Cp4.1LG16g04140 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG16g04140
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	O-methyltransferase
Location	Cp4.1LG16 : 5572830 .. 5576326 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGGTAATGTTCTTAAATTAAGAAATTAGATATGTTTAAAAAACATTAAATTTTTTAATGGGTTTTTGATAAACAGTGGATATTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCTGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAGGACAGGGAGTCCACTGAAACTCAACTGTTCTTTGATGTTTTGATGATGTCCGATCTAAACGGCATGGAGAGAAACGAGAGAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGGTCACTTATTGAGGTGTACCCTTAACTCAAAAGTGTTGTTATTTGCTGCTTAATTANGCTACAATTGCTTCTTTGGGAGCTTTGAGCGGTTGGCTTTGTAGCTCAGATCAAGACCATAACTCCAGTTCCTTTGAAATGGCAAACGGGAAGTCCATGTGGGATTACTCTGCTCAGGAACCAAGTTTTGGCAATTTCTTTCATCAGGCAATGGTTTGCGATTCTGAATTGCTTGGTAGAATAGTGATTGAGAAGTGTAGCGGAGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCAGCAACGAACTGCAAAAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGGTAATGTTCTTAAATTAAGAAATTAGATATGTTTAAAAAACATTAAATTTTTTAATGGGTTTTTGATAAACAGTGGATATTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCTGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAGGACAGGGAGTCCACTGAAACTCAACTGTTCTTTGATGTTTTGATGATGTCCGATCTAAACGGCATGGAGAGAAACGAGAGAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGGTCACTTATTGAGGTGTACCCTTAACTCAAAAGTGTTGTTATTTGCTGCTTAATTATGCTTCTTCTTGTTGTGGGTTCGTAGAACTTGTGGTGTTGGTTTCAGAAAACCAGAAACAAACCATAATAAAGGCTTCTATAATAAAGTTGTTATATTACTGATAGAAAATATAAGACTACCTATTACGCAACCTACTTGTAGTCTCTTTTTGCATCCTTATCTTAGTAGGTATCTACAACCTTGTCTCGGGGTTCTAGTGAGTGTGAACTAAGTTGCAACTCTCTTTTCTACCACTCAAGTGGCTTTTCTATCTTATGCATTGGATGATTAGTGTCTTGGGATGAGGCGCGACCTCACAAGTAAAACTGAGGGGACAGAATAGCTAGATCTGAATTAGTATCGCTCACTGAACATCATCAATGGAGGTTAGTGGACAACCATCCCATGAGACCATACTACCACTAAAATCGGGGGGTTGTGATCTCCTGTGTGCTTGTCGGCTACTAGATCGTCCTACGTGATCCCACGCTCTCCCACTCAAGGATGAGTTTCTACAGCTTAGGGGTCTTGTTCAGGAGACCCCTTAGCTGTCACCACTAGGTAGGGGCACAAATCATACTACCTTGTCCGACTCTACCTGGGTCAATGATCTCGGTATTGGAGTACTGTCTCGACGGTACGATATCGGAGCAGAGAGGGAAGTGGAGGTTCTTGTAACACTATCCACACTACTCAAGTTCGGCTAGCTACCTCAGTTGTCTATAGTTACCGTGGTGTCTTACTAGGTCTTTCTGGGGCGGATCTTGGTGTCTTAGACTTGGATTCATTCATCTATAGAATTTCCATGCAAGGTCTCTCAAACCTATCGTGGTCTAGAATATGGTCTGCCTCCGCTATTCATCCATTCGCGGGCCTGAATGCATAGTATTCTAGAGATATCCCATTCTAGGTCTTTGTCGAGCCTACTTAGTCTCAAGTGCCCTAGTCTAGGCTCGTCGTTTCTTCCTATGCTTAACAAGAAAGACAACACAAGCAATCATGCACTTAAGTTAACATGCAACAATTCATGCAATATTAACAAACAAGCTCGATGAGAACATAACGTTCATCTTTACCCAGAGCGCAGATTTTGCAAACTTCCATTCCTCCTGTATCATACATACATTATCTCAATCTCATTCTAAAGCAAATTCATAAGTCAATCAATTCTAGTTACCATTGTTTTTCTTATCTAGTTCTTTTCTACCGCTTATATTTTAATATGCTAGGTCCAGCTAGCTAGGATTTATTAGTTTGACAACGCATACTTATTCCAAGCGGTTCCTACAAGTACTATTCTTATAATCTAAAAGTTTTGTCGTTGAAGAGAGATTAGGACGGTGGATGAGAGTTTGCTTATTTAAACAATACTTTAATTTGCCCAATTTAATAACCATTCCCCTTTAAGCATGGTTCCAAACATGAGCTTGTGATGATGGATTCTGCAAGTTGTGAGGAAATTAATATCATAAATACAGCTTCCGGAGTTTGCCTGATTTCAGATCTGACCCCTAACAAAATCTAGATGGAAGAGAAGTTGGATGAGCTGCTTCAATCCCAAGCTCATGTTTGGAACCATGCTTTGAAATTTATGAACTCCATGGCTCTTAAATGTGCTGTTGAATTGGGCATACCAGATGTTATCCATAGCCACGCCCAACCCATGAGCCTCTCTGATTCAACCTTCAAAGGCTCAACACCTTGGCCGCCTCATGCGCCTCCTCGTCCATTCTGGCTTCTTTGATGCCAACGGGCAAGAGGACGTGAAATACAGCCTCACGCCCTCCTCTCGCCTTCTCCTCCGCCACACACACACCTACACCACTTTCCAAATGATACCCTTCTTGTGTCTTGCTTTGGATAAAGCTACAATTGCTTCTTTGGGAGCTTTGAGCGGTTGGCTTTGTAACTCAGATCAAGACCATAACTCCAGTTCCTTTGAAATGGCAAATGGGAAGCCCATATGGGAGTATGCTGCTCAGGAACCAAATTTTGGCAATTTGTTTCAACAGATAATGGTTTGTGATTCTGAATTGATTGGTAAAATAGTGATTGAGAAGTGTAGCAGAGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGGACATCACTTGCATTGTATTTGATCTCCCACAAGTGGTTTCCAACCAGCAACGAACTGCAAAAAACTTGAGCTTGATTACAACA

