGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGV 369
           C CAEGR + + H A +A+V KKILRVS  ASDRAVR+L S+ RF A+  ++ EML++GV
Sbjct: 309 CQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGV 368

Query: 370 VAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYP 410
           VAKLCLVLQ+ C  KT+EKA+++LKLH+RVW +S C+P ++  AYP
Sbjct: 369 VAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of Cp4.1LG16g08260 vs. TAIR10
Match: AT3G52450.1 (AT3G52450.1 plant U-box 22)

HSP 1 Score: 417.2 bits (1071), Expect = 1.2e-116
Identity = 207/433 (47.81%), Postives = 307/433 (70.90%), Query Frame = 1

Query: 10  DLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVT 69
           ++E+PS+FLCPISL +M+DPV +STGITYDR +IEKWLFS  K +CP+TKQ +++ DL T
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDL-T 63

Query: 70  PNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKAI 129
           PNHTLRRLIQ+WCTLNAS+G+ERIPTPK PI K+ + K++K++ +   +  KCL  L+ I
Sbjct: 64  PNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQI 123

Query: 130 ALESERN-------------CTLVAQSDGAFEFVAAVIKSGG--------------DKNI 189
             E+  N               +V+ S   +   ++ + S                D + 
Sbjct: 124 VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 183

Query: 190 GSIELAVEILFHIKSSEAHLKNLVNG--DGNFINSLTFVLKNGNCQSRAYAIMLLKSSLE 249
             ++ A+ +L+H+ +SE  LK+L+N     N + +LT +++ G  +SRAYA +LLK  LE
Sbjct: 184 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLE 243

Query: 250 LADPIRLMSIERELLSEIVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVL 309
           +ADP++++ +EREL  E+++++HD ISH+A++SA+++L   CPWGRNR KAVEGG + ++
Sbjct: 244 VADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMI 303

Query: 310 VELLLN---STERRWSEIGLVILDQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDR 369
           +ELL++   S+ERR SE+ +V+LD LC CAEGR + + H A +A+VSKKILRVS + S+R
Sbjct: 304 IELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSER 363

Query: 370 AVRILSSICRFSASVKVVQEMLEVGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNS 411
           AVR+L S+ RF A+  ++QEML++GVVAKLCLVLQ+ C +KT+EKA+++LKLH+RVW  S
Sbjct: 364 AVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHARVWRES 423

BLAST of Cp4.1LG16g08260 vs. TAIR10
Match: AT3G19380.1 (AT3G19380.1 plant U-box 25)

HSP 1 Score: 204.9 bits (520), Expect = 9.5e-53
Identity = 132/394 (33.50%), Postives = 210/394 (53.30%), Query Frame = 1

Query: 11  LEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVTP 70
           +++P +F CPISL+LM+DPVT+ TG TYDR++IE W+   N  TCP+T+ PLSD  L+ P
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLI-P 71

Query: 71  NHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPS------SNHKCLL 130
           NHTLRRLIQ WC  N S+GVERIPTPK P D T V  +L QA           S    L 
Sbjct: 72  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 131

Query: 131 ALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEAHLKN 190
            L+  A +S++N  L+A  +     +  +        + S  LA+ ++  I      +  
Sbjct: 132 RLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFVS- 191

Query: 191 LVNGDGNFINSLTFVLKNGNCQSRAYA---IMLLKSSLELADPIRLMSIERELLSEIVRV 250
            ++ D   +  LT +L + + ++R  A   I ++ +  + AD    +S    +   ++ +
Sbjct: 192 -ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLDL 251

Query: 251 IHDHI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVIL 310
           + + I S +A K  +K L  +C     R  A+  GA  +L++ L    +R  +E  L  +
Sbjct: 252 LRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATV 311

Query: 311 DQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 370
           + LC   EG     EH+  V ++ K ILRVS  A++ A   L ++C  +A  +  +E   
Sbjct: 312 ELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC--TAEERWREEAAG 371

Query: 371 VGVVAKLCLVLQMDCSSKTREKARDILKLHSRVW 395
            GVV +L L++Q +C+ + ++KA+ +LKL    W
Sbjct: 372 AGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of Cp4.1LG16g08260 vs. TAIR10
Match: AT1G49780.1 (AT1G49780.1 plant U-box 26)

HSP 1 Score: 201.1 bits (510), Expect = 1.4e-51
Identity = 138/404 (34.16%), Postives = 215/404 (53.22%), Query Frame = 1

Query: 11  LEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVTP 70
           +++P +F CPISL LM DPVTISTG TYDR++I+ W+   N  TCP+T+  LSD  L+ P
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNT-TCPVTRVALSDFTLI-P 71

Query: 71  NHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPS------SNHKCLL 130
           NHTLRRLIQ WC  N S+GVERIPTPK P D   V  +L QA           S    + 
Sbjct: 72  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIR 131

Query: 131 ALKAIALESERNCTLVAQSDGAFEFV----AAVIKSGGDKNIGSIELAVEILFHIKSSEA 190
            L+ +A +SE+N  L+A  +     V    A +  +     + S  LA+ +L H+  +E 
Sbjct: 132 RLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVSESLALLVLLHMTETEC 191

Query: 191 HLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRL---MSIERELLSE 250
                V  D + +  +T +L + + + R  A  L++  L  A  + L   +S    +   
Sbjct: 192 ---EAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEG 251

Query: 251 IVRVIHDHI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIG 310
           ++ ++ + I S +A K  +K +  +C   + R  A+  GA G+L++ L    +R  +E G
Sbjct: 252 VLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTERG 311

Query: 311 LVILDQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQ 370
           L  ++ LC   EG     EH+  V ++ K ILRVS  A++ A   L ++C  +A  +   
Sbjct: 312 LATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC--TAEERCRD 371

Query: 371 EMLEVGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCI 401
           E    G+V +L L++Q DC+ + + KA+ +LKL    W + S +
Sbjct: 372 EAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTV 408

BLAST of Cp4.1LG16g08260 vs. TAIR10
Match: AT5G37490.1 (AT5G37490.1 ARM repeat superfamily protein)

HSP 1 Score: 164.5 bits (415), Expect = 1.4e-40
Identity = 116/407 (28.50%), Postives = 217/407 (53.32%), Query Frame = 1

Query: 4   EEDGGGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLS 63
           E +   ++ +P  F CPIS+ LM+DPV ISTGITYDR +IE W+ S NK TCP+T   L+
Sbjct: 22  ESEPESEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNK-TCPVTNTVLT 81

Query: 64  DPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFP--SSNHK 123
             D + PNHT+R++IQ WC    S  ++RIPTP+ P+    V +I ++  +        K
Sbjct: 82  TFDQI-PNHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEK 141

Query: 124 C---LLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSI--ELAVEILFHIK 183
           C   +  +K +  ESE+N   V ++   +       K  GD+ +  +  E+   + +   
Sbjct: 142 CGVIIEKIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKLTFMLNEILSLLTWMFP 201

Query: 184 SSEAHLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADP-IRLMSIERELL 243
                +  L +        +  +LK+ +   R  A  ++K  L L +  +   ++E  + 
Sbjct: 202 IGLEGISKLASATS--FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVA 261

Query: 244 SEIVRVIHDHISHQASKSALKVLAE-VCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSE 303
             +V++I D +S  ++KS+L  + + V        + +E G V + VE+++++ E    E
Sbjct: 262 EALVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDA-ENSVCE 321

Query: 304 IGLVILDQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKV 363
             L +LD +C    GRE++ +++  + ++ KKI +VS +A+  ++ ++  + +   +V  
Sbjct: 322 KALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTV-A 381

Query: 364 VQEMLEVGVVAKLCLVLQMDCSSKTREKARDILK-LHSRVWSNSSCI 401
           V++ + +G   K+ LVLQ+    +T+EKA ++LK +++++   S C+
Sbjct: 382 VEDAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCV 422

BLAST of Cp4.1LG16g08260 vs. NCBI nr
Match: gi|659097388|ref|XP_008449598.1| (PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis melo])

HSP 1 Score: 719.9 bits (1857), Expect = 2.5e-204
Identity = 365/411 (88.81%), Postives = 392/411 (95.38%), Query Frame = 1

Query: 2   RMEEDGGGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQP 61
           ++EE+GG  +EVPSYFLCPISLQLMRDPVTISTGITYDRS+IEKWLFS NK  CPITKQP
Sbjct: 6   QLEENGG--IEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQP 65

Query: 62  LS-DPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNH 121
           LS DPDL+TPNHTLRRLIQ+WCTLNAS+G+ERIPTPKSPIDKTH+AKILKQA+NFP+SNH
Sbjct: 66  LSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSNH 125