mRNA sequence

GAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGTGGATATTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCTGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAGGACAGGGAGTCCACTGAAACTCAACTGTTCTTTGATGTTTTGATGATGTCCGATCTAAACGGCATGGAGAGAAACGAGAGAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGCGGTTGGCTTTGTAGCTCAGATCAAGACCATAACTCCAGTTCCTTTGAAATGGCAAACGGGAAGTCCATGTGGGATTACTCTGCTCAGGAACCAAGTTTTGGCAATTTCTTTCATCAGGCAATGGTTTGCGATTCTGAATTGCTTGGTAGAATAGTGATTGAGAAGTGTAGCGGAGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCAGCAACGAACTGCAAAAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGTGGATATTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCTGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAGGACAGGGAGTCCACTGAAACTCAACTGTTCTTTGATGTTTTGATGATGTCCGATCTAAACGGCATGGAGAGAAACGAGAGAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGGTCACTTATTGAGATGGAAGAGAAGTTGGATGAGCTGCTTCAATCCCAAGCTCATGTTTGGAACCATGCTTTGAAATTTATGAACTCCATGGCTCTTAAATGTGCTGTTGAATTGGGCATACCAGATGCTCAACACCTTGGCCGCCTCATGCGCCTCCTCGTCCATTCTGGCTTCTTTGATGCCAACGGGCAAGAGGACGTGAAATACAGCCTCACGCCCTCCTCTCGCCTTCTCCTCCGCCACACACACACCTACACCACTTTCCAAATGATACCCTTCTTGTGTCTTGCTTTGGATAAAGCTACAATTGCTTCTTTGGGAGCTTTGAGCGGTTGGCTTTGTAACTCAGATCAAGACCATAACTCCAGTTCCTTTGAAATGGCAAATGGGAAGCCCATATGGGAGTATGCTGCTCAGGAACCAAATTTTGGCAATTTGTTTCAACAGATAATGGTTTGTGATTCTGAATTGATTGGTAAAATAGTGATTGAGAAGTGTAGCAGAGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGGACATCACTTGCATTGTATTTGATCTCCCACAAGTGGTTTCCAACCAGCAACGAACTGCAAAAAACTTGAGCTTGATTACAACA

Coding sequence (CDS)

ATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGTGGATATTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCTGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAGGACAGGGAGTCCACTGAAACTCAACTGTTCTTTGATGTTTTGATGATGTCCGATCTAAACGGCATGGAGAGAAACGAGAGAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGCGGTTGGCTTTGTAGCTCAGATCAAGACCATAACTCCAGTTCCTTTGAAATGGCAAACGGGAAGTCCATGTGGGATTACTCTGCTCAGGAACCAAGTTTTGGCAATTTCTTTCATCAGGCAATGGTTTGCGATTCTGAATTGCTTGGTAGAATAGTGATTGAGAAGTGTAGCGGAGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCAGCAACGAACTGCAAAAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGTGGATATTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCTGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAGGACAGGGAGTCCACTGAAACTCAACTGTTCTTTGATGTTTTGATGATGTCCGATCTAAACGGCATGGAGAGAAACGAGAGAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGGTCACTTATTGAGATGGAAGAGAAGTTGGATGAGCTGCTTCAATCCCAAGCTCATGTTTGGAACCATGCTTTGAAATTTATGAACTCCATGGCTCTTAAATGTGCTGTTGAATTGGGCATACCAGATGCTCAACACCTTGGCCGCCTCATGCGCCTCCTCGTCCATTCTGGCTTCTTTGATGCCAACGGGCAAGAGGACGTGAAATACAGCCTCACGCCCTCCTCTCGCCTTCTCCTCCGCCACACACACACCTACACCACTTTCCAAATGATACCCTTCTTGTGTCTTGCTTTGGATAAAGCTACAATTGCTTCTTTGGGAGCTTTGAGCGGTTGGCTTTGTAACTCAGATCAAGACCATAACTCCAGTTCCTTTGAAATGGCAAATGGGAAGCCCATATGGGAGTATGCTGCTCAGGAACCAAATTTTGGCAATTTGTTTCAACAGATAATGGTTTGTGATTCTGAATTGATTGGTAAAATAGTGATTGAGAAGTGTAGCAGAGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGGACATCACTTGCATTGTATTTGATCTCCCACAAGTGGTTTCCAACCAGCAACGAACTGCAAAAAACTTGAGCTTGATTACAACA

Protein sequence

MLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTETQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLSGWLCSSDQDHNSSSFEMANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTETQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLRSLIEMEEKLDELLQSQAHVWNHALKFMNSMALKCAVELGIPDAQHLGRLMRLLVHSGFFDANGQEDVKYSLTPSSRLLLRHTHTYTTFQMIPFLCLALDKATIASLGALSGWLCNSDQDHNSSSFEMANGKPIWEYAAQEPNFGNLFQQIMVCDSELIGKIVIEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPDITCIVFDLPQVVSNQQRTAKNLSLITT

Homology

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BLAST of Cp4.1LG16g04140 vs. Swiss-Prot
Match: ROMT_VITVI (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera GN=ROMT PE=1 SV=2)

HSP 1 Score: 243.0 bits (619), Expect = 7.5e-63
Identity = 120/229 (52.40%), Postives = 162/229 (70.74%), Query Frame = 1


Query: 107 LSGWLCSSDQDHNSSSFEMANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKCSG 166
           LS W     Q+ + + F  A  +S WDY+  EP   N F++AM  D+ LL  +++++  G
Sbjct: 133 LSAWF----QNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTSVLLKEGQG 192

Query: 167 VFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLES 226
           VF GL SLVDVGGGTG +A AIA AFPH+ C+V DL  VV+  Q + KNL++  GDM E+
Sbjct: 193 VFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGS-KNLNYFAGDMFEA 252

Query: 227 KIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTE 286
            IPPA+A++LKWILH+W +E+ +KILK+C++AIPS+  GGK+IIIDM++     D +STE
Sbjct: 253 -IPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQGDYKSTE 312

Query: 287 TQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLRSLIEM 336
           TQLFFD+ MM    G ER+E EW+ LF++AGFS Y I   LGLRSLIE+
Sbjct: 313 TQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEV 355

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BLAST of Cp4.1LG16g04140 vs. Swiss-Prot
Match: HTOMT_CATRO (Tabersonine 16-O-methyltransferase OS=Catharanthus roseus GN=16OMT PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.5e-58
Identity = 118/241 (48.96%), Postives = 164/241 (68.05%), Query Frame = 1


Query: 100 TIVSKLGLSGWLCSSD----QDHNSSSFEMANGKSMWDYSAQEPSFGNFFHQAMVCDSEL 159
           T+   + L  W   SD    +D +S++FE A+GK+ W YS++      FF++AM  DS+L
Sbjct: 117 TMADPVQLKAWESLSDWYQNEDDSSTAFETAHGKNFWGYSSEHMEHAEFFNEAMASDSQL 176

Query: 160 LGRIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKN 219
           + +++I +   +FEGL SLVD+GGGTG +A AIA+ FP + C+VFDLP VV+N + + +N
Sbjct: 177 ISKLLIGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLE-SKEN 236

Query: 220 LSFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVL 279
           + F+ GDM E KIP ANA+ LKWILH+W+DE  +KILK CK AIP  A+GGK+IIIDMV+
Sbjct: 237 VEFVAGDMFE-KIPSANAIFLKWILHDWNDEDCVKILKSCKKAIP--AKGGKVIIIDMVM 296

Query: 280 DNEVEDRESTETQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGL-RSLIE 336
            ++ +D    +TQ   D+ M+ +    ER E+EW  LF EAGFSDY I  KL   RSLIE
Sbjct: 297 YSDKKDDHLVKTQTSMDMAMLVNFAAKERCEKEWAFLFKEAGFSDYKIYPKLDFTRSLIE 353