Query: 122 KCLLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEA 181
           KCLLALKAIALE+ERN  LVAQSDGAF+F+A VIKSGG  NI SIELAVEILFHIKSS+A
Sbjct: 126 KCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQA 185

Query: 182 HLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVR 241
           HLKNLVNGD +FINSLTFVL+NGNCQSRAYA+MLLKSSLELADPIRLMSIERELL E VR
Sbjct: 186 HLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETVR 245

Query: 242 VIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVIL 301
           VIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSE+GLV+L
Sbjct: 246 VIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVVL 305

Query: 302 DQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLE 361
           DQLCGCAEGREKL+EH+AG+A+VSKKILRVSAMASDRAVRILSSICRFSAS KV+QEMLE
Sbjct: 306 DQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEMLE 365

Query: 362 VGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 412
           VGVVAKLCLVLQMDCS KTREKARD LKLHSRVWSNSSCIPPHL SAYPS+
Sbjct: 366 VGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 414

BLAST of Cp4.1LG16g08260 vs. NCBI nr
Match: gi|778716759|ref|XP_004140266.2| (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis sativus])

HSP 1 Score: 712.2 bits (1837), Expect = 5.2e-202
Identity = 362/414 (87.44%), Postives = 389/414 (93.96%), Query Frame = 1

Query: 1   MRMEE--DGGGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPIT 60
           M+ME+  +  G++EVPSYFLCPISLQLMRDPVTISTGITYDRS+IE WLFS NK  CPIT
Sbjct: 1   MKMEDHLEENGEIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEMWLFSFNKTVCPIT 60

Query: 61  KQPLS-DPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPS 120
           KQPLS DPDL+TPNHTLRRLIQ+WCTLNAS+G+ERIPTPKSPIDKTH+AKILKQA++FP 
Sbjct: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQSFPD 120

Query: 121 SNHKCLLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKS 180
           S HKCLLALKAIALE+ERN  LVAQSDGAF+F+A VIKSGG  NI SIELAVEILFHIKS
Sbjct: 121 STHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180

Query: 181 SEAHLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSE 240
           S+ HLKNLVNGD +FINSLTFVL+NGNCQSRAYA+MLLKSSLELADPIRLMSIERELL E
Sbjct: 181 SQTHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240

Query: 241 IVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGL 300
           IVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSE+GL
Sbjct: 241 IVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300

Query: 301 VILDQLCGCAEGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQE 360
           VILDQLCGCAEGREKL+ H+AG+A+VSKKILRVSAMASDRAVRILSSICRFSAS KV+QE
Sbjct: 301 VILDQLCGCAEGREKLVAHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVMQE 360

Query: 361 MLEVGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 412
           MLEVGVVAKLCLVLQMDCS KTREKARD LKLHSRVWSNSSCIPPHL SAYPS+
Sbjct: 361 MLEVGVVAKLCLVLQMDCSMKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 414

BLAST of Cp4.1LG16g08260 vs. NCBI nr
Match: gi|700192847|gb|KGN48051.1| (hypothetical protein Csa_6G426370 [Cucumis sativus])

HSP 1 Score: 709.1 bits (1829), Expect = 4.4e-201
Identity = 359/404 (88.86%), Postives = 383/404 (94.80%), Query Frame = 1

Query: 9   GDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLS-DPDL 68
           G++EVPSYFLCPISLQLMRDPVTISTGITYDRS+IE WLFS NK  CPITKQPLS DPDL
Sbjct: 9   GEIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEMWLFSFNKTVCPITKQPLSSDPDL 68

Query: 69  VTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALK 128
           +TPNHTLRRLIQ+WCTLNAS+G+ERIPTPKSPIDKTH+AKILKQA++FP S HKCLLALK
Sbjct: 69  LTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQSFPDSTHKCLLALK 128

Query: 129 AIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEAHLKNLVN 188
           AIALE+ERN  LVAQSDGAF+F+A VIKSGG  NI SIELAVEILFHIKSS+ HLKNLVN
Sbjct: 129 AIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQTHLKNLVN 188

Query: 189 GDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVRVIHDHIS 248
           GD +FINSLTFVL+NGNCQSRAYA+MLLKSSLELADPIRLMSIERELL EIVRVIHDHIS
Sbjct: 189 GDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLREIVRVIHDHIS 248