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BLAST of Cp4.1LG16g04140 vs. Swiss-Prot
Match: I4OMT_GLYEC (Isoflavone 4'-O-methyltransferase OS=Glycyrrhiza echinata GN=HI4'OMT PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 5.7e-55
Identity = 108/222 (48.65%), Postives = 153/222 (68.92%), Query Frame = 1


Query: 116 QDHNSSSFEMANGKSMWDYSAQEPSFG--NFFHQAMVCDSELLGRIVIEKCSGVFEGLKS 175
           +D   + FE A G+S WD+  ++   G  + F +AM  DS++  ++ +++C  VFEGL+S
Sbjct: 147 EDKELTLFESATGESFWDFLNKDSESGTLSMFQEAMAADSQMF-KLALKECRHVFEGLES 206

Query: 176 LVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLESKIPPANA 235
           LVDVGGGTG +   I E FPH+ C+VFD PQVV N     +NL F+GGDM +S IPPA+A
Sbjct: 207 LVDVGGGTGGVTKLIHEEFPHLKCTVFDQPQVVGNLSGN-ENLKFVGGDMFKS-IPPADA 266

Query: 236 VMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTETQLFFDV 295
           V+LKW+LH+W+DE S+KILK  K+AI  + + GK+IIID+ +D    DRE TE QL +D+
Sbjct: 267 VLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVIIIDISIDEASGDRELTELQLDYDL 326

Query: 296 LMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLRSLIEM 336
           +M++  NG ER ++EW+ L  +AGFS Y I    G +SLIE+
Sbjct: 327 VMLTMFNGKEREKKEWEKLISDAGFSSYKITPICGFKSLIEV 365

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BLAST of Cp4.1LG16g04140 vs. Swiss-Prot
Match: MOMT_CATRO (Myricetin O-methyltransferase OS=Catharanthus roseus PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.7e-54
Identity = 110/233 (47.21%), Postives = 157/233 (67.38%), Query Frame = 1


Query: 107 LSGWLCSSD--QDHNSSSFEMANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKC 166
           L  W   S+  Q+ + ++FE A+GK+ WD+ A++  +G  F   M  DS L+ +++I + 
Sbjct: 117 LKAWNAMSEWFQNEDLTAFETAHGKNFWDFGAED-KYGKNFDGVMAADSILVSKMLIPEF 176

Query: 167 SGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDML 226
           + +FEGL SLVDVGGGTG +A AIA++FP + C+VFDLP VV+N + T +NL F+GGDM 
Sbjct: 177 NYLFEGLDSLVDVGGGTGTIAKAIAKSFPDLKCTVFDLPHVVANLEST-ENLEFVGGDMF 236

Query: 227 ESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVL--DNEVEDR 286
           E KIP ANA++LKWILH+W DE+ +K+LK C+ AIP + +GGK+I+I+ VL    + E+ 
Sbjct: 237 E-KIPSANAILLKWILHDWKDEECVKVLKMCRKAIPEKEKGGKVILIETVLMDSKKHENE 296

Query: 287 ESTETQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLRSLIEM 336
           E+ + Q+  D+ MM      ER E EW  LF EAGFS Y I   +  RS IE+
Sbjct: 297 EAVKAQISSDIDMMVFFTAKERTEEEWATLFREAGFSGYKIFPMIDFRSPIEV 346

[Close]

BLAST of Cp4.1LG16g04140 vs. Swiss-Prot
Match: I4OMT_LOTJA (Isoflavone 4'-O-methyltransferase OS=Lotus japonicus GN=HI4'OMT PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.7e-54
Identity = 105/215 (48.84%), Postives = 150/215 (69.77%), Query Frame = 1


Query: 123 FEMANGKSMWDYSAQEPSFG--NFFHQAMVCDSELLGRIVIEKCSGVFEGLKSLVDVGGG 182
           FE A G+S W++  +E      + F +AM  DS +  ++ +++C  VFEGL SLVDV GG
Sbjct: 152 FESATGESFWEFLNKETESDTLSMFQEAMAADSHMF-KLALKECKHVFEGLGSLVDVAGG 211

Query: 183 TGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLESKIPPANAVMLKWIL 242
            G +   I EAFPH+ C+VFD PQVV+N     +NL+F+GGDM +S +PPA+AV+LKW+L
Sbjct: 212 RGGVTKLIREAFPHVKCTVFDQPQVVANLTGD-ENLNFVGGDMFKS-VPPADAVLLKWVL 271

Query: 243 HNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTETQLFFDVLMMSDLN 302
           H+W+DE S+KILK CK+AI  R + GK+IIID+ +D   +DRE TE +L +D++M++  N
Sbjct: 272 HDWNDELSLKILKNCKEAISGRGKEGKVIIIDISIDETSDDRELTELKLDYDLVMLTMFN 331

Query: 303 GMERNEREWKNLFMEAGFSDYTIVSKLGLRSLIEM 336
           G ER ++EW+ L  +AGFS Y I    G +SLIE+
Sbjct: 332 GKEREKKEWEKLIYDAGFSSYKITPICGFKSLIEV 363

[Close]

BLAST of Cp4.1LG16g04140 vs. TrEMBL
Match: A0A0A0LMQ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G415520 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 7.4e-94
Identity = 175/248 (70.56%), Postives = 200/248 (80.65%), Query Frame = 1


Query: 91  FMEAGFSDYTIVSKLGLSGWLCSSDQD--HNSSSFEMANGKSMWDYSAQEPSFGNFFHQA 150
           F+       TI S   LS W CSS+ +  H S++FEMANGK +WDY+AQE SF N F Q 
Sbjct: 113 FLFLSLDKATIASFQNLSSWFCSSNNNGQHYSNAFEMANGKLIWDYAAQEQSFANLFQQT 172

Query: 151 MVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSN 210
           MVCDSE +G+IV E+CS VFEGLKSLVDVGGGTG M  AI EAFPHITC+VFDLPQV+SN
Sbjct: 173 MVCDSETIGKIVKEECSEVFEGLKSLVDVGGGTGAMGKAIVEAFPHITCTVFDLPQVISN 232

Query: 211 QQ-RTAKNLSFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGK 270
           Q  + AKNL F+ GDM E  IPPANAV+LKWILHNW+DEQSI+ILKKC+DAIPSR EGGK
Sbjct: 233 QPLQNAKNLRFVEGDMFEEIIPPANAVLLKWILHNWNDEQSIRILKKCRDAIPSRDEGGK 292

Query: 271 LIIIDMVLDNEVEDRESTETQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKL 330
           LIIIDMV++ + ED ESTETQL FD+LMM +LNG ERNE EWKNLFMEAGFS Y I+SKL
Sbjct: 293 LIIIDMVMERKKEDIESTETQLLFDLLMMVNLNGKERNENEWKNLFMEAGFSGYKIISKL 352

Query: 331 GLRSLIEM 336
           GLRSLIE+
Sbjct: 353 GLRSLIEV 360

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BLAST of Cp4.1LG16g04140 vs. TrEMBL
Match: A0A0B2RAF2_GLYSO (Isoflavone 7-O-methyltransferase OS=Glycine soja GN=glysoja_029967 PE=3 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 5.9e-83
Identity = 157/330 (47.58%), Postives = 224/330 (67.88%), Query Frame = 1


Query: 6   IPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTET 65
           IP A+AV+LKW+LH+WDDE  IKIL+KCKD+I S+  GGK+IIID V++ +++D + T+T
Sbjct: 243 IPQASAVLLKWVLHDWDDEDCIKILEKCKDSISSKGNGGKVIIIDTVINEKLDDPDMTQT 302

Query: 66  QLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLSGWLCSSDQDHNSSSFEM 125
           +L  D+++M  +NG ER+E+EWK LF EAGF  + I    G                   
Sbjct: 303 KLSLDIIVMLTMNGKERSEKEWKQLFTEAGFKHHKIFPIFGF------------------ 362

Query: 126 ANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGTGVMA 185
              +S+ ++  +  +    F++AM  DS+++G + ++ C+ VFEGL S+VDVGGGTG  A
Sbjct: 363 ---RSLIEFLRK--TLNILFNEAMASDSQMVG-LALKNCTSVFEGLDSMVDVGGGTGTTA 422

Query: 186 NAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLESKIPPANAVMLKWILHNWDD 245
             I +AFP + C V DLP VV N   T  NLSF+GGDM +S IP A+AV+LKW+LHNW D
Sbjct: 423 RIICDAFPELKCVVLDLPHVVENLMAT-NNLSFVGGDMFKS-IPQADAVLLKWVLHNWTD 482

Query: 246 EQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTETQLFFDVLMMSDLNGMERN 305
           E  IKILKKC+D+I S+   GK+IIID V++ +++D + T+T+L  D++M++ +NG ER 
Sbjct: 483 ENCIKILKKCRDSISSKGNSGKVIIIDTVINEKLDDPDMTQTKLSLDIIMLT-MNGRERT 542

Query: 306 EREWKNLFMEAGFSDYTIVSKLGLRSLIEM 336
           E++WK LF EAGF+ Y I    G RSLIE+
Sbjct: 543 EKDWKQLFTEAGFNHYKIFPIFGFRSLIEV 545

[Close]

BLAST of Cp4.1LG16g04140 vs. TrEMBL
Match: A0A067D323_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g023625mg PE=3 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 8.9e-71
Identity = 137/228 (60.09%), Postives = 170/228 (74.56%), Query Frame = 1


Query: 107 LSGWLCSSDQDHNSSSFEMANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKCSG 166
           L  WL    Q+ + S FE A+GK +WD  A EP F + F+  M+ DSEL+  IVI+ C  
Sbjct: 54  LGTWL----QNDDPSLFETAHGKKVWDRVADEPKFKSLFYDLMITDSELIAGIVIKDCKE 113

Query: 167 VFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLES 226
           VFEGLKSLVDV GGTG+MA AIA AFP I C+VFDLP VV N Q T  NL F+GG+M E+
Sbjct: 114 VFEGLKSLVDVAGGTGIMARAIATAFPDIKCTVFDLPHVVDNLQGTNDNLDFLGGNMFEA 173

Query: 227 KIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTE 286
            IP ANAV+LKWILHNW+DE+S+K+LKKCK+AIPS+ EGGK+IIIDM ++N+ +D+ES E
Sbjct: 174 -IPQANAVLLKWILHNWNDEESVKLLKKCKEAIPSKDEGGKVIIIDMAIENQSQDKESME 233

Query: 287 TQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLRSLIE 335
           TQL FD+LM+S   G ER+  +WK LF+ AGFS Y I   LG+RSLIE
Sbjct: 234 TQLCFDILMVSLFRGKERSVDDWKKLFLAAGFSHYKITPMLGVRSLIE 276

[Close]

BLAST of Cp4.1LG16g04140 vs. TrEMBL
Match: F6H5H6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g02840 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 6.3e-69
Identity = 126/229 (55.02%), Postives = 175/229 (76.42%), Query Frame = 1


Query: 107 LSGWLCSSDQDHNSSSFEMANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKCSG 166
           +S W     Q+ + + F+ A+G++ WDY   EP   NFF++AM  D+ L+  ++I+ C G
Sbjct: 133 VSAWF----QNDDPTPFDTAHGRTFWDYGGHEPKINNFFNEAMASDARLVTSVLIKDCKG 192

Query: 167 VFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLES 226
           VFEGL SLVDVGGGTG +A AIA AFPH+ C+VFDLP VV+  + + KNL+++GGDM + 
Sbjct: 193 VFEGLNSLVDVGGGTGTVAKAIANAFPHLNCTVFDLPHVVAGLEGS-KNLNYLGGDMFKG 252

Query: 227 KIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTE 286
            IPPA+A++LKWILH+W+DE+ +KIL++C+ AIPS+ +GGK+IIIDM+++N+  D ES E
Sbjct: 253 -IPPADAILLKWILHDWNDEECVKILQQCRQAIPSKEKGGKVIIIDMMMENQKGDDESME 312

Query: 287 TQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLRSLIEM 336
           TQLFFD+LMM  + G ERNE+EW+ LF++AGFS Y I   LGLRSLIE+
Sbjct: 313 TQLFFDMLMMILVTGQERNEKEWEKLFLDAGFSGYKITPILGLRSLIEV 355

[Close]

BLAST of Cp4.1LG16g04140 vs. TrEMBL
Match: A0A0D9XRC4_9ORYZ (Uncharacterized protein OS=Leersia perrieri PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 8.3e-69
Identity = 170/502 (33.86%), Postives = 259/502 (51.59%), Query Frame = 1


Query: 77  LNGMERNEREWKNLFMEAGFSDYTIVSKLGLSGWLCSS--DQDHNSSSFEMANGKSMWDY 136
           ++G+  +E   +  F+ A  S + + + LGL  W      + +   S FE  +G +++D 
Sbjct: 115 VDGVVADEHHSQTSFVLAATSRHYLEASLGLDEWFRKDVPEGEPLPSPFEDVHGAALFDE 174

Query: 137 SAQ--EPSFGNFFHQAMVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAF 196
           S    +P      ++ +     L    ++ +C  +F G+KSL D  GG G  A A+ +A+
Sbjct: 175 STPLLDPELDAVVNEGLAAHDNLGIGTILRECRNLFRGVKSLTDCCGGDGTTARAVVKAY 234

Query: 197 PHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKIL 256
           PHI C+V DLP+V+    +    ++++ GD+  + +PP+ AVMLK +LH+W D+  +KIL
Sbjct: 235 PHIKCTVLDLPKVIDKAPKD-DGVNYVAGDLFHT-VPPSQAVMLKLVLHHWSDDDCVKIL 294