Query: 249 HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVILDQLCGCA 308
           HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSE+GLVILDQLCGCA
Sbjct: 249 HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVILDQLCGCA 308

Query: 309 EGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVAKL 368
           EGREKL+ H+AG+A+VSKKILRVSAMASDRAVRILSSICRFSAS KV+QEMLEVGVVAKL
Sbjct: 309 EGREKLVAHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVMQEMLEVGVVAKL 368

Query: 369 CLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 412
           CLVLQMDCS KTREKARD LKLHSRVWSNSSCIPPHL SAYPS+
Sbjct: 369 CLVLQMDCSMKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 412

BLAST of Cp4.1LG16g08260 vs. NCBI nr
Match: gi|703077099|ref|XP_010090504.1| (E3 ubiquitin-protein ligase [Morus notabilis])

HSP 1 Score: 518.5 bits (1334), Expect = 1.1e-143
Identity = 256/404 (63.37%), Postives = 329/404 (81.44%), Query Frame = 1

Query: 10  DLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLV- 69
           +++VP  FLCPISLQLMRDPVT+STGITYDR NIE WLFSC   TCP+TKQPL+D DL+ 
Sbjct: 3   EIDVPYLFLCPISLQLMRDPVTVSTGITYDRENIENWLFSCKNDTCPVTKQPLNDTDLML 62

Query: 70  TPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKA 129
           TPNHTLRRLIQ WCTL+AS G+ERIPTPK P+D++ + K+L +AKN P S  KCL  LK+
Sbjct: 63  TPNHTLRRLIQVWCTLHASSGIERIPTPKPPVDESQIVKLLNEAKNVPGSQIKCLQRLKS 122

Query: 130 IALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEAHLKNLVNG 189
           IA + ERN   + +S GA E++A+++K  G   +  ++ AVEILFH+KSSE HLKNLV  
Sbjct: 123 IASKGERNKKYL-ESAGAIEYLASIVKKNGSTTV-ILDTAVEILFHLKSSETHLKNLVTN 182

Query: 190 DGNFINSLTFVLKN-GNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVRVIHDHIS 249
           +   I+SL  VL+  GN QSRAYA +++KS L+LADP++L++++ EL  E+V V+ D IS
Sbjct: 183 ESEIIDSLVMVLRTCGNYQSRAYATVVMKSILDLADPVQLITVKTELFVEVVNVLRDQIS 242

Query: 250 HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVILDQLCGCA 309
            QASK+ALK+L EVCPWGRNR+KAV+GGAV VL+ELLL ++E+R+ E+ LV+LDQLCGCA
Sbjct: 243 QQASKAALKLLLEVCPWGRNRIKAVQGGAVSVLIELLLGTSEKRFCELALVVLDQLCGCA 302

Query: 310 EGREKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVAKL 369
           EGR +L++H AG+A+VSKKILRVS +AS+RAVRILSS+CRFSA+ +VV EML VGVVAKL
Sbjct: 303 EGRAELLKHGAGIAVVSKKILRVSHVASERAVRILSSVCRFSATPRVVHEMLSVGVVAKL 362

Query: 370 CLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 412
           CLVLQ++   K +E+A++ILKLHSRVW +S+CIP HL S+YPSS
Sbjct: 363 CLVLQVNSGLKAKERAKEILKLHSRVWKSSNCIPVHLLSSYPSS 404

BLAST of Cp4.1LG16g08260 vs. NCBI nr
Match: gi|1009113451|ref|XP_015873152.1| (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Ziziphus jujuba])

HSP 1 Score: 511.9 bits (1317), Expect = 1.0e-141
Identity = 256/401 (63.84%), Postives = 327/401 (81.55%), Query Frame = 1

Query: 11  LEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQPLSDPDLVTP 70
           ++VPS+FLCPISLQLMRDPV +STGITYDR +IEKWLFSC K TCP+TKQPL D DL TP
Sbjct: 12  VDVPSHFLCPISLQLMRDPVIVSTGITYDRESIEKWLFSCKKTTCPVTKQPLFDSDL-TP 71

Query: 71  NHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPSSNHKCLLALKAIA 130
           NHTLRRLIQAWCTLNAS+G+ERIPTPKSPIDK  + K+L +A N+PS + +CLL L++IA
Sbjct: 72  NHTLRRLIQAWCTLNASNGIERIPTPKSPIDKAQIVKLLNEANNYPSRHVQCLLRLRSIA 131