Query: 257 KKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTETQLFFDVLMMSDLNGMERNEREWKNL 316
            +C+ AIPSR EGGK+III++++   +      E QL  D+LM+ +  G +R+E +W+NL
Sbjct: 295 AQCRKAIPSREEGGKVIIIEILVGPSLGP-VMFEAQLMMDMLMLVNTRGRQRDESDWENL 354

Query: 317 FMEAGFSDYTIVSKLGLRSLIEMEEKLD-ELLQSQAHVWNHALKFMNSMALKCAVELGIP 376
           F++AGFSDY IV  LG R    +    D ELLQ+QA +W H+L ++  MAL+CAV+LGIP
Sbjct: 355 FLKAGFSDYKIVKMLGARGPSHLIPPTDDELLQAQADLWRHSLSYLTPMALRCAVKLGIP 414

Query: 377 DAQH------------------------LGRLMRLLVHSGFFDANGQEDVK---YSLTPS 436
            A H                        L RLMRLLV+S  F            Y LTP 
Sbjct: 415 TAIHRAGGQSTLPDLITSLSLPSSKLPFLRRLMRLLVNSDIFTLTTTTTTGVHVYGLTPL 474

Query: 437 SRLLLRHTHTYTT------FQMIPFLCLALDKATIASLGALSGWLCNSDQDHNSSSFEMA 496
           S  L+   +               F+  A     + +   L  W   +D D+  S FE A
Sbjct: 475 SLFLVDGAYFADADVDDGHTNQSAFVLAATSPHYVEAALGLDDWF-RADDDY-VSPFEAA 534

Query: 497 NGKPIWEY--AAQEPNFGNLFQQIMVCDSELIGKIVIEKCSRVFEGLKSLVDVGGGTGVM 539
           +  P+     A  +     L  + +V  + +   + + +   VFEG++SLVD GGG G  
Sbjct: 535 HDAPLLHETRAVADAELSALVGEALVAQNHMGIGLALRESRAVFEGMESLVDCGGGDGAT 594

[Close]

BLAST of Cp4.1LG16g04140 vs. TAIR10
Match: AT4G35160.1 (AT4G35160.1 O-methyltransferase family protein)

HSP 1 Score: 141.0 bits (354), Expect = 2.3e-33
Identity = 78/237 (32.91%), Postives = 127/237 (53.59%), Query Frame = 1


Query: 105 LGLSGWLCSSDQDHNSSSFEMANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKC 164
           L LS  + S         F+  +GK +W ++   P   +  ++AM CD+  +   V   C
Sbjct: 145 LRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVVPRVAGAC 204

Query: 165 SGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDML 224
            G+F+G+ ++VDVGGGTG     + + FP I    FDLP V+   +     +  + GDM 
Sbjct: 205 HGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAE-VLDGVENVEGDMF 264

Query: 225 ESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDN------- 284
           +S IP  +A+ +KW+LH+W D+  IKILK CK+A+P     GK++I++ V+         
Sbjct: 265 DS-IPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNI--GKVLIVESVIGENKKTMIV 324

Query: 285 EVEDRESTETQLFFDVLMMSDLN-GMERNEREWKNLFMEAGFSDYTIVSKLGLRSLI 334
           +  D +    +L  D++MM+  + G ER  +EW  +  EAGF+ Y +     ++SLI
Sbjct: 325 DERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

[Close]

BLAST of Cp4.1LG16g04140 vs. TAIR10
Match: AT1G51990.1 (AT1G51990.1 O-methyltransferase family protein)

HSP 1 Score: 133.7 bits (335), Expect = 3.6e-31
Identity = 75/218 (34.40%), Postives = 129/218 (59.17%), Query Frame = 1


Query: 103 SKLGLSGWLCSSDQDHNSSSFEMANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIE 162
           +K G+  +L  + Q+  +S++E AN   +++Y  +  +    F+++M   + ++ + ++E
Sbjct: 132 AKGGVWSYLTEAIQEGGASAWERANEALIFEYMKKNENLKKIFNESMTNHTSIVMKKILE 191

Query: 163 KCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGD 222
              G FEG+   VDVGG  G     I   +PHI    FDLP +V    +    +  IGGD
Sbjct: 192 NYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQI-HGVEHIGGD 251

Query: 223 MLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDR 282
           M + +IP    +++KWILH+W+DE+ ++ILK CK A+P   E G++I+I+M++  EV + 
Sbjct: 252 MFD-EIPRGEVILMKWILHDWNDEKCVEILKNCKKALP---ETGRIIVIEMIVPREVSET 311

Query: 283 E-STETQLFFDVLMMS-DLNGMERNEREWKNLFMEAGF 319
           + +T+  L  D+ MMS    G ER ++E+++L  EAGF
Sbjct: 312 DLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGF 343

[Close]

BLAST of Cp4.1LG16g04140 vs. TAIR10
Match: AT4G35150.1 (AT4G35150.1 O-methyltransferase family protein)

HSP 1 Score: 130.6 bits (327), Expect = 3.0e-30
Identity = 74/215 (34.42%), Postives = 118/215 (54.88%), Query Frame = 1


Query: 127 NGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGTGVMAN 186
           +GK +W ++          ++AM CD+  +   V   C G+F+G+ ++VDVGGGTG    
Sbjct: 110 HGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQGLFDGVATVVDVGGGTGETMG 169

Query: 187 AIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLESKIPPANAVMLKWILHNWDDE 246
            + + FP I    FDLP V+   Q     +  + GDM +S IP ++AV++KW+LH+W D+
Sbjct: 170 ILVKEFPWIKGFNFDLPHVIEVAQ-VLDGVENVEGDMFDS-IPASDAVIIKWVLHDWGDK 229

Query: 247 QSIKILKKCKDAIPSRAEGGKLIIIDMVLDN-------EVEDRESTETQLFFDVLMMSDL 306
             IKILK CK+A+      GK++I++ V+         E  D +    +L  D++MM   
Sbjct: 230 DCIKILKNCKEAVLPNI--GKVLIVECVIGEKKNTMIAEERDDKLEHVRLQLDMVMMVHT 289

Query: 307 N-GMERNEREWKNLFMEAGFSDYTIVSKLGLRSLI 334
           + G ER  +EW  +  EAGF+ Y +     ++SLI
Sbjct: 290 STGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 320

[Close]

BLAST of Cp4.1LG16g04140 vs. TAIR10
Match: AT5G54160.1 (AT5G54160.1 O-methyltransferase 1)

HSP 1 Score: 129.4 bits (324), Expect = 6.8e-30
Identity = 74/218 (33.94%), Postives = 118/218 (54.13%), Query Frame = 1


Query: 123 FEMANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGTG 182
           F  A G S ++Y   +P F   F+  M   S +  + ++E   G FEGL SLVDVGGG G
Sbjct: 152 FNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGIG 211

Query: 183 VMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLESKIPPANAVMLKWILHN 242
                I   +P++    FDLP V+ +   +   +  +GGDM  S +P  +A+ +KWI H+
Sbjct: 212 ATLKMIVSKYPNLKGINFDLPHVIEDAP-SHPGIEHVGGDMFVS-VPKGDAIFMKWICHD 271

Query: 243 WDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTETQLFFDVLMMS-DLNG 302
           W DE  +K LK C +++P   E GK+I+ + +L    +   ST+  +  D +M++ +  G
Sbjct: 272 WSDEHCVKFLKNCYESLP---EDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGG 331

Query: 303 MERNEREWKNLFMEAGFSDYTIVSKLGLRSLIEMEEKL 340
            ER E+E++ L   +GF    +V      +LIE+ +KL
Sbjct: 332 KERTEKEFEALAKASGFKGIKVVCDAFGVNLIELLKKL 363

[Close]

BLAST of Cp4.1LG16g04140 vs. TAIR10
Match: AT1G77530.1 (AT1G77530.1 O-methyltransferase family protein)

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-28
Identity = 76/218 (34.86%), Postives = 123/218 (56.42%), Query Frame = 1


Query: 122 SFEMANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGT 181
           +F  A+   +++Y + +  F   FH+AM   S ++ + V+E+  G FE + +LVDVGGG 
Sbjct: 170 AFSSAHDMRLFEYISSDDQFSKLFHRAMSESSTMVMKKVLEEYRG-FEDVNTLVDVGGGI 229

Query: 182 GVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLESKIPPANAVMLKWILH 241
           G +   I   +PHI    FDL QV++ Q      +  + GDM   ++P  +A+ +KWILH
Sbjct: 230 GTILGLITSKYPHIKGVNFDLAQVLT-QAPFYPGVKHVSGDMF-IEVPKGDAIFMKWILH 289

Query: 242 NWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRE-STETQLFFDVLMMSDLN 301
           +W DE  IKILK C  ++P   E GK+II++M+   E +  + S  T L  D+LM++  +
Sbjct: 290 DWGDEDCIKILKNCWKSLP---EKGKVIIVEMITPMEPKPNDFSCNTVLGMDLLMLTQCS 349

Query: 302 -GMERNEREWKNLFMEAGFSDYTIVSKLGLRSLIEMEE 338
            G ER+  +++NL   +GF    I+      S+IE  +
Sbjct: 350 GGKERSLSQFENLAFASGFLLCEIICLSYSYSVIEFHK 381

[Close]

BLAST of Cp4.1LG16g04140 vs. NCBI nr
Match: gi|449468394|ref|XP_004151906.1| (PREDICTED: trans-resveratrol di-O-methyltransferase [Cucumis sativus])

HSP 1 Score: 352.8 bits (904), Expect = 1.1e-93
Identity = 175/248 (70.56%), Postives = 200/248 (80.65%), Query Frame = 1


Query: 91  FMEAGFSDYTIVSKLGLSGWLCSSDQD--HNSSSFEMANGKSMWDYSAQEPSFGNFFHQA 150
           F+       TI S   LS W CSS+ +  H S++FEMANGK +WDY+AQE SF N F Q 
Sbjct: 113 FLFLSLDKATIASFQNLSSWFCSSNNNGQHYSNAFEMANGKLIWDYAAQEQSFANLFQQT 172

Query: 151 MVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSN 210
           MVCDSE +G+IV E+CS VFEGLKSLVDVGGGTG M  AI EAFPHITC+VFDLPQV+SN
Sbjct: 173 MVCDSETIGKIVKEECSEVFEGLKSLVDVGGGTGAMGKAIVEAFPHITCTVFDLPQVISN 232

Query: 211 QQ-RTAKNLSFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGK 270
           Q  + AKNL F+ GDM E  IPPANAV+LKWILHNW+DEQSI+ILKKC+DAIPSR EGGK
Sbjct: 233 QPLQNAKNLRFVEGDMFEEIIPPANAVLLKWILHNWNDEQSIRILKKCRDAIPSRDEGGK 292

Query: 271 LIIIDMVLDNEVEDRESTETQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKL 330
           LIIIDMV++ + ED ESTETQL FD+LMM +LNG ERNE EWKNLFMEAGFS Y I+SKL
Sbjct: 293 LIIIDMVMERKKEDIESTETQLLFDLLMMVNLNGKERNENEWKNLFMEAGFSGYKIISKL 352

Query: 331 GLRSLIEM 336
           GLRSLIE+
Sbjct: 353 GLRSLIEV 360

[Close]

BLAST of Cp4.1LG16g04140 vs. NCBI nr
Match: gi|659111857|ref|XP_008455944.1| (PREDICTED: trans-resveratrol di-O-methyltransferase-like isoform X2 [Cucumis melo])

HSP 1 Score: 337.8 bits (865), Expect = 3.6e-89
Identity = 169/248 (68.15%), Postives = 201/248 (81.05%), Query Frame = 1


Query: 91  FMEAGFSDYTIVSKLGLSGWLCSSDQD--HNSSSFEMANGKSMWDYSAQEPSFGNFFHQA 150
           F+       TI S   LS W CSS+ +  + S++FEMANGK +W+Y+AQE +F N F Q 
Sbjct: 113 FLFLALDKSTIASFQSLSSWFCSSNNNGQNYSNAFEMANGKLLWEYAAQEQTFANLFQQT 172

Query: 151 MVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSN 210
           MVCDSE++G+IV E CS VFEGLKSLVDVGGGTGVM  AI EAFPHITC+VFDLPQV+SN
Sbjct: 173 MVCDSEMIGKIVKE-CSEVFEGLKSLVDVGGGTGVMGKAIVEAFPHITCTVFDLPQVISN 232

Query: 211 QQ-RTAKNLSFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGK 270
           Q  + AKNL F+ GDM E  IP ANAV+LKWILH+W+DEQSIKILKKCKDAIPSR +GGK
Sbjct: 233 QPLQNAKNLRFVEGDMFEEIIPLANAVLLKWILHDWNDEQSIKILKKCKDAIPSREKGGK 292

Query: 271 LIIIDMVLDNEVEDRESTETQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKL 330
           LIIID+V++++ E++ESTETQL FDVLMM +L G ERNE EWKNLFMEAGFS Y I+SKL
Sbjct: 293 LIIIDIVMEDKKEEKESTETQLLFDVLMMVNLGGKERNENEWKNLFMEAGFSGYKIISKL 352

Query: 331 GLRSLIEM 336
           GLRS+IE+
Sbjct: 353 GLRSVIEV 359

[Close]

BLAST of Cp4.1LG16g04140 vs. NCBI nr
Match: gi|659111853|ref|XP_008455942.1| (PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 323.2 bits (827), Expect = 9.1e-85
Identity = 159/248 (64.11%), Postives = 197/248 (79.44%), Query Frame = 1


Query: 91  FMEAGFSDYTIVSKLGLSGWLCSSDQD--HNSSSFEMANGKSMWDYSAQEPSFGNFFHQA 150
           F+        I S   LS W  SS+ +  + S++FEMANGK +W+Y+ QE +F + F Q 
Sbjct: 100 FLFVSLDKAIIASFQSLSSWFRSSNNNGQNYSNAFEMANGKLLWEYAPQEQTFASLFQQT 159