Query: 131 LESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSIELAVEILFHIKSSEAHLKNLVNGDG 190
            +SERN  ++ ++ GA EF+ ++IK   + N    E A+E+LFH+K+SEA  + ++  D 
Sbjct: 132 SQSERNKNILEEA-GAIEFLVSLIKKKKNDNSSISETAIEVLFHLKNSEARFRQVMKEDS 191

Query: 191 NFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELLSEIVRVIHDHISHQA 250
             I+SL  VL+ GN +SRA+A +LLKS LE+ADPI+L+S+E EL  E+V V+ D IS  A
Sbjct: 192 EIIDSLVRVLRCGNYKSRAHAAVLLKSLLEVADPIQLVSLETELFREVVNVVRDQISQAA 251

Query: 251 SKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSEIGLVILDQLCGCAEGR 310
           SK+ALK+L E+ PWGRNR+KAVE GAV +L+ELLL + E+R  E+ L  LDQLCGCAEGR
Sbjct: 252 SKAALKLLVEISPWGRNRIKAVEAGAVLILIELLLETFEKRACELVLTALDQLCGCAEGR 311

Query: 311 EKLMEHSAGVAIVSKKILRVSAMASDRAVRILSSICRFSASVKVVQEMLEVGVVAKLCLV 370
            +L+ HSAG+A+VSKKILRVS +AS++AVRILSSI RFSA+ +V+QEML+VGVV KLCLV
Sbjct: 312 AELLMHSAGLAVVSKKILRVSHVASEKAVRILSSISRFSATSRVLQEMLQVGVVGKLCLV 371

Query: 371 LQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 412
           +Q+D S KT++KA++ILKLHSRVW NS+CIPPHL S+YPSS
Sbjct: 372 IQVDSSLKTKDKAKEILKLHSRVWKNSTCIPPHLLSSYPSS 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PUB23_ARATH7.4e-11650.49E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana GN=PUB23 PE=1 SV=1[more]
PUB22_ARATH2.2e-11547.81E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana GN=PUB22 PE=1 SV=1[more]
PUB25_ARATH1.7e-5133.50U-box domain-containing protein 25 OS=Arabidopsis thaliana GN=PUB25 PE=2 SV=1[more]
PUB26_ARATH2.4e-5034.16U-box domain-containing protein 26 OS=Arabidopsis thaliana GN=PUB26 PE=2 SV=1[more]
PUB21_ARATH2.5e-3928.50U-box domain-containing protein 21 OS=Arabidopsis thaliana GN=PUB21 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KJY2_CUCSA3.1e-20188.86Uncharacterized protein OS=Cucumis sativus GN=Csa_6G426370 PE=4 SV=1[more]
W9QTE7_9ROSA7.7e-14463.37E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_017126 PE=4 SV=1[more]
A0A0D2NQL3_GOSRA1.8e-14061.84Uncharacterized protein OS=Gossypium raimondii GN=B456_006G116400 PE=4 SV=1[more]
M5W6C1_PRUPE3.9e-14062.81Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023672mg PE=4 SV=1[more]
A0A061GUX0_THECC1.4e-13761.50U-box domain-containing protein 23 OS=Theobroma cacao GN=TCM_041222 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G35930.14.2e-11750.49 plant U-box 23[more]
AT3G52450.11.2e-11647.81 plant U-box 22[more]
AT3G19380.19.5e-5333.50 plant U-box 25[more]
AT1G49780.11.4e-5134.16 plant U-box 26[more]
AT5G37490.11.4e-4028.50 ARM repeat superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659097388|ref|XP_008449598.1|2.5e-20488.81PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis melo][more]
gi|778716759|ref|XP_004140266.2|5.2e-20287.44PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis sativus][more]
gi|700192847|gb|KGN48051.1|4.4e-20188.86hypothetical protein Csa_6G426370 [Cucumis sativus][more]
gi|703077099|ref|XP_010090504.1|1.1e-14363.37E3 ubiquitin-protein ligase [Morus notabilis][more]
gi|1009113451|ref|XP_015873152.1|1.0e-14163.84PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005488binding
GO:0004842ubiquitin-protein transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
Vocabulary: INTERPRO
TermDefinition
IPR016024ARM-type_fold
IPR013083Znf_RING/FYVE/PHD
IPR011989ARM-like
IPR003613Ubox_domain
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0005488 binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g08260.1Cp4.1LG16g08260.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainPFAMPF04564U-boxcoord: 13..83
score: 9.0
IPR003613U box domainSMARTSM00504Ubox_2coord: 16..81
score: 2.5
IPR003613U box domainPROFILEPS51698U_BOXcoord: 12..88
score: 38
IPR011989Armadillo-like helicalGENE3DG3DSA:1.25.10.10coord: 77..388
score: 1.2
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 12..76
score: 6.5
IPR016024Armadillo-type foldunknownSSF48371ARM repeatcoord: 184..389
score: 1.32
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 10..410
score: 3.6E
NoneNo IPR availablePANTHERPTHR22849:SF11SUBFAMILY NOT NAMEDcoord: 10..410
score: 3.6E
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 8..87
score: 5.55