Query: 151 MVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSN 210
           MVCDSE++G+IV ++CS VFEGLKSLVDVGGGTGVM  AI EAFPHITC+VFDLPQV+SN
Sbjct: 160 MVCDSEMIGKIVKQECSEVFEGLKSLVDVGGGTGVMGKAIVEAFPHITCTVFDLPQVISN 219

Query: 211 QQ-RTAKNLSFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGK 270
           Q  + AKNL F+ GDM E  IPPANA++LKWILH+W+DEQ+I+ILKKCKDAI SR + GK
Sbjct: 220 QPLQNAKNLRFVEGDMFEEIIPPANAILLKWILHDWNDEQNIRILKKCKDAIRSREKCGK 279

Query: 271 LIIIDMVLDNEVEDRESTETQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKL 330
           LIIID+V++++ E++ESTETQL FDVLMM +L G E NE EWKNLFMEAGFS Y I+SKL
Sbjct: 280 LIIIDIVMEDKKEEKESTETQLLFDVLMMVNLGGKEMNENEWKNLFMEAGFSGYKIISKL 339

Query: 331 GLRSLIEM 336
           GLRS+IE+
Sbjct: 340 GLRSVIEV 347

[Close]

BLAST of Cp4.1LG16g04140 vs. NCBI nr
Match: gi|734398572|gb|KHN30530.1| (Isoflavone 7-O-methyltransferase [Glycine soja])

HSP 1 Score: 316.6 bits (810), Expect = 8.5e-83
Identity = 157/330 (47.58%), Postives = 224/330 (67.88%), Query Frame = 1


Query: 6   IPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTET 65
           IP A+AV+LKW+LH+WDDE  IKIL+KCKD+I S+  GGK+IIID V++ +++D + T+T
Sbjct: 243 IPQASAVLLKWVLHDWDDEDCIKILEKCKDSISSKGNGGKVIIIDTVINEKLDDPDMTQT 302

Query: 66  QLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLSGWLCSSDQDHNSSSFEM 125
           +L  D+++M  +NG ER+E+EWK LF EAGF  + I    G                   
Sbjct: 303 KLSLDIIVMLTMNGKERSEKEWKQLFTEAGFKHHKIFPIFGF------------------ 362

Query: 126 ANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKCSGVFEGLKSLVDVGGGTGVMA 185
              +S+ ++  +  +    F++AM  DS+++G + ++ C+ VFEGL S+VDVGGGTG  A
Sbjct: 363 ---RSLIEFLRK--TLNILFNEAMASDSQMVG-LALKNCTSVFEGLDSMVDVGGGTGTTA 422

Query: 186 NAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLESKIPPANAVMLKWILHNWDD 245
             I +AFP + C V DLP VV N   T  NLSF+GGDM +S IP A+AV+LKW+LHNW D
Sbjct: 423 RIICDAFPELKCVVLDLPHVVENLMAT-NNLSFVGGDMFKS-IPQADAVLLKWVLHNWTD 482

Query: 246 EQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTETQLFFDVLMMSDLNGMERN 305
           E  IKILKKC+D+I S+   GK+IIID V++ +++D + T+T+L  D++M++ +NG ER 
Sbjct: 483 ENCIKILKKCRDSISSKGNSGKVIIIDTVINEKLDDPDMTQTKLSLDIIMLT-MNGRERT 542

Query: 306 EREWKNLFMEAGFSDYTIVSKLGLRSLIEM 336
           E++WK LF EAGF+ Y I    G RSLIE+
Sbjct: 543 EKDWKQLFTEAGFNHYKIFPIFGFRSLIEV 545

[Close]

BLAST of Cp4.1LG16g04140 vs. NCBI nr
Match: gi|645245912|ref|XP_008229106.1| (PREDICTED: trans-resveratrol di-O-methyltransferase-like [Prunus mume])

HSP 1 Score: 278.9 bits (712), Expect = 2.0e-71
Identity = 149/290 (51.38%), Postives = 197/290 (67.93%), Query Frame = 1


Query: 52  VLDNEVEDRESTETQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLSGWL 111
           ++  +++D E  +T + + +   S L   E      + L M     D  +     L G  
Sbjct: 105 IVHQDLDDEEEEKTVVVYRLTPASRLLLKESPLSTTQFLLM---ILDPVVTDPFHLMGTW 164

Query: 112 CS----SDQDHNSSSFEMANGKSMWDYSAQEPSFGNFFHQAMVCDSELLGRIVIEKCSGV 171
           C      +QDH +S FEMA+G+  W  +AQ+P FG+ F++AM  DS+LL R V+E+C GV
Sbjct: 165 CQMNNHGNQDHPASPFEMAHGRPFWGLAAQQPKFGSLFNEAMEADSQLLARAVVEECEGV 224

Query: 172 FEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQRTAKNLSFIGGDMLESK 231
           FEGL SLVDVGGGTG MA AIA+AFP+I C+VFD P VV+N Q T  NL F+GGDM E K
Sbjct: 225 FEGLNSLVDVGGGTGTMAKAIAKAFPNINCTVFDQPHVVANLQGT-HNLDFVGGDMFE-K 284

Query: 232 IPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIID--MVLDNEVEDREST 291
           IPPANA+ LKWILH+W DE+S+KILKK ++AI S+  GGK+II+D  + +DN+  D++S 
Sbjct: 285 IPPANAIFLKWILHDWSDEESVKILKKSREAILSKNGGGKVIILDINVSVDNKKMDKKSI 344

Query: 292 ETQLFFDVLMMSDLNGMERNEREWKNLFMEAGFSDYTIVSKLGLRSLIEM 336
           ETQL +D+LMM DLNG ER+E EW+ LF+ AGFS Y I   LGLRSLIE+
Sbjct: 345 ETQLMWDILMMVDLNGKERSEAEWEKLFLTAGFSHYKITHILGLRSLIEV 389

The following BLAST results are available for this feature:

BLAST of Cp4.1LG16g04140 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ROMT_VITVI	7.5e-63	52.40	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera GN=ROMT PE=1 SV=2	[more]
HTOMT_CATRO	5.5e-58	48.96	Tabersonine 16-O-methyltransferase OS=Catharanthus roseus GN=16OMT PE=1 SV=1	[more]
I4OMT_GLYEC	5.7e-55	48.65	Isoflavone 4'-O-methyltransferase OS=Glycyrrhiza echinata GN=HI4'OMT PE=1 SV=1	[more]
MOMT_CATRO	3.7e-54	47.21	Myricetin O-methyltransferase OS=Catharanthus roseus PE=1 SV=1	[more]
I4OMT_LOTJA	3.7e-54	48.84	Isoflavone 4'-O-methyltransferase OS=Lotus japonicus GN=HI4'OMT PE=1 SV=1	[more]

BLAST of Cp4.1LG16g04140 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMQ9_CUCSA	7.4e-94	70.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G415520 PE=3 SV=1	[more]
A0A0B2RAF2_GLYSO	5.9e-83	47.58	Isoflavone 7-O-methyltransferase OS=Glycine soja GN=glysoja_029967 PE=3 SV=1	[more]
A0A067D323_CITSI	8.9e-71	60.09	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g023625mg PE=3 SV=1	[more]
F6H5H6_VITVI	6.3e-69	55.02	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g02840 PE=3 SV=...	[more]
A0A0D9XRC4_9ORYZ	8.3e-69	33.86	Uncharacterized protein OS=Leersia perrieri PE=3 SV=1	[more]

BLAST of Cp4.1LG16g04140 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G35160.1	2.3e-33	32.91	O-methyltransferase family protein	[more]
AT1G51990.1	3.6e-31	34.40	O-methyltransferase family protein	[more]
AT4G35150.1	3.0e-30	34.42	O-methyltransferase family protein	[more]
AT5G54160.1	6.8e-30	33.94	O-methyltransferase 1	[more]
AT1G77530.1	1.7e-28	34.86	O-methyltransferase family protein	[more]

BLAST of Cp4.1LG16g04140 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449468394\|ref\|XP_004151906.1\|	1.1e-93	70.56	PREDICTED: trans-resveratrol di-O-methyltransferase [Cucumis sativus]	[more]
gi\|659111857\|ref\|XP_008455944.1\|	3.6e-89	68.15	PREDICTED: trans-resveratrol di-O-methyltransferase-like isoform X2 [Cucumis mel...	[more]
gi\|659111853\|ref\|XP_008455942.1\|	9.1e-85	64.11	PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis melo]	[more]
gi\|734398572\|gb\|KHN30530.1\|	8.5e-83	47.58	Isoflavone 7-O-methyltransferase [Glycine soja]	[more]
gi\|645245912\|ref\|XP_008229106.1\|	2.0e-71	51.38	PREDICTED: trans-resveratrol di-O-methyltransferase-like [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008168	methyltransferase activity
GO:0008171	O-methyltransferase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR016461	O-MeTrfase_COMT
IPR011991	Winged helix-turn-helix DNA-binding domain
IPR001077	O_MeTrfase_2

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008168	methyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG16g04140.1	Cp4.1LG16g04140.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001077	O-methyltransferase, family 2	PFAM	PF00891	Methyltransf_2	coord: 1..99 score: 8.2E-26coord: 427..543 score: 5.1E-23coord: 118..321 score: 3.1
IPR011991	Winged helix-turn-helix DNA-binding domain	GENE3D	G3DSA:1.10.10.10		coord: 374..408 score: 1.2E-8coord: 336..373 score: 2.1
IPR011991	Winged helix-turn-helix DNA-binding domain	unknown	SSF46785	"Winged helix" DNA-binding domain	coord: 339..411 score: 5.44
IPR016461	O-methyltransferase COMT-type	PROFILE	PS51683	SAM_OMT_II	coord: 329..553 score: 27.305coord: 1..337 score: 55
None	No IPR available	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 107..357 score: 1.2E
None	No IPR available	PANTHER	PTHR11746:SF99	SUBFAMILY NOT NAMED	coord: 107..357 score: 1.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG16g04140	CmaCh20G005800	Cucurbita maxima (Rimu)	cmacpeB562
Cp4.1LG16g04140	CmoCh20G006530	Cucurbita moschata (Rifu)	cmocpeB517
Cp4.1LG16g04140	MELO3C018855	Melon (DHL92) v3.5.1	cpemeB273
Cp4.1LG16g04140	Lsi11G014110	Bottle gourd (USVL1VR-Ls)	cpelsiB233
Cp4.1LG16g04140	MELO3C018855.2	Melon (DHL92) v3.6.1	cpemedB317
Cp4.1LG16g04140	MELO3C019324.2	Melon (DHL92) v3.6.1	cpemedB302
Cp4.1LG16g04140	CsaV3_2G034540	Cucumber (Chinese Long) v3	cpecucB0336
Cp4.1LG16g04140	CsaV3_7G003180	Cucumber (Chinese Long) v3	cpecucB0367
Cp4.1LG16g04140	Bhi10G001068	Wax gourd	cpewgoB0355
Cp4.1LG16g04140	Bhi05G001219	Wax gourd	cpewgoB0349
Cp4.1LG16g04140	CsGy2G025620	Cucumber (Gy14) v2	cgybcpeB195
Cp4.1LG16g04140	CsGy7G002950	Cucumber (Gy14) v2	cgybcpeB919

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG16g04140	Cp4.1LG15g07240	Cucurbita pepo (Zucchini)	cpecpeB255

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG16g04140	Cucumber (Gy14) v2	cgybcpeB766
Cp4.1LG16g04140	Melon (DHL92) v3.6.1	cpemedB329
Cp4.1LG16g04140	Silver-seed gourd	carcpeB0282
Cp4.1LG16g04140	Silver-seed gourd	carcpeB0465
Cp4.1LG16g04140	Silver-seed gourd	carcpeB1133
Cp4.1LG16g04140	Cucumber (Chinese Long) v3	cpecucB0358
Cp4.1LG16g04140	Cucurbita pepo (Zucchini)	cpecpeB069
Cp4.1LG16g04140	Cucurbita pepo (Zucchini)	cpecpeB304
Cp4.1LG16g04140	Cucurbita pepo (Zucchini)	cpecpeB311
Cp4.1LG16g04140	Cucurbita maxima (Rimu)	cmacpeB122
Cp4.1LG16g04140	Cucurbita maxima (Rimu)	cmacpeB525
Cp4.1LG16g04140	Cucurbita maxima (Rimu)	cmacpeB628
Cp4.1LG16g04140	Cucurbita moschata (Rifu)	cmocpeB100
Cp4.1LG16g04140	Cucurbita moschata (Rifu)	cmocpeB483
Cp4.1LG16g04140	Cucurbita moschata (Rifu)	cmocpeB577
Cp4.1LG16g04140	Wild cucumber (PI 183967)	cpecpiB271
Cp4.1LG16g04140	Wild cucumber (PI 183967)	cpecpiB301
Cp4.1LG16g04140	Cucumber (Chinese Long) v2	cpecuB269
Cp4.1LG16g04140	Cucumber (Chinese Long) v2	cpecuB298
Cp4.1LG16g04140	Bottle gourd (USVL1VR-Ls)	cpelsiB221
Cp4.1LG16g04140	Watermelon (Charleston Gray)	cpewcgB253
Cp4.1LG16g04140	Watermelon (Charleston Gray)	cpewcgB267
Cp4.1LG16g04140	Watermelon (Charleston Gray)	cpewcgB272
Cp4.1LG16g04140	Watermelon (97103) v1	cpewmB283
Cp4.1LG16g04140	Watermelon (97103) v1	cpewmB305
Cp4.1LG16g04140	Watermelon (97103) v1	cpewmB308
Cp4.1LG16g04140	Melon (DHL92) v3.5.1	cpemeB257