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG16g08260Cucsa.241890Cucumber (Gy14) v1cgycpeB0640
Cp4.1LG16g08260Cucsa.185910Cucumber (Gy14) v1cgycpeB0508
Cp4.1LG16g08260CmaCh06G005270Cucurbita maxima (Rimu)cmacpeB820
Cp4.1LG16g08260CmaCh14G003620Cucurbita maxima (Rimu)cmacpeB264
Cp4.1LG16g08260CmaCh20G001460Cucurbita maxima (Rimu)cmacpeB564
Cp4.1LG16g08260CmoCh20G001630Cucurbita moschata (Rifu)cmocpeB517
Cp4.1LG16g08260CmoCh06G005260Cucurbita moschata (Rifu)cmocpeB774
Cp4.1LG16g08260Cla017623Watermelon (97103) v1cpewmB277
Cp4.1LG16g08260Cla008618Watermelon (97103) v1cpewmB287
Cp4.1LG16g08260Csa3G782630Cucumber (Chinese Long) v2cpecuB273
Cp4.1LG16g08260Csa6G426370Cucumber (Chinese Long) v2cpecuB281
Cp4.1LG16g08260MELO3C009543Melon (DHL92) v3.5.1cpemeB277
Cp4.1LG16g08260MELO3C014520Melon (DHL92) v3.5.1cpemeB278
Cp4.1LG16g08260ClCG10G018310Watermelon (Charleston Gray)cpewcgB250
Cp4.1LG16g08260ClCG02G022440Watermelon (Charleston Gray)cpewcgB255
Cp4.1LG16g08260CSPI06G23430Wild cucumber (PI 183967)cpecpiB283
Cp4.1LG16g08260CSPI03G35650Wild cucumber (PI 183967)cpecpiB276
Cp4.1LG16g08260Lsi10G011360Bottle gourd (USVL1VR-Ls)cpelsiB227
Cp4.1LG16g08260Lsi03G018160Bottle gourd (USVL1VR-Ls)cpelsiB243
Cp4.1LG16g08260MELO3C009543.2Melon (DHL92) v3.6.1cpemedB323
Cp4.1LG16g08260MELO3C014520.2Melon (DHL92) v3.6.1cpemedB324
Cp4.1LG16g08260CsaV3_6G038570Cucumber (Chinese Long) v3cpecucB0348
Cp4.1LG16g08260Bhi10G000148Wax gourdcpewgoB0354
Cp4.1LG16g08260CsGy6G022720Cucumber (Gy14) v2cgybcpeB755
Cp4.1LG16g08260CsGy3G033130Cucumber (Gy14) v2cgybcpeB346
Cp4.1LG16g08260Carg18410Silver-seed gourdcarcpeB0579
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG16g08260Cp4.1LG08g09610Cucurbita pepo (Zucchini)cpecpeB326
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG16g08260Cucurbita pepo (Zucchini)cpecpeB302
Cp4.1LG16g08260Cucurbita pepo (Zucchini)cpecpeB310
Cp4.1LG16g08260Cucurbita maxima (Rimu)cmacpeB627
Cp4.1LG16g08260Cucurbita moschata (Rifu)cmocpeB228
Cp4.1LG16g08260Cucurbita moschata (Rifu)cmocpeB576
Cp4.1LG16g08260Silver-seed gourdcarcpeB0350
Cp4.1LG16g08260Silver-seed gourdcarcpeB1273